CN220460492U - Immune cell culture bag mixing mechanism - Google Patents
Immune cell culture bag mixing mechanism Download PDFInfo
- Publication number
- CN220460492U CN220460492U CN202321984214.8U CN202321984214U CN220460492U CN 220460492 U CN220460492 U CN 220460492U CN 202321984214 U CN202321984214 U CN 202321984214U CN 220460492 U CN220460492 U CN 220460492U
- Authority
- CN
- China
- Prior art keywords
- fixedly connected
- incubator
- immune cell
- culture bag
- cell culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002156 mixing Methods 0.000 title claims abstract description 57
- 210000002865 immune cell Anatomy 0.000 title claims abstract description 35
- 230000007246 mechanism Effects 0.000 title claims abstract description 30
- 238000004113 cell culture Methods 0.000 title claims abstract description 29
- 238000004062 sedimentation Methods 0.000 claims abstract description 10
- 244000309464 bull Species 0.000 claims abstract description 8
- 238000009434 installation Methods 0.000 claims description 11
- 230000006835 compression Effects 0.000 claims description 7
- 238000007906 compression Methods 0.000 claims description 7
- 238000001125 extrusion Methods 0.000 claims description 7
- 230000006978 adaptation Effects 0.000 claims description 5
- 230000000694 effects Effects 0.000 abstract description 12
- 239000011435 rock Substances 0.000 abstract description 5
- 230000010261 cell growth Effects 0.000 abstract description 4
- 238000000034 method Methods 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 6
- 230000008569 process Effects 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- FGRBYDKOBBBPOI-UHFFFAOYSA-N 10,10-dioxo-2-[4-(N-phenylanilino)phenyl]thioxanthen-9-one Chemical compound O=C1c2ccccc2S(=O)(=O)c2ccc(cc12)-c1ccc(cc1)N(c1ccccc1)c1ccccc1 FGRBYDKOBBBPOI-UHFFFAOYSA-N 0.000 description 1
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 210000001539 phagocyte Anatomy 0.000 description 1
- 239000004017 serum-free culture medium Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Abstract
The utility model relates to an immune cell culture bag mixing mechanism which comprises an incubator, wherein a sedimentation treatment mechanism is arranged in the incubator, the sedimentation treatment mechanism comprises a swinging rod rotatably connected to the inner top wall of the incubator, the bottom of the swinging rod is fixedly connected with a mixing box which is in sliding connection with the incubator, the inside of the mixing box is slidably connected with a mounting frame, the right side of the incubator is fixedly connected with a motor, the output end of the motor is fixedly connected with a driving wheel, and the outer side of the swinging rod is sleeved with a sliding sleeve rotatably connected with the driving wheel. This immune cell culture bag mixing mechanism, usable motor make the drive wheel rotate for the sliding sleeve is rotated around the drive wheel axis under the effect of connecting the bull stick, thereby drives the reciprocal swing of swinging arms, makes the installing frame in the mixing case and the culture bag of wherein produce and rocks, makes the immune cell in the culture bag carry out the homogeneous mixing, avoids appearing the phenomenon of subsidence, has guaranteed immune cell's expansion effect.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to an immune cell culture bag mixing mechanism.
Background
Immune cells refer to cells involved in or associated with immune responses, commonly known as leukocytes, including congenital lymphocytes, various phagocytes, and the like, and lymphocytes capable of recognizing antigens and generating specific immune responses, and the like, and can be divided into a plurality of types, and play an important role in various immune cells in human bodies, so that the immune cells have very wide application in cell and gene therapy, and serum-free culture medium is a very critical link in immune cell culture.
Cell culture refers to a method for simulating in-vivo environment (asepsis, proper temperature, pH value, certain nutrition condition and the like) in vitro to enable the cells to survive, grow, reproduce and maintain main structures and functions, and a culture bag is a culture container, is a disposable sealed bag, and is used for uniformly mixing culture solution in the cell growth environment at intervals in the process of cell culture, and fully mixing medicines, cells and the culture solution with the cells so as to prevent the medicines or substances in the culture solution from fully contacting the cells due to long time, so that experimental data deviation can be caused.
Please refer to an immune cell culture bag mixing device with the publication number of CN213913465U, through U-shaped supporting plate, pivot, first bearing frame, transmission shaft, first bevel gear, second bevel gear, spacing slider, reciprocal lead screw, rack, reciprocal slider and motor that are equipped with, can drive to place the immune cell culture bag of incasement portion and rock to can make the immune cell homogeneous mixing of culture bag portion, avoid appearing the phenomenon of subsidence.
However, when the above embodiment is used, the first bevel gear needs to utilize the second bevel gear to drive the placement box to rotate, and the placement box is larger in weight under the condition of placing the culture bag, and the teeth of the first bevel gear and the second bevel gear need to bear larger stress and cannot be used for a long time, and another technical scheme is provided for solving the technical problem aiming at the problem.
Disclosure of Invention
Aiming at the defects of the prior art, the utility model provides an immune cell culture bag mixing mechanism which has the advantages of simple structure, convenient use, stable culture bag installation, high swinging efficiency of a mixing box, good anti-sedimentation effect and the like, and solves the problems that immune cells can be settled to the bottom of the culture bag due to gravity in the existing immune cell culture process, so that immune cell distribution is uneven, expansion of immune cells is restricted to a certain extent, and the existing immune cell culture bag mixing device cannot be used for a long time.
In order to achieve the above purpose, the present utility model provides the following technical solutions: an immune cell culture bag mixing mechanism comprises an incubator, wherein a sedimentation treatment mechanism is arranged in the incubator;
the sedimentation treatment mechanism comprises an inner top wall of the incubator, a swinging rod is rotatably connected to the inner top wall of the incubator, a mixing box which is in sliding connection with the incubator is fixedly connected to the bottom of the swinging rod, a mounting frame is slidably connected to the inside of the mixing box, a motor is fixedly connected to the right side of the incubator, a driving wheel is fixedly connected to the output end of the motor, and a sliding sleeve which is rotatably connected with the driving wheel is sleeved on the outer side of the swinging rod;
the sedimentation treatment mechanism further comprises a fixed plate fixedly connected with the inside of the installation frame, compression springs are fixedly connected to the front side and the rear side of the fixed plate, an extrusion plate is fixedly connected to the other end of each compression spring, a pull rod is movably connected to the bottom of the mixing box, a positioning block is fixedly connected to the top of the pull rod and is clamped with the installation frame, and an extrusion spring fixedly connected with the positioning block and the mixing box is sleeved on the outer side of the pull rod.
Further, limit sliding blocks are fixedly connected to the left side and the right side of the mixing box, and arc grooves which are matched with the limit sliding blocks in a sliding manner are formed in the inner walls of the left side and the right side of the incubator.
Further, the guide plates are fixedly connected to the left side and the right side of the mounting frame, and guide grooves which are in sliding fit with the guide plates are formed in the inner walls of the left side and the right side of the mixing box.
Further, the right side fixedly connected with of sliding sleeve connects the bull stick, the inside of drive wheel is seted up and is connected the spread groove of bull stick looks adaptation.
Further, the inner top wall of the incubator is fixedly connected with a fixed rod, and a movable hole corresponding to the fixed rod in position is formed in the swinging rod.
Further, the front of the installation frame is fixedly connected with a handle, and the bottom of the installation frame is provided with a positioning groove corresponding to the position of the positioning block.
Compared with the prior art, the technical scheme of the application has the following beneficial effects:
this immune cell culture bag mixing mechanism, through the subsidence treatment mechanism that sets up, usable motor makes the drive wheel rotate for the sliding sleeve is connecting the effect of bull stick and is rotating around the drive wheel axis, thereby drives the reciprocal swing of swinging arms, makes the installing frame in the mixing case and the culture bag among them produce and rocks, makes the immune cell in the culture bag carry out the homogeneous mixing, avoids appearing the phenomenon of subsidence, has guaranteed immune cell's expansion effect.
Drawings
FIG. 1 is a cross-sectional view of the structure of the present utility model;
FIG. 2 is a side view of the structure of the present utility model;
FIG. 3 is a top plan view of the mounting frame of the present utility model;
fig. 4 is a schematic diagram of the structure of fig. 1 a according to the present utility model.
In the figure: 1. an incubator; 2. a motor; 3. a driving wheel; 4. connecting a rotating rod; 5. a sliding sleeve; 6. a swinging rod; 7. a fixed rod; 8. a mixing box; 9. a mounting frame; 10. a fixing plate; 11. a compression spring; 12. an extrusion plate; 13. a pull rod; 14. a positioning block; 15. the spring is pressed.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1-4, an immune cell culture bag mixing mechanism in this embodiment includes an incubator 1, and a sedimentation treatment mechanism is disposed inside the incubator 1.
In this embodiment, subside processing mechanism includes that the interior roof of incubator 1 rotates and is connected with swinging arms 6, the interior roof fixedly connected with dead lever 7 of incubator 1, the inside of swinging arms 6 has been seted up with the corresponding movable hole in dead lever 7 position, it is convenient to utilize dead lever 7 to carry out spacingly to the top of swinging arms 6, make swinging arms 6 only can rotate around the axis of dead lever 7, the bottom fixedly connected with of swinging arms 6 and incubator 1 sliding connection's blending box 8, the equal fixedly connected with spacing slider in the left and right sides of blending box 8, the arc wall with spacing slider slip adaptation has been seted up to the left and right sides inner wall of incubator 1, the movable stability of blending box 8 is improved to the cooperation of conveniently utilizing spacing slider and arc wall.
Wherein, the inside sliding connection of blending case 8 has installing frame 9, the equal fixedly connected with deflector of the left and right sides of installing frame 9, the guide way with deflector slip adaptation is seted up to the inner wall of blending case 8 left and right sides, conveniently utilize deflector and guide way to keep installing frame 9 at the activity of blending incasement 8 stable, the right side fixedly connected with motor 2 of incubator 1, the output fixedly connected with drive wheel 3 of motor 2, the sliding sleeve 5 of rotating with drive wheel 3 to be connected has been cup jointed in the outside of swinging arms 6, the right side fixedly connected with connecting rod 4 of sliding sleeve 5, the spread groove with connecting rod 4 looks adaptation has been seted up to the inside of drive wheel 3, conveniently utilize connecting rod 4 and drive wheel 3 to realize the rotation of sliding sleeve 5 and drive wheel 3 and be connected.
In this embodiment, subside the fixed plate 10 of processing mechanism still including the inside fixed connection of installing frame 9, the front and back both sides of fixed plate 10 are all fixedly connected with compression spring 11, compression spring 11's the other end fixedly connected with stripper plate 12, the bottom swing joint of blending case 8 has pull rod 13, the top fixedly connected with of pull rod 13 and the locating piece 14 of installing frame 9 joint, the front fixedly connected with handle of installing frame 9, the constant head tank corresponding with locating piece 14 position has been seted up to the bottom of installing frame 9, conveniently utilize the cooperation of locating piece 14 and constant head tank to install installing frame 9 fixed mounting in blending case 8, the outside of pull rod 13 has cup jointed the extrusion spring 15 with locating piece 14 and blending case 8 fixed connection.
The working principle of the embodiment is as follows:
this immune cell culture bag mixing mechanism, through the subsidence treatment mechanism that sets up, available motor 2 makes drive wheel 3 rotate for sliding sleeve 5 is connecting the effect of bull stick 4 and is rotating around drive wheel 3 axis, thereby drive swinging arms 6 reciprocal swing, make the mount frame 9 in the mixing case 8 and the culture bag wherein produce and rock, make the immune cell in the culture bag carry out the homogeneous mixing, avoid appearing the phenomenon of subsidence, guaranteed immune cell's expansion effect.
The working principle of the embodiment is as follows:
this immune cell culture bag mixing mechanism, when using, can place the cell culture bag in the installation frame 9 one side of stripper plate 12, make stripper plate 12 extrude fixedly to the cell culture bag under the effect of extrusion spring 15, prevent that the cell culture bag from taking place to drop and the condition of collision, afterwards insert mixing box 8 with installation frame 9, and make locating piece 14 and installation frame 9 joint under the effect of extrusion spring 15, thereby fix installation frame 9 in mixing box 8, then can utilize motor 2's driving effect, make drive wheel 3 utilize connecting bull stick 4 drive sliding sleeve 5 to rotate, make sliding sleeve 5 drive swinging arms 6 control the activity, cooperate the limiting displacement of dead lever 7 to swinging arms 6, can make swinging arms 6 bottom drive mixing box 8 reciprocal swing, make the installation frame 9 in mixing box 8 and cell culture bag therein produce and rock.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. Immune cell culture bag mixing mechanism, including incubator (1), its characterized in that: a sedimentation treatment mechanism is arranged in the incubator (1);
the sedimentation treatment mechanism comprises an inner top wall of an incubator (1), a swinging rod (6) is rotationally connected to the inner top wall of the incubator (1), a mixing box (8) which is in sliding connection with the incubator (1) is fixedly connected to the bottom of the swinging rod (6), a mounting frame (9) is slidably connected to the inside of the mixing box (8), a motor (2) is fixedly connected to the right side of the incubator (1), a driving wheel (3) is fixedly connected to the output end of the motor (2), and a sliding sleeve (5) which is rotationally connected with the driving wheel (3) is sleeved on the outer side of the swinging rod (6);
sedimentation processing mechanism still includes fixed plate (10) of the inside fixed connection of installing frame (9), both sides all fixedly connected with compression spring (11) around fixed plate (10), compression spring's the other end fixedly connected with stripper plate (12), the bottom swing joint of blending case (8) has pull rod (13), the top fixedly connected with of pull rod (13) is with locating piece (14) of installing frame (9) joint, extrusion spring (15) with locating piece (14) and blending case (8) fixed connection have been cup jointed in the outside of pull rod (13).
2. The immune cell culture bag mixing mechanism of claim 1 wherein: limiting sliding blocks are fixedly connected to the left side and the right side of the mixing box (8), and arc-shaped grooves which are matched with the limiting sliding blocks in a sliding manner are formed in the inner walls of the left side and the right side of the incubator (1).
3. The immune cell culture bag mixing mechanism of claim 1 wherein: the left side and the right side of the installation frame (9) are fixedly connected with guide plates, and guide grooves which are matched with the guide plates in a sliding manner are formed in the inner walls of the left side and the right side of the mixing box (8).
4. The immune cell culture bag mixing mechanism of claim 1 wherein: the right side fixedly connected with of sliding sleeve (5) connects bull stick (4), the spread groove with connecting bull stick (4) looks adaptation has been seted up to the inside of drive wheel (3).
5. The immune cell culture bag mixing mechanism of claim 1 wherein: the inner top wall of the incubator (1) is fixedly connected with a fixed rod (7), and a movable hole corresponding to the fixed rod (7) is formed in the swinging rod (6).
6. The immune cell culture bag mixing mechanism of claim 1 wherein: the front of the mounting frame (9) is fixedly connected with a handle, and the bottom of the mounting frame (9) is provided with a positioning groove corresponding to the positioning block (14).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321984214.8U CN220460492U (en) | 2023-07-26 | 2023-07-26 | Immune cell culture bag mixing mechanism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321984214.8U CN220460492U (en) | 2023-07-26 | 2023-07-26 | Immune cell culture bag mixing mechanism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220460492U true CN220460492U (en) | 2024-02-09 |
Family
ID=89777509
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321984214.8U Active CN220460492U (en) | 2023-07-26 | 2023-07-26 | Immune cell culture bag mixing mechanism |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220460492U (en) |
-
2023
- 2023-07-26 CN CN202321984214.8U patent/CN220460492U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN220460492U (en) | Immune cell culture bag mixing mechanism | |
| CN109679848B (en) | Culture device for pluripotent stem cells and application of culture device | |
| CN210117372U (en) | Novel microorganism culture turntable | |
| CN212324953U (en) | Seedling conveying mechanism in garden seedling planting machine | |
| CN111304063A (en) | Tumor cell culture bottle preprocessing device | |
| CN213473595U (en) | Automatic preparation partial shipment device of culture medium | |
| CN113150986A (en) | Pathology have culture dish that can dismantle heating structure | |
| CN115109702A (en) | Culture apparatus of cardiac muscle cell | |
| CN210134098U (en) | Bacteria culture securing device | |
| CN219117428U (en) | Rack for microbial cell culture | |
| CN211457991U (en) | Fertilizer distributor convenient to height-adjusting | |
| CN220503046U (en) | Constant temperature incubator convenient to get material | |
| CN220265145U (en) | Movable lifting platform matched with artificial climate box | |
| CN220012668U (en) | Test tube infant incubator | |
| CN219079550U (en) | Adjustable car-T cell culture dish frame | |
| CN217116878U (en) | Energy-concerving and environment-protective type agricultural seeder | |
| CN218277974U (en) | Plant breeding device | |
| CN212894675U (en) | Constant-temperature medical viable bacteria incubator | |
| CN220703715U (en) | Cell culture device | |
| CN218666003U (en) | Automatic add microbial cultivation device of culture medium | |
| CN216853279U (en) | Cultivation area adjustable hickory chick cultivation device | |
| CN113980806B (en) | Vessel device for cell culture growth | |
| CN217536030U (en) | Carbon dioxide incubator with automatic turntable structure | |
| CN217149205U (en) | Novel multipurpose culture medium bottle for storing culture medium | |
| CN213624184U (en) | Breeding device for aspergillus niger high-yield beta-glucosidase strains |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |