CN214060537U

CN214060537U - Immune cell amplification culture instrument

Info

Publication number: CN214060537U
Application number: CN202022890468.6U
Authority: CN
Inventors: 王志梁
Original assignee: Dongqing Nanjing Bio Tech Co ltd
Current assignee: Dongqing Nanjing Bio Tech Co ltd
Priority date: 2020-12-04
Filing date: 2020-12-04
Publication date: 2021-08-27
Anticipated expiration: 2030-12-04

Abstract

The utility model discloses an immune cell amplification culture instrument, including main part mechanism, main part mechanism has seted up the cultivation room including cultivateing the appearance main part, cultivates the inside of appearance main part, and the one end surface of cultivateing the appearance main part is rotated and is connected with sealing door, and the inside of cultivateing the room is provided with buffer gear, and buffer gear's upper surface is provided with fixture, the utility model discloses the beneficial effect who reaches is: prevent through setting up horizontal buffer board and vertical buffer board that the culture solution from spilling in the air vent of cultivateing the board, influence the final data of cultivateing, wherein when opening and close the sealing door, probably can make the appearance main part of cultivateing take place to shift, make inside cultivation board take place to vibrate, when taking place to vibrate, the slider of vertical buffer board bottom slides along the stock, and compress the spring at both ends, carry out vertical buffering, horizontal buffer board drives branch and removes, branch drives the pulley through the pivot and undulates in the arc wall and slides, carry out horizontal buffering, prevent the culture solution spill.

Description

Immune cell amplification culture instrument

Technical Field

The utility model relates to an amplification culture instrument, in particular to an immune cell amplification culture instrument, which belongs to the technical field of cell culture.

Background

Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology.

When the immune cells are subjected to amplification culture, the immune cells need to be sorted and then placed in a porous cell culture plate, culture is performed in a culture instrument under the conditions of 37 ℃ and 5% carbon dioxide, wherein a sealing door of the culture instrument can shift in the opening and closing processes, the culture solution in each hole in the culture plate can be splashed outwards, most of the immune cells grow in a suspension manner, and the immune cells are easy to spill out along with the culture solution, so that the culture effect is influenced.

SUMMERY OF THE UTILITY MODEL

The utility model provides an immune cell amplification culture instrument, the effectual problem that exists of having solved among the prior art.

In order to solve the technical problem, the utility model provides a following technical scheme:

the utility model relates to an immune cell amplification culture instrument, including main part mechanism, main part mechanism is including cultivateing the appearance main part, the inside of cultivateing the appearance main part has been seted up and has been cultivateed the room, the one end surface rotation of cultivateing the appearance main part is connected with sealing door, the inside of cultivateing the room is provided with buffer gear, buffer gear's upper surface is provided with fixture.

As a preferred technical proposal of the utility model, the buffer mechanism comprises two elongated slots which are both arranged on the surface of the inner bottom end of the culture chamber, the bottom surface of the interior of the culture chamber is connected with a longitudinal buffer plate in a sliding way, the bottom surface of the longitudinal buffer plate is fixedly connected with four sliding blocks, the side surfaces of the four sliding blocks are respectively provided with a sliding hole, the inner wall surfaces of two ends of the two long grooves are respectively fixedly connected with a long rod, two ends of the two long rods are respectively sleeved with a spring, the upper surface of the longitudinal buffer plate is provided with a transverse buffer plate, the lower surface of the transverse buffer plate is fixedly connected with four support rods, the bottom ends of the four support rods are fixedly connected with rotating shafts, the outer surfaces of the four rotating shafts are rotatably connected with pulleys, four through grooves are formed in the upper surface of the longitudinal buffer plate, and four arc-shaped grooves are formed in the longitudinal buffer plate.

As a preferred technical scheme of the utility model, fixture includes two fixed plates, two fixed plate difference fixed connection is at the upper surface both ends of horizontal buffer board, two the thread groove has all been seted up to the side surface of fixed plate, two the equal threaded connection in inside of thread groove has the threaded rod, two the one end of threaded rod is all rotated and is connected with splint, two the equal fixedly connected with of one end that splint were kept away from to the threaded rod changes the piece.

As an optimal technical scheme of the utility model, per two slider and same elongated slot sliding connection, per two the slide opening and same stock sliding connection.

As an optimal technical solution of the present invention, four the two ends of the spring are respectively in contact with the side surface of the slider and the end wall surface of the elongated slot.

As an optimal technical scheme of the utility model, four the arc wall is rather than the pulley sliding connection that corresponds, four respectively lead to the groove all with the branch sliding connection that corresponds.

The utility model discloses the beneficial effect who reaches is: prevent through setting up horizontal buffer board and vertical buffer board that the culture solution from spilling in the air vent of cultivateing the board, influence the final data of cultivateing, wherein when opening and close the sealing door, probably can make the appearance main part of cultivateing take place to shift, make inside cultivation board take place to vibrate, when taking place to vibrate, the slider of vertical buffer board bottom slides along the stock, and compress the spring at both ends, carry out vertical buffering, horizontal buffer board drives branch and removes, branch drives the pulley through the pivot and undulates in the arc wall and slides, carry out horizontal buffering, prevent the culture solution spill.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention. In the drawings:

fig. 1 is a side view of the internal structure of the present invention;

FIG. 2 is a front view of the internal structure of the present invention;

FIG. 3 is an enlarged view of a portion A of FIG. 1;

FIG. 4 is an enlarged view of the portion B of FIG. 2;

fig. 5 is a schematic view of the three-dimensional structure of the vertical plate of the present invention.

In the figure: 1. a main body mechanism; 11. a culture apparatus main body; 12. a culture chamber; 13. a sealing door; 2. a buffer mechanism; 21. a long groove; 22. a long rod; 23. a spring; 24. a longitudinal buffer plate; 25. a slider; 26. a slide hole; 27. a lateral buffer plate; 28. an arc-shaped slot; 29. a strut; 210. a rotating shaft; 211. a pulley; 212. a through groove; 3. a clamping mechanism; 31. a fixing plate; 32. a thread groove; 33. a threaded rod; 34. a splint; 35. and (6) turning the block.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Example (b): as shown in fig. 1-5, the utility model relates to an immune cell amplification culture instrument, including main body mechanism 1, main body mechanism 1 includes culture instrument main part 11, and culture room 12 has been seted up to the inside of culture instrument main part 11, and the one end surface of culture instrument main part 11 rotates and is connected with sealing door 13, and the inside of culture room 12 is provided with buffer gear 2, and buffer gear 2's upper surface is provided with fixture 3.

The buffer mechanism 2 comprises two elongated slots 21, wherein the two elongated slots 21 are all arranged on the inner bottom end surface of the culture chamber 12, the inner bottom end surface of the culture chamber 12 is connected with a longitudinal buffer plate 24 in a sliding way, the bottom end surface of the longitudinal buffer plate 24 is fixedly connected with four slide blocks 25, the side surfaces of the four slide blocks 25 are all provided with slide holes 26, the inner wall surfaces of the two ends of the two elongated slots 21 are all fixedly connected with long rods 22, the two ends of the two long rods 22 are all sleeved with springs 23, the upper surface of the longitudinal buffer plate 24 is provided with a transverse buffer plate 27, the lower surface of the transverse buffer plate 27 is fixedly connected with four support rods 29, the bottom ends of the four support rods 29 are all fixedly connected with rotating shafts 210, the outer surfaces of the four rotating shafts 210 are all rotatably connected with pulleys 211, the upper surface of the longitudinal buffer plate 24 is provided with four through grooves 212, the interior of the longitudinal buffer plate 24 is provided with four arc-shaped grooves 28, and the buffer mechanism 2 performs horizontal and longitudinal buffering, the process of opening and closing the sealing door 13 of the main body 11 of the incubator is buffered to prevent oscillation.

Fixture 3 includes two fixed plates 31, and two fixed plates 31 are fixed connection respectively at the upper surface both ends of horizontal buffer board 27, and thread groove 32 has all been seted up to the side surface of two fixed plates 31, and the equal threaded connection in inside of two thread grooves 32 has threaded rod 33, and the one end of two threaded rods 33 is all rotated and is connected with splint 34, and the equal fixedly connected with of one end that splint 34 were kept away from to two threaded rods 33 changes piece 35, and fixture 3 is used for the fixed cultivation board.

Every two sliding blocks 25 are connected with the same long groove 21 in a sliding mode, every two sliding holes 26 are connected with the same long rod 22 in a sliding mode, and therefore the longitudinal buffer plate 24 can move conveniently.

The two ends of the four springs 23 are respectively arranged in contact with the side surfaces of the slider 25 and the end wall surfaces of the elongated slot 21, and the springs 23 serve as longitudinal buffers.

The four arc-shaped grooves 28 are respectively connected with the corresponding pulleys 211 in a sliding manner, and the four through grooves 212 are respectively connected with the corresponding supporting rods 29 in a sliding manner, so that the transverse buffer plate 27 can be conveniently buffered transversely.

Specifically, when the utility model is used, when the immune cell amplification culture is carried out, firstly, the treated culture solution is placed in each hole of the cell culture plate, the cover is covered, the culture plate is placed on the upper surface of the transverse buffer plate 27 in the culture chamber 12, the culture plate is fixed by using the clamping mechanism 3, the sealing door 13 is closed, the air temperature and the carbon dioxide concentration of the main body 11 of the culture instrument are set, the culture is carried out, wherein, when the sealing door 13 is opened and closed, the main body 11 of the culture instrument can be shifted, the culture plate inside is vibrated, when the vibration is carried out, the slide block 25 at the bottom of the longitudinal buffer plate 24 slides along the long rod 22, the springs 23 at the two ends are compressed, the longitudinal buffer is carried out, the transverse buffer plate 27 drives the supporting rod 29 to move, the supporting rod 29 drives the pulley 211 to slide in the arc-shaped groove 28 through the rotating shaft 210 in a fluctuating way, the transverse buffer is carried out, the culture solution is prevented from splashing, the transverse buffer plate 27 and the longitudinal buffer plate 24 are arranged to prevent the culture solution from splashing out of the ventilation holes of the culture plate, thereby influencing the final data of the culture.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides an immune cell amplification culture instrument, includes main part mechanism (1), its characterized in that, main part mechanism (1) is including cultivateing appearance main part (11), cultivate room (12) have been seted up to the inside of appearance main part (11), the one end surface rotation of cultivateing appearance main part (11) is connected with sealing door (13), the inside of cultivateing room (12) is provided with buffer gear (2), the upper surface of buffer gear (2) is provided with fixture (3).

2. The immune cell amplification culture apparatus according to claim 1, wherein the buffer mechanism (2) comprises two elongated slots (21), the two elongated slots (21) are all opened on the inner bottom surface of the culture chamber (12), the inner bottom surface of the culture chamber (12) is connected with a longitudinal buffer plate (24) in a sliding manner, the bottom surface of the longitudinal buffer plate (24) is fixedly connected with four sliding blocks (25), the side surfaces of the four sliding blocks (25) are all provided with sliding holes (26), the inner wall surfaces of the two ends of the two elongated slots (21) are all fixedly connected with long rods (22), the two ends of the two long rods (22) are all sleeved with springs (23), the upper surface of the longitudinal buffer plate (24) is provided with a transverse buffer plate (27), and the lower surface of the transverse buffer plate (27) is fixedly connected with four support rods (29), four the equal fixedly connected with pivot (210) in bottom of branch (29), four the surface of pivot (210) all rotates and is connected with pulley (211), four logical groove (212) have been seted up to the upper surface of vertical buffer board (24), four arc wall (28) have been seted up to the inside of vertical buffer board (24).

3. The immune cell amplification culture instrument as claimed in claim 1, wherein the clamping mechanism (3) comprises two fixing plates (31), the two fixing plates (31) are respectively and fixedly connected to two ends of the upper surface of the transverse buffer plate (27), thread grooves (32) are respectively formed in the side surfaces of the two fixing plates (31), threaded rods (33) are respectively and fixedly connected to the inner parts of the thread grooves (32), one ends of the two threaded rods (33) are respectively and rotatably connected with a clamping plate (34), and one ends of the two threaded rods (33) far away from the clamping plate (34) are respectively and fixedly connected with a rotating block (35).

4. The apparatus according to claim 2, wherein each two of the sliding blocks (25) are slidably connected to the same elongated slot (21), and each two of the sliding holes (26) are slidably connected to the same elongated rod (22).

5. The apparatus according to claim 2, wherein the four springs (23) are disposed at both ends thereof in contact with the side surfaces of the slider (25) and the end wall surfaces of the elongated slot (21), respectively.

6. The apparatus according to claim 2, wherein four of the arc-shaped slots (28) are slidably connected to the corresponding pulleys (211), and four of the through slots (212) are slidably connected to the corresponding support rods (29).