CN219526661U

CN219526661U - Cell culture device

Info

Publication number: CN219526661U
Application number: CN202320583515.3U
Authority: CN
Inventors: 吴亚剑
Original assignee: Western Medical Science And Technology Group Co ltd
Current assignee: Western Medical Science And Technology Group Co ltd
Priority date: 2023-03-23
Filing date: 2023-03-23
Publication date: 2023-08-15
Anticipated expiration: 2033-03-23

Abstract

The utility model discloses a cell culture device which comprises a box body, wherein the upper end of the box body is provided with a box cover, the upper end face of the box cover is symmetrically provided with two liquid inlet cylinders, the two liquid inlet cylinders are respectively provided with a rotary cover, the front surface of the box cover is provided with a mounting insert block, the front surface of the box body is provided with a mounting seat, a culture plate is arranged in the box body, the lower end of the culture plate is provided with a rotary assembly, a plurality of placing grooves are formed in the culture plate, clamping assemblies are arranged in the placing grooves, a culture dish is clamped by the clamping assemblies, and the mounting seat is provided with a mounting assembly. According to the utility model, through the matched use of the motor, the rotating shaft, the driving gear, the driven gear, the rotating shaft, the bearing, the culture plate, the placing groove and the culture dish, the operation is simple and convenient, bacteria in air are prevented from entering the culture dish, so that the cells are prevented from being polluted, and through the matched use of the first cavity, the limiting rod, the first spring, the outer clamping plate, the inner clamping plate and the connecting rod, the culture dishes with different sizes can be compacted and fixed, and the damage caused by the movement of the culture dishes in the use process is avoided.

Description

Cell culture device

Technical Field

The utility model relates to the technical field of cell culture, in particular to a cell culture device.

Background

Cell culture refers to a method for simulating in vitro in vivo environment (asepsis, proper temperature, pH value, a certain nutrition condition and the like) to enable the cells to survive, grow, reproduce and maintain main structures and functions, and is also called a cell cloning technology, a normal term in biology is a cell culture technology, the cell culture is an essential process for the whole bioengineering technology or one of the bioengineering technologies, the cell culture is a large-scale cloning of cells, the cell culture technology can be a simple single cell or a few differentiated multiple cells from one cell through a large-scale culture, the cloning technology is an essential link, and the cell culture is a cloning of cells. Cell culture techniques are important and commonly used techniques in cell biology research methods, by which a large number of cells can be obtained, and by which signal transduction of cells, anabolism of cells, growth and proliferation of cells, etc. can be studied.

When the existing cell culture device is used, cells are usually placed in a culture dish, when nutrient solution needs to be added, a sealed container is required to be opened or the culture dish is required to be taken out, the nutrient solution is manually added to the culture dish one by one, the operation is inconvenient, impurities in the air can fall into the culture dish to cause pollution, meanwhile, the existing cell culture device cannot fix the culture dish with different sizes, and then the culture dish is easy to move, so that the culture dish collides, and the requirements of a user cannot be met. For this purpose, a corresponding technical solution needs to be designed to solve.

Disclosure of Invention

(one) solving the technical problems

In order to overcome the defects in the prior art, the utility model provides a cell culture device which solves the problems in the prior art.

(II) technical scheme

In order to achieve the above purpose, the utility model is realized by the following technical scheme:

the cell culture device comprises a box body, wherein the upper end of the box body is provided with a box cover, the upper end face of the box cover is symmetrically provided with two liquid inlet cylinders, the two liquid inlet cylinders are respectively provided with a rotary cover, the front surface of the box cover is provided with a mounting insert block, the front surface of the box body is provided with a mounting seat, a culture plate is arranged in the box body, the lower end of the culture plate is provided with a rotary assembly, a plurality of placing grooves are formed in the culture plate, clamping assemblies are arranged in the placing grooves, culture dishes are clamped by the clamping assemblies, and the mounting seat is provided with a mounting assembly;

the clamping assembly comprises a first cavity arranged inside the culture plate, a limiting rod is arranged in the first cavity, a first spring is sleeved on the limiting rod, an outer clamping plate is fixed at one end, extending out of the first cavity, of the limiting rod, the outer clamping plate is positioned in the placing groove, connecting rods are symmetrically arranged on the outer clamping plate, one end of each connecting rod penetrates through the outer clamping plate and is fixedly connected with an inner clamping plate, and a damping spring is sleeved on each connecting rod;

the rotary assembly comprises a rotary motor arranged at the bottom of the box body, the output end of the rotary motor is fixedly connected with a rotary shaft, a driving gear is fixedly connected to the rotary shaft, driven gears are arranged at the left end and the right end of the driving gear, a rotary shaft is fixedly inserted on the driven gears, the upper end of the rotary shaft is fixedly connected with the culture plate, and a bearing is arranged at the lower end of the rotary shaft;

the installation component is including seting up the second cavity in the mount pad, be equipped with the inserted bar in the second cavity, the one end fixedly connected with pull ring that the inserted bar extended the second cavity, cup jointed the second spring on the inserted bar.

Preferably, the upper end of the liquid inlet cylinder is provided with a first liquid inlet, the upper end of the rotary cover is provided with a second liquid inlet, and the calibers of the first liquid inlet and the second liquid inlet are consistent.

Preferably, a slot matched with the inserted link is arranged on the outer side wall of the installation inserted block.

Preferably, a rubber pad is paved on the inner side wall of the inner clamping plate.

Preferably, the hinge seat is fixedly connected with the joint of the back surfaces of the box body and the box cover.

(III) beneficial effects

The utility model discloses cell culture device uses through the cooperation of motor, the pivot, the driving gear, driven gear, the rotation axis, a bearing, the culture plate, standing groove and culture dish, it rotates to drive the culture plate through the motor intermittent type, and add the nutrient solution to the culture dish through feed liquor section of thick bamboo one by one, easy operation is convenient, avoid bacterium in the air to get into the inside of culture dish, thereby avoid the cell to receive the pollution, guarantee the culture of cell, through setting up first cavity, the gag lever post, first spring, the cooperation of outer splint, interior splint and connecting rod, can realize compressing tightly fixedly the culture dish of equidimension not, avoid the culture dish drunkenness and damage in the use.

Drawings

The accompanying drawings are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate the utility model and together with the embodiments of the utility model, serve to explain the utility model.

In the drawings:

FIG. 1 is a schematic diagram of the overall structure of a cell culture apparatus according to the utility model;

fig. 2 is a schematic view of the structure of the back of the case according to the present utility model.

Fig. 3 is a schematic view of the structure of the inside of the case according to the present utility model.

Fig. 4 is a schematic view of the structure of the rotary assembly according to the present utility model.

Fig. 5 is a schematic structural view of the fixing assembly according to the present utility model.

Fig. 6 is a schematic view of the structure of the inside of the mounting base according to the present utility model.

In the figure: 1-box, 2-box cover, 3-mount pad, 4-mount insert, 5-feed cylinder, 6-first feed inlet, 7-rotary cover, 8-second feed inlet, 9-articulated seat, 10-culture plate, 11-standing groove, 12-culture dish, 13-rotating electrical machine, 14-pivot, 15-driving gear, 16-driven gear, 17-pivot, 18-bearing, 19-outer splint, 20-inner splint, 21-connecting rod, 22-damping spring, 23-first cavity, 24-gag lever post, 25-first spring, 26-rubber pad, 27-insert rod, 28-slot, 29-second cavity, 30-second spring, 31-pull ring.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1-6, the present utility model provides a technical solution: a cell culture apparatus comprising: the culture box comprises a box body 1, wherein a box cover 2 is arranged at the upper end of the box body 1, two liquid inlet cylinders 5 are symmetrically arranged on the upper end face of the box cover 2, rotary covers 7 are arranged on the two liquid inlet cylinders 5, an installation insert block 4 is arranged on the front face of the box cover 2, an installation seat 3 is arranged on the front face of the box body 1, a culture plate 10 is arranged in the box body 1, a rotary assembly is arranged at the lower end of the culture plate 10, a plurality of placing grooves 11 are formed in the culture plate 10, clamping assemblies are arranged in the placing grooves 11, culture dishes 12 are clamped by the clamping assemblies, and an installation assembly is arranged on the installation seat 3;

the clamping assembly comprises a first cavity 23 arranged inside the culture plate 10, a limiting rod 24 is arranged in the first cavity 23, a first spring 25 is sleeved on the limiting rod 24, an outer clamping plate 19 is fixed at one end, extending out of the first cavity 23, of the limiting rod 24, the outer clamping plate 19 is positioned in the placing groove 11, connecting rods 21 are symmetrically arranged on the outer clamping plate 19, one end of each connecting rod 21 penetrates through the outer clamping plate 19 and is fixedly connected with an inner clamping plate 20, and a damping spring 22 is sleeved on each connecting rod 21;

the rotating assembly comprises a rotating motor 13 arranged at the bottom of the box body 1, the output end of the rotating motor 13 is fixedly connected with a rotating shaft 14, a driving gear 15 is fixedly connected to the rotating shaft 14, driven gears 16 are arranged at the left end and the right end of the driving gear 15, a rotating shaft 12 is fixedly inserted on the driven gears 16, the upper end of the rotating shaft 17 is fixedly connected with the culture plate 10, and a bearing 18 is arranged at the lower end of the rotating shaft 17;

the installation component is including seting up the second cavity 29 in mount pad 3, is equipped with inserted bar 27 in the second cavity 29, and the one end fixedly connected with pull ring 31 of inserted bar 27 extension second cavity 29 has cup jointed second spring 30 on the 27 inserted bar.

Further improved, as shown in fig. 1: the first inlet 6 has been seted up to feed liquor section of thick bamboo 5 upper end, and second inlet 8 has been seted up to rotatory lid 7 upper end, and the bore of first inlet 6 is unanimous with second inlet 8, when rotatory first inlet 6 of rotatory lid 7 and second inlet 8 coincide, can also add the culture dish 12 through feed liquor section of thick bamboo 5 with cultivateing through the inlet in.

Further improved, as shown in fig. 6: the slot 28 matched with the inserted link 27 is formed in the outer side wall of the mounting inserted block 4, and the box cover 2 is conveniently fixed on the box body 1 by arranging the inserted link 27 and the slot 28, so that the box cover 2 can be conveniently opened and closed.

Further improved, as shown in fig. 5: the rubber pad 26 has been laid on the inside wall of interior splint 20, through setting up rubber pad 26, can effectually protect culture dish 12, avoids culture dish 12 to be pressed from both sides bad by clamping assembly.

Further improved, as shown in fig. 2: the back of the junction of the box body 1 and the box cover 2 is fixedly connected with a hinge seat 9, and the back of the junction of the box body 1 and the box cover 2 is connected through the hinge seat 9, so that the box body 1 and the box cover 2 are connected together.

Working principle: when the culture dish 12 is used, nutrient solution is added into the culture dish 12 through the liquid inlet cylinder 5, then the rotating motor 13 is started, the rotating motor 13 drives the rotating shaft 14 to rotate, the rotating shaft 14 drives the driving gear 15 to rotate, the driving gear 15 drives the driven gear 16 to rotate, thereby driving the rotating shaft 17 to rotate, further driving the culture dish 12 on the culture plate 10 to rotate, the nutrient solution can be added into the culture dish 12 one by one through intermittent driving of the rotating motor 13, manual nutrient solution addition into the culture dish 12 one by one is avoided, the operation is simple and convenient, bacteria in the air are prevented from entering the culture dish 12, so that cells are prevented from being polluted, the culture of the cells is ensured, the limit rod 24 is driven to move in the first cavity 23 by pulling the outer clamp plate 19 outwards, the limit rod 25 is extruded to deform, then the culture dish 12 is placed into the placing groove 11, the outer clamp plate 19 is loosened, the first spring 25 is restored to deform, the outer clamp plate 19 is pushed to clamp the culture dish 12, the culture dish 12 is clamped one by one, the culture dish 12 with different sizes is prevented from being damaged, and the culture dish 12 is prevented from being damaged by the clamp assembly.

And (3) notes: the motor model related by the utility model is 5IK90 GN-YF.

While the fundamental and principal features of the utility model and advantages of the utility model have been shown and described, it will be apparent to those skilled in the art that the utility model is not limited to the details of the foregoing exemplary embodiments, but may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.

Claims

1. A cell culture apparatus, characterized in that: the novel culture dish box comprises a box body (1), wherein a box cover (2) is arranged at the upper end of the box body (1), two liquid inlet barrels (5) are symmetrically arranged on the upper end face of the box cover (2), rotary covers (7) are arranged on the two liquid inlet barrels (5), mounting inserting blocks (4) are arranged on the front face of the box cover (2), mounting seats (3) are arranged on the front face of the box body (1), a culture plate (10) is arranged in the box body (1), rotary components are arranged at the lower end of the culture plate (10), a plurality of placing grooves (11) are formed in the culture plate (10), clamping components are arranged in the placing grooves (11), culture dishes (12) are clamped by the clamping components, and mounting components are arranged on the mounting seats (3);

the clamping assembly comprises a first cavity (23) arranged inside the culture plate (10), a limiting rod (24) is arranged in the first cavity (23), a first spring (25) is sleeved on the limiting rod (24), an outer clamping plate (19) is fixed at one end, extending out of the first cavity (23), of the limiting rod (24), the outer clamping plate (19) is positioned in the placing groove (11), connecting rods (21) are symmetrically arranged on the outer clamping plate (19), one end of each connecting rod (21) penetrates through the outer clamping plate (19) and is fixedly connected with an inner clamping plate (20), and damping springs (22) are sleeved on the connecting rods (21);

the rotary assembly comprises a rotary motor (13) arranged at the bottom of the box body (1), the output end of the rotary motor (13) is fixedly connected with a rotary shaft (14), a driving gear (15) is fixedly connected to the rotary shaft (14), driven gears (16) are respectively arranged at the left end and the right end of the driving gear (15), a rotary shaft (17) is fixedly inserted on the driven gears (16), the upper end of the rotary shaft (17) is fixedly connected with the culture plate (10), and a bearing (18) is arranged at the lower end of the rotary shaft (17);

the mounting assembly comprises a second cavity (29) formed in the mounting seat (3), an inserting rod (27) is arranged in the second cavity (29), one end, extending out of the second cavity (29), of the inserting rod (27) is fixedly connected with a pull ring (31), and a second spring (30) is sleeved on the inserting rod (27).

2. A cell culture apparatus according to claim 1, wherein: the upper end of the liquid inlet barrel (5) is provided with a first liquid inlet (6), the upper end of the rotary cover (7) is provided with a second liquid inlet (8), and the calibers of the first liquid inlet (6) and the second liquid inlet (8) are consistent.

3. A cell culture apparatus according to claim 1, wherein: the outer side wall of the mounting plug block (4) is provided with a slot (28) matched with the plug rod (27).

4. A cell culture apparatus according to claim 1, wherein: a rubber pad (26) is paved on the inner side wall of the inner clamping plate (20).

5. A cell culture apparatus according to claim 1, wherein: the hinge seat (9) is fixedly connected at the back connection part of the box body (1) and the box cover (2).