CN218026116U

CN218026116U - Pressurizing device for in vitro cell culture

Info

Publication number: CN218026116U
Application number: CN202221667470.XU
Authority: CN
Inventors: 陈飞; 李涛; 孙铭通; 彭福帅
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-06-29
Filing date: 2022-06-29
Publication date: 2022-12-13
Anticipated expiration: 2032-06-29

Abstract

The utility model relates to the technical field of cell culture, in particular to a pressurizing device for in vitro cell culture. The device comprises a bottom plate, wherein a fixed box, a piston box and an incubator are arranged on the bottom plate, a supporting plate is arranged on one side of the fixed box, a driving motor is arranged on the supporting plate, a threaded rod is arranged at the output end of the driving motor, a threaded sleeve is sleeved on the threaded rod, a guide plate is arranged at the bottom of the threaded sleeve, a guide rod is arranged on one side of the guide plate, a piston is arranged at one end of the guide rod, and the piston box is communicated with the incubator through a sealing pipe. The utility model provides a cell pressure device with simple operation, accurate control and stable performance, which can pressurize the cultured cells in vitro and control the pressurizing size, time and the like.

Description

Pressurizing device for in vitro cell culture

Technical Field

The utility model relates to the technical field of cell culture, in particular to a pressurizing device for in vitro cell culture.

Background

Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained by cell culture, and signal transduction of cells, anabolism of cells, growth and proliferation of cells and the like can be researched by the cell culture techniques.

When in vitro cell culture, cells need to be stimulated by changing pressure to form a pressure surrounding environment, and the reaction of the cells in a pressure state is researched. However, in the conventional air pressure pressurizing incubator, high-pressure gas needs to be continuously filled to maintain the pressure in the incubator, so that the gas in the incubator is always in a flowing state, which is not beneficial to continuously and stably pressurizing cells, and the continuous flowing of the gas can cause the temperature in the incubator to be uneven, the change is large, and the constant temperature is poor.

SUMMERY OF THE UTILITY MODEL

The utility model provides a cell pressure device with simple operation, accurate control and stable performance, which can pressurize the cultured cells in vitro and control the pressurizing size, time and the like.

The utility model provides a technical scheme does: a pressurizing device for in vitro cell culture, which is characterized in that: the bottom plate, be equipped with fixed case, piston box, incubator on the bottom plate, fixed case one side is equipped with rather than fixed connection's backup pad, be equipped with driving motor in the backup pad, driving motor's output is equipped with the threaded rod that runs through fixed case lateral wall, the cover is equipped with the thread bush on the threaded rod, the thread bush bottom is equipped with the deflector, deflector one side is equipped with the guide bar that runs through fixed case lateral wall and piston box lateral wall, guide bar one end is equipped with the piston, be linked together through the sealed tube between piston box and the incubator.

The top of the fixed box is provided with a control box, the control box is provided with a control button, a controller is arranged in the control box, and the controller, the control button and the driving motor are electrically connected.

And one end of the threaded rod is provided with a bearing.

The side wall of one side of the fixed box is provided with a through hole A for the threaded rod to pass through, and the side wall of the other side of the fixed box is provided with a mounting groove for the bearing to be mounted and a through hole B for the guide rod to pass through.

The surface of the threaded rod is provided with external threads, and the inner wall of the threaded sleeve is provided with internal threads meshed with the external threads.

The bottom of the guide plate is provided with a sliding block, and the bottom wall of the fixed box is provided with a sliding rail for the sliding block to slide.

The piston is attached to the inner wall of the piston box.

Transparent glass is embedded on the front wall of the incubator.

Compared with the prior art, the utility model the advantage lie in: the utility model discloses threaded rod and thread bush intermeshing, through driving motor's drive, realize the motion of piston to make the pressure change in the incubator, make the cell in the incubator form corresponding pressure. The utility model has the advantages of being simple in structure and easy operation, control is accurate, the stable performance, has very big using value and spreading value.

Drawings

FIG. 1 is a front view of the pressurizing device for in vitro cell culture according to the present invention;

FIG. 2 is a schematic view of a partial structure of a pressurizing device for in vitro cell culture according to the present invention.

As shown in the figure: 1. a base plate; 2. a fixed box; 3. a piston case; 4. an incubator; 5. a support plate; 6. a drive motor; 7. a threaded rod; 8. a threaded sleeve; 9. a guide plate; 10. a guide bar; 11. a piston; 12. a sealing tube; 13. a control box; 14. a control button; 15. a bearing; 16. a slider; 17. a slide rail; 18. and (4) transparent glass.

Detailed Description

The following will explain the pressurizing device for in vitro cell culture according to the present invention in detail with reference to the attached drawings.

The utility model provides a pressurizing device for in vitro cell culture, which is shown in figure 1 and figure 2 and is characterized in that: including bottom plate 1, be equipped with fixed case 2, piston box 3, incubator 4 on bottom plate 1, 2 one side of fixed case are equipped with backup pad 5 rather than fixed connection, be equipped with driving motor 6 on backup pad 5, driving motor 6's output is equipped with the threaded rod 7 that runs through 2 lateral walls of fixed case, the cover is equipped with thread bush 8 on the threaded

rod

7, 8 bottoms of thread bush are equipped with deflector 9, deflector 9 one side is equipped with the guide bar 10 that runs through 2 lateral walls of fixed case and 3 lateral walls of piston box, guide bar 10 one end is equipped with piston 11, be linked together through sealed tube 12 between piston box 3 and the incubator 4.

Fixed 2 tops of case are equipped with control box 13, be equipped with control button 14 on the control box 13, be equipped with the controller in the control box 13, controller, control button 14, driving motor 6 electric connection.

One end of the threaded rod 7 is provided with a bearing 15.

The side wall of one side of the fixed box 2 is provided with a through hole A for the threaded rod 7 to pass through, and the side wall of the other side of the fixed box 2 is provided with a mounting groove for the bearing 15 to be mounted and a through hole B for the guide rod 10 to pass through.

The surface of the threaded rod 7 is provided with an external thread, and the inner wall of the threaded sleeve 8 is provided with an internal thread meshed with the external thread.

The bottom of the guide plate 9 is provided with a slide block 16, and the bottom wall of the fixed box 2 is provided with a slide rail 17 for the slide block 16 to slide.

The piston 11 is attached to the inner wall of the piston box 3.

Transparent glass 18 is embedded on the front wall of the incubator 4.

The utility model discloses when concrete implementation, will cultivate in vitro, the cell that grows well is put into through high temperature high pressure disinfection's incubator 4 together with the culture that maintains its external living environment, through sealed tube 12 with piston box 3 and 4 sealing connection of incubator, simultaneously

piston

11 and 3 inner walls of piston box laminate mutually, thereby form stable airtight environment, then start through controller control driving motor 6, driving motor 6 drives threaded rod 7 and rotates, threaded rod 7 rotates and drives 8 slides of thread bush, 8 slides and drive deflector 9 slides of thread bush, deflector 9 slides and drives piston 11 and slide in piston box 3 through guide bar 10, thereby make the atmospheric pressure change in the incubator 4, make the cell in the incubator 4 receive corresponding pressure effect.

The present invention and the embodiments thereof have been described above, but the description is not limited thereto, and the embodiment shown in the drawings is only one of the embodiments of the present invention, and the actual structure is not limited thereto. In summary, it should be understood that those skilled in the art should also understand the scope of the present invention without inventively designing the similar structure and embodiments of the present invention without departing from the spirit of the present invention.

Claims

1. A pressurizing device for in vitro cell culture, which is characterized in that: including bottom plate (1), be equipped with fixed case (2), piston case (3), incubator (4) on bottom plate (1), fixed case (2) one side is equipped with backup pad (5) rather than fixed connection, be equipped with driving motor (6) on backup pad (5), the output of driving motor (6) is equipped with threaded rod (7) that runs through fixed case (2) lateral wall, the cover is equipped with thread bush (8) on threaded rod (7), thread bush (8) bottom is equipped with deflector (9), deflector (9) one side is equipped with guide bar (10) that run through fixed case (2) lateral wall and piston case (3) lateral wall, guide bar (10) one end is equipped with piston (11), be linked together through sealed tube (12) between piston case (3) and incubator (4).

2. The pressure device for in vitro cell culture according to claim 1, wherein: fixed case (2) top is equipped with control box (13), be equipped with control button (14) on control box (13), be equipped with the controller in control box (13), controller, control button (14), driving motor (6) electric connection.

3. The pressurizing device for in vitro cell culture according to claim 1, wherein: and one end of the threaded rod (7) is provided with a bearing (15).

4. The pressure device for in vitro cell culture according to claim 1, wherein: the screw rod fixing device is characterized in that a through hole A for a threaded rod (7) to pass through is formed in the side wall of one side of the fixing box (2), and a mounting groove for a bearing (15) to be mounted and a through hole B for a guide rod (10) to pass through are formed in the side wall of the other side of the fixing box (2).

5. The pressurizing device for in vitro cell culture according to claim 1, wherein: the surface of the threaded rod (7) is provided with an external thread, and the inner wall of the thread sleeve (8) is provided with an internal thread meshed with the external thread.

6. The pressurizing device for in vitro cell culture according to claim 1, wherein: the bottom of the guide plate (9) is provided with a sliding block (16), and the bottom wall of the fixed box (2) is provided with a sliding rail (17) for the sliding block (16) to slide.

7. The pressurizing device for in vitro cell culture according to claim 1, wherein: and the piston (11) is attached to the inner wall of the piston box (3).

8. The pressure device for in vitro cell culture according to claim 1, wherein: transparent glass (18) is embedded on the front wall of the incubator (4).