CN111635859A - Rapid reaction device and method for stem cell extraction - Google Patents
Rapid reaction device and method for stem cell extraction Download PDFInfo
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- CN111635859A CN111635859A CN202010569624.0A CN202010569624A CN111635859A CN 111635859 A CN111635859 A CN 111635859A CN 202010569624 A CN202010569624 A CN 202010569624A CN 111635859 A CN111635859 A CN 111635859A
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 83
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 44
- 238000000605 extraction Methods 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 21
- 238000005485 electric heating Methods 0.000 claims description 18
- 230000036647 reaction Effects 0.000 claims description 18
- 230000003321 amplification Effects 0.000 abstract description 5
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 5
- 230000036632 reaction speed Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 4
- 230000000694 effects Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 210000004504 adult stem cell Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M23/52—Mobile; Means for transporting the apparatus
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
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Abstract
The invention belongs to the technical field of stem cells, and particularly relates to a rapid reaction device and a rapid reaction method for stem cell extraction, which comprise a box body, wherein a fixed plate is fixedly connected inside the box body, the upper end of the fixed plate is fixedly connected with a circulating fan and an air cooler, the bottom end of the fixed plate is fixedly connected with a filter plate, the filter plate is lapped with the air cooler, a sliding groove is formed in the box body, and a sliding block is slidably connected in the sliding groove; according to the invention, the optimal time for the rapid response of the stem cells can be screened by the box body, the extraction of the stem cells with high speed at the later stage is facilitated, the amplification window is arranged, so that a user can directly and externally observe the stem cells in the incubator in the box body in an amplification way, the air cooler is arranged to cool the temperature in the box body through the through hole at the bottom end of the box body and the filter plate, the extraction delay of the stem cells caused by overhigh temperature in the box body can be prevented, and the circulating fan is arranged to circulate the gas in the box body, so that the reaction speed of the stem cells is increased.
Description
Technical Field
The invention belongs to the technical field of stem cells, and particularly relates to a rapid reaction device and a rapid reaction method for stem cell extraction.
Background
Stem cells are a type of pluripotent cells with self-replication ability, which can be differentiated into various functional cells under certain conditions, and are classified into embryonic stem cells and adult stem cells according to the development stage of the stem cells, and are classified into three types according to the development potential of the stem cells: the stem cells are cells which are not fully differentiated and immature, have the potential function of regenerating various tissues and organs and human bodies, are called as universal cells in the medical field, are extracted from human placentas in medical treatment, and need to be cut, reacted and filtered in the extraction process of the stem cells, however, the stem cells and medicaments are placed in a container to automatically react in the conventional filtering equipment, and the reaction is usually carried out at a normal temperature in the reaction process, but the temperatures used in different seasons are different, so that the rapid reaction device and the rapid reaction method for extracting the stem cells are provided for better controlling the reaction temperature of the stem cells.
Disclosure of Invention
To solve the problems set forth in the background art described above. The invention provides a rapid reaction device and a rapid reaction method for stem cell extraction, which have the characteristics of selecting the optimal stem cell reaction temperature and controlling the uniform stem cell reaction temperature in the later stage.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a stem cell draws and uses quick reaction unit and method, includes the box, the box is inside fixedly connected with fixed plate, fixed plate upper end fixedly connected with circulating fan and air-cooler, the fixed plate bottom end fixedly connected with filter, and filter and air-cooler overlap joint, the spout has been seted up in the box, sliding connection has the slider in the spout, slider side fixedly connected with backup pad, be provided with first controller, second controller, third controller and fourth controller in the backup pad, fixedly connected with four U type pieces in the backup pad, be provided with electric heating wire in the U type piece.
Preferably, a clamping groove is formed in the upper end of the U-shaped block, a clamping block is clamped in the clamping groove, a U-shaped cover plate is fixedly connected to the upper end of the clamping block, and a temperature sensor is fixedly connected to the bottom ends of the supporting plate and the fixing plate.
Preferably, the bottom of box has seted up the through-hole, the box has the chamber door through hinge swing joint, be provided with the magnifying window on the chamber door, chamber door fixed surface is connected with the handle, the chamber door passes through sealed pad and chamber door overlap joint, the bottom fixedly connected with universal wheel of box.
Preferably, the surface of the box door is fixedly connected with an operation panel, a microprocessor is arranged in the operation panel, and the microprocessor is electrically connected with the air cooler, the electric heating wire, the circulating fan, the temperature sensor, the first controller, the second controller, the third controller and the fourth controller through wires.
Preferably, the rapid reaction device for stem cell extraction is used in a method comprising the following steps;
the method comprises the following steps: firstly, placing preselected stem cells into a culture device, and placing the culture device into a U-shaped block in a box body;
step two: the first controller, the second controller, the third controller and the fourth controller are controlled to work through the microprocessor by the operation panel, and the temperature of the corresponding electric heating wire in the U-shaped block is set through the first controller, the second controller, the third controller and the fourth controller;
step three: observing the condition of stem cell reaction in the corresponding incubator through an amplification window on the surface of the box door after a certain time, and determining the optimal temperature of the stem cell reaction;
step four: taking out the first batch of stem cells, putting the second batch of stem cells into the box body, and simultaneously adjusting the electric heating wires corresponding to the first controller, the second controller, the third controller and the fourth controller to a uniform temperature;
step five: when the temperature sensor in the box body detects that the temperature is too high, the microprocessor can automatically control the air cooler and the circulating fan to work and control the temperature of stem cell reaction in the box body;
step six: after the stem cell reaction is completed, the reacted stem cells are removed by opening.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, the optimal time for screening the stem cells to react quickly is set through the box body, the extraction of the stem cells with high speed at the later stage is also facilitated, the amplification window is set, so that a user can observe the stem cells in the incubator in the box body in an amplification mode, the air cooler is set, so that the temperature in the box body can be cooled through the through hole at the bottom end of the box body and the filter plate, the extraction delay of the stem cells caused by overhigh temperature in the box body can be prevented, the filter plate is set, so that the influence of air on the extraction of the stem cells is reduced, the circulating air blower is set, so that the gas in the box body can be circulated, the temperature of the box body can be controlled conveniently, and.
2. According to the invention, the first controller, the second controller, the third controller and the fourth controller are arranged under the action of the operation panel and the microprocessor, so that the optimal reaction time of stem cells can be screened out, the temperature in the box body can be controlled better in the later period, the stem cell reaction efficiency is improved, the electric heating wire can be arranged to control the stem cell reaction temperature in the incubator, the stem cells can be better reacted in the later period, the sliding groove and the sliding block are arranged to facilitate the taking-out of the supporting plate from the box body, the corresponding incubator can be replaced conveniently, the U-shaped cover plate is arranged to compress the incubator in the U-shaped block under the matching of the clamping groove and the clamping block, and the using effect of the device can be better under the mutual matching of the structures.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic front view of the present invention;
FIG. 2 is a schematic front view of the present invention;
FIG. 3 is an enlarged schematic view of the structure A of the present invention;
FIG. 4 is a schematic diagram of the system of the present invention;
in the figure: 1. a box body; 2. a hinge; 3. an amplifying window; 4. a gasket; 5. a box door; 6. an operation panel; 7. a microprocessor; 8. a handle; 9. an air cooler; 10. a universal wheel; 11. a through hole; 12. a filter plate; 13. a fixing plate; 14. a support plate; 15. a circulating fan; 16. a temperature sensor; 17. a chute; 18. a slider; 19. a first controller; 20. a U-shaped block; 21. an electric heating wire; 22. a U-shaped cover plate; 23. a card slot; 24. a clamping block; 25. a second controller; 26. a third controller; 27. and a fourth controller.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
Referring to fig. 1-4, the present invention provides the following technical solutions: a rapid reaction device and a method for stem cell extraction comprise a box body 1, wherein the box body 1 is arranged to screen the optimal time for rapid reaction of stem cells, and to facilitate the extraction of the stem cells with high speed in the later period, a fixed plate 13 is fixedly connected inside the box body 1, a circulating fan 15 and an air cooler 9 are fixedly connected to the upper end of the fixed plate 13, a filter plate 12 is fixedly connected to the bottom end of the fixed plate 13, the filter plate 12 is arranged to filter the gas entering the box body 1, so as to reduce the influence of the air on the extraction of the stem cells, the filter plate 12 is in lap joint with the air cooler 9, the air cooler 9 is arranged to cool the temperature inside the box body 1 through a through hole 11 at the bottom end of the box body 1 and the filter plate 12, so as to prevent the stem cells from being extracted and delayed due to overhigh temperature inside the box body 1, a chute 17 is arranged in the box body 1, convenient incubator that corresponds of changing, sliding connection has slider 18 in spout 17, slider 18 side fixedly connected with backup pad 14, be provided with first controller 19, second controller 25, third controller 26 and fourth controller 27 in the backup pad 14, through setting up first controller 19, second controller 25, third controller 26 and fourth controller 27 and can select the best reaction time of stem cell under operating panel 6 and microprocessor 7's effect, the better control box 1 internal temperature in later stage of being convenient for improves the efficiency of stem cell reaction, four U type pieces 20 of fixedly connected with in backup pad 14, be provided with electric heating wire 21 in the U type piece 20, set up the temperature that the stem cell reacted in electric heating wire 21 can the control incubator, enable the better reaction of later stage stem cell.
Specifically, a clamping groove 23 is formed in the upper end of the U-shaped block 20, a clamping block 24 is clamped in the clamping groove 23, a U-shaped cover plate 22 is fixedly connected to the upper end of the clamping block 24, the U-shaped cover plate 22 is arranged to be capable of compressing the incubator in the U-shaped block 20 under the matching of the clamping groove 23 and the clamping block 24, and a temperature sensor 16 is fixedly connected to the bottom ends of the supporting plate 14 and the fixing plate 13.
Specifically, through-hole 11 has been seted up to the bottom of box 1, box 1 has chamber door 5 through hinge 2 swing joint, be provided with magnifying glass 3 on the chamber door 1, set up magnifying glass 3 and be convenient for the user of service personnel externally directly to the interior incubator of box 1 in the stem cell enlarged the observation, 5 fixed surface of chamber door are connected with handle 8, chamber door 5 is through sealed 4 and 5 overlap joints of chamber door, the bottom fixedly connected with universal wheel 10 of box 1.
Specifically, 5 fixed surface of chamber door is connected with operating panel 6, be provided with a microprocessor 7 in the operating panel 6, microprocessor 7 passes through wire and air-cooler 9, electric heating wire 21, circulating fan 15, temperature sensor 16, first controller 19, second controller 25, third controller 26 and fourth controller 27 electric connection, sets up circulating fan 15 and can circulate the gas in the box 1, is convenient for control the temperature of box 1, improves the speed of stem cell reaction.
Specifically, the use method of the rapid reaction device for stem cell extraction is as follows;
the method comprises the following steps: firstly, placing preselected stem cells into an incubator, and placing the incubator into a U-shaped block 20 in the box body 1;
step two: the operation panel 6 controls the first controller 19, the second controller 25, the third controller 26 and the fourth controller 27 to work through the microprocessor 7, and the temperature of the corresponding electric heating wire 21 in the U-shaped block 20 is set through the first controller 19, the second controller 25, the third controller 26 and the fourth controller 27;
step three: observing the condition of stem cell reaction in the corresponding incubator through the amplifying window 3 on the surface of the box door 5 after a certain time, and determining the optimal stem cell reaction temperature;
step four: taking out the first batch of stem cells, putting the second batch of stem cells into the box body 1, and simultaneously adjusting the electric heating wires 21 corresponding to the first controller 19, the second controller 25, the third controller 26 and the fourth controller 27 to be at a uniform temperature;
step five: when the temperature sensor 16 in the box body 1 detects that the temperature is too high, the microprocessor 7 can automatically control the cold air blower 9 and the circulating air blower 15 to work and control the temperature of stem cell reaction in the box body 1;
step six: after the stem cell reaction is completed, the reacted stem cells are removed by opening.
The working principle and the using process of the invention are as follows: when the device is used, a user puts stem cells to be extracted in a reaction manner into the incubator, adds corresponding stem cell extraction agents into the incubator, then puts the incubator into the U-shaped block 20, presses the incubator tightly by the U-shaped cover plate 22 under the matching of the clamping groove 23 and the clamping block 24, then closes the box door 5, controls the first controller 19, the second controller 25, the third controller 26 and the fourth controller 27 to work through the microprocessor 7 by the operating panel 6, then starts the electric heating wires 21 in the corresponding U-shaped block 20 by the first controller 19, the second controller 25, the third controller 26 and the fourth controller 27, adjusts the temperatures of the first controller 19, the second controller 25, the third controller 26 and the fourth controller 27 corresponding to the electric heating wires 21 by the operating panel 6, and observes the extraction condition of the stem cells in the incubator in the box body 1 by the amplifying window 3 after a certain time, the optimal extraction temperature is determined in the process of stem cell extraction, then the box door 5 is opened to take out all the incubators, then, the stem cells are replaced with new stem cells and extracted, and when the stem cells are put into the incubator again, the first controller 19, the second controller 25, the third controller 26 and the fourth controller 27 are started to work through the microprocessor 7 via the operation panel 6, and the electric heating wires 21 corresponding to the first controller 19, the second controller 25, the third controller 26 and the fourth controller 27 are adjusted to a uniform temperature, the stem cells are extracted while the temperature sensor 16 inside the housing 1 transmits the temperature inside the housing 1 to the operation panel 6, when the temperature in the inner box body 1 is overhigh, the microprocessor 7 controls the air cooler 9 and the circulating fan 15 to work, the air cooler 9 exchanges heat with the inside of the box body 1 through the through hole 11 at the bottom end of the box body 1 and the filter plate 12, and the temperature inside the box body 1 is controlled by the temperature sensor 16 and the microprocessor 7 for extraction.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. A rapid reaction device and a method for extracting stem cells comprise a box body (1), and are characterized in that: the improved air cooler is characterized in that a fixing plate (13) is fixedly connected to the inside of the box body (1), a circulating fan (15) and an air cooler (9) are fixedly connected to the upper end of the fixing plate (13), a filter plate (12) is fixedly connected to the bottom end of the fixing plate (13), the filter plate (12) is in lap joint with the air cooler (9), a sliding groove (17) is formed in the box body (1), a sliding block (18) is connected to the sliding groove (17) in a sliding mode, a supporting plate (14) is fixedly connected to the side face of the sliding block (18), a first controller (19), a second controller (25), a third controller (26) and a fourth controller (27) are arranged in the supporting plate (14), four U-shaped blocks (20) are fixedly connected to the supporting plate (14), and electric heating wires (21.
2. The rapid reaction device and method for stem cell extraction according to claim 1, wherein: draw-in groove (23) have been seted up to the upper end of U type piece (20), block (24) have been connect to the joint in draw-in groove (23), the upper end fixedly connected with U apron (22) of block (24), backup pad (14) and fixed plate (13) bottom fixedly connected with temperature sensor (16).
3. The rapid reaction device and method for stem cell extraction according to claim 1, wherein: through-hole (11) have been seted up to the bottom of box (1), box (1) has chamber door (5) through hinge (2) swing joint, be provided with magnifying window (3) on chamber door (1), chamber door (5) fixed surface is connected with handle (8), chamber door (5) are through sealed pad (4) and chamber door (5) overlap joint, the bottom fixedly connected with universal wheel (10) of box (1).
4. The rapid reaction device and method for stem cell extraction according to claim 3, wherein: the refrigerator door is characterized in that an operation panel (6) is fixedly connected to the surface of the refrigerator door (5), a microprocessor (7) is arranged in the operation panel (6), and the microprocessor (7) is electrically connected with the air cooler (9), the electric heating wire (21), the circulating fan (15), the temperature sensor (16), the first controller (19), the second controller (25), the third controller (26) and the fourth controller (27) through wires.
5. The rapid reaction device and method for stem cell extraction according to claim 1, wherein: the use method of the rapid reaction device for extracting the stem cells comprises the following steps;
the method comprises the following steps: firstly, placing preselected stem cells into an incubator, and placing the incubator into a U-shaped block (20) inside a box body (1);
step two: then, the operation panel (6) controls the first controller (19), the second controller (25), the third controller (26) and the fourth controller (27) to work through the microprocessor (7), and the temperature of the corresponding electric heating wire (21) in the U-shaped block (20) is set through the first controller (19), the second controller (25), the third controller (26) and the fourth controller (27);
step three: observing the condition of stem cell reaction in the corresponding incubator through the amplifying window (3) on the surface of the box door (5) after a certain time, and determining the optimal stem cell reaction temperature;
step four: taking out the first batch of stem cells, putting the second batch of stem cells into the box body (1), and simultaneously adjusting the electric heating wires (21) corresponding to the first controller (19), the second controller (25), the third controller (26) and the fourth controller (27) to be at a uniform temperature;
step five: when a temperature sensor (16) in the box body (1) detects that the temperature is too high, the microprocessor (7) can automatically control the air cooler (9) and the circulating fan (15) to work and control the temperature of stem cell reaction in the box body (1);
step six: after the stem cell reaction is completed, the reacted stem cells are removed by opening.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010569624.0A CN111635859A (en) | 2020-06-20 | 2020-06-20 | Rapid reaction device and method for stem cell extraction |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010569624.0A CN111635859A (en) | 2020-06-20 | 2020-06-20 | Rapid reaction device and method for stem cell extraction |
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| CN111635859A true CN111635859A (en) | 2020-09-08 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117586871A (en) * | 2024-01-19 | 2024-02-23 | 江苏和泽干细胞基因工程有限公司 | Temperature control equipment for cell culture |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207581831U (en) * | 2017-12-01 | 2018-07-06 | 重庆金时代生物技术有限公司 | A kind of miniaturization cell transport and culture apparatus |
| CN208440638U (en) * | 2018-04-08 | 2019-01-29 | 南京惠正干细胞生物科技有限公司 | A kind of stem cell prevention disease equipment |
-
2020
- 2020-06-20 CN CN202010569624.0A patent/CN111635859A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207581831U (en) * | 2017-12-01 | 2018-07-06 | 重庆金时代生物技术有限公司 | A kind of miniaturization cell transport and culture apparatus |
| CN208440638U (en) * | 2018-04-08 | 2019-01-29 | 南京惠正干细胞生物科技有限公司 | A kind of stem cell prevention disease equipment |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117586871A (en) * | 2024-01-19 | 2024-02-23 | 江苏和泽干细胞基因工程有限公司 | Temperature control equipment for cell culture |
| CN117586871B (en) * | 2024-01-19 | 2024-04-30 | 江苏和泽干细胞基因工程有限公司 | Temperature control equipment for cell culture |
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Application publication date: 20200908 |