CN220224164U - Closed lentiviral vector culture apparatus - Google Patents
Closed lentiviral vector culture apparatus Download PDFInfo
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- CN220224164U CN220224164U CN202320492930.8U CN202320492930U CN220224164U CN 220224164 U CN220224164 U CN 220224164U CN 202320492930 U CN202320492930 U CN 202320492930U CN 220224164 U CN220224164 U CN 220224164U
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- 238000009434 installation Methods 0.000 claims abstract description 11
- 239000011261 inert gas Substances 0.000 claims abstract description 10
- 239000007789 gas Substances 0.000 claims description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 238000007789 sealing Methods 0.000 claims description 17
- 238000005192 partition Methods 0.000 claims description 14
- 239000007921 spray Substances 0.000 claims description 8
- 230000000694 effects Effects 0.000 abstract description 13
- 238000000034 method Methods 0.000 abstract description 11
- 238000001125 extrusion Methods 0.000 abstract description 8
- 230000001771 impaired effect Effects 0.000 abstract description 2
- 238000011081 inoculation Methods 0.000 abstract description 2
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- 230000008878 coupling Effects 0.000 description 6
- 238000010168 coupling process Methods 0.000 description 6
- 238000005859 coupling reaction Methods 0.000 description 6
- 241000700605 Viruses Species 0.000 description 5
- 238000010586 diagram Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 241000902900 cellular organisms Species 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of culture devices, in particular to a closed lentiviral vector culture device, which comprises: the top of the connecting shell is hinged with a cover body; the placing structure is arranged in the connecting shell; through setting up and placing the structure, can dismantle the installation easy and simple to handle through rotatory apron, can place the culture dish that the inoculation is good after dismantling the apron and cultivate in the standing groove, rotatory apron screw up again can, at this in-process can be through receiving clamping ring, the connecting rod, spring and extrusion ring with inert gas injection buffer air bag's inside, thereby can fill the space between culture dish and the standing groove under buffer air bag's effect, buffer air bag can increase the frictional force with between the culture dish, can avoid culture dish and the impaired condition of standing groove inner wall collision when the device receives the collision, buffer air bag can reduce the impact of collision to the influence of culture dish simultaneously, the protection effect of culture in-process has been improved.
Description
Technical Field
The utility model relates to the technical field of culture devices, in particular to a closed lentiviral vector culture device.
Background
The virus is a non-cellular organism which is tiny in individual, has a simple structure, contains only one nucleic acid, and has to be parasitized in living cells and proliferated in a replication mode, and has important significance for research of molecular biology and cell biology.
Through retrieving, chinese patent discloses a closed lentiviral vector culture apparatus (bulletin number CN 212451412U), and this patent technique a plurality of sealing members seal and can be better to the culture save and cultivate, can effectually prevent to infect and spill, reduced the loss, but the protection effect to the culture dish in the cultivation process is relatively poor, and the device is received and is led to the culture dish to collide with its inner wall easily and cause the damage when bumping, consequently, the person skilled in the art provides a closed lentiviral vector culture apparatus to solve the problem that proposes in the above-mentioned background art.
Therefore, a closed lentiviral vector culture apparatus was proposed to solve the above-mentioned problems.
Disclosure of Invention
The utility model aims to solve the problems and provide a closed lentiviral vector culture device which solves the problem that the prior device has poor protective effect on a culture dish.
The utility model realizes the aim through the following technical scheme, and the closed slow virus vector culture device comprises: the top of the connecting shell is hinged with a cover body; the placing structure is arranged in the connecting shell and is used for placing the culture dish; the auxiliary structure is arranged on the surface of the connecting shell and is used for adjusting the culture environment in the connecting shell.
The utility model provides a gas storage device, including connecting shell inner wall, including the fixed connection, the standing board of connecting shell inner wall is put to the structure, evenly distributed's standing groove has been seted up at the top of standing board, the top fixedly connected with evenly distributed of standing board and the go-between that sets up with the coaxial heart of standing groove, the outer fringe of go-between is provided with the external screw thread, the top of standing board is provided with the apron corresponding with the standing groove, the recess that matches with the go-between has been seted up to the bottom of apron, the internal screw thread that matches with the external screw thread has been seted up to the inner wall of recess, the top fixedly connected with knob of apron, the gas storage chamber of a plurality of evenly distributed has been seted up to the inside of standing board, the gas storage chamber with adjacent the coaxial heart of standing groove sets up, the inside packing of gas storage chamber has inert gas, the inner wall sliding connection of gas storage chamber has the stripper plate, the top fixedly connected with evenly distributed's of stripper plate, the upper end of connecting rod runs through out the standing board and fixedly connected with sealing ring, the surface cover of connecting rod is equipped with spring, the inner wall embedding of standing groove installs the buffer gasbag, the surface intercommunication of buffer gasbag has the pipe, adjacent gas storage chamber.
Preferably, the auxiliary structure comprises a mounting shell fixedly connected to one side of the connecting shell, through holes which are uniformly distributed and communicated with the inside of the connecting shell are formed in one side of the mounting shell, air inlets which are uniformly distributed are formed in the other side of the mounting shell, one-way valves are arranged on the inner walls of the air inlets, a partition plate is fixedly connected to the inner walls of the mounting shell, and return guide holes are formed in the surfaces of the partition plate.
Preferably, water is filled between the bottom of the partition plate and the installation shell, a water pump is arranged on the inner bottom wall of the installation shell, a connecting pipe is communicated with the top of the water pump, the other end of the connecting pipe penetrates through the partition plate and is communicated with a shunt pipe, and spray heads which are evenly distributed are communicated with the surface of the shunt pipe.
Preferably, the auxiliary structure further comprises a collecting box fixedly connected to the other side of the connecting shell, an air pump is arranged on the inner wall of the collecting box, a communicating pipe is communicated with the top of the air pump, evenly distributed delivery pipes are communicated with the surface of the communicating pipe, and the other end of the delivery pipe is communicated with the inside of the connecting shell.
The beneficial effects of the utility model are as follows:
1. through setting up and placing the structure, can dismantle easy and simple to handle through rotatory apron, can place the culture dish that inoculates after dismantling the apron and cultivate in the standing groove, rotatory apron screw up again can, can be through receiving clamping ring, the connecting rod, inside that spring and extrusion ring were filled inert gas into buffer gasbag in this process, thereby can fill the space between culture dish and the standing groove under buffer gasbag's effect, buffer gasbag can increase the frictional force with between the culture dish, can avoid the condition that culture dish and the standing groove inner wall collide impaired when the device receives the collision, buffer gasbag can reduce the impact of collision to the culture dish simultaneously, the protection effect in the culture process has been improved;
2. through setting up auxiliary structure, can replace the inside gas of coupling shell fast, simultaneously under the effect of water pump, connecting pipe, shunt tubes and shower nozzle, can be according to actual conditions the gas of the inside of the substitution admission coupling shell humidification to reach the effect of carrying out the humidification to coupling shell inside, easy and simple to handle can adjust coupling shell internal environment fast.
Drawings
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is a cross-sectional perspective view of the present utility model;
FIG. 3 is a schematic view of a placement structure according to the present utility model;
FIG. 4 is an enlarged view of A in FIG. 3;
FIG. 5 is a schematic illustration of the connection of a bulkhead to a mounting case according to the present utility model;
fig. 6 is a schematic diagram showing connection between the air pump and the communicating tube according to the present utility model.
In the figure: 1. a connection housing; 101. a cover body; 2. placing a structure; 201. placing a plate; 202. a placement groove; 203. a connecting ring; 204. a knob; 205. a cover plate; 206. a gas storage chamber; 207. a connecting rod; 208. a seal ring; 209. a spring; 210. a buffer air bag; 211. a conduit; 212. a groove; 3. an auxiliary structure; 301. a mounting shell; 302. a through hole; 303. an air inlet hole; 304. a partition plate; 305. a guide-back hole; 306. a one-way valve; 307. a water pump; 308. a connecting pipe; 309. a shunt; 310. a spray head; 311. a collection box; 312. an air pump; 313. a communicating pipe; 314. and a delivery tube.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
The specific implementation method comprises the following steps: as shown in FIGS. 1 to 6, a closed lentiviral vector culture apparatus comprising: the top of the connecting shell 1 is hinged with a cover body 101; the placing structure 2 is arranged in the connecting shell 1, and the placing structure 2 is used for placing the culture dish; auxiliary structure 3, auxiliary structure 3 sets up in the surface of coupling shell 1, and auxiliary structure 3 is used for the regulation to the cultivation environment in the coupling shell 1.
As shown in fig. 1-6, the placement structure 2 comprises a placement plate 201 fixedly connected to the inner wall of the connection shell 1, uniformly distributed placement grooves 202 are formed in the top of the placement plate 201, uniformly distributed connection rings 203 which are coaxially arranged with the placement grooves 202 are fixedly connected to the top of the placement plate 201, external threads are arranged on the outer edges of the connection rings 203, a cover plate 205 corresponding to the placement grooves 202 is arranged on the top of the placement plate 201, grooves 212 matched with the connection rings 203 are formed in the bottom of the cover plate 205, internal threads matched with the external threads are formed in the inner walls of the grooves 212, knobs 204 are fixedly connected to the top of the cover plate 205, a plurality of uniformly distributed gas storage cavities 206 are formed in the interior of the placement plate 201, the gas storage cavities 206 are coaxially arranged with the adjacent placement grooves 202, inert gas is filled in the interior of the gas storage cavities 206, extrusion plates are slidingly connected to the inner walls of the gas storage cavities 206, uniformly distributed connection rods 207 are fixedly connected to the top of the extrusion plates, the upper ends of the connection rods 207 penetrate through the placement plate 201 and are fixedly connected with sealing rings 208, springs 209 are sleeved on the surfaces of the connection rods 207 (the lower ends of the springs 209 are fixedly connected to the tops of the extrusion rings 203, the upper ends of the springs 206 are fixedly connected with the top walls of the extrusion rings 202, the upper ends of the connection rings 206 are fixedly connected with the inner walls of the gas storage cavities 210, the gas storage cavities 210 are fixedly connected with the gas storage cavities 211, and the gas storage cavities 211 are fixedly connected with the gas storage cavities 211 are respectively, and the gas storage cavities are connected with the gas storage cavities 211 and the gas storage cavities are respectively;
placing a culture dish inoculated with a virus vector in the placing groove 202, rotating and screwing the cover plate 205 through the knob 204 and the connecting ring 203, wherein the cover plate 205 can extrude the sealing ring 208 to move downwards in the process, so that the sealing ring 208 can drive the connecting rod 207 to gradually insert into the air storage cavity 206, meanwhile, the extruding ring extrudes inert gas in the air storage cavity 206, the extruded inert gas is injected into the buffer air bag 210 through the guide pipe 211, so that the buffer air bag 210 can bulge to fill a gap between the culture dish and the placing groove 202, friction force between the buffer air bag 210 and the culture dish can be increased under the action of the buffer air bag 210 of a rubber material component, thereby achieving the effect of limiting the position of the culture dish, ensuring that the placement of the culture dish is more stable, avoiding the situation that the culture dish collides with the inner wall of the placing plate 201, simultaneously, the buffer air bag 210 can play the buffering effect, reducing the influence of impact force generated by collision on the culture dish, simultaneously, extruding the sealing ring 208 through the bottom of the sealing ring 205 after the cover plate 205 is rotationally screwed, the extruded sealing ring 208 can enable the connecting rod 207 to top the bottom of the connecting rod 207 to fill a gap between the culture dish and the placing groove 202 under the action of the spring 209, thereby further improving the sealing effect of the sealing ring 212, and further avoiding the situation that the virus vector is inserted into the gap between the sealing ring 201 and the placing groove and the sealing plate 201;
when the culture dish is required to be taken out, the cover plate 205 and the connecting ring 203 can be separated by reversely rotating the cover plate 205, and the extrusion ring and the connecting rod 207 can be reset under the action of the spring 209 in the process, so that the space between the bottom of the extrusion ring and the gas storage cavity 206 is increased, at the moment, the inside inert gas is introduced into the gas storage cavity 206 for storage through the guide pipe 211 under the action of the buffer air bag 210, and meanwhile, the buffer air bag 210 contracts to release the limit of the culture dish, so that a certain gap exists between the culture dish and the inner wall of the placing groove 202 for convenient taking.
As shown in fig. 1-6, the auxiliary structure 3 comprises a mounting shell 301 fixedly connected to one side of the connection shell 1, a through hole 302 which is uniformly distributed and communicated with the inside of the connection shell 1 is formed in one side of the mounting shell 301, an air inlet 303 which is uniformly distributed is formed in the other side of the mounting shell 301, a one-way valve 306 is arranged on the inner wall of the air inlet 303, a partition plate 304 is fixedly connected to the inner wall of the mounting shell 301, a guide hole 305 is formed in the surface of the partition plate 304, water is filled between the bottom of the partition plate 304 and the mounting shell 301, a water pump 307 is arranged on the inner bottom wall of the mounting shell 301, a connecting pipe 308 is communicated with the top of the water pump 307, the other end of the connecting pipe 308 penetrates through the partition plate 304 and is communicated with a shunt tube 309, a spray nozzle 310 which is uniformly distributed is communicated with the surface of the shunt tube 309 (the spray nozzle 310 is preferably a spray nozzle, the contact area between spray water and air flow can be increased, and the specific model number is selected according to actual conditions), the auxiliary structure 3 further comprises a collecting box 311 fixedly connected to the other side of the connection shell 1, an air pump 312 is arranged on the inner wall of the collecting box 312, a top of the air pump is communicated with a guide pipe 313, a distributed, a guide pipe 314 is communicated with the other end of the guide pipe 314 is communicated with the uniform, and the guide pipe 314 is communicated with the inside of the connection shell 1;
when the gas needs to be replaced in the connecting shell 1, the air pump 312 can be started, the gas in the connecting shell 1 can be pumped into the collecting box 311 through the communicating pipe 313 and the delivery pipe 314 for storage so as to be convenient for subsequent unified treatment, as the air in the connecting shell 1 is pumped out, the inside of the connecting shell is negative pressure, the outside air can pass through the one-way valve 306 to enter the inside of the mounting shell 301, and the outside air is led into the inside of the connecting shell 1 through the through hole 302, so that the effect of replacing the gas is achieved;
when the inside of the connection shell 1 needs to be humidified, the water below the partition plate 304 can be guided into the inside of the connecting pipe 308 by starting the water pump 307 under the action of the water pump 307, and sprayed on the partition plate 304 under the action of the shunt pipe 309 and the spray head 310, at the moment, the air pump 312 is started to pump the air inside the connection shell 1, and the external air can be contacted with the sprayed water when passing through the installation shell 301, so that the moist air flow is formed to enter the inside of the connection shell 1, and the effect of humidifying the inside of the connection shell 1 is achieved.
When the culture dish inoculation device is used, the cover body 101 is opened, the cover plate 205 is separated from the connecting ring 203 through the knob 204, the placing groove 202 is exposed, the inoculated culture dish is placed in the placing groove 202, the cover plate 205 and the connecting ring 203 are screwed through the knob 204, the cover plate 205 can be extruded to move downwards in the process, the connecting rod 207 and the extruding ring are driven to move downwards synchronously through the sealing ring 208, inert gas in the gas storage cavity 206 can be extruded, the extruded inert gas is injected into the buffer air bag 210 through the guide tube 211, the buffer air bag 210 can bulge to fill a gap between the culture dish and the placing groove 202, friction force between the buffer air bag 210 and the culture dish can be increased under the action of the buffer air bag 210 of a rubber material component, so that the effect of limiting the position of the culture dish is achieved, the placing is more stable, the condition that the culture dish collides with the inner wall of the placing plate 201 and is damaged due to shaking in the culture process is avoided, meanwhile, the bottom of the cover plate 205 can be matched with the sealing ring 208 to strengthen the sealing performance between the sealing ring, the two conditions that the inner side of the placing groove 202 is prevented from affecting the culture of virus carrier, meanwhile, the gap between the connecting ring 203 and the inner part of the placing groove 212 can be inserted into the buffer air bag 210, the gap between the inner part of the connecting ring 203 can be cut off, and the placing plate 201 can be further sealed;
when the inside of the connection shell 1 needs to be replaced by gas, the outside air can be led into the inside of the connection shell 1 by starting the air pump 312, meanwhile, the original gas inside the connection shell 1 is led into the collection box 311 for storage, water can be sprayed on the partition plate 304 by starting the water pump 307 when humidification is needed, the air inside the connection shell 1 is pumped by starting the air pump 312 again, the outside air can be contacted with the sprayed water when passing through the installation shell 301, so that moist air flow is formed into the inside of the connection shell 1, and the effect of humidifying the inside of the connection shell 1 is achieved.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (4)
1. A closed lentiviral vector culture apparatus comprising:
the connecting shell (1), the top of the connecting shell (1) is hinged with a cover body (101);
the placing structure (2) is arranged in the connecting shell (1), and the placing structure (2) is used for placing the culture dish;
the auxiliary structure (3) is arranged on the surface of the connecting shell (1), and the auxiliary structure (3) is used for adjusting the environment in the connecting shell (1);
the placing structure (2) comprises a placing plate (201) fixedly connected to the inner wall of the connecting shell (1), placing grooves (202) which are uniformly distributed are formed in the top of the placing plate (201), connecting rings (203) which are uniformly distributed and are coaxial with the placing grooves (202) are fixedly connected to the top of the placing plate (201), and external threads are arranged on the outer edges of the connecting rings (203);
the top of the placing plate (201) is provided with a cover plate (205) corresponding to the placing groove (202), the bottom of the cover plate (205) is provided with a groove (212) matched with the connecting ring (203), the inner wall of the groove (212) is provided with an internal thread matched with the external thread, and the top of the cover plate (205) is fixedly connected with a knob (204);
the inside of placing board (201) has offered a plurality of evenly distributed's gas storage chamber (206), gas storage chamber (206) with adjacent standing groove (202) coaxial setting, the inside of gas storage chamber (206) is filled with inert gas, the inner wall sliding connection of gas storage chamber (206) has the stripper plate, the top fixedly connected with evenly distributed's connecting rod (207) of stripper plate, the upper end of connecting rod (207) runs through out placing board (201) and fixedly connected with sealing ring (208), the surface cover of connecting rod (207) is equipped with spring (209);
the inner wall of the placing groove (202) is embedded with a buffer air bag (210), the surface of the buffer air bag (210) is communicated with a guide pipe (211), and the other end of the guide pipe (211) is communicated with the adjacent air storage cavity (206).
2. The closed lentiviral vector culture apparatus of claim 1, wherein: auxiliary structure (3) are including fixed connection in installation shell (301) of connection shell (1) one side, evenly distributed and with connect shell (1) inside through-hole (302) that are linked together have been seted up to one side of installation shell (301), inlet port (303) that all with the distribution have been seted up to the opposite side of installation shell (301), the inner wall of inlet port (303) is provided with check valve (306), the inner wall fixedly connected with baffle (304) of installation shell (301), guide back hole (305) have been seted up on the surface of baffle (304).
3. The closed lentiviral vector culture apparatus of claim 2, wherein: water is filled between the bottom of the partition plate (304) and the installation shell (301), a water pump (307) is arranged on the inner bottom wall of the installation shell (301), a connecting pipe (308) is communicated with the top of the water pump (307), the other end of the connecting pipe (308) penetrates through the partition plate (304) and is communicated with a shunt pipe (309), and evenly distributed spray heads (310) are communicated with the surface of the shunt pipe (309).
4. A closed lentiviral vector culture apparatus according to claim 3, wherein: the auxiliary structure (3) further comprises a collecting box (311) fixedly connected to the other side of the connecting shell (1), an air pump (312) is arranged on the inner wall of the collecting box (311), a communicating pipe (313) is communicated with the top of the air pump (312), evenly distributed delivery pipes (314) are communicated with the surface of the communicating pipe (313), and the other ends of the delivery pipes (314) are communicated with the inside of the connecting shell (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320492930.8U CN220224164U (en) | 2023-03-15 | 2023-03-15 | Closed lentiviral vector culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320492930.8U CN220224164U (en) | 2023-03-15 | 2023-03-15 | Closed lentiviral vector culture apparatus |
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| Publication Number | Publication Date |
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| CN220224164U true CN220224164U (en) | 2023-12-22 |
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| CN202320492930.8U Active CN220224164U (en) | 2023-03-15 | 2023-03-15 | Closed lentiviral vector culture apparatus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115247122A (en) * | 2022-07-08 | 2022-10-28 | 中南大学湘雅医院 | Nosocomial bacteria sampling culture auxiliary device |
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2023
- 2023-03-15 CN CN202320492930.8U patent/CN220224164U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115247122A (en) * | 2022-07-08 | 2022-10-28 | 中南大学湘雅医院 | Nosocomial bacteria sampling culture auxiliary device |
| CN115247122B (en) * | 2022-07-08 | 2024-04-26 | 中南大学湘雅医院 | Hospital-infection bacterial sampling and culturing auxiliary device |
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