CN102191177A - Cell radon exposure experimental device - Google Patents
Cell radon exposure experimental device Download PDFInfo
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- CN102191177A CN102191177A CN2011100744145A CN201110074414A CN102191177A CN 102191177 A CN102191177 A CN 102191177A CN 2011100744145 A CN2011100744145 A CN 2011100744145A CN 201110074414 A CN201110074414 A CN 201110074414A CN 102191177 A CN102191177 A CN 102191177A
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Abstract
The invention provides a cell radon exposure experimental device. The device comprises the following structures: an air-compressing gas source provides air for an accumulation chamber and a cell exposure cavity respectively, a radon source is connected to the accumulation chamber through an input loop to provide radon, a radon-measuring instrument is used to monitor the content of radon; the accumulation chamber is communicated with the cell exposure cavity; an exposure flow controller and a radon discharge flow controller are adjusted to adjust the flow of the gas in the cell exposure cavity; and the waste gas passes through a gas-collecting hole and is discharged safely by a waste gas treatment device. A cell film plate is placed in the cell exposure cavity, the upper surface is exposed, the lower surface is soaked in a cell culture solution cavity, the cell exposure cavity and the cell culture solution cavity are arranged in an incubation chamber, and a sterile gas supply device is connected with all the gas loops and the cell exposure cavity for disinfection and sterilization. The invention has the following beneficial effects: the invention provides a cell radon exposure experimental device which does use laboratory animal, has low radon consumption and is safe, accurate, convenient and reliable, provides the new method and device for scientific research and fills in the blank of the aspect.
Description
Technical field
The present invention relates to a kind of cell radon gas and expose exposure experiment equipment.
Background technology
Natural radon gas is ubiquitous, and the pollution of radon gas and daughter thereof is the major incentive that causes diseases such as Human Lung Cancer, is subjected to the attention of scientific circles deeply in fields such as toxicity, environmental protection, molecular biosciences, cell, radiation, medical science.Carry out the scientific research of this respect in the past adopts the method that exposes experiment in the animal body to verify more.It is big to have required equipment volume, and size of animal is many, and consumption radon gas amount and pollution are many, and the experimental result error is big, is subject to shortcomings such as interference.Seriously restricted the scientific research development of this respect.
Summary of the invention
The purpose of this invention is to provide a kind of cell radon gas and expose exposure experiment equipment, for relevant scientific research provide a kind of volume little, need not laboratory animal, consume that radon is narrow-minded, safety do not have leak, accurate quantification control radon gas and daughter content thereof, accurate, easy to operate, the reliable animal body of experimental result is outer and cellular exposure exposure experiment equipment, for providing new method and apparatus, filled up blank in this respect in field scientific researches in this respect such as toxicity, environmental protection, molecular biosciences, cell, radiation, medical science.
For achieving the above object, technical scheme of the present invention provides a kind of cell radon gas exposure exposure experiment equipment.This equipment includes: the radon need that radon gas can take place connects fills resource loop to accumulating chamber, fills the source recycle pump and fills source flux controller adjustment accumulating chamber radon gas content; There is the emanometer table to monitor radon gas content in the accumulating chamber by surveying the radon loop; By exposed circuit, exposure cycle pump with expose the radon gas flow director and carry the mixed gas of the radon gas of predetermined content and corresponding daughter in one or several indoor cellular exposure chambeies of incubation; The air compressed gas source provide fresh air can be respectively for accumulating chamber and each cellular exposure chamber, adjusts the radon gas in accumulating chamber and each cellular exposure chamber and the mixed gas content of daughter thereof respectively by regulating the accumulation air flow controller and exposing flow director; Several cell lamina membranaceas of having planted experimental cell are placed in separately the cellular exposure chamber, each exposes the diffusion gas port that a downward bell mouth shape is arranged in chamber and aims at each cell lamina membranacea upper surface and carry out sufficient radon gas and expose contamination, mixed gas in each cellular exposure chamber reclaims through collecting gas port, by the discharge pipe and the waste gas treatment equipment safety dumping of band emission flow controller.Each cell lamina membranacea lower surface soaks into and guarantee the normal nutrition supply of cell in the cell cultures sap cavity that connects the cell culture fluid supply equipment; It is indoor that each cellular exposure chamber and cell cultures sap cavity are installed in same incubation, and the incubation operating device that is connected with the incubation chamber guarantees that the environment of each cellular exposure chamber and cell cultures sap cavity is constant in being beneficial to the existence temperature of cell.Special-purpose sterilizing gas supply equipment is connected to and can allows the full and stream of sterilizing gas carry out the thorough disinfection sterilization by all gas loop and each cellular exposure chamber in the output loop of air compressed gas source.
The invention has the beneficial effects as follows provide a kind of volume little, need not laboratory animal, consume that radon is narrow-minded, safety do not have leak, accurate quantification control radon gas and daughter content thereof, accurate, easy to operate, the reliable animal body of experimental result is outer and cellular exposure exposure experiment equipment, for field scientific researches in this respect such as toxicity, environmental protection, molecular biosciences, cell, radiation, medical science provide new method and apparatus, filled up blank in this respect.
Description of drawings
Accompanying drawing is the synoptic diagram of structure of the present invention;
Among the figure:
1, air compressed gas source
2, accumulation air flow controller
3, expose air flow controller
4, radon need
5, fill the source recycle pump
6, fill the source flux controller
7, emanometer table
8, survey the radon loop
9, accumulating chamber
10, exposure cycle pump
11, expose the radon gas flow director
12, cellular exposure chamber
13, cell cultures sap cavity
14, diffusion gas port
15, collect gas port
16, cell lamina membranacea
17, cell cultures liquid supply unit
18, incubation control device
19, expose flow director
20, radon gas emission flow controller
21, emission-control equipment
22, emission flow controller
23, fill resource loop
24, exposed circuit
25, discharge pipe
26, incubation chamber
27, sterilizing gas feeding mechanism.
Embodiment
Reaching embodiment in conjunction with the accompanying drawings is illustrated cell radon gas exposure exposure experiment equipment of the present invention.
As shown in drawings, present device includes: the radon need 4 that radon gas can take place connects fills resource loop 23 to accumulating chamber 9, fills source recycle pump 5 and fills source flux controller 6 and can adjust radon gas and daughter accumulation volume thereof in the accumulating chamber 9; Air compressed gas source 1 can provide fresh air for respectively accumulating chamber 9 and each cellular exposure chamber 12.Can regulate the radon gas content in the accumulating chamber 9 and monitor by surveying radon loop 8 by regulating accumulation air flow controller 2 and filling source flux controller 6 with emanometer table 7; By exposed circuit 24, exposure cycle pump 10 with expose radon gas flow director 11 in mixed gas that diffusion gas port 14 is carried the radon gas of predetermined contents and corresponding daughter one or several cellular exposure chambeies 12 in the incubation chamber 26; Regulate exposure flow director 19 and radon gas emission flow controller 20 in the experimentation and can adjust the flow of the mixed gas in each cellular exposure chamber 12; Several cell lamina membranaceas 16 of having planted experimental cell are placed in separately the cellular exposure chamber 12, there are diffusion gas port 14 each cell lamina membranacea 16 upper surface of aligning of a downward bell mouth shape to carry out sufficient radon gas exposure contamination in each cellular exposure chamber 12, mixed gas in each cellular exposure chamber 12 reclaims through collecting gas port 15, by the discharge pipe 25 and emission-control equipment 21 safety dumpings of band emission flow controller 22.Each cell lamina membranacea 16 lower surface soaks in cell cultures sap cavity 13, has the cell culture fluid that is provided by the cell cultures liquid supply unit 17 that is connected can guarantee the cell normal existence in the cell cultures sap cavity 13; Each cellular exposure chamber 12 and cell cultures sap cavity 13 are installed in the incubation chamber 26 that is connected to incubation control device 18, guarantee that the environment of each cellular exposure chamber 12 and cell cultures sap cavity 13 is constant in the temperature of the existence that helps cell.The output loop that special-purpose sterilizing gas feeding mechanism 27 is connected to air compressed gas source 1 can allow the full and stream of sterilizing gas carry out the thorough disinfection sterilization by all gas loop and each cellular exposure chamber 12.
Embodiment:
When using the cell radon gas to expose exposure experiment equipment, at first close radon need 4 and air compressor 1, open all flow directors and exposure cycle pump 10, unlatching sterilizing gas feeding mechanism 27 allows sterilizing gas fill and stream carries out the thorough disinfection sterilization by all gas loop and each cellular exposure chamber 12.Close all flow directors and exposure cycle pump 10 after the sterilization immediately, start incubation control device 18, open several cell lamina membranaceas 16 that will plant experimental cell in incubation chamber 26 under aseptic condition and be placed in separately the cellular exposure chamber 12,26 backs, closed incubation chamber are opened cell cultures liquid supply units 17 and radon need 4 and air compressor 1 and are regulated and expose air flow controller 2 and fill radon gas that source flux controller 6 prepares accumulating chamber 9 to regulate predetermined content and the mixed gas of corresponding daughter; Open and expose radon gas flow director 11 and exposure cycle pump 10 and drive mixed gass and be delivered in each cellular exposure chamber 12 through diffusion gas port 14, the mixed gas flow that adjusting exposure flow director 19 and radon gas emission flow controller 20 expose in the chamber 12 thin each born of the same parents is constant, and the upper surface that the radon gas of predetermined content and the mixed gas of corresponding daughter are evenly distributed on each cell lamina membranacea 16 carries out the radon gas exposure experiment to experimental cell; Exposed circuit 24 can be sent the mixed gas of the radon gas that reclaims and daughter thereof back to accumulating chamber 9 and be accumulated once more and recycle.After experiment finishes, close radon need 4, fill source recycle pump 5, fill source flux controller 6, exposure cycle pump 10, expose radon gas flow director 11 and accumulation air flow controller 2; Open and expose air flow controller 3 and emission flow controller 22, purge with fresh air and to remain in each cellular exposure chamber 12, cell lamina membranacea 16, diffusion gas port 14, collect the waste gas such as mixed gas in gas port 15 and the respective line thereof, waste gas is delivered to emission-control equipment 21 and is discharged through the pollution-free back of complete adsorption treatment through exposing air flow controller 3 and discharge pipe 25.After purging removing fully, the flow director of closing air compressed gas source 1 and each loop; Open incubation chamber 26 and under aseptic condition, take out each the cell lamina membranacea 16 that has experimental cell data analysis that experimentizes; Close cell cultures liquid supply unit 17 and incubation control device 18 at last.
Claims (6)
1. a cell radon gas exposes exposure experiment equipment, it is characterized in that: the radon need (4) that radon gas can take place connects fills resource loop (23) to accumulating chamber (9), fill source recycle pump (5) and fill source flux controller (6) and can adjust the radon gas in the accumulating chamber (9) and the accumulation volume of daughter thereof, air compressed gas source (1) provides fresh air can for accumulating chamber (9), by regulating accumulation air flow controller (2) and filling source flux controller (6) and can regulate the radon gas content in the accumulating chamber (9) and monitor by survey radon loop (8) with emanometer table (7); By exposed circuit (24), exposure cycle pump (10) and expose radon gas flow director (11) in mixed gas that diffusion gas port (14) is carried the radon gas of predetermined content and corresponding daughter one or several cellular exposure chambeies (12) in incubation chamber (26), regulate exposure flow director (19) and radon gas emission flow controller (20) and can adjust the flow of the mixed gas in each cellular exposure chamber (12), mixed gas in each cellular exposure chamber (12) reclaims through collecting gas port (15), discharges by the discharge pipe (25) and the emission-control equipment (21) of band emission flow controller (22).
2. cell radon gas according to claim 1 exposes exposure experiment equipment, it is characterized in that: incubation chamber (26) are cellular exposure chamber (12) that holds cell lamina membranacea (16) and the cell cultures sap cavity (13) that has several to keep apart in the two-part heat conduction hollow body up and down that can be fixed together, be that wear ring guarantees resistance to air loss in the middle of the cell cultures sap cavity (13) of the cellular exposure chamber (12) of top and bottom, in the middle of cell lamina membranacea (16) is fixed on, having the diffusing opening (14) of a downward bell mouth shape to aim at each cell lamina membranacea (16) upper surface in each cellular exposure chamber (12) carries out sufficient radon gas and exposes contamination, each cell lamina membranacea (16) lower surface soaks in cell cultures sap cavity (13), there is the cell culture fluid that provides by the cell cultures liquid supply unit (17) that is connected to guarantee the cell normal existence in the cell cultures sap cavity (13), each cellular exposure chamber (12) guarantees that with in cell cultures sap cavity (13) is installed in the incubation chamber (26) that is connected incubation control device (18) environment of each cellular exposure chamber (12) and cell cultures sap cavity (13) is constant in helping the temperature of cells survival.
3. cell radon gas according to claim 1 exposes exposure experiment equipment, it is characterized in that: have special-purpose sterilizing gas feeding mechanism (27) that output loop that ozone or other sterilizing gas be connected to air compressed gas source (1) through pressurization can take place and enter various piece in the exposure system, can allow the full and stream of sterilizing gas carry out the thorough disinfection sterilization, close own valve switch after the sterilization end and isolate from exposure system by all gas loop and each cellular exposure chamber (12).
4. cell radon gas according to claim 1 exposes exposure experiment equipment, it is characterized in that: the radon need (4) that radon gas can take place is an airtight container that has inlet pipe and escape pipe that radio active material is housed, fill source recycle pump (5) and fill gas stream that source flux controller (6) drives certain flow rate and cross radon need (4) and can drive radon gas and the daughter thereof that radio active material produces and flow into accumulating chamber (9), regulate ozone that air compressed gas source (1) is transported to accumulating chamber (9) by pipeline and can control radon gas and daughter content thereof in accumulating chamber (9) and cellular exposure chamber (12).
5. cell radon gas according to claim 1 exposes exposure experiment equipment, it is characterized in that: air compressed gas source 1 can provide fresh air directly for each cellular exposure chamber (12) by exposing air flow controller (3), thereby further diluting cells exposes radon gas and the daughter content thereof in the chamber (12), purges and removes each cellular exposure chamber (12), diffusion gas port (14), collects the waste gas of gas port (15) and exposed circuit (24).
6. cell radon gas according to claim 1 exposes exposure experiment equipment, it is characterized in that: exposed circuit (24) has exposure cycle pump (10) and through incubation chamber (26) in several of the radon gas of interior the predetermined content of accumulating chamber (9) and daughter thereof are spread gas ports (14) is transported to and carries out cell radon gas exposure exposure experiment in the cellular exposure chamber (12); There is collection gas port (15) inside, cellular exposure chamber (12), collecting cell exposes the experiment waste gas in the chamber (12) respectively, discharge to emission-control equipment (21) adsorption treatment through discharge pipe (25), its emission-control equipment (21) is the airtight cabinet of being made up of filtering adsorbing material of band vacuum suction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110074414 CN102191177B (en) | 2011-03-28 | 2011-03-28 | Cell radon exposure poisoning experimental device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110074414 CN102191177B (en) | 2011-03-28 | 2011-03-28 | Cell radon exposure poisoning experimental device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102191177A true CN102191177A (en) | 2011-09-21 |
| CN102191177B CN102191177B (en) | 2013-04-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110074414 Expired - Fee Related CN102191177B (en) | 2011-03-28 | 2011-03-28 | Cell radon exposure poisoning experimental device |
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| CN (1) | CN102191177B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113768657A (en) * | 2021-10-12 | 2021-12-10 | 兵器工业卫生研究所 | Gas inhalation contamination experiment exposure device |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19526533A1 (en) * | 1995-07-20 | 1997-01-23 | Fraunhofer Ges Forschung | Nutritional medium delivered to cell culture through permeable membrane |
| CN201971837U (en) * | 2011-03-28 | 2011-09-14 | 天津开发区合普工贸有限公司 | Cell radon exposure toxicant contamination experiment device |
-
2011
- 2011-03-28 CN CN 201110074414 patent/CN102191177B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19526533A1 (en) * | 1995-07-20 | 1997-01-23 | Fraunhofer Ges Forschung | Nutritional medium delivered to cell culture through permeable membrane |
| CN201971837U (en) * | 2011-03-28 | 2011-09-14 | 天津开发区合普工贸有限公司 | Cell radon exposure toxicant contamination experiment device |
Non-Patent Citations (2)
| Title |
|---|
| M. AUFDERHEIDE等: "A method for in vitro analysis of the biological activityof complex mixtures such as sidestream cigarette smoke", 《EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY》 * |
| MICHAELA AUFDERHEIDE等: "An improved in vitro model for testing the pulmonary toxicity of complex mixtures such as cigarette smoke", 《EXP TOXIC PATHOL》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113768657A (en) * | 2021-10-12 | 2021-12-10 | 兵器工业卫生研究所 | Gas inhalation contamination experiment exposure device |
| CN113768657B (en) * | 2021-10-12 | 2024-05-10 | 兵器工业卫生研究所 | Exposure device for gas inhalation contamination experiment |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102191177B (en) | 2013-04-24 |
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