CN216349734U - Device for collecting fungus fermentation liquor - Google Patents
Device for collecting fungus fermentation liquor Download PDFInfo
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- CN216349734U CN216349734U CN202123035287.6U CN202123035287U CN216349734U CN 216349734 U CN216349734 U CN 216349734U CN 202123035287 U CN202123035287 U CN 202123035287U CN 216349734 U CN216349734 U CN 216349734U
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Abstract
The utility model provides a device for collecting fungus fermentation liquor, which comprises an upper container, a valve and a lower container, wherein the top end of the lower container is connected to the bottom end of the upper container through the valve, a filtering component is arranged on the inner side of the upper part of the upper container, an upper cover is hermetically connected above the upper container, and a lower cover is hermetically connected to the bottom end of the lower container. The device is designed into two independent containers, the middle of the two independent containers are connected through a valve, the two independent containers are respectively used for containing the culture solution and the extracting agent, liquid separation operation is conveniently carried out through valve control in the two independent containers, the filter assembly is made of cellophane material and is high-temperature resistant, and mycelia can permeate the cellophane to absorb nutrition and can also seal the culture solution. Adopt this device to divide liquid, the operation process step is few, can not pollute, and the experiment is high-efficient swift, and the effect is better, easy operation, and efficiency is higher.
Description
Technical Field
The utility model belongs to the technical field of fungus metabolite detection and analysis, and particularly relates to a device for collecting fungus fermentation liquor.
Background
In the fungal metabolite detection and analysis technology, in order to obtain a sterile filtrate (a fungus-free bacterial solution) and add an organic solvent for liquid separation extraction, it is necessary to efficiently obtain the sterile filtrate and a mixed solution thereof. The conventional operation method at present is as follows: culturing fungi in a liquid culture medium, culturing for 4-7 days, filtering with gauze or filter paper sheet under vacuum to obtain filtrate, pouring the filtrate into a funnel, separating, extracting, and performing the next test. There are problems in that: the filter paper is adopted for vacuum filtration, so that the blockage is easy, the filter paper piece can be continuously replaced, the material waste is caused, and the scratch is easy to cause pollution in the experimental process; the gauze is adopted for filtration, if the number of layers of the gauze is large, the blockage is easy to occur, if the number of layers is small, the mycelium is easy to filter into the filtrate, the experiment fails, the rework is needed, the time and the energy are increased, the obtained filtrate needs to be poured into another liquid separating device, the experiment process is large, and the pollution is easily caused in the middle link.
Disclosure of Invention
In order to solve the technical problems, the utility model provides a device for collecting fungus fermentation liquor, which is designed into two independent containers, the middle of the two independent containers is connected through a valve, the two independent containers are respectively used for containing culture solution and an extracting agent, the liquid separation operation is conveniently carried out in the two independent containers through the valve control, a filter assembly adopts cellophane material as a medium for absorbing the culture solution by fungi, and the culture solution can be sealed. Adopt this device to divide liquid, the operation process step is few, can not pollute, and the experiment is high-efficient, the effectual technical problem who solves above-mentioned existence.
The utility model relates to a device for collecting fungus fermentation liquor, which is realized by the following specific technical means:
the device for collecting the fungus fermentation liquor comprises an upper container, a valve and a lower container, wherein the top end of the lower container is connected to the bottom end of the upper container through the valve, a filtering assembly is arranged on the inner side of the upper portion of the upper container, an upper cover is connected to the upper portion of the upper container in a sealing mode, and the bottom end of the lower container is connected with a lower cover in a sealing mode. The device is designed into two independent containers, the middle of the two independent containers are connected through a valve, the two independent containers are respectively used for containing the culture solution and the extracting agent, liquid separation operation is conveniently carried out through valve control in the two independent containers, the filter assembly is made of cellophane material and is high-temperature resistant, and mycelia can permeate the cellophane to absorb nutrition and can also seal the culture solution. Adopt this device to divide liquid, the operation process step is few, can not pollute, and the experiment is high-efficient.
The valve is a ball valve, and a handle is arranged on the side edge of the valve. The valve can be opened or closed by rotating the handle, and the operation is convenient.
The lower part of the upper container is of an inverted cone structure, the upper part of the upper container is of a tubular structure, a first convex ring is fixedly connected to the inner side wall of the upper part of the upper container, and a first sealing ring is arranged above the first convex ring and in the middle of the first convex ring. The filter assembly is made of cellophane material, and has high temperature resistance, and mycelium can absorb nutrition through cellophane, and can also seal culture solution. The outside of the filter assembly is a ring structure coated with a layer of cellophane material. The upper container is filled with culture solution, the liquid level is ensured to be attached to the bottom surface of the filtering component, and the upper cover is covered and screwed tightly for sealing.
The upper cover is connected to the inner side wall of the top end of the upper container through threads, a second sealing ring is arranged between the bottom end face of the upper cover and the filtering assembly, the distance between the top end face of the inner side of the upper cover and the filtering assembly is larger than 100mm, bacteria are connected on the cellophane, and a certain space is reserved above the glass paper to facilitate growth of hyphae.
The upper part of the lower container is of a conical structure, the lower part of the lower container is of a tubular structure, and a second convex ring is fixedly connected to the inner side wall of the bottom of the lower container. The lower container is filled with an extractant, so that the culture solution and the extractant can be conveniently mixed. The valve is opened, the culture solution can automatically flow into the extracting agent to be mixed, the operation in the experimental process is simple and efficient, and the pollution can be effectively avoided.
The lower cover is connected to the inner side wall of the bottom end of the lower container through threads, and a third sealing ring is arranged between the top end face of the lower cover and the second convex ring.
The outer side walls of the upper container and the lower container are fixedly connected through a cylinder, round holes and vertically arranged long round holes are respectively formed in the side walls of the cylinder, and the round holes are located on the outer side of the handle. The handle can be operated through the round hole, and the operation is convenient. The inner conditions of the upper container and the lower container can be conveniently observed through the long round holes, and the upper container and the lower container are made of transparent materials.
The outer side walls of the upper container and the lower container are respectively provided with two rows of first volume scale lines and second volume scale lines, the first volume scale lines and the second volume scale lines and the long circular hole are located in the same direction on a plan view, the first volume scale lines and the second volume scale lines in one row represent the volume when the container is upright, and the first volume scale lines and the second volume scale lines in the other row represent the volume when the container is inverted. The amount of culture solution and extractant can be observed conveniently, the experiment is more accurate, and the liquid separation is more efficient and quick.
The volume of the upper container is smaller than that of the lower container. The culture solution is convenient to pour into the lower container and is contained in a space.
The utility model at least comprises the following beneficial effects:
the device is designed into two independent containers, the middle of the two independent containers are connected through a valve, the two independent containers are respectively used for containing the culture solution and the extractant, the liquid separation operation is conveniently carried out in the two independent containers through the valve control, the filter assembly adopts cellophane material as the medium for the fungus to absorb the culture solution, and the culture solution can be sealed. Adopt this device to divide liquid, the operation process step is few, can not pollute, and the experiment is high-efficient swift, and the effect is better, easy operation, and efficiency is higher.
Description of the drawings:
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a left side view of the present invention;
FIG. 3 is a right side view of the present invention;
fig. 4 is an enlarged view of a part a of the present invention.
Detailed Description
Embodiments of the present invention are described in further detail below by way of examples. The following examples are intended to illustrate the utility model but are not intended to limit the scope of the utility model.
In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "coaxial," "bottom," "one end," "top," "middle," "other end," "upper," "side," "top," "inner," "front," "center," "two ends," and the like are used in an orientation or positional relationship indicated in the drawings for convenience in describing and simplifying the description, and are not intended to indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the utility model. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless explicitly stated or limited otherwise, the terms "mounted," "disposed," "connected," "fixed," "screwed" and the like are to be understood broadly, and for example, they may be fixedly connected, detachably connected, or integrated; can be mechanically or electrically connected; the terms may be directly connected or indirectly connected through an intermediate, and may be communication between two elements or interaction relationship between two elements, unless otherwise specifically limited, and the specific meaning of the terms in the present invention will be understood by those skilled in the art according to specific situations.
Example (b):
the utility model provides a device for collecting fungus fermentation liquor, which comprises an upper container 12, a valve 5 and a lower container 6, wherein the top end of the lower container 6 is connected to the bottom end of the upper container 12 through the valve 5, a filter assembly 2 is arranged on the inner side of the upper part of the upper container 12, an upper cover 1 is hermetically connected above the upper container 12, and the bottom end of the lower container 6 is hermetically connected with a lower cover 8, as shown in attached figures 1-4. The device is designed into two independent containers, the middle of the two independent containers are connected through a valve, the two independent containers are respectively used for containing the culture solution and the extracting agent, liquid separation operation is conveniently carried out through valve control in the two independent containers, the filter assembly is made of cellophane material and is high-temperature resistant, and mycelia can permeate the cellophane to absorb nutrition and can also seal the culture solution. Adopt this device to divide liquid, the operation process step is few, can not pollute, and the experiment is high-efficient.
Further, the valve 5 is a ball valve, and a handle 11 is arranged on the side edge of the valve 5. The valve 5 can be opened or closed by rotating the handle 11, and the operation is convenient.
Furthermore, the lower part of the upper container 12 is of an inverted cone structure, the upper part of the upper container 12 is of a tubular structure, a convex ring I3 is fixedly connected to the inner side wall of the upper part of the upper container 12, the filter assembly 2 is positioned above the convex ring I3, and a sealing ring I14 is arranged in the middle of the filter assembly. The filter assembly 2 adopts cellophane material as medium for fungus to absorb culture solution, and can also seal the culture solution. The outside of the filter assembly 2 is an annular structure which is wrapped with a layer of cellophane material. The upper container 12 is filled with culture solution, the liquid level is ensured to be attached to the bottom surface of the filter component 2, and the upper cover 1 is covered and screwed tightly for sealing.
Furthermore, the upper cover 1 is connected to the inner side wall of the top end of the upper container 12 through threads, a second sealing ring 15 is arranged between the bottom end face of the upper cover 1 and the filtering component 2, the distance between the top end face of the inner side of the upper cover 1 and the filtering component 2 is larger than 100mm, bacteria are connected on the cellophane, and a certain space is reserved above the cellophane to facilitate the growth of hyphae.
Furthermore, the upper part of the lower container 6 is of a conical structure, the lower part of the lower container is of a tubular structure, and a second convex ring 9 is fixedly connected to the inner side wall of the bottom of the lower container 6. The lower container 6 is filled with an extractant, so that the culture solution and the extractant can be conveniently mixed. The valve 5 is opened, the culture solution can automatically flow into the extracting agent to be mixed, the operation in the experimental process is simple and efficient, and the pollution can be effectively avoided.
Further, the lower cover 8 is connected to the inner side wall of the bottom end of the lower container 6 through threads, and a third sealing ring 7 is arranged between the top end face of the lower cover 8 and the second convex ring 9.
Further, the outer side walls of the upper container 12 and the lower container 6 are fixedly connected through a cylinder 13, a circular hole 10 and a vertically arranged long circular hole 4 are respectively arranged on the side walls of the cylinder 13, and the circular hole 10 is positioned on the outer side of the handle 11. The handle 11 can be operated through the circular hole 10, and the operation is convenient. The oblong holes 4 can conveniently observe the internal conditions of the upper container 12 and the lower container 6, and the upper container 12 and the lower container 6 are both made of transparent materials.
Furthermore, two rows of first volume scale lines 16 and second volume scale lines 17 are respectively arranged on the outer side walls of the upper container 12 and the lower container 6, the first volume scale lines 16 and the second volume scale lines 17 and the long circular hole 4 are located on the same direction in a plan view, the first volume scale lines 16 and the second volume scale lines 17 in one row represent the volume when the container is upright, and the first volume scale lines 16 and the second volume scale lines 17 in the other row represent the volume when the container is inverted. The amount of culture solution and extractant can be observed conveniently, the experiment is more accurate, and the liquid separation is more efficient and quick.
Further, the volume of the upper container 12 is smaller than that of the lower container 6. The culture solution is convenient to pour into the lower container and is contained in a space.
A liquid separation method for a device for collecting fungus fermentation liquid comprises the following steps: firstly, a valve 5 is in a closed state; secondly, putting the culture solution into an upper container 12, then putting the filter component 2 on the culture solution to ensure that the liquid level is attached to the bottom surface of the filter component 2, covering an upper cover 1, and screwing and sealing the upper cover; thirdly, the device is inverted, the extractant is filled into the lower container 6 according to the required amount, and the upper cover 8 and the lower cover 8 are covered and screwed tightly for sealing; fourthly, the device is turned upside down and placed upright for culture; fifthly, after the culture is carried out for a period of time required by the experiment, opening a valve 5, enabling the culture solution to flow into a lower container 6 to be mixed with an extracting agent to obtain a mixed solution, closing the valve 5, and cleaning an upper container 12; sixthly, oscillating and uniformly mixing the mixed solution, and standing the device upside down; and seventhly, opening the lower cover 8 to take the upper layer solution, and opening the valve 5 to take the lower layer solution.
The device is designed into two independent containers, the middle of the two independent containers are connected through a valve, the two independent containers are respectively used for containing the culture solution and the extracting agent, liquid separation operation is conveniently carried out through valve control in the two independent containers, the filter assembly is made of cellophane material and is high-temperature resistant, and mycelia can permeate the cellophane to absorb nutrition and can also seal the culture solution. Adopt this device to divide liquid, the operation process step is few, can not pollute, and the experiment is high-efficient swift, and the effect is better, easy operation, and efficiency is higher.
The utility model is not described in detail, but is well known to those skilled in the art.
The embodiments of the present invention have been presented for purposes of illustration and description, and are not intended to be exhaustive or limited to the utility model in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiment was chosen and described in order to best explain the principles of the utility model and the practical application, and to enable others of ordinary skill in the art to understand the utility model for various embodiments with various modifications as are suited to the particular use contemplated.
Claims (9)
1. An apparatus for collecting fungal fermentation broth, comprising: the filter assembly comprises an upper container (12), a valve (5) and a lower container (6), wherein the top end of the lower container (6) is connected to the bottom end of the upper container (12) through the valve (5), a filter assembly (2) is arranged on the inner side of the upper part of the upper container (12), an upper cover (1) is hermetically connected above the upper container (12), and a lower cover (8) is hermetically connected to the bottom end of the lower container (6).
2. An apparatus for collecting fungal fermentation broth according to claim 1, wherein: the valve (5) is a ball valve, and a handle (11) is arranged on the side edge of the valve (5).
3. An apparatus for collecting fungal fermentation broth according to claim 1, wherein: the lower part of going up container (12) is the back taper structure, and upper portion is the tubular structure, fixedly connected with bulge loop one (3) on the upper portion inside wall of last container (12), and filter assembly (2) are located bulge loop one (3) top and centre and have sealing washer one (14).
4. An apparatus for collecting fungal fermentation broth according to claim 1, wherein: the upper cover (1) is connected to the inner side wall of the top end of the upper container (12) through threads, a second sealing ring (15) is arranged between the bottom end face of the upper cover (1) and the filtering component (2), and the distance between the top end face of the inner side of the upper cover (1) and the filtering component (2) is larger than 100 mm.
5. An apparatus for collecting fungal fermentation broth according to claim 1, wherein: the upper part of the lower container (6) is of a conical structure, the lower part of the lower container is of a tubular structure, and a second convex ring (9) is fixedly connected to the inner side wall of the bottom of the lower container (6).
6. An apparatus for collecting fungal fermentation broth according to claim 1, wherein: the lower cover (8) is connected to the inner side wall of the bottom end of the lower container (6) through threads, and a sealing ring III (7) is arranged between the top end face of the lower cover (8) and the convex ring II (9).
7. An apparatus for collecting fungal fermentation broth according to claim 1, wherein: the outer side walls of the upper container (12) and the lower container (6) are fixedly connected through a cylinder body (13), the side walls of the cylinder body (13) are respectively provided with a round hole (10) and a vertically arranged long round hole (4), and the round hole (10) is positioned on the outer side of the handle (11).
8. An apparatus for collecting fungal fermentation broth according to claim 1, wherein: the outer side walls of the upper container (12) and the lower container (6) are respectively provided with two rows of volume scale marks I (16) and two rows of volume scale marks II (17), the volume scale marks I (16) and the volume scale marks II (17) and the long round hole (4) are located on the same direction on a plan view, one row of volume scale marks I (16) and the volume scale marks II (17) represent the volume when the container is upright, and the other row of volume scale marks I (16) and the volume scale marks II (17) represent the volume when the container is inverted.
9. An apparatus for collecting fungal fermentation broth according to claim 1, wherein: the volume of the upper container (12) is smaller than that of the lower container (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123035287.6U CN216349734U (en) | 2021-12-06 | 2021-12-06 | Device for collecting fungus fermentation liquor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123035287.6U CN216349734U (en) | 2021-12-06 | 2021-12-06 | Device for collecting fungus fermentation liquor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216349734U true CN216349734U (en) | 2022-04-19 |
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ID=81158426
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123035287.6U Active CN216349734U (en) | 2021-12-06 | 2021-12-06 | Device for collecting fungus fermentation liquor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216349734U (en) |
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2021
- 2021-12-06 CN CN202123035287.6U patent/CN216349734U/en active Active
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