TWI329671B - - Google Patents

Description

1329671

FIELD OF THE INVENTION The present invention relates to a microbial culture sampling device for collecting a sample separated by a membrane culture dish: =; heart: to filter and analyze a positive pressure water stream and to analyze the liquid sample to be filtered. Prior art: Thin-film transistors (TFTs) fabrication = ^; step, with the advantages of thin and light 4 ... ray (1 iqu id crystal display; LCD), is widely used The manufacturing technology of various electronic products such as computers, personal digital assistants (PDAs), watches, notebook computers, digital cameras, liquid crystal displays and mobile phones can be mainly divided into: (1) matrix process (Array pr〇) Cess); (2) panel process (panel process^; and (3) module process (M〇duU. In the above-mentioned eve process, 'usually use pure water, such as ultrapure water (upw) ^ deionized water ( DIW) to clean the TFT panel, or when the TFT panel uses a number of chemical agents, it is also necessary to add pure water to dilute the chemical agents. The pure water still contains microorganisms, and the diameter is at least about 1 micron or more. , just like particles. 'If the microbes in pure water stay on the panel,

1329671 V. Description of the invention (2) It will cause short-circuit and disconnection of the panel, which will affect the quality of the process. Therefore, the pure water used in the process must first pass the microbiological content test to determine that the number of microbial bacteria contained in the process is within the allowable range, for example, the number of bacteria contained in each liter of pure water is less than 50. . In the analysis of microbial samples of pure water, the main purpose is to calculate the number of bacteria contained per 100 ml or per liter of pure water. In the pretreatment method for microbial sample analysis, the sample water using the sampling sterilization bag cannot pass the microbial filter because it does not have water pressure, so it is currently analyzed through a specially designed Millipore Milliflex-ΙΟΟ detection system. The pure water is vacuum filtered to leave the bacteria on the filter membrane. After the bacteria culture method, the bacteria on the filter membrane form colonies, and then the number of colonies is calculated, and the pure water sample can be known every 1 〇〇 ml. The number of bacteria contained (a bacterium will form a colony). Figure 1 is a simplified schematic diagram of the Millipore Mi 11 if lex-100 detection system 10, which includes a microbial filter culture dish 11 and a 11 port vacuum pump 12, wherein the microbial filter culture lone 11 is An integrally formed water container having a microbial filter membrane 110 at the bottom. The vacuum pump 12 has two filter stages 120. The diameter of the filter stage 120 must coincide with the diameter of the microbial filter membrane 110. First, the microbial filter membrane 110 of the microbial filter culture chamber 11 is partially inserted into the vacuum pump 12, any one of the filter loading platforms 120, and a waste water container 13 is connected to the waste water outlet of the vacuum pump 12; , the sample water of a sampling sterilization bag is poured into the water container of the micro-production 5, the invention description (3) culture filter culture, and vacuum filtration (formation of a negative covering force) is performed to make the sample The water is pumped out to the waste water container 13 through the microfiltration membrane, and the bacteria in the sample water remain on the filter membrane 1 'after' from the filter substrate 丨2 〇 The microbial filter culture dish 11' is taken out and the filter membrane 110 at the bottom of the microbial filter culture dish u is cut out, and the filter membrane 110 is placed on a substrate disc culture dish (not shown) having a medium injection. An inlet (not shown) was poured from the injection port to culture the bacteria forming colonies on the filter 110 to calculate a fine number. Since the MilliP0re Milliflex_1〇〇 detection system 1〇 is a kit (k i t ), the microbial filter culture dish must be matched with the

The MilliPOre vacuum pump 12 is used together at a high price, and the microbial filter culture dish 11 is discarded after cutting the film at the bottom of the membrane, and can be reused, so that it is not necessary to use the pretreatment step for analyzing the existing microbial sample. The filtration culture system used has been proposed to improve the process cost. SUMMARY OF THE INVENTION One object of the present invention is to provide a sample collector having a repeatable use for filtering a liquid sample having a positive pressure filtration analysis. A microbial culture sampling device is used, and the microbial culture sampling device has a force water flow and a need to provide a negative pressure. 13296671 V. Another aspect of the present invention is to provide a microbial culture sampling device which can be matched with A typical vacuum pumping device that produces vacuum suction at a negative pressure, which is inexpensive, can greatly reduce the cost of microbial analysis. Male The present invention discloses a microbial culture sampling device comprising a sample collector, a culture dish and a filtrate container. The sample collector is configured to collect a liquid sample containing microorganisms, and further comprises a similar injector; the culture dish is configured to filter out microorganisms in the liquid sample on the filter membrane, and the method comprises an injection port, a filter membrane and a discharge port, wherein the injection port cooperates with the sample injector to receive the liquid sample flowing from the sample injector. The filter membrane is disposed between the injection port and the discharge port And the filtrate container is combined with the discharge port to form a relative negative pressure with the sample collector, such that the liquid sample flowing out of the sample injector can enter the inside of the culture dish through the injection port; After passing through the film, the smear discharge port is discharged to the filtrate container, and the microorganism is left in the filter membrane of the culture dish for cultivation. Furthermore, in the microbial culture sampling device of the present invention, the relative negative pressure formed between the filtrate container and the collector can be obtained from the sample by the liquid sample collected in the collector. The injector flows out and forms a positive pressure inside the dish to form 'either or by vacuuming the pomelo gas by connecting a vacuum evacuation device to the filtrate. Implementation method:

1329671

Z ^ ^ a is a schematic view of a microbial culture sampling device 50 of the present invention. The culture sampling device 50 comprises a sample collection, a culture dish 52 and a filtrate container (for example, an argon flask 3). The microbial culture sampling device 5 can be used to filter the positive pressure water flow to be filtered. A liquid sample is analyzed, for example, water released by a faucet; or as shown in FIG. 2, as long as the microorganism culture apparatus 50 is disposed between the filtrate container and the sample collector, a " The vacuum pumping device 54 can generate the vacuum attraction of the negative pressure.

Filtration analysis requires a negative pressure sample to be filtered for analysis, such as sample water in a sample sterilisation bag or water that has flowed into the tank of the process machine.

In the microbial culture sampling device 50, the sample collector 51 includes the same: a storage gauge 511 and a sample injector 5丨2, wherein the sample storage detector 511 is configured to receive a microorganism-containing a liquid sample 40, such as ultrapure water or deionized water, and the sample storage gauge 511 has a scale, γ directly/measures the volume content of the poured liquid sample 40, and the sample injector 5 1 2 The bottom of the sample storage measuring device 5 11 is connected. The sample injector 511 is a needle tube for inserting the injection port and shielding the opening of the injection port. The needle end has a needle hole 5丨3. The caliber is in the range of about 1 to 3 mm. The upper surface of the culture m52 has a protruding injection port 521 for avoiding

1329671

The boundary of the injection port 521 is small, about 4 mm, and the liquid sample flowing out from the needle-shaped hole 51 3 of the sample injector 5 11 is injected into the culture blood 52 through the injection port 521 through the culture dish. The membrane of 52 is filtered to pass through the downward positive pressure of the liquid sample or the negative pressure applied by the vacuum pumping device 54 under the bowl, and the membrane of the petri dish 52 will scoop out the liquid sample. The microorganisms therein are placed thereon, and the filtrate of the liquid sample flows out from the lower surface of the petri dish 52 and is received by the Erlenmeyer flask 53. On the lower surface of the culture dish 52, there is a discharge port 522 which is connected to the discharge port 522 of the lower surface of the culture orphan 52 through a connecting pipe 531 and has an opening on the side of the conical flask 53. 532 is coupled to the vacuum pumping unit 54 for vacuum pumping, and a negative pressure is formed below the petri dish 52 to accelerate the filtration rate of the sample. The sample collector of the microorganism culture sampling device of the present invention can be repeatedly used. When not in use, the sample collector can be immersed in the crucible 202 to be completely sterilized, and when it is used, it is taken out from the crucible 202 and rinsed with pure water. Just fine. Further, the microorganism culture sampling device of the present invention can be used in combination with a general vacuum pumping device. Therefore, the entire microbial culture sampling device is inexpensive, compared to the Mi 11 iPG used by the prior art; re Μί 11 if lex-i0〇 detection system can reduce the cost of microbial analysis of a liquid crystal display manufacturer by about two per year. NT$150,000. Further, the microbial culture sampling sample of the present invention is applicable to the analysis sample to be filtered which has a positive pressure water flow itself and which is required to provide a negative pressure, and the microbiological analysis result is also good.

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For use with ^ σσ , Β β , through the micro-scarring of the present invention, even the strong:: Mo Li water flow and the need for a sampling device, not only the station to reduce the microbial fraction;: i pressure; The cost of research. As described above, the scope of the present invention is utilized. Modifications and adaptations of the present invention will be apparent to those skilled in the art without departing from the scope of the invention. The present invention, however, is not to be construed as limited, the details of the present invention, and the description of the accompanying drawings. Advantages, wherein: Figure 2 is a simplified schematic diagram of a conventional Millip〇re Milliflex-ΙΟΟ detection system; and the same schematic diagram of the microbial culture sampling device of the present invention. Discussion 1 main yoke element diagram description: llipore Milliflex-ΙΟΟ detection system 1 microbial filter culture dish 11 microbial filter 110 wastewater container 1 3 sample collector 51 Erlenmeyer flask 5 3 sample storage measuring device 511 liquid sample 4 0 Note inlet 5 2 1 Connection tube 531

Mi 11 ipore vacuum pump j 2 filter stage 1 2 0 microbial culture sampling device 5 〇 culture blood _52 vacuum suction device 54 sample injector 5 1 2 needle hole 513. Drain 5 2 2 opening 53 2

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Claims (1)

1329671 _ f _ - ♦ 〇 ""丨"丨7^77: ~ J p4^ Sweater (more) original six, f game patent ···· ! . _ 1. One kind of microbial culture, The sampling device comprises: a sample collector for collecting a liquid sample containing microorganisms, further comprising a sample injector; a culture dish comprising an injection port, a filter membrane and a discharge port, 'the injection port cooperates with the sample injector to receive the liquid sample flowing from the sample injector, the filter is disposed between the injection port and the discharge port; and a filtrate container The discharge port is combined to form a relative negative pressure with the sample collector, so that the liquid sample flowing out of the sample injector can enter the culture orphan through the injection port, and the filter passes through the filter. Thereafter, the discharge port is discharged to the filtrate container, and the microorganism is left in the culture filter to be cultured. 2. The microorganism culture sampling device according to claim 1, wherein a relative negative pressure formed between the filtrate container and the sample collector is obtained by collecting a liquid sample collected in the sample collector. Formed with a forward pressure that flows from the sample injector and into the interior of the culture vessel. 3. The microbial culture sampling device according to claim 1, wherein the relative negative pressure formed between the filtrate container and the sample collector is connected to a vacuum pumping device in the filtrate container. It is formed by vacuum pumping. 4. The microorganism culture sampling device according to claim 1, wherein Page 14 13296671 VI. Patent application scope and wherein the sample injector is a needle tube having a needle-shaped needle tube at the end for inserting the injection port and shielding the opening 5 of the injection port. The microorganism culture sampling device according to Item 3, wherein the filtrate container is an Erlenmeyer flask, and the Erlenmeyer flask is connected to the discharge port through a connecting pipe, and the side of the Erlenmeyer flask has an opening and the vacuum The air suction devices are connected to form a vacuum pumping. 6. The microbial culture sampling device according to claim 4, wherein the diameter of the injection port is 4 m, and the diameter of the needle hole is in the range of 1 - 3 。. 7. The microorganism culture sampling device according to claim 1, wherein the liquid sample is ultrapure water. 8. The microorganism culture sampling device according to claim 1, wherein the liquid sample is deionized water. 9. The microbial culture sampling device of claim 3, wherein the liquid sample is from a sampling sterilization bag. Page 15