CN201971837U - Cell radon exposure toxicant contamination experiment device - Google Patents
Cell radon exposure toxicant contamination experiment device Download PDFInfo
- Publication number
- CN201971837U CN201971837U CN 201120084152 CN201120084152U CN201971837U CN 201971837 U CN201971837 U CN 201971837U CN 201120084152 CN201120084152 CN 201120084152 CN 201120084152 U CN201120084152 U CN 201120084152U CN 201971837 U CN201971837 U CN 201971837U
- Authority
- CN
- China
- Prior art keywords
- chamber
- radon
- cell
- gas
- exposure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Abstract
The utility model provides a cell radon exposure toxicant contamination experiment device which comprises an air compression air source, a radon measuring meter, an accumulation chamber, an explosion flow adjusting controller, a radon exhaustion flow controller and a waste gas treatment device. The air compression air source respectively supplies air to the accumulation chamber and a cell exposure chamber; the radon source is connected to the accumulation chamber by a source filling loop for providing the radon; the radon measuring meter is used for monitoring the content of the measured radon; the accumulation chamber is communicated to the cell exposure chamber; the explosion flow adjusting controller and the radon exhaustion flow controller can adjust the flow of the gas in the cell exposure chamber; the waste gas passes through an air collection port to securely exhausts through the waste gas treatment device. A cell membrane plate is placed in the cell exposure chamber, exposure toxicant contamination is carried out for the upper surface while the lower surface is soaked in a cell culture liquid chamber; the cell exposure chamber and the cell culture liquid chamber are arranged in an incubation chamber; and a sterilization gas supply device is connected to all gas loops and cell exposure chambers for sterilizing. The cell radon exposure toxicant contamination experiment device has the advantages that the cell radon exposure toxicant contamination experiment device with unavailability of experiment animals, small radon consumption quantity, security, accuracy, convenience and reliability is provided, so that new method and equipment are provided for scientific research to fill the gap of the aspect.
Description
Technical field
The utility model relates to a kind of cell radon gas and exposes exposure experimental device.
Background technology
Natural radon gas is ubiquitous, and the pollution of radon gas and daughter thereof is the major incentive that causes diseases such as Human Lung Cancer, is subjected to the attention of scientific circles deeply in fields such as toxicity, environmental protection, molecular biosciences, cell, radiation, medical science.Carry out the scientific research of this respect in the past adopts the method that exposes experiment in the animal body to verify more.It is big to have required equipment volume, and size of animal is many, and consumption radon gas amount and pollution are many, and the experimental result error is big, is subject to shortcomings such as interference.Seriously restricted the scientific research development of this respect.
Summary of the invention
The purpose of this utility model provides a kind of cell radon gas and exposes exposure experimental device, for relevant scientific research provide a kind of volume little, without laboratory animal, consume that radon is narrow-minded, safety do not have leak, accurate quantification control radon gas and daughter content thereof, accurate, easy to operate, the reliable animal body of experimental result is outer and the cellular exposure exposure experimental device, for providing new method and apparatus, filled up blank in this respect in field scientific researches in this respect such as toxicity, environmental protection, molecular biosciences, cell, radiation, medical science.
For achieving the above object, the technical solution of the utility model provides a kind of cell radon gas exposure exposure experimental device.This device includes: the radon need that radon gas can take place connects fills resource loop to accumulating chamber, fills the source recycle pump and fills source flux controller adjustment accumulating chamber radon gas content; There is the emanometer table to monitor radon gas content in the accumulating chamber by surveying the radon loop; By exposed circuit, exposure cycle pump with expose the radon gas flow director and carry the mixed gas of the radon gas of predetermined content and corresponding daughter in one or several indoor cellular exposure chambeies of incubation; The air compressed gas source provide fresh air can be respectively for accumulating chamber and each cellular exposure chamber, adjusts the radon gas in accumulating chamber and each cellular exposure chamber and the mixed gas content of daughter thereof respectively by regulating the accumulation air flow controller and exposing flow director; Several cell lamina membranaceas of having planted experimental cell are placed in separately the cellular exposure chamber, each exposes the diffusion gas port that a downward bell mouth shape is arranged in chamber and aims at each cell lamina membranacea upper surface and carry out sufficient radon gas and expose contamination, mixed gas in each cellular exposure chamber reclaims through collecting gas port, by the discharge pipe and the emission-control equipment safety dumping of band emission flow controller.Each cell lamina membranacea lower surface soaks into and guarantee the normal nutrition supply of cell in the cell cultures sap cavity that connects the cell cultures liquid supply unit; It is indoor that each cellular exposure chamber and cell cultures sap cavity are installed in same incubation, and the incubation control device that is connected with the incubation chamber guarantees that the environment of each cellular exposure chamber and cell cultures sap cavity is constant in being beneficial to the existence temperature of cell.Special-purpose sterilizing gas feeding mechanism is connected to and can allows the full and stream of sterilizing gas carry out the thorough disinfection sterilization by all gas loop and each cellular exposure chamber in the output loop of air compressed gas source.
The beneficial effects of the utility model provide a kind of volume little, without laboratory animal, consume that radon is narrow-minded, safety do not have leak, accurate quantification control radon gas and daughter content thereof, accurate, easy to operate, the reliable animal body of experimental result is outer and the cellular exposure exposure experimental device, for field scientific researches in this respect such as toxicity, environmental protection, molecular biosciences, cell, radiation, medical science provide new method and apparatus, filled up blank in this respect.
Description of drawings
Accompanying drawing is the synoptic diagram of the utility model structure;
Among the figure:
1, air compressed gas source
2, accumulation air flow controller
3, expose air flow controller
4, radon need
5, fill the source recycle pump
6, fill the source flux controller
7, emanometer table
8, survey the radon loop
9, accumulating chamber
10, exposure cycle pump
11, expose the radon gas flow director
12, cellular exposure chamber
13, cell cultures sap cavity
14, diffusion gas port
15, collect gas port
16, cell lamina membranacea
17, cell cultures liquid supply unit
18, incubation control device
19, expose flow director
20, radon gas emission flow controller
21, emission-control equipment
22, emission flow controller
23, fill resource loop
24, exposed circuit
25, discharge pipe
26, incubation chamber
27, sterilizing gas feeding mechanism.
Embodiment
Reaching embodiment in conjunction with the accompanying drawings is illustrated cell radon gas exposure exposure experimental device of the present utility model.
As shown in drawings, the utility model device includes: the radon need 4 that radon gas can take place connects fills resource loop 23 to accumulating chamber 9, fills source recycle pump 5 and fills source flux controller 6 and can adjust radon gas and daughter accumulation volume thereof in the accumulating chamber 9; Air compressed gas source 1 can provide fresh air for respectively accumulating chamber 9 and each cellular exposure chamber 12.Can regulate the radon gas content in the accumulating chamber 9 and monitor by surveying radon loop 8 by regulating accumulation air flow controller 2 and filling source flux controller 6 with emanometer table 7; By exposed circuit 24, exposure cycle pump 10 with expose radon gas flow director 11 in mixed gas that diffusion gas port 14 is carried the radon gas of predetermined contents and corresponding daughter one or several cellular exposure chambeies 12 in the incubation chamber 26; Regulate exposure flow director 19 and radon gas emission flow controller 20 in the experimentation and can adjust the flow of the mixed gas in each cellular exposure chamber 12; Several cell lamina membranaceas 16 of having planted experimental cell are placed in separately the cellular exposure chamber 12, there are diffusion gas port 14 each cell lamina membranacea 16 upper surface of aligning of a downward bell mouth shape to carry out sufficient radon gas exposure contamination in each cellular exposure chamber 12, mixed gas in each cellular exposure chamber 12 reclaims through collecting gas port 15, by the discharge pipe 25 and emission-control equipment 21 safety dumpings of band emission flow controller 22.Each cell lamina membranacea 16 lower surface soaks in cell cultures sap cavity 13, has the cell culture fluid that is provided by the cell cultures liquid supply unit 17 that is connected can guarantee the cell normal existence in the cell cultures sap cavity 13; Each cellular exposure chamber 12 and cell cultures sap cavity 13 are installed in the incubation chamber 26 that is connected to incubation control device 18, guarantee that the environment of each cellular exposure chamber 12 and cell cultures sap cavity 13 is constant in the temperature of the existence that helps cell.The output loop that special-purpose sterilizing gas feeding mechanism 27 is connected to air compressed gas source 1 can allow the full and stream of sterilizing gas carry out the thorough disinfection sterilization by all gas loop and each cellular exposure chamber 12.
Embodiment:
When using the cell radon gas to expose exposure experimental device, at first close radon need 4 and air compressor 1, open all flow directors and exposure cycle pump 10, unlatching sterilizing gas feeding mechanism 27 allows sterilizing gas fill and stream carries out the thorough disinfection sterilization by all gas loop and each cellular exposure chamber 12.Close all flow directors and exposure cycle pump 10 after the sterilization immediately, start incubation control device 18, open several cell lamina membranaceas 16 that will plant experimental cell in incubation chamber 26 under aseptic condition and be placed in separately the cellular exposure chamber 12,26 backs, closed incubation chamber are opened cell cultures liquid supply units 17 and radon need 4 and air compressor 1 and are regulated and expose air flow controller 2 and fill radon gas that source flux controller 6 prepares accumulating chamber 9 to regulate predetermined content and the mixed gas of corresponding daughter; Open and expose radon gas flow director 11 and exposure cycle pump 10 and drive mixed gass and be delivered in each cellular exposure chamber 12 through diffusion gas port 14, the mixed gas flow that adjusting exposure flow director 19 and radon gas emission flow controller 20 expose in the chamber 12 thin each born of the same parents is constant, and the upper surface that the radon gas of predetermined content and the mixed gas of corresponding daughter are evenly distributed on each cell lamina membranacea 16 carries out the radon gas exposure experiment to experimental cell; Exposed circuit 24 can be sent the mixed gas of the radon gas that reclaims and daughter thereof back to accumulating chamber 9 and be accumulated once more and recycle.After experiment finishes, close radon need 4, fill source recycle pump 5, fill source flux controller 6, exposure cycle pump 10, expose radon gas flow director 11 and accumulation air flow controller 2; Open and expose air flow controller 3 and emission flow controller 22, purge with fresh air and to remain in each cellular exposure chamber 12, cell lamina membranacea 16, diffusion gas port 14, collect the waste gas such as mixed gas in gas port 15 and the respective line thereof, waste gas is delivered to emission-control equipment 21 and is discharged through the pollution-free back of complete adsorption treatment through exposing air flow controller 3 and discharge pipe 25.After purging removing fully, the flow director of closing air compressed gas source 1 and each loop; Open incubation chamber 26 and under aseptic condition, take out each the cell lamina membranacea 16 that has experimental cell data analysis that experimentizes; Close cell cultures liquid supply unit 17 and incubation control device 18 at last.
Claims (6)
1. a cell radon gas exposes exposure experimental device, it is characterized in that: the radon need (4) that radon gas can take place connects fills resource loop (23) to accumulating chamber (9), fill source recycle pump (5) and fill source flux controller (6) and can adjust the radon gas in the accumulating chamber (9) and the accumulation volume of daughter thereof, air compressed gas source (1) provides fresh air can for accumulating chamber (9), by regulating accumulation air flow controller (2) and filling source flux controller (6) and can regulate the radon gas content in the accumulating chamber (9) and monitor by survey radon loop (8) with emanometer table (7), by exposed circuit (24), exposure cycle pump (10) and expose radon gas flow director (11) in mixed gas that diffusion gas port (14) is carried the radon gas of predetermined content and corresponding daughter one or several cellular exposure chambeies (12) in incubation chamber (26), regulate exposure flow director (19) and radon gas emission flow controller (20) and can adjust the flow of the mixed gas in each cellular exposure chamber (12), mixed gas in each cellular exposure chamber (12) reclaims through collecting gas port (15), discharges by the discharge pipe (25) and the emission-control equipment (21) of band emission flow controller (22).
2. cell radon gas according to claim 1 exposes exposure experimental device, it is characterized in that: incubation chamber (26) are cellular exposure chamber (12) that holds cell lamina membranacea (16) and the cell cultures sap cavity (13) that has several to keep apart in the two-part heat conduction hollow body up and down that can be fixed together, be that wear ring guarantees resistance to air loss in the middle of the cell cultures sap cavity (13) of the cellular exposure chamber (12) of top and bottom, in the middle of cell lamina membranacea (16) is fixed on, having the diffusing opening (14) of a downward bell mouth shape to aim at each cell lamina membranacea (16) upper surface in each cellular exposure chamber (12) carries out sufficient radon gas and exposes contamination, each cell lamina membranacea (16) lower surface soaks in cell cultures sap cavity (13), there is the cell culture fluid that provides by the cell cultures liquid supply unit (17) that is connected to guarantee the cell normal existence in the cell cultures sap cavity (13), each cellular exposure chamber (12) guarantees that with in cell cultures sap cavity (13) is installed in the incubation chamber (26) that is connected incubation control device (18) environment of each cellular exposure chamber (12) and cell cultures sap cavity (13) is constant in helping the temperature of cells survival.
3. cell radon gas according to claim 1 exposes exposure experimental device, it is characterized in that: have special-purpose sterilizing gas feeding mechanism (27) that output loop that ozone or other sterilizing gas be connected to air compressed gas source (1) through pressurization can take place and enter various piece in the exposure system, can allow the full and stream of sterilizing gas carry out the thorough disinfection sterilization, close own valve switch after the sterilization end and isolate from exposure system by all gas loop and each cellular exposure chamber (12).
4. cell radon gas according to claim 1 exposes exposure experimental device, it is characterized in that: the radon need (4) that radon gas can take place is an airtight container that has inlet pipe and escape pipe that radio active material is housed, fill source recycle pump (5) and fill gas stream that source flux controller (6) drives certain flow rate and cross radon need (4) and can drive radon gas and the daughter thereof that radio active material produces and flow into accumulating chamber (9), regulate ozone that air compressed gas source (1) is transported to accumulating chamber (9) by pipeline and can control radon gas and daughter content thereof in accumulating chamber (9) and cellular exposure chamber (12).
5. cell radon gas according to claim 1 exposes exposure experimental device, it is characterized in that: air compressed gas source (1) can provide fresh air directly for each cellular exposure chamber (12) by exposing air flow controller (3), thereby further diluting cells exposes radon gas and the daughter content thereof in the chamber (12), purges and removes each cellular exposure chamber (12), diffusion gas port (14), collects the waste gas of gas port (15) and exposed circuit (24).
6. cell radon gas according to claim 1 exposes exposure experimental device, it is characterized in that: exposed circuit (24) has exposure cycle pump (10), and several diffusion gas ports (14) in incubation chamber (26) are transported to and carry out the cell radon gas in the cellular exposure chamber (12) and expose exposure experiment with the radon gas of the interior predetermined content of accumulating chamber (9) and daughter thereof, there is collection gas port (15) inside, cellular exposure chamber (12), collecting cell exposes the experiment waste gas in the chamber (12) respectively, discharge to emission-control equipment (21) adsorption treatment through discharge pipe (25), its emission-control equipment (21) is the airtight cabinet of being made up of filtering adsorbing material of a band negative pressure-pumping.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120084152 CN201971837U (en) | 2011-03-28 | 2011-03-28 | Cell radon exposure toxicant contamination experiment device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120084152 CN201971837U (en) | 2011-03-28 | 2011-03-28 | Cell radon exposure toxicant contamination experiment device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201971837U true CN201971837U (en) | 2011-09-14 |
Family
ID=44576944
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120084152 Expired - Lifetime CN201971837U (en) | 2011-03-28 | 2011-03-28 | Cell radon exposure toxicant contamination experiment device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201971837U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102191177A (en) * | 2011-03-28 | 2011-09-21 | 天津开发区合普工贸有限公司 | Cell radon exposure experimental device |
| CN102337210A (en) * | 2011-10-10 | 2012-02-01 | 天津开发区合普工贸有限公司 | Positive and negative pressure compatible cell exposure aseptic shielding cabinet equipment |
| CN111849773A (en) * | 2020-08-11 | 2020-10-30 | 中国工程物理研究院核物理与化学研究所 | Cell tritium water irradiation experimental device |
-
2011
- 2011-03-28 CN CN 201120084152 patent/CN201971837U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102191177A (en) * | 2011-03-28 | 2011-09-21 | 天津开发区合普工贸有限公司 | Cell radon exposure experimental device |
| CN102337210A (en) * | 2011-10-10 | 2012-02-01 | 天津开发区合普工贸有限公司 | Positive and negative pressure compatible cell exposure aseptic shielding cabinet equipment |
| CN102337210B (en) * | 2011-10-10 | 2013-01-23 | 天津开发区合普工贸有限公司 | Positive and negative pressure compatible cell exposure aseptic shielding cabinet equipment |
| CN111849773A (en) * | 2020-08-11 | 2020-10-30 | 中国工程物理研究院核物理与化学研究所 | Cell tritium water irradiation experimental device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN201971837U (en) | Cell radon exposure toxicant contamination experiment device | |
| CN100497583C (en) | Safety high-efficient continuous enclosed type cell culture and virus production-inactivation system | |
| CN102796667B (en) | Microfluidic chip allowing different cell-density arrangement and use thereof | |
| CN106442066B (en) | Soil culture device with small gas collector | |
| CN103698330B (en) | A kind of experimental provision and method thereof measuring plant respiration | |
| CN107287120A (en) | A kind of cell prepares feeding system | |
| CN102191176B (en) | Experimental device for cell exposure contamination with combination of radon gas and cigarette smoke | |
| CN102191177B (en) | Cell radon exposure poisoning experimental device | |
| CN201971836U (en) | Cell exposure contamination experimental apparatus with combination of radon gas and cigarette smoke | |
| CN104109631A (en) | Microalgae culture reactor | |
| CN201842846U (en) | Control device of cell exposure gas with controllable concentration | |
| CN201996686U (en) | Radon gas exposure contamination experiment device | |
| CN104226224B (en) | A kind of photocatalysis hydrogen production system and hydrogen production process | |
| JP2012044969A (en) | Method for producing euglena containing no carbon isotope 14c, euglena containing no carbon isotope 14c, method for raising laboratory animal containing no carbon isotope 14c, laboratory animal containing no carbon isotope 14c | |
| CN108865648A (en) | The cell detecting system of major air pollutants ozone toxicity | |
| CN206020212U (en) | A kind of BOD online auto monitoring systems | |
| CN207130276U (en) | A kind of cell prepares feeding system | |
| CN102061258A (en) | Cell exposed anaerobic environment experiment device | |
| WO2003074665A2 (en) | Microcapillary bioreactor for growth of biological tissue | |
| CN215066407U (en) | Special metabolism cage of experimental animals exhaled breath on-line analysis | |
| JP6199098B2 (en) | Method for culturing photosynthetic microorganism not containing carbon isotope 14C | |
| CN220715889U (en) | A well universe climate chamber for environmental mercury research | |
| NL2030379B1 (en) | Novel aeration zone leaching experimental device | |
| CN212321614U (en) | Novel peritracheal band eluviation experiment device | |
| CN201942682U (en) | Cell exposure dust contamination experimental device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20110914 Effective date of abandoning: 20130424 |
|
| RGAV | Abandon patent right to avoid regrant |