CN220183290U - Automatic fluid infusion device for immune cell in-vitro culture - Google Patents
Automatic fluid infusion device for immune cell in-vitro culture Download PDFInfo
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- CN220183290U CN220183290U CN202321538696.4U CN202321538696U CN220183290U CN 220183290 U CN220183290 U CN 220183290U CN 202321538696 U CN202321538696 U CN 202321538696U CN 220183290 U CN220183290 U CN 220183290U
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- 239000012530 fluid Substances 0.000 title claims abstract description 74
- 238000001802 infusion Methods 0.000 title claims abstract description 47
- 238000000338 in vitro Methods 0.000 title claims abstract description 27
- 210000002865 immune cell Anatomy 0.000 title claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 156
- 238000007789 sealing Methods 0.000 claims abstract description 41
- 230000001502 supplementing effect Effects 0.000 claims abstract description 39
- 238000007667 floating Methods 0.000 claims abstract description 15
- 230000002093 peripheral effect Effects 0.000 claims description 6
- 210000004027 cell Anatomy 0.000 abstract description 10
- 238000002637 fluid replacement therapy Methods 0.000 abstract description 4
- 238000012544 monitoring process Methods 0.000 abstract description 3
- 238000009423 ventilation Methods 0.000 description 7
- 210000004698 lymphocyte Anatomy 0.000 description 6
- 230000005484 gravity Effects 0.000 description 5
- 210000001744 T-lymphocyte Anatomy 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000028993 immune response Effects 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 210000003719 b-lymphocyte Anatomy 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000009469 supplementation Effects 0.000 description 2
- 210000000612 antigen-presenting cell Anatomy 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003714 granulocyte Anatomy 0.000 description 1
- 210000003630 histaminocyte Anatomy 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000000865 mononuclear phagocyte system Anatomy 0.000 description 1
- 210000001539 phagocyte Anatomy 0.000 description 1
- 210000004180 plasmocyte Anatomy 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to an automatic fluid infusion device for in-vitro culture of immune cells, which comprises a liquid storage component and a culture component. The liquid storage assembly comprises a fixing frame and a liquid storage bottle, and a supporting rod is arranged at the bottom of the fixing frame; the bottom end of the liquid storage bottle is provided with a first sealing cover, and a liquid discharge pipe communicated with the liquid storage bottle is arranged on the first sealing cover; the culture assembly comprises a culture bottle arranged below the liquid storage bottle, a second sealing cover is arranged on the culture bottle, a vent pipe is detachably connected to the second sealing cover in a threaded manner, and the bottom end of the vent pipe is hinged to the rotating rod; one end of the rotating rod is provided with a floating ball, and the other end of the rotating rod is sequentially connected with a connecting rope, a connecting rod and a sliding block; the sliding block is in sliding connection with the inner wall of the liquid inlet pipe arranged on the liquid storage bottle. According to the utility model, the liquid level of the culture solution is monitored through the cooperation between the floating ball and the sliding block, manual monitoring is not needed, automatic fluid replacement is realized, and the problems of untimely fluid replacement and the like are effectively avoided; and the liquid supplementing is carried out through the connecting pipe, so that the pollution probability of cells is reduced.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to an automatic fluid infusion device for in-vitro culture of immune cells.
Background
Immune cells are commonly known as leukocytes, and include lymphocytes, various phagocytes, etc., and also specifically refer to lymphocytes that recognize antigens, produce specific immune responses, etc. Lymphocytes are the basic components of the immune system and are widely distributed in the body, and mainly T lymphocytes and B lymphocytes are activated by antigen stimulation, divide and proliferate and generate specific immune responses. In addition to T lymphocytes and B lymphocytes, there are K lymphocytes and NK lymphocytes, four types. T lymphocytes are a multi-functional cell population. In addition to lymphocytes, cells involved in the immune response are plasma cells, granulocytes, mast cells, antigen presenting cells, and cells of the mononuclear phagocyte system.
During cell culture, the cells consume the culture fluid and make-up is required. If the fluid infusion is not timely, serious consequences can be caused; the staff needs to manually supplement the liquid for many times, so that the efficiency is low, and resources are wasted; if the syringe is used for multiple fluid infusion, the syringe is of an open structure, so that cells are easy to be polluted in the fluid infusion process.
At present, no effective solution is proposed for solving the problems of untimely liquid supplementation, low manual liquid supplementation efficiency, high probability of cell pollution and the like in the related technology.
Disclosure of Invention
The utility model aims at overcoming the defects in the prior art, and provides an automatic liquid supplementing device for in-vitro culture of immune cells, so as to solve the problems of untimely liquid supplementing, low manual liquid supplementing efficiency, high probability of cell pollution and the like in the related technologies.
In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows:
an automatic fluid infusion device for immune cell in-vitro culture comprises a liquid storage component and a culture component which is positioned below the liquid storage component and communicated with the liquid storage component, wherein:
the liquid storage assembly comprises a fixing frame and a liquid storage bottle detachably arranged on the fixing frame, and support rods are respectively arranged at four corners of the fixing frame; the bottom opening of the liquid storage bottle is detachably provided with a first sealing cover, the first sealing cover is provided with a liquid discharge pipe communicated with the liquid storage bottle, and the output end of the liquid discharge pipe is communicated with the culture assembly through a connecting pipe;
the culture assembly comprises a culture bottle arranged below the liquid storage bottle, a second sealing cover is detachably arranged at the bottom of the culture bottle, a vent pipe is detachably connected to the second sealing cover through threads, and a rotating rod is hinged to the bottom end of the vent pipe; one end of the rotating rod is provided with a floating ball which is connected with a sliding block through a connecting rope and a connecting rod, and the sliding block is arranged in a sliding cylinder at the top end of the liquid inlet pipe in a sliding way; and the side wall of the top end of the liquid inlet pipe is provided with a liquid supplementing port, and the input end of the liquid supplementing port is communicated with the connecting pipe.
Further, the liquid inlet pipe is L-shaped, the lower end of the liquid inlet pipe is communicated with the liquid discharge pipe through the connecting pipe, a liquid supplementing pipe is arranged at a liquid supplementing port of the liquid inlet pipe, and the top end of the liquid inlet pipe is communicated with the liquid supplementing pipe.
Further, the inner peripheral wall of the top end of the liquid inlet pipe is provided with two limit protrusions which are arranged up and down corresponding to the liquid supplementing port, and the sliding block is slidably arranged between the two limit protrusions.
Further, the liquid supplementing pipe is L-shaped, the output end of the liquid supplementing pipe is downward arranged, and the inner diameter of the liquid supplementing pipe is smaller than that of the liquid inlet pipe.
Further, a sealing protrusion is arranged on the vent pipe in a surrounding mode, and the sealing protrusion is located at the bottom of the second sealing cover.
Further, the breather pipe is vertically arranged, a plurality of vent holes are formed in the peripheral wall of the lower end at intervals, the top end of the breather pipe is communicated with the output end of the breather pipe, and an air filter is arranged at the input end of the breather pipe.
Further, the output end of the liquid discharge pipe is provided with a control valve.
Further, the bottom ends of the four supporting rods are connected with a base which is horizontally arranged, and the culture flask is arranged at the top of the base.
Further, the supporting rod is of a telescopic structure and is detachably connected with the clamping hole through the clamping block so as to adjust the length of the supporting rod.
Further, a liquid supplementing port is formed in the side wall of the upper middle portion of the liquid storage bottle, and a third sealing cover is detachably arranged on the liquid supplementing port.
Compared with the prior art, the utility model has the following technical effects:
(1) According to the automatic liquid supplementing device for the immune cell in-vitro culture, the liquid level of the culture liquid is monitored through the cooperation between the floating ball and the sliding block, manual monitoring is not needed, automatic liquid supplementing is achieved, the problems that liquid supplementing is not timely, manual liquid supplementing efficiency is low and the like are effectively avoided, liquid supplementing is carried out through the connecting pipe, and the pollution probability of cells is reduced;
(2) According to the automatic liquid supplementing device for the in-vitro culture of the immune cells, ventilation of the inner cavity of the culture bottle is realized through the detachable connection between the ventilation pipe and the second sealing cover, and the rotation rod is flexibly rotated through the hinge connection between the ventilation pipe and the rotation rod, so that the device is simple in structure, low in cost and convenient to maintain;
(3) According to the automatic liquid supplementing device for the in-vitro culture of the immune cells, the length of the supporting rod is adjusted to match the liquid level requirements of culture bottles with different heights or different culture liquids, so that the adaptation degree of the liquid storage assembly is improved.
Drawings
FIG. 1 is a schematic diagram (I) of an automatic fluid infusion device for in vitro culture of immune cells according to the present utility model;
FIG. 2 is a schematic diagram of the three-dimensional structure of an automatic fluid infusion device for in vitro culture of immune cells according to the utility model;
FIG. 3 is a schematic view of the partial cross-section structure of an automatic fluid infusion device for in vitro culture of immune cells according to the utility model;
FIG. 4 is a schematic diagram showing the partial structure of a liquid storage component in an automatic liquid replenishing device for in vitro culture of immune cells;
FIG. 5 is a schematic view of a partial sectional structure of a culture assembly in a fluid-filled state in an automatic fluid-filled device for in vitro culture of immune cells according to the present utility model;
FIG. 6 is an enlarged schematic view at A in FIG. 5;
FIG. 7 is a schematic view of a partial cross-sectional structure of a culture assembly in a non-fluid-replacement state in an automatic fluid-replacement device for in vitro culture of immune cells according to the present utility model;
FIG. 8 is a schematic view showing the partial cross-sectional structure of a culture assembly in an automatic fluid infusion device for in vitro culture of immune cells according to the present utility model.
Wherein the reference numerals are as follows:
100. a liquid storage component; 101. a fixing frame; 102. a liquid storage bottle; 103. a support rod; 104. a first sealing cover; 105. a liquid discharge pipe; 106. a connecting pipe; 107. a control valve; 108. a base; 109. a fluid supplementing port; 110. a third sealing cover;
200. a culturing assembly; 201. a culture bottle; 202. a second sealing cover; 203. a vent pipe; 204. a rotating lever; 205. a floating ball; 206. a connecting rope; 207. a connecting rod; 208. a slide block; 209. a liquid inlet pipe; 210. a fluid supplementing pipe; 211. a sealing protrusion; 212. a vent hole; 213. a ventilation pipe; 214. an air filter.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
It should be noted that, without conflict, the embodiments of the present utility model and features of the embodiments may be combined with each other.
The utility model is further described below with reference to the drawings and specific examples, which are not intended to be limiting.
An automatic fluid infusion device for in vitro culture of immune cells, as shown in fig. 1-3, comprises a fluid storage assembly 100 and a culture assembly 200 which is positioned below and communicated with the fluid storage assembly 100.
The liquid storage assembly 100 comprises a fixing frame 101 and a liquid storage bottle 102 detachably arranged on the fixing frame 101, and support rods 103 are respectively arranged at four corners of the fixing frame 101; the bottom opening of the liquid storage bottle 102 is detachably provided with a first sealing cover 104, the first sealing cover 104 is provided with a liquid discharge pipe 105 communicated with the liquid storage bottle 102, and the output end of the liquid discharge pipe 105 is communicated with the culture assembly 200 through a connecting pipe 106; the culture assembly 200 comprises a culture bottle 201 arranged below the liquid storage bottle 102, a second sealing cover 202 is detachably arranged at the bottom of the culture bottle 201, a vent pipe 203 is detachably connected to the second sealing cover 202 in a threaded manner, and a rotating rod 204 is hinged to the bottom end of the vent pipe 203; one end of the rotating rod 204 is provided with a floating ball 205 which is connected with a sliding block 208 through a connecting rope 206 and a connecting rod 207, and the sliding block 208 is arranged in a sliding cylinder at the top end of a liquid inlet pipe 209 in a sliding way; and the side wall of the top end of the liquid inlet pipe 209 is provided with a liquid supplementing port, and the input end of the liquid supplementing port is communicated with the connecting pipe 106.
Specifically, the liquid storage bottle 102 stores the supplementary liquid, the liquid level of the supplementary liquid is not higher than the top of the sliding cylinder at the top end of the liquid inlet pipe 209, the bottom end of the vent pipe 203 is of a sealing structure, and the top end of the vent pipe is of an opening structure; the connecting rod 207 is vertically arranged at the top of the sliding block 208, and the top of the connecting rod is connected with the end head of the rotating rod 204 through the connecting rope 206.
When the liquid level of the culture solution in the culture flask 201 drops, the floating ball 205 moves downward along with the liquid level of the culture solution, and the buoyancy of the floating ball 205 is greater than the self gravity of the sliding block 208, so that the rotating rod 204 rotates; the connecting rope 206 is pulled tightly under the rotation of the rotating rod 204, so that the pulling slide block 208 slides upwards, the liquid supplementing port on the liquid inlet pipe 209 is opened, and then the supplementing liquid in the liquid storage bottle 102 is injected into the inner cavity of the culture bottle 201 through the liquid inlet pipe 209; after the liquid level of the culture solution in the culture flask 201 rises to the designated liquid level, i.e. after the fluid infusion is completed, the floating ball 205 moves upwards along with the liquid level of the culture solution, the connecting rope 206 is gradually loosened, the sliding block 208 moves downwards under the action of self gravity, the density of the sliding block 208 is larger, so that the self gravity of the sliding block 208 is larger, the self gravity of the sliding block 208 can overcome the pressure of the outlet end of the connecting pipe 106, and the output end of the liquid inlet pipe 209 is in a closed state, i.e. fluid infusion is stopped. The liquid level of the culture solution is monitored through the floating ball 205, manual monitoring is not needed, automatic fluid infusion is achieved, the problems that fluid infusion is not timely, manual fluid infusion is low in efficiency and the like are effectively avoided, fluid infusion is carried out through the connecting pipe 106, and the pollution probability of cells is reduced.
In some embodiments, scale marks are arranged on the outer wall of the liquid storage bottle 102, so that the liquid volume in the inner cavity of the liquid storage bottle is convenient to observe.
In some embodiments, the input end of the liquid drain tube 105 is disposed through the first sealing cover 104 and is disposed in the inner cavity of the liquid storage bottle 102.
In some of these embodiments, the flask 201 is transparent to facilitate viewing of its interior.
In some embodiments, the floating ball 205 needs to be cleaned and replaced periodically, and the floating ball 205 is detachably and screwed with the rotating rod 204, so that cleaning and maintenance are facilitated.
In some of these embodiments, the connecting cord 206 is detachably connected to the connecting rod 207, and the vent tube 203 is detachably screwed to the second seal cap 202, so that the rotating rod 204 is cleaned periodically.
Further, as shown in fig. 3 and 5 to 7, the liquid inlet pipe 209 is L-shaped, the lower end thereof is communicated with the liquid discharge pipe 105 through the connecting pipe 106, the liquid supplementing pipe 210 is arranged at the liquid supplementing port of the liquid inlet pipe 209, and the top end of the liquid inlet pipe 209 is communicated with the liquid supplementing pipe 210.
Specifically, the opening and closing of the fluid infusion tube 210 is controlled by the slider 208 through the fluid infusion port of the fluid infusion tube 209.
In some of these embodiments, the fluid refill port of the fluid refill tube 210 is located in the lower middle portion of the flask 201.
Further, as shown in fig. 5 to 7, two limiting protrusions are disposed at the inner peripheral wall of the top end of the liquid inlet tube 209 and corresponding to the liquid supplementing opening, and the sliding block 208 is slidably disposed between the two limiting protrusions.
In some embodiments, the distance between the two limit protrusions is greater than or equal to twice the height of the slider 208.
Specifically, when the top of the slider 208 contacts the limiting protrusion, the input end of the fluid infusion tube 210 is in an open state; when the bottom of the sliding block 208 is contacted with the limiting protrusion, the input end of the fluid infusion tube 210 is in a sealing state.
Further, as shown in fig. 6, the fluid infusion tube 210 is in an L-shape, and the output end thereof is disposed downward, and the inner diameter thereof is smaller than the inner diameter of the fluid inlet tube 209, so that the slider 208 can sufficiently seal the input end of the fluid infusion tube 210.
Further, as shown in fig. 1, in order to improve the tightness of the connection between the vent pipe 203 and the second sealing cover 202, a sealing protrusion 211 is disposed on the vent pipe 203 in a ring, and the sealing protrusion 211 is located at the bottom of the second sealing cover 202.
Further, as shown in fig. 8, the vent pipe 203 is vertically disposed, wherein a plurality of vent holes 212 are disposed on the peripheral wall of the lower end at intervals, the top end of the vent pipe is communicated with the output end of the vent pipe 213, and the input end of the vent pipe 213 is provided with an air filter 214.
In some embodiments, the vent pipe 203 and the vent pipe 213 are detachably connected through a clamping block and a clamping hole.
In some of these embodiments, vent tube 203 is removably threaded with vent tube 213.
In some of these embodiments, the vent holes 212 include, but are not limited to, vent round holes, vent square holes, and the like.
In some embodiments, 4 vent holes 212 are provided, spaced apart from each other, on the same horizontal plane.
In some of these embodiments, the air filter 214 is a miniature water blocking air filter.
Further, as shown in fig. 1 to 4, in order to effectively control the start and stop of the fluid infusion, the output end of the fluid discharge tube 105 is provided with a control valve 107 to control the opening and closing of the fluid discharge tube 105.
In some of these embodiments, the control valve 107 is a control switch.
Further, as shown in fig. 1 to 3, the bottom ends of the four support rods 103 are connected to the horizontally arranged base 108, and the culture flask 201 is placed on top of the base 108.
In some embodiments, the culture flask 201 is placed on top of the base 108, which facilitates overall handling and reduces handling difficulty.
Further, as shown in fig. 1 to 3, in order to match with the culture flasks 201 of different heights, the supporting rods 103 are of a telescopic structure, and the supporting rods 103 are detachably connected with the clamping holes through clamping blocks to adjust the length of the supporting rods.
In some embodiments, the adaptation of the fluid storage assembly 100 is improved by adjusting the length of the support bar 103 to match the fluid level requirements of different height flasks 201 or different fluids.
Further, as shown in fig. 2 and 4, a fluid-filling port 109 is provided on a side wall of the upper middle portion of the fluid-storage bottle 102, and a third sealing cover 110 is detachably provided on the fluid-filling port 109.
In some embodiments, when the replenishing liquid in the liquid storage bottle 102 is insufficient, the liquid discharge pipe 105 is closed by adjusting the control valve 107, namely, the replenishing liquid is stopped; the third sealing cover 110 is disassembled, and liquid is replenished to the inner cavity of the liquid storage bottle 102 through the liquid replenishing port 109; after the fluid infusion is completed, the third sealing cover 109 is detachably connected with the fluid infusion port 110, and the liquid discharge pipe 105 is opened by adjusting the control valve 107, so that the liquid storage assembly 100 can automatically infuse the fluid.
The working principle of the utility model is as follows:
when the liquid level of the culture solution in the culture flask 201 drops, the floating ball 205 moves downward along with the liquid level of the culture solution to rotate the rotating rod 204;
the rotating rod 204 tightens the connecting rope 206 in the rotating process to drive the sliding block 208 to slide upwards, so that the top of the sliding block 208 is contacted with the limiting bulge, namely the output end of the liquid inlet pipe 209 is communicated with the liquid supplementing pipe 210;
the replenishing liquid in the liquid storage bottle 102 is injected into the inner cavity of the culture bottle 201 through the liquid discharge pipe 105, the connecting pipe 106, the liquid inlet pipe 209 and the replenishing liquid pipe 210 in sequence;
after the liquid level of the culture solution in the culture bottle 201 rises to the designated liquid level, i.e. after the fluid is replenished, the floating ball 205 moves upwards along with the liquid level of the culture solution, so that the rotating rod 204 rotates;
during the rotation of the rotating rod 204, the connecting rope 206 is in a loose state, and the sliding block 208 moves downwards under the action of self gravity, so that the bottom of the sliding block 208 is in contact with the limiting protrusion, that is, the output end of the liquid inlet pipe 209 is not communicated with the liquid supplementing pipe 210, that is, liquid supplementing is stopped.
When the cavity of the flask 201 is ventilated, the gas outside the flask 201 is discharged to the cavity of the flask 201 through the air filter 214, the ventilation tube 213, the ventilation tube 203, and the ventilation hole 212 in this order.
In some embodiments, when the replenishing liquid in the liquid storage bottle 102 is insufficient, the liquid discharge pipe 105 is closed by adjusting the control valve 107, namely, the replenishing liquid is stopped; the third sealing cover 110 is disassembled, and liquid is replenished to the inner cavity of the liquid storage bottle 102 through the liquid replenishing port 109; after the fluid infusion is completed, the third sealing cover 109 is detachably connected with the fluid infusion port 110, and the liquid discharge pipe 105 is opened by adjusting the control valve 107, so that the liquid storage assembly 100 can automatically infuse the fluid.
The foregoing description is only illustrative of the preferred embodiments of the present utility model and is not to be construed as limiting the scope of the utility model, and it will be appreciated by those skilled in the art that equivalent substitutions and obvious variations may be made using the description and illustrations of the present utility model, and are intended to be included within the scope of the present utility model.
Claims (10)
1. An automatic fluid infusion device for in vitro culture of immune cells, which is characterized by comprising a fluid storage assembly (100) and a culture assembly (200) which is positioned below the fluid storage assembly (100) and communicated with the fluid storage assembly, wherein:
the liquid storage assembly (100) comprises a fixing frame (101) and a liquid storage bottle (102) detachably arranged on the fixing frame (101), and supporting rods (103) are respectively arranged at four corners of the fixing frame (101); a first sealing cover (104) is detachably arranged at the bottom opening of the liquid storage bottle (102), a liquid discharge pipe (105) communicated with the liquid storage bottle (102) is arranged on the first sealing cover (104), and the output end of the liquid discharge pipe (105) is communicated with the culture assembly (200) through a connecting pipe (106);
the culture assembly (200) comprises a culture bottle (201) arranged below the liquid storage bottle (102), a second sealing cover (202) is detachably arranged at the bottom of the culture bottle (201), a vent pipe (203) is detachably connected to the second sealing cover (202) in a threaded manner, and a rotating rod (204) is hinged to the bottom end of the vent pipe (203); one end of the rotating rod (204) is provided with a floating ball (205) which is connected with a sliding block (208) through a connecting rope (206) and a connecting rod (207), and the sliding block (208) is arranged in a sliding cylinder at the top end of the liquid inlet pipe (209) in a sliding way; and the side wall of the top end of the liquid inlet pipe (209) is provided with a liquid supplementing port, and the input end of the liquid supplementing port is communicated with the connecting pipe (106).
2. The automatic fluid infusion device for in-vitro culture of immune cells according to claim 1, wherein the fluid inlet pipe (209) is L-shaped, the lower end of the fluid inlet pipe is communicated with the fluid outlet pipe (105) through the connecting pipe (106), a fluid infusion pipe (210) is arranged at a fluid infusion port of the fluid inlet pipe (209), and the top end of the fluid inlet pipe (209) is communicated with the fluid infusion pipe (210).
3. The automatic liquid supplementing device for in-vitro culture of immune cells according to claim 2, wherein two limit protrusions which are arranged up and down are arranged on the inner peripheral wall of the top end of the liquid inlet pipe (209) corresponding to the liquid supplementing port, and the sliding block (208) is slidably arranged between the two limit protrusions.
4. The automatic fluid infusion device for the in vitro culture of immunocytes according to claim 2, characterized in that said fluid infusion tube (210) is provided in an L-shape, with its output end facing downwards and with an internal diameter smaller than the internal diameter of said fluid intake tube (209).
5. The automatic fluid infusion device for in vitro culture of immune cells according to claim 1, wherein a sealing protrusion (211) is arranged on the vent pipe (203) in a surrounding manner, and the sealing protrusion (211) is positioned at the bottom of the second sealing cover (202).
6. The automatic fluid infusion device for the in-vitro culture of immune cells according to claim 1, wherein the vent pipe (203) is vertically arranged, a plurality of vent holes (212) are formed in the peripheral wall of the lower end at intervals, the top end of the vent holes is communicated with the output end of the vent pipe (213), and an air filter (214) is arranged at the input end of the vent pipe (213).
7. The automatic fluid infusion device for the in vitro culture of immune cells according to claim 1, wherein the output end of the fluid discharge tube (105) is provided with a control valve (107).
8. The automatic fluid infusion device for in vitro culture of immune cells according to claim 1, wherein the bottom ends of the four support rods (103) are connected with a horizontally arranged base (108), and the culture flask (201) is arranged at the top of the base (108).
9. The automatic fluid infusion device for in vitro culture of immunocytes according to claim 1, wherein the supporting rod (103) is of a telescopic structure, and the supporting rod (103) is detachably connected with the clamping hole through a clamping block so as to adjust the length of the supporting rod.
10. The automatic fluid infusion device for the in-vitro culture of immune cells according to claim 1, wherein a fluid infusion port (109) is arranged on the side wall of the middle upper part of the fluid infusion bottle (102), and a third sealing cover (110) is detachably arranged on the fluid infusion port (109).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202321538696.4U CN220183290U (en) | 2023-06-16 | 2023-06-16 | Automatic fluid infusion device for immune cell in-vitro culture |
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CN202321538696.4U CN220183290U (en) | 2023-06-16 | 2023-06-16 | Automatic fluid infusion device for immune cell in-vitro culture |
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CN220183290U true CN220183290U (en) | 2023-12-15 |
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CN202321538696.4U Active CN220183290U (en) | 2023-06-16 | 2023-06-16 | Automatic fluid infusion device for immune cell in-vitro culture |
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