CN110479388B - Sterile operation device for stem cell preparation - Google Patents
Sterile operation device for stem cell preparation Download PDFInfo
- Publication number
- CN110479388B CN110479388B CN201910771499.9A CN201910771499A CN110479388B CN 110479388 B CN110479388 B CN 110479388B CN 201910771499 A CN201910771499 A CN 201910771499A CN 110479388 B CN110479388 B CN 110479388B
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- Prior art keywords
- sterile
- stem cell
- cell preparation
- communicated
- air
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 230000001954 sterilising effect Effects 0.000 claims abstract description 29
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 25
- 230000005540 biological transmission Effects 0.000 claims abstract description 13
- 239000000126 substance Substances 0.000 claims abstract description 11
- 241000894006 Bacteria Species 0.000 claims abstract description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 30
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 claims description 10
- 238000002955 isolation Methods 0.000 claims description 10
- 238000012546 transfer Methods 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 3
- 230000004087 circulation Effects 0.000 claims description 2
- 208000015181 infectious disease Diseases 0.000 abstract description 2
- 238000005286 illumination Methods 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 238000004140 cleaning Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000001914 filtration Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 238000004378 air conditioning Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000003749 cleanliness Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/20—Gaseous substances, e.g. vapours
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/20—Gaseous substances, e.g. vapours
- A61L2/202—Ozone
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L1/00—Enclosures; Chambers
- B01L1/04—Dust-free rooms or enclosures
Landscapes
- Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a sterile operating device for stem cell preparation, comprising: the box body comprises at least three operation areas which are independent from each other and are connected through a sterile transmission device; the chemical sterilization device is communicated with the operation area; and the physical sterilization device is connected with the box body. According to the sterile operation device for stem cell preparation, through the cooperation of the chemical sterilization device and the physical sterilization device, dead angle-free sterilization can be effectively realized, the sterile transmission device is arranged, sterile transmission can be realized in a plurality of links of stem cell preparation, and experimental failure caused by bacteria infection in the transmission process is avoided.
Description
Technical Field
The invention relates to the field of biomedical equipment, in particular to a sterile operation device for stem cell preparation.
Background
The sterile operation table is necessary equipment in the field of biomedical research, and can provide a sterile clean space for experimental operation of scientific researchers. Generally, ultraviolet sterilization is needed to be carried out on a microorganism sterile operation table before the experiment starts, but because light rays are transmitted along a straight line, if dead angles which cannot be irradiated by an ultraviolet lamp exist in the sterile operation table, bacteria are easy to hide in the dead angles; the operation process of the biological medicine experiment needs a relatively closed space, so that bacteria infection is avoided; however, in the stem cell preparation process, there is a need for transferring test articles during the operation between different preparation steps, and the test fails due to the failure to ensure the aseptic condition during the transfer step.
Disclosure of Invention
The invention provides a sterile operation device for preparing stem cells, which is sterile and clean and can realize sterile transmission of experimental products.
The technical scheme for solving the technical problems is as follows:
the invention provides a sterile operating device for stem cell preparation, comprising:
the box body comprises at least three operation areas which are independent from each other and are connected through a sterile transmission device;
the chemical sterilization device is communicated with the operation area;
And the physical sterilization device is connected with the box body.
The invention has the beneficial effects that: through the cooperation of chemical sterilization device and physical sterilization device, can effectually realize no dead angle sterilization, aseptic transmission device's setting can realize aseptic transmission in a plurality of links of stem cell preparation again, avoids the experiment failure with the in-process contamination of transmission causes.
Further, the operation area is a cavity surrounded by an operation table, a containment board and a front baffle, wherein the containment board and the front baffle are connected above the operation table, a front window of a cabinet body is arranged on the front baffle, a transparent isolation board is arranged on the front window, and fungus isolation operation gloves are arranged on the transparent isolation board.
Further, at least one sliding door is arranged on the containment plate, a door handle is arranged on the sliding door, and the sliding door is in sealing connection with the containment plate.
Further, the chemical sterilization device comprises an ozone generating device or an acetic acid generating device and a circulating fan, wherein the ozone generating device or the acetic acid generating device is connected with the box body, the circulating fan is connected with the box body, and the ozone generating device or the acetic acid generating device is communicated with the operation area.
Further, a sterilizer connecting hole is formed in the operation area, and an outlet of the circulating fan is communicated with the operation area through the sterilizer connecting hole.
Further, a flow equalizing film is arranged between the circulating fan and the operation area.
Further, a filtering device is arranged at the air outlet of the circulating fan.
Further, the physical sterilization device comprises an ultraviolet lamp.
The device further comprises an air exhaust device and an air return port, wherein the air exhaust device is an air exhaust fan, and an air inlet of the air exhaust fan is communicated with the operation area; the return air inlet is arranged at the lower part of the operation area, and the air inlet and the return air inlet are both provided with filters.
Further, the sterile transfer device includes:
a sterile transfer channel provided with at least three openings for communicating with the operating area;
the sterile conveying belt is arranged in the sterile conveying channel and is connected with the motor;
the two sterile air openings are arranged at two ends of the sterile conveying channel, and one sterile air opening is communicated with the exhaust fan.
The invention has compact structure, and compared with the biological safety cabinet, the occupied area is reduced by 30%; all production procedures are completed in the sterile operation device, the environment similar to the environment that the transmission of a biological safety cabinet needs to be performed outside the cabinet body is reduced, all production procedures are ensured to be completed in the sterile operation device, the purification safety level is high, the cross contamination links are less, and the preparation and purification safety environment of the product is further ensured; meanwhile, all production links are completed in the closed sterile operation device, and the production cleaning field is completed in the sterile operation device without direct contact with the outside, so that the efficiency of cleaning field is greatly improved, the cost of cleaning field is further reduced, and the thoroughness and the integrity of cleaning field are ensured.
Drawings
FIG. 1 is a schematic rear view of the present invention;
FIG. 2 is a schematic side view of the present invention;
Fig. 3 is a schematic view of the structure of the aseptic transfer apparatus of the present invention.
Description of the drawings:
1. the air inlet type air-conditioning system comprises a box body, an air inlet, a pointer type differential pressure meter, a power switch, an illumination switch, a control panel, a circulating fan, a filter, an illumination lamp, a sliding door, a handle, a return air inlet, a flow equalizing membrane, a return air box, a sterile conveying device, an air exhausting fan, an air exhausting filter, an integrated conveying window, a sterilizer connecting hole and a transparent partition plate.
Detailed Description
The principles and features of the present invention are described below with reference to the drawings, the examples are illustrated for the purpose of illustrating the invention and are not to be construed as limiting the scope of the invention.
The invention provides a sterile operating device for stem cell preparation, comprising:
The box body 1 comprises at least three operation areas which are independent from each other and are connected through a sterile transmission device; the chemical sterilization device is communicated with the operation area; and the physical sterilization device is connected with the box body.
The operation area is a cavity surrounded by an operation desk, a containment board 10 and a front baffle 11, wherein the containment board 10 and the front baffle 11 are connected above the operation desk, a front window of a cabinet body is arranged on the front baffle 11, a transparent isolation board 20 is arranged on the front window, and bacteria isolation operation gloves are arranged on the transparent isolation board 20.
As a further aspect of the present invention, at least one sliding door is provided on the containment plate 10, a door handle is provided on the sliding door, and the sliding door is connected with the containment plate in a sealing manner.
As a further scheme of the invention, the chemical sterilization device comprises an ozone generating device or an acetic acid generating device and a circulating fan 7, wherein the ozone generating device or the acetic acid generating device is connected with the box body, the circulating fan 7 is connected with the box body 1, and the ozone generating device or the acetic acid generating device is communicated with the operation area.
As a further scheme of the invention, a sterilizer connecting hole 19 is arranged on the operation area, and the outlet of the circulating fan 7 is communicated with the operation area through the sterilizer connecting hole 19.
As a further scheme of the invention, a flow equalizing membrane 13 is arranged between the circulating fan and the operation area.
As a further scheme of the invention, a filtering device 8 is arranged at the air outlet of the circulating fan.
As a further aspect of the invention, the physical sterilization device comprises an ultraviolet lamp.
As a further scheme of the invention, the air conditioner further comprises an air exhausting device and an air return port 12, wherein the air exhausting device is an air exhausting fan 16, and an air inlet 2 of the air exhausting fan 16 is communicated with an operation area; the return air inlet 12 is arranged at the lower part of the operation area, and filters are arranged at the air inlet 12 and the return air inlet 2, and as shown in fig. 2, an exhaust filter device 17 at the position where an exhaust fan is arranged can be arranged.
The box 1 is further provided with a pointer type differential pressure meter 3, a power switch 5, an illumination switch 5 and a control panel 6, wherein the pointer type differential pressure meter 3, the power switch 5, the illumination switch 5 and the control panel 6 are arranged on the upper portion of the box and are connected with the side wall of the box. The pointer type differential pressure gauge is used for displaying the pressure in the operation area, and the power switch is connected with the power supply and used for controlling the power supply; the illumination switch 5 is connected with an illumination lamp 9 arranged in the operation area and used for controlling the on and off of the illumination lamp.
As a further aspect of the present invention, the aseptic transfer device 15 includes:
a sterile transfer channel 151, said sterile transfer channel 151 having at least three openings 152 for communicating with an operating area;
A sterile conveyor 153, the sterile conveyor 153 being disposed within the sterile conveyor channel 151, the sterile conveyor being connected to a motor;
and two sterile air ports are arranged at two ends of the sterile conveying channel 151, wherein one sterile air port is communicated with the air exhaust fan 16.
As a further aspect of the present invention, a sliding door that can be opened and closed may be provided at the opening 152.
As a further aspect of the present invention, the above-described filter device and filter may employ an HVAC filter.
The invention relates to a sterile operation device for stem cell preparation, which mainly comprises a stainless steel frame, a stainless steel operation table, a fan, an HVAC filter, a PLC control system, a sterile transmission system and a sterilization system. The method is mainly used for providing an A-level sterile laminar flow environment which meets legal requirements and can protect samples, environment and personnel when various cells are prepared. When the device is used, the equipment purifies the environment B-level clean air into A-level standard and then sends the A-level standard to the operation desk to provide a sterile environment, meanwhile, the system air quantity is designed to be negative pressure, and the operation desk is provided with a glass isolation device to prevent the air in an operation area from overflowing and pollute the environment and operators.
The invention is used for being placed in a class B clean room and providing a completely sterile laminar flow environment for producing stem cells.
The invention also comprises a PLC control system, wherein the circulating fan, the exhaust fan, the illuminating lamp and the sterilizing lamp are all controlled by the PLC control system, and can be automatically controlled on a control panel, and the control system is designed with an operation mode and a sterilizing mode.
The equipment operation table is arranged in a partitioning manner according to the equipment required by stem cell operation, fully considers the influence of each equipment on laminar flow and cleanliness, and is separated by acrylic glass.
Operation mode: when the equipment is in a normal starting operation mode, the ultraviolet lamp is turned off, the front glass panel of the operating platform is lifted by 20cm, and ambient fresh air sequentially passes through the primary filter, the fan and HVAV from the air inlet at the lower part of the equipment to form a layer streaming to the operating platform area. The back part of the operation table is additionally provided with a return air box body 14, the return air box body 14 is communicated with the operation area, and laminar air flowing through the operation table enters the air duct to the return air box body 14 through the return air guide hole to form air quantity circulation. The top of the equipment is provided with an exhaust fan and a high-efficiency filter, and the equipment is started during production to exhaust 30% of used air quantity.
Sterilization mode: when sterilization is performed, the air exhaust fan is closed, the air inlet is closed, the glass panel is closed before the operation panel, the ozone or acetic acid generator is opened, the circulating fan is opened to perform microcirculation in a low air quantity mode, sterilizing substances are sent to all corners of the operation panel and the air duct, and after sterilization is finished, the air exhaust fan and the air inlet are opened to perform ventilation, and the sterilizing substances are discharged.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.
Claims (6)
1. A sterile handling device for stem cell preparation, comprising:
the box body comprises at least three operation areas which are independent from each other and are connected through a sterile transmission device;
the chemical sterilization device is communicated with the operation area;
The physical sterilization device is connected with the box body;
The sterile delivery device includes:
a sterile transfer channel provided with at least three openings for communicating with the operating area;
the sterile conveying belt is arranged in the sterile conveying channel and is connected with the motor;
the two sterile air openings are arranged at two ends of the sterile conveying channel, and one sterile air opening is communicated with the exhaust fan;
The chemical sterilization device comprises an ozone generation device or an acetic acid generation device and a circulating fan, wherein the ozone generation device or the acetic acid generation device is connected with the box body, the circulating fan is connected with the box body, and the ozone generation device or the acetic acid generation device is communicated with the operation area;
The operating area is provided with a sterilizer connecting hole, and an outlet of the circulating fan is communicated with the operating area through the sterilizer connecting hole;
And a flow equalizing film is arranged between the circulating fan and the operation area.
2. The sterile handling device for stem cell preparation according to claim 1, wherein the handling area is a cavity defined by a handling table and a containment plate and a front baffle plate connected above the handling table, a cabinet front window is provided on the front baffle plate, a transparent isolation plate is provided on the front window, and a bacteria isolation handling glove is provided on the transparent isolation plate.
3. The sterile handling device for stem cell preparation according to claim 2, wherein at least one sliding door is provided on the containment sheet, a door handle is provided on the sliding door, and the sliding door is in sealing connection with the containment sheet.
4. The aseptic manipulation device for stem cell preparation according to claim 1, wherein a filter device is provided at an air outlet of the circulation fan.
5. The sterile handling device for stem cell preparation of claim 1, wherein the physical sterilization device comprises an ultraviolet light lamp.
6. The aseptic manipulation device for stem cell preparation according to claim 1, further comprising an exhaust device and an air return, wherein the exhaust device is an exhaust fan, and an air inlet of the exhaust fan is communicated with the manipulation area; the return air inlet is arranged at the lower part of the operation area, and the air inlet and the return air inlet are both provided with filters.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910771499.9A CN110479388B (en) | 2019-08-21 | 2019-08-21 | Sterile operation device for stem cell preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910771499.9A CN110479388B (en) | 2019-08-21 | 2019-08-21 | Sterile operation device for stem cell preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110479388A CN110479388A (en) | 2019-11-22 |
| CN110479388B true CN110479388B (en) | 2024-05-17 |
Family
ID=68552393
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910771499.9A Active CN110479388B (en) | 2019-08-21 | 2019-08-21 | Sterile operation device for stem cell preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110479388B (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111485739A (en) * | 2020-04-15 | 2020-08-04 | 江苏超净电子系统工程有限公司 | Cleaning level crossing protection device installed in modularized mode |
| IT202000009868A1 (en) * | 2020-05-05 | 2021-11-05 | Gilardoni Spa | SANITATION METHOD AND APPARATUS FOR THE KILLING OF THE VIRAL AND BACTERIAL LOAD PRESENT ON OBJECTS. |
| CN113181979B (en) * | 2021-04-26 | 2023-05-26 | 苏州苏因智启生物科技有限公司 | Biological sample processing environment retention system |
| CN113462563B (en) * | 2021-06-24 | 2022-12-27 | 冰山松洋生物科技(大连)有限公司 | Sterile transfer system based on cell workstation and collection room UNICOM |
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| WO2019012696A1 (en) * | 2017-07-14 | 2019-01-17 | 株式会社日立産機システム | Safety cabinet and method for sterilizing same |
| CN210752732U (en) * | 2019-08-21 | 2020-06-16 | 和泓（厦门）生物技术有限公司 | A aseptic manipulation device for stem cell preparation |
-
2019
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Patent Citations (10)
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| EP2133101A1 (en) * | 2007-03-28 | 2009-12-16 | Sanyo Electric Co., Ltd. | System for maintaining sterile environment |
| CN102500434A (en) * | 2011-11-08 | 2012-06-20 | 上海东富龙科技股份有限公司 | Isolator for sterility detection process |
| CN202315914U (en) * | 2011-11-14 | 2012-07-11 | 四川汇宇制药有限公司 | Inspection isolator |
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| Publication number | Publication date |
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| CN110479388A (en) | 2019-11-22 |
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