CN220079030U

CN220079030U - Automatic filling mechanism of cell culture medium

Info

Publication number: CN220079030U
Application number: CN202321565002.6U
Authority: CN
Inventors: 李锐
Original assignee: Chengdu Shengjisaier Biotechnology Co ltd
Current assignee: Chengdu Shengjisaier Biotechnology Co ltd
Priority date: 2023-06-19
Filing date: 2023-06-19
Publication date: 2023-11-24
Anticipated expiration: 2033-06-19

Abstract

The utility model relates to an automatic filling mechanism of a cell culture medium, which comprises a base, wherein a culture dish is placed on the upper surface of the base, a supporting rod is fixed on the upper surface of the base, a fixed box is fixed at the top end of the supporting rod, a filling component is arranged in the fixed box, a material adding component is arranged in the fixed box, the filling component comprises a first electric push rod fixed on the upper surface of the fixed box, and a feeding pipe is fixed between the inner top wall and the inner bottom wall of the fixed box. This automatic filling mechanism of cell culture medium, through the cooperation of first electric putter, can drive the head rod and remove to drive the push pedal and remove, through the removal of push pedal, can drive sealed pad and remove, thereby extrude the inside gas of inlet pipe and culture medium, through the cooperation of spring, can provide a reaction force for the push pedal at the in-process that the push pedal removed, thereby make the push pedal can keep at uniform velocity and steady when removing.

Description

Automatic filling mechanism of cell culture medium

Technical Field

The utility model relates to the technical field of cell culture, in particular to an automatic filling mechanism of a cell culture medium.

Background

The culture medium is a nutrient medium which is used for the growth and reproduction of microorganisms, plants or animals (or tissues) and is prepared from different nutrient substances, and generally contains a plurality of substances such as carbohydrates, nitrogen-containing substances, inorganic salts (including trace elements), vitamins, water and the like, and the culture medium is not only a basic substance for providing cell nutrition and promoting cell proliferation, but also a living environment for cell growth and reproduction.

As disclosed in chinese patent publication No. (CN 212833796U), an automatic cell culture medium filling device is disclosed, which comprises a base, a support, a constant filling device and a fast switching device, wherein the support is arranged on the base, the constant filling device is arranged on the support, the constant filling device comprises a charging barrel, a charging pipe, an electromagnetic valve, a pressure pump, a charging port and a flow sensor, the charging barrel is arranged on the support, the charging pipe is symmetrically arranged at the bottom of the side wall of the charging barrel, the charging pipe is connected with the charging barrel in a penetrating way, the electromagnetic valve is arranged at one end of the charging pipe far away from the charging barrel, the pressure pump is arranged on the charging barrel, the output end of the pressure pump penetrates through the upper wall of the charging barrel and is arranged in the charging barrel, the charging port is arranged on the upper wall of the charging barrel, the charging port is detachably provided with a sealing plug, the flow sensor is arranged at one end of the charging pipe close to the charging barrel, and the flow sensor monitors the flow of the culture medium passing through the charging pipe in real time.

Disclosure of Invention

Aiming at the defects of the prior art, the utility model provides an automatic filling mechanism of a cell culture medium, which has the advantage of keeping the flow speed of the culture medium, and solves the problems that the culture medium is in accelerated motion and is difficult to move in a uniform speed state when being pressurized by a pressure pump, the speed of the culture medium is difficult to control by the pressure pump, and the practicability is influenced.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the automatic filling mechanism of the cell culture medium comprises a base, wherein a culture dish is placed on the upper surface of the base, a supporting rod is fixed on the upper surface of the base, a fixed box is fixed at the top end of the supporting rod, a filling component is arranged in the fixed box, and a material adding component is arranged in the fixed box;

the filling assembly comprises a first electric push rod fixed on the upper surface of the fixed box, a feeding pipe is fixed between the inner top wall and the inner bottom wall of the fixed box, an output shaft of the first electric push rod penetrates through the upper surface of the fixed box and extends to the inner side of the feeding pipe, a first connecting rod is fixed, a push plate is fixed at the bottom end of the first connecting rod, a spring is fixed between the upper surface of the push plate and the inner top wall of the fixed box, and a sealing gasket is fixed on the lower surface of the push plate.

Further, the number of the supporting rods is four, and the supporting rods are uniformly distributed at four corners of the lower surface of the fixed box.

Further, the outer diameter of the push plate is smaller than that of the sealing gasket, and the outer diameter of the sealing gasket is matched with the inner diameter of the feeding pipe.

Further, the lower surface intercommunication of fixed box has the discharging pipe, the other end of discharging pipe runs through and extends to the inside of culture dish, the outside intercommunication of discharging pipe has the bleeder valve.

Further, add material subassembly including fixing the second electric putter in fixed box left side, the output shaft of second electric putter runs through and extends to the inside of fixed box, and is fixed with the second connecting rod, the other end of second connecting rod is fixed with the sprue, the outside of sprue is fixed with the sealing washer.

Further, the feed hole has been seted up to the outside of inlet pipe, the internal diameter in feed hole and the internal diameter looks adaptation of sealing washer.

Further, the outside intercommunication of inlet pipe has the intake pipe, the other end of intake pipe runs through and extends to the right side of fixed box, the outside intercommunication of intake pipe has the admission valve.

Compared with the prior art, the technical scheme of the utility model has the following beneficial effects:

1. this automatic filling mechanism of cell culture medium, through the cooperation of first electric putter, can drive the head rod and remove to drive the push pedal and remove, through the removal of push pedal, can drive sealed pad and remove, thereby extrude the inside gas of inlet pipe and culture medium, through the cooperation of spring, can provide a reaction force for the push pedal at the in-process that the push pedal removed, thereby make the push pedal can keep at uniform velocity and steady when removing.

2. This automatic filling mechanism of cell culture medium, through the cooperation of second electric putter, can drive the second connecting rod and remove to drive the shutoff piece and remove, through the removal of shutoff piece, can drive the sealing washer and remove, thereby shutoff with the feed port, after the inside culture medium of inlet pipe uses up, can open the feed port, make the culture medium in the fixed box flow into in the inlet pipe.

Drawings

FIG. 1 is a schematic diagram of the structure of the present utility model;

FIG. 2 is a schematic illustration of a fill assembly according to the present utility model;

FIG. 3 is a schematic view of the additive assembly of the present utility model.

In the figure: 1 base, 2 culture dish, 3 bracing pieces, 4 fixed boxes, 5 filling components, 501 first electric putter, 502 inlet pipe, 503 first connecting rod, 504 push pedal, 505 springs, 506 sealing gasket, 507 discharging pipe, 508 discharge valve, 6 filling components, 601 second electric putter, 602 second connecting rod, 603 blocking piece, 604 sealing washer.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1, an automatic filling mechanism for cell culture medium in this embodiment includes a base 1, a culture dish 2 is placed on the upper surface of the base 1 for culturing cells or microorganisms, a support rod 3 is fixed on the upper surface of the base 1, a fixing box 4 is fixed on the top end of the support rod 3, the support rod 3 supports the fixing box 4, the inside of the fixing box 4 is filled with culture medium, a filling component 5 is arranged in the fixing box 4, the culture medium is uniformly filled into the culture dish 2, and a material adding component 6 is arranged in the fixing box 4 so as to circularly fill the culture medium into the culture dish 2.

The number of the supporting rods 3 is four, and the supporting rods are uniformly distributed at four corners of the lower surface of the fixed box 4, so that the stability of the fixed box 4 is improved.

Referring to fig. 2, in order to inject culture medium into the culture dish 2 at a constant speed, the filling assembly 5 in this embodiment includes a first electric push rod 501 fixed on the upper surface of the fixed box 4, a feed pipe 502 fixed between the inner top wall and the inner bottom wall of the fixed box 4, an output shaft of the first electric push rod 501 penetrates through the upper surface of the fixed box 4 and extends to the inner side of the feed pipe 502, and a first connecting rod 503 is fixed, the output shaft of the first electric push rod 501 drives the first connecting rod 503 to move, a push plate 504 is fixed at the bottom end of the first connecting rod 503, the first connecting rod 503 drives the push plate 504 to move, a spring 505 is fixed between the upper surface of the push plate 504 and the inner top wall of the fixed box 4, the push plate 504 stretches the spring 505 in the moving process, the stretched spring 505 provides an upward reaction force to the push plate 504, so that the push plate 504 can keep a constant speed stable when moving, a sealing pad 506 is fixed on the lower surface of the push plate 504, and the sealing pad 506 presses air and culture medium inside the feed pipe 502 when moving, so that the culture medium enters the culture dish 2 at a constant speed, and the culture medium is filled inside the culture dish 2.

In this example, the external diameter of push pedal 504 is less than the external diameter of sealing washer 506, and sealing washer 506's external diameter and inlet pipe 502 internal diameter looks adaptation make sealing washer 506's downside space keep sealed, avoid influencing the efficiency of annotating the material, and fixed box 4's lower surface intercommunication has discharging pipe 507, and the other end of discharging pipe 507 runs through and extends to the inside of culture dish 2, and in the culture dish 2 was led into from discharging pipe 507 to the culture medium, the outside intercommunication of discharging pipe 507 had discharge valve 508.

Referring to fig. 3, in order to circulate and inject the culture medium into the culture dish 2, the feeding assembly 6 in the embodiment includes a second electric push rod 601 fixed on the left side of the fixed box 4, an output shaft of the second electric push rod 601 penetrates and extends into the fixed box 4, and a second connecting rod 602 is fixed, the output shaft of the second electric push rod 601 drives the second connecting rod 602 to move, a blocking block 603 is fixed at the other end of the second connecting rod 602, the second connecting rod 602 drives the blocking block 603 to move, a sealing ring 604 is fixed at the outer side of the blocking block 603, and the blocking block 603 drives the sealing ring 604 to move.

In this example, the feed port has been seted up in the outside of inlet pipe 502, the internal diameter of feed port and the internal diameter looks adaptation of sealing washer 604 to make sealing washer 604 carry out the shutoff to the feed port, so as to add the material in the inlet pipe 502, the outside intercommunication of inlet pipe 502 has the intake pipe, the other end of intake pipe runs through and extends to the right side of fixed box 4, the outside intercommunication of intake pipe has the admission valve, when making the inside material of adding of inlet pipe 502, inside pressure is linked together with the external world, thereby guarantee smooth material of adding.

The working principle of the embodiment is as follows:

(1) First electric putter 501 is opened, first electric putter's 501 output shaft drives head rod 503 and removes, head rod 503 drives push pedal 504 and removes, push pedal 504 can stretch to spring 505 in the in-process that removes, spring 505 after receiving the extension provides ascending reaction force to push pedal 504, make push pedal 504 can keep steady at the uniform velocity when removing, push pedal 504 removes and drives sealing pad 506 and remove, sealing pad 506 extrudees the inside air of inlet pipe 502 and culture medium when removing, make the culture medium enter into culture dish 2, thereby fill the culture medium for the inside at the uniform velocity of culture dish 2.

(2) After the culture medium in the feed pipe 502 is filled, the discharge valve 508 is closed, the air inlet valve is opened, the second electric push rod 601 is opened, the output shaft of the second electric push rod 601 drives the second connecting rod 602 to move, the second connecting rod 602 drives the block 603 to move, the block 603 drives the sealing ring 604 to take out the block 603 from the feed hole, the culture medium in the fixed box 4 flows into the feed pipe 502 from the feed hole, at the moment, the first electric push rod 501 is opened again, the output shaft of the first electric push rod 501 moves upwards, the culture medium is pumped into the feed pipe 502, the push plate 504 returns to the initial position, the air inlet valve is closed after the material filling is completed, the discharge valve 508 is opened, and the filling can be continued.

It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. An automatic filling mechanism of cell culture medium, includes base (1), its characterized in that: the culture dish is characterized in that a culture dish (2) is placed on the upper surface of the base (1), a supporting rod (3) is fixed on the upper surface of the base (1), a fixing box (4) is fixed at the top end of the supporting rod (3), a filling component (5) is arranged in the fixing box (4), and a material adding component (6) is arranged in the fixing box (4);

the filling assembly (5) comprises a first electric push rod (501) fixed on the upper surface of the fixed box (4), a feeding pipe (502) is fixed between the inner top wall and the inner bottom wall of the fixed box (4), an output shaft of the first electric push rod (501) penetrates through the upper surface of the fixed box (4) and extends to the inner side of the feeding pipe (502), a first connecting rod (503) is fixed, a push plate (504) is fixed at the bottom end of the first connecting rod (503), a spring (505) is fixed between the upper surface of the push plate (504) and the inner top wall of the fixed box (4), and a sealing gasket (506) is fixed on the lower surface of the push plate (504).

2. An automatic cell culture medium filling mechanism according to claim 1, wherein: the number of the supporting rods (3) is four, and the supporting rods are uniformly distributed at four corners of the lower surface of the fixed box (4).

3. An automatic cell culture medium filling mechanism according to claim 1, wherein: the outer diameter of the pushing plate (504) is smaller than that of the sealing gasket (506), and the outer diameter of the sealing gasket (506) is matched with the inner diameter of the feeding pipe (502).

4. An automatic cell culture medium filling mechanism according to claim 1, wherein: the lower surface of fixed box (4) communicates there is discharging pipe (507), the other end of discharging pipe (507) runs through and extends to the inside of culture dish (2), the outside intercommunication of discharging pipe (507) has bleeder valve (508).

5. An automatic cell culture medium filling mechanism according to claim 1, wherein: the material adding assembly (6) comprises a second electric push rod (601) fixed on the left side of the fixed box (4), an output shaft of the second electric push rod (601) penetrates through and extends to the inside of the fixed box (4), a second connecting rod (602) is fixed, a blocking block (603) is fixed at the other end of the second connecting rod (602), and a sealing ring (604) is fixed on the outer side of the blocking block (603).

6. An automated cell culture medium feeding mechanism according to claim 5, wherein: and a feeding hole is formed in the outer side of the feeding pipe (502), and the inner diameter of the feeding hole is matched with the inner diameter of the sealing ring (604).

7. An automated cell culture medium feeding mechanism according to claim 5, wherein: the outside intercommunication of inlet pipe (502) has the intake pipe, the other end of intake pipe runs through and extends to the right side of fixed box (4), the outside intercommunication of intake pipe has the admission valve.