CN203794906U - Petri dish capable of counting - Google Patents
Petri dish capable of counting Download PDFInfo
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- CN203794906U CN203794906U CN201420180788.4U CN201420180788U CN203794906U CN 203794906 U CN203794906 U CN 203794906U CN 201420180788 U CN201420180788 U CN 201420180788U CN 203794906 U CN203794906 U CN 203794906U
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- counting
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- culture dish
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model relates to a petri dish capable of counting. The petri dish capable of counting comprises a round dish body and a dish cover matched with the dish body. The dish cover is provided with a handle. The dish cover is made of a transparent material. The inner surface of the dish cover is provided with criss-cross lines which are arranged at equal intervals and perpendicular to one another, and the lines form a plurality of counting squares. The counting squares are distributed in a cross shape around the center of the dish cover, and then five areas, namely the upper area, the lower area, the left area, the right area and the central area are formed. Each area is a counting chamber. The side wall of the dish body is provided with scale lines. The petri dish capable of counting facilitates accurate counting of culture cells and can also display the quantity of culture media.
Description
Technical field
The utility model relates to a kind of medical experimental apparatus, is specifically related to a kind of isarithmic culture dish.
Background technology
In biomedicine experiment, cell cultures is one of experimental technique of commonly using the most.No matter be in former culture inoculation, passage cultivation and the observation to its multiplication capacity, or conventionally all need obtained cell to carry out accurate counting for further experiment provides cell.Be at present all to prepare cell suspension after cell pancreatin is digested to the common method of culturing cell counting, then take a morsel suspension tally or counting rifle count, finally again the cell after counting is carried out to renewed vaccination or the detection for other.All there is following obvious defect in these two kinds of method of counting: 1, after enzymic digestion, can exert an influence to the activity of cell, even cause necrocytosis because digestion time is improper.2, through postdigestive cell inoculate continue cultivate may cause pollution.3, in cell suspension, there is finite concentration pancreatin, during for further experiment, may affect follow-up result of study.
Therefore,, in actual experimental implementation process, need to develop a kind of culture apparatus that can directly culturing cell be counted and accurately be controlled suspension consumption.As the disclosed Germ Colony Selective technology of CN 203212574 U culture dish, although this culture dish can be counted culturing cell, but the reconnaissance position of this culture dish is subject to the restriction of medium square position at the bottom of culture dish body, and this culture dish can not be controlled the amount of substratum in addition.Therefore, need further to improve aspect the control of culturing cell counting and cultivation base unit weight at culture dish.
Summary of the invention
Technical problem to be solved in the utility model is easily to cause the defect such as culturing cell secondary pollution, cell additional injuries, object to be to provide a kind of can to make cell not leave culture dish and realize the cell counter to its counting for existing method for cell count and device.
The isarithmic culture dish of one described in the utility model, the ware lid that comprises circular ware body and coordinate with ware body, this ware covers and is provided with handle.Described ware lid adopts transparent material, and its internal surface is provided with equidistant and orthogonal crisscross lines, forms some counting grids, and the sidewall of described ware body is provided with scale marks.
In order clearly to count, the distribution of described counting grid is that Yi Mingai center is "+" font, forms upper and lower, left and right and totally five regions, center, and each region is a counting cell, and each counting cell grid quantity is totally 9 of 3*3.
In in vitro cell culture experiment, we are by being positioned over this cell culture apparatus under inverted microscope, according to the counting cell in the each orientation of upper cover, count out the number of different sites grid inner cell branch, just can calculate the concrete quantity of cell in culture dish in conjunction with the diameter of culture dish, and the quantity of passing through the handle count enable different sites cell of rotary upper cover side, makes cell counting more objective, accurate.
The utlity model has following advantage:
1, the utility model for existing culturing cell counting need carry out enzymic digestion, renewed vaccination is cultivated and is easily caused culturing cell secondary pollution and affect the shortcoming of cytoactive, the ware body of its design and ware lid can integratedly be realized cell cultures and counting, can realize cell real-time counting is not left to culture environment, effectively avoid secondary pollution and additional injuries.
2, the utility model can be planted in culturing cell in ware body, and according to the scale on the external wall of ware, can judge the add-on of substratum, easy to use, and can carry out preliminary measurement to culturing cell enation and projection length.
3, cover the rotation of handle by ware, the quantity of different sites cell in count enable culture dish, by just can calculate the sum of culture dish inner cell after averaging to counting cells repeatedly, makes cell counting more objective, accurate.
4, apparatus structure is simple, with low cost.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the structural representation of ware lid in Fig. 1;
Fig. 3 is the use state graph of the utility model grid.
Embodiment
Below in conjunction with drawings and Examples, the utility model is described in further detail:
Referring to the isarithmic culture dish of the one shown in Fig. 1 to Fig. 3, the ware lid 2 that it comprises circular ware body 1 and coordinates with ware body, the diameter of ware body 1 is 35mm, the sidewall of this ware lid 2 is provided with handle 21, described ware lid 2 adopts transparent material, its internal surface is provided with equidistant and orthogonal crisscross lines, form some counting grids 22, the distribution of described counting grid 22 is to be "+" font with the center of ware lid 2, form upper and lower, left and right and totally five regions, center, each region is a counting cell.The design having is that each counting cell grid quantity is totally 9 of 3*3, the each 0.3mm of length and width of described each counting cell, count 0.1 millimeter of the each length of grid, wide 0.1 millimeter for 9 in it, area is 0.01 square millimeter, and described each counting cell is a groove.The sidewall of described ware body 1 is provided with scale marks 11, and described sidewall is provided with scale marks 11 and is respectively from bottom to up 0.5ml, 1.0ml, and 1.5ml and 2ml, can show the amount that adds substratum in culture dish substantially.
Principle of work of the present utility model:
By the culturing cell taking out from incubator, according to the scale on ware body sidewall, can judge the add-on of substratum, then under low power lens, find after counting cell, then change high power lens and observe and count.(count block of each counting cell sees that Fig. 2 indicates) all will be observed by each counting cell district, in order to ensure the accuracy of counting, avoids repeat count and error of omission, and in the time of counting, the statistics of the cell to branch on graticule line should have unified regulation.As cell is positioned on the two-wire of grid, when counting, to count or not of reaching the standard grade and roll off the production line, the left line of number or not right lines, to reduce error.Be positioned at Ben Ge reach the standard grade and left line on cell count Ben Ge, these lattice roll off the production line and right line on cell count in accordance with regulations in corresponding lattice.For the cell of division, when neonatal cell, to reach normal cell large time half as large, can be used as two cells and calculate (as shown in Figure 3).After counting, with after 75% alcohol disinfecting culture dish, the cell of observation can be put back to incubator and continue to cultivate or carry out next step experiment.According to the size of our designed counting mechanism, the cell quantity of counting is as follows:
N is: the average cell number in 1 grid
After simplified formula: cell count/ware=N/ counting cell area (0.01mm
2the floorage (diameter 35mm) of) × culture dish
After calculating every ware cell count approximately=N × 105
While carrying out Cells In Vitro counting, the utility model provides a kind of easy culture dish with tally function, the grid that this culture dish ware covers, can maybe need the cell of inoculation to count to cultivating not leaving under culture condition, realizing counting process does not need the digestion of enzyme and leaves culture environment, reduces the middle-chain of experiment and can maintain preferably cytoactive.
Claims (5)
1. an isarithmic culture dish, the ware lid (2) that comprises circular ware body (1) and coordinate with ware body (1), it is characterized in that: described ware lid (2) adopts transparent material, and its internal surface is provided with equidistant and orthogonal crisscross lines, forms some counting grids (22); The sidewall of described ware body (1) is provided with scale marks (11); Described ware lid (2) is provided with handle (21).
2. culture dish according to claim 1, it is characterized in that: the distribution of described counting grid (22) is to be "+" font with the center of ware lid (2), form upper and lower, left and right and totally five regions, center, each region is a counting cell, and each counting cell grid quantity is totally 9 of 3*3.
3. culture dish according to claim 2, is characterized in that: the each 0.3mm of length and width of described each counting cell, count 0.1 millimeter of the each length of grid, wide 0.1 millimeter for 9 in it, and area is 0.01 square millimeter.
4. culture dish according to claim 2, is characterized in that: described each counting cell is a groove.
5. according to the culture dish described in any one of claim 1-4, it is characterized in that: described sidewall is provided with scale marks (11) and is respectively from bottom to up 0.5ml, 1.0ml, 1.5ml and 2ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420180788.4U CN203794906U (en) | 2014-04-15 | 2014-04-15 | Petri dish capable of counting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420180788.4U CN203794906U (en) | 2014-04-15 | 2014-04-15 | Petri dish capable of counting |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203794906U true CN203794906U (en) | 2014-08-27 |
Family
ID=51377139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420180788.4U Expired - Fee Related CN203794906U (en) | 2014-04-15 | 2014-04-15 | Petri dish capable of counting |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203794906U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108102916A (en) * | 2018-02-01 | 2018-06-01 | 江苏省肿瘤防治研究所（江苏省肿瘤医院） | A kind of culture dish of countable cell |
-
2014
- 2014-04-15 CN CN201420180788.4U patent/CN203794906U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108102916A (en) * | 2018-02-01 | 2018-06-01 | 江苏省肿瘤防治研究所（江苏省肿瘤医院） | A kind of culture dish of countable cell |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140827 Termination date: 20170415 |
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| CF01 | Termination of patent right due to non-payment of annual fee |