CN113699022B - Microorganism culture device and culture method thereof - Google Patents

Microorganism culture device and culture method thereof Download PDF

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Publication number
CN113699022B
CN113699022B CN202110938600.2A CN202110938600A CN113699022B CN 113699022 B CN113699022 B CN 113699022B CN 202110938600 A CN202110938600 A CN 202110938600A CN 113699022 B CN113699022 B CN 113699022B
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casing
face
bearing
shell
pipe
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CN113699022A (en
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王寒微
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Shanghai Zhichu Instrument Co ltd
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Shanghai Zhichu Instrument Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/40—Manifolds; Distribution pieces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18—Flow directing inserts
    • C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20—Degassing; Venting; Bubble traps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the field of microorganism culture, in particular to a microorganism culture device, which comprises a shell, wherein a driving piece is arranged on the lower end face of the shell in a penetrating way, a bearing rod is arranged on the outer side of the driving piece, a heating lamp is fixedly connected to the side face of the bearing rod, a guide piece is arranged between the inner part of the shell and the bearing rod, a liquid inlet pipe is arranged in a penetrating and rotating way in the middle of the upper end face of the shell, a multicomponent liquid pipe is fixedly communicated with the annular array of the outer surface of the liquid inlet pipe, a nozzle is fixedly communicated with the end part of the liquid separation pipe, a blocking housing is arranged on the outer side of the nozzle, and a liquid adding groove disc is arranged on the lower end face of the blocking housing in a penetrating way.

Description

Microorganism culture device and culture method thereof
Technical Field
The invention relates to the field of microorganism culture, in particular to a microorganism culture device and a microorganism culture method.
Background
Microorganisms are a collective term for all microorganisms that are difficult for an individual to observe with the naked eye. Microorganisms including bacteria, viruses, fungi, few algae and the like can be clearly seen through a microscope which is enlarged by 50-300 times, in the prior art, when microorganisms in foods are cultivated through a microorganism cultivation device, the microorganisms are easily damaged when the culture solution is sprayed on the foods due to overlarge culture solution pressure sprayed through a nozzle, and when the cultivation temperature is controlled through a heating lamp, the foods adjacent to the heating lamp are overheated due to long-time heating due to the fact that the heating lamp is in a fixed state, so that the phenomenon of microorganism cultivation is affected.
Disclosure of Invention
The invention aims to solve the defects in the background art and provides a microorganism culture device and a culture method thereof.
In order to achieve the above purpose, the invention adopts the following technical scheme: the utility model provides a microorganism culture apparatus, includes the casing, the lower terminal surface of casing runs through and installs the driving piece, the carrier bar is installed in the outside of driving piece, the side fixedly connected with heating lamp of carrier bar, install the guide between the inside of casing and the carrier bar, the up end middle part of casing runs through and rotates and inlay has the feed liquor pipe, the surface annular array of feed liquor pipe is fixed to be linked together and is had the multicomponent liquid pipe, the tip fixed intercommunication of divide the liquid pipe has the nozzle, the outside of nozzle is provided with blocks the housing, it has the liquid feeding trough dish to block the lower terminal surface of housing to inlay, the support article is installed in the outside of feed liquor pipe, the support article is located the below of adding the liquid trough dish, the side of casing runs through and installs the exhaust part.
Preferably, the inside top surface fixed mounting of casing has the temperature controller, the lower terminal surface of casing extends there is the shell seat, the preceding terminal surface of casing rotates installs sealing door, sealing door's preceding terminal surface runs through and inlays has transparent glass, the driving piece is including running through the drive shaft that rotates the lower terminal surface middle part of inlaying at the casing, the coaxial fixed mounting of lower tip of drive shaft has servo positive and negative motor, servo positive and negative motor is connected with the shell seat, the drive shaft is connected with the carrier bar.
Preferably, the motor frame has been inlayed to the side of shell seat, the tip of motor frame and servo positive and negative rotation motor's side fixed connection, the surface annular array of drive shaft extends there are three link sets, the surface annular array slope of drive shaft extends there are three reinforcing frame sets, the reinforcing frame is located the link under, the tip of link and the tip of reinforcing frame all with the surface fixed connection of carrier bar, the outside coaxial inlay of feed liquor pipe has the rolling disc, the rolling disc is located the top of casing.
Preferably, the guide piece comprises a guide circular ring embedded on the inner outer surface of the shell, a connecting wheel shaft extends from the front end surface of the bearing rod, a guide wheel is rotatably arranged on the outer side of the connecting wheel shaft, and the guide wheel is attached to the outer surface of the guide circular ring.
Preferably, the inside surface of liquid feeding groove dish is inlayed and is had the linking frame, the tip fixed mounting of linking frame has the dispersion post, the conical surface is seted up to the up end of dispersion post, the axial lead of dispersion post and the axial lead of nozzle are located same plumb line, liquid feeding groove dish and the integrated into one piece that blocks the housing, it has the spliced pole to block the inside surface upper edge extension of housing, the tip and the surface fixed connection of nozzle of spliced pole.
Preferably, the support article comprises a bearing support ring fixedly arranged on the outer side of the liquid inlet pipe, a plurality of groups of support trays are annularly arranged on the upper end face of the bearing support ring in an array mode, the support trays are located under the liquid adding groove tray, and a plurality of groups of round holes are formed in the inner bottom face of the liquid adding groove tray in a penetrating mode.
Preferably, the exhaust piece is including running through the intake pipe of inlaying in one side upper portion of casing, the opposite side upper portion of casing runs through and inlays there is the blast pipe, the coaxial extension of tip of blast pipe has the carrier groove ring, the cross-sectional shape of carrier groove ring is L shape, the filter screen is installed in the laminating of carrier groove ring's inside bottom surface, the up end of intake pipe and the up end of blast pipe all run through and install the control valve, the side of carrier groove ring is run through and is seted up and stir the recess.
Preferably, the bearing groove ring and the exhaust pipe are integrally formed, four groups of bearing frame bodies extend from an annular array of the outer surface of the bearing groove ring, limiting sliding grooves are formed in the upper end face and the lower end face of the bearing frame bodies in a penetrating mode, pressing blocks are mounted on the inner front end face of the bearing frame bodies in a penetrating mode, four groups of through holes are formed in the annular array of the outer surface of the bearing groove ring in a penetrating mode, the bearing frame bodies are located on the sides of the through holes, the pressing blocks are attached to the inner portions of the through holes, inclined faces are formed in the edges of the outer surface of one side of the pressing blocks, the outer surface of the other side of the pressing blocks is tightly pressed to the side face of the filter screen, limiting sliding blocks extend from the upper end face and the lower end face of the pressing blocks, the pressing blocks and the limiting sliding blocks are integrally formed, the limiting sliding blocks are attached to the inner portions of the limiting sliding grooves, and spring bodies extend from the front end faces of the inner portions of the bearing frame bodies, and the end portions of the spring bodies are fixedly connected with the rear end faces of the pressing blocks.
Also provided is a culture method of the microorganism culture apparatus, comprising the steps of:
s1: placing a tray filled with food on the support object, then injecting the culture solution through a liquid inlet pipe, and sprinkling the culture solution on the food through a liquid adding tank tray;
s2: then the interior of the shell is heated by the heating lamp, and the driving piece drives the heating lamp to rotate for heating, so that the temperature in the shell reaches the culture temperature for culture;
s3: and open blast pipe and intake pipe regularly, the waste gas that produces when making the cultivation in the casing can be discharged, and oxygen gets into in the casing through the intake pipe simultaneously to guarantee good air quality in the casing, make cultivation can normally go on.
Compared with the prior art, the invention has the following beneficial effects:
1. can drive the carrier bar under the drive of pivoted drive shaft and rotate, guide wheel on the carrier bar rolls on the guide ring in order to assist the carrier bar to rotate round the axle center of guide ring this moment, and then drive the heating lamp on the carrier bar and rotate around the guide ring and heat, in order to avoid when heating in to the casing through the heating lamp, food adjacent with the heating lamp can cause the overheated phenomenon that influences microorganism to cultivate of food because of being heated for a long time, with this cultivation effect that improves, simultaneously under the effect of the multicomponent liquid pipe of annular array, can divide the interior culture solution of feed liquor pipe and export and fall on the tray in the effect of the multiunit round hole of liquid feeding trough bottom, with this food on the assurance tray can fully cultivate with the contact of culture liquid phase, with further improvement its cultivation effect.
2. The culture solution sprayed out through the nozzle can directly strike on the dispersion column to disperse and strike the sprayed culture solution on the inner wall of the blocking housing and the liquid adding groove disk through the dispersion column, so that the culture solution can fall down along the inner wall of the blocking housing and the liquid adding groove disk and be discharged out of the food through the round hole at the bottom of the liquid adding groove disk, thereby avoiding the phenomenon of food damage caused by overhigh culture solution pressure sprayed out from the nozzle.
3. Through directly stirring the restriction slider, can order about the restriction slider and slide in the restriction spout to in driving the fixed press block that is promoted by the spring body and removing the through-hole, make the filter screen that is compressed tightly by the press block can be movable, the filter screen of pulling later, in order to take out the filter screen from bearing the weight of the inslot and can maintain the change, with this effectual follow-up maintenance change of having made things convenient for, improved convenient for use.
Drawings
FIG. 1 is a schematic diagram showing the structure of a microorganism culture apparatus according to the present invention;
FIG. 2 is an internal view of a housing of a microorganism culture apparatus according to the invention;
FIG. 3 is a partial view of the interior of a housing of a microorganism culture apparatus according to the invention;
FIG. 4 is a cross-sectional view of a barrier housing of a microbial culture apparatus according to the present invention;
FIG. 5 is a schematic view of an exhaust pipe of a microorganism culture apparatus according to the present invention;
FIG. 6 is a schematic diagram of a microorganism culture apparatus according to the invention at a press block.
In the figure: 1. a housing; 2. sealing the door; 3. an exhaust pipe; 4. an air inlet pipe; 5. a housing base; 6. a rotating disc; 7. a liquid inlet pipe; 8. a load-bearing slotted ring; 9. a guide ring; 10. a heating lamp; 11. a blocking cover; 12. a servo forward and reverse rotation motor; 13. a motor frame; 14. a connecting frame; 15. a reinforcing frame; 16. a drive shaft; 17. a liquid separating pipe; 18. a nozzle; 19. a liquid adding tank plate; 20. a carrier bar; 21. connecting a wheel shaft; 22. a guide wheel; 23. a bearing support ring; 24. a tray; 25. a dispersion column; 26. a connecting frame; 27. a filter screen; 28. a cladding block; 29. a control valve; 30. limiting the chute; 31. a limit slider; 32. a carrying frame; 33. a spring body; 34. toggle the groove; 35. and a through hole.
Description of the embodiments
The following description is presented to enable one of ordinary skill in the art to make and use the invention. The preferred embodiments in the following description are by way of example only and other obvious variations will occur to those skilled in the art.
The utility model provides a microorganism culture apparatus as shown in fig. 1-6, including casing 1, the lower terminal surface of casing 1 runs through and installs the driving piece, carrier bar 20 is installed in the outside of driving piece, the side fixedly connected with heating lamp 10 of carrier bar 20, install the guide between the inside of casing 1 and the carrier bar 20, the up end middle part of casing 1 runs through and rotates and inlay and have feed liquor pipe 7, the surface annular array of feed liquor pipe 7 fixedly communicates and has multicomponent liquid pipe 17, the tip fixedly communicates of feed liquor pipe 17 has nozzle 18, the outside of nozzle 18 is provided with and blocks housing 11, the lower terminal surface of blocking housing 11 is inlayed and is had liquid feeding groove dish 19, the support article is installed in the outside of feed liquor pipe 7, support article is located the below of liquid feeding groove dish 19, the side of casing 1 runs through and installs the exhaust piece, can lead out the culture solution branch in the feed liquor pipe 7 into nozzle 18 through the feed liquor pipe 17 that sets up, the effect of heating lamp 10 plays the effect of heating, play the temperature controller of accuse temperature, control heating lamp 10 stop work after the temperature reaches.
The inside top surface fixed mounting of casing 1 has the temperature controller, the lower terminal surface of casing 1 extends there is casing seat 5, sealing door 2 is installed in the preceding terminal surface rotation of casing 1, sealing door 2's preceding terminal surface runs through and inlays there is transparent glass, the driving piece is including running through the drive shaft 16 that rotates the lower terminal surface middle part of inlaying at casing 1, the coaxial fixed mounting of lower tip of drive shaft 16 has servo positive and negative motor 12, servo positive and negative motor 12 is connected with casing seat 5, drive shaft 16 is connected with carrier bar 20, can support casing 1 through the casing seat 5 that sets up, sealing door 2 plays the effect to casing 1, servo positive and negative motor 12 can drive shaft 16 and carry out forward and reverse rotation, carry out reverse rotation 120 degrees after the heat lamp 10 on the carrier bar 20 is rotated 120 degrees promptly, with this circulation.
The motor frame 13 has been inlayed to the side of shell seat 5, the tip of motor frame 13 and servo positive and negative rotation motor 12's side fixed connection, the surface annular array of drive shaft 16 extends there are three link 14, the surface annular array of drive shaft 16 extends there are three sets of enhancement shelves 15, enhancement shelf 15 is located the link 14 under, the tip of link 14 and the tip of enhancement shelf 15 all with the surface fixed connection of carrier bar 20, the coaxial rolling disc 6 of inlaying in the outside of feed liquor pipe 7, the rolling disc 6 is located the top of casing 1, can link together carrier bar 20 and drive shaft 16 through the link 14 that sets up, enhancement shelf 15 can strengthen the junction of carrier bar 20 and drive shaft 16, motor frame 13 plays the effect of fixing servo positive and negative rotation motor 12.
The guide piece includes the guide ring 9 of inlaying at the inside surface of casing 1, the preceding terminal surface of carrier bar 20 extends there is connecting axle 21, the outside rotation of connecting axle 21 installs guide wheel 22, guide wheel 22 laminating is in the surface of guide ring 9, can prop up guide wheel 22 through the guide ring 9 that sets up, connecting axle 21 plays the effect of bearing to guide wheel 22, guide wheel 22 plays the effect of guide, prop up the support to reduce the bearing capacity of carrier bar 20.
The inside surface of adding the cistern dish 19 is inlayed and is had linking frame 26, linking frame 26's tip fixed mounting has dispersion post 25, the conical surface is seted up to dispersion post 25's up end, dispersion post 25's axial lead and nozzle 18's axial lead are located same plumb line, it has the spliced pole to add cistern dish 19 and the outer surface fixed connection of nozzle 18 to block the inside surface upper edge department extension of housing 11, the tip of spliced pole can fix dispersion post 25 through the linking frame 26 that sets up, dispersion post 25 of conical surface is seted up to the up end can disperse the culture solution, it plays the effect that blocks the culture solution of dispersion to block housing 11, the spliced pole plays the effect that will block housing 11 and nozzle 18 to link together.
The support article includes the bearing support ring 23 of fixed mounting in the outside of feed liquor pipe 7, bearing support ring 23's up end annular array extends has multiunit tray 24, tray 24 is located under the liquid adding groove dish 19, the inside bottom surface of liquid adding groove dish 19 runs through and has seted up multiunit round hole, can fix tray 24 through bearing support ring 23 that sets up, tray 24 can place the tray that is equipped with food, the inside bottom surface of liquid adding groove dish 19 runs through and has seted up multiunit round hole, can make the even whereabouts of the culture solution in the liquid adding groove dish 19 on the food.
The exhaust piece is including running through the intake pipe 4 of inlaying in one side upper portion of casing 1, the opposite side upper portion of casing 1 runs through and inlays there is blast pipe 3, the coaxial extension of tip of blast pipe 3 has the carrier groove ring 8, the cross-sectional shape of carrier groove ring 8 is L shape, the inside bottom surface laminating of carrier groove ring 8 is installed filter screen 27, control valve 29 is all run through to the up end of intake pipe 4 and blast pipe 3, stir recess 34 has been run through to the side of carrier groove ring 8, can be with the leading-in casing 1 of oxygen in through the intake pipe 4 that sets up, can keep the abundant state of oxygen in the casing 1 when making cultivate, blast pipe 3 can be with the exhaust gas discharge who produces when cultivateing, filter screen 27 plays the effect of purifying waste gas, by activated carbon makes, control valve 29 plays the effect of opening and close intake pipe 4 and blast pipe 3, stir recess 34 can be convenient for pull out filter screen 27.
The bearing groove ring 8 and the blast pipe 3 integrated into one piece, the surface annular array of bearing groove ring 8 extends there are four groups of bearing frame bodies 32, the restriction spout 30 has all been run through to the up and down terminal surface of bearing frame body 32, the inside laminating of bearing frame body 32 is installed and is pressed covering piece 28, four groups of through-holes 35 have been run through to the surface annular array of bearing groove ring 8, bearing frame body 32 is located the side of through-hole 35, press covering piece 28 laminating in the inside of through-hole 35, one side surface edge of press covering piece 28 is offered to the inclined plane, the opposite side surface of press covering piece 28 compresses tightly in the side of filter screen 27, the up and down terminal surface of press covering piece 28 all extends there is restriction slider 31, press covering piece 28 and restriction slider 31 integrated into one piece, restriction slider 31 laminating is in the inside of restriction spout 30, the inside front end surface of bearing frame body 32 extends there is spring body 33, the tip of spring body 33 and the rear end fixed connection of press covering piece 28, press covering piece 28 can be restricted through the bearing frame body 32 that sets up, press covering piece 28 can fix filter screen 27, restriction slider 31 and restriction spout 30 can guarantee to promote press covering piece 28 to be moved to the straight line and press covering piece 28 to fix filter screen 27.
Also provided is a culture method of the microorganism culture apparatus, comprising the steps of:
s1: placing a tray 24 containing food on the support, then injecting the culture solution through the liquid inlet pipe 7, and sprinkling the culture solution on the food through the liquid adding groove plate 19;
s2: then the heating lamp 10 heats the inside of the shell 1, and the driving piece drives the heating lamp 10 to rotate for heating, so that the temperature in the shell 1 reaches the culture temperature for culture;
s3: and the exhaust pipe 3 and the air inlet pipe 4 are opened regularly, so that waste gas generated during cultivation in the casing 1 can be discharged, and meanwhile, oxygen enters the casing 1 through the air inlet pipe 4, so that good air quality in the casing 1 is ensured, and cultivation can be performed normally.
During operation, the tray filled with food is placed in the tray 24, then the culture solution is injected through the liquid inlet pipe 7, the culture solution enters the nozzle 18 and is sprayed out under the guidance of the liquid distribution pipe 17, the culture solution sprayed out through the nozzle 18 can directly collide on the dispersing column 25, so that the sprayed culture solution is dispersed and impacted on the blocking housing 11 and the inner wall of the liquid adding groove disc 19 through the dispersing column 25, the culture solution can fall along the blocking housing 11 and the inner wall of the liquid adding groove disc 19 and is discharged out of the falling food through the round hole at the bottom of the liquid adding groove disc 19, meanwhile, the heating lamp 10 is operated to heat, so that the temperature suitable for culture is reached in the shell 1, at this time, the servo positive and negative rotating motor 12 is operated to drive the driving shaft 16 to rotate, and then the guide wheel 22 on the driving shaft 16 is driven to roll on the guide ring 9 to assist the bearing rod 20 to rotate around the axis of the guide ring 9, and then the heating lamp 10 on the bearing rod 20 is driven to rotate around the guide ring 9 to heat the culture solution, meanwhile, the heating lamp 10 is operated to heat the inner wall of the blocking housing 11 and the air inlet pipe 1 is heated, the air inlet pipe 1 can be opened regularly, and the air inlet pipe 1 can be air can be discharged into the shell 1, and the air inlet pipe 1 can be normally and air can be normally discharged in the shell 1.
In summary, the carrier bar 20 can be driven by the rotating driving shaft 16 to rotate, at this time, the guiding wheel 22 on the carrier bar 20 rolls on the guiding ring 9 to assist the carrier bar 20 to rotate around the axis of the guiding ring 9, so as to drive the heating lamp 10 on the carrier bar 20 to rotate around the guiding ring 9 for heating, so as to avoid the phenomenon that the food adjacent to the heating lamp 10 will overheat and affect the microorganism culture due to long-time heating when the heating lamp 10 heats the casing 1, thereby improving the culture effect, and meanwhile, under the action of the multicomponent liquid tube 17 of the annular array, the culture liquid in the liquid inlet tube 7 can be guided out and falls on the tray 24 under the action of a plurality of groups of round holes at the bottom of the liquid feeding trough tray 19, thereby ensuring that the food on the tray 24 can be fully contacted with the culture liquid for culture, further improving the culture effect; the culture solution sprayed by the nozzle 18 directly impacts the dispersion column 25 so as to disperse and impact the sprayed culture solution on the inner walls of the blocking housing 11 and the liquid adding groove disc 19 through the dispersion column 25, so that the culture solution can fall along the inner walls of the blocking housing 11 and the liquid adding groove disc 19 and be discharged onto the falling food through the round hole at the bottom of the liquid adding groove disc 19, thereby avoiding the phenomenon of food damage caused by overhigh culture solution pressure sprayed from the nozzle 18; through directly stirring restriction slider 31, can order about restriction slider 31 and slide in restriction spout 30 to drive by the fixed press cover piece 28 of spring body 33 promotion and remove and break away from in the through-hole 35, make the filter screen 27 that is pressed by press cover piece 28 can be movable, later pulling filter screen 27, with filter screen 27 take out from bearing groove ring 8 in can maintain the change, with this effectual follow-up maintenance change of having made things convenient for, improved convenient for degree of use.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made therein without departing from the spirit and scope of the invention, which is defined by the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (7)

1. A microorganism culture apparatus comprising a housing (1), characterized in that: the device comprises a shell (1), wherein a driving piece is arranged on the lower end face of the shell (1) in a penetrating mode, a bearing rod (20) is arranged on the outer side of the driving piece, a heating lamp (10) is fixedly connected to the side face of the bearing rod (20), a guide piece is arranged between the inner part of the shell (1) and the bearing rod (20), a liquid inlet pipe (7) is arranged in the middle of the upper end face of the shell (1) in a penetrating mode, multicomponent liquid pipes (17) are fixedly communicated with the annular array of the outer surface of the liquid inlet pipe (7), a nozzle (18) is fixedly communicated with the end portion of the liquid distribution pipe (17), a blocking housing (11) is arranged on the outer side of the nozzle (18), a liquid adding groove disc (19) is arranged on the lower end face of the blocking housing (11) in a penetrating mode, a supporting object is arranged on the outer side face of the liquid inlet pipe (7), the supporting object is located below the liquid adding groove disc (19), and an exhaust piece is arranged on the side face of the shell (1) in a penetrating mode.
The device is characterized in that a connecting frame (26) is inlaid on the inner outer surface of the liquid adding groove disc (19), a dispersing column (25) is fixedly arranged at the end part of the connecting frame (26), the upper end surface of the dispersing column (25) is provided with a conical surface, the axial lead of the dispersing column (25) and the axial lead of the nozzle (18) are positioned on the same vertical line, the liquid adding groove disc (19) and the blocking housing (11) are integrally formed, a connecting column extends at the upper edge of the inner outer surface of the blocking housing (11), and the end part of the connecting column is fixedly connected with the outer surface of the nozzle (18);
the support article comprises a bearing support ring (23) fixedly arranged on the outer side of the liquid inlet pipe (7), a plurality of groups of support trays (24) are annularly arranged on the upper end face of the bearing support ring (23) in an extending mode, the support trays (24) are located under the liquid adding groove disc (19), and a plurality of groups of round holes are formed in the inner bottom face of the liquid adding groove disc (19) in a penetrating mode.
2. A microbial culture apparatus according to claim 1, wherein: the utility model discloses a motor drive device for a motor vehicle, including casing (1), sealed door (2) are installed in the preceding terminal surface rotation of casing (1), sealed door (2) are run through and are inlayed transparent glass, the driving piece is including running through drive shaft (16) at the lower terminal surface middle part of casing (1) that rotate, the coaxial fixed mounting of lower tip of drive shaft (16) has servo positive reverse motor (12), servo positive reverse motor (12) are connected with casing (5), drive shaft (16) are connected with carrier bar (20).
3. A microbial culture apparatus according to claim 2, wherein: the motor frame (13) is inlayed to the side of shell seat (5), the tip of motor frame (13) and the side fixed connection of servo positive and negative rotation motor (12), the surface annular array of drive shaft (16) extends there are three link (14), the surface annular array slope of drive shaft (16) extends there are three reinforcement frame (15) of group, reinforcement frame (15) are located under link (14), the tip of link (14) and the tip of reinforcement frame (15) all with the surface fixed connection of carrier bar (20), coaxial inlayed in the outside of feed liquor pipe (7) has rolling disc (6), rolling disc (6) are located the top of casing (1).
4. A microbial culture apparatus according to claim 1, wherein: the guide piece comprises a guide circular ring (9) inlaid on the inner outer surface of the shell (1), a connecting wheel shaft (21) extends from the front end surface of the bearing rod (20), a guide wheel (22) is rotatably arranged on the outer side of the connecting wheel shaft (21), and the guide wheel (22) is attached to the outer surface of the guide circular ring (9).
5. A microbial culture apparatus according to claim 1, wherein: the exhaust part is including running through the intake pipe (4) of inlaying on one side upper portion of casing (1), the opposite side upper portion of casing (1) runs through and inlays has blast pipe (3), the coaxial extension of tip of blast pipe (3) has to bear groove ring (8), the cross-sectional shape that bears groove ring (8) is L shape, filter screen (27) are installed in the laminating of the inside bottom surface that bears groove ring (8), control valve (29) are all run through to install to the up end of intake pipe (4) and the up end of blast pipe (3), stir recess (34) have been run through to the side of bearing groove ring (8).
6. The microorganism culture apparatus according to claim 5, wherein: the bearing groove ring (8) and the exhaust pipe (3) are integrally formed, four groups of bearing frame bodies (32) extend from the annular array of the outer surface of the bearing groove ring (8), limiting sliding grooves (30) are formed in the upper end face and the lower end face of the bearing frame bodies (32) in a penetrating mode, a pressing block (28) is mounted in the bearing frame bodies (32) in a fitting mode, four groups of through holes (35) are formed in the annular array of the outer surface of the bearing groove ring (8) in a penetrating mode, the bearing frame bodies (32) are located on the side of the through holes (35), the pressing block (28) is attached to the inside of the through holes (35), the edge of one side outer surface of the pressing block (28) is provided with an inclined plane, the other side outer surface of the pressing block (28) is pressed on the side surface of the filter screen (27), the upper end surface and the lower end surface of the pressing block (28) are respectively extended with a limiting slide block (31), the pressing block (28) and the limiting slide blocks (31) are integrally formed, the limiting sliding block (31) is attached to the inside of the limiting sliding groove (30), a spring body (33) extends from the front end face of the inside of the bearing frame body (32), and the end portion of the spring body (33) is fixedly connected with the rear end face of the pressing block (28).
7. A method of culturing a microbial culture apparatus according to claim 5 or 6 comprising the steps of: s1: placing a tray (24) containing food on the support, then injecting the culture solution through a liquid inlet pipe (7), and sprinkling the culture solution on the food through a liquid adding tank tray (19);
s2: then the interior of the shell (1) is heated by the heating lamp (10), and meanwhile, the driving piece drives the heating lamp (10) to rotate and heat, so that the temperature in the shell (1) reaches the culture temperature for culture;
s3: and open blast pipe (3) and intake pipe (4) regularly, the waste gas that produces when making cultivateing in casing (1) can discharge, and oxygen gets into in casing (1) through intake pipe (4) simultaneously to guarantee good air quality in casing (1), make cultivate can normally go on.
CN202110938600.2A 2021-08-16 2021-08-16 Microorganism culture device and culture method thereof Active CN113699022B (en)

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CN112662522A (en) * 2021-01-22 2021-04-16 魏乾雪 A atomizing device for cell culture liquid
CN112877183A (en) * 2021-01-29 2021-06-01 武汉兰卫医学检验实验室有限公司 Medical science inspection microbiological culture apparatus
CN213506885U (en) * 2020-10-21 2021-06-22 云南微态源生物科技有限公司 Microbial cultivation device of batch addition culture solution
CN213835263U (en) * 2020-08-28 2021-07-30 上海昌进生物科技有限公司 Microbial cultivation case with categorised function of cultivateing
CN213951183U (en) * 2020-09-24 2021-08-13 王瑜婷 Liquid separating device for production of microbial culture medium

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JP2010136666A (en) * 2008-12-11 2010-06-24 Panasonic Corp Automatically culturing apparatus and automatically culturing method
DE202014103237U1 (en) * 2014-07-14 2014-07-25 Taiwan Leader Biotech Corp. Fully functional solid fermentation device
CN209162055U (en) * 2018-11-02 2019-07-26 曲靖医学高等专科学校 A kind of microbe research culture apparatus
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