CN113699022A

CN113699022A - Microbial culture medium and culture method thereof

Info

Publication number: CN113699022A
Application number: CN202110938600.2A
Authority: CN
Inventors: 王寒微
Original assignee: Tianjin Hongjincheng Biotechnology Co ltd
Current assignee: Shanghai Zhichu Instrument Co ltd
Priority date: 2021-08-16
Filing date: 2021-08-16
Publication date: 2021-11-26
Anticipated expiration: 2041-08-16
Also published as: CN113699022B

Abstract

The invention relates to the field of microorganism culture, in particular to a microorganism culture medium which comprises a shell, a driving piece is arranged on the lower end surface of the machine shell in a penetrating way, a bearing rod is arranged on the outer side of the driving piece, the side surface of the bearing rod is fixedly connected with a heating lamp, a guide piece is arranged between the inner part of the machine shell and the bearing rod, a liquid inlet pipe is inserted in the middle of the upper end surface of the casing in a penetrating and rotating way, a plurality of groups of liquid distributing pipes are fixedly communicated with the outer surface of the liquid inlet pipe in an annular array, the end part of the liquid distribution pipe is fixedly communicated with a nozzle, the outer side of the nozzle is provided with a blocking cover shell, the invention also provides a culture method of the microorganism culture medium, which can improve the culture effect, and the phenomenon that the food is damaged due to overhigh pressure of the sprayed culture medium can be avoided, and the use convenience is improved.

Description

Microbial culture medium and culture method thereof

Technical Field

The invention relates to the field of microbial culture, in particular to a microbial culture medium and a culture method thereof.

Background

Microorganisms are a generic term for all microorganisms that are difficult for an individual to observe with the naked eye. The microorganism includes bacterium, virus, fungi and a few algae etc. through magnifying the microscope of 50 ~ 300 times, can see bacterium and protozoan clearly, among the prior art when cultivateing the microorganism in the food through the microbial cultivation medium, because of the culture solution pressure through the nozzle spun is too big, cause the culture solution easily to cause the damage to food when spouting on food, and when controlling the temperature of cultivateing through the heat lamp, because of the heat lamp is fixed state, cause the food adjacent with the heat lamp to cause the food overheat because of being heated for a long time to influence the phenomenon that the microorganism was cultivateed.

Disclosure of Invention

The invention aims to solve the defects in the background art and provides a microbial culture medium and a culture method thereof.

In order to achieve the above purposes, the technical scheme adopted by the invention is as follows: the utility model provides a microbial cultivation medium, includes the casing, the lower terminal surface of casing runs through and installs the driving piece, the carrier bar is installed in the outside of driving piece, the side fixedly connected with heat lamp of carrier bar, install the guiding piece between the inside of casing and the carrier bar, the up end middle part of casing runs through to rotate and inlays and have the feed liquor pipe, the fixed intercommunication of surface annular array of feed liquor pipe has the multiunit to divide liquid pipe, the end fixing intercommunication that divides liquid pipe has the nozzle, the outside of nozzle is provided with blocks the housing, the lower terminal surface that blocks the housing is inlayed and is had the liquid feeding groove dish, the support article is installed in the outside of feed liquor pipe, the support article is located the below of liquid feeding groove dish, the side of casing is run through and is installed the air discharge spare.

Preferably, the inside top surface fixed mounting of casing has the temperature controller, the lower terminal surface of casing extends there is the shell seat, the preceding terminal surface of casing rotates and installs sealing door, the preceding terminal surface of sealing door runs through to inlay has clear glass, the driving piece is including running through the drive shaft of inlaying at the lower terminal surface middle part of casing, the coaxial fixed mounting in lower tip of drive shaft has servo just reversing motor, servo just reversing motor is connected with the shell seat, the drive shaft is connected with the carrier bar.

Preferably, the side of casing is inlayed and is had the motor frame, the tip of motor frame and the side fixed connection of servo positive reverse rotation motor, the surface annular array of drive shaft extends and has three couples of framves, the surface annular array slope of drive shaft extends and has three couples of enhancement framves, the enhancement frame is located the link under, the tip of link and the tip of enhancement frame all with the surface fixed connection of carrier bar, the coaxial carousel of inlaying in the outside of feed liquor pipe, the carousel is located the top of casing.

Preferably, the guide part comprises a guide ring embedded in the inner outer surface of the casing, a connecting wheel shaft extends from the front end surface of the bearing rod, a guide wheel is rotatably mounted on the outer side of the connecting wheel shaft, and the guide wheel is attached to the outer surface of the guide ring.

Preferably, the inside surface of liquid feeding frid inlays and has linking frame, the tip fixed mounting that links up the frame has the dispersion post, the up end of dispersion post is opened and is established to the conical surface, the axial lead of dispersion post and the axial lead of nozzle are located same plumb line, liquid feeding frid and the shell integrated into one piece that blocks, it has the spliced pole to block the inside surface top edge department extension of shell, the tip of spliced pole and the fixed surface of nozzle are connected.

Preferably, the support object comprises a bearing support ring fixedly installed on the outer side of the liquid inlet pipe, a plurality of groups of support discs extend from the upper end face of the bearing support ring in an annular array mode, the support discs are located under the liquid adding groove disc, and a plurality of groups of round holes are formed in the bottom face of the inner portion of the liquid adding groove disc in a penetrating mode.

Preferably, the air discharge part is including running through the intake pipe of inlaying on one side upper portion of casing, the opposite side upper portion of casing runs through to inlay has the blast pipe, the coaxial extension of tip of blast pipe has the bearing groove ring, the cross sectional shape of bearing groove ring is L shape, the filter screen is installed in the laminating of the inside bottom surface of bearing groove ring, the up end of intake pipe all runs through with the up end of blast pipe and installs the control valve, the side of bearing groove ring runs through to have seted up stirs the recess.

Preferably, the bearing groove ring and the exhaust pipe are integrally formed, four groups of bearing frame bodies extend from the outer surface of the bearing groove ring in an annular array, the upper end surface and the lower end surface of the bearing frame body are both provided with limiting chutes in a penetrating way, the inside of the bearing frame body is provided with a pressing block in a fitting way, the outer surface of the bearing groove ring is provided with four groups of through holes in a penetrating way in an annular array way, the bearing frame body is positioned on the side of the through holes, the pressing block is attached to the inside of the through hole, the edge of the outer surface of one side of the pressing block is provided with an inclined plane, the outer surface of the other side of the pressing block is tightly pressed on the side surface of the filter screen, the upper end surface and the lower end surface of the pressing block extend to form limiting sliding blocks, the pressing block and the limiting slide block are integrally formed, the limiting slide block is attached to the inside of the limiting slide groove, the spring body extends from the front end face of the interior of the bearing frame body, and the end part of the spring body is fixedly connected with the rear end face of the pressing block.

Also provided is a method for culturing a microbial culture medium, comprising the following steps:

s1: placing the tray filled with the food on a support object, injecting the culture solution through a liquid inlet pipe, and sprinkling the culture solution on the food through a liquid adding trough plate;

s2: heating the interior of the machine shell by using a heating lamp, and simultaneously driving the heating lamp to rotate and heat by using a driving piece so as to enable the temperature in the machine shell to reach a culture temperature for culture;

s3: and regularly open blast pipe and intake pipe, the waste gas that produces when making the casing in cultivate can discharge, and oxygen passes through the intake pipe simultaneously and gets into in the casing to guarantee good air quality in the casing, make and cultivate and can normally go on.

Compared with the prior art, the invention has the following beneficial effects:

1. can drive the carrier bar and rotate under the drive of pivoted drive shaft, guide wheel on the carrier bar rolls on the guide ring at this moment rotates around the axle center of guide ring with supplementary carrier bar, and then drive the heat lamp on the carrier bar and rotate the heating round the guide ring, when avoiding heating in to the casing through the heat lamp, food adjacent with the heat lamp can be because of being heated for a long time and cause the overheated phenomenon that influences the microorganism culture of food, so that improve its cultivation effect, simultaneously under the effect of annular array's multicomponent liquid pipe, can divide the interior culture solution of feed liquor pipe and derive and fall on the tray under the effect of the multiunit round hole of feeding liquid groove dish bottom, so as to guarantee that food on the tray can be abundant cultivate with the contact of culture solution, in order to further improve its cultivation effect.

2. The culture solution sprayed through the nozzle can directly impact on the dispersion column to disperse and impact the sprayed culture solution on the inner walls of the blocking housing and the liquid adding groove plate through the dispersion column, so that the culture solution can fall along the inner walls of the blocking housing and the liquid adding groove plate and fall down on food through the discharge of the round holes at the bottom of the liquid adding groove plate, and the phenomenon that the food is damaged due to too high pressure of the culture solution sprayed from the nozzle is avoided.

3. Restriction slider can be ordered about through directly stirring and restrict the slider and slide in the restriction spout to drive and to be promoted fixed the cover piece removal by the spring body and break away from in the through-hole, make the filter screen that is pressed the cover piece and compress tightly can move about, stimulate the filter screen afterwards, in order to take out the filter screen from the bearing groove intra-annular and to maintain the change, with this effectual subsequent maintenance change that has made things convenient for, improved convenient to use degree.

Drawings

FIG. 1 is a schematic structural view of a microorganism culture medium according to the present invention;

FIG. 2 is an internal view of a housing for a microbiological culture medium according to the invention;

FIG. 3 is a partial view of the interior of a housing for a microbiological culture medium according to the invention;

FIG. 4 is a cross-sectional view of a microbiological culture media barrier enclosure in accordance with the present invention;

FIG. 5 is a schematic view of a microbial growth medium of the present invention at the exhaust pipe;

FIG. 6 is a schematic view of a microorganism culture medium according to the present invention at a laminated block.

In the figure: 1. a housing; 2. a sealing door; 3. an exhaust pipe; 4. an air inlet pipe; 5. a housing base; 6. rotating the disc; 7. a liquid inlet pipe; 8. a bearing groove ring; 9. a guide ring; 10. a heating lamp; 11. a blocking enclosure; 12. a servo positive and negative rotation motor; 13. a motor frame; 14. a connecting frame; 15. a reinforcing frame; 16. a drive shaft; 17. a liquid separating pipe; 18. a nozzle; 19. a liquid feeding trough plate; 20. a carrier bar; 21. connecting the wheel shaft; 22. a guide wheel; 23. a bearing support ring; 24. a tray; 25. a dispersion column; 26. a linking frame; 27. filtering with a screen; 28. pressing the covering block; 29. a control valve; 30. a restricting chute; 31. a limiting slide block; 32. a bearing frame body; 33. a spring body; 34. poking the groove; 35. and a through hole.

Detailed Description

The following description is presented to disclose the invention so as to enable any person skilled in the art to practice the invention. The preferred embodiments in the following description are given by way of example only, and other obvious variations will occur to those skilled in the art.

A microorganism culture medium as shown in FIGS. 1-6 comprises a casing 1, a driving member is installed on the lower end surface of the casing 1 in a penetrating manner, a bearing rod 20 is installed on the outer side of the driving member, a heating lamp 10 is fixedly connected to the side surface of the bearing rod 20, a guiding member is installed between the inner part of the casing 1 and the bearing rod 20, a liquid inlet pipe 7 is rotatably inserted in the middle of the upper end surface of the casing 1, a multi-component liquid pipe 17 is fixedly communicated with the outer surface of the liquid inlet pipe 7 in an annular array manner, a nozzle 18 is fixedly communicated with the end part of the liquid distribution pipe 17, a blocking housing 11 is arranged on the outer side of the nozzle 18, a liquid feeding tray 19 is inserted on the lower end surface of the blocking housing 11, a holding member is installed on the outer side of the liquid inlet pipe 7 and is located below the liquid feeding tray 19, an air exhausting member is installed on the side surface of the casing 1 in a penetrating manner, the culture liquid distribution pipe 7 can be separately guided into the nozzle 18 and sprayed out through the liquid distribution pipe 17, the heating lamp 10 plays a role of heating, and the temperature controller plays a role of controlling temperature, so that the heating lamp 10 is controlled to stop working after the temperature is reached.

The inside top surface fixed mounting of casing 1 has the temperature controller, the lower terminal surface of casing 1 extends there is housing 5, the preceding terminal surface of casing 1 rotates and installs sealing door 2, the preceding terminal surface of sealing door 2 runs through to inlay has clear glass, the driving piece is including running through to rotate and inlaying the drive shaft 16 at the lower terminal surface middle part of casing 1, the coaxial fixed mounting of lower tip of drive shaft 16 has servo positive and negative motor 12 that changes, servo positive and negative motor 12 that changes is connected with housing 5, drive shaft 16 is connected with carrier bar 20, can support casing 1 through the housing 5 that sets up, sealing door 2 plays the effect of sealing up casing 1, servo positive and negative motor 12 can drive shaft 16 and change forward and reverse, drive heating lamp 10 on carrier bar 20 and carry out the antiport 120 degrees after rotating 120 degrees promptly, with this circulation.

The motor frame 13 is inlaid in the side face of the shell base 5, the end portion of the motor frame 13 is fixedly connected with the side face of the servo forward and reverse rotation motor 12, three groups of connecting frames 14 extend from the outer surface annular array of the driving shaft 16, three groups of reinforcing frames 15 extend from the outer surface annular array of the driving shaft 16 in an inclined mode, the reinforcing frames 15 are located under the connecting frames 14, the end portion of each connecting frame 14 and the end portion of each reinforcing frame 15 are fixedly connected with the outer surface of the bearing rod 20, the rotating disc 6 is inlaid in the outer side of the liquid inlet pipe 7 in a coaxial mode, the rotating disc 6 is located above the shell 1, the bearing rod 20 can be connected with the driving shaft 16 through the arranged connecting frames 14, the reinforcing frames 15 can reinforce the joint of the bearing rod 20 and the driving shaft 16, and the motor frame 13 plays a role in fixing the servo forward and reverse rotation motor 12.

The guide is including inlaying at the inside surface's of casing 1 guide ring 9, the preceding terminal surface of carrier bar 20 extends there is connecting wheel axle 21, connecting wheel axle 21's outside is rotated and is installed guide wheel 22, guide wheel 22 laminates in guide ring 9's surface, can prop the support to guide wheel 22 through the guide ring 9 that sets up, connecting wheel axle 21 plays the effect of bearing guide wheel 22, guide wheel 22 plays the guide, the effect of propping the support, with the bearing capacity that reduces carrier bar 20.

The inner outer surface of the liquid adding groove disc 19 is inlaid with a connecting frame 26, the end part of the connecting frame 26 is fixedly provided with a dispersing column 25, the upper end surface of the dispersing column 25 is provided with a conical surface, the axial lead of the dispersing column 25 and the axial lead of the nozzle 18 are positioned in the same vertical line, the liquid adding groove disc 19 and the blocking housing 11 are integrally formed, the upper edge part of the inner outer surface of the blocking housing 11 is extended with a connecting column, the end part of the connecting column is fixedly connected with the outer surface of the nozzle 18, the dispersing column 25 can be fixed through the arranged connecting frame 26, the dispersing column 25 with the conical surface arranged on the upper end surface can disperse the culture solution, the blocking housing 11 plays a role in blocking the dispersed culture solution, and the connecting column plays a role in connecting the blocking housing 11 and the nozzle 18 together.

The tray article includes the bearing ring 23 of fixed mounting in the outside of feed liquor pipe 7, the annular array of up end that bears ring 23 extends has multiunit tray 24, tray 24 is located liquid feeding tray 19 under, liquid feeding tray 19's inside bottom surface runs through and has seted up multiunit round hole, can fix tray 24 through the bearing ring 23 that sets up, tray 24 can be placed the tray that is equipped with food, liquid feeding tray 19's inside bottom surface runs through and has seted up multiunit round hole, can make the even dropping on food of culture solution in the liquid feeding tray 19.

The exhaust part comprises an air inlet pipe 4 which penetrates through and is embedded at the upper part of one side of the machine shell 1, an exhaust pipe 3 penetrates and is embedded at the upper part of the other side of the machine shell 1, a bearing groove ring 8 coaxially extends at the end part of the exhaust pipe 3, the cross section of the bearing groove ring 8 is L-shaped, a filter screen 27 is attached and installed at the inner bottom surface of the bearing groove ring 8, a control valve 29 is respectively installed at the upper end surface of the air inlet pipe 4 and the upper end surface of the exhaust pipe 3 in a penetrating way, a toggle groove 34 is arranged at the side surface of the bearing groove ring 8 in a penetrating way, can be with the leading-in casing 1 of oxygen in through the intake pipe 4 that sets up, can keep the abundant state of oxygen in the casing 1 when making the cultivation, the exhaust-gas discharge that produces when blast pipe 3 can be cultivateed, filter screen 27 plays the effect of purifying waste gas, is made by activated carbon, and control valve 29 plays the effect of opening and close intake pipe 4 and blast pipe 3, stirs recess 34 and can be convenient for pull out filter screen 27 and take out.

The bearing groove ring 8 and the exhaust pipe 3 are integrally formed, four groups of bearing frame bodies 32 extend from the outer surface annular array of the bearing groove ring 8, the upper end surface and the lower end surface of each bearing frame body 32 are respectively provided with a limiting sliding groove 30 in a penetrating way, a pressing block 28 is installed inside each bearing frame body 32 in a fitting way, four groups of through holes 35 extend from the outer surface annular array of the bearing groove ring 8, each bearing frame body 32 is positioned at the side of each through hole 35, each pressing block 28 is fitted inside each through hole 35, the edge of the outer surface at one side of each pressing block 28 is provided with an inclined surface, the outer surface at the other side of each pressing block 28 is pressed on the side surface of the filter screen 27, limiting sliding blocks 31 extend from the upper end surface and the lower end surface of each pressing block 28, each pressing block 28 and each limiting sliding block 31 are integrally formed, each limiting sliding block 31 is fitted inside each limiting sliding groove 30, a spring body 33 extends from the front end surface inside each bearing frame body 32, and the end part of each spring body 33 is fixedly connected with the rear end surface of each pressing block 28, the bearing frame body 32 can limit the pressing block 28, the pressing block 28 can fix the filter screen 27, the limiting sliding block 31 and the limiting sliding groove 30 can guarantee that the pressing block 28 moves linearly, the spring body 33 plays a role in pushing the pressing block 28, and the pressing block 28 is fixed on the filter screen 27.

s1: placing the tray filled with the food on a tray object, then injecting the culture solution through the liquid inlet pipe 7, and sprinkling the culture solution on the food through the liquid adding trough plate 19;

s2: heating the interior of the machine shell 1 by the heating lamp 10, and simultaneously driving the heating lamp 10 to rotate and heat by the driving piece so as to enable the temperature in the machine shell 1 to reach the culture temperature for culture;

s3: and regularly open blast pipe 3 and intake pipe 4, the waste gas that produces when making the interior cultivation of casing 1 can be discharged, and oxygen passes through intake pipe 4 simultaneously and gets into in the casing 1 to guarantee the interior good air quality of casing 1, make the cultivation can normally go on.

During operation, the tray containing food is placed in the tray 24, then the culture solution is injected through the liquid inlet pipe 7, the culture solution enters the nozzle 18 under the guide of the liquid separating pipe 17 and is sprayed out, the culture solution sprayed out through the nozzle 18 directly impacts the dispersion column 25, the sprayed culture solution is dispersed and impacts on the inner walls of the blocking housing 11 and the liquid adding trough plate 19 through the dispersion column 25, the culture solution can fall along the inner walls of the blocking housing 11 and the liquid adding trough plate 19 and is discharged onto the food through the round hole at the bottom of the liquid adding trough plate 19, the heating lamp 10 works and heats at the same time, the temperature in the machine shell 1 suitable for cultivation is achieved, the servo forward and reverse rotation motor 12 works to drive the driving shaft 16 to rotate, the bearing rod 20 connected to the driving shaft 16 through the connecting frame 14 and the reinforcing frame 15 is driven to rotate, the guide wheel 22 on the bearing rod 20 rolls on the guide ring 9 to assist the bearing rod 20 to rotate around the axis of the guide ring 9 Rotate, and then drive the heat lamp 10 on the carrier bar 20 and rotate the heating round guide ring 9 to this cultivates, when cultivateing, can regularly open blast pipe 3 and intake pipe 4, and the waste gas that produces when making the interior cultivation of casing 1 can be discharged, and oxygen passes through intake pipe 4 simultaneously and gets into in the casing 1, in order to guarantee good air quality in the casing 1, makes the cultivation can normally go on.

In summary, the carrier bar 20 can be driven to rotate by the rotating driving shaft 16, and the guiding wheel 22 on the carrier bar 20 rolls on the guiding ring 9 to assist the carrier bar 20 to rotate around the axis of the guiding ring 9, thereby driving the heating lamps 10 on the carrying rod 20 to rotate around the guiding ring 9 for heating, so as to avoid that when the interior of the cabinet 1 is heated by the heating lamps 10, the food adjacent to the heating lamp 10 is heated for a long time to cause the phenomenon that the food is overheated to affect the microbial cultivation, thereby improving the cultivation effect, meanwhile, under the action of the multi-component liquid tubes 17 in the annular array, the culture solution in the liquid inlet tube 7 can be separately led out and fall on the tray 24 under the action of a plurality of groups of round holes at the bottom of the liquid adding trough tray 19, therefore, the food on the tray 24 can be fully contacted with the culture solution for culture, so that the culture effect is further improved; the culture solution sprayed out through the nozzle 18 directly impacts on the dispersion column 25, so that the sprayed culture solution is dispersed and impacted on the inner walls of the blocking housing 11 and the liquid adding trough plate 19 through the dispersion column 25, the culture solution can fall along the inner walls of the blocking housing 11 and the liquid adding trough plate 19 and is discharged onto the falling food through the round hole at the bottom of the liquid adding trough plate 19, and the phenomenon that the food is damaged due to overhigh culture solution pressure sprayed out from the nozzle 18 is avoided; restriction slider 31 is shifted through directly, can order about restriction slider 31 and slide in restriction spout 30 to drive and to be promoted fixed the removal of pressing the cover piece 28 by spring body 33 and break away from in the through-hole 35, make the filter screen 27 that is pressed the cover piece 28 and compresses tightly can move about, stimulate filter screen 27 afterwards, take out filter screen 27 from bearing the weight of the slotted ring 8 and can maintain the change, with this effectual subsequent maintenance change of having made things convenient for, improved convenient to use degree.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A microbiological culture medium comprising a housing (1), characterized in that: a driving piece penetrates through the lower end surface of the machine shell (1), a bearing rod (20) is arranged on the outer side of the driving piece, the side surface of the bearing rod (20) is fixedly connected with a heating lamp (10), a guide piece is arranged between the interior of the machine shell (1) and the bearing rod (20), a liquid inlet pipe (7) is inserted in the middle of the upper end surface of the casing (1) in a penetrating and rotating way, the outer surface of the liquid inlet pipe (7) is fixedly communicated with a plurality of groups of liquid distributing pipes (17) in an annular array, the end part of the liquid separating pipe (17) is fixedly communicated with a nozzle (18), a blocking cover shell (11) is arranged outside the nozzle (18), a liquid feeding groove disc (19) is embedded on the lower end surface of the blocking encloser (11), a supporting object is arranged on the outer side of the liquid inlet pipe (7), the object supporting piece is positioned below the liquid adding trough plate (19), and an exhaust piece penetrates through the side surface of the machine shell (1).

2. A microbiological culture medium according to claim 1 wherein: the inside top surface fixed mounting of casing (1) has the temperature controller, the lower terminal surface of casing (1) extends there is shell seat (5), the preceding terminal surface of casing (1) rotates and installs sealing door (2), the preceding terminal surface of sealing door (2) runs through to inlay and has clear glass, the driving piece is including running through drive shaft (16) of rotating and inlaying at the lower terminal surface middle part of casing (1), the coaxial fixed mounting in lower tip of drive shaft (16) has servo just reversing motor (12), servo just reversing motor (12) are connected with shell seat (5), drive shaft (16) are connected with carrier bar (20).

3. A microbiological culture medium according to claim 2 wherein: the side of housing (5) is inlayed and is had motor frame (13), the side fixed connection of the tip of motor frame (13) and servo positive reverse rotation motor (12), the surface annular array of drive shaft (16) extends and has three group link (14), the surface annular array slope of drive shaft (16) extends and has three group enhancement frame (15), it is located link (14) under enhancement frame (15), the tip of link (14) and the tip of enhancement frame (15) all with the surface fixed connection of carrier bar (20), the coaxial inlaying in the outside of feed liquor pipe (7) has rolling disc (6), rolling disc (6) are located the top of casing (1).

4. A microbiological culture medium according to claim 1 wherein: the guide piece is including inlaying at the inside surface's of casing (1) guide ring (9), the preceding terminal surface of carrier bar (20) extends and has connect shaft (21), connect the outside of shaft (21) and rotate and install guide wheel (22), guide wheel (22) laminate in the surface of guide ring (9).

5. A microbiological culture medium according to claim 1 wherein: the inside surface of liquid feeding frid (19) inlays and has linking frame (26), the tip fixed mounting who links up frame (26) has dispersion post (25), the up end of dispersion post (25) is opened and is established to the conical surface, the axial lead of dispersion post (25) and the axial lead of nozzle (18) are located same perpendicular line, liquid feeding frid (19) with block housing (11) integrated into one piece, the inside surface top edge department that blocks housing (11) extends has the spliced pole, the tip of spliced pole and the surface fixed connection of nozzle (18).

6. A microbiological culture medium according to claim 1 wherein: the tray comprises a bearing supporting ring (23) fixedly mounted on the outer side of the liquid inlet pipe (7), a plurality of groups of tray discs (24) extend from the upper end face of the bearing supporting ring (23) in an annular array mode, the tray discs (24) are located under the liquid adding groove disc (19), and a plurality of groups of round holes are formed in the bottom face of the inner portion of the liquid adding groove disc (19) in a penetrating mode.

7. A microbiological culture medium according to claim 1 wherein: the air exhaust part is including running through intake pipe (4) of inlaying on one side upper portion of casing (1), the opposite side upper portion of casing (1) runs through to inlay has blast pipe (3), the coaxial extension of tip of blast pipe (3) has bearing groove ring (8), the cross sectional shape who bears groove ring (8) is L shape, bearing groove ring (8) inside bottom surface laminating installs filter screen (27), control valve (29) are all run through to install with the up end of blast pipe (3) to the up end of intake pipe (4), bearing groove ring (8) side runs through to have seted up and stirs recess (34).

8. A microbiological culture medium according to claim 7 wherein: the bearing groove ring (8) and the exhaust pipe (3) are integrally formed, the outer surface annular array of the bearing groove ring (8) extends to form four groups of bearing frame bodies (32), the upper end face and the lower end face of each bearing frame body (32) are all penetrated and provided with limiting sliding grooves (30), the bearing frame bodies (32) are internally laminated and provided with pressing covering blocks (28), the outer surface annular array of the bearing groove ring (8) is penetrated and provided with four groups of through holes (35), the bearing frame bodies (32) are positioned on the side faces of the through holes (35), the pressing covering blocks (28) are laminated in the through holes (35), the edge of the outer surface on one side of each pressing covering block (28) is provided with an inclined plane, the outer surface on the other side of each pressing covering block (28) is pressed on the side face of the filter screen (27), the limiting sliding blocks (31) are respectively extended from the upper end face and the lower end face of each pressing covering block (28), and the pressing covering blocks (28) and the limiting sliding blocks (31) are integrally formed, the limiting sliding block (31) is attached to the inside of the limiting sliding groove (30), a spring body (33) extends from the front end face of the inside of the bearing frame body (32), and the end portion of the spring body (33) is fixedly connected with the rear end face of the pressing and covering block (28).

9. A method of culturing a microbial culture medium according to any one of claims 1 to 8, comprising the steps of: s1: placing the tray filled with the food on a tray object, then injecting the culture solution through the liquid inlet pipe (7) and sprinkling the culture solution on the food through the liquid adding groove tray (19);

s2: then, the interior of the machine shell (1) is heated through the heating lamp (10), and meanwhile, the driving piece drives the heating lamp (10) to rotate and heat, so that the temperature in the machine shell (1) reaches the culture temperature for culture;

s3: and regularly open blast pipe (3) and intake pipe (4), the waste gas that produces when making in casing (1) cultivate can be discharged, and oxygen passes through intake pipe (4) and gets into in casing (1) simultaneously to guarantee the interior good air quality of casing (1), make and cultivate and can normally go on.