CN113973712B - Plant tissue culture device - Google Patents
Plant tissue culture device Download PDFInfo
- Publication number
- CN113973712B CN113973712B CN202111246056.1A CN202111246056A CN113973712B CN 113973712 B CN113973712 B CN 113973712B CN 202111246056 A CN202111246056 A CN 202111246056A CN 113973712 B CN113973712 B CN 113973712B
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- mounting
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- belt pulley
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- 238000004161 plant tissue culture Methods 0.000 title claims abstract description 15
- 230000007246 mechanism Effects 0.000 claims abstract description 17
- 230000005540 biological transmission Effects 0.000 claims description 18
- 238000007789 sealing Methods 0.000 abstract description 5
- 239000001963 growth medium Substances 0.000 description 17
- 238000000034 method Methods 0.000 description 13
- 230000008569 process Effects 0.000 description 8
- 230000009471 action Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229930195732 phytohormone Natural products 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the technical field of plant tissue culture, and particularly relates to a plant tissue culture device, which comprises a culture box and a culture dish, wherein an upper box cover and a lower box cover are arranged on the culture box, a support frame is arranged on the culture box, a guide frame is arranged on the upper box cover, a stripping frame is arranged in the culture dish, a mounting cavity, a mounting hole and a plurality of equally-spaced mounting and dismounting grooves are formed in the lower box cover, a plurality of positioning seats which are respectively matched with the mounting and dismounting grooves are arranged on the lower box cover, a reserved groove is formed in the positioning seats, a mounting and dismounting mechanism is rotatably arranged in the mounting hole, the mounting and dismounting mechanism comprises a base ring rotatably arranged in the mounting hole, a sealing cover is arranged at the bottom of the base ring, and a first mounting and dismounting plate and a second mounting and dismounting plate are arranged on the base ring.
Description
Technical Field
The invention belongs to the technical field of plant tissue culture, and particularly relates to a plant tissue culture device.
Background
Tissue culture of plants, also called in vitro culture, refers to a technique of separating desired tissues, organs or cells, protoplasts, etc. from plant bodies, culturing by inoculating them under aseptic conditions on a medium containing various nutrients and phytohormones to obtain regenerated whole plants or to produce other products of economic value.
In the culture process, the culture medium is required to be replaced in order to ensure the normal growth of the culture medium, and the culture medium filled with the culture medium is required to be replaced after being taken out from the culture box when the culture medium is replaced. Because the difference between the internal environment and the external environment of the incubator is large, the culture medium is easy to pollute or otherwise physically damage in the process of replacing the culture medium, and the culture effect is affected.
Disclosure of Invention
Based on the problems mentioned in the background art, the invention provides a plant tissue culture device, which is used for solving the problems that the culture body is easy to cause pollution or other physical damages when leaving an incubator and influencing the culture effect when the culture dish is replaced with the current plant tissue culture.
The technical scheme adopted by the invention is as follows:
the plant tissue culture device comprises an incubator and a culture dish, wherein an upper case cover and a lower case cover are arranged on the incubator, a support frame is arranged on the incubator, a guide frame is arranged on the upper case cover, the guide frame comprises a base column and a plurality of obliquely arranged sliding rails, guide grooves are respectively formed in the sliding rails, a stripping frame is arranged in the culture dish, the stripping frame comprises a fixedly connected net rack, a connecting rod and a guide plate, the connecting rod corresponds to the guide grooves, a mounting cavity, a mounting hole and a plurality of equidistant mounting and dismounting grooves are formed in the lower case cover, a plurality of positioning seats which are respectively matched with the mounting and dismounting grooves are arranged on the lower case cover, a reserved groove is formed in the positioning seat, a mounting and dismounting mechanism is rotatably arranged in the mounting hole, the mounting and dismounting mechanism comprises a base ring rotatably arranged in the mounting hole, a sealing cover is arranged at the bottom of the base ring, the first loading plate and the second loading plate are arranged on the base ring, the first loading plate and the second loading plate are positioned in the mounting cavity, the first loading plate and the second loading plate are respectively provided with a linkage sliding groove and a mounting groove, the mounting grooves are respectively internally provided with a first belt wheel and a linkage wheel, the linkage wheel comprises a mounting shaft, a second belt wheel and a gear, the second belt wheel and the gear of the linkage wheel on the first loading plate are respectively fixedly arranged on the mounting shaft, the second belt wheel of the linkage wheel on the second loading plate is fixedly arranged on the mounting shaft and is connected with the mounting shaft through one-way transmission of a ratchet mechanism, a transmission belt is wound between the first belt wheel and the second belt wheel, the first loading plate and the second loading plate are respectively provided with a first push head and a second push head, the first push head and the second push head are respectively provided with a transmission rod, the transmission rod is respectively provided with a connecting column, the first pushing head and the second pushing head are fixedly connected with two driving belts through connecting columns, an adjusting ring is sleeved on the base ring, a gear ring is arranged on the adjusting ring and meshed with the gear, the first pushing head comprises a pushing head seat and a movable head, a mounting sliding groove is formed in the pushing head seat, a mounting column is arranged in the mounting sliding groove, the movable head is sleeved on the mounting column, a wedge surface is formed in the movable head, and a spring is arranged between the movable head and the mounting sliding groove.
Based on the technical scheme, the invention also improves the following steps:
further, limiting sliding grooves are formed in the first loading and unloading plate and the second loading and unloading plate, guide blocks are arranged on the first pushing head and the second pushing head, and the guide blocks are located in the limiting sliding grooves. The first push head and the second push head are limited by the cooperation of the guide block and the limiting chute, so that the first push head and the second push head are prevented from being swayed in the moving process to influence the pushing of the culture dish.
Further, the first pushing head and the second pushing head are arc-shaped pushing plates matched with the culture dishes. The first pushing head and the second pushing head can be better attached to the culture dish, and the culture dish can be made to travel along a straight line in the pushing process.
Further, a limiting block is arranged in the linkage sliding groove on the second loading and unloading plate, and the inner wall of the second push head is coplanar with the inner wall of the base ring when the transmission rod on the second push head is in contact with the limiting block. The transmission rod on the second push head is limited through the limiting block to achieve the purpose of limiting the second push head, so that the culture dish corresponds to the base ring when the first push head drives the culture dish to move to the limit position, and the culture dish is convenient to replace.
Further, the ports of the limiting chute and the linkage chute on the first loading and unloading plate are larger than the ports of the limiting chute and the linkage chute on the second loading and unloading plate. The guide block and the transmission rod on the second push head can more easily enter the limiting chute and the linkage chute on the first loading and unloading plate.
Further, the bottom of culture dish is equipped with the guide holder, the diameter of guide holder and loading and unloading groove's width size phase-match. The culture dish is prevented from deflecting in the moving process by the cooperation of the guide seat and the loading and unloading groove.
Further, the open ends of the guide grooves are provided with guide openings. The connecting rod can be clamped into the guide groove more easily.
Further, a collecting tray is installed at the lower part of the installation hole. The culture liquid which drops when the culture medium is replaced can be collected through the collecting tray.
The invention has the beneficial effects that:
through setting up the leading truck and placing the rack that peels off in the culture dish, place the culture medium in the rack top of peeling off and cultivate, drive the culture dish through handling mechanism when need changing the culture dish and remove, it slides along the leading truck and rises to break away from the culture dish bottom and take out the culture medium from the culture solution to peel off the frame at the in-process that removes, take out the culture dish from the base ring after changing new culture dish, rethread handling mechanism drives new culture dish and returns to the position, peel off the frame whereabouts and send the culture medium into new culture solution in the in-process that the culture dish returns to the position and can accomplish the change of culture medium, the in-process culture medium that changes is located the incubator all the time, operating personnel need not to contact with the culture medium simultaneously, avoid influencing the culture effect.
Drawings
The invention can be further illustrated by means of non-limiting examples given in the accompanying drawings;
FIG. 1 is a schematic diagram of a plant tissue culture apparatus according to an embodiment of the present invention;
FIG. 2 is a schematic view of a longitudinal section of a plant tissue culture apparatus according to an embodiment of the present invention;
FIG. 3 is a schematic view of a guide frame according to an embodiment of the present invention;
FIG. 4 is a schematic view of a longitudinal section of an incubator according to an embodiment of the present invention;
FIG. 5 is a schematic view of a loading and unloading mechanism according to an embodiment of the present invention;
FIG. 6 is an enlarged schematic view of the structure shown at A in FIG. 5;
FIG. 7 is a schematic view of a longitudinal section of a loading and unloading mechanism according to an embodiment of the present invention;
FIG. 8 is an enlarged schematic view of the structure shown at B in FIG. 7;
FIG. 9 is a schematic diagram of a first push head according to an embodiment of the present invention;
FIG. 10 is a schematic view of a linkage wheel according to an embodiment of the present invention;
FIG. 11 is a schematic view of a loading and unloading mechanism for replacing a culture dish according to an embodiment of the present invention;
the main reference numerals are as follows:
incubator 1, support frame 11, upper case lid 12, collection tray 13, guide frame 2, base post 21, slide rail 22, guide groove 23, guide port 24, culture dish 4, lower case lid 3, mounting hole 31, mounting chamber 32, mounting and dismounting groove 33, positioning seat 34, reserve tank 35, culture medium 4, guide seat 41, net frame 51, connecting rod 52, guide plate 53, mounting and dismounting mechanism 6, seal plate 60, base ring 61, first mounting and dismounting plate 621, second mounting and dismounting plate 622, limit chute 631, linkage chute 632, mounting groove 633, first pulley 64, linkage wheel 65, mounting shaft 651, second pulley 652, gear 653, belt 66, adjusting ring 67, ring gear 671, first pusher 68, guide block 681, transmission rod 682, connecting post 683, pusher seat 684, mounting chute 685, movable head 686, wedge surface 687, mounting post 688, second pusher 69, spring 689, and limit stopper 7.
Detailed Description
In order that those skilled in the art will better understand the present invention, the following technical scheme of the present invention will be further described with reference to the accompanying drawings and examples.
As shown in fig. 1 to 11, a plant tissue culture device comprises a culture box 1 and a culture dish 4, wherein an upper box cover 12 and a lower box cover 3 are arranged on the culture box 1, a support frame 11 is arranged on the culture box 1, a guide frame 2 is arranged on the upper box cover 12, the guide frame 2 comprises a base column 21 and a plurality of obliquely arranged slide rails 22, guide grooves 23 are formed in the slide rails 22, a stripping frame is arranged in the culture dish 4 and comprises a fixedly connected net rack 51, a connecting rod 52 and a guide plate 53, when tissue culture is carried out, the stripping frame is placed in the culture dish, then culture solution is injected into the culture dish, a culture body is placed above the net rack 51 for culture, and the culture body can be taken out by the connecting rod 52; the connecting rod 52 corresponds to the guide groove 23, the lower case cover 3 is provided with a mounting cavity 32, a mounting hole 31 and a plurality of equidistant mounting and dismounting grooves 33, the lower case cover 3 is provided with a plurality of positioning seats 34 which are respectively matched with the mounting and dismounting grooves 33, the positioning seats 34 are provided with reserved grooves 35, and the culture medium 4 can be corrected through the positioning seats 34;
the mounting hole 31 is rotatably provided with the loading and unloading mechanism 6, the loading and unloading mechanism 6 comprises a base ring 61 rotatably arranged in the mounting hole 31, wherein the base ring 61 is penetrated in the mounting hole 31, the top end of the base ring 61 is fixedly provided with an anti-falling hanging plate, the hanging plate is positioned at the upper part of the lower box cover 3, the base ring 61 is prevented from falling from the mounting hole 31 under the action of the anti-falling hanging plate, the bottom of the base ring 61 is provided with a sealing cover 60, the sealing cover 60 is opened when the culture medium 4 needs to be replaced, and the culture dish is loaded and unloaded through the inside of the base ring 61; the base ring 61 is provided with a first loading plate 621 and a second loading plate 622, the first loading plate 621 and the second loading plate 622 are located in the mounting cavity 32, a linkage sliding groove 632 and a mounting groove 633 are formed in the first loading plate 621 and the second loading plate 622, a first belt pulley 64 and a linkage wheel 65 are mounted in the mounting groove 633, the linkage wheel 65 comprises a mounting shaft 651, a second belt pulley 652 and a gear 653, the second belt pulley 652 and the gear 653 of the linkage wheel 65 on the first loading plate 621 are fixedly mounted on the mounting shaft 651, the second belt pulley 652 of the linkage wheel 65 on the second loading plate 622 is fixedly mounted on the mounting shaft 651, a gear 653 is in unidirectional transmission connection with the mounting shaft 651 through a ratchet mechanism, a driving belt 66 is wound between the first belt pulley 64 and the second belt pulley 652, a first push head 68 and a second push head 69 are mounted on the first belt pulley 621 and the second belt 622 respectively, a driving rod 682 is mounted on the first push head 68 and the second push head 69, a connecting post 683 is mounted on the driving rod 682, the second push head 68 and the second push head 686 are mounted on the second push head 68 are mounted on the first push head 68 and the second push head 686, a movable sleeve 686 is mounted on the mounting sleeve 686 through the driving sleeve 686, a movable sleeve 686 is mounted on the driving sleeve 686, and the driving sleeve 686 is mounted on the mounting sleeve 686.
Specifically, in order to prevent the first push head 68 and the second push head 69 from shaking and deflecting during the moving process to affect the pushing of the culture dish 4, the first loading plate 621 and the second loading plate 622 are provided with limiting sliding grooves 631, the first push head 68 and the second push head 69 are provided with guide blocks 681, the guide blocks 681 are positioned in the limiting sliding grooves 631, and the movement of the first push head 68 and the second push head 69 is limited by the cooperation of the guide blocks 681 and the limiting sliding grooves 631.
Specifically, since the outer wall of the culture dish 4 is a curved surface, in order to make the first push head 68 and the second push head 69 better fit with the culture dish 4, the first push head 68 and the second push head 69 are arc-shaped push plates matched with the culture dish 4, and the culture dish 4 can travel along a straight line in the pushing process.
Specifically, the limiting block 7 is installed in the linkage chute 632 on the second loading and unloading plate 622, when the transmission rod 682 on the second push head 69 contacts with the limiting block 7, the inner wall of the second push head 69 is coplanar with the inner wall of the base ring 61, and the purpose of limiting the second push head 69 is achieved by limiting the transmission rod 682 on the second push head 69 through the limiting block 7, so that the culture dish 4 corresponds to the base ring 61 when the first push head 68 drives the culture dish 4 to move to the limit position, and the culture dish 4 is convenient to replace.
Specifically, in order to make it easier for the guide block 681 and the transmission rod 682 on the second pusher 69 to enter the limit slide slot 631 and the link slide slot 632 on the first loading and unloading plate 621, the ports of the limit slide slot 631 and the link slide slot 632 on the first loading and unloading plate 621 are larger than the ports of the limit slide slot 631 and the link slide slot 632 on the second loading and unloading plate 622.
Specifically, in order to prevent the culture dish 4 from being deflected during the movement, a guide seat 41 is provided at the bottom end of the culture dish 4, the diameter of the guide seat 41 is matched with the width of the loading and unloading slot 33, and the culture dish 4 is restricted by the cooperation of the guide seat 41 and the loading and unloading slot 33.
Specifically, in order to make it easier for the link 52 to be caught in the guide groove 23, the guide grooves 23 are each provided with a guide opening 24 at the open end thereof.
Specifically, the collecting tray 13 is mounted to the lower portion of the mounting hole 31. The culture liquid dropped when the medium 4 is replaced can be collected by the collection tray 13.
When the culture dish 4 is replaced, the rotary base ring 61 rotates the first loading and unloading plate 621 to the position of the corresponding loading and unloading groove 33 of the culture dish 4 to be replaced, and when the first loading and unloading plate 621 moves, the movable head 686 of the first push head 68 contacts with the top end of the mounting cavity 32 to shrink until the first push head moves to the proper position, and then the first push head springs up to one side of the culture dish 4 under the action of the spring 689; then, the rotation adjusting ring 67 drives the gear 653 to rotate to drive the driving belt 66 to rotate to drive the first pushing head 68 to move to drive the culture dish 4 to move, the stripping frame slides along the guide frame 2 and ascends to be separated from the bottom of the culture dish 4 in the moving process to carry out the culture body from the culture solution, the second pushing head 69 is not moved at the moment because the gear 653 of the second pushing head 69 is in unidirectional transmission connection with the mounting shaft 651 through the ratchet mechanism, the culture dish 4 cannot move continuously in the self-falling room ring 61 after being contacted with the second pushing head 69, the sealing plate 60 is opened at the moment, the culture dish 4 is taken out and put into a new culture dish 4, and the stripping frame enters the new culture dish 4 at the moment but is still in a separation state with the culture solution in the culture dish 4; then the adjusting ring 67 is rotated, the adjusting ring 67 drives the driving belt 66 on the first pushing head 68 and the second pushing head 69 to rotate through the gear 653, the first pushing head 68 and the second pushing head 69 move simultaneously, the new culture dish 4 is sent to the original culture dish 4 under the action of the second pushing head 69, and the stripping frame returns simultaneously to enable the culture body to enter the culture solution again to finish the replacement of the culture dish.
The plant tissue culture device provided by the invention is described in detail. The description of the specific embodiments is only intended to aid in understanding the method of the present invention and its core ideas. It should be noted that it will be apparent to those skilled in the art that various modifications and adaptations of the invention can be made without departing from the principles of the invention and these modifications and adaptations are intended to be within the scope of the invention as defined in the following claims.
Claims (2)
1. Plant tissue culture device, including incubator (1) and culture dish (4), be equipped with case lid (12) and lower case lid (3) on incubator (1), install support frame (11), its characterized in that on incubator (1): the upper box cover (12) is provided with a guide frame (2), the guide frame (2) comprises a base column (21) and a plurality of slide rails (22) which are obliquely arranged, the slide rails (22) are provided with guide grooves (23), a stripping frame is arranged in the culture dish (4), the stripping frame comprises a fixedly connected net frame (51), a connecting rod (52) and a guide plate (53), the connecting rod (52) corresponds to the guide groove (23), the lower box cover (3) is provided with a mounting cavity (32), a mounting hole (31) and a plurality of equidistant mounting and dismounting grooves (33), the lower box cover (3) is provided with a plurality of positioning seats (34) which are respectively matched with the mounting and dismounting grooves (33), the positioning seats (34) are provided with a reserved groove (35), the mounting and dismounting mechanism (6) is rotationally arranged in the mounting hole (31), the bottom of the base ring (61) is provided with a mounting hole (61), the first mounting and dismounting plates (621) are arranged in the first mounting and dismounting plates (622), the first loading and unloading plate (621) and the second loading and unloading plate (622) are respectively provided with a linkage sliding groove (632) and a mounting groove (633), the mounting groove (633) is internally provided with a first belt pulley (64) and a linkage wheel (65), the linkage wheel (65) comprises a mounting shaft (651), a second belt pulley (652) and a gear (653), the second belt pulley (652) and the gear (653) of the linkage wheel (65) on the first loading and unloading plate (621) are respectively and fixedly arranged on the mounting shaft (651), the second belt pulley (652) of the linkage wheel (65) on the second loading and unloading plate (622) is fixedly arranged on the mounting shaft (651), the gear (653) is connected with the mounting shaft (651) through a one-way transmission mechanism, a transmission belt (66) is wound between the first belt pulley (64) and the second belt pulley (652), the first belt pulley (621) and the second belt pulley (69) are respectively arranged on the first loading and unloading plate (621), the second belt pulley (652) and the second belt pulley (69) are respectively fixedly arranged on the mounting shaft (651), the first belt pulley (68) and the second belt pulley (69) are respectively connected with the transmission rod (68) through the transmission rod (68), the novel multifunctional electric hair cutter is characterized in that an adjusting ring (67) is sleeved on the base ring (61), a gear ring (671) is arranged on the adjusting ring (67), the gear ring (671) is meshed with a gear (653), the first push head (68) comprises a push head seat (684) and a movable head (686), a mounting chute (685) is formed in the push head seat (684), a mounting column (688) is arranged in the mounting chute (685), the movable head (686) is sleeved on the mounting column (688), a wedge surface (687) is formed in the movable head (686), and a spring (689) is arranged between the movable head (686) and the mounting chute (685); limiting sliding grooves (631) are formed in the first loading and unloading plate (621) and the second loading and unloading plate (622), guide blocks (681) are arranged on the first pushing head (68) and the second pushing head (69), and the guide blocks (681) are located in the limiting sliding grooves (631); the first pushing head (68) and the second pushing head (69) are arc-shaped pushing plates matched with the culture dish (4); a limiting block (7) is arranged in the linkage sliding chute (632) on the second loading and unloading plate (622), and when the transmission rod (682) on the second push head (69) is in contact with the limiting block (7), the inner wall of the second push head (69) is coplanar with the inner wall of the base ring (61); the ports of the limiting chute (631) and the linkage chute (632) on the first loading and unloading plate (621) are larger than the ports of the limiting chute (631) and the linkage chute (632) on the second loading and unloading plate (622); the bottom end of the culture dish (4) is provided with a guide seat (41), and the diameter of the guide seat (41) is matched with the width of the loading and unloading groove (33); the open ends of the guide grooves (23) are provided with guide openings (24).
2. A plant tissue culture apparatus according to claim 1, wherein: a collecting tray (13) is arranged at the lower part of the mounting hole (31).
Priority Applications (1)
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CN202111246056.1A CN113973712B (en) | 2021-10-26 | 2021-10-26 | Plant tissue culture device |
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CN202111246056.1A CN113973712B (en) | 2021-10-26 | 2021-10-26 | Plant tissue culture device |
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CN113973712A CN113973712A (en) | 2022-01-28 |
CN113973712B true CN113973712B (en) | 2023-10-24 |
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CN115067211A (en) * | 2022-06-24 | 2022-09-20 | 唐山师范学院 | Multifunctional temperature control device for microspore culture |
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CN109331892A (en) * | 2018-10-16 | 2019-02-15 | 程忆琴 | A kind of biologic test box of overturning-preventing |
CN112970586A (en) * | 2021-04-21 | 2021-06-18 | 广州煜景科技服务有限公司 | Plant tissue culture device with switching function |
CN113073049A (en) * | 2021-04-21 | 2021-07-06 | 河南亚都实业有限公司 | Bioreactor for artificial skin tissue culture |
CN214015421U (en) * | 2020-12-25 | 2021-08-24 | 山西农业大学 | Hair is built careless tissue culture seedling culture apparatus that takes root |
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2021
- 2021-10-26 CN CN202111246056.1A patent/CN113973712B/en active Active
Patent Citations (4)
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CN109331892A (en) * | 2018-10-16 | 2019-02-15 | 程忆琴 | A kind of biologic test box of overturning-preventing |
CN214015421U (en) * | 2020-12-25 | 2021-08-24 | 山西农业大学 | Hair is built careless tissue culture seedling culture apparatus that takes root |
CN112970586A (en) * | 2021-04-21 | 2021-06-18 | 广州煜景科技服务有限公司 | Plant tissue culture device with switching function |
CN113073049A (en) * | 2021-04-21 | 2021-07-06 | 河南亚都实业有限公司 | Bioreactor for artificial skin tissue culture |
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