CN210796493U

CN210796493U - Suspension cell culture bottle

Info

Publication number: CN210796493U
Application number: CN201921341955.8U
Authority: CN
Inventors: 崔姣艳; 张晓龙; 王新荣
Original assignee: Xi'an New Chang'an Regenerative Medicine Technology Research And Development Co ltd
Current assignee: Xi'an New Chang'an Regenerative Medicine Technology Research And Development Co ltd
Priority date: 2019-08-16
Filing date: 2019-08-16
Publication date: 2020-06-19
Anticipated expiration: 2029-08-16

Abstract

The utility model discloses a suspension cell culture bottle, which belongs to the technical field of suspension cell culture, and particularly relates to a suspension cell culture bottle, which comprises a base, a bottle body, a motor, a temperature sensor and an air pump, wherein the left side of the top of the base is fixedly provided with a left bottle body and a right bottle body, the middle of the top of the bottle body is provided with a top cover, the left side and the right side of the top of the bottle body are fixedly provided with a port pipe, the top of the top cover is fixedly provided with the motor, the output end of the bottom of the motor penetrates through the top cover to be provided with a rotating shaft, the surface of the rotating shaft is fixedly provided with a stirring rod, the left side and the right side of the stirring rod are fixedly provided with stirring blades, the structure is reasonable, the suspension cells are cultured by matching the bottle body, the top cover, through the cooperation of temperature sensor and display screen, realized real-time temperature monitoring, improved and cultivateed efficiency.

Description

Suspension cell culture bottle

Technical Field

The utility model relates to a suspension cell culture technical field specifically is a suspension cell culture bottle.

Background

Suspension culture refers to a tissue culture system for culturing single cells and small cell clusters in a liquid culture medium which is constantly stirred or shaken, and is a culture mode of anchorage-independent cells. Certain anchorage-dependent cells can also be cultured using this method, after adaptation and selection. It is relatively simple to increase the scale of suspension culture, as long as the volume is increased. The depth is more than 5mm, the culture medium is required to be stirred and more than 10cm, and carbon dioxide and air are required to be deeply introduced to ensure sufficient gas exchange. The cells are always in a dispersed suspension in the culture solution by shaking or rotating the device.

The existing suspension cell culture bottle has some defects in the using process, for example, gas is inconvenient to be introduced into the bottom of the bottle, and the temperature monitoring function is not available, so that a novel suspension cell culture bottle needs to be developed to solve the problems.

SUMMERY OF THE UTILITY MODEL

This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and in the abstract of the specification and the title of the application to avoid obscuring the purpose of this section, the abstract of the specification and the title of the application, and such simplifications or omissions are not intended to limit the scope of the invention.

The utility model discloses in view of the problem that exists among the current suspension cell culture bottle, provided.

Therefore, the utility model aims at providing a suspension cell culture bottle can realize at the in-process that uses, conveniently carries the gas of different grade type to the bottle, and has real-time temperature monitoring function, guarantees to cultivate efficiency.

For solving the technical problem, according to the utility model discloses an aspect, the utility model provides a following technical scheme:

the utility model provides a suspension cell culture bottle, its includes base, bottle, motor, temperature sensor and air pump, base top left side fixed mounting has the bottle, be provided with the top cap in the middle of the bottle top, bottle top left side and right side fixed mounting have the mouth of branch pipe, top cap top fixed mounting has the motor, motor bottom output runs through the top cap and is provided with the pivot, pivot fixed surface installs the puddler, puddler left and right sides fixed mounting has stirring vane.

As the utility model discloses a preferred scheme of suspension cell culture bottle, wherein: the temperature sensor is fixedly mounted at the bottom of the left side wall of the inner cavity of the bottle body, the display screen is fixedly mounted at the bottom of the left side wall of the bottle body, and the display screen is electrically connected with the temperature sensor.

As the utility model discloses a preferred scheme of suspension cell culture bottle, wherein: the base top right side fixed mounting has the air pump, air pump top left side fixed mounting has the air pipe connector, air pump top right side fixed mounting has the carbon dioxide pipe connector, just the air pump passes through the connecting pipe and is connected with the bottle.

As the utility model discloses a preferred scheme of suspension cell culture bottle, wherein: the middle of the bottom of the bottle body penetrates through the base and is provided with a discharge port, the opening pipe and the discharge port are fixedly provided with a sealing cover, and the opening pipe and the inner cavity of the top cover are fixedly provided with a hydrophobic breathable film.

As the utility model discloses a preferred scheme of suspension cell culture bottle, wherein: and supporting foot frames are fixedly arranged at four corners of the bottom of the base, and suckers are fixedly arranged at the bottoms of the supporting foot frames.

Compared with the prior art, the beneficial effects of the utility model are that: through the setting of this kind of suspension cell culture bottle, structural design is reasonable, has realized the cultivation to suspension cell through the cooperation of bottle, top cap, motor, pivot, puddler, stirring vane, through the cooperation of air pump, air pipe connection and carbon dioxide coupling, has made things convenient for and has carried different gas to the bottle end, guarantees sufficient gas exchange, through the cooperation of temperature sensor and display screen, has realized real-time temperature monitoring, improves and cultivates efficiency.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the present invention will be described in detail with reference to the accompanying drawings and detailed embodiments, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive labor. Wherein:

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic top view of the present invention;

fig. 3 is a schematic view of the top cover of the present invention.

In the figure; 100 bases, 110 supporting legs, 120 suckers, 200 bottles, 210 top covers, 220 split pipes, 230 discharge ports, 240 hydrophobic and breathable films, 300 motors, 310 rotating shafts, 320 stirring rods, 330 stirring blades, 400 temperature sensors, 410 display screens, 500 air pumps, 510 air pipe joints and 520 carbon dioxide pipe joints.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described herein, and it will be apparent to those skilled in the art that the present invention may be practiced without departing from the spirit and scope of the invention.

Next, the present invention will be described in detail with reference to the schematic drawings, and in the detailed description of the embodiments of the present invention, for convenience of explanation, the sectional view showing the device structure will not be enlarged partially according to the general scale, and the schematic drawings are only examples, and should not limit the scope of the present invention. In addition, the three-dimensional dimensions of length, width and depth should be included in the actual fabrication.

In order to make the objects, technical solutions and advantages of the present invention clearer, embodiments of the present invention will be described in further detail below with reference to the accompanying drawings.

The utility model provides a following technical scheme: a suspension cell culture bottle is convenient to convey different types of gas to a bottle body in the using process, has a real-time temperature monitoring function, and ensures the cultivation efficiency, please refer to FIG. 1, which comprises a base 100, a bottle body 200, a motor 300, a temperature sensor 400 and an air pump 500;

referring to fig. 1 again, the four corners of the bottom of the base 100 are fixedly provided with the supporting legs 110, the bottom of the supporting legs 110 is fixedly provided with the suckers 120, specifically, the supporting legs 110 are welded at the four corners of the bottom of the base 100, the suckers 120 are sleeved at the bottom of the supporting legs 110, the base 100 is used for bearing the bottle 200 and the air pump 500, the supporting legs 110 are used for supporting the base 100, and the suckers 120 are used for increasing the stability;

referring to fig. 1 again, a bottle body 200 is fixedly mounted on the left side of the top of the base 100, a top cover 210 is arranged in the middle of the top of the bottle body 200, a tapping pipe 220 is fixedly mounted on the left side and the right side of the top of the bottle body 200, a hydrophobic breathable film 240 is fixedly mounted in the inner cavities of the tapping pipe 220 and the top cover 210, specifically, the bottle body 200 is adhered to the left side of the top of the base 100, the top cover 210 is adhered to the middle of the top of the bottle body 200, the tapping pipe 220 is inserted in the left side and the right side of the top of the bottle body 200, the hydrophobic breathable film 240 is clamped in the inner cavities of the tapping pipe 220 and the top cover 210, the bottle body 200 is used for bearing a culture medium and;

referring to fig. 1 again, a motor 300 is fixedly installed at the top of the top cover 210, a rotating shaft 310 is arranged at the output end of the bottom of the motor 300 and penetrates through the top cover 210, a stirring rod 320 is fixedly installed on the surface of the rotating shaft 310, stirring blades 330 are fixedly installed on the left side and the right side of the stirring rod 320, specifically, the motor 300 is screwed in the middle of the top cover 210, the output end of the bottom of the motor 300 and penetrates through the top cover 210 and is screwed with the rotating shaft 310, the stirring rod 320 is welded on the surface of the rotating shaft 310, the stirring blades 330 are adhered to the two sides of the stirring rod 320, the motor 300 is a low-rotation-speed motor 300 and is used for providing power, the rotating shaft 310 is used for bearing;

referring to fig. 1 again, the temperature sensor 400 is bonded to the bottom of the left side wall of the inner cavity of the bottle body 200, the display screen 410 is bonded to the bottom of the left side wall of the bottle body 200, and the display screen 410 is electrically connected to the temperature sensor 400;

referring to fig. 1 again, an air pump 500 is fixedly installed on the right side of the top of the base 100, an air pipe joint 510 is fixedly installed on the left side of the top of the air pump 500, a carbon dioxide pipe joint 520 is fixedly installed on the right side of the top of the air pump 500, and the air pump 500 is connected with the bottle body 200 through a connecting pipe.

The working principle is as follows: in the process of using the suspension cell culture bottle, firstly, the culture medium and the cells are injected through the tapping pipe 220, the culture of the suspension cells is realized through the matching of the bottle body 200, the top cover 210, the motor 300, the rotating shaft 310, the stirring rod 320 and the stirring blades 330, different gases are conveniently conveyed to the bottom of the bottle through the matching of the air pump 500, the air pipe joint 510 and the carbon dioxide pipe joint 520, sufficient gas exchange is ensured, through the matching of the temperature sensor 400 and the display screen 410, the real-time temperature monitoring is realized, and the culture efficiency is improved.

While the invention has been described above with reference to an embodiment, various modifications may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention. In particular, as long as there is no structural conflict, the various features of the disclosed embodiments of the present invention can be used in any combination with each other, and the non-exhaustive description of these combinations in this specification is merely for the sake of brevity and resource conservation. Therefore, it is intended that the invention not be limited to the particular embodiments disclosed, but that the invention will include all embodiments falling within the scope of the appended claims.

Claims

1. A suspension cell culture bottle, which is characterized in that: including base (100), bottle (200), motor (300), temperature sensor (400) and air pump (500), base (100) top left side fixed mounting has bottle (200), be provided with in the middle of bottle (200) top cap (210), bottle (200) top left side and right side fixed mounting have branch mouth pipe (220), top cap (210) top fixed mounting has motor (300), motor (300) bottom output runs through top cap (210) and is provided with pivot (310), pivot (310) fixed surface installs puddler (320), puddler (320) left and right sides fixed mounting has stirring vane (330).

2. A suspension cell culture flask according to claim 1, wherein: bottle (200) inner chamber left side wall bottom fixed mounting has temperature sensor (400), bottle (200) left side wall bottom fixed mounting has display screen (410), just display screen (410) and temperature sensor (400) electric connection.

3. A suspension cell culture flask according to claim 1, wherein: base (100) top right side fixed mounting has air pump (500), air pump (500) top left side fixed mounting has air pipe connector (510), air pump (500) top right side fixed mounting has carbon dioxide pipe connector (520), just air pump (500) are connected with bottle (200) through the connecting pipe.

4. A suspension cell culture flask according to claim 1, wherein: the middle of the bottom of the bottle body (200) penetrates through the base (100) to be provided with a discharge port (230), a sealing cover is fixedly mounted on the opening pipe (220) and the discharge port (230), and a hydrophobic breathable film (240) is fixedly mounted in an inner cavity of the opening pipe (220) and the top cover (210).

5. A suspension cell culture flask according to claim 1, wherein: four corners of the bottom of the base (100) are fixedly provided with supporting foot frames (110), and the bottoms of the supporting foot frames (110) are fixedly provided with suckers (120).