CN114107194A - Immune cell amplification culture method and device - Google Patents
Immune cell amplification culture method and device Download PDFInfo
- Publication number
- CN114107194A CN114107194A CN202111387469.1A CN202111387469A CN114107194A CN 114107194 A CN114107194 A CN 114107194A CN 202111387469 A CN202111387469 A CN 202111387469A CN 114107194 A CN114107194 A CN 114107194A
- Authority
- CN
- China
- Prior art keywords
- culture
- plate
- fixed
- incubator
- cell culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000002865 immune cell Anatomy 0.000 title claims abstract description 23
- 230000003321 amplification Effects 0.000 title claims abstract description 11
- 238000012136 culture method Methods 0.000 title claims abstract description 11
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 11
- 238000004113 cell culture Methods 0.000 claims abstract description 49
- 238000000034 method Methods 0.000 claims abstract description 12
- 210000004027 cell Anatomy 0.000 claims description 7
- 230000000149 penetrating effect Effects 0.000 claims description 6
- 230000010261 cell growth Effects 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 3
- 210000005259 peripheral blood Anatomy 0.000 claims description 3
- 239000011886 peripheral blood Substances 0.000 claims description 3
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 claims description 3
- 229920006395 saturated elastomer Polymers 0.000 claims description 3
- 238000007789 sealing Methods 0.000 abstract description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 206010063659 Aversion Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003028 elevating effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
Abstract
The invention relates to the technical field of cell culture auxiliary devices, in particular to an immune cell amplification culture method and device, wherein when cell culture is carried out, a sealing door of a culture instrument cannot move a cell culture plate in the opening and closing process and the process that the culture plate enters and exits the culture instrument, so that the culture solution in each hole in the culture plate is prevented from splashing outwards; the method comprises the following steps: the cell culture plate comprises a culture box, a hinge, a box door, a first long hole, a second long hole, a slide block and a cell culture plate, wherein the culture box is hollow and is provided with an opening at one side, the box door is rotatably arranged at the opening through the hinge, two bases are fixed in the culture box, each base is fixedly provided with a telescopic slide rail, the telescopic ends of the two telescopic slide rails are fixedly provided with a movable plate, the movable plate is provided with two long holes I in a circumferential array by taking the central line of the movable plate as an axis, the first long hole is provided with one slide block in a sliding manner, and the two slide blocks synchronously and reversely move for clamping and releasing the cell culture plate; the moving plate is rotatably provided with a central shaft, the central shaft is coaxial with the central line of the moving plate, and a first gear is fixed on the central shaft.
Description
Technical Field
The invention relates to the technical field of cell culture auxiliary devices, in particular to an immune cell amplification culture method and device.
Background
Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology.
When carrying out the amplification culture to immune cell, need select separately immune cell, then place in porous cell culture board, cultivate with 37 ℃, 5% carbon dioxide's condition in the culture apparatus, wherein the sealing door of culture apparatus is opening and close the in-process and the culture plate is passed in and out the culture apparatus in-process and probably produces the aversion, and the culture solution in each hole in the culture plate is probably outwards spill, and immune cell is mostly suspension growth, spills along with the culture solution easily, influences the culture effect.
Disclosure of Invention
In order to solve the technical problems, the invention provides an immune cell amplification culture method and device, wherein when cell culture is carried out, a sealing door of a culture instrument cannot move a cell culture plate in the opening and closing process and the process that the culture plate enters and exits the culture instrument, so that the culture solution in each hole in the culture plate is prevented from being splashed outwards.
The invention relates to an immune cell amplification culture device, which comprises: the cell culture plate comprises a culture box, a hinge, a box door, a first long hole, a second long hole, a slide block and a cell culture plate, wherein the culture box is hollow and is provided with an opening at one side, the box door is rotatably arranged at the opening through the hinge, two bases are fixed in the culture box, each base is fixedly provided with a telescopic slide rail, the telescopic ends of the two telescopic slide rails are fixedly provided with a movable plate, the movable plate is provided with two long holes I in a circumferential array by taking the central line of the movable plate as an axis, the first long hole is provided with one slide block in a sliding manner, and the two slide blocks synchronously and reversely move for clamping and releasing the cell culture plate;
a central shaft is rotatably mounted on the moving plate, the central shaft is coaxial with the central line of the moving plate, a first gear is fixed on the central shaft, two strip holes II which are not concentric with the first gear are circumferentially arranged on the first gear, a first driving shaft is fixed on each sliding block, and the first driving shaft extends into the strip holes II;
a hollow shaft is rotatably mounted on the movable plate, a gear II meshed with the gear I is fixed on the hollow shaft, a through hole I is formed in the hollow shaft, the through hole I sequentially comprises a spiral section and a vertical section I from top to bottom, a fixed plate is fixed on one side inside the incubator, a through hole II is formed in the fixed plate, and the through hole II sequentially comprises a vertical section II and an inclined section from top to bottom from outside to inside;
and a lifting mechanism is fixed on the moving plate, a lifting block is installed at the output end of the lifting mechanism, a second driving shaft penetrating through the spiral section is fixed at one end of the lifting block, and a third driving shaft penetrating through the vertical section II is fixed at the other end of the lifting block.
Further, elevating system includes the door type board with movable plate fixed connection, rotates on the door type board and installs the lead screw, elevator and lead screw threaded connection are fixed with two guide bars on the door type board, and two guide bars all pass the elevator, install on the tip position of lead screw and be used for driving its rotatory motor.
Furthermore, a bearing is fixed on each driving shaft I, and the outer ring of the bearing is tangent to the inner wall of the elongated hole II.
Furthermore, a proximity switch for controlling the motor is arranged in the incubator.
Furthermore, cushions are arranged on one sides of the two sliding blocks close to the central line of the moving plate.
Further, the height of the bottom end of the vertical section II is lower than that of the bottom end of the spiral section.
Furthermore, a support leg is installed at each of four corners of the bottom of the incubator.
The immune cell amplification culture method comprises the following steps:
s1, extracting peripheral blood mononuclear cells from human peripheral blood;
s2, inoculating the cells extracted in the step S1 into a culture bottle containing a culture medium for culture;
s3, introducing the cells in the culture bottle obtained in the step S2 into a culture bag;
and S4, obtaining the immune cells.
Further, step SThe culture method comprises the following steps: placing the culture flask on the moving plate of claims 1-7, and moving the moving plate to the inside of the culture box of claims 1-7, at 37 deg.C, saturated humidity, 5% CO2Culturing in incubator for 3-5 days.
Compared with the prior art, the invention has the beneficial effects that: before the cell culture board enters into the incubator inside, this device can press from both sides tight with cell culture board or other cell culture device earlier, then remove cell culture board or other cell culture device, then close the chamber door again, when taking out cell culture board or other cell culture device, open chamber door and cell culture board or other cell culture device and shift out the incubator in-process, two sliders press from both sides tight cell culture board or other cell culture device all the time, prevent that the culture solution from spilling.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a top right front perspective view of FIG. 1;
FIG. 3 is a top perspective view of the left front side hidden incubator of FIG. 1 and behind the door of the incubator;
FIG. 4 is an exploded view of FIG. 3;
FIG. 5 is a block diagram of the hollow shaft, the helical section, and the vertical section one;
in the drawings, the reference numbers: 1. an incubator; 2. a box door; 3. a base; 4. a telescopic slide rail; 5. moving the plate; 6. a first long hole; 7. a slider; 8. a central shaft; 9. a first gear; 10. a second elongated hole; 11. a first driving shaft; 12. a hollow shaft; 13. a second gear; 14. a helical section; 15. a first vertical section; 16. a fixing plate; 17. a second vertical section; 18. an inclined section; 19. a lifting block; 20. a second driving shaft; 21. a third driving shaft; 22. a screw rod; 23. a guide bar; 24. a motor; 25. a bearing; 26. a proximity switch; 27. a soft cushion.
Detailed Description
The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted" and "connected" are to be interpreted broadly, e.g., as being either fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
As shown in fig. 1 to 5, the immune cell expansion culture method and device of the present invention comprises: the cell culture plate comprises an incubator 1, a hinge, a box door 2, a movable plate 5, two strip holes 6, a slide block 7 and two slide blocks 7, wherein the incubator 1 is hollow and is provided with an opening at one side, the box door 2 is rotatably arranged at the opening through the hinge, the two bases 3 are fixed inside the incubator 1, each base 3 is fixed with one telescopic slide rail 4, the movable plate 5 is fixed on the telescopic ends of the two telescopic slide rails 4, the two strip holes 6 are circumferentially arranged on the movable plate 5 by taking the central line of the movable plate 5 as an axis, the slide block 7 is slidably arranged at the position of each strip hole 6, and the two slide blocks 7 synchronously and reversely move for clamping and releasing the cell culture plate;
a central shaft 8 is rotatably mounted on the moving plate 5, the central shaft 8 is coaxial with the central line of the moving plate 5, a first gear 9 is fixed on the central shaft 8, two long holes 10 which are not concentric with the first gear 9 are circumferentially arranged on the first gear 9, a first driving shaft 11 is fixed on each sliding block 7, and the first driving shafts 11 extend into the long holes 10;
a hollow shaft 12 is rotatably mounted on the moving plate 5, a gear II 13 meshed with the gear I9 is fixed on the hollow shaft 12, a through hole I is formed in the hollow shaft 12, the through hole I sequentially comprises a spiral section 14 and a vertical section 15 from top to bottom, a fixing plate 16 is fixed on one side inside the incubator 1, a through hole II is formed in the fixing plate 16, and the through hole II sequentially comprises a vertical section II 17 and an inclined section 18 from top to bottom from outside to inside;
a lifting mechanism is fixed on the moving plate 5, a lifting block 19 is installed at the output end of the lifting mechanism, a second driving shaft 20 penetrating through the spiral section 14 is fixed at one end of the lifting block 19, and a third driving shaft 21 penetrating through the vertical section 17 is fixed at the other end of the lifting block;
in the present embodiment, as shown in the figure, the moving plate 5 is at the outermost position relative to the incubator 1, when in use, the cell culture plate is placed between the two sliding blocks 7, then the lifting mechanism is operated to simultaneously move the lifting block 19, the second driving shaft 20 and the third driving shaft 21 downwards, when the second driving shaft 20 passes through the spiral section 14 of the hollow shaft 12, the third driving shaft 21 moves downwards along the second vertical section 17 of the fixed plate 16, and the hollow shaft 12 and the second gear 13 rotate, because the first gear 9 is engaged with the second gear 13, the first gear 9 and the central shaft 8 rotate, and because the first driving shaft 11 fixedly connected with the sliding blocks 7 extends into the second elongated hole 10, the rotating first gear 9 enables the two sliding blocks 7 to move towards each other to clamp the cell culture plate, and then the second driving shaft 20 moves to the first vertical section 15, the second driving shaft 20 which moves downwards continuously does not affect the hollow shaft 12, the third driving shaft 21 moves to the inclined section 18 of the fixed plate 16, and the fixed plate 16 is fixed, so that the third driving shaft 21 moves the moving plate 5, the cell culture plate, the lifting mechanism and the like to the inside of the incubator 1, after the moving plate, the cell culture plate, the lifting mechanism and the chamber door 2 are closed after the moving plate completely enters the inside of the incubator 1, in conclusion, before the cell culture plate enters the inside of the incubator 1, the device can clamp the cell culture plate or other cell culture devices, then move the cell culture plate or other cell culture devices, then close the chamber door 2, and when the cell culture plate or other cell culture devices are taken out, the chamber door 2 and the cell culture plate or other cell culture devices are opened and moved out of the incubator 1, the two sliding blocks 7 always clamp the cell culture plate or other cell culture devices to prevent the culture solution from spilling.
As a preferable scheme of the above embodiment, the lifting mechanism includes a door-shaped plate fixedly connected with the moving plate 5, a screw rod 22 is rotatably mounted on the door-shaped plate, the lifting block 19 is in threaded connection with the screw rod 22, two guide rods 23 are fixed on the door-shaped plate, both the two guide rods 23 pass through the lifting block 19, and a motor 24 for driving the screw rod 22 to rotate is mounted at an end position of the screw rod;
in the embodiment, the motor 24 is powered on to drive the screw rod 22 to rotate, because the screw rod 22 is in threaded connection with the lifting block 19, and the lifting block 19 cannot rotate under the action of the two guide rods 23, the lifting block 19 can be changed in height by rotating the screw rod 22, and the motor 24 has a self-locking function after being stopped by adopting the driving mode of the screw rod 22.
As a preferable scheme of the above embodiment, each driving shaft I11 is fixed with a bearing 25, and the outer ring of the bearing 25 is tangent to the inner wall of the elongated hole II 10;
in the embodiment, the bearing 25 is arranged on the driving shaft and is used for avoiding the driving shaft from contacting with the inner wall of the second elongated hole 10, so that the driving shaft can be prevented from being abraded, and the moving synchronism of the two sliding blocks 7 can be ensured.
As a preferable scheme of the above embodiment, a proximity switch 26 for controlling the motor 24 is installed inside the incubator 1;
in this embodiment, when the door-shaped plate moves into the incubator 1 to trigger the proximity switch 26, the proximity switch 26 controls the motor 24 to turn off, thereby preventing the moving plate 5 from colliding with the incubator 1 without manually controlling the motor 24.
As a preferable scheme of the above embodiment, soft pads 27 are mounted on one sides of the two sliders 7 close to the central line of the moving plate 5;
in this embodiment, the soft pad 27 on the slide 7 directly contacts with the cell culture device, the soft pad 27 deforms during the clamping process, the applicability is improved, and the soft pad 27 does not damage the cell culture device.
As a preferable scheme of the above embodiment, the height of the bottom end of the second vertical section 17 is lower than that of the bottom end of the spiral section 14;
in this embodiment, the moving plate 5 is moved into the incubator 1 after the cell culture apparatus is clamped.
As the preferable scheme of the above embodiment, four corners of the bottom of the incubator 1 are respectively provided with a support leg;
in this embodiment, the incubator 1 is supported by the legs.
The immune cell amplification culture method comprises the following steps:
s1, extracting peripheral blood mononuclear cells from human peripheral blood;
s2, inoculating the cells extracted in the step S1 into a culture bottle containing a culture medium for culture;
s3, introducing the cells in the culture bottle obtained in the step S2 into a culture bag;
and S4, obtaining the immune cells.
As a preferable embodiment of the above embodiment, the culture method in step S2 is: the culture flask is placed on the moving plate 5 of claims 1 to 7, and the moving plate 5 is moved to the inside of the culture box 1 of claims 1 to 7, and cultured in the culture box 1 at 37 ℃ and saturated humidity and 5% CO2 for 3 to 5 days.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (9)
1. An immune cell expansion culture device, comprising: the cell culture plate clamping device comprises an incubator (1), wherein the incubator is hollow, one side of the incubator is provided with an opening, a box door (2) is rotatably arranged at the opening through a hinge, two bases (3) are fixed inside the incubator (1), each base (3) is fixedly provided with a telescopic slide rail (4), a movable plate (5) is fixed at the telescopic end of each telescopic slide rail (4), two strip holes I (6) are circumferentially arranged on the movable plate (5) in a circumferential array by taking the central line of the movable plate (5) as an axis, a sliding block (7) is slidably arranged at each strip hole I (6), and the two sliding blocks (7) synchronously and reversely move to clamp and release the cell culture plate;
a central shaft (8) is rotatably mounted on the movable plate (5), the central shaft (8) is coaxial with the central line of the movable plate (5), a first gear (9) is fixed on the central shaft (8), two long holes (10) which are not concentric with the first gear (9) are formed in the first gear (9) in a circumferential array mode, a first driving shaft (11) is fixed on each sliding block (7), and the first driving shaft (11) extends into the long holes (10);
a hollow shaft (12) is rotatably mounted on the moving plate (5), a gear II (13) meshed with the gear I (9) is fixed on the hollow shaft (12), a through hole I is formed in the hollow shaft (12), the through hole I is sequentially a spiral section (14) and a vertical section I (15) from top to bottom, a fixing plate (16) is fixed on one side inside the incubator (1), a through hole II is formed in the fixing plate (16), and the through hole II is sequentially a vertical section II (17) and an inclined section (18) from top to bottom from inside;
a lifting mechanism is fixed on the moving plate (5), a lifting block (19) is installed at the output end of the lifting mechanism, a second driving shaft (20) penetrating through the spiral section (14) is fixed at one end of the lifting block (19), and a third driving shaft (21) penetrating through the vertical section (17) is fixed at the other end of the lifting block.
2. The immune cell amplification culture device according to claim 1, wherein the lifting mechanism comprises a door-shaped plate fixedly connected with the moving plate (5), a screw rod (22) is rotatably mounted on the door-shaped plate, the lifting block (19) is in threaded connection with the screw rod (22), two guide rods (23) are fixed on the door-shaped plate, the two guide rods (23) both penetrate through the lifting block (19), and a motor (24) for driving the screw rod (22) to rotate is mounted at an end position of the screw rod.
3. An immune cell expansion and culture device according to claim 2, wherein each driving shaft I (11) is fixed with a bearing (25), and the outer ring of the bearing (25) is tangent with the inner wall of the elongated hole II (10).
4. An apparatus for the amplification and culture of immune cells according to claim 3, characterized in that the incubator (1) is internally provided with a proximity switch (26) for controlling the motor (24).
5. An apparatus for amplifying and culturing immune cells according to claim 4, wherein the two sliders (7) are provided with a soft pad (27) on one side near the center line of the moving plate (5).
6. An apparatus as claimed in claim 5, wherein the height of the bottom end of the second vertical section (17) is lower than that of the bottom end of the spiral section (14).
7. An apparatus for the expansion and culture of immune cells according to claim 6, wherein the bottom of the incubator (1) is provided with a foot at each of the four corners.
8. An immune cell expansion culture method is characterized by comprising the following steps:
s1, extracting peripheral blood mononuclear cells from human peripheral blood;
s2, inoculating the cells extracted in the step S1 into a culture bottle containing a culture medium for culture;
s3, introducing the cells in the culture bottle obtained in the step S2 into a culture bag;
and S4, obtaining the immune cells.
9. The method for culturing and expanding immune cells according to claim 8, wherein the culturing method of step S2 is: the culture flask was placed on the moving plate (5) as described in claims 1 to 7, and the moving plate (5) was moved to the inside of the incubator (1) as described in claims 1 to 7, and cultured in the incubator (1) at 37 ℃ with saturated humidity and 5% CO2 for 3 to 5 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111387469.1A CN114107194B (en) | 2021-11-22 | 2021-11-22 | Immune cell expansion culture method and device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111387469.1A CN114107194B (en) | 2021-11-22 | 2021-11-22 | Immune cell expansion culture method and device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114107194A true CN114107194A (en) | 2022-03-01 |
| CN114107194B CN114107194B (en) | 2024-01-30 |
Family
ID=80439207
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111387469.1A Active CN114107194B (en) | 2021-11-22 | 2021-11-22 | Immune cell expansion culture method and device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114107194B (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014171389A (en) * | 2013-03-05 | 2014-09-22 | Shimadzu Corp | Cell culture device |
| CN109207424A (en) * | 2018-10-24 | 2019-01-15 | 河南省肿瘤医院 | A kind of cultural method of immunocyte |
| CN210736784U (en) * | 2019-07-30 | 2020-06-12 | 南京平港生物技术有限公司 | Cell culture box |
| CN111321072A (en) * | 2020-03-05 | 2020-06-23 | 通用生物系统（安徽）有限公司 | Cell culture box for preparing monoclonal antibody |
| CN212199289U (en) * | 2019-11-13 | 2020-12-22 | 北京易科拜德科技有限公司 | Cell culture device |
| CN212925056U (en) * | 2020-07-17 | 2021-04-09 | 威海见生生物技术有限公司 | Immune cell culture device |
| CN214004663U (en) * | 2020-11-12 | 2021-08-20 | 南昌龙来福生物科技有限公司 | Biological cell constant temperature incubator |
| CN214060537U (en) * | 2020-12-04 | 2021-08-27 | 冬青南京生物科技有限公司 | Immune cell amplification culture instrument |
-
2021
- 2021-11-22 CN CN202111387469.1A patent/CN114107194B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014171389A (en) * | 2013-03-05 | 2014-09-22 | Shimadzu Corp | Cell culture device |
| CN109207424A (en) * | 2018-10-24 | 2019-01-15 | 河南省肿瘤医院 | A kind of cultural method of immunocyte |
| CN210736784U (en) * | 2019-07-30 | 2020-06-12 | 南京平港生物技术有限公司 | Cell culture box |
| CN212199289U (en) * | 2019-11-13 | 2020-12-22 | 北京易科拜德科技有限公司 | Cell culture device |
| CN111321072A (en) * | 2020-03-05 | 2020-06-23 | 通用生物系统（安徽）有限公司 | Cell culture box for preparing monoclonal antibody |
| CN212925056U (en) * | 2020-07-17 | 2021-04-09 | 威海见生生物技术有限公司 | Immune cell culture device |
| CN214004663U (en) * | 2020-11-12 | 2021-08-20 | 南昌龙来福生物科技有限公司 | Biological cell constant temperature incubator |
| CN214060537U (en) * | 2020-12-04 | 2021-08-27 | 冬青南京生物科技有限公司 | Immune cell amplification culture instrument |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114107194B (en) | 2024-01-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN114107194A (en) | Immune cell amplification culture method and device | |
| KR20100011175A (en) | Apparatus for cells culture and control method thereof | |
| CN113973712B (en) | Plant tissue culture device | |
| CN208038419U (en) | A kind of drawing and pulling type multilayer shaken cultivation case | |
| CN210711526U (en) | Carbon dioxide incubator convenient for taking and placing culture dish | |
| CN214937404U (en) | A culture apparatus for microbiological detection | |
| CN111394241A (en) | Bacterial organism culture cabinet | |
| CN220618887U (en) | Stem cell chemical examination detects reaction unit | |
| CN220520465U (en) | Biological test sample culture apparatus | |
| CN213780053U (en) | Constant temperature incubator that food safety inspection used | |
| CN219861290U (en) | Incubator convenient to place culture dish | |
| CN213596303U (en) | Cell culture bottle constant temperature platform | |
| CN220750474U (en) | Biological product storage equipment | |
| CN215162684U (en) | Device for rapidly screening strains in streptomyces without water absorption | |
| CN213570531U (en) | Bioengineering cell culture device | |
| CN220116556U (en) | Portable low-temperature plasma mutagenesis device | |
| CN220745964U (en) | Amplifying device for stem cells | |
| CN219490037U (en) | Cell culture apparatus | |
| CN218710559U (en) | Bacteriostatic device | |
| CN219977935U (en) | Device for fixing ESD operation specimen | |
| CN213113360U (en) | Stem cell culture device | |
| CN114540193A (en) | Breast cancer cell and T cell co-culture method for improving tumor killing capability of immune cells | |
| CN214553730U (en) | Adjustable test bed for biological research and development | |
| CN219861347U (en) | Microorganism detection device | |
| CN214654817U (en) | Clinical laboratory's bacterial microorganism sample preparation platform |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |