CN103766205A - Preparation method for anoectochilus roxburghii bottled culture medium and subpackaging device thereof - Google Patents
Preparation method for anoectochilus roxburghii bottled culture medium and subpackaging device thereof Download PDFInfo
- Publication number
- CN103766205A CN103766205A CN201410041570.5A CN201410041570A CN103766205A CN 103766205 A CN103766205 A CN 103766205A CN 201410041570 A CN201410041570 A CN 201410041570A CN 103766205 A CN103766205 A CN 103766205A
- Authority
- CN
- China
- Prior art keywords
- culture fluid
- terminal bud
- bottled
- roxburgh anoectochilus
- anoectochilus terminal
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- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- 241000934230 Anoectochilus roxburghii Species 0.000 title abstract 3
- 239000001963 growth medium Substances 0.000 title abstract 3
- 238000011177 media preparation Methods 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 17
- 239000000203 mixture Substances 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 235000020415 coconut juice Nutrition 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims abstract description 8
- 238000003756 stirring Methods 0.000 claims abstract description 8
- 229920001817 Agar Polymers 0.000 claims abstract description 7
- 239000008272 agar Substances 0.000 claims abstract description 7
- 235000021552 granulated sugar Nutrition 0.000 claims abstract description 7
- 239000000843 powder Substances 0.000 claims abstract description 7
- 238000007789 sealing Methods 0.000 claims abstract description 7
- 239000012531 culture fluid Substances 0.000 claims description 31
- 241000719837 Anoectochilus Species 0.000 claims description 20
- 239000002002 slurry Substances 0.000 claims description 15
- 238000007865 diluting Methods 0.000 claims description 14
- 238000003825 pressing Methods 0.000 claims description 10
- 241000234295 Musa Species 0.000 claims description 9
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims description 9
- 230000000844 anti-bacterial effect Effects 0.000 claims description 3
- 238000004537 pulping Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000012895 dilution Substances 0.000 abstract description 2
- 238000010790 dilution Methods 0.000 abstract description 2
- 229940088597 hormone Drugs 0.000 abstract description 2
- 239000005556 hormone Substances 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 235000000346 sugar Nutrition 0.000 abstract description 2
- 230000001954 sterilising effect Effects 0.000 abstract 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract 2
- 240000008790 Musa x paradisiaca Species 0.000 abstract 1
- 235000021015 bananas Nutrition 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 210000004907 gland Anatomy 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000011268 mixed slurry Substances 0.000 description 1
- 238000012805 post-processing Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
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- Y02P60/216—
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a preparation method for an anoectochilus roxburghii bottled culture medium and a subpackaging device thereof. The preparation method comprises the following steps of peeling off ripe bananas, adding white granulated sugar and coconut juice, and stirring the mixture into pulp under room temperature; adding agar powder and water to form a culture liquid; preparing a germfree culture bottle, and filling the culture bottle with the culture liquid; sealing the culture bottle, and reserving a small ventilating hole; performing high-temperature sterilization on the culture bottle in a high-temperature sterilization device for 20-30min. The culture liquid adopted by the preparation method makes full use of the nutrition and the sugar of the conventional fruit pulps, and any hormone adding material is not adopted. The invention also discloses an anoectochilus roxburghii bottled culture medium preparation device. The device is ingenious in structure, can conform to the conventional working procedures such as stirring, mixed dilution, filling and covering, is convenient to operate, stable in filling amount and high in efficiency and can effectively meet the production requirement.
Description
Technical field
The present invention relates to the bottled medium preparation method of a kind of roxburgh anoectochilus terminal bud and packaging device thereof.
Background technology
the culture fluid of roxburgh anoectochilus terminal bud is produced most important for roxburgh anoectochilus terminal bud, stir for culture fluid being filled to the general manual type that adopts in blake bottle at present, mix, the operation such as filling, gland, so not only time-consuming, effort, and filling amount is also easily subject to staff's impact, filling efficiency is low, cannot meet the efficient demand of producing.Therefore are, objects that the present invention studies for the problems referred to above.
Summary of the invention
The present invention improves the problems referred to above, and the technical problem to be solved in the present invention is that the intellectuality of the existing roxburgh anoectochilus terminal bud mode of production is lower, cause efficiency lower and time-consuming, effort.
The first specific embodiments of the present invention is: the bottled medium preparation method of a kind of roxburgh anoectochilus terminal bud, it is characterized in that, and comprise following preparation method:
(1) ripe banana removed the peel and added white granulated sugar and Coconut Juice at room temperature to stir pulping;
(2) add agar powder and water, form culture fluid;
(3) prepare sterile culture flask, be filled in blake bottle through culture fluid;
(4) blake bottle is sealed and is reserved a small ventilating holes;
(5) high temperature degerming 20～30min in high temperature bactericidal device.
Further, the bottled medium preparation method of a kind of roxburgh anoectochilus terminal bud according to claim 1, the each component percentages of described medium is:
Banana 10～12%;
Coconut Juice 2～5%;
White granulated sugar 2～5%;
Agar powder 1～1.5%;
Surplus is water.
The second specific embodiments of the present invention is: the bottled medium preparation facilities of a kind of roxburgh anoectochilus terminal bud, it is characterized in that, comprise for slurry agitating device, mixed diluting device, liquid filling device, capping apparatus and high temperature treating device, described slurry agitating device comprises an agitator, in described agitator, be provided with the first paddle, mixed diluting device is connected by a slurries suction tube with slurry agitating device, described mixed diluting device comprises a mixture charging basket, described material barrel top is provided with water inlet pipe, in described material barrel, be provided with the second paddle, in the mixture charging basket of described mixed diluting device, be provided with the solution suction tube being connected with liquid filling device.
Further, described liquid filling device comprises that culture fluid keeps in tank, the bottom of the temporary tank of described culture fluid be provided with one can oscilaltion traveling table, on described traveling table, be connected with some longitudinal ducts in order to filling culture fluid, the upper end of described longitudinal duct extends in the temporary tank of culture fluid, and described longitudinal duct is positioned on the temporary tank of culture fluid outside and is respectively equipped with a sealing ring.
Further, the temporary tank of described liquid filling device culture fluid is connected with overflow pipe, and the described overflow pipe port of export stretches in mixture charging basket.
Further, described capping apparatus comprises pressing plate moving up and down, and described pressing plate two ends are provided with the drive unit that drives pressing plate to move up and down.
Further, described high temperature treating device comprises a sealing warehouse with door, and described warehouse is connected with steam access tube and compressor.
Further, described warehouse is provided with temperature sensor and pressure sensor outward.
Compared with prior art, the present invention has following beneficial effect: the culture fluid that the present invention adopts utilizes existing pulp nutrition and sugar completely, do not adopt any hormone to add material, utilize the present invention also to design the bottled medium preparation facilities of roxburgh anoectochilus terminal bud, this apparatus structure is ingenious, can meet the operations such as existing stirring, mixed diluting, filling, gland, simple operation, filling amount is stable, and efficiency is high, can effectively meet Production requirement.
Accompanying drawing explanation
Fig. 1 is the bottled medium preparation facilities of roxburgh anoectochilus terminal bud of the present invention overall structure schematic diagram.
Fig. 2 is high temperature treating device structural representation of the present invention.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described in detail.
The bottled medium preparation method of a kind of roxburgh anoectochilus terminal bud, is characterized in that, comprises following preparation method:
(1) ripe banana removed the peel and added white granulated sugar and Coconut Juice at room temperature to stir pulping;
(2) add agar powder and water, form culture fluid;
(3) prepare sterile culture flask, be filled in blake bottle through culture fluid;
(4) blake bottle is sealed and is reserved a small ventilating holes;
(5) high temperature degerming 20～30min in high temperature bactericidal device.
In the present embodiment, the each constituent mass percentage of medium is: banana 12%, Coconut Juice 2.5%, white granulated sugar 5%, agar powder 1%, surplus is water.
If Fig. 1～2 are the present embodiment schematic diagram, the bottled medium preparation facilities of a kind of roxburgh anoectochilus terminal bud, comprises for slurry agitating device, mixed diluting device, liquid filling device, capping apparatus and high temperature treating device;
Slurry agitating device: for ripe banana is broken into banana puree, add Coconut Juice and white sugar when stirring;
Mixed diluting device: filling to prepare for slurry is carried out to post processing dilution;
Slurry agitating device comprises an agitator 10, is provided with the first paddle 110 in described agitator 10;
Mixed diluting device comprises a mixture charging basket 20, and described material barrel 20 tops are provided with water inlet pipe 210, are provided with the second paddle 220 in described material barrel.
Mixed diluting device is connected by a slurries suction tube 230 with slurry agitating device, and slurries suction tube 230 is pumped to the raw material mixed slurries such as banana puree Coconut Juice in mixture charging basket 20 by suction pump.
In the mixture charging basket of mixed diluting device, be provided with the solution suction tube 240 being connected with liquid filling device, for culture fluid is pumped in filling apparatus.
Liquid filling device comprises that culture fluid keeps in tank 30, the bottom of the temporary tank 30 of described culture fluid be provided with one can oscilaltion traveling table 310, on described traveling table, be connected with some longitudinal ducts 320 in order to filling culture fluid, the upper end of described longitudinal duct 320 extends in the temporary tank 310 of culture fluid, and described longitudinal duct is positioned on the temporary tank of culture fluid outside and is respectively equipped with a sealing ring.
The temporary tank of liquid filling device culture fluid is connected with overflow pipe 330, and described overflow pipe 330 ports of export go deep in mixture charging basket.
Because the effect of solution suction tube 240 overflow pipes 330 is to make culture fluid remain on fixing liquid level, like this in the time that needs are filling, after the traveling table 310 of both sides cylinder control oscilaltion is in the time setting the walking of stroke, can realize quantitative filling.
Be ready to afterwards relevant clean blake bottle 100, put into framework dish, alignment put into filling apparatus conduit 320 under, open liquid filling machine, culture fluid from temporary each blake bottle 100 of the interior inflow of tank 30 of culture fluid, completes filling by conduit 320.In the present invention, each pipeline is equipped with control valve.
After filling completing, by manually place blake bottle lid on bottle cap, move into capping apparatus below.
Capping apparatus comprises pressing plate 40 moving up and down, described pressing plate two ends are provided with the drive unit that drives pressing plate to move up and down, drive unit can be hydraulic cylinder or cylinder, after pressing plate presses down, the capping that is positioned over blake bottle 100 is fastened on to blake bottle bottleneck, realizes encapsulation.
High temperature treating device:
After capping, need the degerming of killing virus, high temperature treating device comprises a sealing warehouse with door, described warehouse is connected with steam access tube and compressor, warehouse is provided with temperature sensor and pressure sensor outward, temperature sensor is connected with the control system that is arranged at warehouse outside with pressure sensor, shows concrete pressure and temperature parameter by the Electronic Display Panel of warehouse simultaneously.
The foregoing is only preferred embodiment of the present invention, all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (8)
1. the bottled medium preparation method of roxburgh anoectochilus terminal bud, is characterized in that, comprises following preparation method:
(1) ripe banana removed the peel and added white granulated sugar and Coconut Juice at room temperature to stir pulping;
(2) add agar powder and water, form culture fluid;
(3) prepare sterile culture flask, be filled in blake bottle through culture fluid;
(4) blake bottle is sealed and is reserved a small ventilating holes;
(5) high temperature degerming 20～30min in high temperature bactericidal device.
2. the bottled medium preparation method of a kind of roxburgh anoectochilus terminal bud according to claim 1, the each component percentages of described medium is:
Banana 10～12%;
Coconut Juice 2～5%;
White granulated sugar 2～5%;
Agar powder 1～1.5%;
Surplus is water.
3. the bottled medium preparation facilities of roxburgh anoectochilus terminal bud, it is characterized in that, comprise for slurry agitating device, mixed diluting device, liquid filling device, capping apparatus and high temperature treating device, described slurry agitating device comprises an agitator, in described agitator, be provided with the first paddle, mixed diluting device is connected by a slurries suction tube with slurry agitating device, described mixed diluting device comprises a mixture charging basket, described material barrel top is provided with water inlet pipe, in described material barrel, be provided with the second paddle, in the mixture charging basket of described mixed diluting device, be provided with the solution suction tube being connected with liquid filling device.
4. the bottled medium preparation facilities of a kind of roxburgh anoectochilus terminal bud according to claim 3, described liquid filling device comprises that culture fluid keeps in tank, the bottom of the temporary tank of described culture fluid be provided with one can oscilaltion traveling table, on described traveling table, be connected with some longitudinal ducts in order to filling culture fluid, the upper end of described longitudinal duct extends in the temporary tank of culture fluid, and described longitudinal duct is positioned on the temporary tank of culture fluid outside and is respectively equipped with a sealing ring.
5. the bottled medium preparation facilities of a kind of roxburgh anoectochilus terminal bud according to claim 4, the temporary tank of described liquid filling device culture fluid is connected with overflow pipe, and the described overflow pipe port of export stretches in mixture charging basket.
6. the bottled medium preparation facilities of a kind of roxburgh anoectochilus terminal bud according to claim 3, described capping apparatus comprises pressing plate moving up and down, described pressing plate two ends are provided with the drive unit that drives pressing plate to move up and down.
7. the bottled medium preparation facilities of a kind of roxburgh anoectochilus terminal bud according to claim 3, described high temperature treating device comprises a sealing warehouse with door, described warehouse is connected with steam access tube and compressor.
8. the bottled medium preparation facilities of a kind of roxburgh anoectochilus terminal bud according to claim 7, described warehouse is provided with temperature sensor and pressure sensor outward.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410041570.5A CN103766205B (en) | 2014-01-28 | 2014-01-28 | The bottled medium preparation method of roxburgh anoectochilus terminal bud and packaging device thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410041570.5A CN103766205B (en) | 2014-01-28 | 2014-01-28 | The bottled medium preparation method of roxburgh anoectochilus terminal bud and packaging device thereof |
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| Publication Number | Publication Date |
|---|---|
| CN103766205A true CN103766205A (en) | 2014-05-07 |
| CN103766205B CN103766205B (en) | 2016-02-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410041570.5A Expired - Fee Related CN103766205B (en) | 2014-01-28 | 2014-01-28 | The bottled medium preparation method of roxburgh anoectochilus terminal bud and packaging device thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104401916A (en) * | 2014-11-24 | 2015-03-11 | 重庆市川瑞俞厨食品有限公司 | Juice filling machine |
| CN104609354A (en) * | 2015-01-28 | 2015-05-13 | 山东鑫科生物科技股份有限公司 | Filling machine |
| CN104756668A (en) * | 2015-03-23 | 2015-07-08 | 江苏华绿生物科技股份有限公司 | Hypsizigus marmoreus harvester and harvesting method thereof |
| CN108308038A (en) * | 2018-05-09 | 2018-07-24 | 美丽国土（北京）生态环境工程技术研究院有限公司 | The packaging device and plant tissue culture system of culture medium |
| CN110301296A (en) * | 2019-08-06 | 2019-10-08 | 山东美奥生物工程有限公司 | A kind of cordyceps sinensis culture charger |
| CN112352676A (en) * | 2020-10-28 | 2021-02-12 | 广西壮族自治区农业科学院 | Multipoint automatic quantitative tissue culture solution stirring and filling machine |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104401916A (en) * | 2014-11-24 | 2015-03-11 | 重庆市川瑞俞厨食品有限公司 | Juice filling machine |
| CN104609354A (en) * | 2015-01-28 | 2015-05-13 | 山东鑫科生物科技股份有限公司 | Filling machine |
| CN104756668A (en) * | 2015-03-23 | 2015-07-08 | 江苏华绿生物科技股份有限公司 | Hypsizigus marmoreus harvester and harvesting method thereof |
| CN108308038A (en) * | 2018-05-09 | 2018-07-24 | 美丽国土（北京）生态环境工程技术研究院有限公司 | The packaging device and plant tissue culture system of culture medium |
| CN110301296A (en) * | 2019-08-06 | 2019-10-08 | 山东美奥生物工程有限公司 | A kind of cordyceps sinensis culture charger |
| CN112352676A (en) * | 2020-10-28 | 2021-02-12 | 广西壮族自治区农业科学院 | Multipoint automatic quantitative tissue culture solution stirring and filling machine |
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| Publication number | Publication date |
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| CN103766205B (en) | 2016-02-03 |
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