CN208317773U - A kind of device for harmless quantitative measurement plant callus size - Google Patents

A kind of device for harmless quantitative measurement plant callus size Download PDF

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Publication number
CN208317773U
CN208317773U CN201820206672.1U CN201820206672U CN208317773U CN 208317773 U CN208317773 U CN 208317773U CN 201820206672 U CN201820206672 U CN 201820206672U CN 208317773 U CN208317773 U CN 208317773U
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China
Prior art keywords
bottle
callus
plant callus
culture bottle
size
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CN201820206672.1U
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Chinese (zh)
Inventor
周君浩
刘仁祥
夏志林
田茂竹
王军
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Guizhou University
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Guizhou University
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Priority to CN201820206672.1U priority Critical patent/CN208317773U/en
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model discloses a kind of devices for harmless quantitative measurement plant callus size.Including culture bottle (1), the entire bottom of bottle of culture bottle (1) is equipped with cellular grid, and cellular grid is made of multiple grids (2).The utility model measures plant callus cross section size with bottom millimeter grid line, plant callus height is measured with bottle body longitudinal direction graduation mark, after the culture for carrying out callus in culture bottle, while can guarantee that plant callus is not destroyed or pollutes, harmless quantitative continuous observation callus growing state, it is easy to operate, intuitive is strong, the problem of also optimizing existing callus tissue culture bottle sealability is effective solution to existing quantization plant callus size issue.

Description

A kind of device for harmless quantitative measurement plant callus size
Technical field
The utility model relates to the culture apparatus that one kind can quantify callus size, especially a kind of to be used for harmless quantitative Measure the device of plant callus size.
Background technique
Plant callus is explant under isolated culture condition, and the parenchyma cell group gradually formed, plant is cured Injured tissue has irreplaceable important function in numerous areas such as cell engineering, crop breedings, studies plant callus Growing state is of great significance.Research report was carried out to the growing state of various plants callus currently, having, in the past The research of people is not difficult to find out that there are problems in terms of measurement plant callus growth rate, mainly by plant callus group The method of weighing is knitted to study the growing state of plant callus.Single can disposably break plant callus weighing Bad infection plant's callus, interrupts the continued propagation of plant callus, and plant callus cannot so be observed continuously The growing state of tissue.The method of existing measurement plant callus size is cumbersome, and complicated for operation, measurement result is only planted Object callus weight, data sheet one.So in terms of the growing state of research plant callus, technology at this stage with Method has much room for improvement and innovates.For this purpose, it is proposed that a kind of device of harmless quantitative measurement plant callus size.
Summary of the invention
The purpose of this utility model is to provide it is a kind of can harmless quantitative measurement plant callus size device, with solution The existing measurement plant callus size technology mentioned in certainly above-mentioned background technique exist can not continuous, quantitative measurment plant be cured The problem of injured tissue, and solve the problems, such as that plant callus keeps lossless in measurement process.
The technical solution of the utility model includes: a kind of device for harmless quantitative measurement plant callus size, including The entire bottom of bottle of culture bottle, culture bottle is equipped with cellular grid, and cellular grid is made of multiple grids.
It is previously described in the device of harmless quantitative measurement plant callus size, the culture bottle is transparent material.
It is previously described in the device of harmless quantitative measurement plant callus size, the body of the culture bottle is equipped with Graduation mark.
It is previously described in the device of harmless quantitative measurement plant callus size, the graduation mark is with the bottle of culture bottle Bottom is zero graduation, longitudinally disposed.
It is previously described in the device of harmless quantitative measurement plant callus size, the side length of the grid is 1mm.
It is previously described in the device of harmless quantitative measurement plant callus size, the culture bottle further includes having bottle Lid.
It is previously described in the device of harmless quantitative measurement plant callus size, the bottle cap is equipped with air hole.
It is previously described in the device of harmless quantitative measurement plant callus size, the base of the bottle cap is equipped with rubber Circle.
The utility model has the beneficial effects that measuring plant callus cross section size with grid and graduation mark, training Support bottle in carry out callus culture after, can guarantee that plant callus is not contaminated simultaneously, also harmless quantitative it is lasting The growing state of callus is observed, easy to operate, intuitive is strong, also optimizes asking for existing callus tissue culture bottle sealability Topic is effective solution to existing quantization plant callus size issue.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model;
Fig. 2 is the side view of the utility model;
Fig. 3 is bottle cap schematic diagram;
Fig. 4 is the bottom schematic view of culture bottle.
The label in accompanying drawing is: 1- culture bottle, 2- grid, 3- bottle cap, 4- air hole, 5- graduation mark, 6- rubber ring.
Specific embodiment
The utility model is further described with reference to the accompanying drawings and examples, but is not intended as to the utility model The foundation of limitation.
Embodiment.It is a kind of that for measuring the culture bottle of callus size, composition is as shown in Figs 1-4, including culture bottle 1, The entire bottom of bottle of culture bottle 1 is equipped with cellular grid, and cellular grid is made of multiple grids 2.
The culture bottle 1 is transparent material.Culture bottle 1 can make the plant callus cultivated in bottle using transparent material production Tissue can receive sunlight.
The body of the culture bottle 1 is equipped with graduation mark 5.
The graduation mark 5 is longitudinally disposed using the bottom of bottle of culture bottle 1 as zero graduation.
The side length of the grid is 1mm.
The culture bottle 1 further includes having bottle cap 3.The means such as screw thread carry out activity company can be passed through between culture bottle 1 and bottle cap 3 It connects.
The bottle cap 3 is equipped with air hole 4.The effect of air hole 4 is to make culture bottle 1 connect external environment to be cured to provide plant Injured tissue required air in growth.
The base of the bottle cap 3 is equipped with rubber ring 6.Rubber ring 6 guarantees the leakproofness of culture bottle to avoid the plant in bottle Callus is by external microbial contamination.
The specifically used mode and working principle of the utility model: it when quantitative measurment plant callus size, will cultivate Bottle 1 is placed on horizontal table top and observes its height using graduation mark 5 and record, and picks up culture bottle 1 and observes its bottom, with marking pen mark Plant callus is overlapped with grid 2 out part and counting, amount to the side that entire cellular grid is overlapped with plant callus Lattice number.Method of counting: the half or more that plant callus is overlapped one grid 2 of a grid 2 or coincidence counts a lattice, It is overlapped a grid 2 then without counting less than half.

Claims (2)

1. a kind of device for harmless quantitative measurement plant callus size, it is characterised in that: including culture bottle (1), training The entire bottom of bottle for supporting bottle (1) is equipped with cellular grid, and cellular grid is made of multiple grids (2);On the body of the culture bottle (1) Equipped with graduation mark (5);The graduation mark (5) is longitudinally disposed using the bottom of bottle of culture bottle (1) as zero graduation;The side of the grid (2) A length of 1mm;The culture bottle (1) further includes having bottle cap (3);The bottle cap (3) is equipped with air hole (4);The bottle cap (3) Base is equipped with rubber ring (6).
2. the device according to claim 1 for harmless quantitative measurement plant callus size, it is characterised in that: institute Stating culture bottle (1) is transparent material.
CN201820206672.1U 2018-02-06 2018-02-06 A kind of device for harmless quantitative measurement plant callus size Active CN208317773U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201820206672.1U CN208317773U (en) 2018-02-06 2018-02-06 A kind of device for harmless quantitative measurement plant callus size

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201820206672.1U CN208317773U (en) 2018-02-06 2018-02-06 A kind of device for harmless quantitative measurement plant callus size

Publications (1)

Publication Number Publication Date
CN208317773U true CN208317773U (en) 2019-01-04

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201820206672.1U Active CN208317773U (en) 2018-02-06 2018-02-06 A kind of device for harmless quantitative measurement plant callus size

Country Status (1)

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CN (1) CN208317773U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115104534A (en) * 2022-07-19 2022-09-27 赣南师范大学 Non-damage physical disinfection and separation method after plant callus contamination

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115104534A (en) * 2022-07-19 2022-09-27 赣南师范大学 Non-damage physical disinfection and separation method after plant callus contamination

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