CN102559497B - Sterile preparation, filling method of culture medium and equipment - Google Patents
Sterile preparation, filling method of culture medium and equipment Download PDFInfo
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- CN102559497B CN102559497B CN 201110435063 CN201110435063A CN102559497B CN 102559497 B CN102559497 B CN 102559497B CN 201110435063 CN201110435063 CN 201110435063 CN 201110435063 A CN201110435063 A CN 201110435063A CN 102559497 B CN102559497 B CN 102559497B
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- substratum
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
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Abstract
The invention discloses a sterile preparation, filling method of culture medium and equipment, particularly comprising blowing compressed gas into the bottom of a culture medium preparation can to subject the culture medium to glass blowing stirring, blowing compressed gas into the upper part of the culture medium preparation can to serve as motive power after preparing the culture medium, discharging the culture medium out of the can. According to the sterile preparation, filling method of culture medium and equipment, the structure is simple, integrated and automatic process of the preparation and filling of the culture medium and standard operation are achieved, and pollution problem in the preparation and filling process is effectively solved.
Description
Technical field
The present invention relates to a kind of sterile preparation, packaging process and equipment of substratum.
Background technology
The at present configuration of substratum does not have clear and definite regulation, exist operating process arbitrarily, easy microbial contamination and occur that the substratum cultivating false positive, use in time mostly is a lot of shortcomings such as manual filling without corresponding sfgd., canning means.
Summary of the invention
The problem existed for prior art, but the object of the present invention is to provide the aseptic configuration of substratum of the never synthermal requirement of a kind of efficient solution simple in structure and filling, be applicable to sterile preparation, packaging process and the equipment of fairly large substratum.
For achieving the above object, the sterile preparation of a kind of substratum of the present invention, packaging process, be specially: be blown into the pressurized gas stir culture base of being blown in the bottom of substratum preparing tank, after preparing substratum, pass into pressurized gas as power on the top of substratum preparing tank, make substratum discharge tank body.
Further, described pressurized gas is pressurized air or compressed nitrogen up-to-standard after Sterile Filtration.
Further, described substratum adopts PLC to control the switching time of pneumavalve to reach the accurate control of loading amount at filling place.
A kind of sterile preparation, filling apparatus of implementing the substratum of aforesaid method, the filling dividing cell that comprises substratum preparing tank and precise control loading amount, between substratum preparing tank and filling dividing cell, by liquid-transport pipe-line, be communicated with, the top connection of substratum preparing tank is provided with the empty reducing valve of the pressure that the pressurized gas of discharge opeing power liquid-transport pipe-line is provided and controls the tank internal pressure.
Further, the top of described substratum preparing tank also is provided with the second liquid-transport pipe-line, has been arranged in parallel the first high-temperature steam pipeline and vacuum-pumping pipeline and corresponding control valve thereof on this second liquid-transport pipe-line.
Further, the bottom of described substratum preparing tank is provided with stirring gas and is blown into mouth.
Further, described substratum preparing tank comprises interior pot and outer tank body, be provided with airtight wall fully between interior pot and outer tank body, be provided with the 3rd liquid-transport pipe-line be connected with wall on outer tank body, be arranged in parallel the second high-temperature steam pipeline and cooling water pipeline and corresponding control valve thereof on the 3rd liquid-transport pipe-line.
Further, the bottom of described outer tank body is provided with water shoot and water discharge valve thereof.
Further, the provided upstream of described liquid-transport pipe-line is equipped with the preparing tank pneumavalve, and middle part is provided with strainer, and downstream is provided with filling pneumavalve.
Further, be provided with several filling isocons on described filling dividing cell, be provided with the time delay switch that adopts PLC to control on each filling isocon, this time delay switch is controlled the switching time of described filling pneumavalve to reach the accurate control of loading amount.
The present invention is simple in structure, has realized the preparation of substratum and filling integrated, automation process, can realize standardized work, has effectively solved the pollution problem in preparation and pouring process.
The accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Embodiment
Below, with reference to accompanying drawing, the present invention is more fully illustrated, shown in the drawings of exemplary embodiment of the present invention.Yet the present invention can be presented as multiple multi-form, and should not be construed as the exemplary embodiment that is confined to narrate here.But, these embodiment are provided, thereby make the present invention comprehensively with complete, and scope of the present invention is fully conveyed to those of ordinary skill in the art.
For ease of explanation, here can use such as " on ", the space relative terms such as D score " left side " " right side ", the relation for element shown in explanatory view or feature with respect to another element or feature.It should be understood that except the orientation shown in figure, spatial terminology is intended to comprise the different azimuth of device in using or operating.For example, if the device in figure is squeezed, be stated as the element that is positioned at other elements or feature D score will be positioned at other elements or feature " on ".Therefore, the exemplary term D score can comprise upper and lower orientation both.Device can otherwise be located (90-degree rotation or be positioned at other orientation), and the relative explanation in space used here can correspondingly be explained.
As shown in Figure 1, the sterile preparation of a kind of substratum of the present invention, packaging process, be specially: be blown into the pressurized gas stir culture base of being blown in the bottom of substratum preparing tank, after preparing substratum, pass into pressurized gas as power on the top of substratum preparing tank, make substratum discharge tank body.
In the present invention, pressurized gas is pressurized air or compressed nitrogen up-to-standard after Sterile Filtration, and substratum adopts PLC to control the switching time of pneumavalve to reach the accurate control of loading amount at filling place.
A kind of sterile preparation of substratum, filling apparatus, comprise the filling dividing cell 2 of substratum preparing tank 1 and precise control loading amount, between substratum preparing tank 1 and filling dividing cell 2, by liquid-transport pipe-line 3, is communicated with.
The top of substratum preparing tank 1 also is provided with on the second liquid-transport pipe-line 11, the second liquid-transport pipe-lines 11 and has been arranged in parallel the first high-temperature steam pipeline 12 and vacuum-pumping pipeline 13 and corresponding control valve thereof.Before the substratum preparation, need to carry out disinfection to tank interior integral body, sterilizing, by vacuumizing in 13 pairs of tank bodies of vacuum-pumping pipeline, import high-temperature gas by the first high-temperature steam pipeline 12 in tank body, all can reach the purpose of effective sterilization, simultaneously, in preparing the process of substratum, by vacuumizing in 13 pairs of tank bodies of vacuum-pumping pipeline, also can make substratum avoid microbial contamination, keep comparatively stable state, to reach the requirement of preparation condition.
Substratum preparing tank 1 comprises interior pot 4 and outer tank body 5, be provided with airtight wall 6 fully between interior pot 4 and outer tank body 5, be provided with the 3rd liquid-transport pipe-line 7 be connected with wall on outer tank body 5, the second high-temperature steam pipeline 8 and cooling water pipeline 9 and corresponding control valve thereof have been arranged in parallel on the 3rd liquid-transport pipe-line 7, the bottom of outer tank body 5 is provided with water shoot 10 and water discharge valve thereof, and the water of wall 6 interior storages can be discharged by water shoot 10.In the process of configuration substratum, pass into high-temperature steam by the second high-temperature steam pipeline 8 in wall 6, or pass into water coolant by cooling water pipeline 9 in wall 6, and to reach the purpose of regulating substratum preparing tank 1 interior substratum temperature, the temperature condition while preparing to meet substratum.
In the present invention, be provided with stirring gas in the bottom of substratum preparing tank 1 and be blown into a mouthful (not shown), in the process of configuration substratum, be blown into mouth by stirring gas and be blown into pressurized air (nitrogen), when pressurized air (nitrogen) passes through from bottom to top in tank body, can play the effect of stirring preparation, this alr mode has been simplified the mechanical stirring parts in the existing installation, and has avoided pollution and the mixing component drawback not easy to clean brought due to the mechanical stirring parts.
In order to reach the antipollution purpose of requirements at the higher level, in the present invention, adopt pressurized gas up-to-standard after Sterile Filtration to make substratum discharge tank body as power.The top connection of substratum preparing tank 1 is provided with the empty reducing valve 15 of the pressure that the pressurized gas of discharge opeing power liquid-transport pipe-line 14 is provided and controls the tank internal pressure, also is provided with corresponding pneumavalve on pressurized gas liquid-transport pipe-line 14.The top of substratum preparing tank 1 also is provided with dosing hole 16 and tensimeter 17, the preparation initial stage, add commercially available solid medium by dosing hole 16 in tank body, after preparation completes, add pressurized gas by pressurized gas liquid-transport pipe-line 14 in tank body, to increase the air pressure in tank body, tensimeter 17 can be monitored the force value in tank body, after pressure in tank body reaches set(ting)value, can utilize the pressure of pressurized gas to make liquid nutrient medium discharge tank body as power.
The upper end of liquid-transport pipe-line 3 is connected with the bottom of substratum preparing tank 1, and the lower end of liquid-transport pipe-line 3 is connected with filling dividing cell 2, and the provided upstream of liquid-transport pipe-line 3 is equipped with preparing tank pneumavalve 18, and middle part is provided with strainer 19, and downstream is provided with filling pneumavalve 20.Be provided with several filling isocons 21 on filling dividing cell 2, be provided with the time delay switch 22 that adopts PLC to control on each filling isocon 21, time delay switch 22 is controlled the switching time of filling pneumavalve 20 to reach the accurate control of loading amount.
The present invention has simplified the preparation of existing substratum, a plurality of mechanical parts in filling apparatus, as agitator, liquid absorbing pump, filling pump etc.Agitator when stirring raw material, liquid absorbing pump while from tank body, extracting substratum, filling pump be in bottle 23 during filling substratum, all can produce corresponding pollution, these equipment have all increased the contaminated probability of substratum, saved the maintenance that these parts have not only been saved cost, simplified the structure, facilitated equipment in the present invention, the more important thing is the potential pollution source of having stopped in preparation and pouring process, thereby provide guarantee for the safety in production of substratum.
Claims (1)
1. the sterile preparation of a substratum, filling apparatus, it is characterized in that, this equipment comprises the filling dividing cell of substratum preparing tank and precise control loading amount, between substratum preparing tank and filling dividing cell, by liquid-transport pipe-line, be communicated with, the top connection of substratum preparing tank is provided with the empty reducing valve of the pressure that the pressurized gas of discharge opeing power liquid-transport pipe-line is provided and controls the tank internal pressure.
2, the sterile preparation of substratum as claimed in claim 1, filling apparatus, it is characterized in that, the top of described substratum preparing tank also is provided with the second liquid-transport pipe-line, has been arranged in parallel the first high-temperature steam pipeline and vacuum-pumping pipeline and corresponding control valve thereof on this second liquid-transport pipe-line.
3, the sterile preparation of substratum as claimed in claim 2, filling apparatus, is characterized in that, the bottom of described substratum preparing tank is provided with stirring gas and is blown into mouth.
4, the sterile preparation of substratum as claimed in claim 3, filling apparatus, it is characterized in that, described substratum preparing tank comprises interior pot and outer tank body, be provided with airtight wall fully between interior pot and outer tank body, be provided with the 3rd liquid-transport pipe-line be connected with wall on outer tank body, be arranged in parallel the second high-temperature steam pipeline and cooling water pipeline and corresponding control valve thereof on the 3rd liquid-transport pipe-line.
5, the sterile preparation of substratum as claimed in claim 4, filling apparatus, is characterized in that, the bottom of described outer tank body is provided with water shoot and water discharge valve thereof.
6, the sterile preparation of substratum as claimed in claim 2, filling apparatus, is characterized in that, the provided upstream of described liquid-transport pipe-line is equipped with the preparing tank pneumavalve, and middle part is provided with strainer, and downstream is provided with filling pneumavalve.
7, the sterile preparation of substratum as claimed in claim 6, filling apparatus, it is characterized in that, be provided with several filling isocons on described filling dividing cell, be provided with the time delay switch that adopts PLC to control on each filling isocon, this time delay switch is controlled the switching time of described filling pneumavalve to reach the accurate control of loading amount.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110435063 CN102559497B (en) | 2011-12-22 | 2011-12-22 | Sterile preparation, filling method of culture medium and equipment |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110435063 CN102559497B (en) | 2011-12-22 | 2011-12-22 | Sterile preparation, filling method of culture medium and equipment |
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| CN102559497A CN102559497A (en) | 2012-07-11 |
| CN102559497B true CN102559497B (en) | 2013-12-25 |
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| CN 201110435063 Active CN102559497B (en) | 2011-12-22 | 2011-12-22 | Sterile preparation, filling method of culture medium and equipment |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103551062A (en) * | 2013-10-30 | 2014-02-05 | 福州大北农生物技术有限公司 | Equipment for improving preparation efficiency of nutrient solution as well as production method of nutrient solution |
| CN103766205B (en) * | 2014-01-28 | 2016-02-03 | 福建兰水金莲生物科技有限公司 | The bottled medium preparation method of roxburgh anoectochilus terminal bud and packaging device thereof |
| CN104328039B (en) * | 2014-10-30 | 2016-08-03 | 四川科伦药业股份有限公司 | Antibacterial, fungi culture medium compounding system |
| CN107324261A (en) * | 2017-07-28 | 2017-11-07 | 金彩琴 | Industrial chemicals filling apparatus |
| CN108689363A (en) * | 2018-04-28 | 2018-10-23 | 大连普瑞康生物技术有限公司 | A kind of culture medium filling apparatus |
| CN114214244A (en) * | 2021-12-27 | 2022-03-22 | 北京天诚众合科技发展有限公司 | Preparation method, device and application of low-toxicity and low-residue biological algicide |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2126815Y (en) * | 1992-06-24 | 1993-02-10 | 浙江大学 | Aeration mixing device |
| CN2717516Y (en) * | 2004-08-03 | 2005-08-17 | 上海市政工程设计研究院 | Sprial-flow type gas stirring device |
| CN1738763A (en) * | 2002-12-12 | 2006-02-22 | 三得利株式会社 | Agitation member for filler tank, filler tank, and method of agitating liquid in the filler tank |
| CN1850963A (en) * | 2006-03-10 | 2006-10-25 | 张雷 | Microbial culture system and microbial culture method using same |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202379978U (en) * | 2011-12-22 | 2012-08-15 | 四川科伦药业股份有限公司 | Aseptic preparation and filling equipment for culture medium |
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2011
- 2011-12-22 CN CN 201110435063 patent/CN102559497B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2126815Y (en) * | 1992-06-24 | 1993-02-10 | 浙江大学 | Aeration mixing device |
| CN1738763A (en) * | 2002-12-12 | 2006-02-22 | 三得利株式会社 | Agitation member for filler tank, filler tank, and method of agitating liquid in the filler tank |
| CN2717516Y (en) * | 2004-08-03 | 2005-08-17 | 上海市政工程设计研究院 | Sprial-flow type gas stirring device |
| CN1850963A (en) * | 2006-03-10 | 2006-10-25 | 张雷 | Microbial culture system and microbial culture method using same |
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