CN1880443A - Portable culture device for liquid strain of edible fungus and using method therefor - Google Patents
Portable culture device for liquid strain of edible fungus and using method therefor Download PDFInfo
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- CN1880443A CN1880443A CN 200610081643 CN200610081643A CN1880443A CN 1880443 A CN1880443 A CN 1880443A CN 200610081643 CN200610081643 CN 200610081643 CN 200610081643 A CN200610081643 A CN 200610081643A CN 1880443 A CN1880443 A CN 1880443A
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- pipe
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- bacterial classification
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Abstract
The invention discloses a portable edible mushroom liquid bacterial culturing device and utility method in the edible mushroom culturing and applying domain, which comprises the following steps: setting rubber lid on the transparent culture bottle with pipe group of switch-in and fetching bacterial pipe and oxygenation and venting pipe in the middle; setting sealing lid on the upper end of pipe; setting two outlets on the pipe tee (oxygenation and venting pipe); extending one outlet pipe in the bottle bottom in connection with a bubble stone; assembling filter cotton in the middle of upper pipe of pipe tee with upper lid on the pipe with projected pipe.
Description
Technical field
The present invention relates to the cultivation and methods for using them of edible fungus species, specifically portable culture device for liquid strain of edible fungus and using method.
Background technology
Traditional edible fungi culture method is after adopting solid vaccine enforcement inoculation culture to go out bacterial classification, the cultivation bag to be inoculated again, thereby reached the method that cultivates edible mushrooms.Adopt traditional breeding and employed cycle of cultivating method longer, the cost height is taken a lot of work again, and the assorted bacterium of easy infection.For this reason, people have carried out a large amount of discussion research to the edible fungi liquid strain seeding technique over past ten years, and have obtained certain effect.The successful Application of liquid spawn makes the whole Edible Fungi cycle, has obtained shortening widely, and has saved a lot of labours, is subjected to the favor of vast mushroom farming deeply.Yet though liquid spawn has its advantage, the making of having released at present cultivates liquid spawn equipment manufacturing cost height, when making difficulty big, have a big risk, culture success ratio is not high, therefore, brought up the hysteresis of popularizing.
Show that from the pertinent data retrieval that comprises Chinese patent 116 of existing at present Chinese patent application or disclosed pertinent literatures are as CN01803101.3＜liquid bacterial vaccinating device 〉; CN01114001.1＜edible fungi liquid strain production method and equipment 〉; CN02268780.7＜a kind of bacterial classification liquid production unit 〉; CN89203999.X＜simple and easy liquid spawn production equipment 〉; 200510034376.5＜portable liquid-spawn inoculation bottle 〉; 200420078675.X＜strain cultivation device 〉; CN200520008096.2＜efficient liquid seed bottle device etc.Mainly have the spawn culture process for various reasons from the prior art analysis, often cause problems such as success ratio is low, therefore the production cycle in whole season has been missed in many places, so use existing liquid spawn equipment in culturing process, feels not only complexity but also impracticable.In addition, there are some to install the not only big but also stupid medium and small large-scale production that is not easy in the report.Therefore, solve portable and problem culture success ratio is the task of top priority.
Summary of the invention
In order to overcome the problem that has the low and equipment complexity of surviving rate etc. in the prior art, the objective of the invention is to provide a kind of portable culture device for liquid strain of edible fungus and using method with oxygenation function.
The technical solution adopted for the present invention to solve the technical problems is: a kind of portable culture device for liquid strain of edible fungus with oxygenation function, it is characterized in that: the culturing bottle that has a transparent material to make, a serum cap is installed on the bottleneck, and the centre of serum cap is inserted with by connecing, get the pipeline group that bacterial classification pipeline and oxygenation and ventpipe pipe are formed; The described upper port place that connects, gets the bacterial classification pipeline is provided with sealing cover; Described oxygenation and breather line are Y-tube, its lower end is provided with two outlets, at the bottom of one of them outlet conduit of pipeline extend into bottle, is connected with a Bubbled stone on the pipeline opening at the bottom of stretching into bottle, in the middle of the upper pipes of Y-tube filter cotton is installed, is stamped the loam cake that has the tang pipe on its pipeline opening.Be carved with metering scale on the described transparent culturing bottle.
Production method of the present invention is characterized in that:
1, gets the culturing bottle that cleans up and nutrient solution packed into;
2, add a cover and insert pipeline;
3, culturing bottle is carried out high-temperature sterilization with ordinary method;
4, open the sealing cover that connects, gets the bacterial classification mouth of pipe, connect spawn culture 24～72 hours, covered loam cake, and carried out 5～30 minutes oxygenation in wherein per 6～10 hours;
5, after cultivation is finished culturing bottle is put into box bucket, open and connect, get bacterial classification seal for pipe joints mouth, insert the bacterial classification rifle;
6, to cultivation bag injecting fluid bacterial classification.
The invention has the beneficial effects as follows: because the present invention is designed to culturing bottle to make of transparent material, be convenient to like this state of nutrient solution is carried out visual inspection, secondly the present invention is designed to oxygenation and air inlet dual-purpose pipe with breather line, when needs oxygenation, in time nutrient solution is carried out oxygenation like this, thereby guaranteed that liquid spawn grows up under aerobic state, improved its surviving rate widely, guaranteed to produce needs with bacterial classification.Therefore, the present invention can reach the purpose of drafting.
Description of drawings
Below in conjunction with drawings and Examples the present invention is further described.
Fig. 1 is the synoptic diagram of structure of the present invention.
Fig. 2 is the view that the present invention analyses and observe back pipe group.
1 culturing bottle among the figure, 2 serum caps, 3 connect, get the bacterial classification pipeline; 4 oxygenation and breather line, 5 sealing covers, 6 Bubbled stones, 7 filter cottons, 8 loam cakes.
Embodiment
Embodiment 1:
See also Fig. 1 and Fig. 2, what have that transparent material makes is carved with 3000 liters culturing bottle 1, and a serum cap 2 is installed on the bottleneck, and the centre of serum cap 2 is inserted with by connecing, get the pipeline group that bacterial classification pipeline 3 and oxygenation and breather line 4 pipes are formed; The described upper port place that connects, gets bacterial classification pipeline 3 is provided with sealing cover 5; Described oxygenation and breather line 4 are Y-tube, its lower end is provided with two outlets, at the bottom of one of them outlet conduit of pipeline extend into bottle, be connected with a Bubbled stone 6 on the pipeline opening at the bottom of stretching into bottle, in the middle of the upper pipes of Y-tube filter cotton 7 is installed, is stamped the loam cake 8 that has the tang pipe on its pipeline opening.
The production method of present embodiment:
1, gets the culturing bottle that cleans up and 3000 liters of nutrient solutions are packed into;
2, add a cover and insert pipeline;
3, culturing bottle is carried out high-temperature sterilization with ordinary method;
4, open the sealing cover that connects, gets the bacterial classification mouth of pipe, connect mushroom strain and cultivated 50 hours, covered loam cake, and carried out 10 minutes oxygenation in wherein per 10 hours;
5, after cultivation is finished culturing bottle is put into box bucket, open and connect, get bacterial classification seal for pipe joints mouth, insert the bacterial classification rifle.
6, to cultivation bag injecting fluid bacterial classification.
Claims (5)
1. portable culture device for liquid strain of edible fungus with oxygenation function, it is characterized in that: a culturing bottle is arranged, serum cap is installed on the bottleneck, the centre of serum cap is inserted with by connecing, get the pipeline group that bacterial classification pipeline and oxygenation and ventpipe pipe are formed; The described upper port place that connects, gets the bacterial classification pipeline is provided with sealing cover; Described oxygenation and breather line are Y-tube, its lower end is provided with two outlets, at the bottom of one of them outlet conduit of pipeline extend into bottle, is connected with a Bubbled stone on the pipeline opening at the bottom of stretching into bottle, in the middle of the upper pipes of Y-tube filter cotton is installed, is stamped loam cake on its pipeline opening.
2. portable culture device for liquid strain of edible fungus according to claim 1 is characterized in that: described culturing bottle is that transparent material is made.
3. portable culture device for liquid strain of edible fungus according to claim 1 is characterized in that: be carved with metering scale on the described culturing bottle.
4. portable culture device for liquid strain of edible fungus according to claim 1 is characterized in that: described loam cake is that the loam cake that has the tang pipe is arranged.
5. production method of the present invention is characterized in that:
(1) gets the culturing bottle that cleans up and nutrient solution packed into;
(2) add a cover and insert pipeline;
(3) culturing bottle is carried out high-temperature sterilization with ordinary method;
(4) open the sealing cover that connects, gets the bacterial classification mouth of pipe, connect spawn culture 24～72 hours, covered loam cake, and carried out 5～30 minutes oxygenation in wherein per 6～10 hours;
(5) after cultivation is finished culturing bottle is put into box bucket, open and connect, get bacterial classification seal for pipe joints mouth, insert the bacterial classification rifle.
(6) to cultivation bag injecting fluid bacterial classification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610081643 CN1880443A (en) | 2006-05-08 | 2006-05-08 | Portable culture device for liquid strain of edible fungus and using method therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610081643 CN1880443A (en) | 2006-05-08 | 2006-05-08 | Portable culture device for liquid strain of edible fungus and using method therefor |
Publications (1)
| Publication Number | Publication Date |
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| CN1880443A true CN1880443A (en) | 2006-12-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610081643 Pending CN1880443A (en) | 2006-05-08 | 2006-05-08 | Portable culture device for liquid strain of edible fungus and using method therefor |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103275869A (en) * | 2013-06-05 | 2013-09-04 | 浙江大学 | Aerobic thermophilic bacteria enrichment and culture device and its application method |
| CN103834557A (en) * | 2012-11-26 | 2014-06-04 | 唐杰 | Manufacturing method of simple fermentation device for producing liquid strain |
| CN105300737A (en) * | 2015-05-08 | 2016-02-03 | 中国科学院沈阳应用生态研究所 | Long-term in-situ collection method and device for forest tree root exudates |
| CN105722968A (en) * | 2013-11-12 | 2016-06-29 | 美国陶氏益农公司 | Inoculum transfer system |
-
2006
- 2006-05-08 CN CN 200610081643 patent/CN1880443A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103834557A (en) * | 2012-11-26 | 2014-06-04 | 唐杰 | Manufacturing method of simple fermentation device for producing liquid strain |
| CN103275869A (en) * | 2013-06-05 | 2013-09-04 | 浙江大学 | Aerobic thermophilic bacteria enrichment and culture device and its application method |
| CN103275869B (en) * | 2013-06-05 | 2014-10-22 | 浙江大学 | Aerobic thermophilic bacteria enrichment and culture device and its application method |
| CN105722968A (en) * | 2013-11-12 | 2016-06-29 | 美国陶氏益农公司 | Inoculum transfer system |
| CN105300737A (en) * | 2015-05-08 | 2016-02-03 | 中国科学院沈阳应用生态研究所 | Long-term in-situ collection method and device for forest tree root exudates |
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Open date: 20061220 |