CN114574352A

CN114574352A - Activity observation device for food microorganism culture experiment and operation method thereof

Info

Publication number: CN114574352A
Application number: CN202210260434.XA
Authority: CN
Inventors: 伊廷存; 孟静; 霍胜楠; 钟立霞; 程祥龙
Original assignee: Shandong Institute for Food and Drug Control
Current assignee: Shandong Institute for Food and Drug Control
Priority date: 2022-03-16
Filing date: 2022-03-16
Publication date: 2022-06-03

Abstract

The invention discloses an activity observation device for a food microorganism culture experiment and an operation method thereof, wherein the activity observation device comprises a base, the top of the base is provided with an incubator, and a cross partition plate is arranged in the incubator; according to the invention, the cross partition plate is arranged in the incubator, the inside of the incubator can be divided into different spaces through the use of the sealing gasket and the sealing groove, the use of the heater and the refrigerator is matched, so that the interiors of the different spaces can be in different mimicry environments, the mimicry environment is more suitable for the growth of microorganisms when workers observe the culture of the microorganisms, the annular groove is formed in the top of the incubator, the annular seat is arranged in the annular groove, and the single-tube electron microscope is arranged in the annular seat, so that the workers can observe the activity of the microorganisms in the different mimicry environments without taking the microorganisms out of the incubator, and the creativity of the device is improved.

Description

Activity observation device for food microorganism culture experiment and operation method thereof

Technical Field

The invention relates to the technical field of microbial culture, in particular to an activity observation device for a food microbial culture experiment and an operation method thereof.

Background

The food microorganism detection is a detection method for detecting the type, quantity and property of microorganisms in food and the influence of the microorganisms on human health by applying the theory and method of microbiology so as to judge whether the food meets the quality standard or not.

The invention discloses a microorganism incubator as the invention patent CN108251277A, which is mainly used for batch culture of microorganisms, and timely supplement of culture solution in a culture tank is realized by utilizing a culture solution conveying pipe, so that the propagation and growth of the microorganisms are improved to the maximum extent; the self-power generation of the stirring rod is utilized to provide the proper temperature in the culture tank; can realize self-sterilization, the sterilization is more thorough, but the device has certain problems when in use, when the device is used for culturing microorganisms in batches, the culture space cannot be divided into different environments, so that the microorganisms cannot be cultured in different mimicry environments, this results in that the growth of the microorganism is not more suitable than that of the microorganism in the mimicry environment during the culture of the microorganism, the creativity is poor, meanwhile, when the device is used for observing the microorganisms, the microorganisms need to be taken out of the culture tank to the outside for observation by using a microscope device, the temperature inside the incubator is different from the outside, so that the microorganism is easily killed due to the stimulation of the outside temperature difference, and the observation of the microorganism by the microscope device fails.

Disclosure of Invention

The invention aims to provide an activity observation device for food microorganism culture experiments and an operation method thereof, and aims to solve the problems that the existing observation device proposed in the background technology cannot be used for dividing different mimicry environments and is inconvenient for direct observation of microorganisms.

In order to achieve the purpose, the invention provides the following technical scheme:

an activity observation device for food microorganism culture experiments comprises a base, wherein an incubator is arranged at the top of the base, a cross partition plate is arranged inside the incubator, a lifting mechanism is arranged on one side of the base, four electric telescopic rods are embedded in the top of the base, a supporting plate is fixedly sleeved at the output end of each electric telescopic rod, a vibrator is arranged at the bottom of the supporting plate, a vessel placing groove is formed in the top of the supporting plate, a heater is arranged at the bottom of the supporting plate, a refrigerator is arranged at the bottom of the supporting plate, the heater and the refrigerator are both arranged at the top of the base, an annular groove is formed in the top of the incubator, an annular seat is arranged inside the annular groove, an annular sleeve is arranged inside the annular seat, a single-tube electron microscope is arranged inside the annular sleeve, and a protective sleeve in threaded connection with the annular sleeve is fixedly sleeved on the surface of the single-tube electron microscope, four electric vent valves are installed at the top of the incubator, and four ultraviolet germicidal lamps are installed at the bottom of the incubator.

Preferably, sealed pad is all installed to the bottom of incubator and the bottom of cross baffle, the seal groove has been seted up at the top of base, sealed pad and seal groove cooperation are used.

Preferably, elevating system includes the fixed block, one side and the base bolt joint of fixed block, the fixed cover of center department of fixed block has connect electric lift rod, the fixed cover of output of electric lift rod has connected the connecting block.

Preferably, the surface of the incubator is fixedly sleeved with a limiting frame, and one side of the connecting block is bolted to the surface of the limiting frame.

Preferably, a temperature sensor is installed at the top of the supporting plate, a temperature controller used in cooperation with the temperature sensor is installed on the front surface of the bottom plate through a fixing piece, and the temperature controller is connected with the heater and the refrigerator through wires.

Preferably, the sliding blocks are installed on the inner surface and the outer surface of the annular seat, the sliding grooves are symmetrically formed in the annular groove, and the sliding blocks are connected with the sliding grooves in a sliding mode.

Preferably, the surface of the annular sleeve is fixedly sleeved with an annular guide block, an annular guide groove is formed in the annular seat, and the annular guide block is connected with the annular guide groove in a sliding mode.

Preferably, the surface of the single-tube electron microscope is provided with a limiting block, the bottom of the limiting block is provided with a limiting insertion rod, and the top of the annular seat is provided with a limiting slot matched with the limiting insertion rod for use.

Preferably, the top of the annular seat is in threaded connection with a discharging pipe, the bottom of the discharging pipe is communicated with a scattering nozzle, the top of the discharging pipe is communicated with a feeding hopper, a plugging block is arranged inside the feeding hopper, and a containing groove matched with the scattering nozzle is formed in the bottom of the annular seat.

An operation method of an activity observation device for food microorganism culture experiments comprises the following steps:

step 1: starting an electric lifting rod in the lifting mechanism, wherein the electric lifting rod drives a limiting frame to move upwards, so that the limiting frame drives the incubator to move upwards;

step 2: the culture dish is placed in the dish placing groove on the supporting plate by the worker, and the electric lifting rod is operated reversely, so that the culture box and the sealing gasket at the bottom of the cross partition plate are clamped into the sealing groove to isolate the external environment;

and step 3: according to the temperature sensors in different spaces, the temperature controller controls the heater and the refrigerator in the space to work and adjust the temperature of different spaces formed by dividing the incubator and the cross partition plate;

and 4, step 4: starting an electric telescopic rod to drive a support plate to move upwards, enabling the support plate to drive a culture dish to ascend to be close to a bulk spray head, rotating a discharging pipe to enable the bulk spray head to be positioned at the top of the culture dish, and adding nutrient solution through a feed hopper;

and 5: culturing microorganisms in a mimicry environment, and ensuring ventilation in the incubator to be adjusted through an electric ventilation valve;

step 6: when the activity of microorganism is observed, only the annular seat inside the annular groove needs to be rotated, so that the annular seat drives the single-tube electron microscope to be in a mimicry space to be observed, the annular sleeve is rotated, the single-tube electron microscope moves towards the inside of the incubator under the matching use of the annular sleeve and the protective sleeve, and the supporting plate moves upwards to drive the culture dish to be close to the single-tube electron microscope, so that the observation can be completed.

Compared with the prior art, the invention provides an activity observation device for food microorganism culture experiments, which has the following beneficial effects:

according to the invention, the cross partition plate is arranged in the incubator, and the inside of the incubator can be divided into different spaces by using the sealing gasket and the sealing groove, so that the interiors of the different spaces can be in different mimicry environments by matching with the heaters and the refrigerators, and the mimicry environments are more suitable for the growth of microorganisms when workers observe the culture of the microorganisms;

according to the invention, the annular groove is formed in the top of the incubator, the annular seat is arranged in the annular groove, and the single-tube electron microscope is arranged in the annular seat, so that a worker can observe the activity of microorganisms in different mimicry environments without taking the microorganisms out of the incubator, and the creativity of the device is improved.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a cross-sectional view of the structure of the present invention;

FIG. 3 is a top view of a portion of the structure of the present invention;

FIG. 4 is a bottom view of a portion of the present invention;

FIG. 5 is an enlarged view of the invention at A in FIG. 2.

In the figure: 1. a base; 2. an incubator; 3. a cross partition plate; 4. a lifting mechanism; 41. a fixed block; 42. an electric lifting rod; 43. connecting blocks; 5. an electric telescopic rod; 6. a support plate; 7. a vibrator; 8. a vessel placing groove; 9. a heater; 10. a refrigerator; 11. an annular groove; 12. an annular seat; 13. an annular sleeve; 14. a single-tube electron microscope; 15. a protective sleeve; 16. an electrically operated vent valve; 17. an ultraviolet germicidal lamp; 18. a gasket; 19. a sealing groove; 20. a limiting frame; 21. a temperature sensor; 22. a temperature controller; 23. a slider; 24. a sliding groove; 25. an annular guide block; 26. an annular guide groove; 27. a limiting block; 28. a limiting inserted rod; 29. limiting slots; 30. a discharging pipe; 31. a spray head; 32. a feed hopper; 33. a chock block; 34. a receiving groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

As shown in fig. 1, fig. 2, fig. 3, fig. 4 and fig. 5, the present invention provides a technical solution: an activity observation device for food microorganism culture experiments comprises a base 1, wherein the top of the base 1 is provided with an incubator 2, a cross-shaped partition plate 3 is arranged inside the incubator 2, one side of the base 1 is provided with a lifting mechanism 4, four electric telescopic rods 5 are embedded in the top of the base 1, the output ends of the electric telescopic rods 5 are fixedly sleeved with a support plate 6, the bottom of the support plate 6 is provided with a vibrator 7, the top of the support plate 6 is provided with a vessel placing groove 8, the bottom of the support plate 6 is provided with a heater 9, the bottom of the support plate 6 is provided with a refrigerator 10, the heater 9 and the refrigerator 10 are both arranged on the top of the base 1, the top of the incubator 2 is provided with an annular groove 11, the inside of the annular groove 11 is provided with an annular seat 12, the inside of the annular seat 12 is provided with an annular sleeve 13, and the inside of the annular sleeve 13 is provided with a single-barrel electron microscope 14, the surface of a single-tube electron microscope 14 is fixedly sleeved with a protective sleeve 15 in threaded connection with an annular sleeve 13, four electric vent valves 16 are installed at the top of an incubator 2, four ultraviolet germicidal lamps 17 are installed at the bottom of the incubator 2, a cross partition plate 3 is installed inside the incubator 2, the inside of the incubator 2 can be divided into different spaces through a sealing gasket 18 and a sealing groove 19, the interiors of the incubators 2 can be in different mimicry environments by matching with a heater 9 and a refrigerator 10, the interiors of the different spaces can be in different mimicry environments, when workers observe microorganism culture, the mimicry environment is more suitable for the growth of microorganisms, an annular groove 11 is formed at the top of the incubator 2, an annular seat 12 is installed inside the annular groove 11, the single-tube electron microscope 14 is installed inside the annular seat 12, and the workers can take out the microorganisms from the inside of the incubator 2 without taking the microorganisms out, the activity of the microorganisms in different mimicry environments can be observed, and the creativity of the device is improved.

Referring to fig. 1 and 2, when the lifting mechanism 4 drives the incubator 2 to cover the top of the base 1, the inside of the incubator 2 is sealed by the sealing groove 19 and the sealing gasket 18, so as to prevent the external temperature from affecting the temperature inside the incubator 2, the sealing gaskets 18 are mounted at the bottom of the incubator 2 and the bottom of the cross partition plate 3, the top of the base 1 is provided with the sealing groove 19, and the sealing gasket 18 and the sealing groove 19 are used in a matching manner.

Referring to fig. 1 and 2, in order to facilitate the lifting of the incubator 2, so as to seal the inner space of the incubator 2 and lift the incubator 2, the culture dish is taken from the inside of the incubator 2, so as to take the microorganism, the lifting mechanism 4 includes a fixing block 41, one side of the fixing block 41 is bolted to the base 1, an electric lifting rod 42 is fixedly sleeved at the center of the fixing block 41, and a connecting block 43 is fixedly sleeved at the output end of the electric lifting rod 42.

Referring to fig. 1 and 2, in order to facilitate the raising of the incubator 2 by the electric lifting rod 42 of the lifting mechanism 4, the surface of the incubator 2 is fixedly sleeved with the limiting frame 20, and one side of the connecting block 43 is bolted to the surface of the limiting frame 20.

Referring to fig. 1, 2 and 3, in order to facilitate the worker to accurately grasp the temperatures in different spaces inside the incubator 2 and to control the temperature changes in different mimicry spaces through the temperature controller 22, the top of the supporting plate 6 is provided with the temperature sensor 21, the front of the bottom plate is provided with the temperature controller 22 used in cooperation with the temperature sensor 21 through the fixing member, and the temperature controller 22 is connected with the heater 9 and the refrigerator 10 through the wires.

Referring to fig. 2 and 5, in order to limit the annular seat 12 conveniently, the annular seat 12 does not fall off when rotating inside the annular groove 11 conveniently, and external factors are prevented from affecting the inner space of the incubator 2, sliding blocks 23 are mounted on the inner surface and the outer surface of the annular seat 12, sliding grooves 24, and the sliding blocks 23 are connected with the sliding grooves 24 in a sliding manner and symmetrically arranged inside the annular groove 11.

Referring to fig. 2 and 5, in order to limit the annular sleeve 13, ensure that the annular sleeve 13 does not displace when rotating, and reduce the friction force when the annular sleeve 13 rotates, an annular guide block 25 is fixedly sleeved on the surface of the annular sleeve 13, an annular guide groove 26 is formed in the annular seat 12, and the annular guide block 25 is slidably connected with the annular guide groove 26.

Referring to fig. 1, 2 and 5, in order to limit the monocular electron microscope 14 and prevent the monocular electron microscope 14 from rotating when the monocular electron microscope 14 moves towards the inner space of the incubator 2, so as to ensure the monocular electron microscope 14 to descend smoothly, a limiting block 27 is installed on the surface of the monocular electron microscope 14, a limiting insertion rod 28 is installed at the bottom of the limiting block 27, and a limiting insertion slot 29 matched with the limiting insertion rod 28 is opened at the top of the annular seat 12.

Referring to fig. 1, 2 and 4, in order to facilitate the addition of nutrient solution into the incubator 2 through the feed hopper 32 by the worker, so as to facilitate the cultivation of the microorganism in the culture dish placed in the vessel placement groove 8, the top of the annular seat 12 is in threaded connection with the feeding pipe 30, the bottom of the feeding pipe 30 is communicated with the scattering nozzle 31, the top of the feeding pipe 30 is communicated with the feed hopper 32, the inside of the feed hopper 32 is provided with the plug 33, and the bottom of the annular seat 12 is provided with the accommodating groove 34 used in cooperation with the scattering nozzle 31.

An operation method of an activity observation device for food microorganism culture experiments comprises the steps of starting an electric lifting rod 42 in a lifting mechanism 4, driving a limit frame 20 to move upwards by the electric lifting rod 42, driving an incubator 2 to move upwards by the limit frame 20, enabling a worker to place a culture dish in a dish placing groove 8 on a support plate 6, reversely operating the electric lifting rod 42, enabling sealing gaskets 18 at the bottoms of the incubator 2 and a cross partition plate 3 to be clamped into a sealing groove 19 to isolate the external environment, controlling a heater 9 and a refrigerator 10 in different spaces to work according to temperature sensors 21 in the different spaces by a temperature controller 22 when the temperature of the different spaces formed by dividing the incubator 2 and the cross partition plate 3 needs to be adjusted, enabling the simulated environment temperatures in the different spaces to be adjusted, and enabling the microorganisms in the culture dish to be added with nutrient substances, only the electric telescopic rod 5 is started to drive the support plate 6 to move upwards, the support plate 6 drives the culture dish to ascend to be close to the dispersion nozzle 31, the blanking pipe 30 is rotated to enable the dispersion nozzle 31 to be positioned at the top of the culture dish, nutrient solution is added through the feed hopper 32, ventilation inside the culture box 2 is ensured through the electric ventilation valve 16, and therefore the purpose of culturing microorganisms in different mimicry environments is achieved, when activity of the microorganisms needs to be observed, only the annular seat 12 inside the annular groove 11 needs to be rotated, the annular seat 12 drives the monocular electron microscope 14 to be positioned in a mimicry space needing to be observed, the annular sleeve 13 is rotated, the monocular electron microscope 14 moves towards the inside of the culture box 2 under the matching use of the annular sleeve 13 and the protective sleeve 15, the support plate 6 is moved upwards to drive the culture dish to be close to the monocular electron microscope 14, and the operation steps are as above, can reach the microorganism of observing the culture dish inside like this to need not to take out culture dish from incubator 2 is inside, guarantee microbial growth.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in the process, method, article, or apparatus that comprises the element.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. An activity viewing device for food microorganism culture experiments comprises a base (1), and is characterized in that: the incubator is characterized in that an incubator (2) is arranged at the top of the base (1), a cross partition plate (3) is arranged inside the incubator (2), a lifting mechanism (4) is arranged on one side of the base (1), four electric telescopic rods (5) are embedded into the top of the base (1), a supporting plate (6) is fixedly sleeved at the output end of each electric telescopic rod (5), a vibrator (7) is installed at the bottom of the supporting plate (6), a vessel placing groove (8) is formed in the top of the supporting plate (6), a heater (9) is arranged at the bottom of the supporting plate (6), a refrigerator (10) is arranged at the bottom of the supporting plate (6), the heater (9) and the refrigerator (10) are both installed at the top of the base (1), an annular groove (11) is formed in the top of the incubator (2), an annular seat (12) is arranged inside the annular groove (11), the inside of annular seat (12) is provided with annular sleeve (13), the inside of annular sleeve (13) is provided with single-tube electron microscope (14), the fixed surface of single-tube electron microscope (14) cup joints the lag tube (15) with annular sleeve (13) threaded connection, four electronic breather valves (16) are installed at the top of incubator (2), four sterilamp (17) are installed to the bottom of incubator (2).

2. The apparatus for observing the activity of claim 1, wherein: sealing gasket (18) are all installed to the bottom of incubator (2) and the bottom of cross baffle (3), seal groove (19) have been seted up at the top of base (1), sealing gasket (18) and seal groove (19) cooperation are used.

3. The apparatus for observing the activity of claim 1, wherein: elevating system (4) are including fixed block (41), one side and base (1) bolt joint of fixed block (41), the fixed electric lift pole (42) that has cup jointed in center department of fixed block (41), the fixed cover of output of electric lift pole (42) has connect connecting block (43).

4. The apparatus for observing activity in culture experiment of microorganisms in food according to claim 3, wherein: the surface of the incubator (2) is fixedly sleeved with a limiting frame (20), and one side of the connecting block (43) is bolted to the surface of the limiting frame (20).

5. The apparatus for observing the activity of claim 1, wherein: temperature sensor (21) are installed at the top of backup pad (6), the front of bottom plate is installed through the mounting and is used with temperature sensor (21) cooperation temperature controller (22), temperature controller (22) are connected with heater (9) and refrigerator (10) through the wire.

6. The apparatus for observing the activity of claim 1, wherein: sliding blocks (23) are installed on the inner surface and the outer surface of the annular seat (12), sliding grooves (24) are symmetrically formed in the inner portion of the annular groove (11), and the sliding blocks (23) are connected with the sliding grooves (24) in a sliding mode.

7. The apparatus for observing the activity of claim 1, wherein: the surface of the annular sleeve (13) is fixedly sleeved with an annular guide block (25), an annular guide groove (26) is formed in the annular seat (12), and the annular guide block (25) is connected with the annular guide groove (26) in a sliding mode.

8. The activity observation device for the food microorganism culture experiment according to claim 1, wherein: the surface mounting of single-tube electron microscope (14) has stopper (27), spacing inserted bar (28) are installed to the bottom of stopper (27), spacing slot (29) that use with spacing inserted bar (28) cooperation are seted up at the top of annular seat (12).

9. The apparatus for observing the activity of claim 1, wherein: the top threaded connection of annular seat (12) has unloading pipe (30), the bottom intercommunication of unloading pipe (30) has scattered shower nozzle (31), the top intercommunication of unloading pipe (30) has feeder hopper (32), the internally mounted of feeder hopper (32) has chock (33), the bottom of annular seat (12) is seted up and is accomodate groove (34) with scattered shower nozzle (31) cooperation use.

10. An operation method of an activity observation device for food microorganism culture experiments comprises the following steps:

step 1: starting an electric lifting rod (42) in the lifting mechanism (4), wherein the electric lifting rod (42) drives the limiting frame (20) to move upwards, so that the limiting frame (20) drives the incubator (2) to move upwards;

step 2: the culture dish is placed in the dish placing groove (8) on the supporting plate (6) by a worker, and the electric lifting rod (42) is operated reversely, so that the culture box (2) and the sealing gasket (18) at the bottom of the cross partition plate (3) are clamped into the sealing groove (19) to isolate the external environment;

and 3, step 3: according to temperature sensors (21) in different spaces, a temperature controller (22) is used for controlling a heater (9) and a refrigerator (10) in the space to work and adjust the temperature of the different spaces formed by dividing the incubator (2) and the cross partition plate (3);

and 4, step 4: starting the electric telescopic rod (5) to drive the supporting plate (6) to move upwards, enabling the supporting plate (6) to drive the culture dish to ascend to be close to the scattered spray head (31), rotating the discharging pipe (30) to enable the scattered spray head (31) to be located at the top of the culture dish, and adding nutrient solution through the feeding hopper (32);

and 5: culturing microorganisms in a mimicry environment, and ensuring ventilation inside the incubator (2) to be adjusted through an electric ventilation valve (16);

and 6: when the activity of microorganism is observed, only the annular seat (12) inside the annular groove (11) needs to be rotated, the annular seat (12) drives the single-tube electron microscope (14) to be in a mimicry space needing to be observed, the annular sleeve (13) is rotated, the annular sleeve (13) and the protective sleeve (15) are matched for use, the single-tube electron microscope (14) moves towards the inside of the incubator (2), the support plate (6) moves upwards to drive the culture dish to be close to the single-tube electron microscope (14), and the observation can be completed.