CN103766205B - The bottled medium preparation method of roxburgh anoectochilus terminal bud and packaging device thereof - Google Patents
The bottled medium preparation method of roxburgh anoectochilus terminal bud and packaging device thereof Download PDFInfo
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- CN103766205B CN103766205B CN201410041570.5A CN201410041570A CN103766205B CN 103766205 B CN103766205 B CN 103766205B CN 201410041570 A CN201410041570 A CN 201410041570A CN 103766205 B CN103766205 B CN 103766205B
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- culture fluid
- terminal bud
- roxburgh anoectochilus
- anoectochilus terminal
- storage tank
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Abstract
The invention provides the bottled medium preparation method of roxburgh anoectochilus terminal bud and packaging device thereof, comprise following preparation method: ripe banana is removed the peel and adds white granulated sugar and Coconut Juice at room temperature stirs pulping; Add agar powder and water, form culture fluid; Prepare sterile culture flask, be filled in blake bottle through culture fluid; Blake bottle is sealed and reserves a small ventilating holes; Degerming 20 ~ the 30min of high temperature in high temperature bactericidal device.The culture fluid that the present invention adopts utilizes existing pulp nutrition and sugar completely, do not adopt any hormone adding material, the present invention is utilized to have also been devised the bottled medium preparing device of roxburgh anoectochilus terminal bud, this apparatus structure is ingenious, can meet the operations such as existing stirring, mixed diluting, filling, gland, simple operation, filling amount is stablized, and efficiency is high, effectively Production requirement can be met.
Description
Technical field
The present invention relates to a kind of bottled medium preparation method of roxburgh anoectochilus terminal bud and packaging device thereof.
Background technology
The culture fluid of roxburgh anoectochilus terminal bud is produced most important for roxburgh anoectochilus terminal bud, stir for culture fluid being filled to the general manual type that adopts in blake bottle at present, the operations such as mixing, filling, gland, so not only time-consuming, effort, and filling amount is also easily by the impact of staff, filling efficiency is low, cannot meet and produce efficient demand.Therefore, are objects that the present invention studies for the problems referred to above.
Summary of the invention
The present invention improves the problems referred to above, and namely the technical problem to be solved in the present invention is that the intellectuality of the existing roxburgh anoectochilus terminal bud mode of production is lower, causes efficiency lower and time-consuming, effort.
First specific embodiments of the present invention is: a kind of bottled medium preparation method of roxburgh anoectochilus terminal bud, is characterized in that, comprise following preparation method:
(1) ripe banana removed the peel and add white granulated sugar and Coconut Juice at room temperature stirs pulping;
(2) add agar powder and water, form culture fluid;
(3) prepare sterile culture flask, be filled in blake bottle through culture fluid;
(4) blake bottle sealed and reserve a small ventilating holes;
(5) the degerming 20 ~ 30min of high temperature in high temperature bactericidal device.
Further, the bottled medium preparation method of a kind of roxburgh anoectochilus terminal bud according to claim 1, each component percentages of described medium is:
Banana 10 ~ 12%;
Coconut Juice 2 ~ 5%;
White granulated sugar 2 ~ 5%;
Agar powder 1 ~ 1.5%;
Surplus is water.
Second specific embodiments of the present invention is: the bottled medium preparing device of a kind of roxburgh anoectochilus terminal bud, it is characterized in that, comprise for slurry agitating device, mixed diluting device, liquid filling device, capping apparatus and high temperature treating device, described slurry agitating device comprises an agitator, the first paddle is provided with in described agitator, mixed diluting device is connected by a slurries suction tube with slurry agitating device, described mixed diluting device comprises a mixture charging basket, water inlet pipe is provided with above described material barrel, the second paddle is provided with in described material barrel, the solution suction tube be connected with liquid filling device is provided with in the mixture charging basket of described mixed diluting device.
Further, described liquid filling device comprises culture fluid temporary storage tank, the bottom of described culture fluid temporary storage tank is provided with one can the traveling table of oscilaltion, described traveling table is connected with some longitudinal ducts in order to filling culture fluid, the upper end of described longitudinal duct extends in culture fluid temporary storage tank, and described longitudinal duct is positioned on culture fluid temporary storage tank outside and is respectively equipped with a sealing ring.
Further, described liquid filling device culture fluid temporary storage tank is connected with overflow pipe, and described finder outlet end stretches in mixture charging basket.
Further, described capping apparatus comprises pressing plate moving up and down, and described pressing plate two ends are provided with the drive unit driving pressing plate to move up and down.
Further, described high temperature treating device comprises one with the closed warehouse of door, and described warehouse is connected with steam access tube and compressor.
Further, temperature sensor and pressure sensor is provided with outside described warehouse.
Compared with prior art, the present invention has following beneficial effect: the culture fluid that the present invention adopts utilizes existing pulp nutrition and sugar completely, do not adopt any hormone adding material, utilize the present invention to have also been devised the bottled medium preparing device of roxburgh anoectochilus terminal bud, this apparatus structure is ingenious, can meet the operations such as existing stirring, mixed diluting, filling, gland, simple operation, filling amount is stablized, and efficiency is high, effectively can meet Production requirement.
Accompanying drawing explanation
Fig. 1 is roxburgh anoectochilus terminal bud of the present invention bottled medium preparing device overall structure schematic diagram.
Fig. 2 is high temperature treating device structural representation of the present invention.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described in detail.
A kind of bottled medium preparation method of roxburgh anoectochilus terminal bud, is characterized in that, comprise following preparation method:
(1) ripe banana removed the peel and add white granulated sugar and Coconut Juice at room temperature stirs pulping;
(2) add agar powder and water, form culture fluid;
(3) prepare sterile culture flask, be filled in blake bottle through culture fluid;
(4) blake bottle sealed and reserve a small ventilating holes;
(5) the degerming 20 ~ 30min of high temperature in high temperature bactericidal device.
In the present embodiment, each constituent mass percentage of medium is: banana 12%, Coconut Juice 2.5%, white granulated sugar 5%, agar powder 1%, and surplus is water.
If Fig. 1 ~ 2 are the present embodiment schematic diagram, the bottled medium preparing device of a kind of roxburgh anoectochilus terminal bud, comprises for slurry agitating device, mixed diluting device, liquid filling device, capping apparatus and high temperature treating device;
Slurry agitating device: for ripe banana is broken into banana puree, add Coconut Juice and white sugar while stirring;
Mixed diluting device: filling to prepare for slurry being carried out post processing dilution;
Slurry agitating device comprises an agitator 10, is provided with the first paddle 110 in described agitator 10;
Mixed diluting device comprises a mixture charging basket 20, is provided with water inlet pipe 210 above described material barrel 20, is provided with the second paddle 220 in described material barrel.
Mixed diluting device is connected by a slurries suction tube 230 with slurry agitating device, and the raw material mixed slurries such as banana puree Coconut Juice are pumped in mixture charging basket 20 by suction pump by slurries suction tube 230.
The solution suction tube 240 be connected with liquid filling device is provided with, for being pumped in filling apparatus by culture fluid in the mixture charging basket of mixed diluting device.
Liquid filling device comprises culture fluid temporary storage tank 30, the bottom of described culture fluid temporary storage tank 30 is provided with one can the traveling table 310 of oscilaltion, described traveling table is connected with some longitudinal ducts 320 in order to filling culture fluid, the upper end of described longitudinal duct 320 extends in culture fluid temporary storage tank 30, and described longitudinal duct is positioned on culture fluid temporary storage tank outside and is respectively equipped with a sealing ring.
Liquid filling device culture fluid temporary storage tank is connected with overflow pipe 330, and described overflow pipe 330 port of export gos deep in mixture charging basket.
Effect due to solution suction tube 240 overflow pipe 330 makes culture fluid remain on fixing liquid level, like this when needs are filling, the traveling table 310 being controlled oscilaltion by both sides cylinder when setting the walking of stroke after, can quantitative filling be realized.
Get out the relevant blake bottle 100 cleaned afterwards, put into framework dish, alignment is put into immediately below filling apparatus conduit 320, opens liquid filling machine, and culture fluid is flowed into each blake bottle 100 in culture fluid temporary storage tank 30 by conduit 320, completes filling.In the present invention, each pipeline is equipped with control valve.
Filling complete after by manually placing blake bottle lid on bottle cap, move into below capping apparatus.
Capping apparatus comprises pressing plate 40 moving up and down, described pressing plate two ends are provided with the drive unit driving pressing plate to move up and down, drive unit can be hydraulic cylinder or cylinder, after pressing plate presses down, the capping being positioned over blake bottle 100 is fastened on blake bottle bottleneck, realizes encapsulation.
High temperature treating device:
Need to carry out virus killing after capping degerming, high temperature treating device comprises one with the closed warehouse of door, described warehouse is connected with steam access tube and compressor, temperature sensor and pressure sensor is provided with outside warehouse, temperature sensor is connected with the control system be arranged at outside warehouse with pressure sensor, shows concrete pressure and temperature parameter by the Electronic Display Panel of warehouse simultaneously.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (3)
1. the bottled medium preparing device of roxburgh anoectochilus terminal bud, it is characterized in that, comprise for slurry agitating device, mixed diluting device, liquid filling device, capping apparatus and high temperature treating device, described slurry agitating device comprises an agitator, the first paddle is provided with in described agitator, mixed diluting device is connected by a slurries suction tube with slurry agitating device, described mixed diluting device comprises a mixture charging basket, water inlet pipe is provided with above described material barrel, the second paddle is provided with in described material barrel, the solution suction tube be connected with liquid filling device is provided with in the mixture charging basket of described mixed diluting device, described capping apparatus comprises pressing plate moving up and down, described pressing plate two ends are provided with the drive unit driving pressing plate to move up and down, described high temperature treating device comprises one with the closed warehouse of door, described warehouse is connected with steam access tube and compressor, temperature sensor and pressure sensor is provided with outside described warehouse.
2. the bottled medium preparing device of a kind of roxburgh anoectochilus terminal bud according to claim 1, described liquid filling device comprises culture fluid temporary storage tank, the bottom of described culture fluid temporary storage tank is provided with one can the traveling table of oscilaltion, described traveling table is connected with some longitudinal ducts in order to filling culture fluid, the upper end of described longitudinal duct extends in culture fluid temporary storage tank, and described longitudinal duct is positioned on culture fluid temporary storage tank outside and is respectively equipped with a sealing ring.
3. the bottled medium preparing device of a kind of roxburgh anoectochilus terminal bud according to claim 2, described liquid filling device culture fluid temporary storage tank is connected with overflow pipe, and described finder outlet end stretches in mixture charging basket.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410041570.5A CN103766205B (en) | 2014-01-28 | 2014-01-28 | The bottled medium preparation method of roxburgh anoectochilus terminal bud and packaging device thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410041570.5A CN103766205B (en) | 2014-01-28 | 2014-01-28 | The bottled medium preparation method of roxburgh anoectochilus terminal bud and packaging device thereof |
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| CN103766205A CN103766205A (en) | 2014-05-07 |
| CN103766205B true CN103766205B (en) | 2016-02-03 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104401916A (en) * | 2014-11-24 | 2015-03-11 | 重庆市川瑞俞厨食品有限公司 | Juice filling machine |
| CN104609354B (en) * | 2015-01-28 | 2017-08-01 | 山东鑫科生物科技股份有限公司 | Filling machine |
| CN104756668B (en) * | 2015-03-23 | 2016-08-24 | 江苏华绿生物科技股份有限公司 | A kind of Hypsizygus marmoreus harvester and collecting method thereof |
| CN108308038A (en) * | 2018-05-09 | 2018-07-24 | 美丽国土（北京）生态环境工程技术研究院有限公司 | The packaging device and plant tissue culture system of culture medium |
| CN110301296A (en) * | 2019-08-06 | 2019-10-08 | 山东美奥生物工程有限公司 | A kind of cordyceps sinensis culture charger |
| CN112352676A (en) * | 2020-10-28 | 2021-02-12 | 广西壮族自治区农业科学院 | Multipoint automatic quantitative tissue culture solution stirring and filling machine |
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| CN87204189U (en) * | 1987-05-04 | 1988-06-29 | 湖南省农业机械研究所 | Small liquid metering lid-pressing machine |
| CN102774800A (en) * | 2012-07-17 | 2012-11-14 | 台州乾元生物科技有限公司 | Culture medium cold-filling preparation process |
| CN203187384U (en) * | 2013-02-27 | 2013-09-11 | 福建红宝山生物科技有限公司 | Quantitative anoectochilus roxburghii culture liquid filling device |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN2425854Y (en) * | 2000-05-07 | 2001-04-04 | 衡阳市千山制药机械有限公司 | Novel filling machine |
| JP5292609B2 (en) * | 2008-08-07 | 2013-09-18 | 東洋製罐株式会社 | Method for verifying "sterility level of container" in aseptic filling system and aseptic filling system |
| CN201442856U (en) * | 2008-11-20 | 2010-04-28 | 孙学义 | Full-electronic electric aseptic filling machine |
| EP2537794A1 (en) * | 2011-06-22 | 2012-12-26 | Skånemejerier AB | New filling method |
| CN102559497B (en) * | 2011-12-22 | 2013-12-25 | 四川科伦药业股份有限公司 | Sterile preparation, filling method of culture medium and equipment |
| CN202492352U (en) * | 2012-03-07 | 2012-10-17 | 中国热带农业科学院热带作物品种资源研究所 | High-efficient and quantitative adjustable culture medium sub-packaging device |
| CN103466527A (en) * | 2013-03-27 | 2013-12-25 | 上海辉敦包装科技有限公司 | Fully-automatic packaging system of disposable plating medium |
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2014
- 2014-01-28 CN CN201410041570.5A patent/CN103766205B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87204189U (en) * | 1987-05-04 | 1988-06-29 | 湖南省农业机械研究所 | Small liquid metering lid-pressing machine |
| CN102774800A (en) * | 2012-07-17 | 2012-11-14 | 台州乾元生物科技有限公司 | Culture medium cold-filling preparation process |
| CN203187384U (en) * | 2013-02-27 | 2013-09-11 | 福建红宝山生物科技有限公司 | Quantitative anoectochilus roxburghii culture liquid filling device |
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| CN103766205A (en) | 2014-05-07 |
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