CN116004359A

CN116004359A - Incubator for cell culture

Info

Publication number: CN116004359A
Application number: CN202310149647.XA
Authority: CN
Inventors: 李然; 李凯; 唐昊天
Original assignee: Jiangsu Selpu Biotechnology Co ltd
Current assignee: Jiangsu Selpu Biotechnology Co ltd
Priority date: 2023-02-22
Filing date: 2023-02-22
Publication date: 2023-04-25
Anticipated expiration: 2043-02-22
Also published as: CN116004359B

Abstract

The application discloses incubator for cell culture mainly comprises the atomizer, supply tube, feed division room, torsion bar, supply hole I and supply hole II, has the supply tube through the output fixed mounting of atomizer, the one end fixed mounting of supply tube has the feed division room, and the quantity of feed division room is two, installs respectively on the position that is close to the division board in culture room left side chamber and right side chamber bottom, and supply hole II is in crisscross state with supply hole I under the initial state. The atomized nutrient solution enters the culture dish positioned at the top of the bearing plate, so that the nutrient solution is continuously added to the culture dish, so that in an initial state, too much nutrient solution is not needed to be added in the culture dish, the problem of too high cell growth and division speed caused by eutrophication in the culture dish is avoided, the growth and division speed of cells is controlled, and an experimenter can better observe and intervene in the cell growth and division process, and the reliability of the device in the use process is improved.

Description

Incubator for cell culture

Technical Field

The application relates to the technical field of microbial cultivation, in particular to an incubator for cell culture.

Background

In microorganism culture operation, all can adopt the incubator, place microorganism in culture dish or blake bottle to add the nutrient solution in inside, place culture dish and blake bottle in the incubator again, set up suitable temperature and humidity, thereby cultivate microorganism, traditional incubator comprises box, the support frame, control by temperature change equipment and humidity control equipment, the preparation has all played important effect in the aspect such as biochemistry, medicine, the incubator of this structure has simple structure, convenient to use and low cost, is the most extensive incubator of cell culture of application scope at present.

Although the conventional incubator has the advantages, a certain limitation still exists in the actual use process, in order to ensure the normal growth and division of microorganisms, a large amount of nutrient solution is added into a culture dish or a culture flask, but the cell quantity in an initial state is small, the cell wall is damaged by the large amount of nutrient solution due to the problem of osmotic pressure, the cell death is caused by the large amount of nutrient solution, the cell growth and division speed is too high, and the colony formation speed is too high, so that the control is difficult.

Disclosure of Invention

The application adopts the following technical scheme: an incubator for cell culture comprising a culture chamber: the front movable mounting of cultivateing the room has sealed door, cultivate the bottom fixed mounting of room inner chamber and have the device that lifts up, cultivate all fixed mounting has the division board on the position that room inner chamber bottom is close to both sides, the division board divide into left chamber, well chamber and right chamber with cultivateing the inner chamber of room, supply port I has been seted up to one side of division board, supply port I distributes with linear array's mode, fixed mounting has trigger piece I on the position that division board one side is close to the top, fixed mounting has feeding mechanism on the position that the left chamber of cultivateing the room is close to the division board, the top fixed mounting of lifting up the device has loading attachment, cultivate the position that room inner chamber bottom is located the loading attachment both sides on fixed mounting have trigger device.

Further, the lifting device comprises an air feeder, an air supply pipe is fixedly arranged at the output end of the air feeder, an air pressure bin is fixedly arranged at one end of the air supply pipe, the air pressure bin is fixedly arranged at the bottom of the inner cavity of the culture chamber, telescopic sleeves are fixedly arranged at the positions, close to four corners, of the top of the air pressure bin and are communicated with the inner cavity of the air pressure bin, telescopic rods are movably sleeved in the telescopic sleeves, and the telescopic rods are fixedly connected with the bottom of the bearing device.

Further, the feeding device comprises an atomizer, a feeding pipe is fixedly arranged at the output end of the atomizer, a material distributing chamber is fixedly arranged at one end of the feeding pipe, the number of the material distributing chambers is two, the material distributing chambers are respectively arranged at the positions of the bottoms of the left cavity and the right cavity of the culture chamber, which are close to the partition plate, and are connected through a communicating pipe, a material distributing pipe is fixedly arranged at the top of the material distributing chamber and distributed in a linear array mode, the material distributing pipe is fixedly connected with the partition plate, and the material distributing pipe is communicated with the inner cavity of the material distributing chamber.

Further, the distributing pipe corresponds to and is communicated with the supply hole I.

Further, the bearing device comprises a bottom plate, the both sides at bottom plate top are all fixed mounting has the curb plate, supply hole II has been seted up on the position that one side of curb plate is close to the top, supply hole II distributes with linear array's mode, the cooperation groove has been seted up at the top of curb plate, and the cooperation groove corresponds with trigger piece I, one side fixed mounting of curb plate has spacing spout, the loading board has been placed at the top of spacing spout, the intercommunicating pore has been seted up on the position that the loading board top is close to both sides.

Further, the supply hole II corresponds to the supply hole I, and the supply hole II and the supply hole I are in a staggered state in the initial state.

Further, trigger device includes the stroke sleeve, stroke sleeve fixed mounting is located the position of lifting device both sides in cultivateing room inner chamber bottom, one side fixed mounting at stroke sleeve top has the gear chamber, the inside activity of gear chamber has cup jointed drive gear, drive gear's one end fixed mounting has the torsion bar, the inside movable mounting of stroke sleeve has the stroke pole, and one side and the drive gear of stroke pole mesh, the top fixed mounting of stroke pole has trigger piece II.

Further, the output end of the trigger block I is connected with the input end of the air supply machine in a signal connection mode.

Further, the output end of the trigger block II is connected with the input end of the sealing door in a signal connection mode.

The application has the following beneficial effects.

1. The atomizing opportunity atomizes and carries the inside to the feed divider room through the supply pipe to enter into the inside of carrying device through torsion bar, supply hole I and supply hole II, the nutrient solution of atomizing can enter into the culture dish that is located the loading board top this moment, thereby constantly add the nutrient solution to the culture dish, make under initial condition, do not need to add too much nutrient solution in the culture dish, avoid eutrophication in the culture dish to cause the too fast problem of cell growth division rate, thereby the growth division rate of control cell, thereby make the experimenter can be better carry out better observation and intervention to the cell growth division in-process, the reliability of the device in the use has been improved.

2. Along with the growth division of cells in the culture dish for the total weight of culture dish and cells constantly increases, thereby make the loading attachment follow vertical decurrent orientation under the effect that total weight increases and remove, along with the removal of loading attachment, supply hole I and supply hole II between be close to each other, thereby make the intercommunication degree between supply hole I and the supply hole II improve, thereby make more atomized nutrient solution enter into the inside of loading attachment through torsion bar, supply hole I and supply hole II, guarantee that the inside cell quantity of culture dish increases the in-process nutrient solution that this device provided also constantly improves, guarantee that the atomized nutrient solution that provides of atomizer can keep on the best level, avoid causing the waste of nutrient solution, the use cost of this device has been reduced.

3. Along with the cell growth division, the bearing device can continuously move downwards along the vertical direction for the bottom of bearing device is continuously close to trigger device, until the bottom of bearing device contacts with trigger block II, be connected with the input of sealing door through the mode of signal connection this moment through the output of trigger block II, thereby make the sealing door open, and remind experimenter cell culture to accomplish, simultaneously, thereby drive gear through rotating torsion bar and rotate, mesh with drive gear through one side of stroke pole, thereby control the height of stroke pole, and adjust the weight when bearing device and trigger block II contact, make experimenter can adjust the height of stroke pole according to the demand, the practicality of the device has been improved.

4. Before the use, accessible air feeder fills the inside in atmospheric pressure storehouse, the telescopic link stretches out from telescopic tube's inside under the effect of atmospheric pressure this moment, thereby with carrying device whole jack-up, until the cooperation groove contacts with trigger block I, the output through trigger block I is connected with the input of air feeder through signal connection's mode this moment, make carrying device can make the air feeder stop to the inside injection gas in atmospheric pressure storehouse after reaching the top, guarantee to make carrying device in the biggest eminence under initial condition, and the inside atmospheric pressure in atmospheric pressure storehouse also reaches the weight that supports carrying device just, guarantee that the device is more accurate to the weight control of cell in the culture dish, the practicality of the device has further been improved.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the disclosure and together with the description, serve to explain the principles of the disclosure.

The disclosure may be more clearly understood from the following detailed description taken in conjunction with the accompanying drawings in which:

FIG. 1 is a schematic diagram of the structure of the present invention;

FIG. 2 is a schematic top view of the structure of the present invention;

FIG. 3 is a schematic view of a cross-section in direction A of FIG. 2, which is a structural diagram of the present invention;

FIG. 4 is a schematic view of a cross-section in the direction B of FIG. 2, which is a structural diagram of the present invention;

FIG. 5 is a schematic view of the internal structure of the present invention;

FIG. 6 is a schematic diagram of the front view of the internal structure of the present invention;

FIG. 7 is a schematic view of a cross section in the direction C of FIG. 6, which is a structural diagram of the present invention;

FIG. 8 is a schematic view of a cross-section in direction D of FIG. 6, which is a block diagram of the present invention;

FIG. 9 is a schematic view of the section in the E direction of FIG. 6 showing the structure of the present invention;

FIG. 10 is a schematic view of a structural lift device of the present invention;

FIG. 11 is a schematic view of a structural load bearing apparatus according to the present invention;

FIG. 12 is a schematic top view of a structural load bearing apparatus of the present invention;

FIG. 13 is a schematic view of a cross-section in the F direction of FIG. 12 showing the structure of the present invention;

FIG. 14 is a schematic view of a structural activation device according to the present invention;

FIG. 15 is a schematic front view of a trigger device according to the present invention;

FIG. 16 is a schematic view of a cross section in the G direction of FIG. 15, which is a block diagram of the present invention;

FIG. 17 is a schematic view of a section in the H direction of FIG. 15 showing the structure of the present invention.

In the figure: 1. a culture chamber; 2. sealing the door; 3. lifting the device; 31. a gas supply machine; 32. an air supply pipe; 33. an air pressure bin; 34. a telescoping sleeve; 35. a telescopic rod; 4. a partition plate; 5. a supply hole I; 6. a trigger block I; 7. a supply device; 71. an atomizer; 72. a supply pipe; 73. a material distributing chamber; 74. a material dividing pipe; 8. a carrying device; 81. a bottom plate; 82. a side plate; 83. a supply hole II; 84. a mating groove; 85. limiting sliding grooves; 86. a carrying plate; 87. a communication hole; 9. a triggering device; 91. a travel sleeve; 92. a gear chamber; 93. a transmission gear; 94. a torsion bar; 95. a travel bar; 96. triggering block II.

Detailed Description

The following description of the embodiments of the present application will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all, of the embodiments of the present application. All other embodiments, which can be made by one of ordinary skill in the art based on the embodiments herein without making any inventive effort, are intended to be within the scope of the present application.

Example 1

The utility model provides a incubator for cell culture, including cultivateing room 1, cultivate the positive movable mounting of room 1 has sealing door 2, cultivate the bottom fixed mounting of room 1 inner chamber and have lifting device 3, cultivate the equal fixed mounting of position that is close to both sides in room 1 inner chamber bottom and have division board 4, division board 4 divide into left chamber with cultivate the inner chamber of room 1, well chamber and right side chamber, supply port I5 has been seted up to one side of division board 4, supply port I5 distributes with linear array's mode, fixed mounting has trigger piece I6 on the position that division board 4 one side is close to the top, fixed mounting has feeding device 7 on the position that the left chamber of cultivate room 1 is close to division board 4, lifting device 3's top fixed mounting has carrier 8, cultivate the position that room 1 inner chamber bottom is located carrier 8 both sides on fixed mounting have trigger device 9.

The lifting device 3 comprises an air feeder 31, an air supply pipe 32 is fixedly arranged at the output end of the air feeder 31, an air pressure bin 33 is fixedly arranged at one end of the air supply pipe 32, the air pressure bin 33 is fixedly arranged at the bottom of the inner cavity of the culture chamber 1, a telescopic sleeve 34 is fixedly arranged at the top of the air pressure bin 33 and close to the positions of four corners, the telescopic sleeve 34 is communicated with the inner cavity of the air pressure bin 33, a telescopic rod 35 is movably sleeved in the telescopic sleeve 34, and the telescopic rod 35 is fixedly connected with the bottom of the bearing device 8.

The feeding device 7 comprises an atomizer 71, a feeding pipe 72 is fixedly arranged at the output end of the atomizer 71, a distributing chamber 73 is fixedly arranged at one end of the feeding pipe 72, two distributing chambers 73 are respectively arranged at the bottom of the left cavity and the bottom of the right cavity of the culture chamber 1 and near the partition plate 4 and are connected through a communicating pipe, distributing pipes 74 are fixedly arranged at the top of the distributing chamber 73, the distributing pipes 74 are distributed in a linear array mode, the distributing pipes 74 are fixedly connected with the partition plate 4, and the distributing pipes 74 are communicated with the inner cavities of the distributing chambers 73.

The distributing pipe 74 corresponds to and communicates with the supply hole i 5.

The bearing device 8 comprises a bottom plate 81, side plates 82 are fixedly arranged on two sides of the top of the bottom plate 81, a supply hole II 83 is formed in a position, close to the top, of one side of the side plate 82, the supply hole II 83 is distributed in a linear array mode, a matching groove 84 is formed in the top of the side plate 82, the matching groove 84 is aligned with the triggering block I6 up and down, a limit chute 85 is fixedly arranged on one side of the side plate 82, a bearing plate 86 is arranged on the top of the limit chute 85, and communication holes 87 are formed in the position, close to the two sides, of the top of the bearing plate 86.

The supply hole II 83 corresponds to the supply hole I5, and the supply hole II 83 and the supply hole I5 are in a staggered state in the initial state.

The trigger device 9 comprises a travel sleeve 91, the travel sleeve 91 is fixedly arranged at the bottom of the inner cavity of the culture chamber 1 and positioned at the two sides of the lifting device 3, a gear chamber 92 is fixedly arranged at one side of the top of the travel sleeve 91, a transmission gear 93 is movably sleeved in the gear chamber 92, a torsion bar 94 is fixedly arranged at one end of the transmission gear 93, please refer to fig. 1-9 and fig. 11-17, the atomizer 71 atomizes culture solution and conveys the culture solution to the inside of the material distributing chamber 73 through a supply pipe 72, the atomized nutrient solution enters the culture dish positioned at the top of the bearing plate 86 through the torsion bar 94, a supply hole I5 and a supply hole II 83, so that the nutrient solution is continuously added to the culture dish, too much nutrient solution does not need to be added in the culture dish in an initial state, the problem that the cell growth and division speed is too fast due to eutrophication in the culture dish is avoided, and thus the speed of cell growth and division is controlled, an experimenter can observe and intervene better in the cell growth and division process, the reliability of the device is improved, the inner portion of the sleeve is provided with a travel bar 95, and a travel bar 95 is fixedly arranged at one side of the travel bar 95, and a travel bar 95 is meshed with the movable travel bar 95 at the top of the support plate.

Example two

On the basis of the first embodiment, referring to fig. 1-9 and fig. 11-17, as the cells in the culture dish grow and divide, the total weight of the culture dish and the cells is continuously increased, so that the carrying device 8 moves in a vertically downward direction under the action of the increase of the total weight, and as the carrying device 8 moves, the feeding hole i 5 and the feeding hole ii 83 approach each other, so that the communication degree between the feeding hole i 5 and the feeding hole ii 83 is improved, so that more atomized nutrient solution enters the interior of the carrying device 8 through the torsion bar 94, the feeding hole i 5 and the feeding hole ii 83, the nutrient solution provided by the device is also continuously improved in the process of ensuring that the cell number in the culture dish is increased, the atomized nutrient solution provided by the atomizer 71 is ensured to be kept at an optimal level, the waste of the nutrient solution is avoided, and the use cost of the device is reduced.

The output end of the trigger block I6 is connected with the input end of the air feeder 31 in a signal connection mode.

The output end of the trigger block II 96 is connected with the input end of the sealing door 2 in a signal connection mode.

Referring to fig. 1-9 and fig. 11-17, as the cells grow and divide, the carrying device 8 moves downward continuously along the vertical direction, so that the bottom of the carrying device 8 approaches the trigger device 9 continuously until the bottom of the carrying device 8 contacts the trigger block ii 96, at this time, the output end of the trigger block ii 96 is connected with the input end of the sealing door 2 in a signal connection manner, so that the sealing door 2 is opened, and the experimenter is reminded that the cell culture is completed, meanwhile, the torsion bar 94 is rotated to drive the transmission gear 93 to rotate, and one side of the travel bar 95 is meshed with the transmission gear 93, so as to control the height of the travel bar 95, and adjust the weight when the carrying device 8 contacts the trigger block ii 96, so that the experimenter can adjust the height of the travel bar 95 according to the requirement, and the practicability of the device is improved.

Referring to fig. 1-17, before use, the air feeder 31 can be used to fill air into the air pressure bin 33, at this time, the telescopic rod 35 stretches out from the interior of the telescopic sleeve 34 under the action of air pressure, so that the bearing device 8 is integrally jacked up until the matching groove 84 contacts with the trigger block i 6, at this time, the output end of the trigger block i 6 is connected with the input end of the air feeder 31 in a signal connection manner, so that the air feeder 31 stops injecting air into the air pressure bin 33 after the bearing device 8 reaches the top, the bearing device 8 can be at the maximum height in an initial state, the internal air pressure of the air pressure bin 33 also reaches the weight of the bearing device 8, the weight control of the device on cells in the culture dish is more accurate, and the practicability of the device is further improved.

Claims

1. An incubator for cell culture, comprising a culture chamber (1): the utility model provides a culture room, including culture room (1), sealed door (2) are installed in front movable mounting of culture room (1), the bottom fixed mounting of culture room (1) inner chamber has lifting device (3), all fixed mounting has division board (4) on the position that culture room (1) inner chamber bottom is close to both sides, division board (4) divide into left chamber, well chamber and right chamber with the inner chamber of culture room (1), feed port I (5) have been seted up to one side of division board (4), fixed mounting has trigger piece I (6) on the position that division board (4) one side is close to the top, fixed mounting has feeding device (7) on the position that the left chamber of culture room (1) is close to division board (4), the top fixed mounting of lifting device (3) has loading attachment (8), fixed mounting has trigger device (9) on the position that culture room (1) inner chamber bottom is located loading attachment (8) both sides.

2. The incubator according to claim 1, wherein the lifting device (3) comprises an air supply machine (31), an air supply pipe (32) is fixedly arranged at the output end of the air supply machine (31), an air pressure bin (33) is fixedly arranged at one end of the air supply pipe (32), a telescopic sleeve (34) is fixedly arranged at the top of the air pressure bin (33) near the positions of four corners, and a telescopic rod (35) is movably sleeved in the telescopic sleeve (34).

3. The incubator for cell culture according to claim 2, wherein the feeding device (7) comprises an atomizer (71), a feeding pipe (72) is fixedly arranged at the output end of the atomizer (71), a distributing chamber (73) is fixedly arranged at one end of the feeding pipe (72), the number of the distributing chambers (73) is two, the distributing chambers are respectively arranged at the positions, close to the partition plate (4), of the bottoms of the left cavity and the right cavity of the incubator (1) and are connected through a communicating pipe, a distributing pipe (74) is fixedly arranged at the top of the distributing chamber (73), and the distributing pipe (74) is communicated with the inner cavity of the distributing chamber (73).

4. A cell culture incubator according to claim 3, wherein the dividing pipe (74) is in communication with the supply port i (5).

5. The incubator according to claim 4, wherein the carrying device (8) comprises a bottom plate (81), side plates (82) are fixedly mounted on two sides of the top of the bottom plate (81), a supply hole II (83) is formed in a position, close to the top, of one side of the side plate (82), a matching groove (84) is formed in the top of the side plate (82), a limit sliding groove (85) is fixedly mounted on one side of the side plate (82), a carrying plate (86) is placed on the top of the limit sliding groove (85), and communication holes (87) are formed in positions, close to the two sides, of the top of the carrying plate (86).

6. The incubator according to claim 5, wherein the supply hole II (83) corresponds to the supply hole I (5), and the supply hole II (83) and the supply hole I (5) are staggered in the initial state.

7. The incubator for cell culture according to claim 6, wherein the trigger device (9) comprises a stroke sleeve (91), the stroke sleeve (91) is fixedly installed at the bottom of the inner cavity of the culture chamber (1) and located at the positions of two sides of the lifting device (3), a gear chamber (92) is fixedly installed on one side of the top of the stroke sleeve (91), a transmission gear (93) is movably sleeved in the gear chamber (92), a torsion bar (94) is fixedly installed at one end of the transmission gear (93), a stroke rod (95) is movably installed in the stroke sleeve (91), one side of the stroke rod (95) is meshed with the transmission gear (93), and a trigger block II (96) is fixedly installed on the top of the stroke rod (95).

8. Incubator according to claim 7, characterized in that the output of the trigger block i (6) is connected to the input of the air supply (31) by means of a signal connection.

9. Incubator for cell culture according to claim 8, characterized in that the output of the trigger block ii (96) is connected to the input of the sealing door (2) by means of a signal connection.