CN211255951U

CN211255951U - Multifunctional oxygen-controllable temperature-controlled air pressure-pressurized cell culture device

Info

Publication number: CN211255951U
Application number: CN201921547969.5U
Authority: CN
Inventors: 浠诲嘲; 任峰
Original assignee: Tianjin Stm Biotechnology Co ltd
Current assignee: Tianjin Stm Biotechnology Co ltd
Priority date: 2019-09-18
Filing date: 2019-09-18
Publication date: 2020-08-14
Anticipated expiration: 2029-09-18

Abstract

The utility model provides a multi-functional controllable oxygen accuse temperature atmospheric pressure pressurization cell culture device, including the manometer, the relief pressure valve, cultivate the cover, cultivate jar body, the heating plate, temperature controller takes the valve pipe, advances the oxygen pipe, the air-vent valve, the oxygen jar, the connecting seat stands the seat, the box of keeping in, adjustable portable cultivation frame and recordable observation piece structure. The utility model discloses portable handle, carry put the lid, the shadoof, cultivate the box, the setting of adjusting seat and adjusting bolt is favorable to carrying to put and takes out the cell of cultivation, and the operation of being convenient for can adjust the cultivation interval simultaneously, guarantees to cultivate efficiency; the arrangement of the observation sheet body, the fixing frame and the sealing ring is favorable for observing the culture condition and is convenient for mastering the cell culture condition; the arrangement of the L-shaped placing frame and the recording plate is beneficial to recording the culture state and recording the growth condition of cells in real time.

Description

Multifunctional oxygen-controllable temperature-controlled air pressure-pressurized cell culture device

Technical Field

The utility model belongs to the technical field of cell culture, especially, relate to a multi-functional controllable oxygen accuse temperature atmospheric pressure pressurization cell culture device.

Background

In vitro cell culture is a common technique involved in medical research. It is known that various stimuli such as pressure, oxygen content, temperature, etc. can have important effects on the biological behavior of cells, such as affecting cell proliferation, apoptosis, phenotypic changes, etc. The simple and safe laboratory controllable oxygen temperature control air pressure pressurization cell culture device is beneficial to researching biochemical reactions of various cells under different pressures, anoxic/hypoxic and oxygen-enriched conditions. Various reaction conditions can be applied independently, and the molecular mechanism of cell change under a single factor is researched; the different conditions can be applied simultaneously or sequentially to study the molecular biological characteristics of the cells under the multi-factor change. For example, pulmonary hypertension caused by chronic obstructive pulmonary disease, pulmonary artery endothelial cells, smooth muscle cells and the like are simultaneously influenced by pressure and hypoxia/hypoxia, and the cell culture device can better simulate the cell microenvironment under the disease condition. Other cells directly relevant to pressure and/or oxygen concentration studies are cardiomyocytes, fibroblasts, chondrocytes and osteocytes, renal bladder cells, urothelial cells, ocular epithelial cells, ocular trabecular tissue cells, renal tubular epithelial cells, and the like.

Oxygen, temperature, pressure intervention in vitro cell culture requirements: the oxygen concentration can be controlled at will within the range of experimental design and monitored continuously; the pressure in the culture tank can be controlled within the range of experimental design, and the temperature of the in vitro cell culture environment is consistent with that of a conventional cell culture box for a laboratory. Otherwise, the experimental result is lack of scientificity.

The cell culture devices commonly used in laboratories today are mainly classified into hydraulic pressure and pneumatic pressure.

However, the existing oxygen-controllable temperature-controlled air pressure-pressurized cell culture device also has the problems that the cultured cells are not convenient to take and place, the culture state cannot be observed, and the placing function is not provided.

Therefore, it is necessary to invent a multifunctional cell culture device with controllable oxygen, temperature and pressure.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the utility model provides a multi-functional controllable oxygen accuse temperature atmospheric pressure pressurization cell culture device to there is not being convenient for to get to put and cultivates the cell in solving current controllable oxygen accuse temperature atmospheric pressure pressurization cell culture device, unable observation cultivates the state and does not possess the problem of putting the function. A multifunctional controllable oxygen temperature control air pressure pressurization cell culture device comprises a pressure gauge, a pressure reducing valve, a culture tank cover, a culture tank body, a heating sheet, a temperature controller, a guide pipe with a valve, an oxygen inlet pipe, a pressure regulating valve, an oxygen tank, a connecting seat, a vertical seat, a temporary storage box, an adjustable portable culture rack and a recordable observation sheet structure, wherein the pressure gauge and the pressure reducing valve are sequentially in threaded connection with the upper left side of the culture tank cover from left to right; the culture tank cover is in threaded connection with the upper end of the culture tank body; the heating plate is longitudinally bolted at the middle position of the right wall in the culture tank body; the temperature controller is longitudinally bolted at the middle position of the right side of the culture tank body; the lower longitudinal end of the conduit with the valve is in threaded connection with the inlet of the upper right side of the culture tank cover; one end of the oxygen inlet pipe is in threaded connection with the upper end of the conduit with the valve, and the other end of the oxygen inlet pipe is in threaded connection with the left end of the pressure regulating valve; the right end pipeline of the pressure regulating valve is in threaded connection with the outlet of the upper left end of the oxygen tank; one transverse end of the connecting seat is connected with the upper right side of the culture tank body through a bolt, and the other end of the connecting seat is installed on the upper left side of the oxygen tank through a hoop; the vertical upper end of the vertical seat is connected with the middle position of the lower part of the connecting seat through a bolt; the right surface of the vertical seat is sequentially connected with temporary storage boxes through bolts from top to bottom; the adjustable portable culture rack is connected with the culture tank cover; the structure of the recordable observation piece is connected with the culture tank body; the adjustable portable culture rack comprises a portable handle, a lifting cover, a lifting rod, a culture box, an adjusting seat and an adjusting bolt, wherein the portable handle is connected to the middle position of the upper end of the lifting cover through the bolt; the longitudinal upper end of the lifting rod is connected with the middle position of the upper part of the inner side of the lifting cover through a bolt; the culture boxes are respectively connected to the left side and the right side of the upper part of the adjusting seat through transverse screws; the adjusting seat is horizontally sleeved on the outer surface of the lifting rod from top to bottom in sequence and is fixedly connected and arranged through an adjusting bolt.

Preferably, the recordable observation piece structure comprises an observation piece body, a fixed frame, a sealing ring, an L-shaped placing frame and a recording plate, wherein the observation piece body is embedded in the middle position inside the fixed frame; a sealing ring is arranged around the right surface of the fixed frame; the right lower end of the L-shaped placing frame is connected with the left lower side of the observation sheet body through a screw; the longitudinal lower part of the recording plate is inserted between the L-shaped placing frame and the observation sheet body.

Preferably, the lifting cover is connected to the inlet of the middle position in the culture pot cover in a threaded mode.

Preferably, the culture box adopts a plurality of glass boxes with rectangular longitudinal sections.

Preferably, a rubber sealing strip is arranged at the joint of the lifting cover and the culture tank cover.

Preferably, the observation sheet body specifically adopts a rectangular convex lens.

Preferably, the fixing frame is embedded at the viewing port in the middle of the left wall of the culture tank body in a sealing manner.

Preferably, the sealing ring is a rubber ring.

Preferably, the recording board is a writable white board.

Preferably, the heating plate is connected with the temperature controller through a lead.

Preferably, the heating plate is a PTC heating plate, and the temperature controller is an AI-2070.5 level precision/solid output temperature controller.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the utility model discloses in, portable handle, carry and put the lid, the shadoof, cultivate the box, adjust seat and adjusting bolt's setting is favorable to carrying and puts the cell of taking out the cultivation, the operation of being convenient for can adjust simultaneously and cultivate the interval, guarantees to cultivate efficiency.

2. The utility model discloses in, the observation lamellar body, the setting of fixed frame and sealing washer is favorable to observing the cultivation condition, is convenient for master the cell culture condition.

3. The utility model discloses in, the setting of L type place frame and record board, be favorable to the growth condition of real-time recording culture state and record cell.

4. The utility model discloses in, the connecting seat, the setting of standing the seat and keeping in the box is favorable to temporarily placing the cell of cultivateing, the operation of being convenient for.

5. The utility model discloses in, heating plate and temperature controller's setting, be favorable to the controlled temperature, guarantee cell growth efficiency.

6. The utility model discloses in, the setting of manometer and relief pressure valve, be favorable to controlling detection pressure, guarantee cell survival rate.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

FIG. 2 is a schematic structural view of the adjustable portable culture rack of the present invention.

Fig. 3 is a schematic structural diagram of the recordable observation piece structure of the present invention.

In the figure:

1. a pressure gauge; 2. a pressure reducing valve; 3. culturing a pot cover; 4. culturing the tank body; 5. a heating plate; 6. a temperature controller; 7. a valved conduit; 8. an oxygen inlet pipe; 9. a pressure regulating valve; 10. an oxygen tank; 11. a connecting seat; 12. a vertical seat; 13. a temporary storage box; 14. an adjustable portable culture rack; 141. a carrying handle; 142. lifting and placing a cover; 143. lifting a rod; 144. a culture box; 145. an adjusting seat; 146. adjusting the bolt; 15. a recordable view slice structure; 151. observing the sheet body; 152. a fixing frame; 153. a seal ring; 154. an L-shaped placing frame; 155. and (5) recording the plate.

Detailed Description

The utility model is described in detail with reference to the accompanying drawings, as shown in fig. 1 and fig. 2, a multifunctional controllable oxygen temperature control air pressure cell culture device comprises a pressure gauge 1, a pressure reducing valve 2, a culture tank cover 3, a culture tank body 4, a heating plate 5, a temperature controller 6, a conduit 7 with a valve, an oxygen inlet pipe 8, a pressure regulating valve 9, an oxygen tank 10, a connecting seat 11, a vertical seat 12, a temporary storage box 13, an adjustable portable culture frame 14 and a recordable observation plate structure 15, wherein the pressure gauge 1 and the pressure reducing valve 2 are sequentially connected with the upper left side of the culture tank cover 3 in a threaded manner from left to right; the culture tank cover 3 is in threaded connection with the upper end of the culture tank body 4; the heating plate 5 is longitudinally bolted at the middle position of the right wall in the culture tank body 4; the temperature controller 6 is longitudinally bolted at the middle position of the right side of the culture tank body 4; the lower longitudinal end of the conduit 7 with the valve is in threaded connection with the inlet of the upper right side of the culture tank cover 3; one end of the oxygen inlet pipe 8 is in threaded connection with the upper end of the conduit pipe 7 with the valve, and the other end of the oxygen inlet pipe is in threaded connection with the left end of the pressure regulating valve 9; the right end pipeline of the pressure regulating valve 9 is in threaded connection with the outlet at the upper left end of the oxygen tank 10; one transverse end of the connecting seat 11 is connected with the right upper side of the culture tank body 4 through a bolt, and the other end of the connecting seat is installed on the left upper side of the oxygen tank 10 through a hoop; the vertical upper end of the vertical seat 12 is connected with the middle position of the lower part of the connecting seat 11 through a bolt; the temporary storage boxes 13 are sequentially connected to the right surface of the vertical seat 12 from top to bottom through bolts; the adjustable portable culture rack 14 is connected with the culture tank cover 3; the recordable observation piece structure 15 is connected with the culture tank body 4; the adjustable portable culture rack 14 comprises a portable handle 141, a lifting cover 142, a lifting rod 143, a culture box 144, an adjusting seat 145 and an adjusting bolt 146, wherein the portable handle 141 is connected to the middle position of the upper end of the lifting cover 142 through a bolt; the longitudinal upper end of the lifting rod 143 is bolted at the middle position of the inner upper part of the lifting cover 142; the culture boxes 144 are respectively connected to the left and right sides of the upper part of the adjusting seat 145 by transverse screws; adjusting seat 145 transversely from the top down cup joints the surface at shadoof 143 in proper order to set up through adjusting bolt 146 fixed connection, during the cultivation, loosen adjusting bolt 146 according to the cultivation needs, adjust adjusting seat 145 in the position of shadoof 143 outer wall, after the confirmation position, locking adjusting bolt 146 can cultivate the work through cultivating box 144, can put lid 142 from cultivating cover 3 upper end and take off carrying through handheld hand handle 141, be convenient for carry and put, convenient operation.

In this embodiment, referring to fig. 3, the recordable observation piece structure 15 includes an observation piece body 151, a fixing frame 152, a sealing ring 153, an L-shaped placing frame 154 and a recording plate 155, wherein the observation piece body 151 is embedded in the middle position inside the fixing frame 152; a sealing ring 153 is arranged around the right surface of the fixing frame 152; the right lower end of the L-shaped placing frame 154 is connected with the left lower side of the observation sheet body 151 through a screw; the recording plate 155 is inserted between the L-shaped placing frame 154 and the observation sheet 151 in the longitudinal lower part, the cell culture condition can be observed through the observation sheet 151, the recording plate 155 is taken out, the cell growth condition can be conveniently recorded, the cell growth efficiency is ensured, and good sealing performance can be achieved through the sealing ring 153.

In this embodiment, the lifting cap 142 is screwed to the inlet of the middle part of the inner part of the culture pot cover 3.

In this embodiment, the culture box 144 is specifically a plurality of glass boxes with rectangular longitudinal sections.

In this embodiment, a rubber sealing strip is specifically provided at the joint of the lifting cover 142 and the culture pot cover 3.

In this embodiment, specifically, the observation sheet 151 specifically adopts a rectangular convex lens.

In this embodiment, the fixing frame 152 is specifically embedded in the middle of the left wall of the culture tank 4 in a sealing manner.

In this embodiment, specifically, the sealing ring 153 specifically uses a rubber ring.

In this embodiment, the recording board 155 is a writable whiteboard.

In this embodiment, specifically, the heating sheet 5 and the temperature controller 6 are connected by a wire.

In this embodiment, the heating sheet 5 is specifically a PTC heating sheet, and the temperature controller 6 is specifically an AI-2070.5 level precision/solid state output temperature controller.

Principle of operation

In the utility model, during the cultivation, the adjusting bolt 146 is loosened according to the cultivation requirement, the adjusting seat 145 is positioned on the outer wall of the lifting rod 143, after the position is determined, the adjusting bolt 146 is locked, the cultivation work can be carried out through the cultivation box 144, the lifting cover 142 can be taken down from the upper end of the cultivation tank cover 3 through the handheld handle 141, the lifting and the placing are convenient, the operation is convenient, the cell cultivation condition can be observed through the observation sheet body 151, the recording plate 155 is taken out, the cell growth condition can be conveniently recorded, the cell growth efficiency is ensured, the sealing ring 153 can be used for achieving good sealing performance, the heating temperature of the heating sheet 5 can be controlled in real time through the adjusting temperature controller 6, the valve with the valve guide pipe 7 is opened, the oxygen in the oxygen tank 10 can be led into the inside of the cultivation tank body 4 through the oxygen inlet pipe 8 through the pressure regulating valve 9, the pressure is detected through the pressure gauge 1, the pressure regulating valve, ensuring the survival rate of the cell growth.

Utilize technical scheme, or technical personnel in the field are in the utility model discloses under technical scheme's the inspiration, design similar technical scheme, and reach above-mentioned technological effect, all fall into the utility model discloses a protection scope.

Claims

1. The multifunctional cell culture device is characterized by comprising a pressure gauge (1), a pressure reducing valve (2), a culture tank cover (3), a culture tank body (4), a heating plate (5), a temperature controller (6), a guide pipe (7) with a valve, an oxygen inlet pipe (8), a pressure regulating valve (9), an oxygen tank (10), a connecting seat (11), a vertical seat (12), a temporary storage box (13), an adjustable portable culture rack (14) and a recordable observation piece structure (15), wherein the pressure gauge (1) and the pressure reducing valve (2) are sequentially in threaded connection with the upper left side of the culture tank cover (3) from left to right; the culture tank cover (3) is in threaded connection with the upper end of the culture tank body (4); the heating plate (5) is longitudinally bolted at the middle position of the right wall in the culture tank body (4); the temperature controller (6) is longitudinally bolted at the middle position of the right side of the culture tank body (4); the longitudinal lower end of the conduit (7) with the valve is in threaded connection with the inlet of the upper right side of the culture tank cover (3); one end of the oxygen inlet pipe (8) is in threaded connection with the upper end of the conduit pipe (7) with the valve, and the other end of the oxygen inlet pipe is in threaded connection with the left end of the pressure regulating valve (9); a pipeline at the right end of the pressure regulating valve (9) is in threaded connection with an outlet at the upper left end of an oxygen tank (10); one transverse end of the connecting seat (11) is connected with the right upper side of the culture tank body (4) through a bolt, and the other end of the connecting seat is hooped and installed on the left upper side of the oxygen tank (10); the longitudinal upper end of the vertical seat (12) is connected with the middle position of the lower part of the connecting seat (11) through a bolt; the right surface of the vertical seat (12) is sequentially connected with a temporary storage box (13) through bolts from top to bottom; the adjustable portable culture rack (14) is connected with the culture tank cover (3); the recordable observation piece structure (15) is connected with the culture tank body (4); the adjustable portable culture rack (14) comprises a portable handle (141), a lifting cover (142), a lifting rod (143), a culture box (144), an adjusting seat (145) and an adjusting bolt (146), wherein the portable handle (141) is connected to the middle position of the upper end of the lifting cover (142) through a bolt; the longitudinal upper end of the lifting rod (143) is connected with the middle position of the upper part of the inner side of the lifting cover (142) through a bolt; the culture boxes (144) are respectively connected to the left side and the right side of the upper part of the adjusting seat (145) through transverse screws; the adjusting seat (145) is sleeved on the outer surface of the lifting rod (143) from top to bottom in sequence and is fixedly connected and arranged through an adjusting bolt (146).

2. The multifunctional controlled oxygen temperature-controlled air pressure-pressurized cell culture device according to claim 1, wherein the recordable observation plate structure (15) comprises an observation plate body (151), a fixing frame (152), a sealing ring (153), an L-shaped shelf (154) and a recording plate (155), the observation plate body (151) is embedded in the middle position inside the fixing frame (152); a sealing ring (153) is arranged on the periphery of the right surface of the fixed frame (152); the right lower end of the L-shaped placing frame (154) is connected with the left lower side of the observation sheet body (151) through a screw; the longitudinal lower part of the recording plate (155) is inserted between the L-shaped placing frame (154) and the observation sheet body (151).

3. The multifunctional controlled oxygen temperature-controlled air pressure-pressurized cell culture device according to claim 1, wherein the lifting cover (142) is screwed to the inner middle inlet of the culture pot cover (3).

4. The multifunctional controlled oxygen temperature-controlled air pressure-pressurized cell culture device according to claim 1, wherein the culture box (144) is a plurality of glass boxes having a rectangular longitudinal section.

5. The multifunctional cell culture device with controllable oxygen, temperature and pressure as claimed in claim 1, wherein the joint of the lifting cover (142) and the culture tank cover (3) is provided with a rubber sealing strip.

6. The multifunctional controlled oxygen temperature-controlled air pressure-pressurized cell culture device according to claim 2, wherein the observation plate (151) is a rectangular convex lens.

7. The multifunctional oxygen-controlled temperature-controlled air-pressure-pressurized cell culture device according to claim 2, wherein the fixing frame (152) is hermetically embedded at the viewing port at the middle position of the left wall of the culture tank body (4).

8. The multifunctional controllable oxygen temperature-controlled air pressure-pressurized cell culture device according to claim 2, wherein the sealing ring (153) is a rubber ring.

9. The multifunctional controlled oxygen and air pressure pressurized cell culture device according to claim 2, wherein the recording plate (155) is a writable white plate.

10. The multifunctional controllable oxygen temperature-controlled air pressure-pressurized cell culture device according to claim 1, wherein the heating sheet (5) and the temperature controller (6) are connected by wires.