CN214881581U - There is not zona pellucida embryo culture apparatus - Google Patents

Abstract

The utility model discloses a non-zona pellucida embryo culture device, which comprises a culture dish and a dish cover; an oil dripping area and a culture area are sequentially arranged in the culture dish from the dish wall to the center direction of the dish; the culture area and the oil dripping area are separated by a first baffle plate; a plurality of circular baffles are arranged in the culture area on the culture dish; a culture chamber is arranged in the circular baffles; the inner part of the dish cover is hollow and used for storing mineral oil, and an annular oil dripping port is arranged on the dish cover and corresponds to the oil dripping area; the oil dripping port is provided with a switch. The utility model discloses need not prick little cave and cultivate the hole and can cultivate no zona pellucida embryo, convenient operation, and the design of ware lid can form thinner mineral oil rete, and mineral oil covers effectually.

Description

There is not zona pellucida embryo culture apparatus

Technical Field

The utility model belongs to the technical field of biological assay equipment, concretely relates to no zona pellucida embryo culture device.

Background

The existing embryo culture method without zona pellucida mainly adopts a micro-hole culture method, namely, an activated embryo is transferred to CO in advance₂The culture box is balanced and is provided with micro holes covered by mineral oil, the micro holes are generally obtained by uniformly and rotatably pricking the bottoms of four hole plates by using embryo gathering needles, and one embryo is placed in each micro hole for culture. Although the method can avoid the problem that the embryos without the zona pellucida are adhered to each other due to the fact that the embryos without the zona pellucida do not exist, the method also needs to prick micro-hole culture holes, and operation is troublesome.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome the technical problems that micro-hole culture methods in the prior art need to be pricked to culture micro-holes and are troublesome to operate, and provides a non-zona pellucida embryo culture device.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a nontransparent zone embryo culture device comprises a culture dish and a dish cover; an oil dripping area and a culture area are sequentially arranged in the culture dish from the dish wall to the center direction of the dish; the culture area and the oil dripping area are separated by a first baffle plate; a plurality of circular baffles are arranged in the culture area on the culture dish; a culture chamber is arranged in the circular baffles; the inner part of the dish cover is hollow and used for storing mineral oil, and an annular oil dripping port is arranged on the dish cover and corresponds to the oil dripping area; the oil dripping port is provided with a switch.

Further, the switch comprises a sealing ring capable of sealing and shielding the oil dripping port and a control piece connected with the sealing ring; the sealing ring is arranged in the dish cover, the control piece is arranged at the top of the dish cover, and the sealing ring is fixedly connected with the control piece.

Furthermore, the outer periphery side and the inner periphery side of the sealing ring are respectively provided with an elastic sealing ring.

Further, the control member comprises a control rod and two connecting rods; the control piece is transversely arranged at the top of the dish cover, two ends of the control piece are respectively connected with one end of a connecting rod, and the other ends of the two connecting rods penetrate into the dish cover and are fixedly connected with the sealing ring.

Further, the bottom of the culture dish is provided with letter marks.

Further, the height of the first baffle is larger than that of the circular baffle.

Furthermore, the top of the dish cover is provided with a closable oil filling hole.

Compared with the prior art, the utility model discloses following beneficial effect has:

the utility model discloses a there is not zona pellucida embryo culture apparatus need not prick little cave and cultivate the hole and can cultivate no zona pellucida embryo, convenient operation. On the quantity of mineral oil, the better more thin under the prerequisite that can cover completely, and the utility model discloses a set up the oil drip mouth in ware lid below, mineral oil in the ware lid drips out from the oil drip mouth, by first shelves board wall to the even flow cover in the culture dish, can form relatively thin mineral oil rete through the volume of instiling of the better control mineral oil of control, mineral oil covers effectually. The utility model discloses a culture chamber in the circular baffle guarantees through the pressure of lateral wall that the embryo can distribute in the center steadily, and it can be better separates the embryo of no zona pellucida. Under the mineral oil cooperation, when the culture dish removed, the embryo can not take place to glue, and the practicality is strong.

Drawings

FIG. 1 is a schematic structural view of a zona pellucida-free embryo culture device of the present invention;

FIG. 2 is a top view of a culture dish without a zona pellucida embryo culture device of the present invention;

FIG. 3 is a top view of a vessel cover without a zona pellucida embryo culture device of the present invention;

in the figure, 1-culture dish, 2-dish cover, 3-oil dripping zone, 4-culture zone, 5-first baffle, 6-circular baffle, 7-mineral oil, 8-sealing ring, 9-control piece, 91-control rod, 92-connecting rod and 10-oil injection hole.

Detailed Description

The present invention will be further described with reference to the accompanying drawings and specific embodiments.

As shown in FIGS. 1-3, the nontransparent zone-free embryo culture device comprises a culture dish 1 and a dish cover 2; an oil dripping area 3 and a culture area 4 are sequentially arranged in the culture dish 1 from the dish wall to the dish center direction; the culture area 4 and the oil dripping area 3 are separated by a first baffle 5; a plurality of circular baffles 6 are arranged in the culture area 4 on the culture dish 1; a culture chamber is arranged in the circular baffles 6; the inner part of the dish cover 2 is hollow and used for storing mineral oil 7, and an annular oil dripping port is arranged on the dish cover 2 and corresponds to the oil dripping area 3; the oil dripping port is provided with a switch.

Preferably, in the present embodiment, the switch includes a sealing ring 8 capable of sealing and shielding the oil dripping port, and a control member 9 connected to the sealing ring 8; the sealing ring 8 is arranged in the dish cover 2, the control piece 9 is arranged at the top of the dish cover 2, and the sealing ring 8 is fixedly connected with the control piece 9. In particular, the control member 9 comprises a control lever 91 and two connecting rods 92; the control piece 9 is transversely arranged at the top of the dish cover 2, two ends of the control piece 9 are respectively connected with one end of the connecting rod 92, and the other ends of the two connecting rods 92 penetrate into the dish cover 2 to be fixedly connected with the sealing ring 8. During the use, accessible control lever 91 lifts sealing ring 8, opens the oil drip mouth, and the mineral oil 7 in the ware lid 2 can be followed the oil drip mouth and dripped out and fall into in the culture dish 1. Mineral oil 7 of this embodiment can be followed the oil drip mouth and dripped to in the oil drip district 3 along the dish wall circumference, when the mineral oil 7 of oil drip district 3 highly is higher than first baffle, mineral oil 7 can be even 4 week side internal propagation in cultivation district, compare in adopting the pipette to absorb mineral oil 7 and directly instil into culture dish 1, this embodiment can effectively avoid mineral oil 7 directly to pile up in one department, also can avoid mineral oil 7 to judge inadequately to mineral oil 7's mobile cover condition when flowing to week side from one department, instil into too much mineral oil 7, mineral oil 7's the volume of instiling into is not well controlled, and this embodiment can be better thinner mineral oil 7 rete, be favorable to observing the cultivation condition.

Preferably, in the present embodiment, the seal ring 8 is provided with elastic seal rings on the outer peripheral side and the inner peripheral side, respectively. The hydraulic fluid port is just sealed to sealing ring 8, and elastic seal can improve sealed effect, when preventing embryo culture, and mineral oil 7 is too much among instiling into culture dish 1, influences embryo's cultivation.

Preferably, in the present embodiment, the bottom of the culture dish 1 is provided with a letter mark. The method can be used for conveniently identifying the embryo culture marks of the culture chamber and conveniently recording the development condition of each embryo in the culture medium.

Preferably, in the present embodiment, the height of the first baffle 5 is greater than the height of the circular baffle. In practical use, the height of the first baffle 5 is slightly higher than that of the circular baffle 6, when the culture dish is used, an embryo is placed in a culture chamber in the circular baffle 6, the embryo is covered with the mineral oil 7, and the height of the first baffle 5 is larger than that of the circular baffle, so that the embryos in the culture dish 1 can be cultured in the same culture environment.

Preferably, in the present embodiment, a closable oil filling hole 10 is provided in the top of the capsule 2. After the mineral oil 7 in the dish cover 2 is used up, the mineral oil 7 can be injected into the surface cover, so that the culture dish 1 can be recycled.

Use the utility model discloses a when no zona pellucida embryo culture device cultivates no zona pellucida embryo, earlier 1 culture dishes into the culture solution after the disinfection, cover dish lid 2 again, lift sealing ring 8 through control lever 91, open the oil drip mouth, mineral oil 7 in the dish lid 2 drips out from the oil drip mouth and falls into culture dish 1 in, when mineral oil 7 of oil drip district 3 highly is higher than first plate washer, mineral oil 7 can be even from 4 week side inwards stretchs in the culture district, after mineral oil 7 covers culture dish 1 completely, but pull-down control lever 91 closes the oil drip mouth is sealed, put into culture dish 1 again after balanced in the incubator with the embryo put into circular plate washer 6 in the dish of cultivateing can cultivate. The utility model discloses need not prick little cave and cultivate the hole and can cultivate no zona pellucida embryo, convenient operation. On the quantity of mineral oil 7, the more thin better under the prerequisite that can cover completely, and the utility model discloses the volume of instiling into of control mineral oil 7 that can be better forms thinner mineral oil 7 rete, and mineral oil 7 covers effectually. The utility model discloses a culture chamber in circular baffle 6 guarantees through the pressure of lateral wall that the embryo can distribute in the center steadily, and it can be better separate no zona pellucida embryo. Under the cooperation of the mineral oil 7, when the culture dish 1 moves, the embryos cannot be adhered, and the practicability is high.

Although the present invention has been described with reference to the above embodiments, it is not intended to limit the present invention, and those skilled in the art can make modifications and improvements without departing from the spirit and scope of the present invention.

Claims (7)

1. A nontransparent zone embryo culture device is characterized by comprising a culture dish and a dish cover; an oil dripping area and a culture area are sequentially arranged in the culture dish from the dish wall to the center direction of the dish; the culture area and the oil dripping area are separated by a first baffle plate; a plurality of circular baffles are arranged in the culture area on the culture dish; culture chambers are arranged in the circular baffles; the inner part of the dish cover is hollow and used for storing mineral oil, and an annular oil dripping port is arranged on the dish cover and corresponds to the oil dripping area; the oil dripping port is provided with a switch.

2. The zona pellucida embryo culture device of claim 1, wherein the switch comprises a sealing ring that can seal and shield the oil dripping port, and a control member connected to the sealing ring; the sealing ring is arranged in the dish cover, the control piece is arranged at the top of the dish cover, and the sealing ring is fixedly connected with the control piece.

3. The zona pellucida-free embryo culture device of claim 2, wherein the outer circumferential side and the inner circumferential side of the sealing ring are provided with elastic sealing rings, respectively.

4. The zona pellucida-free embryo culture device of claim 2, wherein the control member comprises a control lever and two connecting rods; the control piece is transversely arranged at the top of the dish cover, two ends of the control piece are respectively connected with one end of a connecting rod, and the other ends of the two connecting rods penetrate into the dish cover and are fixedly connected with the sealing ring.

5. The zona pellucida-free embryo culture device of claim 1, wherein the bottom of the culture dish is provided with a letter mark.

6. The zona pellucida-free embryo culture device of claim 1 wherein the height of the first barrier is greater than the height of the circular barrier.

7. The zona pellucida-free embryo culture device according to claim 1, wherein a closable oil hole is provided at the top of the dish lid.

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