CN1570075A - Microbial cultivation sampling device - Google Patents
Microbial cultivation sampling device Download PDFInfo
- Publication number
- CN1570075A CN1570075A CN 03145940 CN03145940A CN1570075A CN 1570075 A CN1570075 A CN 1570075A CN 03145940 CN03145940 CN 03145940 CN 03145940 A CN03145940 A CN 03145940A CN 1570075 A CN1570075 A CN 1570075A
- Authority
- CN
- China
- Prior art keywords
- sample
- microorganism
- sampling unit
- inlet
- culture dish
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
Abstract
The invention discloses a microbial cultivation sampling device which comprises an example assembler, a culture plate and a filter liquor container, through which liquid example can be analyzed, an example injector may also be provided.
Description
Technical field
The invention relates to the cultivation sampling unit of a kind of microorganism, refer in particular to a kind of cultivation sampling unit that has with the microorganism of the isolating sample collector of a microorganism filter membrane culture dish, can be used for filter analysis and have the positive pressure current and the analyzing liquid sample to be filtered of negative pressure need be provided.
Background technology
Along with thin film transistor (thin-film transistors; TFTs) the quick progress of manufacturing technology has possessed frivolous, power saving and has not had liquid-crystal display (the liquid crystal display of advantages such as width of cloth ray; LCD), be to be applied in large quantities in the various electronic products such as counter, personal digital aid (PDA) (PDA), wrist-watch, mobile computer, digital camera, liquid-crystal display and mobile telephone.The manufacturing technology of liquid-crystal display mainly can be divided into:
(1) matrix processing procedure (Array process);
(2) panel processing procedure (Panel process);
(3) module processing procedure (Module process).
In above-mentioned many processing procedures, regular meeting uses pure water, and for example ultrapure water (UPW) or deionized water (DIW) clean the TFT panel, or the TFT panel also need add pure water and dilute these chemical agent product when using some chemical agent product.Since still contain microorganism in the pure water, and its diameter just looks like that particle is the same all at least about more than 1 micron, if the microorganism in the pure water is trapped on the panel, the situation that will cause panel to be short-circuited and to break, and have influence on process quality.Therefore, employed pure water in processing procedure must detect through content of microorganisms earlier, determines its contained microbial bacteria number in tolerance band, and for example every liter of interior contained bacterium number of pure water just can use in 50.
When the microbiological specimens that carries out pure water was analyzed, main purpose was to calculate per 100 milliliters or every liter of interior contained bacterium number of pure water.In the pre-treatment mode that microbiological specimens is analyzed, for the sample water of using the sampling sterilizing bag, because it does not have hydraulic pressure, can't pass through the microorganism filter membrane, so be by once the Millipore of particular design Milliflex-100 detection system at present, pure water vacuumizing filtration with the desire analysis, bacterium is stayed on the filter membrane, through method for cultivation of bacteria, allow the bacterium on this filter membrane form bacterium colony, remove to calculate the number of bacterium colony again, promptly know contained number of bacteria (bacterium can form a bacterium colony) in per 100 milliliters of this pure water sample.
Fig. 1 is the simple and easy synoptic diagram of this Millipore Milliflex-100 detection system 10, this system 10 comprises a microorganism filter membrane culture dish 11 and a Millipore vacuum pump 12, wherein this microorganism filter membrane culture dish 11 is integrally formed water containers that a bottom has a microorganism filter membrane 110, have two filter membrane microscope carriers 120 on this vacuum pump 12, the bore of this filter membrane microscope carrier 120 must be consistent with the bore of this microorganism filter membrane 110.
During use, at first, microorganism filter membrane 110 parts of this microorganism filter membrane culture dish 11 are inserted in arbitrary filter membrane microscope carrier 120 on this vacuum pump 12, and a waste water container 13 are connected to the wastewater outlet of this vacuum pump 12;
Then, the sample water of one sampling sterilizing bag is poured in the water container of this microorganism filter membrane culture dish 11, carry out vacuumizing filtration (forming a negative pressure), this sample water is detached by this microorganism filter membrane 110 be disposed to this waste water container 13, the bacterium in this sample water is then stayed on this filter membrane 110;
Afterwards, take out this microorganism filter membrane culture dish 11 from this filter membrane microscope carrier 120, downcut the filter membrane 110 of these microorganism filter membrane culture dish 11 bottoms, and this filter membrane 110 put a substrate disc culture dish (not shown), this substrate disc culture dish has a substratum inlet (not shown), pour into substratum from this inlet, form bacterium colony, so that calculate the number of bacterium with the bacterium of cultivating on this filter membrane 110.Its major defect is:
Because this Millipore Milliflex-100 detection system 10 is a corollary apparatus (kit), promptly this microorganism filter membrane culture dish 11 this Millipore vacuum pump 12 of must arranging in pairs or groups uses together, the price height, and this microorganism filter membrane culture dish 11 promptly abandons behind the filter membrane 110 that downcuts its bottom, can't re-use.Therefore, the employed filtration culture systems of being badly in need of existing microbiological specimens is analyzed of pre-treatment step proposes to improve, so that can effectively save the processing procedure cost.
Summary of the invention
The object of the present invention is to provide the cultivation sampling unit of a kind of microorganism, the sample divider that it had is reusable, and the cultivation sampling unit of this microorganism can be used for filter analysis and has the positive pressure current and the analyzing liquid sample to be filtered of negative pressure need be provided.
Another object of the present invention is to provide the cultivation sampling unit of a kind of microorganism, its general vacuum suction device of can arranging in pairs or groups, the pull of vacuum of generation negative pressure reaches low price and reaches the purpose that significantly reduces the required cost of microbiological analysis.
The object of the present invention is achieved like this: the cultivation sampling unit of a kind of microorganism is characterized in that: it comprises sample divider, culture dish and filtrate container; This sample divider is the liquid sample that contains microorganism in order to collection, and it comprises a sample injector in addition; This culture dish is to comprise an inlet, a filter membrane and a discharge outlet, and this inlet is to match with this sample injector, and to receive from effusive this liquid sample of this sample injector, this filter membrane is to be located between this inlet and this discharge outlet; This filtrate container is to combine with this discharge outlet, its with this sample divider between be to form a relative negative pressure, make and enter this culture dish inside via this inlet from effusive this liquid sample of this sample injector, be expelled to this filtrate container by this discharge outlet after the filtration through this filter membrane, this microorganism is that the filter membrane that is left on this culture dish is cultivated.
Formed relative negative pressure between this filtrate container and this sample divider is to form by making the liquid sample itself that is collected in this sample divider have the positive pressure that flows out into this culture dish inside from this sample injector.Formed relative negative pressure between this filtrate container and this sample divider is by connecting a vacuum suction device at this filtrate container, carries out vacuum suction and forms.This sample injector is a needle tubing, and its end has an acicular pores, and this needle tubing is the opening that is used for inserting this inlet and covers this inlet.This filtrate container is an erlenmeyer flask, and this erlenmeyer flask is connected with this discharge outlet by a pipe connecting, and the side of this erlenmeyer flask has an opening and is connected with this vacuum suction device, so that form vacuum suction.The bore of this inlet is 4mm, and the bore of this acicular pores is between 1-3mm.This sample liquids is a ultrapure water.This sample liquids is a deionized water.This liquid sample is from the sampling sterilizing bag.
In the cultivation sampling unit of microorganism of the present invention, formed relative negative pressure between this filtrate container and this sample divider, can form by making the liquid sample itself that is collected in this sample divider have the positive pressure that flows out into this culture dish inside from this sample injector, or, carry out vacuum suction and form by connecting a vacuum suction device at this filtrate container.
Describe in detail below in conjunction with preferred embodiment and accompanying drawing.
Description of drawings
Fig. 1 is the simple and easy synoptic diagram of the Millipore Milliflex-100 detection system of conventional art;
Fig. 2 is the synoptic diagram of preferable enforcement aspect of the cultivation sampling unit of microorganism of the present invention.
Embodiment
Consult shown in Figure 2ly, the cultivation sampling unit 50 of microorganism of the present invention comprises a sample collector 51, a culture dish 52 and a filtrate container (a for example erlenmeyer flask 53).Utilize the cultivation sampling unit 50 of this microorganism, promptly can be used for filter analysis and have the analyzing liquid sample to be filtered of positive pressure current, for example water that disengages via water tap institute high pressure; Or as shown in Figure 2, as long as the cultivation sampling unit 50 of this microorganism is between this filtrate container and this sample divider, install general vacuum suction device 54 again, can produce the pull of vacuum of negative pressure, come filter analysis that the analyzing liquid sample to be filtered of negative pressure need be provided, the sample water in the sterilizing bag of for example taking a sample or flowed into water in the process work bench tank (tank).
In the cultivation sampling unit 50 of microorganism, sample divider 51 comprises that a sample takes in a time meter 511 and a sample injector 512, wherein, it is in order to receive a liquid sample 40 that contains microorganism that this sample is taken in time meter 511, for example ultrapure water or deionized water, and this sample is taken in time meter 511 and is had graduation apparatus, can directly measure the volume content of the liquid sample 40 of impouring, and the bottom that this sample injector 512 is with this sample is taken in time meter 511 is connected, this sample injector 511 is needle tubings, is used for inserting this inlet, and covers the opening of this inlet, the end of this needle tubing has an acicular pores 513, and bore is in about 1-3mm scope.
The upper surface of this culture dish 52 has an outstanding inlet 521, for avoiding outside contamination, the bore of this inlet 521 is very little, be about about 4mm, acicular pores 513 effusive these liquid samples from this sample injector 511, inject these culture dish 52 inside via this inlet 521, filter membrane by this culture dish 52 filters, the negative pressure of using vacuum suction device 54 to be applied by the downward positive pressure of this liquid sample or below this culture dish 52, the filter membrane of this culture dish 52 will leach the interior microorganism of this liquid sample thereon, the filtrate of this liquid sample is then flowed out from these culture dish 52 lower surfaces, is received by this erlenmeyer flask 53.
Lower surface at this culture dish 52 has a discharge outlet 522, this erlenmeyer flask 53 is connected with the discharge outlet 522 of the lower surface of this culture dish 52 by a pipe connecting 531, and the side of this erlenmeyer flask 53 has an opening 532 and is connected with this vacuum suction device 54, so that carry out vacuum suction, below this culture dish 52, form negative pressure, quicken the filtration velocity of this sample.
The sample divider of the cultivation sampling unit of microorganism of the present invention is reusable, when not using, this sample divider can be soaked in H
2O
2In, keep complete sterilization; And desire is when using, again from H
2O
2The middle taking-up gets final product with pure water rinsing.
And the cultivation sampling unit of the microorganism of the present invention general vacuum suction device of can arranging in pairs or groups uses.Therefore, the low price of the cultivation sampling unit of whole microorganism compared to the employed Millipore Milliflex-100 of conventional art detection system, can reduce about 250,000 NT dollars of the makers' microbiological analysis cost of a liquid-crystal display every year.
Moreover, the cultivation sampling unit of microorganism of the present invention, for positive pressure current and need provide the analytic sample to be filtered of negative pressure all suitable itself are provided, and the microbiological analysis result is also good.
By the cultivation sampling unit of microorganism of the present invention, not only be applicable to the analyzing liquid sample to be filtered that has the positive pressure current and negative pressure need be provided, and can significantly reduce the required cost of microbiological analysis.
The above, the present invention utilizes preferred embodiment to describe in detail, and unrestricted protection scope of the present invention.Know this type of skill personage and all can understand, suitably do slightly change and adjustment, will not break away from spirit of the present invention and protection domain.
Claims (9)
1, the cultivation sampling unit of a kind of microorganism, it is characterized in that: it comprises sample divider, culture dish and filtrate container; This sample divider is the liquid sample that contains microorganism in order to collection, and it comprises a sample injector in addition; This culture dish is to comprise an inlet, a filter membrane and a discharge outlet, and this inlet is to match with this sample injector, and to receive from effusive this liquid sample of this sample injector, this filter membrane is to be located between this inlet and this discharge outlet; This filtrate container is to combine with this discharge outlet, its with this sample divider between be to form a relative negative pressure, make and enter this culture dish inside via this inlet from effusive this liquid sample of this sample injector, be expelled to this filtrate container by this discharge outlet after the filtration through this filter membrane, this microorganism is that the filter membrane that is left on this culture dish is cultivated.
2, the cultivation sampling unit of microorganism according to claim 1, it is characterized in that: formed relative negative pressure between this filtrate container and this sample divider is to form by making the liquid sample itself that is collected in this sample divider have the positive pressure that flows out into this culture dish inside from this sample injector.
3, the cultivation sampling unit of microorganism according to claim 1 is characterized in that: formed relative negative pressure between this filtrate container and this sample divider is by connecting a vacuum suction device at this filtrate container, carries out vacuum suction and forms.
4, the cultivation sampling unit of microorganism according to claim 1 is characterized in that: this sample injector is a needle tubing, and its end has an acicular pores, and this needle tubing is the opening that is used for inserting this inlet and covers this inlet.
5, the cultivation sampling unit of microorganism according to claim 3, it is characterized in that: this filtrate container is an erlenmeyer flask, this erlenmeyer flask is connected with this discharge outlet by a pipe connecting, the side of this erlenmeyer flask has an opening and is connected with this vacuum suction device, so that form vacuum suction.
6, according to the cultivation sampling unit of the described microorganism of claim 4, it is characterized in that: the bore of this inlet is 4mm, and the bore of this acicular pores is between 1-3mm.
7, the cultivation sampling unit of microorganism according to claim 1, it is characterized in that: this sample liquids is a ultrapure water.
8, the cultivation sampling unit of microorganism according to claim 1, it is characterized in that: this sample liquids is a deionized water.
9, the cultivation sampling unit of microorganism according to claim 3 is characterized in that: this liquid sample is from the sampling sterilizing bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031459404A CN100398636C (en) | 2003-07-17 | 2003-07-17 | Microbial cultivation sampling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031459404A CN100398636C (en) | 2003-07-17 | 2003-07-17 | Microbial cultivation sampling device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1570075A true CN1570075A (en) | 2005-01-26 |
| CN100398636C CN100398636C (en) | 2008-07-02 |
Family
ID=34471561
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB031459404A Expired - Fee Related CN100398636C (en) | 2003-07-17 | 2003-07-17 | Microbial cultivation sampling device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100398636C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101835428B (en) * | 2007-10-23 | 2012-01-04 | 贝克顿·迪金森公司 | Tissue container for molecular and histology diagnostics incorporating a breakable membrane |
| CN102337210A (en) * | 2011-10-10 | 2012-02-01 | 天津开发区合普工贸有限公司 | Positive and negative pressure compatible cell exposure aseptic shielding cabinet equipment |
| CN107096385A (en) * | 2017-06-09 | 2017-08-29 | 天津施特雷生物科技股份有限公司 | A kind of bacteriological filter equipment |
| CN110938518A (en) * | 2019-10-24 | 2020-03-31 | 中山大学 | Filtering device and method for microbial enzyme activity test |
| CN111686827A (en) * | 2019-03-15 | 2020-09-22 | 思耐睿化学产品公司 | System and method for filtering samples from containers |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87208847U (en) * | 1987-06-03 | 1988-04-27 | 浙江宁海县白石药检仪器厂 | Filter |
| CN2147894Y (en) * | 1992-11-19 | 1993-12-01 | 倪卫菊 | Disposable millipore filter |
| CN2169629Y (en) * | 1993-04-24 | 1994-06-22 | 叶大林 | Disposable micro porous filtering and cultivating device |
| CN2479495Y (en) * | 2001-05-15 | 2002-02-27 | 曹惊雷 | Cell harvesting and culturing device |
-
2003
- 2003-07-17 CN CNB031459404A patent/CN100398636C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101835428B (en) * | 2007-10-23 | 2012-01-04 | 贝克顿·迪金森公司 | Tissue container for molecular and histology diagnostics incorporating a breakable membrane |
| CN102337210A (en) * | 2011-10-10 | 2012-02-01 | 天津开发区合普工贸有限公司 | Positive and negative pressure compatible cell exposure aseptic shielding cabinet equipment |
| CN102337210B (en) * | 2011-10-10 | 2013-01-23 | 天津开发区合普工贸有限公司 | Positive and negative pressure compatible cell exposure aseptic shielding cabinet equipment |
| CN107096385A (en) * | 2017-06-09 | 2017-08-29 | 天津施特雷生物科技股份有限公司 | A kind of bacteriological filter equipment |
| CN111686827A (en) * | 2019-03-15 | 2020-09-22 | 思耐睿化学产品公司 | System and method for filtering samples from containers |
| CN110938518A (en) * | 2019-10-24 | 2020-03-31 | 中山大学 | Filtering device and method for microbial enzyme activity test |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100398636C (en) | 2008-07-02 |
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Granted publication date: 20080702 Termination date: 20200717 |
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