CN202322846U - Positive-negative pressure compatible cell exposure sterility shielding cabinet device - Google Patents
Positive-negative pressure compatible cell exposure sterility shielding cabinet device Download PDFInfo
- Publication number
- CN202322846U CN202322846U CN 201120382278 CN201120382278U CN202322846U CN 202322846 U CN202322846 U CN 202322846U CN 201120382278 CN201120382278 CN 201120382278 CN 201120382278 U CN201120382278 U CN 201120382278U CN 202322846 U CN202322846 U CN 202322846U
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- Prior art keywords
- negative pressure
- cabinet
- positive
- shielding cabinet
- shielding
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
Abstract
The utility model provides a positive-negative pressure compatible cell exposure sterility shielding cabinet device which comprises a positive pressure dust-free sterile operating cabinet and a negative pressure shielding cabinet. An ultraviolet germicidal lamp and a gas fumigation and ozone sterilizer are arranged inside the positive pressure operating cabinet, and the positive pressure operating cabinet provided with an efficient filtering air supply system is used for performing cell exposure sterility contamination experiment after sterilization. The anti-pollution negative pressure shielding cabinet for aerosol generation is provided with a suction effect and used for performing the aerosol generation, quantitative transmission and exhaust discharge of contamination substance. The two cabinets are closely connected with each other and communicated through a pipeline and a valve. A moving separating board which can be opened and closed is installed on the lower portion of the shielding cabinet device, and glass shielding windows which can be opened or closed up and down in a slide mode are arranged at the front of the two cabinets. The positive-negative pressure compatible cell exposure sterility shielding cabinet device has the advantages that in a combined cabinet, the negative pressure cabinet is used for sucking air inward to protect operation staff and to complete operation of toxic substance on one hand, the positive pressure cabinet is used for supplying air outward after efficient filtering to complete a dust-free sterile environment needed by the cell exposure experiment on the other hand. A novel device is provided for safe accomplishment of the scientific environment of the cell exposure in an aerosol sample.
Description
Technical field:
The utility model provides a kind of positive negative pressure Campatible cellular exposure aseptic shielding cabinet device.
Background technology:
The aerosol of cell expose exposure experiment be in recent years bio-science, medical and health, animals and plants learn and toxicologic study in emerging experimental technique; It not the use-testing animal, only carry out the scientific experiment that the cell aerosol exposes with cell or local organization alternate method; The scientific experiment that this cell aerosol exposes can affact the not only convenient succinctly but also scientific and precise of cell interior mechanism, deep experimentation, experimental data is accurately careful; Can also shorten that the test period saves time and resource, extensive and far-reaching practicality and researching value arranged international and domestic; Exposing at the aerosol of cell needs one can guarantee that cleaning that cell survives normally, grow and test, dustless, gnotobasis aerosol exposure meanwhile are again need some poisonous and hazardous materials are aerosolized in the exposure experiment; Be easy to diffusion and operating environment of pollution, even the operator is damaged; This is the contradiction that not only need link together but also can not hold mutually.Also not have in the industry at present satisfactorily to accomplish the required professional device of this type of test specially, can't make things convenient for, safety, the aerosol that carries out cell accurately, reliably exposes exposure experiment, thus this type of scientific research that has restricted deeply and develop; Therefore designing and developing the aseptic shielding cabinet device of a kind of positive negative pressure Campatible cellular exposure just becomes current urgent needs.
The utility model content:
For addressing the above problem; The purpose of the utility model provides the aseptic shielding cabinet device of a kind of positive negative pressure Campatible cellular exposure; But expose disinfection in the exposure experiment and, survive normally, grow and test to guarantee cell through the positive pressure operation cabinet of the cleaning of high efficiency filter forced draft, dustless, gnotobasis at the aerosol of cell.Same the other side is an antipollution aerosol generation negative pressure shielding cabinet that has suction function; The aerosol of accomplishing hazardous and noxious substances takes place, transmission and contamination exposure test, and the waste gas adsorption treatment device that whole shielding cabinet bottom has the negative pressure-pumping effect can expose safety dumping after some poisonous and hazardous material adsorption treatment that produce with aerosol.The aseptic shielding cabinet ability of this positive negative pressure Campatible cellular exposure safety, accurate, convenient, the reliable aerosol of satisfactorily accomplishing cell required in bio-science, medical and health, animals and plants and the toxicologic study expose exposure experiment, can be applied in widely in the scientific research in a plurality of fields such as toxicity, biology, medical treatment, environmental protection, military affairs, chemical industry.
For realizing above-mentioned purpose; The technical scheme of the utility model is the aseptic shielding cabinet device of a kind of positive negative pressure Campatible cellular exposure; Said device comprises that the positive pressure operation cabinet of band high efficiency filter forced draft system dust-free sterile and the other side are the associating two-phase cabinets that has the anti-pollution aerosol generation negative pressure shielding cabinet composition of suction function; Wherein the stifling and ozonateur of ultraviolet germicidal, gas is housed with the dust-free sterile positive pressure operation cabinet inside of high efficiency filter forced draft system; The back placement cellular exposure contamination frame that can be used to carry out disinfection carries out the aseptic viral infection test of cellular exposure; The blast inlet that is installed in the top in the whole experiment is outwards carried the dust-free sterile fresh air from top to bottom through what high efficiency filter forced draft system filtration did not stop in the positive pressure operation cabinet, in this operation cabinet, form positive pressure environment and can prevent effectively that ambient atmos from invading and cause bacterial contamination; And the other side is the anti-pollution aerosol generation negative pressure shielding cabinet that has suction function; Inside is placed with contamination material aerosol dispenser and transmission valve and pipeline and is used for contaminating that the aerosol of material takes place, quantitatively transmission and waste gas are discharged at the inner rear of negative pressure shielding cabinet and had the negative pressure suction opening to be connected to the waste gas adsorption treatment device that whole shielding cabinet bottom has the negative pressure-pumping effect through the negative pressure pipe connecting; Air makes negative pressure shielding cabinet inside keep the negative pressure state with respect to the external world at any time in the suction cabinet that in whole experiment, does not stop, and toxic and harmfuls such as the intravital contamination aerosol of negative pressure shielding cabinet can not leak to pollute operator and extraneous space but directly be inhaled into and carry out safety row after the harmless treatment in the waste gas adsorption treatment device; Positive pressure operation cabinet and two cabinets of negative pressure shielding cabinet closely link to each other, and are middle through pipeline and valve connection, but the active clapboard of folding is equipped with in the central dividing plate bottom, and two cabinet front upper all have the tensimeter that shows the inside and outside pressure reduction of cabinet separately; All have separately in the middle of two cabinet fronts and can slide up and down the glass port of opening or closing and be used for effectively air-isolation and the handled easily personnel operation that makes an experiment.
The positive pressure operation cabinet of described band high efficiency filter forced draft system dust-free sterile and the other side are to have the associating two-phase cabinet that the anti-pollution aerosol generation negative pressure shielding cabinet of suction function is formed; Be that framework is made with metal sheet and section bar in its outside of experimental implementation cabinet made from metallic substance, the inner liner that adopts stainless steel plate to make can prevent effectively to pollute and help sterilizing and cleaning; Cellular exposure contamination frame is equipped with in positive pressure operation cabinet inside has the petridish that holds the cell lamina membranacea to be used for carrying out the cellular exposure exposure experiment with incubation pond and exposure chamber; The forced draft system that the band high efficiency filter is installed by the blast inlet at positive pressure operation cabinet top through gasket in the whole experiment with the outside air filtration sterilization after continuously input forms positive pressure environment, ultraviolet germicidal, the gas that the band automatic timing switch is installed in positive pressure operation cabinet inside is fumigated and ozonateur can be to the sterilization that carries out disinfection of cabinet internal timing; The anti-pollution aerosol generation negative pressure shielding cabinet that has suction function; Inside is placed with contamination material aerosol dispenser and comprises the contamination material and quantitatively import unit, contamination material aerosol vaporizer and aerosol and collect transmission interface, passes central dividing plate through transmission valve and pipeline and is connected to the exposure chamber pair cell that is equipped with on the cellular exposure contamination frame positive pressure operation cabinet inside and carries out exposure experiment.
The beneficial effect of the utility model is: provide a kind of positive negative pressure Campatible cellular exposure aseptic shielding cabinet device, exposed in the exposure experiment at the aerosol of cell and not only can guarantee that cell under cleaning, dustless, the gnotobasis is survived normally, grow simultaneously but also the cell contamination that can accomplish hazardous and noxious substances safely exposes and tests; Solved this compatible difficult problem that not only need link together but also can not pollute mutually; The aerosol that can satisfactorily accomplish cell required in the toxicologic study safely, accurately, conveniently, reliably exposes exposure experiment, in the scientific experiment under the aerosol sample new device is provided for accomplishing cellular exposure in the wide spectrums such as toxicity, biology, medical treatment, environmental protection, military affairs, chemical industry safely.
Description of drawings:
Accompanying drawing 1 is looked synoptic diagram for the master of the utility model structure
Accompanying drawing 2 is the schematic top plan view of the utility model structure
Accompanying drawing 3 is the schematic side view of the utility model structure
Wherein among the figure:
1, negative pressure shielding cabinet
2, positive pressure operation cabinet
3, high efficiency filter forced draft system
4, ultraviolet germicidal
5, the stifling and ozonateur of gas
6, cellular exposure contamination frame
7, expose the chamber
8, petridish
9, warm bathing pool
10, waste gas adsorption treatment device
11, negative pressure suction opening
12, aerosol dispenser
13, quantitatively import unit
14, vaporizer
15, collect transmission interface
16, glass port
17, tensimeter
18, central dividing plate
19, blast inlet
20, active clapboard
21, negative pressure pipe connecting
Embodiment:
In conjunction with accompanying drawing 1, accompanying drawing 2 and accompanying drawing 3 and embodiment the utility model is explained.
Said device comprises that positive pressure operation cabinet 2 bodies of band high efficiency filter forced draft system 3 dust-free sterile and the other side are the associating two-phase cabinets that has the anti-pollution aerosol generation negative pressure shielding cabinet 1 body composition of suction function; Wherein the stifling and ozonateur 5 of ultraviolet germicidal 4, gas is housed with the dust-free sterile positive pressure operation cabinet 2 body inside of high efficiency filter forced draft system 3; The back placement cellular exposure contamination frame 6 that can be used to carry out disinfection carries out the aseptic viral infection test of cellular exposure; The powered air-purifying effect of the blast inlet 19 that is installed in the top in the whole experiment through band high efficiency filter forced draft system 3 do not stop outwards carries the dust-free sterile fresh air from top to bottom in positive pressure operation cabinet 2 bodies, in this operation cabinet, form positive pressure environment and can prevent effectively that ambient atmos from invading and cause bacterial contamination; And the other side is anti-pollution aerosol generation negative pressure shielding cabinet 1 body that has suction function; Inside is placed with contamination material aerosol dispenser 12 and transmission valve and pipeline and is used for contaminating that the aerosol of material takes place, quantitatively transmission and waste gas discharge work; There is negative pressure suction opening 11 to be connected to the waste gas adsorption treatment device 10 that whole shielding cabinet bottom has the negative pressure-pumping effect at the inner rear of negative pressure shielding cabinet 1 body through negative pressure pipe connecting 21; Air makes negative pressure shielding cabinet 1 body inside keep with respect to the negative pressure state in the external world at any time and the air flowing of ecto-entad is arranged in the suction cabinet that in whole experiment, does not stop, and toxic and harmfuls such as negative pressure shielding cabinet 1 intravital contamination aerosol carry out safety dumping after the harmless treatment with regard to not leaking to pollute operator and extraneous space but directly be inhaled in the waste gas adsorption treatment device 10; Two cabinets of positive pressure operation cabinet 2 bodies and negative pressure shielding cabinet 1 body closely link to each other; Middle through pipeline and valve connection; But 20, two cabinet front upper of active clapboard of folding are equipped with in central dividing plate 18 bottoms the tensimeter 17 that shows the inside and outside pressure reduction of cabinet is arranged all separately; All have separately in the middle of two cabinet fronts and can slide up and down the glass port 16 of opening or closing and be used for effectively air-isolation and the handled easily personnel operation that makes an experiment.
Positive pressure operation cabinet 2 bodies of described band high efficiency filter forced draft system 3 dust-free sterile and the other side are to have the associating two-phase cabinet that anti-pollution aerosol generation negative pressure shielding cabinet 1 body of suction function is formed; Be that framework is made with metal sheet and section bar in its outside of experimental implementation cabinet made from metallic substance, the inner liner that adopts stainless steel plate to make can prevent effectively to pollute and help sterilizing and cleaning; Cellular exposure contamination frame 6 is equipped with in positive pressure operation cabinet 1 body inside has the petridish 8 that holds the cell lamina membranacea to be used for carrying out the cellular exposure exposure experiment with incubation pond 9 and exposure chamber 7; Input continuously after positive pressure operation cabinet 2 body tops have forced draft system 3 that blast inlet 19 is equipped with the band high efficiency filter through gasket with the outside air filtration sterilization, the stifling and ozonateur 5 of ultraviolet germicidal 4, the gas of band automatic timing switch is installed in positive pressure operation cabinet 2 body inside can be to the sterilization that carries out disinfection of cabinet internal timing; Anti-pollution aerosol generation negative pressure shielding cabinet 1 body that has suction function; Inside is placed with contamination material aerosol dispenser 12 and comprises the contamination material and quantitatively import unit 13, contamination material aerosol vaporizer 14 and aerosol and collect transmission interface 15, passes central dividing plate 18 through transmission valve and pipeline and is connected to exposure chamber 7 pair cells that are equipped with on the cellular exposure contamination frame 6 positive pressure operation cabinet 2 body inside and carries out exposure experiment.
Embodiment:
Power-on at first when using the aseptic shielding cabinet device of a kind of positive negative pressure Campatible cellular exposure of the utility model; Downward sliding closing glass port 16 also fumigates with ultraviolet germicidal 4, gas successively and cleaning-sterilizing is carried out in 5 pairs of positive pressure operation cabinets of ozonateur, 2 body inside; Open the waste gas adsorption treatment device 10 that whole shielding cabinet bottom has the negative pressure-pumping effect and make the inner negative pressure-pumping function that produces of negative pressure shielding cabinet 1 body; Make negative pressure shielding cabinet 1 body inside keep with respect to the negative pressure state in the external world at any time and the air flowing of ecto-entad is arranged, toxic and harmfuls such as negative pressure shielding cabinet 1 intravital contamination aerosol just can not leak and pollute operator and extraneous space; With high efficiency filter forced draft system 3 open make that high efficiency filter forced draft system 3 do not stop in positive pressure operation cabinet 2 bodies, outwards carry the dust-free sterile fresh air from top to bottom, in this operation cabinet, form positive pressure environment and can prevent effectively that ambient atmos from invading and cause bacterial contamination; Slide-open positive pressure operation cabinet 2 body glass ports 16 make progress; Open cellular exposure contamination frame 6 and will test used cell lamina membranacea and put into petridish 8 and incubation pond 9 and expose in the chamber 7 back airtight good, the glass port 16 of downward sliding closing positive pressure operation cabinet 2 bodies is so that carry out the cellular exposure exposure experiment; The glass port 16 of slide-open negative pressure shielding cabinet 1 body upwards; Required material of contaminating exposure being put into the contamination material of contamination material aerosol dispenser 12 quantitatively imports in unit 13 containers; Configure quantitative input speed and open contamination material aerosol dispenser 12 switches, the glass port 16 of sliding closing negative pressure shielding cabinet 1 exposes exposure experiment downwards; The aerosol that produces during 14 work of contamination material aerosol vaporizer enters into aerosol and collects transmission interface 15 and be connected to the transmission valve and pipeline passes central dividing plate 18 and enters into exposure chamber 7 pair cells that are equipped with on the cellular exposure contamination frame 6 positive pressure operation cabinet 2 body inside and expose exposure experiment; Experiment knot speed back elder generation is slide-open negative pressure shielding cabinet 1 body glass port 16 upwards; Close contamination material aerosol dispenser 12 switches; Stop 14 work of aerosol vaporizer; The glass port 16 of slide-open positive pressure operation cabinet 2 bodies is upwards opened cellular exposure contamination frame 6 and will test used cell lamina membranacea from petridish 8 and incubation pond 9 and expose in the chamber 7 taking-ups and analyze and post-processed work; Close high efficiency filter forced draft system 3 and the waste gas adsorption treatment device 10 that has the negative pressure-pumping effect respectively, two cabinet cleaning insides are clean, the glass port 16 back powered-down switches of two cabinets of the downward sliding closing of difference.
Claims (6)
1. aseptic shielding cabinet device of positive negative pressure Campatible cellular exposure; It is characterized in that: said device comprises that band high efficiency filter forced draft system (3) positive pressure operation cabinet (2) body and the other side are the associating two-phase cabinets that has negative pressure shielding cabinet (1) the body composition of suction function; Wherein ultraviolet germicidal (4) is equipped with in positive pressure operation cabinet (2) body inside, gas is stifling and ozonateur (5); The inner cellular exposure contamination frame (6) of placing; High efficiency filter forced draft system (3) outwards carries the dust-free sterile fresh air from top to bottom in positive pressure operation cabinet (2) body, in this operation cabinet, form positive pressure environment; And negative pressure shielding cabinet (1) body of the other side, the inner contamination material aerosol dispenser (12) of placing, the waste gas adsorption treatment device (10) that has negative pressure suction opening (11) connection to have the negative pressure-pumping effect at inner rear produce negative pressure; Two cabinets of positive pressure operation cabinet (2) body and negative pressure shielding cabinet (1) body closely link to each other; Be communicated with through pipeline and valve; The junction is provided with central dividing plate (18); But the active clapboard (20) of folding is equipped with in the bottom, and two cabinet front upper all have the tensimeter (17) that shows the inside and outside pressure reduction of cabinet separately; All have separately in the middle of two cabinet fronts and can slide up and down the glass port (16) of opening or closing.
2. the aseptic shielding cabinet device of positive negative pressure Campatible cellular exposure according to claim 1; It is characterized in that: described band high efficiency filter forced draft system (3) positive pressure operation cabinet (2) body and the other side are the associating two-phase cabinets that has negative pressure shielding cabinet (1) the body composition of suction function; Be that its outside metal sheet and section bar of spraying used of experimental implementation cabinet made from metallic substance is made framework, the inner liner that adopts stainless steel plate to make can prevent effectively to pollute and help sterilizing and cleaning.
3. the aseptic shielding cabinet device of positive negative pressure Campatible cellular exposure according to claim 1; It is characterized in that: described band high efficiency filter forced draft system (3) positive pressure operation cabinet (2) body; Cellular exposure contamination frame (6) is equipped with in inside; Form by the petridish (8) that holds the cell lamina membranacea and incubation pond (9) being arranged and exposing chamber (7); Petridish (8) can hold the experimental cell lamina membranacea; Petridish (8) is surrounded by incubation pond (9), expose chamber (7) be installed in petridish (8) and experimental cell lamina membranacea directly over, expose chamber (7) and be connected on the intravital aerosol collection transmission interface of negative pressure shielding cabinet (1) (15) through pipeline and valve.
4. the aseptic shielding cabinet device of positive negative pressure Campatible cellular exposure according to claim 1; It is characterized in that: described band high efficiency filter forced draft system (3) positive pressure operation cabinet (2) body; The high efficiency filter forced draft system (3) that install through gasket on top is a rectangular seal metal closed case; Its inner high efficiency filter adopts macromolecule filter material and built-in blower fan to be mounted to one; Its inlet end has a blast inlet (19) to be connected to the opening part at positive pressure operation cabinet (2) body top, and high efficiency filter forced draft system (3) outlet side is connected air-supply downwards with gasket all around with positive pressure operation cabinet (2) body.
5. the aseptic shielding cabinet device of positive negative pressure Campatible cellular exposure according to claim 1; It is characterized in that: described negative pressure shielding cabinet (1) body that has suction function; The inner contamination material aerosol dispenser of placing (12) comprises that the contamination material is quantitatively imported unit (13), contamination material aerosol vaporizer (14) is collected transmission interface (15) with aerosol, passes central dividing plate (18) through transmission valve and pipeline and is connected to positive pressure operation cabinet (2) body inside and cellular exposure is housed contaminates in the exposure chamber (7) on the frame (6).
6. the aseptic shielding cabinet device of positive negative pressure Campatible cellular exposure according to claim 1; It is characterized in that: described negative pressure shielding cabinet (1) body that has suction function; Inner rear has negative pressure suction opening (11) to be connected on the waste gas adsorption treatment device (10) that whole shielding cabinet bottom has the negative pressure-pumping effect through negative pressure pipe connecting (21); This device is made up of negative pressure vacuum fan and the solid-state adsorber of gac; The solid-state adsorber input terminus of gac is sealably coupled on the negative pressure suction opening (11) at the inner rear of negative pressure shielding cabinet (1) body through negative pressure pipe connecting (21), and the active carbon adsorber output terminal is connected on the negative pressure vacuum fan.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120382278 CN202322846U (en) | 2011-10-10 | 2011-10-10 | Positive-negative pressure compatible cell exposure sterility shielding cabinet device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120382278 CN202322846U (en) | 2011-10-10 | 2011-10-10 | Positive-negative pressure compatible cell exposure sterility shielding cabinet device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202322846U true CN202322846U (en) | 2012-07-11 |
Family
ID=46434833
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120382278 Expired - Fee Related CN202322846U (en) | 2011-10-10 | 2011-10-10 | Positive-negative pressure compatible cell exposure sterility shielding cabinet device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202322846U (en) |
-
2011
- 2011-10-10 CN CN 201120382278 patent/CN202322846U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120711 Termination date: 20131010 |