CN109964736A - A kind of preparation process and its application of edible mushroom suspension fluid strain - Google Patents
A kind of preparation process and its application of edible mushroom suspension fluid strain Download PDFInfo
- Publication number
- CN109964736A CN109964736A CN201910359170.1A CN201910359170A CN109964736A CN 109964736 A CN109964736 A CN 109964736A CN 201910359170 A CN201910359170 A CN 201910359170A CN 109964736 A CN109964736 A CN 109964736A
- Authority
- CN
- China
- Prior art keywords
- maintained
- solid
- container
- strain
- suspension
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The present invention provides the preparation processes and its application of a kind of edible mushroom suspension fluid strain, including solid pedigree seed culture medium is fitted into sack or plastic bottle, after solid Primary spawn primary surface is flattened, using steam high-voltage sterilizing, after cooling, culturing room is put into after access test tube stock, the temperature of culturing room is maintained at: 23 ~ 25 DEG C, air humidity is maintained at: 60 ~ 65%, gas concentration lwevel is maintained at: 3000 ~ 4000ppm, it is protected from light culture, until obtaining solid original seed strain；A container is taken, and water, suspending agent are added in container, wherein the ratio of water and suspending agent is 100:1 ~ 2, after being passed through steam heating, stops heating, is cooled to 23 ~ 30 DEG C to container to get sterile suspension；Solid original seed strain is moved on in container, is mixed with the sterile suspension in container and also discloses suspension fluid strain to get suspension fluid strain in the application of edible mushroom preparation field；Suspension obtained by the method for the invention has many advantages, such as that solid spawn is energetic, growing way is prosperous, shelf-stable, resistance are strong, is not easy to degenerate.
Description
Technical field
The present invention relates to the preparation process of field of agricultural products processing more particularly to a kind of edible mushroom suspension fluid strain and its answer
With.
Background technique
Currently, edible fungus species production is based on traditional solid spawn and liquid spawn, both technologies are comparatively
Maturation, it is suitable for mass production；In addition to this, there are also solid liquefaction strain and bacteria suspension strain both strain technologies, but
It is that both strain technologies are also in development test stage, rare large-scale application at present.
Solid spawn: it is general to use " parent species+original seed+cultivar " three-level production of hybrid seeds technique, it is transferred to 1 test tube stock
4-6 bottles of original seeds bottles, every bottle of original seed expands again to be connected in 40-60 bag cultivating kind, and last cultivar mycelia delivers a child again after covering with and produces Bao Pei
Raise mushroom.To accelerate strain bacterium germination speed, need to reserve about 2 ㎝'s of diameter with perforation rod among original seed, cultivar compost
Inoculation hole, so that strain is in charge level and intermediate bacterium germination simultaneously.This method advantage is that spawn activity is good, growing way is prosperous, resistance is strong, suitable
Answer environment capacity strong；The disadvantage is that: strain production of hybrid seeds amount is big, strain individual difference is also big, and the production cycle is long (at least 70 days or more), plants
Charge level and bottom cell age cell age difference 30 days or more are cultivated, mycelia vigor is inconsistent, causes fruiting regularity poor.
Liquid spawn: general to use " parent species+shaking flask culture+first class seed pot culture+second order fermentation tank culture " level Four system
Kind technique, i.e. 1 parent species connect 250ml culture solution level-one shaking flask culture, then obtain 100L liquid bacteria through seeding tank, fermentation tank culture
Kind, 100L liquid spawn can connect 3000 bags of cultivation package or more.This method advantage: (1) production of hybrid seeds is fast, every grade of liquid bacteria of conventional variety
As long as kind prepares 57 days general, completion in level Four technique most 40 days；(2) cell age is consistent, and liquid strain needs to be passed through filtrated air
Air agitation, each position nutrient environment condition relative equilibrium in tank are carried out, mycelia grows also more consistent；(3) shorten cultivation
Period, liquid strain can not only cover charge level, moreover it is possible to penetrate into compost, can with cycle time, the recruitment of cultivation, energy consumption,
The costs such as place are all greatly reduced.(4) breeding cost is low, and not only sowing quantity is few, and liquid spawn is just able to satisfy 5000 by 1 parent species
Bag cultivating packet with kind of a demand, and it is high-efficient to connect bacterium, saves labor.Liquid spawn also has unsatisfactory place: to hardware
More demanding, capital investment is more, is not suitable for small-scale application, strain resistance is weaker, encounters power-off and causes high temperature, anoxic etc.
Poor environment strain is easy to degenerate, or even scraps.In addition, liquid spawn technology is in needle mushroom, mushroom, oyster mushroom, black fungus, Mao Mu
Using more in the principal items such as ear, Pleurotus eryngii, the rare large-scale application of other kinds, there are certain limitations.
(3) solid liquefaction strain is to prepare solid pure culture according to a conventional method first, then smashs solid spawn to pieces, adds
Sterile water is ground into starchiness in pulverizer, and certain multiple sterile water is added, and is then poured into production packet compost with utensil.
Although the liquefaction strain vigor is good, permeability is strong, and germination point is more, since the technology liquefaction step is cumbersome, inoculation method
Fairly simple extensive, solid spawn liquefaction process must add water to be crushed to 80-200 mesh thickness through pulverizer, to strain damage compared with
Greatly, mycelia restores slower, while to be likely to cause thallus mononucleated and lead to not fruiting for vigorous physical stirring, and there are higher
Pollution risk.
Bacteria suspension germination point made from the technology is more, cell age is consistent, and with short production cycle, pollution risk is small, and production cost is low.
But the technology intermediate link is complicated for operation, is required to aseptically complete, there is very big dirt in large-scale production and application
Contaminate risk；In addition the strain that sets out is liquid spawn, only intermediate plus filtering, elution plus the broken link of pigment, substantially and
It is not different using liquid discharge bacterium germination kind as cultivar, on the rare varities practice of the Liquid Cultures slow growth such as Sparassis crispa
There are limitations, and these intermediate links increase unnecessary workload on foot, greatly increase strain infection risk.
Summary of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of preparation works of edible mushroom suspension fluid strain
Skill and its application, technical solution provided by the invention are as follows:
A kind of preparation process of edible mushroom suspension fluid strain, includes the following steps:
Step (1): the preparation of solid original seed strain: solid pedigree seed culture medium is fitted into sack or plastic bottle, by sack or plastics
After solid Primary spawn primary surface in bottle flattens, using steam high-voltage sterilizing, after being cooled to 23 ~ 26 DEG C, access test tube is female
It is put into culturing room after kind, the temperature of culturing room is maintained at: 23 ~ 25 DEG C, air humidity is maintained at: 60 ~ 65%, gas concentration lwevel
It is maintained at: 3000 ~ 4000ppm and is protected from light culture, until obtaining solid original seed strain；
Step (2): sterile suspension preparation: taking a container, and toward addition water, suspending agent in container, wherein water and suspending agent
Ratio is 100:1 ~ 2, after being passed through steam heating 100 ~ 140 minutes, stops heating, is cooled to 23 ~ 30 DEG C to container to get sterile
Suspension；
Step (3): the preparation of suspension fluid strain: the solid original seed strain that step (1) obtains is moved on in container, in container
Sterile suspension mixing is to get suspension fluid strain, and wherein solid original seed strain and sterile suspension volume ratio are 1:20.
Further, the preparation method of solid pedigree seed culture medium described in step (1) includes the following steps:
(1) pretreatment of raw material: sawdust, wheat bran are spare with 50 mesh sieving machine fine screens；
(2) ingredient: the wheat bran 20% after sawdust 78%, fine screen, sucrose 1%, gypsum 1% after taking fine screen obtain solid after mixing
Raw material solid material and water is mixed evenly to get solid pedigree seed culture medium, wherein the water content of solid pedigree seed culture medium
60 62%.
Further, steam high-voltage sterilizing described in step (1) refers to the steam sterilizing 2 when temperature is 121 DEG C
2.5h。
Further, the suspending agent is any in low molecule suspending agent, polymeric retention aid suspension, silicic acid class suspending agent
It is a kind of.
Further, the low molecule suspending agent includes glycerol, syrup；The polymeric retention aid suspension includes Arabic gum
Slurry, agar, methylcellulose, polyvinyl alcohol.
Further, the silicic acid class suspending agent includes alumina silicate, diatomite.
Further, any one of container described in step (2) in triangular flask, shaking flask, fermentor.
The invention also discloses the suspension fluid strain edible mushroom preparation field application.
Further, the edible mushroom preparation method the following steps are included:
S1: aseptically, aseptic gas is accessed in container described in step (3), the container inner pressure is maintained at
Between 0.02 ~ 0.05Mpa, and then the suspension fluid strain in container is accessed into cultivation package；
S2: moving into culture room for inoculated cultivation package, and culture room temperature is maintained at 23 ~ 25 DEG C, and air humidity is maintained at 65 ~
70%, gas concentration lwevel is maintained at 3000 ~ 4000ppm, is protected from light culture 35 ~ 40 days until mycelia walks, continuation After-mature cultivation
35-40 days；
S3: the cultivation package after mycelia is walked moves on to mushroom room, opens cultivation package, and the temperature of mushroom room is maintained at 20 ~ 22 DEG C, sky
Air humidity degree is maintained at 90 ~ 95%, and gas concentration lwevel is maintained at 3000 ~ 4000ppm；
S4: bleaching to charge level mycelia, reduces temperature to 18 ~ 20 DEG C, carries out 4 hours light stimulations, stronger ventilation amount daily；
S5: after mycelia grows up to mushroom flower bud, temperature is adjusted to 16 ~ 18 DEG C, and air humidity is maintained at 60 ~ 70%, daily illumination 4 hours,
Reduce ventilation quantity；
S6: it after mushroom flower bud grows up, can harvest.
Further, the edible mushroom preparation method the following steps are included:
S1: aseptically, aseptic gas is accessed in container described in step (3), the container inner pressure is maintained at
Between 0.02 ~ 0.05Mpa, and then the suspension fluid strain in container is accessed into cultivation package；
S2: moving into culture room for inoculated cultivation package, and culture room temperature is maintained at 23 ~ 25 DEG C, and air humidity is maintained at 65 ~
70%, gas concentration lwevel is maintained at 3000 ~ 4000ppm, is protected from light culture 35 ~ 40 days until mycelia walks, continuation After-mature cultivation
35-40 days；
S3: the cultivation package after mycelia is walked moves on to mushroom room, restores 2-3 days at a temperature of 22-24 DEG C, air humidity is maintained at
85-90%, gas concentration lwevel are maintained at 1500 ~ 2000ppm, carry out illumination in 4 hours daily, and add water moisturizing on ground；
S4: 2-3 days after removal cultivation package lantern ring, when mushroom flower bud is close to sack, should carry out thin flower bud in time, when mushroom handle length up to 10 ~
12cm, mushroom lid diameter reach 4cm, and edge thinning can harvest when opening a business open and flat.
The method of the invention overcomes existing solid spawn, liquid spawn, solid kind of liquefaction strain and bacteria suspension strain
The shortcomings that, the advantages of combining conventional solid strain, liquid spawn, obtained suspension not only has solid spawn vigor
By force, growing way is prosperous, shelf-stable, resistance are strong, be not easy to degenerate and small investment, is suitble to the advantages of different size large-scale production, and
Also have the advantages that the liquid spawn production of hybrid seeds period is short, cell age is consistent, sowing quantity saves, inoculation efficiency is high, production cost is low.
It is embodied in the following aspects:
1, purity high activity is strong, and using test tube stock as the strain that sets out, strain purity and activity are higher, and original seed is with fine screen sawdust
For primary raw material, link is crushed without the strain in bacteria suspension strain and solid liquefaction both new technologies of strain, reduces bacterium
Kind excessive mechanical damage, avoids because of the mononucleated risk of thallus brought by vigorous physical stirring；
2, it is neat to walk the fast fruiting of bacterium, suspension improves liquid suspension degree, reduce strain sedimentation, and pass through gas by the way that suspending agent is added
Dynamic or mechanical stirring, keeps strains'distribution more uniform, and bacterial concentration is low, permeates, three-dimensional bacterium germination walks bacterium faster；
3, small investment is at low cost, on the basis of original seed is got ready, directly prepares suspension use, ready-to-use, does not need to cultivate,
2 days equipment volume of the circular flow are only needed, and traditional liquid strain needs to prepare 5-7 days volume of the circular flow, needs to be equipped with numerous fermentors
With airhandling equipment and greater area of production of hybrid seeds workshop.
4, the period is short quick, and suspension fluid strain is using " test tube stock-original seed suspension " the second level production of hybrid seeds work innovated
Skill saves three-level cultivar link, simplifies production of hybrid seeds technique.It is the conventional variety production of hybrid seeds period parent species 15 days, original seed 30 days, total only to need
45 days, with traditional liquid strain " parent species+shaking flask culture+first class seed pot culture+second order fermentation tank culture " level Four production of hybrid seeds technique
It is close.Suspension liquid preparing process reduces cultivar link compared with original solid spawn, greatly reduces production of hybrid seeds amount and system
In the kind period, technique is more simplified, and the production cycle shortens, and the bacterium germination time shortens 40%, reduces using area, facility, raw material and labor
The consumption of power, reduces costs；
5, efficiency high risk is small, and suspension fluid strain need to only get out original seed and suspension, ready-to-use, avoids Liquid Culture mistake
The risk that power-off, unit exception occur in journey；Inoculation method be under toilet's laminar flow hood environmental condition, it is compacted with big flow industry
Dynamic pump is directly sprayed into suspension in cultivation package by sterile hose.The process flow is not only safe and efficient, easy to operate, and
It can realize that computer automatically controls by peristaltic pump, positioning and quantitative inoculation, strains'distribution is uniform, good penetrability.
Specific embodiment
In order to make those skilled in the art better understand the technical solutions in the application, below in conjunction with the application reality
The attached drawing in example is applied, the technical scheme in the embodiment of the application is clearly and completely described, it is clear that described implementation
Example is merely a part but not all of the embodiments of the present application.Based on the embodiment in the application, this field is common
The application protection all should belong in technical staff's every other embodiment obtained without making creative work
Range.
Embodiment 1
The preparation process of Pleurotus eryngii suspension fluid strain, includes the following steps:
(1) pretreatment of raw material: sawdust, wheat bran are spare with 50 mesh sieving machine fine screens, remove various impurity, it is ensured that particle uniform one
It causes；
(2) ingredient: the wheat bran 20% after sawdust 78%, fine screen, sucrose 1%, gypsum 1% after taking fine screen obtain solid after mixing
Raw material solid material and water is mixed evenly to get solid pedigree seed culture medium, wherein the water content of solid pedigree seed culture medium
60 62%.
(3) prepared by solid original seed strain: prepared solid pedigree seed culture medium is packed into 1100ml plastics seed bottle, charging
Volume about 1000ml, weight of loading 600g, capacity about 0.6g/ml, solid pedigree seed culture medium, surface slightly flatten, intermediate
Do not beat preformed hole；High pressure steam sterilization is carried out according to a conventional method, is sterilized 2 2.5 hours at 121 DEG C, after sterilizing, is placed
The cleaning shop of 100000 grades or more cleanliness is cooled to 23 ~ 26 DEG C or less；Aseptically take the Pleurotus eryngii of one piece of 2cm*2cm
Parent species are connected to solid Primary spawn primary surface, and the test tube stock of 1 20mm*200mm connects 46 bottles of original seeds；After having connect bacterium, it is placed on
Culturing room, the temperature of culturing room keep air humidity in 60% 65% gas concentration lwevel 3000- at 23 25 DEG C
4000ppm is protected from light culture；After mycelium germination material feeding, every 3-5 days Cha Yici bacterium, pollution and the weak strain of growing way, warp are rejected
35 days 30 days, mycelia walked full compost and obtains Pleurotus eryngii solid original seed.
(4) prepared by sterile suspension: 400L tap water being added in 500L fermentor, and adds 1 ~ 2% agar, is passed through
Steam is heated, and the exhaust valve on fermentor is closed behind water boiling 2-3 minutes in tank, continues to heat, keep at 120 DEG C
120 minutes, to get sterile suspensions after temperature in tank is cooled to 25 DEG C.
(5) prepared by suspension fluid strain: according to the ratio of solid original seed weight and suspension volume 1:20, selecting 20 bottles of steps
(3) the Pleurotus eryngii solid original seed obtained aseptically breaks into fine granularity or powdered with electric mixer；Then it opens
Ozonizer is opened to carry out space disinfection 45 minutes, then cleaned around fermentor inoculation mouth with thimerosal, around original seed bottleneck with
And outer surface, sterile wet cotton gloves are put on, according to sterile working, under pyrosphere protection, open fermentor inoculation mouth, access is
Solid original seed through pulverization process, fastens inoculation mouth, is passed through aseptic gas and is uniformly mixed and mixes bacteria suspension bacterium to get Pleurotus eryngii
Kind.
(6) production of cultivation package:
1. by sawdust 15%, bagasse 15%, corncob 20%, cotton seed hulls 15%, wheat bran 24%, dregs of beans 5%, corn flour 3%, lime 2%,
The formula rate of calcium carbonate 1% weighs various raw material for standby；
2. sawdust, corncob are shifted to an earlier date 24 hours addition lime and water is prewetted, cotton seed hulls is individually added water and stirred to drenched, then by its
Stirring is added by raw material in he together, keeps the skin wet, and water content is made to reach 62% ~ 64%, and Pleurotus eryngii culture medium is made after mixing；
3. by Pleurotus eryngii culture medium dress packet after under 0.15Mp steam pressure high pressure sterilization 2 ~ 2.5 hours, after sterilizing, place
The cleaning shop of 100000 grades of cleanliness be cooled to 23 ~ 26 DEG C it is spare.
(7) inoculation and fruiting:
S1: aseptically, being passed through aseptic gas through tertiary filter by air pump, and fermentor internal pressure power is maintained at 0.02
Pleurotus eryngii in container is aseptically mixed bacteria suspension strain by sterile pipeline, with inoculation using pressure by ~ 0.05Mpa
Rifle or peristaltic pump access cultivation package waiting, and every packet meets 30 ~ 35ml of bacteria suspension, containing about solid original seed 1.2g；
S2: moving into culture room for inoculated cultivation package, and culture room temperature is maintained at 23 ~ 25 DEG C, and air humidity is maintained at 65 ~
70%, gas concentration lwevel is maintained at 3000 ~ 4000ppm, is protected from light culture 35 ~ 40 days after mycelia walks, continues After-mature cultivation
35-40 days；
S3: the cultivation package after mycelia is walked moves on to mushroom room, restores 2-3 days at a temperature of 22-24 DEG C, the bag of cultivation package is straightened
Mouthful, lantern ring height is improved, air humidity is maintained at 85-90%, and gas concentration lwevel is maintained at 1500 ~ 2000ppm, carries out 4 daily
Hour illumination (intensity 800Lx) stimulates, and adds water moisturizing on ground, can also according to circumstances carry out space humidification (according to not dredging
Flower bud technique then should be noted that whether the differentiation of mushroom flower bud is smooth, otherwise should magnify sack, increases oxygen concentration, promote the differentiation of mushroom flower bud)；Removal
2-3 days after lantern ring, when mushroom flower bud is close to sack, it should carry out dredging flower bud in time；When mushroom handle length is up to 10 ~ 12cm, mushroom lid diameter reaches 4cm,
Edge thinning can harvest when opening a business open and flat.
Embodiment 2
The preparation process of true pleurotus cornucopiae suspension fluid strain, includes the following steps:
(1) pretreatment of raw material: sawdust, wheat bran are spare with 50 mesh sieving machine fine screens, remove various impurity, it is ensured that particle uniform one
It causes；
(2) ingredient: the wheat bran 20% after sawdust 78%, fine screen, sucrose 1%, gypsum 1% after taking fine screen obtain solid after mixing
Raw material solid material and water is mixed evenly to get solid pedigree seed culture medium, wherein the water content of solid pedigree seed culture medium
60 62%.
(3) prepared by solid original seed strain: prepared solid pedigree seed culture medium is packed into 1100ml plastics seed bottle, charging
Volume about 1000ml, weight of loading 600g, capacity about 0.6g/ml, solid Primary spawn primary surface slightly flatten, and centre is not
Beat preformed hole；High pressure steam sterilization is carried out according to a conventional method, and sterilize 2 2.5h at 121 DEG C, after sterilizing, places 100,000
Grade or more the cleaning shop of cleanliness be cooled to 23 ~ 26 DEG C；The true pleurotus cornucopiae parent species of one piece of 2cm*2cm are aseptically taken to be connected to
Solid Primary spawn primary surface, the test tube stock of 1 20mm*200mm connect 46 bottles of original seeds；After having connect bacterium, it is placed on culturing room, is trained
The temperature of room is supported at 23 25 DEG C, keeps air humidity in 60% 65% gas concentration lwevel 3000-4000ppm, is protected from light training
It supports；After mycelium germination material feeding, every 3-5 days Cha Yici bacterium, pollution and the weak strain of growing way are rejected, through 35 days 30 days, bacterium
Silk walks full compost and obtains true pleurotus cornucopiae solid original seed.
(4) prepared by sterile suspension: 400L tap water being added in 500L fermentor, and adds 1 ~ 2% diatomite, leads to
Enter steam to be heated, the exhaust valve on fermentor is closed behind water boiling 2-3 minutes in tank, continues to heat, be protected at 120 DEG C
It holds 120 minutes, to get sterile suspensions after temperature in tank is cooled to 25 DEG C.
(5) prepared by suspension fluid strain: according to the ratio of solid original seed weight and suspension volume 1:20, selecting 20 bottles of steps
(3) the true pleurotus cornucopiae solid original seed obtained aseptically breaks into fine granularity or powdered with electric mixer；Then it opens
Ozonizer is opened to carry out space disinfection 45 minutes, then cleaned around fermentor inoculation mouth with thimerosal, around original seed bottleneck with
And outer surface, sterile wet cotton gloves are put on, according to sterile working, under pyrosphere protection, open fermentor inoculation mouth, access is
Solid original seed through pulverization process, fastens inoculation mouth, is passed through aseptic gas and is uniformly mixed to get the mixed bacteria suspension bacterium of true pleurotus cornucopiae
Kind.
(6) production of cultivation package:
1. taking 100 parts of the sub- shell of raw material, 10 parts of wheat bran, 5 parts of soybean powder (or cotton benevolence powder), 2 parts of quick lime, the ratio of raw material and water is
1:1.5 adjusts the proportion that PH is 6.5~7.5, cotton seed hulls is individually added water and stirred to drenched, then other raw materials are added together
Stirring, keeps the skin wet, water content is made to reach 63%~65%, true pleurotus cornucopiae culture medium is made after mixing；
2. by true pleurotus cornucopiae culture medium dress packet after under 0.15Mp steam pressure high pressure sterilization 2~2.5 hours, after sterilizing, put
Set 100,000 grades of cleanliness cleaning shop be cooled to 23~26 DEG C it is spare.
(7) inoculation and fruiting:
S1: aseptically, being passed through aseptic gas through tertiary filter by air pump, and fermentor internal pressure power is maintained at 0.02
True pleurotus cornucopiae in container is aseptically mixed bacteria suspension strain by sterile pipeline, with inoculation using pressure by ~ 0.05Mpa
Rifle or peristaltic pump access cultivation package waiting, and every packet meets bacteria suspension 30-35ml, containing about solid original seed 1.2g；
S2: moving into culture room for inoculated cultivation package, and culture room temperature is maintained at 24 ~ 26 DEG C, and air humidity is maintained at 65 ~
70%, gas concentration lwevel is maintained at 3000 ~ 4000ppm, is protected from light culture 35 ~ 40 days after mycelia walks, continues After-mature cultivation
35-40 days；
S3: deepening to mycelia color, and bacterium bag quality softens, and opens bag after visible ozonium, and sack is folded to 3- above charge level
5cm high removes old fungus block, moves on to mushroom room, supplements charge level moisture with sprayer, is kept for 20-22 DEG C of temperature, space humidity 90-
95%, gas concentration lwevel 3000-4000ppm restores to bleach to charge level mycelia, reduces temperature to 18-20 DEG C, it is small to carry out 4 daily
Shi Guangzhao (intensity 800Lx) stimulation, reinforces ventilation, former base is promoted to be formed, mushroom flower bud differentiation, when mushroom flower bud is close to sack, by temperature tune
Whole to 16-18 DEG C, suitably reduction space humidity, reduction illumination, reduce ventilation, promote fructification elongation growth；It opens after bag about
It 25 days, can be harvested when mushroom handle length reaches 10-12 ㎝ when mushroom lid diameter is up to 0.8 ㎝.
Claims (10)
1. a kind of preparation process of edible mushroom suspension fluid strain, which comprises the steps of:
Step (1): the preparation of solid original seed strain: solid pedigree seed culture medium is fitted into sack or plastic bottle, by sack or plastics
After solid Primary spawn primary surface in bottle flattens, using steam high-voltage sterilizing, after being cooled to 23 ~ 26 DEG C, access test tube is female
It is put into culturing room after kind, the temperature of culturing room is maintained at: 23 ~ 25 DEG C, air humidity is maintained at: 60 ~ 65%, gas concentration lwevel
It is maintained at: 3000 ~ 4000ppm and is protected from light culture, until obtaining solid original seed strain；
Step (2): sterile suspension preparation: taking a container, and toward addition water, suspending agent in container, wherein water and suspending agent
Ratio is 100:1 ~ 2, after being passed through steam heating 100 ~ 140 minutes, stops heating, is cooled to 23 ~ 30 DEG C to container to get sterile
Suspension；
Step (3): the preparation of suspension fluid strain: the solid original seed strain that step (1) obtains is moved on in container, in container
Sterile suspension mixing is to get suspension fluid strain, and wherein solid original seed strain and sterile suspension volume ratio are 1:20.
2. a kind of preparation process of edible mushroom suspension fluid strain according to claim 1, which is characterized in that in step (1)
The preparation method of the solid pedigree seed culture medium includes the following steps:
(1) pretreatment of raw material: sawdust, wheat bran are spare with 50 mesh sieving machine fine screens；
(2) ingredient: the wheat bran 20% after sawdust 78%, fine screen, sucrose 1%, gypsum 1% after taking fine screen obtain solid after mixing
Raw material solid material and water is mixed evenly to get solid pedigree seed culture medium, wherein the water content of solid pedigree seed culture medium
60 62%.
3. a kind of preparation process of edible mushroom suspension fluid strain according to claim 1, which is characterized in that in step (1)
The steam high-voltage sterilizing refers to 2 2.5h of steam sterilizing when temperature is 121 DEG C.
4. a kind of preparation process of edible mushroom suspension fluid strain according to claim 1, which is characterized in that the suspending agent
Any one in low molecule suspending agent, polymeric retention aid suspension, silicic acid class suspending agent.
5. a kind of preparation process of edible mushroom suspension fluid strain according to claim 4, which is characterized in that the low molecule
Suspending agent includes glycerol, syrup；The polymeric retention aid suspension includes mucialga of arabic gummy, agar, methylcellulose, polyvinyl alcohol.
6. a kind of preparation process of edible mushroom suspension fluid strain according to claim 4, which is characterized in that the silicic acid class
Suspending agent includes alumina silicate, diatomite.
7. a kind of preparation process of edible mushroom suspension fluid strain according to claim 1, which is characterized in that in step (2)
Any one of the container in triangular flask, shaking flask, fermentor.
8. it is a kind of by any preparation method of claim 1-7 edible mushroom preparation field application.
9. application according to claim 8, which is characterized in that the preparation method of the edible mushroom the following steps are included:
S1: aseptically, aseptic gas is accessed in container described in step (3), the container inner pressure is maintained at
Between 0.02 ~ 0.05Mpa, and then the suspension fluid strain in container is accessed into cultivation package；
S2: moving into culture room for inoculated cultivation package, and culture room temperature is maintained at 23 ~ 25 DEG C, and air humidity is maintained at 65 ~
70%, gas concentration lwevel is maintained at 3000 ~ 4000ppm, is protected from light culture 35 ~ 40 days until mycelia walks, continuation After-mature cultivation
35-40 days；
S3: the cultivation package after mycelia is walked moves on to mushroom room, opens cultivation package, and the temperature of mushroom room is maintained at 20 ~ 22 DEG C, sky
Air humidity degree is maintained at 90 ~ 95%, and gas concentration lwevel is maintained at 3000 ~ 4000ppm；
S4: bleaching to charge level mycelia, reduces temperature to 18 ~ 20 DEG C, carries out 4 hours light stimulations, stronger ventilation amount daily；
S5: after mycelia grows up to mushroom flower bud, temperature is adjusted to 16 ~ 18 DEG C, and air humidity is maintained at 60 ~ 70%, daily illumination 4 hours,
Reduce ventilation quantity；
S6: it after mushroom flower bud grows up, can harvest.
10. application according to claim 8, which is characterized in that the preparation method of the edible mushroom the following steps are included:
S1: aseptically, aseptic gas is accessed in container described in step (3), the container inner pressure is maintained at
Between 0.02 ~ 0.05Mpa, and then the suspension fluid strain in container is accessed into cultivation package；
S2: moving into culture room for inoculated cultivation package, and culture room temperature is maintained at 23 ~ 25 DEG C, and air humidity is maintained at 65 ~
70%, gas concentration lwevel is maintained at 3000 ~ 4000ppm, is protected from light culture 35 ~ 40 days until mycelia walks, continuation After-mature cultivation
35-40 days；
S3: the cultivation package after mycelia is walked moves on to mushroom room, restores 2-3 days at a temperature of 22-24 DEG C, air humidity is maintained at
85-90%, gas concentration lwevel are maintained at 1500 ~ 2000ppm, carry out illumination in 4 hours daily, and add water moisturizing on ground；
S4: 2-3 days after removal cultivation package lantern ring, when mushroom flower bud is close to sack, should carry out thin flower bud in time, when mushroom handle length up to 10 ~
12cm, mushroom lid diameter reach 4cm, and edge thinning can harvest when opening a business open and flat.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910359170.1A CN109964736A (en) | 2019-04-30 | 2019-04-30 | A kind of preparation process and its application of edible mushroom suspension fluid strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910359170.1A CN109964736A (en) | 2019-04-30 | 2019-04-30 | A kind of preparation process and its application of edible mushroom suspension fluid strain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109964736A true CN109964736A (en) | 2019-07-05 |
Family
ID=67087240
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910359170.1A Pending CN109964736A (en) | 2019-04-30 | 2019-04-30 | A kind of preparation process and its application of edible mushroom suspension fluid strain |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109964736A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110495348A (en) * | 2019-07-11 | 2019-11-26 | 永富饶（天津）农业科技发展有限公司 | A kind of preparation method of planting almond abalone mushroom material |
| CN111955286A (en) * | 2020-08-28 | 2020-11-20 | 平泉市希才应用菌科技发展有限公司 | Preparation method of edible fungus liquid strain |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201127201Y (en) * | 2007-12-13 | 2008-10-08 | 浙江大学 | Steam type edible fungus inoculator |
| CN102550284A (en) * | 2011-12-31 | 2012-07-11 | 浙江大学 | Edible mushroom culturing method |
| CN102715014A (en) * | 2012-06-25 | 2012-10-10 | 浙江常山荣昇食用菌有限公司 | Preparation method for liquefied strain of edible mushroom |
| CN104054513A (en) * | 2014-07-17 | 2014-09-24 | 宋显和 | Cordyceps militaris strain cultivation method |
| CN104557209A (en) * | 2015-01-20 | 2015-04-29 | 浙江大学 | Strain culture medium for pleurotus eryngii liquefaction and corresponding culture method |
| CN104718995A (en) * | 2015-04-13 | 2015-06-24 | 青岛华盛绿能农业科技有限公司 | Method for producing solid liquefied strains of oyster mushrooms |
| CN104798602A (en) * | 2015-05-14 | 2015-07-29 | 湖南和平生物科技有限公司 | Industrialized production method of pleurotus eryngii |
| CN104838886A (en) * | 2015-04-29 | 2015-08-19 | 吴中区胥口精益生物医药研究所 | Industrial edible fungi cultivation method |
-
2019
- 2019-04-30 CN CN201910359170.1A patent/CN109964736A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201127201Y (en) * | 2007-12-13 | 2008-10-08 | 浙江大学 | Steam type edible fungus inoculator |
| CN102550284A (en) * | 2011-12-31 | 2012-07-11 | 浙江大学 | Edible mushroom culturing method |
| CN102715014A (en) * | 2012-06-25 | 2012-10-10 | 浙江常山荣昇食用菌有限公司 | Preparation method for liquefied strain of edible mushroom |
| CN104054513A (en) * | 2014-07-17 | 2014-09-24 | 宋显和 | Cordyceps militaris strain cultivation method |
| CN104557209A (en) * | 2015-01-20 | 2015-04-29 | 浙江大学 | Strain culture medium for pleurotus eryngii liquefaction and corresponding culture method |
| CN104718995A (en) * | 2015-04-13 | 2015-06-24 | 青岛华盛绿能农业科技有限公司 | Method for producing solid liquefied strains of oyster mushrooms |
| CN104838886A (en) * | 2015-04-29 | 2015-08-19 | 吴中区胥口精益生物医药研究所 | Industrial edible fungi cultivation method |
| CN104798602A (en) * | 2015-05-14 | 2015-07-29 | 湖南和平生物科技有限公司 | Industrialized production method of pleurotus eryngii |
Non-Patent Citations (6)
| Title |
|---|
| 侯祥保等: "白色真姬菇工厂化栽培技术要点 ", 《食用菌》 * |
| 杨淑云: "西北地区杏鲍菇工厂化栽培技术要点 ", 《北方园艺》 * |
| 王培凤等: "杏鲍菇规模化生产关键技术", 《现代农业科技》 * |
| 蔡建林等: "南方地区杏鲍菇工厂化高产袋栽技术 ", 《食药用菌》 * |
| 虞和澍: "平菇生产工艺改进 ", 《成都大学学报(自然科学版)》 * |
| 邹积华等: "无公害食品真姬菇工厂化生产技术规程", 《山东农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110495348A (en) * | 2019-07-11 | 2019-11-26 | 永富饶（天津）农业科技发展有限公司 | A kind of preparation method of planting almond abalone mushroom material |
| CN111955286A (en) * | 2020-08-28 | 2020-11-20 | 平泉市希才应用菌科技发展有限公司 | Preparation method of edible fungus liquid strain |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102144497B (en) | Process for planting pleurotus eryngii | |
| CN102265754B (en) | Pleurotus eryngii factory bottle cultivation method | |
| CN102523917B (en) | Method for cultivating straw mushroom | |
| CN102057836B (en) | Method for quickly producing edible fungus liquid strain by utilizing primary-secondary type culture tank | |
| CN109757307A (en) | Xianggu mushroom strain and its industrial planting method suitable for factory culture | |
| CN101773052A (en) | Preparation and industrial culture of aweto strain | |
| CN103270887B (en) | Silkworm chrysalis northern Chinese caterpillar Fungus industrial cultivation technique | |
| CN108522153A (en) | A method of utilizing liquid spawn fast-propagation hickory chick cultigen | |
| CN102612994B (en) | Method for cultivating oyster mushroom cultivation by directly mixing liquid spawns with raw materials | |
| CN104541938A (en) | Merge-cultivating method of Ganoderma | |
| CN101874453B (en) | Culture method of snow white mushroom strains and sporocarps | |
| CN104761386B (en) | The compost and preparation method of a kind of utilization giant knotweed rhizome residue Xinbao mushroom culturing | |
| CN104798602A (en) | Industrialized production method of pleurotus eryngii | |
| CN109220545A (en) | Using pine cedar sawdust as the Sparassis crispa cultivation matrix of raw material and its Sparassis crispa cultural method | |
| CN101897270A (en) | Production technology of Cordyceps sinensis mycelium | |
| CN110249912A (en) | A kind of method that Phellinus industrial bottle is planted | |
| CN109964736A (en) | A kind of preparation process and its application of edible mushroom suspension fluid strain | |
| CN108934785A (en) | A kind of the strain cultivation method and cultural method of Boletus aereus | |
| CN111386963A (en) | Tremella aurantialba cultivation method using tremella aurantialba seedling inoculation | |
| CN101720626B (en) | Culture medium formula of edible fungus second class fungus (protospecies) and making method | |
| CN102835310A (en) | Double-tray combined type plant tissue culture container | |
| CN107980474A (en) | A kind of cultural method of needle mushroom | |
| CN102786334A (en) | Culture medium for culturing edible fungus production mother seeds | |
| CN107517737B (en) | A kind of continuous quick bacteria stick manufacture craft of edible mushroom | |
| CN104285677B (en) | A kind of preparation method of edible mushroom peg wood bacterial classification |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190705 |