CN112970586A - Plant tissue culture device with switching function - Google Patents

Plant tissue culture device with switching function Download PDF

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Publication number
CN112970586A
CN112970586A CN202110428113.1A CN202110428113A CN112970586A CN 112970586 A CN112970586 A CN 112970586A CN 202110428113 A CN202110428113 A CN 202110428113A CN 112970586 A CN112970586 A CN 112970586A
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China
Prior art keywords
unit
culture dish
fixedly connected
culture
box body
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Withdrawn
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CN202110428113.1A
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Chinese (zh)
Inventor
林钘权
梅嘉华
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Guangzhou Yujing Technology Service Co ltd
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Guangzhou Yujing Technology Service Co ltd
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Priority to CN202110428113.1A priority Critical patent/CN112970586A/en
Publication of CN112970586A publication Critical patent/CN112970586A/en
Withdrawn legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model provides a plant tissue culture device with switch function, includes the box, and control terminal is installed to the box lateral wall, be provided with the centre gripping unit of centre gripping plant tissue, the culture dish transmission unit of transmission culture dish in the box, snatch the culture dish snatch the unit, control centre gripping unit and the lifting unit who snatchs the unit and go up and down, the memory cell of storage culture medium. The continuous transfer of plant tissues in a plurality of culture media can be realized through the lifting unit, the browning phenomenon is effectively inhibited, the survival rate of the plant tissues is increased, one or more culture dishes can be selectively replaced without being replaced all at once, and the material cost is reduced.

Description

Plant tissue culture device with switching function
Technical Field
The invention relates to the field of plant tissue culture, in particular to a plant tissue culture device with a switching function.
Background
Tissue culture of plants, also called in vitro culture, refers to a technique of separating desired tissues, organs or cells, protoplasts, etc. from plant bodies, and inoculating them on a culture medium containing various nutrients and phytohormones under aseptic conditions for culture to obtain regenerated whole plants or produce other products having economic value by aseptic manipulation.
The culture method comprises a solid culture method: the agar solidified culture medium is used for culturing plants, and has the advantages that the equipment is simple and easy to operate, but the nutrient distribution is uneven, the growth speed is not balanced, and the browning and poisoning phenomena are easy to occur; liquid culture method: that is, the culture method without adding a strengthening agent to the culture medium, but the oxygen content in the liquid is small, so that the supply of oxygen is usually ensured by stirring or shaking the culture medium, and the distribution of the culture medium is uniform, which is favorable for the growth of plants.
The browning phenomenon refers to the phenomenon that when the cell membrane structure of a culture is changed or damaged in the process of plant tissue culture, after oxidase and a substrate are combined, quinone substances are generated under the oxidation action, and the culture and a culture medium are brown, so that the growth and differentiation of the tissue culture are influenced, and even the culture is dead. The method for preventing browning comprises selecting a variety which is not easy to brown, changing the formula of a culture medium, improving the culture environment and the illumination condition, and continuously transferring;
wherein the continuous transfer means that the material which is easy to brown is cultured for 2-24 hours at intervals and then transferred to a new culture medium, so that the browning phenomenon can be controlled or greatly reduced after 7-10 days of continuous transfer treatment.
There is a need for a plant tissue culture apparatus that reduces the browning of plant tissue.
Disclosure of Invention
The invention aims to provide a plant tissue culture device with a switching function.
The invention aims to realize the technical scheme, which comprises a box body, wherein a control terminal is arranged on the outer side wall of the box body, and a clamping unit for clamping plant tissues, a culture dish transmission unit for transmitting culture dishes, a grabbing unit for grabbing the culture dishes, a lifting unit for controlling the lifting of the clamping unit and the grabbing unit, and a storage unit for storing culture media are arranged in the box body;
the culture dish transmission unit comprises a conveyor belt, the conveyor belt is movably arranged on two side walls of the box body through two transmission shafts and is positioned at the lowest end of the box body, the movement direction of the conveyor belt is the front side, the clamping unit, the grabbing unit and the lifting unit are all positioned above the culture dish transmission unit, the clamping unit and the grabbing unit are arranged at the lifting end of the lifting unit, the clamping unit can lift and extend into a culture dish of the culture dish transmission unit, the storage unit is positioned above the culture dish transmission unit and is positioned at the rear side of the lifting unit, and the outlet end of the storage unit can be communicated with the culture dish transmission unit;
two first grooves capable of containing culture dishes are uniformly formed in the conveying belt along the length direction.
Preferably, the lifting unit comprises a lifting plate, the lifting plate is slidably mounted in a first sliding groove and a second sliding groove on two side walls of the box body through a first sliding block and a second sliding block which are fixedly connected on two sides, the first sliding groove and the second sliding groove are perpendicular to the ground, one end of the first sliding groove is positioned on the bottom surface of the box body, the other end of the first sliding groove is positioned on the top wall of the box body, a first ultraviolet lamp and a plant feeding port are further respectively arranged on two adjacent side walls of one end of the top wall of the box body, the end of the first sliding block, which extends into the first sliding groove, is fixedly connected with a first rack, and the first rack is parallel to the;
the first motor is fixedly connected to the side wall of the box body, an output shaft of the first motor can rotatably extend into the first sliding groove and is fixedly connected with a first gear, and the first gear is meshed with the first rack.
Preferably, the grabbing unit comprises a second motor, a second gear and a first disc are fixedly connected to an output shaft of the second motor from inside to outside in sequence, and a semicircular notch is formed in the edge of the first disc;
the top surface of the lifting plate is fixedly connected with one end of a mounting bracket, the other end of the mounting bracket is fixedly connected with a mounting plate, the mounting plate is perpendicular to the ground, a first mounting table is slidably mounted in a third chute on the mounting plate through a third sliding block on the bottom surface, a second motor is mounted on the first mounting table, an electric telescopic rod parallel to the third chute is further fixedly connected onto the mounting plate, and a push rod of the electric telescopic rod is fixedly connected with the first mounting table;
the bottom surface of the lifting plate is fixedly connected with hollow first cylinders which are uniformly distributed around the circle center of the second gear, the number of the first cylinders is 4, the first cylinders are all positioned above the culture dish, the outer wall of the circumference of one end of each first cylinder, which is far away from the lifting plate, is fixedly connected with a limit ring table, the first cylinders are sleeved with slidable first rings, the first cylinders are also sleeved with springs, and the springs are positioned between the first rings and the limit ring tables;
the limiting ring platform is evenly hinged with one end of a plurality of C-shaped clamping blocks around the circle center, the other ends of the C-shaped clamping blocks can be matched with a culture dish, the back of each C-shaped clamping block is hinged with one end of a first hinge rod, the other ends of the first hinge rods are hinged with a first circular ring, the top surface of the first circular ring is fixedly connected with one end of a first ejector rod, and the other end of the first ejector rod can vertically slide to penetrate through a lifting plate and can be matched with a first circular disc.
Preferably, the clamping unit comprises first rotating rods, the number of the first rotating rods is the same as that of the first cylinders, the first rotating rods are evenly distributed around the circle center of the second gear and rotatably installed on the lifting plate, one end, close to the conveying belt, of each first rotating rod extends out of the hollow first cylinder, a left clamping block and a right clamping block which can be opened and closed are hinged to the outer wall of the circumference of each first rotating rod, the other end of each first rotating rod extends out of the top surface of the lifting plate, and a third gear meshed with the second gear is fixedly connected to the other end of each first.
As preferred, the storage unit is including the locker room, and the locker room rigid coupling just is located the conveyer belt top in the box, locker room bottom face install the intercommunication locker room and can with culture dish complex automatically controlled shower nozzle, second ultraviolet lamp is still installed to automatically controlled shower nozzle one side, the culture medium feed inlet has been seted up to the roof in locker room, third ultraviolet lamp is installed to the inboard roof in locker room.
Preferably, a culture dish inlet is formed in the rear side wall of the box body, a culture dish collecting box is fixedly connected in the box body and located below the front side of the conveyor belt, and a culture dish outlet is formed in the side wall of the culture dish collecting box.
Preferably, a third motor is fixedly connected to the side wall of the box body, and an output shaft of the third motor is fixedly connected with one transmission shaft of the conveyor belt.
Due to the adoption of the technical scheme, the invention has the following advantages:
1. according to the invention, the clamping unit clamps plant roots and stems and inserts the plant roots and stems into the culture dish, when the browning condition occurs, the lifting unit drives the clamping unit to leave the culture dish, the polluted culture medium is poured off by the conveyor belt, and then a new culture dish is conveyed to the position below the lifting unit, so that the continuous switching of the culture dish is realized, the plant browning is avoided, and the survival rate of plant tissues is increased.
2. The switching process of the primary inoculation process and the browning process of the plants is completed by a mechanical device in the box body, so that misoperation of personnel and external bacterial pollution are avoided, and the working efficiency is increased.
3. According to the invention, any first ejector rod can be pressed down through the lifting of the first disc, the first ejector rod drives the first circular ring to descend, the first circular ring drives the C-shaped clamping block to be closed, the grabbing of any culture dish is realized, when the semicircular notch on the first disc is positioned above a certain first ejector rod, the first ejector rod cannot be pressed down, the culture dish at the position cannot be grabbed, when one or more culture dishes are browned and need to be replaced, the position of the semicircular notch is adjusted through rotation, the first disc is lifted, the culture dish which does not need to be replaced is grabbed, the culture dish which needs to be replaced is sent away by the conveyor belt without being completely replaced at one time, and the cost of the culture medium can be saved.
4. The position at semicircle breach place that the first disc of second motor both rotatable switching also can drive the second gear rotation, and the third gear engagement on second gear and the first bull stick, first bull stick rotates, and the grip block can be being held plant tissue at culture dish internal rotation to optional position about, both can be used to mix the culture medium, increases the culture medium and contains oxygen content, also can avoid plant tissue to be motionless in a department all the time, and it is inhomogeneous to absorb the nutrient.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims hereof.
Drawings
The drawings of the present invention are described below.
FIG. 1 is a schematic view of a housing of the present invention.
Fig. 2 is a schematic view of a conveyor belt of the present invention.
Fig. 3 is a schematic view of the lifting unit of the present invention.
Fig. 4 is a schematic view of a second motor of the present invention.
Fig. 5 is a schematic view of a grasping unit of the present invention.
FIG. 6 is a schematic view of a clamping unit according to the present invention.
Fig. 7 is a schematic view of the internal structure of the present invention.
In the figure: 1. a box body; 2. a control terminal; 3. a culture dish; 4. a conveyor belt; 5. a drive shaft; 6. a first groove; 7. a lifting plate; 8. a first slider; 9. a second slider; 10. a first chute; 11. a second chute; 12. a first ultraviolet lamp; 13. a plant feed inlet; 14. a first rack; 15. a first motor; 16. a first gear; 17. a second motor; 18. a second gear; 19. a first disc; 20. a semicircular notch; 21. mounting a bracket; 22. mounting a plate; 23. a first mounting table; 24. a third slider; 25. a third chute; 26. an electric telescopic rod; 27. a push rod; 28. a first cylinder; 29. a limit ring table; 30. a first circular ring; 31. a spring; a C-shaped clamping block; 33. a first hinge rod; 34. a first ejector rod; 35. a first rotating lever; 36. a left clamping block; 37. a right clamping block; 38. a third gear; 39. a storage chamber; 40. an electrically controlled spray head; 41. a second ultraviolet lamp; 42. a culture medium feed port; 43. a third ultraviolet lamp; 44. a culture dish inlet; 45. a culture dish collecting box; 46. an outlet of the culture dish; 47. a third motor.
Detailed Description
The invention is further illustrated by the following figures and examples.
In the description of the embodiments of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience in describing the embodiments of the present invention and simplifying the description, but do not indicate or imply that the referred devices or elements must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the embodiments of the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. In the description of the embodiments of the present invention, it should be noted that, unless explicitly stated or limited otherwise, the terms "connected" and "connected" are to be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. Specific meanings of the above terms in the embodiments of the present invention can be understood in specific cases by those of ordinary skill in the art.
As shown in fig. 1 to 7, a plant tissue culture device with a switching function includes a box 1, a control terminal 2 is installed on an outer side wall of the box 1, and a clamping unit for clamping plant tissue, a culture dish transmission unit for transmitting a culture dish 3, a grabbing unit for grabbing the culture dish 3, a lifting unit for controlling lifting of the clamping unit and the grabbing unit, and a storage unit for storing culture medium are arranged in the box 1;
the culture dish transmission unit comprises a conveyor belt 4, the conveyor belt 4 is movably arranged on two side walls of the box body 1 through two transmission shafts 5 and is positioned at the lowest end of the box body 1, the movement direction of the conveyor belt 4 is the front side, the clamping unit, the grabbing unit and the lifting unit are all positioned above the culture dish transmission unit, the clamping unit and the grabbing unit are arranged at the lifting end of the lifting unit, the clamping unit can lift and extend into a culture dish 3 of the culture dish transmission unit, the storage unit is positioned above the culture dish transmission unit and is positioned at the rear side of the lifting unit, and the outlet end of the storage unit can be communicated with the culture dish transmission unit;
two first grooves 6 capable of placing the culture dish 3 are uniformly formed in the conveying belt 4 along the length direction.
In the embodiment, the control terminal controls the conveyor belt to move, the culture dishes placed on the conveyor belt move to the storage unit to obtain the culture medium, then move to the lower part of the lifting unit to stop, plant roots are clamped on the clamping unit and are not released all the time, the lifting unit drives the clamping unit and the acquiescent opened grabbing unit to descend, the plant roots are inserted into the culture medium to be cultured, when partial plants are browned and one or more culture mediums need to be replaced, the lifting unit drives the clamping unit and the grabbing unit to ascend, the culture dishes needing to be replaced cannot be grabbed, only the plants in the culture dishes are taken away by the clamping unit, the culture dishes not needing to be replaced are grabbed and ascend together with the lifting unit, the culture dishes which are not grabbed are sent away by the conveyor belt, an operator puts the culture dishes of the next batch into the box, and the placing quantity and the placing positions of the culture dishes are the, the batch of culture dishes are conveyed to the lower part of the lifting unit again by the conveyor belt;
the browned culture dish is required to be replaced, the culture dish which is not browned is not required to be replaced, and the cost of the culture medium can be saved.
As shown in fig. 2 to 3, the lifting unit includes a lifting plate 7, the lifting plate 7 is slidably mounted in a first sliding groove 10 and a second sliding groove 11 on two side walls of the box body 1 through a first sliding block 8 and a second sliding block 9 fixedly connected on two sides of the lifting plate 7, the first sliding groove 10 and the second sliding groove 11 are perpendicular to the ground, one end of the first sliding groove 10 and one end of the second sliding groove 11 are located on the bottom surface of the box body 1, the other end of the first sliding groove 10 are located on the top wall of the box body 1, a first ultraviolet lamp 12 and a plant feed port 13 are respectively arranged on two adjacent side walls of one end of the first sliding groove 10 and the second sliding groove 11 located on the top wall of the box body 1, a first rack 14 is fixedly connected to one end of the;
the first motor 15 is fixedly connected to the side wall of the box body 1, an output shaft of the first motor 15 can rotatably extend into the first chute 10 and is fixedly connected with a first gear 16, and the first gear 16 is meshed with the first rack 14.
In the embodiment, the first motor is started, the first gear rotates, the first gear drives the first rack to slide in the first chute to drive the lifting plate to move up and down, when the lifting plate moves to the top end of the first chute, the plant feeding hole can be opened, a plant is clamped on the clamping unit below the lifting plate, the plant feeding hole is closed, and the first ultraviolet lamp is opened for sterilization;
first motor reversal drives the lifter plate and descends, and the lifter plate drives the centre gripping unit and descends to the culture dish position, and the plant inserts in the culture medium, when the browning phenomenon appears, can mention plant tissue once more at any time.
As shown in fig. 1 to 6, the grabbing unit includes a second motor 17, a second gear 18 and a first disc 19 are fixedly connected to an output shaft of the second motor 17 from inside to outside in sequence, and a semicircular notch 20 is formed in an edge of the first disc 19;
the top surface of the lifting plate 7 is fixedly connected with one end of a mounting bracket 21, the other end of the mounting bracket 21 is fixedly connected with a mounting plate 22, the mounting plate 22 is perpendicular to the ground, a first mounting table 23 is slidably mounted in a third sliding groove 25 on the mounting plate 22 through a third sliding block 24 on the bottom surface, a second motor 17 is mounted on the first mounting table 23, the mounting plate 22 is also fixedly connected with an electric telescopic rod 26 parallel to the third sliding groove 25, and a push rod 27 of the electric telescopic rod 26 is fixedly connected with the first mounting table 23;
the bottom surface of the lifting plate 7 is fixedly connected with hollow first cylinders 28 which are uniformly distributed around the circle center of the second gear 18, the number of the first cylinders 28 is 4, the first cylinders 28 are all positioned above the culture dish 3, the outer wall of the circumference of one end, away from the lifting plate 7, of each first cylinder 28 is fixedly connected with a limit ring table 29, the first cylinders 28 are sleeved with slidable first rings 30, the first cylinders 28 are further sleeved with springs 31, and the springs 31 are positioned between the first rings 30 and the limit ring tables 29;
the limiting ring table 29 is evenly hinged with one end of a plurality of C-shaped clamping blocks 32 around the circle center, the other ends of the C-shaped clamping blocks 32 can be matched with the culture dish 3, the back parts of the C-shaped clamping blocks 32 are hinged with one end of a first hinge rod 33, the other end of the first hinge rod 33 is hinged with the first circular ring 30, the top surface of the first circular ring 30 is fixedly connected with one end of a first ejector rod 34, and the other end of the first ejector rod 34 can vertically slide to penetrate through the lifting plate 7 and can be matched with the first circular disc 19.
In the embodiment, under the motion of the electric telescopic rod, the first mounting table slides in the third sliding groove and has two working positions, when the first mounting table is located at a high position, the first disc is not in contact with all the first ejector rods, the second gear on the second motor only drives the clamping unit to rotate, the spring jacks up the first ring, the first ring drives the first hinge rod, the first hinge rod pulls up the C-shaped clamping block, and the C-shaped clamping block is opened and is not in contact with the culture dish;
when the disc is positioned at a low position, the first disc descends, the first ejector rod is pressed down, the first ejector rod presses down the first circular ring, the first circular ring compresses the spring, the first circular ring drives the first hinge rod, the first hinge rod presses down the C-shaped clamping block, the C-shaped clamping block is closed, and the culture dish is clamped;
when the semicircular gap is aligned with any one first ejector rod, the C-shaped clamping block at the position can not be closed even if the first disc descends, so that only N-1 culture dishes are grabbed each time, when only one browned culture dish is provided, the semicircular gap is aligned with the position, the grabbing unit grabs and ascends the rest culture dishes, the conveyor belt moves to convey the culture dishes away and stops, a worker conveys the next batch of culture dishes into the box and places the culture dishes at the corresponding positions, the conveyor belt conveys the new culture dishes to the lower part of the lifting unit, when a plurality of browned culture dishes are provided, the semicircular gap is aligned with any one browned culture dish, the grabbing unit grabs and ascends the rest culture dishes, the conveyor belt moves to convey the browned culture dishes away and stops, the lifting unit descends to place the grabbed culture dishes back to the conveyor belt, the first disc rotates again, the semicircular gap is aligned with the rest browned culture dishes, the grabbing unit is lifted after grabbing again until only normal culture dishes are left on the grabbing unit, the box body is opened again by a worker, the culture dishes in the next batch are sent into the box body and placed at the corresponding positions of the conveyor belt, and the culture dishes are sent to the lower part of the lifting unit by the conveyor belt;
the grabbing unit can selectively give up the culture dish according to the browning quantity, and always keep the normal culture dish without replacing all the culture dishes at one time, so that the consumption of the culture medium is reduced.
As shown in fig. 6, the clamping unit includes first rotating rods 35 with the same number as the first cylinders 28, the first rotating rods 35 are uniformly distributed around the center of the second gear 18 and rotatably mounted on the lifting plate 7, one end of each first rotating rod 35, which is close to the conveyor belt 4, extends from the hollow first cylinder 28, and a left clamping block 36 and a right clamping block 37 which can be opened and closed are hinged on the outer wall of the circumference, and the other end of each first rotating rod 35 extends out of the top surface of the lifting plate 7 and is fixedly connected with a third gear 38 engaged with the second gear 18.
In the embodiment, the left clamping block and the right clamping block are pulled open, the plant roots and stems are placed in the gap between the left clamping block and the right clamping block, the left clamping block and the right clamping block are slightly folded, the plant roots and stems are clamped, the second gear can drive the third gear to rotate, the third gear drives the first rotating rod and the left clamping block and the right clamping block to rotate, the rotation of the plant roots and stems in a culture medium is realized, the culture medium can be stirred occasionally, the oxygen content of the culture medium is increased, the situation that plant tissues are always fixed and the nutrients are absorbed unevenly can be avoided.
As shown in fig. 1, the storage unit includes a storage chamber 39, the storage chamber 39 is fixedly connected in the box body 1 and is located above the conveyor belt 4, a communicating storage chamber 39 and an electric control nozzle 40 capable of being matched with the culture dish 3 are installed on the bottom end surface of the storage chamber 39, a second ultraviolet lamp 41 is further installed on one side of the electric control nozzle 40, a culture medium feeding port 42 is opened on the top wall of the storage chamber 39, and a third ultraviolet lamp 43 is installed on the inner side top wall of the storage chamber 39.
In this embodiment, the conveyer belt drives the culture dish of new batch that the staff put into and moves to locker room below, and second ultraviolet lamp disinfects the culture dish, and in automatically controlled shower nozzle pours into the culture medium into the culture dish, third ultraviolet lamp still can disinfect to the culture medium in the locker room.
As shown in FIG. 1, a culture dish inlet 44 is formed in the rear side wall of the box body 1, a culture dish collecting box 45 is fixedly connected in the box body 1, the culture dish collecting box 45 is positioned below the front side of the conveyor belt 4, and a culture dish outlet 46 is formed in the side wall of the culture dish collecting box 45.
In this embodiment, the staff sends into the box with the culture dish from the box rear side, and in the culture dish collecting box was sent into to the conveyer belt after the culture dish brown, the staff can open culture dish export clearance useless culture dish and culture medium after the work.
As shown in fig. 1, a third motor 47 is fixedly connected to a side wall of the box 1, and an output shaft of the third motor 47 is fixedly connected to one transmission shaft 5 of the conveyor belt 4.
In this embodiment, the third motor drives the conveyor belt to move.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting the same, and although the present invention is described in detail with reference to the above embodiments, those of ordinary skill in the art should understand that: modifications and equivalents may be made to the embodiments of the invention without departing from the spirit and scope of the invention, which is to be covered by the claims.

Claims (7)

1. A plant tissue culture device with a switching function comprises a box body (1), wherein a control terminal (2) is installed on the outer side wall of the box body (1), and is characterized in that a clamping unit for clamping plant tissues, a culture dish transmission unit for transmitting a culture dish (3), a grabbing unit for grabbing the culture dish (3), a lifting unit for controlling the lifting of the clamping unit and the grabbing unit, and a storage unit for storing a culture medium are arranged in the box body (1);
the culture dish transmission unit comprises a conveyor belt (4), the conveyor belt (4) is movably arranged on two side walls of the box body (1) through two transmission shafts (5) and is positioned at the lowest end of the box body (1), the movement direction of the conveyor belt (4) is the front side, the clamping unit, the grabbing unit and the lifting unit are all positioned above the culture dish transmission unit, the clamping unit and the grabbing unit are arranged at the lifting end of the lifting unit, the clamping unit can lift and extend into a culture dish (3) of the culture dish transmission unit, the storage unit is positioned above the culture dish transmission unit and is positioned at the rear side of the lifting unit, and the outlet end of the storage unit can be communicated with the culture dish transmission unit;
two first grooves (6) capable of placing the culture dish (3) are uniformly formed in the conveyor belt (4) along the length direction.
2. The plant tissue culture apparatus with switching function according to claim 1, the lifting unit comprises a lifting plate (7), the lifting plate (7) is slidably arranged in a first sliding groove (10) and a second sliding groove (11) on two side walls of the box body (1) through a first sliding block (8) and a second sliding block (9) which are fixedly connected on two sides, the first sliding groove (10) and the second sliding groove (11) are perpendicular to the ground, one end of the first sliding groove (10) and one end of the second sliding groove (11) are located on the bottom surface of the box body (1), the other end of the first sliding groove (10) and the other end of the second sliding groove (11) are located on two adjacent side walls of one end of the top wall of the box body (1), a first ultraviolet lamp (12) and a plant feeding port (13) are further respectively arranged on the two adjacent side walls, one end, extending into the first sliding groove (10), of the first sliding block (8) is further fixedly connected with a first;
the first motor (15) is fixedly connected to the side wall of the box body (1), an output shaft of the first motor (15) can rotatably extend into the first sliding chute (10) and is fixedly connected with a first gear (16), and the first gear (16) is meshed with the first rack (14).
3. The plant tissue culture device with the switching function according to claim 2, wherein the grabbing unit comprises a second motor (17), a second gear (18) and a first disc (19) are fixedly connected to an output shaft of the second motor (17) from inside to outside in sequence, and a semicircular notch (20) is formed in the edge of the first disc (19);
the top surface of the lifting plate (7) is fixedly connected with one end of a mounting bracket (21), the other end of the mounting bracket (21) is fixedly connected with a mounting plate (22), the mounting plate (22) is vertical to the ground, a first mounting platform (23) is slidably mounted in a third sliding groove (25) on the mounting plate (22) through a third sliding block (24) on the bottom surface, a second motor (17) is mounted on the first mounting platform (23), an electric telescopic rod (26) parallel to the third sliding groove (25) is further fixedly connected on the mounting plate (22), and a push rod (27) of the electric telescopic rod (26) is fixedly connected with the first mounting platform (23);
the bottom surface of the lifting plate (7) is fixedly connected with hollow first cylinders (28) which are uniformly distributed around the circle center of the second gear (18), the number of the first cylinders (28) is 4, the first cylinders (28) are all positioned above the culture dish (3), the outer wall of the circumference of one end, away from the lifting plate (7), of the first cylinder (28) is fixedly connected with a limit ring table (29), the first cylinder (28) is sleeved with a slidable first ring (30), the first cylinder (28) is further sleeved with a spring (31), and the spring (31) is positioned between the first ring (30) and the limit ring table (29);
the limiting ring platform (29) is uniformly hinged with one end of a plurality of C-shaped clamping blocks (32) around the circle center, the other ends of the C-shaped clamping blocks (32) can be matched with a culture dish (3), the back of each C-shaped clamping block (32) is hinged with one end of a first hinge rod (33), the other ends of the first hinge rods (33) are hinged with a first circular ring (30), the top surface of the first circular ring (30) is fixedly connected with one end of a first ejector rod (34), and the other end of the first ejector rod (34) can slide up and down to penetrate through a lifting plate (7) and can be matched with a first circular disc (19).
4. The plant tissue culture device with the switching function according to claim 3, wherein the clamping units comprise first rotating rods (35) with the same number as the first cylinders (28), the first rotating rods (35) are uniformly distributed around the circle center of the second gear (18) and rotatably mounted on the lifting plate (7), one end of each first rotating rod (35) close to the conveyor belt (4) extends out of the hollow first cylinder (28), a left clamping block (36) and a right clamping block (37) which can be opened and closed are hinged to the outer wall of the circumference of the first rotating rod, and the other end of each first rotating rod (35) extends out of the top surface of the lifting plate (7) and is fixedly connected with a third gear (38) meshed with the second gear (18).
5. The plant tissue culture device with the switching function according to claim 4, wherein the storage unit comprises a storage chamber (39), the storage chamber (39) is fixedly connected in the box body (1) and located above the conveyor belt (4), an electric control nozzle (40) which is communicated with the storage chamber (39) and can be matched with the culture dish (3) is installed at the bottom end face of the storage chamber (39), a second ultraviolet lamp (41) is further installed at one side of the electric control nozzle (40), a culture medium feeding port (42) is formed in the top wall of the storage chamber (39), and a third ultraviolet lamp (43) is installed on the top wall of the inner side of the storage chamber (39).
6. The plant tissue culture device with the switching function according to claim 5, wherein a culture dish inlet (44) is formed on the rear side wall of the box body (1), a culture dish collecting box (45) is fixedly connected in the box body (1), the culture dish collecting box (45) is positioned below the front side of the conveyor belt (4), and a culture dish outlet (46) is formed on the side wall of the culture dish collecting box (45).
7. The plant tissue culture device with switching function according to claim 6, characterized in that a third motor (47) is fixed on the side wall of the box body (1), and the output shaft of the third motor (47) is fixed with a transmission shaft (5) of the conveyor belt (4).
CN202110428113.1A 2021-04-21 2021-04-21 Plant tissue culture device with switching function Withdrawn CN112970586A (en)

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