CN109401963A - A kind of concussion frame of stem cell culture - Google Patents
A kind of concussion frame of stem cell culture Download PDFInfo
- Publication number
- CN109401963A CN109401963A CN201811247531.5A CN201811247531A CN109401963A CN 109401963 A CN109401963 A CN 109401963A CN 201811247531 A CN201811247531 A CN 201811247531A CN 109401963 A CN109401963 A CN 109401963A
- Authority
- CN
- China
- Prior art keywords
- fixed
- incubator
- putting
- board
- support plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
Abstract
The present invention provides a kind of concussion frame of stem cell culture, including incubator, sliding rail, board for putting, blocking mechanism, support plate, spring, bottom plate, vibrating motor, motor and shaft, the incubator is equipped with dodge gate, several spaced board for putting up and down are equipped in the incubator, the sliding rail on the inner wall corresponding with the incubator is fixed on of the both ends of the board for putting is slidably connected, the blocking mechanism is fixed on the board for putting, the board for putting is equipped with several equally distributed placing grooves, the incubator, the support plate, several springs and the bottom plate are fixedly connected sequentially from top to bottom, the incubator is fixed in the support plate, the both ends of the spring are fixedly connected with the support plate and the bottom plate respectively, vibrating motor is fixedly connected with support plate, motor is connect with shaft, shaft Top is fixed at bottom plate bottom center.The present invention has the advantages of preventing culture solution from precipitating, improving the uniformity of culture solution.
Description
Technical field
The present invention relates to a kind of concussion framves of stem cell culture.
Background technique
Stem cell is a kind of multipotential cell with the of self-replication capacity.Under certain condition, it can be divided into more
Kind functioning cell.Stem cell can split into a variety of specializations via mitosis and differentiation there are in all multicellular tissues
Cell, and can use self-renewing to provide more stem cells.All stem cell is carried out using culture solution in the prior art
Culture, stem cell medium is required specific culture environment in the incubation for act on stem cell, to reach stem cell
Adherent or value-added effect, traditional stem cell medium in process of production, stands is easy to appear precipitating for a long time, react not
Phenomena such as uniform, influences the culture effect to stem cell.
Therefore, being badly in need of one kind prevents culture solution from precipitating, and improves the shake of the stem cell culture of the uniformity of culture solution
Swing frame.
Summary of the invention
It is easy to appear precipitating in order to solve the problems, such as that existing stem cell medium is stood for a long time, the purpose of the present invention is mention
For a kind of concussion frame of stem cell culture, there is the advantages of preventing culture solution from precipitating, improving the uniformity of culture solution.
The present invention provides the following technical solutions:
A kind of concussion frame of stem cell culture, including incubator, sliding rail, board for putting, blocking mechanism, support plate, spring,
Bottom plate, vibrating motor, motor and shaft, the incubator are equipped with dodge gate, be equipped in the incubator it is several between the upper and lower every
The board for putting of setting, the sliding rail on the inner wall corresponding with the incubator is fixed on of the both ends of the board for putting, which slides, to be connected
It connects, the blocking mechanism is fixed on the board for putting, and the board for putting is equipped with several equally distributed placing grooves, the training
It supports case, the support plate, several springs and the bottom plate to be fixedly connected sequentially from top to bottom, the incubator is fixed on institute
It states in support plate, the both ends of the spring are fixedly connected with the support plate and the bottom plate respectively, the vibrating motor and institute
It states support plate to be fixedly connected, the motor is connect with the shaft, and the top of the shaft is fixed on the bottom plate bottom center
Place.
Stem cell medium is placed in culture bottle, and culture bottle is placed in the placing groove on the board for putting, institute
Stating to place outside bottle can have the blocking mechanism to be stopped, and the vibrating motor vibration drives the support plate, and then drives institute
It states incubator to be vibrated, the culture bottle in the incubator is vibrated therewith, and the motor can drive the bottom
Plate is rotated, and the bottom plate carries out rotation and the incubator in the support plate is driven to be rotated, and culture bottle is turned
Dynamic, the present invention can make incubator while vibrate and rotate, and prevent culture solution from precipitating, and improve the uniformity of culture solution.
Preferably, the blocking mechanism includes fixed plate, baffle ring and connecting rod, and the fixed plate is fixed on the board for putting
Both ends, the placing groove top are equipped with the baffle ring corresponding with its position, and the adjacent baffle ring is fixed by the connecting rod
Connection, the fixed plate are fixedly connected with the baffle ring close to the fixed plate also by connecting rod.
Preferably, several link blocks being fixedly connected with the support plate, the incubator are additionally provided in the support plate
It is fixedly connected with the link block, the incubator is fixed in the support plate by the link block.
Preferably, door handle is further fixed on the dodge gate.
Preferably, several reinforcing rods are further fixed on the outer wall of the motor, the reinforcing rod is on the motor outer wall
It is uniformly distributed.
Preferably, the fixed link of several L-types is additionally provided on the outer wall of the shaft, one end of the fixed link is fixed on institute
It states on shaft outer wall, the other end of the fixed link is fixed on the bottom plate.
Preferably, knob is further fixed on the side wall of the board for putting, it is described to shake hands close to dodge gate.
The beneficial effects of the present invention are: stem cell medium is placed in culture bottle, culture bottle is placed on the board for putting
On the placing groove in, can there is the blocking mechanism to be stopped outside the placement bottle, the vibrating motor vibration drives institute
Support plate is stated, and then the incubator is driven to be vibrated, the culture bottle in the incubator is vibrated therewith, described
Motor can drive the bottom plate to be rotated, and the bottom plate carries out rotation and the incubator in the support plate is driven to carry out
Rotation, culture bottle are rotated, and the present invention can make incubator while vibrate and rotate, and prevent culture solution from precipitating, mention
The uniformity of high culture solution.
Detailed description of the invention
Attached drawing is used to provide further understanding of the present invention, and constitutes part of specification, with reality of the invention
It applies example to be used to explain the present invention together, not be construed as limiting the invention.In the accompanying drawings:
Fig. 1 is 1 structural schematic diagram of the embodiment of the present invention；
Fig. 2 is 1 schematic diagram of internal structure of the embodiment of the present invention；
Fig. 3 is 1 board for putting top view of the embodiment of the present invention；
Fig. 4 is 2 structural schematic diagram of the embodiment of the present invention；
Fig. 5 is 2 schematic diagram of internal structure of the embodiment of the present invention；
In the figure, it is marked as 1, incubator；11, dodge gate；111, door handle；2, sliding rail；3, board for putting；31, placing groove；
32, it shakes hands；4, blocking mechanism；41, fixed plate；42, baffle ring；43, connecting rod；5, support plate；51, link block；6, spring；7, bottom
Plate；8, vibrating motor；9, motor；91, reinforcing rod；10, shaft；101, fixed link；20, culture bottle.
Specific embodiment
A specific embodiment of the invention is described with reference to the accompanying drawing.
Embodiment 1
As shown in Figure 1-3, a kind of concussion frame of stem cell culture, including incubator 1, sliding rail 2, board for putting 3, blocking machine
Structure 4, support plate 5, spring 6, bottom plate 7, vibrating motor 8, motor 9 and shaft 10, the incubator 1 are equipped with dodge gate 11, institute
It states and is further fixed on door handle 111 on dodge gate 11, several spaced board for putting 3 up and down are equipped in the incubator 1, it is described
The sliding rail 2 on the inner wall corresponding with the incubator 1 is fixed on of the both ends of board for putting 3 is slidably connected, and the sliding rail 2 is cut
Face is concave shape, and the board for putting 3 is inserted into the groove of the concave shape of the sliding rail 2, and the blocking mechanism 4 is fixed on the pendulum
It puts on plate 3, the board for putting 3 is equipped with several equally distributed placing grooves 31, and the placing groove 31 can place culture bottle, described
Blocking mechanism 4 includes fixed plate 41, baffle ring 42 and connecting rod 43, and the fixed plate 41 is fixed on the both ends of the board for putting 3, institute
It states and is equipped with the baffle ring 42 corresponding with its position above placing groove 31, the baffle ring 42, which can cover, is being placed on the placing groove 31
Outside interior culture bottle 20, the adjacent baffle ring 42 is fixedly connected by the connecting rod 43, the fixed plate 4 and close to described solid
The baffle ring 42 of fixed board 4 is fixedly connected also by the connecting rod 43, the incubator 1, the support plate 5, several bullets
Spring 6 and the bottom plate 7 are fixedly connected sequentially from top to bottom, and the incubator 1 is fixed in the support plate 5, the spring 6
Both ends be fixedly connected respectively with the support plate 5 and the bottom plate 7, the spring 6 is in the support plate 5 and the bottom plate 7
Between be uniformly distributed, the vibrating motor 8 is fixedly connected with the support plate 5, and the motor 9 is connect with the shaft 10, institute
The top for stating shaft 10 is fixed at 7 bottom center of bottom plate, is further fixed on several reinforcing rods on the outer wall of the motor 9
91, the reinforcing rod 91 plays support fixed function to the motor 9, so that the motor 9 is stablized and supports the bottom plate 7, described
Reinforcing rod 91 is uniformly distributed on 9 outer wall of motor.
Several link blocks 51 being fixedly connected with the support plate 5, the link block 51 are additionally provided in the support plate 5
It is uniformly distributed in the support plate 5, the incubator 1 is fixedly connected with the link block 51, and the incubator 1 passes through institute
It states link block 51 to be fixed in the support plate 5, knob 32 is further fixed on the side wall of the board for putting 3, the knob 32 leans on
The nearly dodge gate 11.
Embodiment 2
As illustrated in figures 4-5, a kind of concussion frame of stem cell culture, on the basis of embodiment 1, the shaft 10
The fixed link 101 of several L-types is additionally provided on outer wall, one end of the fixed link 101 is fixed on 10 outer wall of shaft, described
The other end of fixed link 101 is fixed on the bottom plate 7, and the effect of the fixed link 101 is to play fixed support to the bottom plate 7
Effect can be such that the stabilization of bottom plate 7 is fixedly connected with 10 top of shaft, rotate the bottom plate 7 more stable.
Working method of the present invention: stem cell medium is placed in culture bottle 20, and culture bottle 20 is placed on the board for putting
In the placing groove 31 on 3, there can be the blocking mechanism 4 to be stopped outside the placement bottle 20, the baffle ring 42 just covers
Outside the culture bottle 20, prevent the culture bottle 20 from falling down when the incubator 1 vibrates and rotates, the vibrating motor 8
Vibration drives the support plate 5, and then the incubator 1 is driven to be vibrated, the culture bottle 20 in the incubator 1 with
Vibrated, the motor 9 can drive the bottom plate 7 to be rotated, and the bottom plate 7 carries out rotation and drives the support plate
The incubator 1 on 5 is rotated, and culture bottle 20 is rotated, and vibrating motor 8 and motor 9 can make incubator 1 while carry out
Vibration and rotation, prevent culture solution from precipitating, improve the uniformity of culture solution.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, although referring to aforementioned reality
Applying example, invention is explained in detail, for those skilled in the art, still can be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features.It is all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (7)
1. a kind of concussion frame of stem cell culture, which is characterized in that including incubator, sliding rail, board for putting, blocking mechanism, branch
Fagging, spring, bottom plate, vibrating motor, motor and shaft, the incubator are equipped with dodge gate, if being equipped in the incubator
Do spaced board for putting up and down, the cunning on the inner wall corresponding with the incubator is fixed on of the both ends of the board for putting
Rail is slidably connected, and the blocking mechanism is fixed on the board for putting, and the board for putting is equipped with several equally distributed placements
Slot, the incubator, the support plate, several springs and the bottom plate are fixedly connected sequentially from top to bottom, the culture
Case is fixed in the support plate, and the both ends of the spring are fixedly connected with the support plate and the bottom plate respectively, the vibration
Dynamic motor is fixedly connected with the support plate, and the motor is connect with the shaft, and the top of the shaft is fixed on the bottom
At plate bottom center.
2. a kind of concussion frame of stem cell culture according to claim 1, which is characterized in that the blocking mechanism includes
Fixed plate, baffle ring and connecting rod, the fixed plate are fixed on the both ends of the board for putting, are equipped with and its position above the placing groove
The corresponding baffle ring, the adjacent baffle ring are fixedly connected by the connecting rod, the fixed plate and the close fixed plate
The baffle ring be fixedly connected also by the connecting rod.
3. a kind of concussion frame of stem cell culture according to claim 1, which is characterized in that also set in the support plate
There are several link blocks being fixedly connected with the support plate, the incubator is fixedly connected with the link block, the incubator
It is fixed in the support plate by the link block.
4. a kind of concussion frame of stem cell culture according to claim 1, which is characterized in that also solid on the dodge gate
Surely there is door handle.
5. a kind of concussion frame of stem cell culture according to claim 1, which is characterized in that on the outer wall of the motor
Several reinforcing rods are further fixed on, the reinforcing rod is uniformly distributed on the motor outer wall.
6. a kind of concussion frame of stem cell culture according to claim 1, which is characterized in that on the outer wall of the shaft
It is additionally provided with the fixed link of several L-types, one end of the fixed link is fixed on the shaft outer wall, the other end of the fixed link
It is fixed on the bottom plate.
7. a kind of concussion frame of stem cell culture according to claim 1, which is characterized in that the side wall of the board for putting
On be further fixed on knob, it is described to shake hands close to dodge gate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811247531.5A CN109401963A (en) | 2018-10-25 | 2018-10-25 | A kind of concussion frame of stem cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811247531.5A CN109401963A (en) | 2018-10-25 | 2018-10-25 | A kind of concussion frame of stem cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109401963A true CN109401963A (en) | 2019-03-01 |
Family
ID=65469571
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811247531.5A Pending CN109401963A (en) | 2018-10-25 | 2018-10-25 | A kind of concussion frame of stem cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109401963A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112608846A (en) * | 2021-01-15 | 2021-04-06 | 吉林大学中日联谊医院 | Multifunctional cell culture device for improving premature ovarian failure stem cell exosomes |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101265466A (en) * | 2008-04-30 | 2008-09-17 | 天津理工大学 | Cultivating method used for coronary camber tissue under composite load and bioreactor thereof |
| WO2008133362A1 (en) * | 2007-04-28 | 2008-11-06 | Hyunjin Yang | Proliferation culture methods using micro-scaffolds for regulations of cell-to-cell signals |
| CN108048320A (en) * | 2017-12-23 | 2018-05-18 | 淮北智淮科技有限公司 | A kind of concussion frame of stem cell medium |
| CN108641898A (en) * | 2018-05-22 | 2018-10-12 | 苏州朝霞生物科技有限公司 | A kind of swinging shaking table for microculture |
-
2018
- 2018-10-25 CN CN201811247531.5A patent/CN109401963A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008133362A1 (en) * | 2007-04-28 | 2008-11-06 | Hyunjin Yang | Proliferation culture methods using micro-scaffolds for regulations of cell-to-cell signals |
| CN101265466A (en) * | 2008-04-30 | 2008-09-17 | 天津理工大学 | Cultivating method used for coronary camber tissue under composite load and bioreactor thereof |
| CN108048320A (en) * | 2017-12-23 | 2018-05-18 | 淮北智淮科技有限公司 | A kind of concussion frame of stem cell medium |
| CN108641898A (en) * | 2018-05-22 | 2018-10-12 | 苏州朝霞生物科技有限公司 | A kind of swinging shaking table for microculture |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112608846A (en) * | 2021-01-15 | 2021-04-06 | 吉林大学中日联谊医院 | Multifunctional cell culture device for improving premature ovarian failure stem cell exosomes |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN207109015U (en) | A kind of public health detection CO2gas incubator | |
| CN207703599U (en) | A kind of evenly mixing device for biological reagent | |
| CN106434288B (en) | Multiple field cyclotron oscillation device and its hanger | |
| CN108816734A (en) | A kind of tea screening device | |
| CN109401963A (en) | A kind of concussion frame of stem cell culture | |
| CN113602644B (en) | Blood transfusion branch of academic or vocational study is with platelet constant temperature oscillation case of preserving | |
| CN106669565A (en) | Anti-wall-adhering reaction kettle | |
| CN107258163A (en) | The accurate seeding apparatus of the special-shaped seed simple grain of mechanical revolving type | |
| CN111603990A (en) | Oscillation device for genetic engineering based on rotary centrifugal force | |
| JP2006320226A (en) | Cell culture apparatus | |
| CN113337381B (en) | Automatic grinding device and application thereof in obtaining single cells | |
| CN207123429U (en) | A kind of clinical laboratory's bacterial micro-organism sample prepares platform | |
| CN205774586U (en) | A kind of CO2 gas incubator | |
| CN214060537U (en) | Immune cell amplification culture instrument | |
| CN113862149B (en) | Shimmy integrated cell mechanics loading device | |
| KR20210007332A (en) | Medium culturing apparatus | |
| CN113559351B (en) | Liquid inlet and outlet balance weighing system capable of reducing swinging of infusion bag or urine bag | |
| CN209287704U (en) | A kind of full-automatic reciprocating sieve using physically screening | |
| CN207102679U (en) | A kind of student is with chemical experiment rack for test tube | |
| CN105195052A (en) | Shake-up device | |
| CN204205816U (en) | Eccentric block | |
| CN207918809U (en) | The culture dish of automatic subpackaging instrument shakes mechanism | |
| CN201746704U (en) | Drum washing machine shell | |
| CN205635568U (en) | Automatic cultivation oscillator of unpacking door | |
| CN208356982U (en) | Carboxymethyl guar rubber powder production screening ground and mixed device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190301 |
|
| RJ01 | Rejection of invention patent application after publication |