CN1850963A - Microbial culture system and microbial culture method using same - Google Patents
Microbial culture system and microbial culture method using same Download PDFInfo
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- CN1850963A CN1850963A CN 200610057145 CN200610057145A CN1850963A CN 1850963 A CN1850963 A CN 1850963A CN 200610057145 CN200610057145 CN 200610057145 CN 200610057145 A CN200610057145 A CN 200610057145A CN 1850963 A CN1850963 A CN 1850963A
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Abstract
The invention relates to an animalcule cultivating system that includes cultivating kettle, water process device, gas filling whisking device, and automatic control device. The heating device is set at the bottom of the kettle to heating one in three or one in four volume of the cultivating medium. The invention also discloses an animalcule cultivating system that cultivates in the cultivating device. The invention sharply decreases the energy and water consumption.
Description
Technical field
The present invention relates to a kind of new microorganism culturing system and use the method that this system carries out microorganism culturing, concrete, the new microorganism culturing system of the present invention comprises the improvement to microorganism culturing jar and auxiliary facility thereof, this system is applicable to the culture systems of the active bacteria formulation that uses in the processes such as plantation, breed, sewage disposal, can be used for cultivating anaerobism, different strain such as aerobic.Equipment of the present invention and method can be used for the cultivation of field microbial preparations such as various breed (livestock and poultry cultivation, aquaculture), plantation, water body purification of aquaculture, sewage disposal.
Background technology
Along with the development of microbial technique, microbial preparation has been applied in fields such as plantation, breed, sewage disposal more and more.Conventional plant cultured microorganism preparation cost is higher, and the biological activity that is hard to keep, and therefore, the localized culture technique of microorganism is arisen at the historic moment.Wherein, the microorganism culturing device is one of localized culture technique of microorganism.But,, very strict, therefore higher to the design requirements of microorganism culturing device to the requirement of assorted bacterium control and culture condition owing to strain properties differs.
At present existing multiple microorganism culturing device, as application number is that 00107619.1 Chinese patent application discloses a kind of little ecologic reactor, can only do single anaerobically fermenting, can not do complicated more microbial reaction cultivates, its cultivation only be single anaerobic species, and can not carry out the cultivation of tonifying Qi and illumination aspect.
The patent No. is that 00201168.9 Chinese patent discloses a kind of microorganism reactor, what take is direct-fired mode in tank body, there is not laminated heat-preserving, very simple on heating function and heat insulation function, this is in culturing process, and temperature variation is too fast, influence culture effect, and the direct heating intensification in tank body of intensification pipe can cause local temperature too high, thereby direct kill microorganisms influences culture effect.
The patent No. is that 02289164.1 Chinese patent discloses a kind of microorganism culturing device, though improving to some extent aspect heating and the insulation, from the effect of reality, many defectives is arranged still:
At first because its sterilization method for simply directly heating at a few hectolitre liquid in the jar, consumes a large amount of electric energy in this heat sterilization process, in the temperature-fall period after the sterilization process end, employing be cooling water temperature, consume a large amount of water.Concerning the user, use cost is high, bears very heavyly, very uneconomical, and therefore, this has offset the low-cost advantage that the microbial preparation localized production brings to a great extent.And too high current consumption and water loss do not meet the needs of building a conservation-minded society that country proposes yet.
Secondly, the stirring pump in that tank deck directly installs additional is provided with owing to be inverted, and is very easy to cause the interior lubricated greasy dirt of pump to reveal into tank body, and the culture environment of destroy microorganisms influences culture effect.Simultaneously, the stir mode of oar blade type, the cyclic balance between can not fine solution levels different densities material, influence is stirred and culture effect.
The 3rd, it is heated and is water conduction heating mode, can produce incrustation scale in the interlayer tank skin after duration of service is long, influences heating efficiency, increases energy consumption.Because tank body sealing, incrustation scale can not obtain cleaning, and influence the work-ing life of incubator greatly.
The 4th, carry out at the same space because it adds gentle cooling, turning in the temperature-fall period, at first will inject water coolant in a large number and add the hot water temperature with cooling well heater and reduction by heating, could reduce the temperature of jar interior substratum then, this process additive decrementation too much water resources and heat energy.
At last, it has increased the complexity of jar inner structure at the aerating pipeline that tank interior is provided with, and forms the corner or spot as yet untouched by a clean-up campaign easily, and easy cleaning does not influence culture effect.
Generally speaking, in present microorganism culturing technology and equipment, common employed equipment and method are heating, sterilization, cooling and the microorganism culturing of carrying out substratum in same equipment, therefore just need at first equipment to be heated to realize the sterilization of substratum, must make total system be cooled to the required temperature of microorganism culturing by cooling system afterwards.In above-mentioned heating or temperature-fall period, will inevitably consume a large amount of energy and a large amount of water.The problems referred to above in the microorganism culturing technology have become the microorganism culturing technical development and have reduced the bottleneck of production energy consumption and production cost.
The inventor has carried out research for many years to microbial fermentation technology and equipment, has designed novel fermentation equipment, in order to overcome the many disadvantages that exists in the prior art, has not only improved fermentation equipment, also makes fermentation technique obtain significant progress.
Summary of the invention
The purpose of this invention is to provide a kind of microorganism culturing system new, energy-saving and water-saving type, concrete, the new microorganism culturing system of the present invention is the improvement to existing microorganism culturing jar and auxiliary facility thereof.Be applied to this system and can when improving the microbial preparation training quality, reduce production energy consumption greatly.
Concrete, the invention provides a kind of microorganism culturing system, this system comprises: the culture tank that is used for heating, sterilization, cooling and the microorganism culturing of substratum; Be used to prepare the water treating equipment that meet the microbial cultivation process water quality requirement; Stir aerating equipment and automatic control equipment, it is characterized in that the heating installation of described culture tank is positioned at the bottom of tank body, be used to heat the substratum that has only tank body volume 1/3rd to 1/4th in the tank body; Described stirring aerating equipment is recycle pump.
Concrete, described culture tank is used for heating, sterilization, cooling and the microorganism culturing of substratum.In microorganism culturing of the present invention system, described culture tank comprises tank body and heating installation, and described heating installation is positioned at the bottom of tank body, is used for the tank body heating; Be provided with at the top of tank body observe handle hole, batching hole, with the hole that water treating equipment and stirring aerating equipment are connected, be provided with discharge hole in the bottom of tank body.
Described water treating equipment are connected with described culture tank by pipeline, are used for the required water of microorganism culturing is handled, and make it to meet the water quality requirement of culturing process.
Described stirring aerating equipment comprises recycle pump and airing system, and described recycle pump can be the pump that has circulation simultaneously and add airway dysfunction, for example senior sanitary pump, and it adds tolerance is 90L/h-300L/h; Described recycle pump comprises gas suction device and gas filter, when being used to need aerating, sucks and the needed gas of filtration, purification.Described stirring aerating equipment is connected with described culture tank by pipeline, by adding the gas that does not influence microorganism culturing the liquid in the described culture tank is stirred when recurrent state, makes the liquid thorough mixing in the described culture tank.Described stirring aerating equipment has adopted recycle pump, and the application cycle pump can make whole material system be stirred fully by the circulation of material, and blended is even especially.When carrying out the cultivation of anaerobism mushroom, can only use this equipment to stir, but not feed gas, or feed the gas that does not influence microorganism culturing, for example nitrogen or carbon dioxide etc.; When carrying out the cultivation of aerobic mushroom, can use the circulation of this equipment to stir and aerating oxygen is with delivery of supplemental oxygen tolerance simultaneously, perhaps aerating oxygen only carries out air agitation in supplemental oxygen, aerobic nutrient solution in the jar is carried out oxygenation cultivate.
Described automatic control equipment and culture tank, water treating equipment, stirring aerating equipment electrically connect, and are used to control heat-processed, culturing process, water treatment procedure and the stirring aerating process of culture tank.
Further, in microorganism culturing of the present invention system, described culture tank comprises tank body and heating installation, and described heating installation is positioned at the bottom of tank body, is used for the tank body heating; Be provided with at the top of tank body observe handle hole, batching hole, with the hole that water treating equipment are connected with whipping device, be provided with discharge hole in the bottom of tank body.The sidewall of described tank body also is provided with the insulation interlayer, and, being provided with the cooling coil pipe in insulation interlayer inside, the upper end of cooling coil pipe sets out the mouth of a river, and the lower end is provided with water-in.Wherein said heating installation can be the heating installation of this area routine, for example electrical heating device.
Further, in microorganism culturing of the present invention system, also can offer one or more viewing windows, be used to observe the position of tank body liquid at the sidewall of described tank body.
Further, in microorganism culturing of the present invention system, described water treating equipment comprise water sterilization parts, purifying part and/or water correction parts, and an end of described sterilization parts, purifying part or water correction parts connects the water source, and the other end connects culture tank; Described sterilization parts, purifying part and water correction parts are connected successively, and the sterilization parts connect the water source, are connected culture tank with the water correction parts.More specifically, wherein said sterilization parts are the ultraviolet disinfection parts; Purifying part is one or more levels filter element; The water correction parts are dechlorination equipment and/or water demineralization equipment.
Further, in microorganism culturing of the present invention system, described aerating whipping device comprises gas tank and recycle pump, and the inlet mouth of described recycle pump is connected with the bottom of culture tank by pipeline, and the air outlet of described recycle pump is connected with the top of culture tank by pipeline.More specifically, described recycle pump also comprises gas suction device and gas filter, and described gas filter is used at described whipping device the gas that is sucked being filtered, to remove impurity and the assorted bacterium in the gas when adding gaseity.
Further, in energy-saving and water-saving type microorganism culturing of the present invention system, described automatic control equipment comprises:
Data acquisition module is used to gather the temperature of culture tank;
Central processing module is used to accept the temperature data that described data acquisition module sends, and the connecting inner program stored, sends execution command;
Execution module is used to receive the execution command that described central processing module sends, and culture tank is started or stoped heating, opens or close water treating equipment, starts or stops whipping device.
More specifically, in described energy-saving and water-saving type microorganism culturing system, described data acquisition module comprises the intravital temperature sensor of jar that is positioned at culture tank; Described central processing module comprises micro-chip or programmable logic controller or computer; Described execution module is positioned at the intravital regenerator of jar of culture tank, various valve and/or electric machine controller.
In order to be well understood to the principle of microbial cultivation equipment of the present invention, can be referring to Fig. 1, Fig. 1 is the schematic diagram of microbial cultivation equipment of the present invention.Microbial cultivation equipment of the present invention comprises culture tank 1, water treating equipment 2, and whipping device 3, automatic control equipment 4, described culture tank 1 is used for heating, sterilization, cooling and the microorganism culturing of substratum; Described water treating equipment 2 are connected with described culture tank 1 by pipeline, are used for the water of substratum cooling is handled, and make it to meet the water quality requirement of culturing process; Described whipping device 3 is connected with described culture tank 1 by pipeline, is used for the liquid in the described culture tank 1 is stirred, and makes the liquid thorough mixing in the described culture tank 1; Described automatic control equipment 4 electrically connects with culture tank, water treating equipment, whipping device, is used to control heat-processed, water treatment procedure and the whipping process of culture tank.
In microbial cultivation equipment of the present invention, the heating installation of tank body is only at 1/3rd to 1/4th substratum heating in the tank body, so temperature increase is rapid, and energy consumption reduces greatly, can adapt to the Rural areas use of vast unpowered electricity.Adopt the deep fat type of heating in addition, because deep fat boiling point height, so heat conduction efficiency improves greatly, and heat-up rate is accelerated, and the thermosteresis in the heat sterilization process reduces, and also helps save energy.According to measuring and calculating, this equipment is 1/4th to 1/5th of traditional microorganism culturing device current consumption.
In the temperature-fall period of microbial cultivation equipment of the present invention, because the aseptic water body of normal temperature is 2-3 times of the sterilising medium volume, when therefore the sterilized water after filtering injected, the pot liquid temperature was reduced to about culture temperature naturally, need not to carry out extra cooling operation in this process.When if cooling back temperature can not reach accurately requiring of meeting, when summer, water temperature was higher, can just can arrive culture temperature by a small amount of water coolant of annotating to the cooling coil pipe, therefore this equipment can not use water coolant basically, or only uses water coolant seldom can satisfy the needs that substratum is lowered the temperature.
In microbial cultivation equipment of the present invention, adopt the conventional stirring pump that directly installs additional at tank deck, determining of this alr mode is owing to stir pump and be inverted and be provided with, and is very easy to cause in the pump lubricated greasy dirt to reveal into tank body, the culture environment of destroy microorganisms influences culture effect; Simultaneously, the stir mode of oar blade type, the cyclic balance between can not fine solution levels different densities material, influence is stirred and culture effect.The present invention adopts and stirs aerating equipment, and this equipment comprises recycle pump and airing system, and described recycle pump can make a jar interior material fully be stirred in recurrent state, makes the liquid thorough mixing in the described culture tank.In needing the culturing process of aerating, aerating also can produce stirring action to nutrient solution.
In sum, the present invention has carried out following improvement with traditional microbial cultivation equipment and method: (1) changes the simple heat sterilization mode of substratum the heat sterilization of part substratum into and the asepticize of water is handled two processes, greatly reduces the consumption of electric energy and water coolant; (2) traditional mechanical stirring mode is changed into pump circulation aerating mode, improved stirring efficiency and the dissolved oxygen efficiency when cultivating aerophil greatly.After doing above-mentioned improvement, microbial cultivation equipment of the present invention need not to use 380 volts power electricity consumption, gets final product with common civil power.Because energy consumption, water consumption reduce, and less demanding to electric load, and therefore very big popularization space is arranged.When using it for fodder production, the preparation of its production can help peasant household to reduce the use of medicine, produces the animal products that meets ecological requirement, greatly improves raiser's culture benefit.
Another object of the present invention has provided a kind of new microbial culture method, this method comprises that using microorganism culturing of the present invention system carries out the cultivation of heating, sterilization and the microorganism of substratum, and it mainly is that water by adding normal temperature is realized that the cooling that the method is characterized in that the substratum behind the heat sterilization is handled; And do not stir in the culturing process or stir by aerating.
The invention provides a kind of microbial culture method, this method comprises:
1, the heat sterilization of substratum;
2, the cooling of substratum is handled; With
3, carry out microorganism culturing according to the need culture temperature and the time of cultivation bacterial classification;
It is characterized in that described microorganism culturing is to carry out in the microbial cultivation equipment mentioned above.
Concrete, microbial culture method of the present invention comprises:
1, the heat sterilization of substratum
Microbiological culture media is added the culture tank of microorganism culturing of the present invention system, be heated to sterilising temp and keep this temperature regular hour, to finish the sterilization of substratum;
2, the cooling of water treatment and substratum is handled
Water is handled through water treatment system of the present invention, made it to meet the culturing process water quality requirement.
The water that meets the culturing process water quality requirement a certain amount of normal temperature, that handle through water treatment system is added culture tank, make that the temperature of substratum drops to the temperature that meets cultivation needs substantially in the jar, heat slightly or the processing of lowering the temperature just can reach specified culture temperature; With
3, carry out microorganism culturing according to the need culture temperature and the time of cultivation bacterial classification.
More specifically, microbial culture method of the present invention comprises:
1, the heat sterilization of substratum
Microbiological culture media is added the culture tank of microorganism culturing of the present invention system, and its volume is 1/3rd to 1/4th of a tank body volume, is heated to sterilising temp and keeps sterilising temp to sterilize the required time, to finish the sterilization of substratum;
2, the cooling of water treatment and substratum is handled
Water is handled through water treatment system of the present invention, made it to meet the culturing process water quality requirement.Water is carried out disinfecting action by ultraviolet disinfection equipment; Enter strainer then and carry out three-stage filtration, remove impurity and remaining bacteria in the water; Enter water correction device at last and carry out water correction, to overcome different areas water quality difference; The water quality requirement that meets culturing process through the water after the above step process fully;
The water that meets culturing process water quality requirement normal temperature, that handle through water treatment system is added culture tank, the volume of above-mentioned water is the twice to three times of culture volume, the temperature of mixing liquid in the jar of back drops to the temperature that meets microorganism culturing substantially, for example about 35 degree, heat slightly or lower the temperature to handle and just can reach specified culture temperature, if temperature is still too high, or leaves standstill cooling or adjust by the inner cooling coil pipe that is provided with of tank body insulation interlayer; With
3, constant temperature is cultivated
After mixed fluid temperature arrives culture temperature, to the inoculation of medium bacterial classification, after fully stirring, specified culture temperature is set by controller, this moment, incubator entered automatic culturing process, till the incubation time that system is provided with; In culturing process, can optionally stir or the aerating operation.
Because method of the present invention changes the heat sterilization process of substratum the heat sterilization of part substratum into and the asepticize of water is handled two processes, greatly reduces the consumption of electric energy and water coolant; And this method changes traditional mechanical stirring mode into pump circulation (aerating) mode, has improved stirring efficiency and the dissolved oxygen efficiency when cultivating aerophil greatly.After doing above-mentioned improvement, microbial culture method energy consumption of the present invention, water consumption reduce, and less demanding to electric load, and therefore very big popularization space is arranged.When using it for microorganism feed addictive production, will greatly improve raiser's culture benefit.
Method of the present invention can be applicable to the cultivation of field microbial preparations such as various breed (livestock and poultry cultivation, aquaculture), plantation, water body purification of aquaculture, sewage disposal, particularly is applicable to the large-scale cultivation field, cultures sub-district, growing vegetables district, sewage work etc.Each organ dysfunction of livestock and poultry body can be adjusted and improve to its preparation, increase immunizing power, improve feed conversion, thereby promote growth of animals or poultry and breeding, raising survival rate, prevent and cure diseases, improve animal products quality, purifying aquaculture environment, increase economic efficiency aspect effect obvious.
Below in conjunction with accompanying drawing and specific embodiment technical scheme of the present invention is described in detail.
Description of drawings
Fig. 1 is the schematic diagram of microbial cultivation equipment of the present invention;
Fig. 2 is the structural representation of an embodiment of microbial cultivation equipment of the present invention;
Fig. 3 is the synoptic diagram of the vertical view of Fig. 2;
Fig. 4 is the automatic control equipment schematic diagram of an embodiment of microbial cultivation equipment of the present invention.
Embodiment
Below by concrete embodiment technical scheme of the present invention is further described, the embodiment that wherein exemplifies only is to explanation of the present invention, and can not limit protection scope of the present invention by any way.
Fig. 2 and Fig. 3 are for an embodiment of microbial cultivation equipment of the present invention, and in the present embodiment, described culture tank 1, water treating equipment 2, whipping device 3 and automatic control equipment 4 support by support 5, and water treating equipment 2 are connected with the water source by pipeline 6.
Described culture tank 1 comprises tank body 11 and zone of heating 12, and described zone of heating 12 is positioned at the bottom of tank body 11, adds oil in this zone of heating 12, by well heater 121 oil is heated, by this deep fat is tank body 11 heating, in order to observe the position of oil, also is connected with oil level at this zone of heating 12 and observes meter 122; Bottom at tank body 11 is provided with discharge hole, by connected discharge nozzle 16 liquid of tank body 11 inside is discharged, and as for when discharging, realizes by the valve of controlling on this discharge nozzle 16 161.
Be provided with the hole 15 of observing handle hole 13, batching hole 14, being connected with whipping device with water treating equipment at the top of tank body 11, this hole 15 is connected with whipping device 3 with water treating equipment 2 respectively by pipeline, and on the pipeline that connects water treating equipment 2 and whipping device 3, be provided with valve, be used to control being connected of culture tank 1 and water treating equipment 2 or whipping device 3.In the present embodiment, water treating equipment 2 are connected with culture tank 1 with whipping device 3 shared holes 15, can certainly be connected with culture tank 1 by different holes, do not repeat them here.
In addition, also be provided with respiratory organ 17 at the top of tank body 11, head light 18.
The sidewall of described tank body 11 is provided with insulation interlayer 110, and, being provided with cooling coil pipe 111 in insulation interlayer 110 inside, the upper end of cooling coil pipe sets out the mouth of a river 1111, and the lower end is provided with water-in 1110.
In order to observe the position of tank body 11 internal liquids, offer one or more viewing windows at described tank wall, as the viewing window in the present embodiment 112,113, wherein, viewing window 112 is used to observe the position of the mixing liquid of the substratum of adding and water in tank body 11 inside; Viewing window 113 is used to observe and adds through the position of the mixing liquid behind the water coolant after the water treatment in tank body 11 inside.
Water treating equipment 2 generally comprise water sterilization parts, purifying part and water correction parts, perhaps have only one or several combination arbitrarily in the above-mentioned parts.In the present embodiment, three kinds of above-mentioned parts have been comprised.Wherein, described sterilization parts are ultraviolet disinfection parts 21; Purifying part is the three-stage filtration parts, i.e. one-level filter element 22, cascade filtration parts 23, secondary filter parts 24; Water correction parts 25 are dechlorination equipment and water demineralization equipment.One end of ultraviolet disinfection parts 21 connects the water source, and the other end connects one-level filter element 22, cascade filtration parts 23, secondary filter parts 24, water correction parts 25 successively, and water correction parts 25 connect culture tank 1 by pipeline.When water is handled, open ultraviolet disinfection parts 21 and be connected valve 26 with the water source, water passes through the sterilization of ultraviolet disinfection parts 21 successively, the filtration of one-level filter element 22, cascade filtration parts 23, secondary filter parts 24, because the water quality of each department may contain different ions, for reaching the water quality requirement of substratum, need water correction through water correction parts 2.In the time need cooling off to the substratum in the culture tank 1, open valve 27 and 151, like this, the treated satisfactory water in the water treating equipment 2 just enters the tank body 11 of culture tank 1 along pipeline, mix with substratum, to its cooling, cooling.
In addition, the recycle pump 31 among the present invention also comprises gas suction device (not shown) and gas filter, has the suction to gas, filtering function.Like this, the present invention can also be used for the cultivation that some need certain gas bacterium.When aerating, by gas filter the gas that adds is filtered, remove impurity, assorted bacterium etc.In the present invention, gas filter is an air filter 32, and traditional aerating mode just simply is input to tank interior with air by pipeline, and the dissolved oxygen efficiency of this mode is lower, and a jar interior aerating pipeline is very easy to form the not corner or spot as yet untouched by a clean-up campaign of easy cleaning.The present invention adopts the aerating mode in recycle pump internal recycle whipping process, has strengthened the contact surface and the contact pressure of empty G﹠W, so the dissolved oxygen effect improves greatly, and effect is very remarkable when cultivating aerophil.
For the tank body to culture tank 1 cleans, in the sequence of control of automatic control equipment 4, be provided with cleaning function, the pipeline 6 that is connected with the water source communicates by the tank body of valve 61 with culture tank 1, in tank body, be provided with a rotating wash bowl 62, when needs clean, close water treating equipment 2 and whipping device 3, open valve 61, water from pipeline 6 enter the tank interior of culture tank 1, clean by 62 pairs of tank interior of rotating wash bowl, after having cleaned, open the valve 161 of the discharge nozzle of culture tank 1 bottom, sewage is discharged.The present invention cleans thoroughly, and is easy to operate.
The cultivation of embodiment 2 probiotic bacterium yeast saccharomyces cerevisiaes
1, in the culture tank of 300 liters of useful volumes, add substratum from the handle hole at tank body top, described substratum is glucose 3kg, ammonium sulfate 1.5kg, yeast extract paste 1.5kg, sal epsom 0.9kg, Sodium phosphate dibasic 1kg, potassium primary phosphate 0.2kg and water, and cumulative volume is about 80 liters;
2, open machine power, press the control panel cocycle and stir switch, when the substratum in the jar is heated to 95 ℃, stops heating, and kept this temperature 30 minutes;
3, fetch boiling water refining plant and be connected to the pipeline valve at tank body top is connected the water purification system pipeline with water pipe, open the water source, water quality through purification add after the degerming jar in, adding volume is that cumulative volume is reached about 300 liters; This moment, the temperature of liquid was about 27 ℃, opened the cooling pipeline valve, was connected with the water source, opened the water source, pot liquid is lowered the temperature, and be about 25 ℃ up to temperature;
4, open each valve that recycle pump is connected with tank body, open the recycle pump switch, make the pot liquid thorough mixing;
5, operation room and tool using are carried out disinfection after the sterilization, open tank body top batching hole, in yeast saccharomyces cerevisiae solid spawn 100g was added jar, living bacteria count was 1.0 * 10
10Cfu/ml covers tight lid;
6, open the cultivation key, open recycle pump and oxygenation functional switch, nutrient solution is stirred oxygenation cultivate, incubation time is 24 hours;
7, after to be recycled the stirring, close the recycle pump switch, open bottom discharge port valve, emit preservation cultivating the liquid that finishes in the jar.
8, open the rotating wash bowl valve, the source switch of fetching boiling water is to cleaning in the jar.
Obtain 300 liters of live yeast liquid mediums thus, its effective viable count is 1.0 * 10
9Cfu/ml.This nutrient solution is mainly used in and is used for aquaculture, behind animal drinking-water, this nutrient solution of feeding, can regulate gastrointestinal tract of livestock and fowls function, enhance immunity power, improves food conversion ratio, improves breeding environment and reduce the chemicals usage quantity.
Above embodiment is only unrestricted in order to explanation the present invention, although the present invention is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement the present invention, and not breaking away from the spirit and scope of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (10)
1. microorganism culturing system, this system comprises: the culture tank that is used for heating, sterilization, cooling and the microorganism culturing of substratum; Be used to prepare the water treating equipment that meet the microbial cultivation process water quality requirement; Stir aerating equipment and automatic control equipment, it is characterized in that the heating installation of described culture tank is positioned at the bottom of tank body, be used to heat the substratum that has only tank body volume 1/3rd to 1/4th in the tank body; Described stirring aerating equipment is recycle pump.
2. microorganism culturing according to claim 1 system is characterized in that the sidewall of described culture tank tank body is provided with the insulation interlayer, at the inner optional cooling coil pipe that is provided with of insulation interlayer.
3. microorganism culturing according to claim 1 system, it is characterized in that described water treating equipment comprise water sterilization parts, purifying part and/or water correction parts, described sterilization parts, purifying part and water correction parts are connected successively, the sterilization parts connect the water source, are connected culture tank with the water correction parts; Wherein said sterilization parts are the ultraviolet disinfection parts; Described purifying part is one or more levels filter element; The water correction parts are dechlorination equipment and water demineralization equipment.
4. microorganism culturing according to claim 1 system, it is characterized in that described stirring aerating equipment comprises recycle pump and airing system, described recycle pump comprises gas suction device and gas filter, when being used to need aerating, sucks and the needed gas of filtration, purification.
5. microorganism culturing according to claim 1 system is characterized in that described automatic control equipment comprises:
Data acquisition module is used to gather the temperature of culture tank;
Central processing module is used to receive the temperature data that described data acquisition module sends, and the connecting inner program stored, sends execution command;
Execution module is used to receive the execution command that described central processing module sends, and culture tank is started or stoped heating, opens or close water treating equipment, starts or stops whipping device.
6. microbial culture method, this method comprises:
(1) heat sterilization of substratum;
(2) cooling of substratum is handled; With
(3) carry out microorganism culturing according to cultivating required culture temperature and the time of bacterial classification;
It is characterized in that described microorganism culturing carries out in the described microbial cultivation equipment of claim 1-5.
7. microbial culture method according to claim 6, this method comprises:
(1) heat sterilization of substratum
Microbiological culture media is added the culture tank of the described microorganism culturing of claim 1-5 system, be heated to sterilising temp and keep this temperature regular hour, to finish the sterilization of substratum;
(2) cooling of water treatment and substratum is handled
Water is handled through water treatment system of the present invention, made it to meet the microorganism culturing water quality requirement;
With a certain amount of natural temperature, add culture tank through water treatment system water that handle, that meet the microorganism culturing water quality requirement, make that the temperature of substratum drops to the temperature that meets the cultivation needs substantially in the jar; With
(3) carry out microorganism culturing according to cultivating required culture temperature and the time of bacterial classification.
8. microbial culture method according to claim 7, this method comprises:
(1) heat sterilization of substratum
The culture tank that microbiological culture media is added the described microorganism culturing of claim 1-5 system, the volume of described substratum is 1/3rd to 1/4th of a tank body useful volume, be heated to sterilising temp and keep sterilising temp to sterilize the required time, to finish the sterilization of substratum;
(2) cooling of water treatment and substratum is handled
Water is handled through water treatment system of the present invention, made it to meet the microorganism culturing water quality requirement;
With a certain amount of natural temperature, add culture tank through water treatment system water that handle, that meet the microorganism culturing water quality requirement, the volume of above-mentioned water is the twice to three times of culture volume, the temperature of mixing liquid in the jar of back drops to the temperature that meets microorganism culturing substantially, as deviation slightly, can heat or lower the temperature and handle to reach specified culture temperature; With
(3) constant temperature is cultivated
After mixed fluid temperature arrives culture temperature,, after fully stirring, under the culture temperature of setting, cultivate, till the needed incubation time of microorganism culturing to the inoculation of medium bacterial classification.
9. according to any described microbial culture method of claim 6-8, wherein also be included in the aerating of carrying out optionally optional in the culturing process and operate.
10. application rights requires any described microorganism culturing system of 1-5 or any described microbial culture method of claim 6-9 to produce the microbial preparation that various breed, plantation, water body purification of aquaculture, sewage treatment area are used.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100571450A CN100395326C (en) | 2006-03-10 | 2006-03-10 | Microbial culture system and microbial culture method using same |
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| CNB2006100571450A CN100395326C (en) | 2006-03-10 | 2006-03-10 | Microbial culture system and microbial culture method using same |
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| CN1850963A true CN1850963A (en) | 2006-10-25 |
| CN100395326C CN100395326C (en) | 2008-06-18 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559497A (en) * | 2011-12-22 | 2012-07-11 | 四川科伦药业股份有限公司 | Sterile preparation, filling method of culture medium and equipment |
| CN106190809A (en) * | 2016-07-25 | 2016-12-07 | 彭阳县福泰菌业有限责任公司 | Liquid spawn thermostatted water blood circulation |
| CN107779385A (en) * | 2016-08-25 | 2018-03-09 | 卓金星 | Manufacture system of fermenting and fermentation process |
| CN108456626A (en) * | 2018-03-22 | 2018-08-28 | 黄佳立 | A kind of bioengineering swing arm type microculture soil mixing arrangement |
| CN110468044A (en) * | 2019-09-06 | 2019-11-19 | 重庆沐联环境工程有限公司 | A kind of microorganism constant temperature incubation integration apparatus and application method |
| CN110684664A (en) * | 2019-12-02 | 2020-01-14 | 邯郸市正德节能环保有限公司 | Biological bacterium culture method |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE29520294U1 (en) * | 1995-12-21 | 1996-02-08 | Rubenberger Karl | Device for the propagation of microorganisms |
| JP2000325979A (en) * | 1999-05-19 | 2000-11-28 | Denso Corp | Filter medium for adsorbing sporulable microorganism, production thereof, bath water cleaning device including the filter medium and culture method of the microorganisms |
| CN1274750A (en) * | 2000-05-16 | 2000-11-29 | 沈泽江 | Integral micro-ecological reactor and its application method in making ecological bacterial preparation |
| CN2512786Y (en) * | 2001-10-30 | 2002-09-25 | 陶德录 | Animal micro ecological bactria cultivation bionic device |
| CN1483806A (en) * | 2002-09-20 | 2004-03-24 | 龙 栾 | Biological reactor for large scale culturing plant cell |
| CN2606110Y (en) * | 2002-12-11 | 2004-03-10 | 丁之铨 | Microbial culture garden |
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2006
- 2006-03-10 CN CNB2006100571450A patent/CN100395326C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559497A (en) * | 2011-12-22 | 2012-07-11 | 四川科伦药业股份有限公司 | Sterile preparation, filling method of culture medium and equipment |
| CN102559497B (en) * | 2011-12-22 | 2013-12-25 | 四川科伦药业股份有限公司 | Sterile preparation, filling method of culture medium and equipment |
| CN106190809A (en) * | 2016-07-25 | 2016-12-07 | 彭阳县福泰菌业有限责任公司 | Liquid spawn thermostatted water blood circulation |
| CN107779385A (en) * | 2016-08-25 | 2018-03-09 | 卓金星 | Manufacture system of fermenting and fermentation process |
| CN108456626A (en) * | 2018-03-22 | 2018-08-28 | 黄佳立 | A kind of bioengineering swing arm type microculture soil mixing arrangement |
| CN110468044A (en) * | 2019-09-06 | 2019-11-19 | 重庆沐联环境工程有限公司 | A kind of microorganism constant temperature incubation integration apparatus and application method |
| CN110684664A (en) * | 2019-12-02 | 2020-01-14 | 邯郸市正德节能环保有限公司 | Biological bacterium culture method |
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| Publication number | Publication date |
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| CN100395326C (en) | 2008-06-18 |
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