CN212223009U - Cell culture bottle with function of counting adherent cells - Google Patents
Cell culture bottle with function of counting adherent cells Download PDFInfo
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- CN212223009U CN212223009U CN202020141801.0U CN202020141801U CN212223009U CN 212223009 U CN212223009 U CN 212223009U CN 202020141801 U CN202020141801 U CN 202020141801U CN 212223009 U CN212223009 U CN 212223009U
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Abstract
The utility model relates to a cell culture bottle with count adherent cell function, including cell culture bottle body, cell culture bottle body bottle lid. Five rectangular observation grids are arranged on the back plate surface of the culture bottle body, and A, B, C, D counting areas are arranged in the observation grids. When the total number of the cells in the culture bottle needs to be obtained, A, B, C, D counts the number of the adherent cells in the counting area, and then the total number of the adherent cells in the bottle is calculated through a counting formula. The utility model discloses a can carry out cell count under the condition that does not change adherent cell growth state, be convenient for in the real-time observation bottle cell quantity.
Description
Technical Field
The utility model discloses a belong to biological experiment device field, especially relate to a cell culture bottle with count adherent cell function.
Background
Cell culture is an essential process for the whole bioengineering technology, and the cell culture can make cells obtain a large number of progeny cells under artificially controlled environmental conditions. During the culture of adherent cells, the growth rate of the cells needs to be observed and recorded, and the number of cells in the cell culture flask is estimated before collection. However, in the actual operation process, not only the adherent cells cannot be counted continuously for many times, but also the adherent cells must be digested by trypsin, so that the growth of the cells must be influenced, the statistics of the production rate of the adherent cells cannot be completed, the growth curve of the adherent cells can not be drawn, and the total number of the adherent cells in the cell culture bottle cannot be accurately obtained before collection.
Disclosure of Invention
The utility model aims at providing a cell culture bottle with count adherent cell function in order to overcome the defect that above-mentioned prior art exists, the utility model provides a do not influence adherent cell growth, can't paste wall cell count problem in the blake bottle in real time continuously under the form condition.
The purpose of the utility model can be realized through the following technical scheme:
the cell culture bottle with the function of counting adherent cells comprises a bottle body of the cell culture bottle and a bottle cap of the cell culture bottle; the bottle body is provided with a bottle opening at the contraction part, and comprises a bottle neck, a front plate surface, a rear plate surface, a rectangular observation grid outside the rear plate surface, an A, B, C, D counting area in the rectangular observation grid, a side wall surface and a bottle bottom, wherein the bottle body is in a structure formed by the front plate surface, the rear plate surface, the side wall surfaces at two sides, the bottle bottom and the bottle neck in an integrated manner; the front panel is arranged right in front of the bottle body and corresponds to the rear panel; the side wall surfaces are arranged on the left side and the right side of the bottle body and are connected with the front plate surface and the rear plate surface; the bottle neck is positioned at the contraction part at the upper end of the bottle body, is inclined towards the front panel, is of a cylindrical structure and is provided with threads; the bottle bottom is arranged right below the bottle body; the total area of the rear plate surface is 175cm2The upper half part is trapezoidal, the lower half part is rectangular, the outer side of the rear plate surface is scribed to surround five rectangular observation grids, and the scribing in each rectangular observation grid is divided into A, B, C, D four counting areas; count each rectangular observation gridThe total number of the counted cells in the inner A, B, C, D four counting areas is calculated out by the counting formula; the cell culture bottle cap is a threaded cap and is provided with a vent hole.
The five rectangular observation grids are formed by encircling the outer sides of the rear plate surfaces by scribing lines, and A, B, C, D four counting areas are engraved in the inner scribing lines; the single area of the A, B, C, D counting area is 0.16mm2Is of a square structure and has a total area of 3.2mm2(ii) a The scribed lines in the counting area are divided into 16 small square rectangular grids with the same size; placing the cell culture bottle on a microscope workbench, moving a visual field to a rectangular observation grid, counting the total number of the adherent cells of A, B, C, D four counting areas in each rectangular observation grid, and calculating the total number of the adherent cells in the cell culture bottle by a formula, wherein the calculation formula is that the total number of the adherent cells = the total number of the counted cells multiplied by the total area of the rear plate surface/the total area of the counting area.
Preferably, the A, B, C, D lines of the four counting areas are engraved on the outer side of the rear plate surface of the culture bottle.
Preferably, the bottle body (1) is a transparent polystyrene plate.
Preferably, the thickness of the rear plate surface (4) is larger than that of the front plate surface (3).
Preferably, the inner side wall surface of the bottle of the rear panel (4) is subjected to surface hydrophilic treatment.
The utility model has the advantages that: the culture bottle has simple structure and is specially designed for culture and counting of adherent cells. The line outside the counting area does not influence the growth of the cells adhered to the wall in the culture bottle; the bottle body is a transparent polystyrene plate, so that light can penetrate through the bottle body, and the observation under a microscope is clearer; the rectangular observation grid is beneficial to quickly finding out the counting area, and is convenient to operate and use; the total number of cells in the culture bottle can be calculated by counting the number of the cells adhered to the wall in the counting area, the growth and the shape of the cells are not influenced, and the method is convenient and quick.
Drawings
FIG. 1 is a perspective view of the adherent cell culture bottle of the present invention.
Fig. 2 is a perspective view of the bottle cap of the adherent cell culture bottle of the utility model.
1 is the body, 2 is the bottleneck, 3 is preceding face, 4 is the back face, 5 is the rectangle observation check, 6 is the side wall face, 7 is the count district, 8 is square rectangle check, 9 is the bottle lid, 10 is the air vent, 11 bottle bottoms.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.
A cell culture bottle with function of counting adherent cells, as shown in figure 1 and figure 2, comprises a cell culture bottle body and a cell culture bottle cap; the bottle body comprises a bottle neck, a front panel, a rear panel, a rectangular observation grid outside the rear panel, an A, B, C, D counting area in the rectangular observation grid, a side wall surface and a bottle bottom. The bottle body is a structure which is formed by a front plate surface, a rear plate surface, two side wall surfaces, a bottle bottom and a bottle neck in an integrated manner. The total area of the rear plate surface is 175cm2The upper half part is trapezoidal, the lower half part is rectangular, the scribed lines on the outer side of the rear plate surface surround five rectangular observation grids, and the scribed lines in each rectangular observation grid are divided into A, B, C, D four counting areas; the front panel is arranged right in front of the bottle body and corresponds to the rear panel, the side wall surfaces are arranged at the left side and the right side of the bottle body and are connected with the front panel and the rear panel, the bottle neck is positioned at the contraction part at the upper end of the bottle body, and the front panel is inclined towards the front panel and is of a circular structure; the bottle bottom is arranged right below the bottle body. The cell culture bottle cap is a threaded cap and is provided with a vent hole. A. B, C, D the counting area is a square structure with a single area of 0.16mm2The total area of all counting areas was 3.2mm2The scribe lines in the counting area are divided into 16 small square rectangular grids with equal size.
The culture flask body is transparent polystyrene board, and preceding face is thinner, increases the light transmissivity and makes the microscope count observe clearly. The inner side wall surfaces of the back panel and the bottle body are subjected to surface hydrophilic treatment, and the bottle cap is provided with vent holes, so that the wall-attached growth of cells is facilitated.
Adding a certain amount of culture medium and adherent cells into a culture bottle, horizontally placing the culture bottle, and culturing with the back plate facing downwards. When the required time for the adherent growth of the cells is reached and the adherent cells are counted, the cell culture bottle is placed on a microscope workbench for observation and counting. Is well selected to be suitableThe eyepiece and the objective lens are adjusted to clearly observe adherent cells. Moving the culture bottle to move the visual field to the rectangular observation grids, finding the counting areas, counting the total number of the adherent cells of A, B, C, D four counting areas in each rectangular observation grid, and calculating the total number of the adherent cells in the cell culture bottle by a formula, wherein the calculation formula is that the total number of the adherent cells in the culture bottle = the total number of the counted cells multiplied by the total area of the rear plate surface (175 cm)2) Total area of counting area (3.2 mm)2）。
Claims (7)
1. A cell culture bottle with a function of counting adherent cells is characterized by comprising a bottle body (1) of the cell culture bottle, wherein a bottle mouth is arranged at a contraction part, the bottle body (1) comprises a bottle neck (2), a front plate surface (3), a rear plate surface (4), a rectangular observation grid (5) on the outer side of the rear plate surface, an A, B, C, D counting area (7) in the rectangular observation grid, a side wall surface (6) and a cell culture bottle cap (9), and the bottle body (1) is of a structure which is formed by the front plate surface (3), the rear plate surface (4), the side wall surfaces (6) on two sides and the bottle neck (2) in an integrated mode;
the front plate surface (3) is arranged right in front of the bottle body (1) and corresponds to the rear plate surface (4);
the side wall surfaces (6) are arranged at the left side and the right side of the bottle body (1) and are connected with the front plate surface and the rear plate surface;
the bottle neck (2) is positioned at the upper end contraction part of the bottle body (1), is inclined towards the front panel (3), is of a circular structure and is provided with threads;
the total area of the rear plate surface (4) is 175cm2The upper half part is trapezoidal, the lower half part is rectangular, the outer side of the rear plate surface (4) is scribed to surround five rectangular observation grids (5), and each rectangular observation grid (5) is internally scribed and divided into A, B, C, D four counting areas (7); counting the total number of the counted cells in A, B, C, D four counting areas (7) in each rectangular observation grid (5), and calculating the total number of the adherent cells in the cell culture bottle through a counting formula (11);
the cell culture bottle cap (9) is a threaded cap, and a vent hole (10) is arranged on the cap.
2. The cell culture flask with adherent cell counting function according to claim 1, whereinCharacterized in that the rectangular observation grid (5) is formed by encircling the outer side of the rear plate surface (4) by a scribing line, and A, B, C, D four counting areas (7) are marked on the scribing line in the grid; the A, B, C, D counting area (7) is 0.16mm per single area2Is of a square structure, and the total area of all counting areas is 3.2mm2The inner reticle is divided into 16 small square rectangular grids (8) with equal size.
3. The cell culture bottle with the function of counting adherent cells as claimed in claim 1, wherein the rectangular observation grid (5) is engraved on the outer side of the rear plate surface (4) of the culture bottle, and the engraving line is close to the inner side of the rear plate surface (4).
4. The cell culture bottle with the function of counting adherent cells according to claim 1, wherein the bottle body (1) is a transparent polystyrene plate.
5. The cell culture bottle with the function of counting adherent cells according to claim 1, wherein the thickness of the rear plate surface (4) is larger than that of the front plate surface (3).
6. The cell culture flask according to claim 1, wherein the inner wall surface of the rear plate surface (4) is subjected to surface hydrophilic treatment.
7. The cell culture flask according to claim 1, wherein the count formula (11) is a total number of adherent cells multiplied by a total area of a rear plate surface (175 cm)2) Total area of counting area (3.2 mm)2)。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020141801.0U CN212223009U (en) | 2020-01-22 | 2020-01-22 | Cell culture bottle with function of counting adherent cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020141801.0U CN212223009U (en) | 2020-01-22 | 2020-01-22 | Cell culture bottle with function of counting adherent cells |
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| Publication Number | Publication Date |
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| CN212223009U true CN212223009U (en) | 2020-12-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202020141801.0U Active CN212223009U (en) | 2020-01-22 | 2020-01-22 | Cell culture bottle with function of counting adherent cells |
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| CN (1) | CN212223009U (en) |
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- 2020-01-22 CN CN202020141801.0U patent/CN212223009U/en active Active
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