CN113462563A - Sterile transfer system based on cell workstation and collection room UNICOM - Google Patents
Sterile transfer system based on cell workstation and collection room UNICOM Download PDFInfo
- Publication number
- CN113462563A CN113462563A CN202110702710.9A CN202110702710A CN113462563A CN 113462563 A CN113462563 A CN 113462563A CN 202110702710 A CN202110702710 A CN 202110702710A CN 113462563 A CN113462563 A CN 113462563A
- Authority
- CN
- China
- Prior art keywords
- sample
- speed
- transfer
- conveyor belt
- controller
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65G—TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
- B65G43/00—Control devices, e.g. for safety, warning or fault-correcting
- B65G43/08—Control devices operated by article or material being fed, conveyed or discharged
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to a sterile transfer system based on communication of a cell workstation and a collection room, which comprises: the transfer bin main body is used for transferring samples; a scanner for scanning the sample height to obtain a sample height H; a conveyor belt for transferring the sample by rotating the conveyor belt; the image collector is used for collecting a sample image; the track is used for completing the real-time acquisition of the sample image by the image acquisition device through the track; a mass sensor disposed below the conveyor belt and abutting the transfer bin gate for detecting a sample mass M; the motor is used for providing power for the conveyor belt; the adjusting valve is used for adjusting the speed of the conveyor belt; the conveying bin door is used for isolating the inside of the conveying bin from the outside of the conveying bin; the sealing strip is tightly connected with the transfer bin door when the transfer bin door is closed so as to seal the interior of the transfer bin; thereby can reduce transit time, avoid the sample contaminated, quick convenient, improve work efficiency, moreover, can also reduce the fluctuation of sample in the transmission course, improve the stability of sample.
Description
Technical Field
The invention relates to the technical field of cell culture devices, in particular to an aseptic transfer system based on communication between a cell workstation and a collection room.
Background
The sample needs to be collected in the collection chamber, the incubation process is performed in the cell workstation, and the sample needs to be transferred from the collection chamber to the cell workstation for incubation. The cleanliness requirement of the collection chamber is high, the cleanliness requirement of a room where the cell workstation is stored is also high, but the cleanliness of the sample transfer process cannot be guaranteed.
The sample transfer device is an auxiliary device for transferring a transfer box or a transfer tank from the outdoor to the indoor, and is mainly used for transferring samples between a clean area and a clean area, between the clean area and a non-clean area or between areas with different temperature difference environments, so that the door opening times are reduced, and the indoor pollution is reduced to the minimum degree. Present sample transfer device is equipped with the through-hole in the wall body mainly, and transfer device establishes wherein, and both sides all have the mechanism of opening a door, and outdoor people's accessible is opened the door and is put into the device with the sample, then the indoor door of indoor personnel's accessible is opened and is got the sample, avoids the environmental pollution to laboratory inside, but does not realize automatic conveying sample, also can not guarantee that the sample can not pollute at the in-process of transmission, also can not guarantee the stability of sample.
Disclosure of Invention
Therefore, the invention provides an aseptic transfer system based on communication between a cell workstation and a collection chamber, which can effectively solve the technical problem that the stability of a sample cannot be improved by adjusting the sample transfer speed through the sample shaking degree and the gradient of a conveyor belt in the prior art.
In order to achieve the above object, the present invention provides an aseptic transfer system based on communication between a cell workstation and a collection room, comprising:
the transfer bin main body is used for transferring samples;
the scanner is connected with the transfer bin main body and used for scanning the height of the sample to obtain the height H of the sample;
the conveying belt is connected with the conveying bin main body, is arranged in the conveying bin main body and is used for conveying the sample by rotating the conveying belt;
the image collector is connected with the transfer bin main body and is used for collecting a sample image;
the track is connected with the transfer bin main body, is arranged in the transfer bin main body, is provided with an image collector and is used for completing the real-time collection of a sample image by the image collector through the track;
the mass sensor is connected with the conveyor belt, arranged below the conveyor belt and abutted against the conveying bin gate and used for detecting the mass M of the sample;
the motor is connected with the conveyor belt and used for providing power for the conveyor belt;
the adjusting valve is connected with the motor and used for adjusting the speed of the conveyor belt;
the transfer bin door is connected with the transfer bin main body, is used for isolating the interior of the transfer bin from the exterior of the transfer bin, is provided with a door switch, is provided with a torsion, and realizes the opening/closing of the transfer bin door through the torsion;
the sealing strip is connected with the main body of the transfer bin and arranged at the opening of the transfer bin, and the door of the transfer bin is tightly connected with the sealing strip when closed so as to seal the interior of the transfer bin;
the controller is connected with the scanner, the conveyor belt, the image collector, the track, the mass sensor, the motor and the regulating valve and is used for controlling the transmission process of the sample;
when the sterile transfer system transfers a sample, the controller determines a sample transfer speed parameter A according to the sample mass M and the sample height H, when the determination is finished, the sample transfer speed parameter A is compared with a preset sample transfer speed parameter to determine the initial sample transfer speed, the sample shaking degree D is compared with the preset sample shaking degree to determine the speed regulating quantity of the conveyor belt, the conveyor belt gradient P is compared with the preset conveyor belt gradient to determine a speed correction coefficient and determine a speed correction quantity E through a preset formula, and when the determination is finished, the speed of the conveyor belt is controlled and regulated through the regulating valve;
the sample shaking degree D is obtained by the sample initial image F0 and the sample actual image F.
Further, when the sterile transfer system transfers a sample, the controller acquires a detection result of the mass sensor and sets the detection result as the sample mass, meanwhile, the controller analyzes a scanning result of the scanner to obtain the height of the sample and sets the height of the sample as the sample height H, when the setting is finished, the controller determines a sample transfer speed parameter A according to the sample mass M and the sample height H,
A＝(M/M0)+(H/H0)；
in the formula, M0 represents a sample annotation mass, and H0 represents a sample standard height.
Further, when the sample transfer speed parameter a is determined to be complete, the controller compares the sample transfer speed parameter a with a preset sample transfer speed parameter to determine a sample transfer initial speed, and when the controller determines that the initial speed of the conveyor belt for transferring the sample is Vi, the controller starts the motor, controls the adjusting valve to adjust the conveyor belt speed to Vi, and sets i to 1, 2,3, and 4;
the controller is provided with preset sample transfer speed parameters and a conveyor belt initial speed, wherein the preset sample transfer speed parameters comprise a first preset sample transfer speed parameter A1, a second preset sample transfer speed parameter A2 and a third preset sample transfer speed parameter A3, wherein A1 is more than A2 and is more than A3; the conveyor initial speeds comprise a conveyor first initial speed V1, a conveyor second initial speed V2, a conveyor third initial speed V3 and a conveyor fourth initial speed V4;
if A < A1, the controller determines that the initial speed of the conveyor belt for transferring the sample is V1;
if A1 is not less than A and is less than A2, the controller judges that the initial speed of the conveyor belt for transferring the samples is V2;
if A2 is not less than A and is less than A3, the controller judges that the initial speed of the conveyor belt for transferring the samples is V3;
if A is larger than or equal to A3, the controller judges that the initial speed of the conveyor belt for conveying the sample is V4.
Further, when the conveyor belt transfers the sample at the initial speed Vi, setting i to be 1, 2,3,4, and the controller acquires the shaking degree of the sample in the transfer process and sets the shaking degree to be a sample shaking degree D, when the setting is completed, the controller compares the sample shaking degree D with the preset sample shaking degree to determine a speed adjustment amount of the conveyor belt, and when the controller determines that the speed adjustment amount of the conveyor belt is Qi, the controller controls the adjusting valve to adjust the speed of the conveyor belt, and the adjustment amount is Qi, and sets i to be 1, 2,3, 4;
the controller is further provided with preset sample shaking degrees and a conveyor belt speed regulating amount, wherein the preset sample shaking degrees comprise a first preset sample shaking degree D1, a second preset sample shaking degree D2 and a third preset sample shaking degree D3, and D1 is more than D2 and less than D3; the conveyor speed regulating quantity comprises a first conveyor speed regulating quantity Q1, a second conveyor speed regulating quantity Q2, a third conveyor speed regulating quantity Q3 and a fourth conveyor speed regulating quantity Q4, wherein Q1 is more than Q2 and more than Q3 and more than Q4;
if D < D1, the controller determines that the amount of speed adjustment of the conveyor belt is Q1;
if D1 is not less than D < D2, the controller judges that the speed regulating quantity of the conveyor belt is Q2;
if D2 is not less than D < D3, the controller judges that the speed regulating quantity of the conveyor belt is Q3;
if D is larger than or equal to D3, the controller judges that the speed adjustment amount of the conveyor belt is Q4.
Further, when the controller determines that the speed regulating quantity of the conveyor belt is Qi, the controller sets the gradient of the front part of the conveyor belt, which is to be accessed by the sample, as the gradient P of the conveyor belt, and when the setting is finished, the controller compares the gradient P of the conveyor belt with the preset gradient of the conveyor belt to determine a speed correction coefficient;
the controller is further provided with a preset conveyor belt gradient and a speed correction coefficient, wherein the first preset conveyor belt gradient P1, the second preset conveyor belt gradient P2 and the third preset conveyor belt gradient P3 are included, and P1 is larger than P2 and P3; the speed correction coefficient includes a speed first correction coefficient σ 1, a speed second correction coefficient σ 2, a speed third correction coefficient σ 3, and a speed fourth correction coefficient σ 4, where σ 1 < σ 2 < σ 3 < σ 4 and σ 1+ σ 2+ σ 3+ σ 4 are 2;
if P < P1, the controller determines that the correction coefficient of the speed adjustment amount is σ 1;
if P1 is less than or equal to P < P2, the controller determines that the correction coefficient of the speed adjustment amount is sigma 2;
if P2 is less than or equal to P < P3, the controller determines that the correction coefficient of the speed adjustment amount is sigma 3;
if P is greater than or equal to P3, the controller determines that the correction factor for the speed adjustment amount is σ 4.
Further, when the controller determines that the speed correction coefficient is σ i, i is set to 1, 2,3,4, the controller determines a speed correction amount E,
when i is 1, E is set to Q1 × σ 1;
when i is 2, E is set to Q2 × σ 2;
when i is 3, E is set to Q3 × σ 3;
when i is 4, E is set to Q4 × σ 4.
Further, when the controller determines the speed correction amount E, the controller controls the adjustment valve to correct the speed of the conveyor belt, the corrected speed adjustment amount is Q ', Q' ═ Qi ± E is set, when the gradient in front of the sample is an ascending slope, Q '═ Qi + E, and when the gradient in front of the sample is a descending slope, Q' ═ Qi-E, and after the controller corrects the speed adjustment amount, the controller controls the adjustment valve to adjust the speed of the conveyor belt, and i is set to 1, 2,3, 4.
Further, before the sterile transfer system transfers the sample, the controller acquires an image of the sample collected by the image collector as a sample initial image F0, during the transfer process of the sample, the controller acquires the image of the sample collected by the image collector in real time as a sample actual image F, and when the acquisition is completed, the controller compares the sample initial image F0 with the sample actual image F to obtain a sample shaking degree D.
Compared with the prior art, the invention has the advantages that the invention arranges the transmission bin main body, the transmission bin door, the transmission belt, the image collector, the sealing strip and the controller, one end of the transmission bin main body is arranged in the collector, the other end is arranged in the culture chamber, the sample is placed in the transmission bin main body, the transmission bin door is closed, the transmission bin main body is isolated from the outside through the sealing strip, the transmission of the sample from the collector to the culture chamber is realized through the transmission belt, when the sample is transmitted, the controller determines the sample transmission speed parameter according to the sample quality and the sample height, when the determination is finished, the sample transmission speed parameter is compared with the preset sample transmission speed parameter to determine the sample transmission initial speed, the sample shaking degree is compared with the preset sample shaking degree to determine the speed regulating quantity of the transmission belt, the gradient of the transmission belt is compared with the gradient of the preset transmission belt to determine the speed correcting coefficient, and the speed correcting quantity is determined through the preset formula And when the determination is finished, controlling and adjusting the speed of the conveying belt through the adjusting valve. Thereby can confirm sample transmission initial velocity through sample quality and sample height to rock degree and conveyer belt slope through the sample and adjust sample transmission velocity, and then can reduce transit time, avoid the sample contaminated, quick convenient, improve work efficiency, moreover, can also reduce the fluctuation of sample in the transmission course, improve the stability of sample.
Particularly, the sealing strip is arranged, so that the sealing strip is tightly attached to the sealing strip when the door of the transfer bin is closed, the door switch is used for pressing the sealing strip to ensure the air tightness of the main body of the transfer bin, and the sample can be effectively prevented from being polluted.
Particularly, the transfer bin main body is directly connected with the cell workstation, so that the transfer bin is convenient for sample transmission and has a cleaning function.
Further, the invention determines a sample transfer speed parameter A according to the sample mass M and the sample height H, when the determination is completed, the sample transfer speed parameter A is compared with a preset sample transfer speed parameter to determine a sample transfer initial speed, the sample shaking degree D is compared with a preset sample shaking degree to determine a speed adjustment amount of the conveyor belt, the conveyor belt gradient P is compared with a preset conveyor belt gradient to determine a speed correction coefficient and determine a speed correction amount E through a preset formula, and when the determination is completed, the speed of the conveyor belt is controlled and adjusted through an adjusting valve, wherein the sample shaking degree D is obtained through a sample initial image F0 and a sample actual image F. Thereby can confirm sample transmission initial velocity through sample quality and sample height to rock degree and conveyer belt slope through the sample and adjust sample transmission velocity, and then can reduce transit time, avoid the sample contaminated, quick convenient, improve work efficiency, moreover, can also reduce the fluctuation of sample in the transmission course, improve the stability of sample.
Furthermore, the sample mass M, the sample height H and a preset formula determine the sample transfer speed parameter A, so that the sample transfer initial speed can be determined through the sample mass and the sample height, and the sample transfer speed is adjusted through the sample shaking degree and the conveyor belt gradient, so that the transfer time can be reduced, the sample is prevented from being polluted, the operation is rapid and convenient, the working efficiency is improved, the fluctuation of the sample in the transfer process can be reduced, and the stability of the sample is improved.
Furthermore, the sample transfer initial speed is determined by comparing the sample transfer speed parameter A with a preset sample transfer speed parameter, so that the sample transfer initial speed can be determined according to the sample quality and the sample height, and the sample transfer speed is adjusted according to the sample shaking degree and the conveyor belt gradient, so that the transfer time can be reduced, the sample is prevented from being polluted, the operation efficiency is improved, the fluctuation of the sample in the transfer process can be reduced, and the stability of the sample is improved.
Furthermore, the sample shaking degree D is compared with the preset sample shaking degree to determine the speed regulating quantity of the conveyor belt, so that the initial sample transmission speed can be determined according to the sample quality and the sample height, and the sample transmission speed is regulated according to the sample shaking degree and the conveyor belt gradient, so that the transfer time can be reduced, the sample is prevented from being polluted, the operation is fast and convenient, the working efficiency is improved, the fluctuation of the sample in the transmission process can be reduced, and the stability of the sample is improved.
Furthermore, the speed correction coefficient is determined by comparing the gradient P of the conveying belt with the preset gradient of the conveying belt, and the speed correction quantity E is determined by the preset formula, wherein the speed correction coefficient aims to improve the accuracy of calculation, so that the initial speed of sample transmission can be determined by the sample quality and the sample height, and the sample transmission speed is adjusted by the sample shaking degree and the gradient of the conveying belt, so that the transfer time can be reduced, the sample is prevented from being polluted, the speed and the convenience are realized, the working efficiency is improved, the fluctuation of the sample in the transmission process can be reduced, and the stability of the sample is improved.
Drawings
FIG. 1 is a schematic view of the interior of a transfer chamber body of a sterile transfer system based on communication between a cell workstation and a collection chamber according to an embodiment of the present invention;
FIG. 2 is an overall schematic diagram of a transfer chamber body of the sterile transfer system based on the communication between a cell workstation and a collection chamber according to the embodiment of the invention;
FIG. 3 is a schematic structural diagram of a sterile transfer system based on communication between a cell workstation and a collection room according to an embodiment of the present invention;
the notation in the figure is: 1. a collection chamber; 2. a culture chamber; 3. a cell workstation; 41. a transfer bin main body; 42. a scanner; 43. a conveyor belt; 44. a track; 441. an image collector; 45. a mass sensor; 46. a transfer bin gate; 461. a door switch; 462. twisting; 47. a sealing strip; 48. and a door hinge.
Detailed Description
In order that the objects and advantages of the invention will be more clearly understood, the invention is further described below with reference to examples; it should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only for explaining the technical principle of the present invention, and do not limit the scope of the present invention.
It should be noted that in the description of the present invention, the terms of direction or positional relationship indicated by the terms "upper", "lower", "left", "right", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, which are only for convenience of description, and do not indicate or imply that the device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
Furthermore, it should be noted that, in the description of the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Referring to fig. 1, fig. 2 and fig. 3, fig. 1 is a schematic view of an interior of a sterile transfer system transfer chamber body based on a cell workstation communicating with a collection chamber according to an embodiment of the present invention, fig. 2 is a schematic view of an entire sterile transfer system transfer chamber body based on a cell workstation communicating with a collection chamber according to an embodiment of the present invention, fig. 3 is a schematic view of a structure of a sterile transfer system based on a cell workstation communicating with a collection chamber according to an embodiment of the present invention, and the present invention provides a sterile transfer system based on a cell workstation communicating with a collection chamber, including:
a transfer chamber body 41 for transferring a sample;
a scanner 42 connected to the transfer chamber body 41 for performing a sample height scan to obtain a sample height H;
a conveyor belt 43 connected to the transfer chamber body 41, disposed in the transfer chamber body 41, and adapted to transfer the sample by rotating the conveyor belt;
an image collector 441 connected to the transfer chamber body 41 for collecting a sample image;
the rail 44 is connected with the transfer bin main body 41, is arranged in the transfer bin main body 41, is provided with an image collector 441, and is used for completing the real-time collection of a sample image by the image collector 441 through the rail 44;
a mass sensor 45 connected to the conveyor belt 43, disposed below the conveyor belt 43 and abutting against the transfer bin gate 46, for detecting the sample mass M;
a motor (not shown) connected to the conveyor belt 43 for powering the conveyor belt 43;
a regulating valve (not shown in the figure) connected with the motor and used for regulating the speed of the conveyor belt;
a transfer chamber door 46 connected to the transfer chamber main body 41 for isolating the inside of the transfer chamber from the outside of the transfer chamber, wherein a door switch 461 is provided thereon, a torsion 462 is provided on the door switch 461, and the opening/closing of the transfer chamber door 46 is realized by the torsion 462;
the sealing strip 47 is connected with the transfer bin main body 41 and arranged at the position of the transfer bin opening, and the transfer bin door 46 is tightly connected with the sealing strip 47 when being closed so as to seal the interior of the transfer bin;
a controller (not shown) connected to the scanner 42, the conveyor belt 43, the image collector 441, the rail 44, the mass sensor 45, the motor, and the regulating valve, for controlling the sample transferring process;
specifically, the sterile transfer system further includes door hinges 48 respectively coupled to the transfer chamber door 46 and the transfer chamber body 41 for connecting the transfer chamber door 46 and the transfer chamber body 41.
Specifically, the sterile transfer system further comprises a collection chamber 1, a culture chamber 2 and a cell workstation 3, wherein the collection chamber 1 is connected with the transfer bin gate 46 and is used for collecting samples; the culture chamber 2 is connected with the transfer chamber body 41 and used for carrying out sample culture treatment, and the cell workstation 3 is arranged in the culture chamber 2 and used for completing the operation of directly transferring a sample from the collection chamber 1 to the culture chamber 2 by being connected with the transfer chamber body 41.
In this embodiment, the scanner 42 performs automatic scanning when a sample is placed therein. A PLC control panel is arranged in the controller.
When the sterile transfer system transfers a sample, the controller determines a sample transfer speed parameter A according to the sample mass M and the sample height H, when the determination is finished, the sample transfer speed parameter A is compared with a preset sample transfer speed parameter to determine the initial sample transfer speed, the sample shaking degree D is compared with the preset sample shaking degree to determine the speed regulating quantity of the conveyor belt, the conveyor belt gradient P is compared with the preset conveyor belt gradient to determine a speed correction coefficient and determine a speed correction quantity E through a preset formula, and when the determination is finished, the speed of the conveyor belt is controlled and regulated through the regulating valve;
the sample shaking degree D is obtained by the sample initial image F0 and the sample actual image F.
Specifically, the method comprises the steps of determining a sample transfer speed parameter A according to a sample mass M and a sample height H, comparing the sample transfer speed parameter A with a preset sample transfer speed parameter to determine a sample transfer initial speed when the determination is completed, comparing a sample shaking degree D with a preset sample shaking degree to determine a speed adjustment amount of a conveyor belt, comparing a conveyor belt gradient P with a preset conveyor belt gradient to determine a speed correction coefficient and determining a speed correction amount E through a preset formula, and controlling and adjusting the speed of the conveyor belt through an adjusting valve when the determination is completed, wherein the sample shaking degree D is obtained through a sample initial image F0 and a sample actual image F. Thereby can confirm sample transmission initial velocity through sample quality and sample height to rock degree and conveyer belt slope through the sample and adjust sample transmission velocity, and then can reduce transit time, avoid the sample contaminated, quick convenient, improve work efficiency, moreover, can also reduce the fluctuation of sample in the transmission course, improve the stability of sample.
Specifically, when the sterile transfer system transfers a sample, the controller obtains the detection result of the mass sensor 45 and sets the detection result as the sample mass, meanwhile, the controller analyzes the scanning result of the scanner 42 to obtain the height of the sample and sets the height as the sample height H, when the setting is completed, the controller determines the sample transfer speed parameter a according to the sample mass M and the sample height H,
A＝(M/M0)+(H/H0)；
in the formula, M0 represents a sample annotation mass, and H0 represents a sample standard height.
In this embodiment, the sample annotation mass M0 and the sample standard height H0 are both set by the controller.
Specifically, the sample mass M, the sample height H and a preset formula determine the sample transfer speed parameter A, so that the sample transfer initial speed can be determined through the sample mass and the sample height, and the sample transfer speed is adjusted through the sample shaking degree and the conveyor belt gradient, so that the transfer time can be reduced, the sample is prevented from being polluted, the speed and the convenience are realized, the working efficiency is improved, the fluctuation of the sample in the transfer process can be reduced, and the stability of the sample is improved.
Specifically, when the sample transfer speed parameter a is determined, the controller compares the sample transfer speed parameter a with a preset sample transfer speed parameter to determine a sample transfer initial speed, and when the controller determines that the initial speed of the conveyor belt for transferring the sample is Vi, the controller starts the motor, controls the regulating valve to regulate the conveyor belt speed to Vi, and sets i to 1, 2,3, and 4;
the controller is provided with preset sample transfer speed parameters and a conveyor belt initial speed, wherein the preset sample transfer speed parameters comprise a first preset sample transfer speed parameter A1, a second preset sample transfer speed parameter A2 and a third preset sample transfer speed parameter A3, wherein A1 is more than A2 and is more than A3; the conveyor initial speeds comprise a conveyor first initial speed V1, a conveyor second initial speed V2, a conveyor third initial speed V3 and a conveyor fourth initial speed V4;
if A < A1, the controller determines that the initial speed of the conveyor belt for transferring the sample is V1;
if A1 is not less than A and is less than A2, the controller judges that the initial speed of the conveyor belt for transferring the samples is V2;
if A2 is not less than A and is less than A3, the controller judges that the initial speed of the conveyor belt for transferring the samples is V3;
if A is larger than or equal to A3, the controller judges that the initial speed of the conveyor belt for conveying the sample is V4.
Specifically, the sample transfer initial speed is determined by comparing the sample transfer speed parameter A with a preset sample transfer speed parameter, so that the sample transfer initial speed can be determined according to the sample quality and the sample height, and the sample transfer speed is adjusted according to the sample shaking degree and the conveyor belt gradient, so that the transfer time can be reduced, the sample is prevented from being polluted, the operation is rapid and convenient, the working efficiency is improved, the fluctuation of the sample in the transfer process can be reduced, and the stability of the sample is improved.
Specifically, when the conveyor belt 43 transfers a sample at an initial speed Vi, setting i to 1, 2,3,4, the controller obtains the degree of shake of the sample during the transfer and sets the degree of shake to a sample degree of shake D, when the setting is completed, the controller compares the sample degree of shake D with a preset sample degree of shake to determine a speed adjustment amount of the conveyor belt, and when the controller determines that the speed adjustment amount of the conveyor belt is Qi, the controller controls the adjusting valve to adjust the speed of the conveyor belt, where the adjustment amount is Qi, and sets i to 1, 2,3, 4;
the controller is further provided with preset sample shaking degrees and a conveyor belt speed regulating amount, wherein the preset sample shaking degrees comprise a first preset sample shaking degree D1, a second preset sample shaking degree D2 and a third preset sample shaking degree D3, and D1 is more than D2 and less than D3; the conveyor speed regulating quantity comprises a first conveyor speed regulating quantity Q1, a second conveyor speed regulating quantity Q2, a third conveyor speed regulating quantity Q3 and a fourth conveyor speed regulating quantity Q4, wherein Q1 is more than Q2 and more than Q3 and more than Q4;
if D < D1, the controller determines that the amount of speed adjustment of the conveyor belt is Q1;
if D1 is not less than D < D2, the controller judges that the speed regulating quantity of the conveyor belt is Q2;
if D2 is not less than D < D3, the controller judges that the speed regulating quantity of the conveyor belt is Q3;
if D is larger than or equal to D3, the controller judges that the speed adjustment amount of the conveyor belt is Q4.
Specifically, the speed regulating quantity of the conveyor belt is determined by comparing the sample shaking degree D with the preset sample shaking degree, so that the initial sample transmission speed can be determined according to the sample quality and the sample height, and the sample transmission speed is regulated according to the sample shaking degree and the conveyor belt gradient, so that the transfer time can be reduced, the sample is prevented from being polluted, the operation is fast and convenient, the working efficiency is improved, the fluctuation of the sample in the transmission process can be reduced, and the stability of the sample is improved.
Specifically, when the controller determines that the speed adjustment amount of the conveyor belt is Qi, the controller sets the gradient of the front of the conveyor belt 43, which is to be accessed by the sample, as a conveyor belt gradient P, and when the setting is completed, the controller compares the conveyor belt gradient P with a preset conveyor belt gradient to determine a speed correction coefficient;
the controller is further provided with a preset conveyor belt gradient and a speed correction coefficient, wherein the first preset conveyor belt gradient P1, the second preset conveyor belt gradient P2 and the third preset conveyor belt gradient P3 are included, and P1 is larger than P2 and P3; the speed correction coefficient includes a speed first correction coefficient σ 1, a speed second correction coefficient σ 2, a speed third correction coefficient σ 3, and a speed fourth correction coefficient σ 4, where σ 1 < σ 2 < σ 3 < σ 4 and σ 1+ σ 2+ σ 3+ σ 4 are 2;
if P < P1, the controller determines that the correction coefficient of the speed adjustment amount is σ 1;
if P1 is less than or equal to P < P2, the controller determines that the correction coefficient of the speed adjustment amount is sigma 2;
if P2 is less than or equal to P < P3, the controller determines that the correction coefficient of the speed adjustment amount is sigma 3;
if P is greater than or equal to P3, the controller determines that the correction factor for the speed adjustment amount is σ 4.
In this embodiment, the belt slope is obtained when the belt 43 is set and installed, with data in the controller.
Specifically, the speed correction coefficient is determined by comparing the gradient P of the conveying belt with the preset gradient of the conveying belt, and the speed correction quantity E is determined by the preset formula, wherein the speed correction coefficient aims to improve the accuracy of calculation, so that the initial speed of sample transmission can be determined by the sample quality and the sample height, and the sample transmission speed is adjusted by the sample shaking degree and the gradient of the conveying belt, so that the transfer time can be reduced, the sample is prevented from being polluted, the speed and the convenience are realized, the working efficiency is improved, the fluctuation of the sample in the transmission process can be reduced, and the stability of the sample is improved.
Specifically, when the controller determines that the speed correction coefficient is σ i, i is set to 1, 2,3,4, the controller determines the speed correction amount E,
when i is 1, E is set to Q1 × σ 1;
when i is 2, E is set to Q2 × σ 2;
when i is 3, E is set to Q3 × σ 3;
when i is 4, E is set to Q4 × σ 4.
Specifically, when the controller determines the speed correction amount E, the controller controls the adjustment valve to correct the speed of the conveyor belt, the corrected speed adjustment amount is Q ', Q' is set to Qi ± E, when the gradient of the front of the sample is an upward slope, Q 'is Qi + E, and when the gradient of the front of the sample is a downward slope, Q' is Qi-E, and after the controller corrects the speed adjustment amount, the controller controls the adjustment valve to adjust the speed of the conveyor belt, i is set to 1, 2,3, 4.
Specifically, before the sterile transfer system transfers the sample, the controller acquires an image of the sample collected by the image collector 441 as a sample initial image F0, during the transfer of the sample, the controller acquires the image of the sample collected by the image collector 441 as a sample actual image F in real time, and when the acquisition is completed, the controller compares the sample initial image F0 with the sample actual image F to obtain a sample shaking degree D.
In this embodiment, the image collector 441 may be a camera or other devices, and the present invention is not limited thereto as long as image collection can be achieved.
So far, the technical solutions of the present invention have been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of the present invention is obviously not limited to these specific embodiments. Equivalent changes or substitutions of related technical features can be made by those skilled in the art without departing from the principle of the invention, and the technical scheme after the changes or substitutions can fall into the protection scope of the invention.
Claims (8)
1. An aseptic delivery system based on cell workstation and collection room UNICOM, characterized in that includes:
the transfer bin main body is used for transferring samples;
the scanner is connected with the transfer bin main body and used for scanning the height of the sample to obtain the height H of the sample;
the conveying belt is connected with the conveying bin main body, is arranged in the conveying bin main body and is used for conveying the sample by rotating the conveying belt;
the image collector is connected with the transfer bin main body and is used for collecting a sample image;
the track is connected with the transfer bin main body, is arranged in the transfer bin main body, is provided with an image collector and is used for completing the real-time collection of a sample image by the image collector through the track;
the mass sensor is connected with the conveyor belt, arranged below the conveyor belt and abutted against the conveying bin gate and used for detecting the mass M of the sample;
the motor is connected with the conveyor belt and used for providing power for the conveyor belt;
the adjusting valve is connected with the motor and used for adjusting the speed of the conveyor belt;
the transfer bin door is connected with the transfer bin main body, is used for isolating the interior of the transfer bin from the exterior of the transfer bin, is provided with a door switch, is provided with a torsion, and realizes the opening/closing of the transfer bin door through the torsion;
the sealing strip is connected with the main body of the transfer bin and arranged at the opening of the transfer bin, and the door of the transfer bin is tightly connected with the sealing strip when closed so as to seal the interior of the transfer bin;
the controller is connected with the scanner, the conveyor belt, the image collector, the track, the mass sensor, the motor and the regulating valve and is used for controlling the transmission process of the sample;
when the sterile transfer system transfers a sample, the controller determines a sample transfer speed parameter A according to the sample mass M and the sample height H, when the determination is finished, the sample transfer speed parameter A is compared with a preset sample transfer speed parameter to determine the initial sample transfer speed, the sample shaking degree D is compared with the preset sample shaking degree to determine the speed regulating quantity of the conveyor belt, the conveyor belt gradient P is compared with the preset conveyor belt gradient to determine a speed correction coefficient and determine a speed correction quantity E through a preset formula, and when the determination is finished, the speed of the conveyor belt is controlled and regulated through the regulating valve;
the sample shaking degree D is obtained by the sample initial image F0 and the sample actual image F.
2. The sterile transfer system based on the communication between the cell workstation and the collection room as claimed in claim 1, wherein the controller obtains the detection result of the quality sensor and sets the detection result as the quality of the sample when the sterile transfer system transfers the sample, meanwhile, the controller analyzes the scanning result of the scanner to obtain the height of the sample and sets the height of the sample as the height H of the sample, when the setting is completed, the controller determines the parameter A of the transfer speed of the sample according to the quality M of the sample and the height H of the sample,
A＝(M/M0)+(H/H0)；
in the formula, M0 represents a sample annotation mass, and H0 represents a sample standard height.
3. The aseptic transfer system based on cell workstation and collection chamber communication of claim 2, wherein when the sample transfer speed parameter a is determined to be completed, the controller compares the sample transfer speed parameter a with a preset sample transfer speed parameter to determine a sample transfer initial speed, when the controller determines that the initial speed of the conveyor belt for transferring the sample is Vi, the controller starts the motor, controls the regulating valve to regulate the conveyor belt speed to Vi, and sets i to 1, 2,3, 4;
the controller is provided with preset sample transfer speed parameters and a conveyor belt initial speed, wherein the preset sample transfer speed parameters comprise a first preset sample transfer speed parameter A1, a second preset sample transfer speed parameter A2 and a third preset sample transfer speed parameter A3, wherein A1 is more than A2 and is more than A3; the conveyor initial speeds comprise a conveyor first initial speed V1, a conveyor second initial speed V2, a conveyor third initial speed V3 and a conveyor fourth initial speed V4;
if A < A1, the controller determines that the initial speed of the conveyor belt for transferring the sample is V1;
if A1 is not less than A and is less than A2, the controller judges that the initial speed of the conveyor belt for transferring the samples is V2;
if A2 is not less than A and is less than A3, the controller judges that the initial speed of the conveyor belt for transferring the samples is V3;
if A is larger than or equal to A3, the controller judges that the initial speed of the conveyor belt for conveying the sample is V4.
4. The aseptic transfer system based on the cell workstation and the collection chamber, according to claim 3, wherein when the transfer belt transfers the sample at an initial speed Vi, i is set to 1, 2,3,4, the controller obtains the shaking degree of the sample during the transfer process and sets the shaking degree of the sample as a sample shaking degree D, when the setting is completed, the controller compares the sample shaking degree D with a preset sample shaking degree to determine a speed adjustment amount of the transfer belt, when the controller determines that the speed adjustment amount of the transfer belt is Qi, the controller controls the adjusting valve to adjust the speed of the transfer belt, the adjustment amount is Qi, and i is set to 1, 2,3, 4;
the controller is further provided with preset sample shaking degrees and a conveyor belt speed regulating amount, wherein the preset sample shaking degrees comprise a first preset sample shaking degree D1, a second preset sample shaking degree D2 and a third preset sample shaking degree D3, and D1 is more than D2 and less than D3; the conveyor speed regulating quantity comprises a first conveyor speed regulating quantity Q1, a second conveyor speed regulating quantity Q2, a third conveyor speed regulating quantity Q3 and a fourth conveyor speed regulating quantity Q4, wherein Q1 is more than Q2 and more than Q3 and more than Q4;
if D < D1, the controller determines that the amount of speed adjustment of the conveyor belt is Q1;
if D1 is not less than D < D2, the controller judges that the speed regulating quantity of the conveyor belt is Q2;
if D2 is not less than D < D3, the controller judges that the speed regulating quantity of the conveyor belt is Q3;
if D is larger than or equal to D3, the controller judges that the speed adjustment amount of the conveyor belt is Q4.
5. The cell workstation and collection chamber based sterile transfer system of claim 4 wherein the controller determines that the conveyor belt speed adjustment is Qi, the controller sets the slope of the conveyor belt ahead of the path of the sample to be processed to a conveyor belt slope P, and when the setting is complete, the controller compares the conveyor belt slope P to a preset conveyor belt slope to determine a speed correction factor;
the controller is further provided with a preset conveyor belt gradient and a speed correction coefficient, wherein the first preset conveyor belt gradient P1, the second preset conveyor belt gradient P2 and the third preset conveyor belt gradient P3 are included, and P1 is larger than P2 and P3; the speed correction coefficient includes a speed first correction coefficient σ 1, a speed second correction coefficient σ 2, a speed third correction coefficient σ 3, and a speed fourth correction coefficient σ 4, where σ 1 < σ 2 < σ 3 < σ 4 and σ 1+ σ 2+ σ 3+ σ 4 are 2;
if P < P1, the controller determines that the correction coefficient of the speed adjustment amount is σ 1;
if P1 is less than or equal to P < P2, the controller determines that the correction coefficient of the speed adjustment amount is sigma 2;
if P2 is less than or equal to P < P3, the controller determines that the correction coefficient of the speed adjustment amount is sigma 3;
if P is greater than or equal to P3, the controller determines that the correction factor for the speed adjustment amount is σ 4.
6. The cell workstation-based communication system of claim 5, wherein the controller determines the velocity correction factor as σ i, sets i to 1, 2,3,4, and the controller determines the velocity correction amount E,
when i is 1, E is set to Q1 × σ 1;
when i is 2, E is set to Q2 × σ 2;
when i is 3, E is set to Q3 × σ 3;
when i is 4, E is set to Q4 × σ 4.
7. The system of claim 6, wherein the controller determines a speed correction amount E by controlling the regulating valve to correct the speed of the conveyor belt, wherein the corrected speed correction amount is Q ', Q' Qi ± E is set, Q 'Qi + E is set when the gradient of the front of the sample is an ascending slope, Q' Qi-E is set when the gradient of the front of the sample is a descending slope, and i is 1, 2,3,4 is set when the controller corrects the speed correction amount by controlling the regulating valve to adjust the speed of the conveyor belt.
8. The cell workstation-based sterile transfer system communicated with the collection chamber according to claim 4, wherein before the sterile transfer system transfers the sample, the controller acquires an image of the sample collected by the image collector as a sample initial image F0, during the transfer of the sample, the controller acquires the image of the sample collected by the image collector in real time as a sample actual image F, and when the acquisition is completed, the controller compares the sample initial image F0 with the sample actual image F to obtain a sample shaking degree D.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110702710.9A CN113462563B (en) | 2021-06-24 | 2021-06-24 | Sterile transfer system based on cell workstation and collection room UNICOM |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110702710.9A CN113462563B (en) | 2021-06-24 | 2021-06-24 | Sterile transfer system based on cell workstation and collection room UNICOM |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113462563A true CN113462563A (en) | 2021-10-01 |
| CN113462563B CN113462563B (en) | 2022-12-27 |
Family
ID=77872614
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110702710.9A Active CN113462563B (en) | 2021-06-24 | 2021-06-24 | Sterile transfer system based on cell workstation and collection room UNICOM |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113462563B (en) |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5958336A (en) * | 1996-04-26 | 1999-09-28 | Duarte; Raul | Surface sterilization device |
| CN1453352A (en) * | 2002-04-26 | 2003-11-05 | 叶大林 | Bacteria-free isolated operation system |
| CN101501693A (en) * | 2005-05-16 | 2009-08-05 | 智能医院体系有限公司 | Automated pharmacy admixture system (apas) |
| US20110112684A1 (en) * | 2007-09-06 | 2011-05-12 | Pellenc (Societe Anonyme) | Selective-sorting harvesting machine and sorting chain including one such machine |
| US20140290162A1 (en) * | 2013-03-29 | 2014-10-02 | Shibuya Kogyo Co., Ltd. | Isolator system |
| US20170343993A1 (en) * | 2016-05-27 | 2017-11-30 | Biomerieux, Inc. | System and method of load balancing specimen containers within detection instruments |
| CN109563457A (en) * | 2016-05-27 | 2019-04-02 | 生物梅里埃有限公司 | The system and method for sample container are transported between detecting instrument |
| CN109622075A (en) * | 2019-01-28 | 2019-04-16 | 河南省人民医院 | Oncology air insulated operating room |
| CN109794437A (en) * | 2018-10-30 | 2019-05-24 | 泉州装备制造研究所 | Intelligent sorting system based on computer vision |
| US20190277869A1 (en) * | 2016-07-21 | 2019-09-12 | Siemens Healthcare Diagnostics Inc. | Automated clinical analyzer system and method |
| CN110479388A (en) * | 2019-08-21 | 2019-11-22 | 和泓（厦门）生物技术有限公司 | A kind of sterile operating device for stem cell preparation |
| CN110761594A (en) * | 2019-01-24 | 2020-02-07 | 中船第九设计研究院工程有限公司 | Clinical cell preparation engineering environment barrier system |
| AU2020100599A4 (en) * | 2020-04-19 | 2020-05-28 | Benjamin Lloyd Notley | RiPOD (Rescue Isolation Pod) |
| CN111305614A (en) * | 2020-04-01 | 2020-06-19 | 冰山松洋生物科技(大连)有限公司 | Closed cell tissue and virus modulation system connected by double transfer boxes |
| GB202011780D0 (en) * | 2020-07-29 | 2020-09-09 | Smart4Diagnostics Gmbh | Pre-analytic management of sample container parameters |
-
2021
- 2021-06-24 CN CN202110702710.9A patent/CN113462563B/en active Active
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5958336A (en) * | 1996-04-26 | 1999-09-28 | Duarte; Raul | Surface sterilization device |
| CN1453352A (en) * | 2002-04-26 | 2003-11-05 | 叶大林 | Bacteria-free isolated operation system |
| CN101501693A (en) * | 2005-05-16 | 2009-08-05 | 智能医院体系有限公司 | Automated pharmacy admixture system (apas) |
| US20110112684A1 (en) * | 2007-09-06 | 2011-05-12 | Pellenc (Societe Anonyme) | Selective-sorting harvesting machine and sorting chain including one such machine |
| US20140290162A1 (en) * | 2013-03-29 | 2014-10-02 | Shibuya Kogyo Co., Ltd. | Isolator system |
| CN109563457A (en) * | 2016-05-27 | 2019-04-02 | 生物梅里埃有限公司 | The system and method for sample container are transported between detecting instrument |
| US20170343993A1 (en) * | 2016-05-27 | 2017-11-30 | Biomerieux, Inc. | System and method of load balancing specimen containers within detection instruments |
| US20190277869A1 (en) * | 2016-07-21 | 2019-09-12 | Siemens Healthcare Diagnostics Inc. | Automated clinical analyzer system and method |
| CN109794437A (en) * | 2018-10-30 | 2019-05-24 | 泉州装备制造研究所 | Intelligent sorting system based on computer vision |
| CN110761594A (en) * | 2019-01-24 | 2020-02-07 | 中船第九设计研究院工程有限公司 | Clinical cell preparation engineering environment barrier system |
| CN109622075A (en) * | 2019-01-28 | 2019-04-16 | 河南省人民医院 | Oncology air insulated operating room |
| CN110479388A (en) * | 2019-08-21 | 2019-11-22 | 和泓（厦门）生物技术有限公司 | A kind of sterile operating device for stem cell preparation |
| CN111305614A (en) * | 2020-04-01 | 2020-06-19 | 冰山松洋生物科技(大连)有限公司 | Closed cell tissue and virus modulation system connected by double transfer boxes |
| AU2020100599A4 (en) * | 2020-04-19 | 2020-05-28 | Benjamin Lloyd Notley | RiPOD (Rescue Isolation Pod) |
| GB202011780D0 (en) * | 2020-07-29 | 2020-09-09 | Smart4Diagnostics Gmbh | Pre-analytic management of sample container parameters |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113462563B (en) | 2022-12-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113462563B (en) | Sterile transfer system based on cell workstation and collection room UNICOM | |
| CN103335798A (en) | Soft package lithium battery airtightness detection machine and method | |
| CN108952470B (en) | Intelligent window based on Internet of things computer | |
| WO2021017545A1 (en) | Dust collection station of autonomous cleaning apparatus and dust collection system having same | |
| CN206446816U (en) | A kind of auto-filling weighs closing device | |
| CN217026135U (en) | Single crystal furnace feeding device | |
| CN208579823U (en) | Electronic weighing type coal feeder and its give coal control system | |
| CN208333130U (en) | A kind of melting furnace smoke discharging pipe system | |
| CN218444108U (en) | System for automatically adjusting batching fall difference value | |
| CN213737272U (en) | Covering material automatic control device | |
| CN116588619B (en) | Sterile transfer device for cell detection | |
| CN114515548A (en) | Feeding control device and control method | |
| CN114411241B (en) | Single crystal furnace feeding device | |
| CN207750009U (en) | A kind of automatic seal door | |
| CN216797521U (en) | Cleaning robot for door gap of elevator | |
| CN213678370U (en) | Negative pressure connection pipe for garbage collection terminal | |
| CN217459440U (en) | Low-temperature light radiation box for cell culture | |
| CN110745402A (en) | Regulation type pneumatic valve structure | |
| CN208682125U (en) | A kind of FDM 3D printing device | |
| CN216663079U (en) | Transmission cabin for biological assay | |
| CN215755278U (en) | Integrated automatic powder loading device for foam glass production | |
| CN215466141U (en) | Negative pressure air volume control system and winnowing machine | |
| CN213927135U (en) | Daylighting top photovoltaic smoke discharging window | |
| CN220650543U (en) | Automatic adjust solution pH device | |
| CN215472148U (en) | Concrete mixing plant powder feed arrangement |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |