CN201309940Y - Flaky fibrous carrier sampler - Google Patents
Flaky fibrous carrier sampler Download PDFInfo
- Publication number
- CN201309940Y CN201309940Y CNU2008201560566U CN200820156056U CN201309940Y CN 201309940 Y CN201309940 Y CN 201309940Y CN U2008201560566 U CNU2008201560566 U CN U2008201560566U CN 200820156056 U CN200820156056 U CN 200820156056U CN 201309940 Y CN201309940 Y CN 201309940Y
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- CN
- China
- Prior art keywords
- pipe
- tube
- sampling
- sheet
- mouth
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a flaky fibrous carrier sampler, which is used for sampling flaky fibrous carriers cultured by a fixed bed of a cell reactor. The sampler mainly comprises a sampling rod, a Y-shaped three- way tube, two glass sampling tubes and a sampling bottle, wherein the glass sampling tubes are respectively connected with openings of a longitudinal branch tube and an inclined branch tube of the Y-shaped three-way tube, the sampling rod is inserted into the main branch tube of the Y-shaped three-way tube and inserted into a longitudinal branch tube communicating with the main branch tube in a line, the opening of the main branch tube is connected with a contracting tube, the lower end of the sampling rod with a sampling probe passing through sampling connecting openings of glass sampling tube openings extends out and positions via a guiding block, the glass sampling tube of the inclined branch tube of the Y-shaped three-way tube is connected with a soft tube, the soft tube is sleeved with a clamp, the lower end of the soft tube extends into the sampling bottle, the inclined branch tube extends out a passage of inclined compressed air blow tube, and the air entering direction of the inclined compressed air blow tube is corresponding to an opening of the inclined compressed air blow tube. The sampler has the advantages of reliable work, easy operation, simple structure and easy disassembly and cleanness, high temperature endurance and pollution prevention, which is capable of taking out one or more pieces of carriers in one time.
Description
Technical field
The utility model relates to the sampler that is used for bio-reactor sheet-like fiber carrier fixed bed pattern cell cultures jar.
Background technology
Greatly before 20 years, U.S. NBS company released sheet-like fiber carrier (
Disk) fixed bed pattern cell cultures jar, this culture tank has many advantages aspect cell cultures, little by little obtain the recognition and acceptance of countries in the world organic sphere, in state-owned very high share of market.But, this fixed bed model animals retort also has many shortcomings, wherein customer responsiveness at most, intensive is, in use can't take a sample (promptly taking out sheet-like fiber carrier), can't count and the kenel observation of growing the cell that attaches on it, and U.S. NBS company does not design corresponding sampling unit so far yet.
The utility model content
At the blank of prior art, the utility model aims to provide a kind of special sheet-like fiber carrier sampler for the design of fixed bed pattern cell cultures jar, makes that sampling becomes possibility.
The technical solution adopted in the utility model is:
A kind of sheet-like fiber carrier sampler, comprise thief rod, Y-type three way type, two glass stopple coupons and sampling jug, described two glass stopple coupons are connected on the vertical smaller branch pipe and the oblique smaller branch pipe mouth of pipe of Y-type three way type, thief rod inserts in vertical smaller branch pipe that the primary branch of Y-type three way type and straight line be communicated with, be connected with expansion pipe on the primary branch mouth of pipe, the thief rod upper end extends in the expansion pipe, the expansion pipe top is provided with the hand of pressing that promotes thief rod, the lower end of thief rod has sampling probe, and to pass sampling connection on the glass stopple coupon mouth of pipe protruding and locate by orienting lug, Y-type three way type is connected with flexible pipe on the glass stopple coupon of its oblique smaller branch pipe, the clip of its break-make of control is housed on the flexible pipe, sampling jug is stretched in the lower end of flexible pipe, oblique smaller branch pipe is upwards drawn the oblique pressurized air gas blow pipe of corresponding its mouth of pipe of one section airintake direction, gas blow pipe links to each other with the disposable micro air filter by pipeline, and air filter is connected to the compressed air source that has pressure regulator valve.
Described orienting lug is fixed on the shaft of thief rod near sampling probe, slides up and down with the sampling connection internal diameter to cooperate.
The vertical smaller branch pipe of described Y-type three way type and the central axis intersection point of oblique smaller branch pipe are not less than the height of the next radius of the primary branch mouth of pipe.
The glass stopple coupon mouth of pipe of described oblique smaller branch pipe has the inclined-plane screens of inwardly takeing in.
The lower end of described flexible pipe has one section stainless steel intubate that stretches into sampling jug.
Described flexible pipe is connected with the glass stopple coupon mouth of pipe by nut, and pad is provided with sealing-ring between the two.
Described vertical smaller branch pipe mouth of pipe is connected with sampling connection by nut, and pad is provided with sealing-ring between the two.
Described sampling connection has the outside screw that is connected with cell cultures jar thief hole, and is respectively equipped with sealing-ring in upper and lower twice ring groove.
Described Y-type three way type is the glass threeway, and the reduction pipe is the silica gel corrugated tube, and flexible pipe is a silicone tube.
The utility model sheet-like fiber carrier sampler reliable operation, simple to operate once can be taken out a slice or several carriers, it is simple in structure, be easy to dismantle, clean, do not have the dead angle, can high temperature, can avoid the danger of polluting.
Description of drawings
Fig. 1 is a structural representation of the present utility model.
Embodiment
Below in conjunction with accompanying drawing the utility model is described in further detail.
Description of reference numerals is as follows:
1---thief rod 2---Y-type three way type
3,3 '---glass stopple coupon 4---sampling jugs
The oblique smaller branch pipe of 5---vertical smaller branch pipe 6---
7---primary branch 8---silica gel corrugated tube
9---pressing hand 10---sampling probe
11---sampling connection 12,12 '---nut
13---orienting lug 14---silicone tube
15---clip 16---compressed air source
17---intubate 18---position, inclined-plane
19---gas blow pipe 20---air filter
As shown in Figure 1, this sheet-like fiber carrier sampler is mainly by thief rod 1, Y-type three way type 2, two glass stopple coupons 3,3 ' and sampling jug 4, Y-type three way type is the glass threeway, two glass stopple coupons 3,3 ' be connected on the mouth of pipe of vertical smaller branch pipe 5 of Y-type three way type and oblique smaller branch pipe 6, vertically the central axis intersection point of smaller branch pipe 5 and oblique smaller branch pipe 6 is not less than the height of the next radius of the primary branch mouth of pipe, so that thief rod is by the smooth and easy transfer between two arms of stretching, thief rod 1 inserts in the primary branch 7 and vertical smaller branch pipe 5 of Y-type three way type, be connected with silica gel corrugated tube 8 on the primary branch mouth of pipe, with secluding air, sealed glass tube, the upper end of thief rod 1 extends in the silica gel corrugated tube 8, what silica gel corrugated tube top was provided with the operation thief rod presses hand 9, the thief rod lower end has sampling probe 10, the sampling connection 11 that passes on the glass stopple coupon mouth of pipe is protruding, vertically the smaller branch pipe mouth of pipe is connected with sampling connection 11 by nut 12, pad is provided with sealing-ring between the two, sampling connection 11 has the outside screw that is connected with cell cultures jar thief hole, and last, be respectively equipped with sealing-ring in the following twice ring groove, thief rod 1 is fixed with on the position near sampling probe 10 with the sampling connection internal diameter and slides up and down the orienting lug 13 that cooperates, with the stationarity of guaranteeing that thief rod moves up and down; Silicone tube 14 by nut 12 ' with glass stopple coupon 3 ' the mouth of pipe be connected, pad is provided with sealing-ring between the two, the formation section of flexibly connecting, which is provided with a clip 15, so that the break-make of control flexible pipe, keep glass stopple coupon cleaning inside, prevent bacterial invasion, the silicone tube lower end has one section stainless steel intubate 17 that stretches into sampling jug 4, the mouth of pipe of glass stopple coupon 3 has the position, inclined-plane 18 of inwardly takeing in, utilize this position to dial the chip carrier that falls to taking out from sampling probe, oblique smaller branch pipe 6 is upwards drawn the oblique gas blow pipe 19 of corresponding its mouth of pipe of one section airintake direction, gas blow pipe links to each other with disposable micro air filter 20 by pipeline, and strainer is connected to the compressed air source 16 that has the pressure regulator valve door.
Specific operation process is as follows:
1) sheet-like fiber carrier sampler is installed, sampling connection is connected with cell cultures jar thief hole, the clip on the sampler silicone tube is clipped, simultaneously air filter is connected to oblique gas blow pipe; Load onto the sampling jug of a sky.Enter next step cell cultivation process after sterilizing together, sterilize with culture tank then.At this moment the sampling jug with the sky behind the silicone tube clip takes off, and changes a sampling jug (note: the stainless steel intubate will be immersed in and spill in the essence) that 25% alcohol is housed;
When 2) taking a sample vial is taken off, replace with the sampling jug (bottle cap opens wide) of other sky, simultaneously compressed air source is linked into the outer end of air filter;
3) the operation thief rod enters the cell cultures jar, get chip carrier by sampling cushion, after upwards being promoted to Y-type three way type intersection, the thief rod that will have chip carrier turns to, stretch into oblique smaller branch pipe, after the sheet microcarrier passes screens, to inhale thief rod extracts out backward, and utilize the screens at Glass tubing mouth of pipe place to dial chip carrier on the sampling probe, then sampling probe is resetted, open compressed air source immediately, unclamp the clip on the silicone tube again, make chip carrier in silicone tube enters sampling jug by the aseptic forced draft that feeds, with clip silicone tube is blocked then, the closes compression air source, the blast main with the respiratory organ outer end disconnects again;
4) after sampling finishes, remove sampling jug, change vial, tighten.
The utility model is not limited to above-mentioned preferred forms, and anyone can draw other various forms of products under enlightenment of the present utility model.Though but on its shape or structure, do any variation, every identical with the utility model or akin technical scheme is all within its protection domain.
Claims (9)
1, a kind of sheet-like fiber carrier sampler, it is characterized in that, comprise thief rod, Y-type three way type, two glass stopple coupons and sampling jug, described two glass stopple coupons are connected on the vertical smaller branch pipe and the oblique smaller branch pipe mouth of pipe of Y-type three way type, thief rod inserts in vertical smaller branch pipe that the primary branch of Y-type three way type and straight line be communicated with, be connected with expansion pipe on the primary branch mouth of pipe, the thief rod upper end extends in the expansion pipe, the expansion pipe top is provided with the hand of pressing that promotes thief rod, the lower end of thief rod has sampling probe, and to pass sampling connection on the glass stopple coupon mouth of pipe protruding and locate by orienting lug, Y-type three way type is connected with flexible pipe on the glass stopple coupon of its oblique smaller branch pipe, the clip of its break-make of control is housed on the flexible pipe, sampling jug is stretched in the lower end of flexible pipe, oblique smaller branch pipe is upwards drawn the oblique pressurized air gas blow pipe of corresponding its mouth of pipe of one section airintake direction, gas blow pipe links to each other with the disposable micro air filter by pipeline, and air filter is connected to the compressed air source that has pressure regulator valve.
2, sheet-like fiber carrier sampler according to claim 1 is characterized in that, described orienting lug is fixed on the shaft of thief rod near sampling probe, slides up and down with the sampling connection internal diameter to cooperate.
3, sheet-like fiber carrier sampler according to claim 1 is characterized in that, the vertical smaller branch pipe of described Y-type three way type and the central axis intersection point of oblique smaller branch pipe are not less than the height of the next radius of the primary branch mouth of pipe.
4, sheet-like fiber carrier sampler according to claim 1 is characterized in that, the glass stopple coupon mouth of pipe of described oblique smaller branch pipe has the inclined-plane screens of inwardly takeing in.
5, sheet-like fiber carrier sampler according to claim 1 is characterized in that, the lower end of described flexible pipe has one section stainless steel intubate that stretches into sampling jug.
6, sheet-like fiber carrier sampler according to claim 1 is characterized in that, described flexible pipe is connected with the glass stopple coupon mouth of pipe by nut, and pad is provided with sealing-ring between the two.
7, sheet-like fiber carrier sampler according to claim 1 is characterized in that, described vertical smaller branch pipe mouth of pipe is connected with sampling connection by nut, and pad is provided with sealing-ring between the two.
8, sheet-like fiber carrier sampler according to claim 1 is characterized in that, described sampling connection has the outside screw that is connected with cell cultures jar thief hole, and is respectively equipped with sealing-ring in upper and lower twice ring groove.
9, sheet-like fiber carrier sampler according to claim 1 is characterized in that, described Y-type three way type is the glass threeway, and the reduction pipe is the silica gel corrugated tube, and flexible pipe is a silicone tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201560566U CN201309940Y (en) | 2008-11-28 | 2008-11-28 | Flaky fibrous carrier sampler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201560566U CN201309940Y (en) | 2008-11-28 | 2008-11-28 | Flaky fibrous carrier sampler |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201309940Y true CN201309940Y (en) | 2009-09-16 |
Family
ID=41107192
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008201560566U Expired - Fee Related CN201309940Y (en) | 2008-11-28 | 2008-11-28 | Flaky fibrous carrier sampler |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201309940Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110938530A (en) * | 2019-12-10 | 2020-03-31 | 华中科技大学 | Adaptor, device and method for continuously acquiring micro tissue blocks |
-
2008
- 2008-11-28 CN CNU2008201560566U patent/CN201309940Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110938530A (en) * | 2019-12-10 | 2020-03-31 | 华中科技大学 | Adaptor, device and method for continuously acquiring micro tissue blocks |
| CN110938530B (en) * | 2019-12-10 | 2021-12-21 | 华中科技大学 | Method for continuously obtaining micro tissue blocks |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090916 Termination date: 20151128 |