CN218491776U - Simple cell liquid changing device - Google Patents
Simple cell liquid changing device Download PDFInfo
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- CN218491776U CN218491776U CN202222625750.0U CN202222625750U CN218491776U CN 218491776 U CN218491776 U CN 218491776U CN 202222625750 U CN202222625750 U CN 202222625750U CN 218491776 U CN218491776 U CN 218491776U
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Abstract
The utility model relates to an utensil technical field is assisted in the cell experiment, discloses a lower and simple and easy cell of simple and easy operation of development cost trades liquid device, possesses: a first liquid bottle for containing a cell culture medium to be replaced; a second liquid bottle for holding a PBS to be replaced; a first switch for controlling the outflow or cut-off of the cell culture medium in the first liquid bottle; the second switch is used for controlling the PBS in the second liquid bottle to flow out or cut off; the cell culture bottle is arranged on the lower end surfaces of the first liquid bottle and the second liquid bottle, the waste liquid bottle is arranged on the lower end surface of the cell culture bottle, the second switch is turned on, PBS in the second liquid bottle flows into the cell culture bottle along the bottle wall, the second switch is turned off, and the PBS flows to clean cells by shaking; and opening the first switch to enable the cell culture medium in the first liquid bottle to flow into the cell culture bottle along the bottle wall, and closing the first switch to finish cell liquid change.
Description
Technical Field
The utility model relates to a cell experiment assists utensil technical field, more specifically says, relates to a simple and easy cell trades liquid device.
Background
Necessary operations which are common in the field of basic research of cell culture biomedicine. Because the cell liquid changing operation needs to be repeated for many times in the test operation process, the manual cell liquid changing is the most common mode, and specifically, a liquid drawing pump or a liquid moving machine is used for sucking the cell waste liquid in a cell culture bottle, PBS or DPBS is added for cleaning cells, the PBS or DPBS is sucked out, and the cell culture medium is added. The staff needs to be trained technically in the process, the experiment threshold is high, and accidents such as pollution and the like easily occur in the operation process, so that the experiment success is usually abandoned. Although the fully automatic cell liquid changing machine is available on the market at present, the efficiency of the liquid changing step can be greatly improved, but the cost is high, so that the fully automatic cell liquid changing machine is rarely equipped in laboratories of common colleges and research institutions except cell culture which needs to be repeated in large quantity.
Therefore, development of a cell exchange device with low cost, easy operation and convenient use is a technical problem that needs to be solved urgently by those skilled in the art.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model lies in, because the cost of full-automatic cell liquid changing machine is higher to the aforesaid of prior art, unless need carry out the defect that cell culture is equipped with outward in a large number of repetition in ordinary colleges and universities and research institutions's the laboratory, provide a simple and easy cell liquid changing device that development cost is lower and the simple operation.
The utility model provides a technical scheme that its technical problem adopted is: a simple cell exchange device is constructed, and the device is provided with:
the first liquid bottle is arranged at the top of the liquid changing device and is used for containing a cell culture medium to be changed;
a second liquid bottle for holding a PBS to be replaced;
the first switch is arranged on one side of the lower end face of the first liquid bottle and used for controlling the outflow or cut-off of the cell culture medium in the first liquid bottle;
the second switch is arranged on one side of the lower end face of the second liquid bottle and used for controlling the PBS in the second liquid bottle to flow out or be cut off;
a cell culture bottle which is arranged on the lower end surfaces of the first liquid bottle and the second liquid bottle and is communicated with the first liquid bottle and the second liquid bottle through the first switch and the second switch;
the waste liquid bottle is arranged on the lower end face of the cell culture bottle and is communicated with the cell culture bottle through a third switch;
opening the third switch to enable the waste liquid in the cell culture bottle to flow down along the bottle wall, and closing the third switch after the waste liquid flows out;
turning on the second switch, enabling the PBS in the second liquid bottle to flow into the cell culture bottle along the bottle wall, turning off the second switch, and shaking to enable the PBS to flow to clean cells;
opening the third switch to enable the PBS to flow into the waste liquid bottle, and closing the third switch;
and opening the first switch to enable the cell culture medium in the first liquid bottle to flow into the cell culture bottle along the bottle wall, and closing the first switch to finish cell liquid change.
In some embodiments, the liquid leakage device further comprises a first liquid leakage hole which is arranged at the lower end of the first switch and is communicated with the first switch;
the second liquid leakage hole is arranged at the lower end of the second switch and is communicated with the second switch;
and the third liquid leakage hole is arranged at the lower end of the third switch and is communicated with the third switch.
In some embodiments, the liquid container further comprises a plurality of bottle caps, and the bottle caps are respectively covered on the bottle mouths of the first liquid bottle, the second liquid bottle, the cell culture bottle and the waste liquid bottle.
In some embodiments, a viewing window is provided on the upper end face of the cell culture flask.
In some embodiments, further comprising an air vent disposed on a side of the cell culture flask.
In some embodiments, the telescopic rod is axially arranged on one side of the bottle body and is detachably connected with the bottle body.
In the simple cell liquid changing device of the present invention, by controlling the opening/closing of the first switch, the second switch and the third switch, the sample liquid in the cell culture bottle, the first liquid bottle and the second liquid bottle is correspondingly controlled, the waste liquid is discharged first, then the third switch is turned off, the second switch is turned on, the PBS in the second liquid bottle flows into the cell culture bottle along the bottle wall, the second switch is turned off, and the PBS is shaken to flow and clean the cells; opening the third switch to enable the PBS to flow into the waste liquid bottle, and closing the third switch; and opening the first switch to enable the cell culture medium in the first liquid bottle to flow into the cell culture bottle along the bottle wall, and closing the first switch to finish cell liquid change.
Compared with the prior art, the technical scheme has the following technical effects:
1. the technical scheme is a common material for industrially producing the cell culture bottle, has lower production cost and economic price which is far lower than the price of related products abroad, can meet the experimental requirements of most laboratories in China, and has good universality;
2. the liquid can be changed without opening the cell culture bottle, so that the possibility of pollution is reduced;
3. convenient operation and low use threshold.
Drawings
The invention will be further explained with reference to the drawings and examples, wherein:
FIG. 1 is a schematic structural diagram of an embodiment of a simple cell exchange device provided in the present invention;
fig. 2 is a schematic structural view of another embodiment of the simple cell exchange device provided by the present invention.
Detailed Description
In order to clearly understand the technical features, objects, and effects of the present invention, the embodiments of the present invention will be described in detail with reference to the accompanying drawings.
Example one
As shown in fig. 1, in the first embodiment of the simple cell exchange device of the present invention, the simple cell exchange device includes an observation window 1, a plurality of bottle caps 2, a first liquid bottle 3, a second liquid bottle 4, a cell culture bottle 5, a waste liquid bottle 6, a non-slip pad 7, an air outlet 8, a switch (corresponding to 9a, 9b and 9 c), and a liquid leakage hole (corresponding to 10a, 10b and 10 c).
Wherein, first liquid bottle 3 and second liquid bottle 4 all set up to interior hollow structure, and are equipped with the opening in one end, are used for saving sample liquid respectively.
Specifically, a first liquid bottle 3 is provided at the top of the liquid changing device for containing the cell culture medium to be changed.
The second liquid bottle 4 is arranged on one side of the first liquid bottle 3 in parallel and is used for containing PBS to be replaced.
Further, a first switch 9a is provided on one side of the lower end surface of the first liquid bottle 3, and the outflow or cutoff of the cell culture medium in the first liquid bottle 3 is controlled by controlling the on/off of the first switch 9 a.
The second switch 9b is disposed on one side of the lower end face of the second liquid bottle 4, and is used for controlling the flow of the PBS in the second liquid bottle 4 or stopping the flow of the PBS by controlling the on/off of the second switch 9 b.
The cell culture bottle 5 is a hollow square body, is arranged on the lower end faces of the first liquid bottle 3 and the second liquid bottle 4, and is communicated with the first liquid bottle 3 and the second liquid bottle 4 through a first switch 9a and a second switch 9 b;
the waste liquid bottle 6 is a square body with a hollow structure, is arranged on the lower end face of the cell culture bottle 5 and is communicated with the cell culture bottle 5 through a third switch 9 c.
Specifically, the third switch 9c is opened to allow the waste liquid in the cell culture bottle 5 to flow down along the bottle wall, and after the waste liquid flows out, the third switch 9c is closed;
turning on a second switch 9b, enabling the PBS in the second liquid bottle 4 to flow into the cell culture bottle 5 along the bottle wall, turning off the second switch 9b, and shaking to enable the PBS to flow to clean cells;
opening the third switch 9c to make the PBS flow into the waste liquid bottle 6, and closing the third switch 9c;
the first switch 9a is opened to make the cell culture medium in the first liquid bottle 3 flow into the cell culture bottle 5 along the bottle wall, and then the first switch 9a is closed to finish cell liquid change.
Specifically, the experimenter adds sufficient cell culture medium and PBS into a first liquid bottle 3 and a second liquid bottle 4 which contain the liquid to be changed respectively, the bottle cap 2 does not need to be opened during liquid changing, only the third switch 9c in the cell culture bottle 5 needs to be opened, the waste liquid automatically flows down along the bottle wall, and the third switch 9c is closed after the waste liquid completely flows;
opening the second switch 9b of the second liquid bottle 4 containing the liquid to be changed, the PBS in the second liquid bottle 4 flows into the bottle gently along the bottle wall, then closing the second switch 9b, shaking gently to make the PBS flow to clean the cells, opening the third switch 9c in the cell culture bottle 5 to make the PBS flow into the waste liquid bottle, closing the third switch 9c in the cell culture bottle 5, opening the first switch 9a of the first liquid bottle 3 to make the culture medium flow into the bottle gently along the bottle wall, closing the first switch 9a containing the culture medium bottle, and completing the cell liquid change.
The technical scheme has the following technical effects:
1. the technical scheme is a common material for industrially producing the cell culture bottle, has lower production cost and economic price which is far lower than the price of related products abroad, can meet the experimental requirements of most laboratories in China, and has good universality;
2. the liquid can be changed without opening the cell culture bottle, so that the possibility of pollution is reduced;
3. convenient operation and low use threshold.
In some embodiments, in order to improve the performance of the liquid exchange device, a plurality of weep holes (corresponding to 10a, 10b, and 10 c) may be provided in the liquid exchange device, including a first weep hole 10a, a second weep hole 10b, and a third weep hole 10c.
Specifically, the first leakage hole 10a is arranged at the lower end of the first switch 9a and is communicated with the first switch 9 a;
a second weep hole 10b provided at a lower end of the second switch 9b and communicating with the second switch 9 b;
and a third leakage hole 10c provided at a lower end of the third switch 9c and communicating with the third switch 9 c.
It can be understood that when the first switch 9a, the second switch 9b and the third switch 9c are in the on state, the samples stored in the first liquid bottle 3, the second liquid bottle 4 and the cell culture bottle 5 respectively flow into the corresponding bottles through the first weep hole 10a, the second weep hole 10b and the third weep hole 10c.
In some embodiments, in order to ensure the bottle body to be sealed newly, a plurality of bottle caps 2 may be provided in the liquid changing device, wherein the bottle caps 2 respectively cover the bottle openings of the first liquid bottle 3, the second liquid bottle 4, the cell culture bottle 5 and the waste liquid bottle 6 to effectively seal the bottle bodies.
In some embodiments, in order to ensure the cultivation reliability of the cell culture bottle 5, an observation window 1 may be provided on the upper end surface of the cell culture bottle 5, and the experimenter may observe the cultivation of the cells in the cell culture bottle 5 through the observation window 1.
In some embodiments, the air outlet hole 8 on one side of the cell culture bottle 5 can exhaust air from the cell culture bottle 5 by providing the air outlet hole 8.
In some embodiments, a plurality of anti-slip pads 7 are arranged on the ground side of the waste liquid bottle 6, and the anti-slip pads 7 are arranged to increase the stability of the waste liquid bottle 6.
Example two
As shown in fig. 2, in the second embodiment of the simple cell liquid changing device of the present invention, the simple cell liquid changing device includes each component of the first embodiment, and further includes a telescopic rod 12 connected to the bottle body 11, a telescopic rod 12, and a single chip or a timing device 13, and the above components constitute a manual liquid changing device.
Specifically, the experimenter adds sufficient cell culture medium and sufficient PBS into the first liquid bottle 3 and the second liquid bottle 4 containing the liquid to be replaced, and puts the cell culture box with the single chip microcomputer or the timing device 13 for a set time.
When the liquid is changed, no operation is needed, the telescopic rod 11 automatically opens the third switch 9c in the cell culture bottle 5, the waste liquid automatically flows down along the bottle wall, the third switch 9c is closed after the waste liquid completely flows,
opening the second switch 9b of the second liquid bottle 4 containing the liquid to be changed, enabling PBS in the second liquid bottle 4 to flow into the bottle gently along the bottle wall, then closing the second switch 9b, shaking gently to enable the PBS to flow and clean cells, opening the third switch 9c in the cell culture bottle 5 to enable the PBS to flow into the waste liquid bottle, closing the third switch 9c in the cell culture bottle 5, opening the first switch 9a of the first liquid bottle 3 to enable the culture medium to flow into the bottle gently along the bottle wall, closing the first switch 9a containing the culture medium bottle to finish cell liquid change.
In some embodiments, the telescopic rod 12 is further included, and the telescopic rod 12 is axially arranged on one side of the bottle body and is detachably connected with the bottle body.
The embodiments of the present invention have been described with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, which are only illustrative and not restrictive, and those skilled in the art can make many forms without departing from the scope of the present invention, and these forms are all within the protection scope of the present invention.
Claims (6)
1. A simple cell exchange device is characterized by comprising:
the first liquid bottle is arranged at the top of the liquid changing device and is used for containing a cell culture medium to be changed;
a second liquid bottle for holding a PBS to be replaced;
a first switch provided on one side of a lower end surface of the first liquid bottle, for controlling outflow or cutoff of the cell culture medium in the first liquid bottle;
the second switch is arranged on one side of the lower end face of the second liquid bottle and used for controlling the PBS in the second liquid bottle to flow out or be stopped;
a cell culture bottle which is arranged on the lower end surfaces of the first liquid bottle and the second liquid bottle and is communicated with the first liquid bottle and the second liquid bottle through the first switch and the second switch;
and the waste liquid bottle is arranged on the lower end surface of the cell culture bottle and is communicated with the cell culture bottle through a third switch.
2. The device for changing a liquid for a cell as claimed in claim 1, further comprising
The first liquid leakage hole is arranged at the lower end of the first switch and is communicated with the first switch;
the second liquid leakage hole is arranged at the lower end of the second switch and is communicated with the second switch;
and the third liquid leakage hole is arranged at the lower end of the third switch and is communicated with the third switch.
3. The device for changing a liquid for a cell as claimed in claim 1, further comprising
And the bottle caps are respectively covered on the bottle mouths of the first liquid bottle, the second liquid bottle, the cell culture bottle and the waste liquid bottle.
4. The simple cell exchange device of claim 3,
an observation window is arranged on the upper end surface of the cell culture bottle.
5. The device of claim 3, further comprising a liquid changing device
And the air outlet is arranged on one side of the cell culture bottle.
6. The simple cell liquid changing device according to any one of claims 1 to 5, further comprising a telescopic rod, wherein the telescopic rod is axially arranged on one side of the bottle body and is detachably connected with the bottle body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222625750.0U CN218491776U (en) | 2022-09-30 | 2022-09-30 | Simple cell liquid changing device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222625750.0U CN218491776U (en) | 2022-09-30 | 2022-09-30 | Simple cell liquid changing device |
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| Publication Number | Publication Date |
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| CN218491776U true CN218491776U (en) | 2023-02-17 |
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| CN202222625750.0U Active CN218491776U (en) | 2022-09-30 | 2022-09-30 | Simple cell liquid changing device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117511735A (en) * | 2023-11-09 | 2024-02-06 | 厦门眼科中心有限公司 | Biological cell culture device |
| CN117511735B (en) * | 2023-11-09 | 2024-05-17 | 厦门眼科中心有限公司 | Biological cell culture device |
-
2022
- 2022-09-30 CN CN202222625750.0U patent/CN218491776U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117511735A (en) * | 2023-11-09 | 2024-02-06 | 厦门眼科中心有限公司 | Biological cell culture device |
| CN117511735B (en) * | 2023-11-09 | 2024-05-17 | 厦门眼科中心有限公司 | Biological cell culture device |
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