CN210560489U - Novel culture medium bottle for stem cells - Google Patents
Novel culture medium bottle for stem cells Download PDFInfo
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- CN210560489U CN210560489U CN201921353791.0U CN201921353791U CN210560489U CN 210560489 U CN210560489 U CN 210560489U CN 201921353791 U CN201921353791 U CN 201921353791U CN 210560489 U CN210560489 U CN 210560489U
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- plate
- bottle
- stem cells
- stem cell
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Abstract
The utility model provides a novel culture medium bottle for stem cells, which comprises a bottom plate, a protective pad, a protective plate, a protective shell, a dry and fine gas storage culture bottle structure, a stem cell nutrient solution use record storage bottle structure, a sealing plate, a locking bolt, a protective box, a grab handle, a connecting block, a placing plate and a heating pot, wherein the protective pad is glued on the lower part of the bottom plate; the protection plate is connected to the left side of the upper surface of the bottom plate through bolts; the protective shell is connected to the upper portion of the protective plate through bolts. The arrangement of the placing seat, the supporting rod, the oxygen storage bottle, the flow guide pipe, the sealing cover and the stem cell culture bottle is beneficial to supplying oxygen to the stem cells to be cultured, thereby preventing the stem cells from dying due to oxygen deficiency; the mounting plate, the record piece, the protection rope is placed the shell, the stock solution bottle, and the setting of through-hole and protective cover is favorable to depositing the nutrient solution of cultivateing stem cell to can carry out the record to the in service behavior of nutrient solution.
Description
Technical Field
The utility model belongs to the technical field of cell culture, especially, relate to a novel a culture medium bottle for stem cell.
Background
A stem cell is a cell derived from human tissue, which can proliferate and can differentiate in multiple directions. It has many features including differentiation into various cells, immunoregulation, increasing blood supply, etc.
However, the existing culture medium bottle for stem cells has the problems that stem cells die due to oxygen deficiency, nutrient solution for culturing stem cells is inconvenient to store, the using condition of the nutrient solution is recorded, and the sealing effect of a culture device is poor.
Therefore, it is necessary to invent a new culture medium bottle for stem cells.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model provides a novel a culture medium bottle for stem cell to the culture medium bottle of solving current stem cell is because the oxygen deficiency leads to stem cell death, and inconvenient nutrient solution to cultivateing stem cell deposits and carries out the sealed poor problem of effect of record and culture apparatus to the in service behavior of nutrient solution. A novel culture medium bottle for stem cells comprises a bottom plate, a protective pad, a protective plate, a protective shell, a dry and fine gas storage culture bottle structure, a stem cell nutrient solution use record storage bottle structure, a sealing plate, a locking bolt, a protective box, a grab handle, a connecting block, a placing plate and a heating pot, wherein the protective pad is glued on the lower part of the bottom plate; the protection plate is connected to the left side of the upper surface of the bottom plate through bolts; the protective shell is connected to the upper part of the protective plate through bolts; the dry and fine gas storage culture bottle structure is arranged on the right side of the upper surface of the bottom plate; the stem cell nutrient solution use recording storage bottle structure is arranged on the left side of the front surface of the bottom plate; the sealing plate is placed in the middle of the upper surface of the protective shell; the locking bolt is in threaded connection with the joint of the protective shell and the sealing plate; the protective box is connected to the middle position in the protective shell through a bolt; the grab handle screw is connected to the middle position of the upper surface of the sealing plate; the connecting block is connected to the lower part of the inner side of the protective box through a bolt; the placing plate is connected to the upper part of the connecting block through a bolt; the heating pan is placed on the upper part of the placing plate; the dry and fine gas storage culture bottle structure comprises a placing seat, a support rod, an oxygen storage bottle, a flow guide pipe, a sealing cover and a stem cell culture bottle, wherein the support rod is connected to the middle position of the upper surface of the placing seat through a bolt; the oxygen storage bottle is connected to the upper part of the support rod through a bolt; the guide pipe is in threaded connection with the middle position of the lower part of the left side of the oxygen storage bottle; the sealing cover is in threaded connection with the left side of the bottom of the flow guide pipe; the stem cell culture bottle is connected with the lower part of the sealing cover in a threaded manner.
Preferably, the protective pad is a rubber pad; the bottom of the protective pad is provided with a straight-line-shaped anti-skid line.
Preferably, the protective shell is a trapezoidal stainless steel shell; the outer surface of the protective shell is coated with an anti-rust paint layer.
Preferably, the sealing plate is a stainless steel plate; a rubber sealing strip is arranged at the joint of the bottom plate and the sealing plate; the rubber sealing strip is glued to the lower part of the sealing plate.
Preferably, the protective box is a stainless steel box; the inner side of the protective box is glued with a sponge insulating layer.
Preferably, the oxygen storage bottle is a transparent plastic can.
Preferably, the inner diameter of the flow guide pipe is set to be six millimeters to eight millimeters; the flow guide pipe is a snake-shaped stainless steel hose.
Preferably, the stem cell culture bottle is a transparent glass sheet.
Preferably, the stem cell nutrient solution use recording storage bottle structure comprises a fastening plate, a recording sheet, a protective rope, a storage shell, a liquid storage bottle, a through hole and a protective cover, wherein the recording sheet is glued at the joint of the protective rope and the protective rope; the protection ropes are respectively glued at the four corners of the left side of the front surface of the fastening plate; the placing shell is connected to the lower part of the right side of the fastening plate through bolts; the liquid storage bottles are sequentially inserted into the middle position of the upper part of the inner side of the placing shell from left to right; the through hole is arranged at the upper part of the liquid storage bottle; the protective cover is axially connected to the right side of the upper surface of the liquid storage bottle.
Preferably, the recording sheet is a PVC plastic sheet; the protection rope is specifically a nylon rope.
Preferably, the placing shell is an acrylic plastic shell.
Preferably, the liquid storage bottle specifically adopts an acrylic transparent plastic sheet; a rubber layer is arranged at the joint of the liquid storage bottle and the protective cover; the rubber layer is glued on the inner wall of the protective cover.
Preferably, the placing seat is connected with the bottom plate through bolts; the fastening plate is connected with the bottom plate through bolts.
Preferably, the heating pot is a heating pot with a model of CMT 181.
Compared with the prior art, the beneficial effects of the utility model are that:
1. the utility model discloses in, the seat of placing, branch, the bottle is deposited to oxygen, the honeycomb duct, the setting of sealed lid and stem cell blake bottle is favorable to carrying out the oxygen suppliment to the stem cell that needs were cultivateed to can prevent that the stem cell oxygen deficiency and death.
2. The utility model discloses in, mounting plate, the record piece, the protection rope, the shell is placed, the stock solution bottle, the setting of through-hole and protective cover is favorable to depositing the nutrient solution of cultivateing stem cell to can carry out the record to the in service behavior of nutrient solution.
3. The utility model discloses in, the protecting crust, do thin gas storage blake bottle structure, stem cell nutrient solution record of using stores a bottle structure, the closing plate, locking bolt and protecting box are favorable to sealing the protecting box.
4. In the utility model, the protective pad is a rubber pad; the bottom of protection pad be provided with the antiskid line of a straight line, be favorable to preventing stem cell culture device from sliding on the workstation.
5. In the utility model, the protective shell is made of trapezoidal stainless steel shell; the outer surface of the protective shell is coated with an anti-rust paint layer, which is beneficial to preventing the protective shell from rusting and damaging.
6. In the utility model, the inner diameter of the flow guide pipe is set to be six to eight millimeters; the honeycomb duct specifically adopt snakelike stainless steel hose, be favorable to adjusting the angle of honeycomb duct.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
FIG. 2 is a schematic structural view of the dry and fine air storage culture flask of the present invention.
Fig. 3 is a schematic structural diagram of the structure of the stem cell nutrient solution use recording storage bottle of the present invention.
In the figure:
1. a base plate; 2. a protective pad; 3. a protection plate; 4. a protective shell; 5. a dry and fine gas storage culture bottle structure; 51. a placing seat; 52. a strut; 53. an oxygen storage bottle; 54. a flow guide pipe; 55. a sealing cover; 56. stem cell culture flasks; 6. a stem cell nutrient solution use recording storage bottle structure; 61. a fastening plate; 62. recording sheets; 63. a protective rope; 64. placing the shell; 65. a liquid storage bottle; 66. a through hole; 67. a protective cover; 7. a sealing plate; 8. locking the bolt; 9. a protective box; 10. a handle; 11. connecting blocks; 12. placing the plate; 13. and heating the pan.
Detailed Description
The invention is further described below with reference to the accompanying drawings:
example (b):
as shown in figures 1 to 3
The utility model provides a novel culture medium bottle for stem cells, which comprises a bottom plate 1, a protective pad 2, a protective plate 3, a protective shell 4, a dry and fine air storage culture bottle structure 5, a stem cell nutrient solution use record storage bottle structure 6, a sealing plate 7, a locking bolt 8, a protective box 9, a grab handle 10, a connecting block 11, a placing plate 12 and a heating pot 13, wherein the protective pad 2 is spliced at the lower part of the bottom plate 1; the protection plate 3 is connected to the left side of the upper surface of the bottom plate 1 through bolts; the protective shell 4 is connected to the upper part of the protective plate 3 through bolts; the dry and fine gas storage culture bottle structure 5 is arranged on the right side of the upper surface of the bottom plate 1; the stem cell nutrient solution use recording storage bottle structure 6 is arranged on the left side of the front surface of the bottom plate 1; the sealing plate 7 is placed in the middle of the upper surface of the protective shell 4; the locking bolt 8 is in threaded connection with the joint of the protective shell 4 and the sealing plate 7; the protective box 9 is connected to the middle position inside the protective shell 4 through a bolt; the grab handle 10 is connected to the middle position of the upper surface of the sealing plate 7 through a screw; the connecting block 11 is connected to the lower part of the inner side of the protective box 9 through bolts; the placing plate 12 is connected to the upper part of the connecting block 11 through bolts; the heating pan 13 is arranged on the upper part of the placing plate 12; the dry and fine gas storage culture bottle structure 5 comprises a placing seat 51, a support rod 52, an oxygen storage bottle 53, a guide pipe 54, a sealing cover 55 and a stem cell culture bottle 56, wherein the support rod 52 is connected to the middle position of the upper surface of the placing seat 51 through a bolt; the oxygen storage bottle 53 is connected to the upper part of the support rod 52 through a bolt; the guide pipe 54 is in threaded connection with the middle position of the lower part of the left side of the oxygen storage bottle 53; the sealing cover 55 is in threaded connection with the left side of the bottom of the guide pipe 54; the stem cell culture bottle 56 is screwed to the lower portion of the sealing cap 55.
In the above embodiment, specifically, the protective pad 2 specifically adopts a rubber pad; the bottom of the protective pad 2 is provided with a straight-line-shaped anti-skid line.
In the above embodiment, specifically, the protective shell 4 specifically adopts a trapezoidal stainless steel shell; the outer surface of the protective shell 4 is coated with an anti-rust paint layer.
In the above embodiment, the sealing plate 7 is specifically made of a stainless steel plate; a rubber sealing strip is arranged at the joint of the bottom plate 1 and the sealing plate 7; the rubber sealing strip is glued on the lower part of the sealing plate 7.
In the above embodiment, specifically, the protective box 9 specifically adopts a stainless steel box; the inner side of the protective box 9 is glued with a sponge insulating layer.
In the above embodiment, the oxygen storage bottle 53 is a transparent plastic can.
In the above embodiment, specifically, the inner diameter of the flow guide tube 54 is set to six millimeters to eight millimeters; the flow guide tube 54 is a serpentine stainless steel hose.
In the above embodiment, the stem cell culture bottle 56 is made of a transparent glass plate.
In the above embodiment, specifically, the stem cell nutrient solution use recording storage bottle structure 6 includes a fastening plate 61, a recording sheet 62, a protection rope 63, a placing shell 64, a liquid storage bottle 65, a through hole 66 and a protective cover 67, wherein the recording sheet 62 is glued at the joint of the protection rope 63 and the protection rope 63; the protective ropes 63 are respectively glued to the four corners of the left side of the front surface of the fastening plate 61; the placing shell 64 is connected to the lower part of the right side of the fastening plate 61 through a bolt; the liquid storage bottles 65 are sequentially inserted into the middle position of the upper part of the inner side of the placing shell 64 from left to right; the through hole 66 is arranged at the upper part of the liquid storage bottle 65; the protective cover 67 is coupled to the right side of the upper surface of the liquid storage bottle 65.
In the above embodiment, specifically, the recording sheet 62 specifically adopts a PVC plastic sheet; the protection rope 63 is specifically a nylon rope.
In the above embodiment, specifically, the placing shell 64 is specifically an acrylic plastic shell.
In the above embodiment, specifically, the liquid storage bottle 65 specifically adopts an acrylic transparent plastic sheet; a rubber layer is arranged at the joint of the liquid storage bottle 65 and the protective cover 67; the rubber layer is glued on the inner wall of the protective cover 67.
In the above embodiment, specifically, the placing seat 51 is connected with the bottom plate 1 by bolts; the fastening plate 61 is connected with the bottom plate 1 through bolts.
In the above embodiment, specifically, the heating pan 13 is a heating pan with a model of CMT 181.
Principle of operation
When the utility model is used, firstly, the culture medium bottle of the stem cells is moved to a workbench which needs to culture the stem cells, then the locking bolt 8 is loosened, the handle 10 is held by hand to open the sealing plate 7, then the stem cell culture bottle 56 is taken out to open the stem cell culture bottle 56, then the sealing cover 55 is fixed on the external oxygen cylinder to store the gas in the oxygen storage cylinder 53, then, the stem cells to be cultured are put in the stem cell culture bottle 56, the stem cell culture bottle 56 is screwed to the sealing cap 55, and oxygen is supplied to the stem cells in the stem cell culture bottle 56 by the oxygen in the oxygen storage bottle 53, then the external power supply of the heating pot 13 is switched on, then the heating pot 13 is started to heat and preserve the temperature of the stem cell culture bottle 56, in the process of culturing the stem cells, the stem cells in the stem cell culture bottle 56 are cultured by injecting oxygen gas into the nutrient solution in the solution storage bottle 65.
Utilize technical scheme, or technical personnel in the field are in the utility model discloses under technical scheme's the inspiration, design similar technical scheme, and reach above-mentioned technological effect, all fall into the utility model discloses a protection scope.
Claims (10)
1. The novel culture medium bottle for the stem cells is characterized by comprising a base plate (1), a protective pad (2), a protective plate (3), a protective shell (4), a dry and fine gas storage culture bottle structure (5), a stem cell nutrient solution use record storage bottle structure (6), a sealing plate (7), a locking bolt (8), a protective box (9), a grab handle (10), a connecting block (11), a placing plate (12) and a heating pot (13), wherein the protective pad (2) is glued to the lower part of the base plate (1); the protection plate (3) is connected to the left side of the upper surface of the bottom plate (1) through bolts; the protective shell (4) is connected to the upper part of the protective plate (3) through bolts; the dry and fine gas storage culture bottle structure (5) is arranged on the right side of the upper surface of the bottom plate (1); the stem cell nutrient solution use recording storage bottle structure (6) is arranged on the left side of the front surface of the bottom plate (1); the sealing plate (7) is placed in the middle of the upper surface of the protective shell (4); the locking bolt (8) is in threaded connection with the joint of the protective shell (4) and the sealing plate (7); the protective box (9) is connected to the middle position inside the protective shell (4) through a bolt; the grab handle (10) is connected to the middle position of the upper surface of the sealing plate (7) through a screw; the connecting block (11) is connected to the lower part of the inner side of the protective box (9) through a bolt; the placing plate (12) is connected to the upper part of the connecting block (11) through bolts; the heating pan (13) is arranged on the upper part of the placing plate (12); the dry and fine gas storage culture bottle structure (5) comprises a placing seat (51), a support rod (52), an oxygen storage bottle (53), a guide pipe (54), a sealing cover (55) and a stem cell culture bottle (56), wherein the support rod (52) is connected to the middle position of the upper surface of the placing seat (51) through a bolt; the oxygen storage bottle (53) is connected to the upper part of the support rod (52) through a bolt; the draft tube (54) is in threaded connection with the middle position of the lower part of the left side of the oxygen storage bottle (53); the sealing cover (55) is in threaded connection with the left side of the bottom of the guide pipe (54); the stem cell culture bottle (56) is in threaded connection with the lower part of the sealing cover (55).
2. A new culture medium bottle for stem cells according to claim 1, characterized in that said protective pad (2) is embodied as a rubber pad; the bottom of the protective pad (2) is provided with a straight-line-shaped anti-skid line.
3. A new culture medium bottle for stem cells according to claim 1, characterized in that said protective shell (4) is a trapezoidal stainless steel shell; the outer surface of the protective shell (4) is coated with an anti-rust paint layer.
4. A new culture medium bottle for stem cells according to claim 1, characterized in that said sealing plate (7) is made of stainless steel plate; a rubber sealing strip is arranged at the joint of the bottom plate (1) and the sealing plate (7); the rubber sealing strip is glued to the lower part of the sealing plate (7).
5. A new culture medium bottle for stem cells according to claim 1, characterized in that said protective box (9) is a stainless steel box; the inner side of the protective box (9) is glued with a sponge insulating layer.
6. A new culture medium bottle for stem cells according to claim 1, characterized in that said oxygen storage bottle (53) is embodied in the form of a stand clear plastic jar.
7. The new medium bottle for stem cells according to claim 1, wherein the inner diameter of the flow guide tube (54) is set to six mm to eight mm; the flow guide pipe (54) is a snake-shaped stainless steel hose.
8. A novel culture medium bottle for stem cells as claimed in claim 1, characterized in that said stem cell culture bottle (56) is embodied in the form of a transparent glass plate.
9. The novel culture medium bottle for stem cells as claimed in claim 1, wherein the stem cell nutrient solution use recording storage bottle structure (6) comprises a fastening plate (61), a recording sheet (62), a protective rope (63), a housing (64), a storage bottle (65), a through hole (66) and a protective cover (67), wherein the recording sheet (62) is glued at the joint of the protective rope (63) and the protective rope (63); the protective ropes (63) are respectively glued at the four corners of the left side of the front surface of the fastening plate (61); the placing shell (64) is connected to the lower part of the right side of the fastening plate (61) through a bolt; the liquid storage bottles (65) are sequentially inserted into the middle position of the upper part of the inner side of the placing shell (64) from left to right; the through hole (66) is arranged at the upper part of the liquid storage bottle (65); the protective cover (67) is coupled to the right side of the upper surface of the liquid storage bottle (65).
10. A new culture medium bottle for stem cells according to claim 9, characterized in that said recording sheet (62) is made in particular of PVC plastic sheet; the protection rope (63) is specifically a nylon rope.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921353791.0U CN210560489U (en) | 2019-08-20 | 2019-08-20 | Novel culture medium bottle for stem cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921353791.0U CN210560489U (en) | 2019-08-20 | 2019-08-20 | Novel culture medium bottle for stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210560489U true CN210560489U (en) | 2020-05-19 |
Family
ID=70634219
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921353791.0U Expired - Fee Related CN210560489U (en) | 2019-08-20 | 2019-08-20 | Novel culture medium bottle for stem cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210560489U (en) |
-
2019
- 2019-08-20 CN CN201921353791.0U patent/CN210560489U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200519 Termination date: 20210820 |
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| CF01 | Termination of patent right due to non-payment of annual fee |