CN202492352U - A high-efficiency quantitative and adjustable medium distribution equipment - Google Patents

A high-efficiency quantitative and adjustable medium distribution equipment Download PDF

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CN202492352U
CN202492352U CN 201220109170 CN201220109170U CN202492352U CN 202492352 U CN202492352 U CN 202492352U CN 201220109170 CN201220109170 CN 201220109170 CN 201220109170 U CN201220109170 U CN 201220109170U CN 202492352 U CN202492352 U CN 202492352U
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controller
culture medium
peristaltic pump
quantitative
medium
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李志英
徐立
李克烈
马千全
黄碧兰
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Tropical Crops Genetic Resources Institute CATAS
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Tropical Crops Genetic Resources Institute CATAS
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Abstract

The utility model discloses a high-efficiency quantitative adjustable culture medium split charging device, which comprises a culture medium barrel, a peristaltic pump head, a culture medium conveying pipe, a split charging pipe fixer, a quantitative controller and a controller; one end of the culture medium conveying pipe is connected with the culture medium barrel and passes through the peristaltic pump head, and the other end of the culture medium conveying pipe is fixed to the split charging pipe fixing device; one end of the quantitative controller is connected with the peristaltic pump, and the other end of the quantitative controller is connected with one end of the controller; the other end of the controller is connected with the peristaltic pump; the culture medium barrel is internally provided with a liftable automatic rotating stirrer, and the automatic rotating stirrer is fixed on the bracket. The utility model discloses can carry out automation, quantitative partial shipment to a large amount of culture mediums simultaneously, single partial shipment quantity can dispose pump head quantity as required, easy operation, high efficiency, even the low people of culture level also can be fast, skilled grasp.

Description

一种高效定量可调培养基分装设备A high-efficiency quantitative and adjustable medium distribution equipment

技术领域 technical field

本实用新型涉及机械、电动设备技术领域,尤其涉及对粘稠或易沉淀液体培养基的高效、定量分装设备。The utility model relates to the technical field of machinery and electric equipment, in particular to high-efficiency and quantitative packaging equipment for viscous or easy-to-precipitate liquid culture medium.

背景技术 Background technique

传统制备分装培养基到培养皿、试管、培养瓶等培养容器的方法,通常是在定量容器中制备一定量的培养基,混匀后,倒入培养皿等培养容器中。这样的制作方法虽然简单,但如果在工厂化生产中大量配制培养基,就显得繁琐而费时了。为了解决培养基分装繁琐、费时等问题,许多培养基分装器、分装设备相继问世。The traditional method of preparing subpackaged medium to culture containers such as petri dishes, test tubes, and culture bottles is usually to prepare a certain amount of culture medium in a quantitative container, mix it, and pour it into culture containers such as petri dishes. Although such a production method is simple, it would be cumbersome and time-consuming to prepare a large amount of culture medium in factory production. In order to solve the problems of cumbersome and time-consuming media packaging, many media dispensers and packaging equipment have come out one after another.

进口设备如瑞士B IOTOOL公司推出Petriswiss PS900(P)全自动平板分装系统。该系统分装速度快、分装体积准确,及平板分装、培养基均分、平板自动编号、平板匀化、平板自动分装及触屏等多项先进技术于一体,目前正广泛应用于研究机构、高校、化工行业、制药行业、医院、食品及微生物等行业的相关领域。其存在的缺点:由于该系统智能化程度较高,因此操作人员需要较高的文化水平,而文化水平较低的人则无法操作该系统,不适合与普通农户;另外,该系统仅适合对平板进行分装,不适合对培养瓶、培养杯、培养盒、试管等培养容器进行分装,不适合种苗工厂化生产行业。Imported equipment such as the Swiss B IOTOOL company launched the Petriswiss PS900 (P) automatic flat-panel packaging system. The system has fast dispensing speed, accurate dispensing volume, and many advanced technologies such as plate dispensing, culture medium equalization, plate automatic numbering, plate homogenization, plate automatic dispensing and touch screen. It is currently being widely used in Related fields of research institutions, universities, chemical industry, pharmaceutical industry, hospital, food and microbiology and other industries. Its disadvantages: due to the high degree of intelligence of the system, operators need a high level of education, and people with low education levels cannot operate the system, which is not suitable for ordinary farmers; in addition, the system is only suitable for farmers. It is not suitable for subpackaging of culture containers such as culture bottles, culture cups, culture boxes, and test tubes, and is not suitable for the industrialized production of seedlings.

Agarfill是一款简单实用的意大利产的手动培养基制备分装器,该装置适合样品量较少的用户,可整体高压灭菌,最大容积2.5升。该设备的缺点是:容器容积小,不适合于大批量培养基的制备。Agarfill is a simple and practical Italian-made manual media preparation dispenser. This device is suitable for users with small sample volumes. It can be autoclaved as a whole and has a maximum volume of 2.5 liters. The disadvantage of this equipment is: the volume of the container is small, and it is not suitable for the preparation of large batches of medium.

国产分装系统种类多样,主要分为三类:There are various types of domestic sub-packaging systems, which are mainly divided into three categories:

(1)软管输液:将培养容器摆好,靠重力作用将培养基引入容器。一人分装,手捏软管头,根据经验控制流液的时间,然后转入下一瓶。每瓶分装时间2秒以上,分装量不均匀;(1) Tube infusion: place the culture container well, and introduce the culture medium into the container by gravity. One person divides the package, pinches the hose head, controls the time of the liquid flow according to experience, and then transfers to the next bottle. The dispensing time of each bottle is more than 2 seconds, and the dispensing volume is uneven;

(2)自来水管放液:在搅拌桶下最多安装3个水龙头,3人围坐,各拿一筐容器,每次拿1-2瓶,打开水龙头将混匀的培养基放到容器中,根据经验确定放出量,将灌注培养基的容器放入另一筐,再拿空的容器继续分装。在一定时间内由于放液口增加而加快了培养基分装速度。但分装一瓶培养基的时间平均为2秒以上,分装量不均匀,投入人力较多。(2) Drainage from the tap water pipe: Install up to 3 faucets under the mixing bucket, 3 people sit around, each take a basket container, each time take 1-2 bottles, turn on the faucet and put the mixed culture medium into the container, Determine the release amount based on experience, put the container filled with medium into another basket, and then continue to divide the empty container. In a certain period of time, the filling speed of the medium is accelerated due to the increase of the liquid discharge port. But the time of distributing a bottle of culture medium is more than 2 seconds on average, and dispensing quantity is uneven, and input manpower is more.

(3)定量分液:采用一种类似桶装水结构的设备,当用容器顶开出液开关时,并运行一次,定量放出液体。该设备一人操作,定量分装。缺点:一次只能1瓶,分装完1瓶,放到别处,再取空瓶分装,每瓶分装时间1.5秒以上,耗费人力较多。(3) Quantitative liquid separation: A device similar to the structure of bottled water is used. When the container is used to push the liquid outlet switch, and run once, the liquid is released quantitatively. The equipment is operated by one person and quantitatively distributed. Disadvantages: Only one bottle can be used at a time. After filling one bottle, put it elsewhere, and then take the empty bottle and repack it. The filling time of each bottle is more than 1.5 seconds, which consumes more manpower.

(4)蠕动泵分液:现有蠕动泵带动蠕动泵头的分液装置,缺点:仅限于1个蠕动泵头,每次只能分装1瓶(管),效率较低。(4) Liquid dispensing by peristaltic pump: The existing liquid dispensing device driven by the peristaltic pump head has disadvantages: only one peristaltic pump head can only be dispensed into one bottle (tube) each time, and the efficiency is low.

(5)灌装机:多用于白酒、饮料等工业,需要配有传送带等,成本较高。(5) Filling machine: It is mostly used in liquor, beverage and other industries, and needs to be equipped with conveyor belts, etc., and the cost is relatively high.

由于目前这些装置存在的低效、人力密集、分装不准确等缺点,不适合应用于工厂化种苗生产。Due to the shortcomings of low efficiency, labor-intensive, inaccurate packaging and other shortcomings of these devices, they are not suitable for industrial seedling production.

实用新型内容 Utility model content

本实用新型的目的在于提供一种高效定量可调分装培养基的设备,除了自动搅拌,适合粘稠或易沉淀的培养基外,还能够大批量、高效、定量分装培养基,使用简单,文化水平低的人也能快速、数量掌握。The purpose of this utility model is to provide a high-efficiency, quantitative and adjustable subpackage medium equipment. In addition to automatic stirring, which is suitable for viscous or easy-to-settling medium, it can also be used in large batches, high efficiency, quantitative subpackage of medium, and is easy to use. , People with low cultural level can also master it quickly and quantitatively.

为了实现上述目的,本实用新型的技术方案为:提供一种高效定量可调培养基分装设备,其中,包括培养基桶、蠕动泵、蠕动泵头、培养基输送管、分装管固定器、定量控制器和控制器;所述培养基输送管一端连接培养基桶,经过蠕动泵头,另一端固定到分装管固定器;定量控制器一端与蠕动泵连接,另一端与控制器的一端连接;控制器的另一端再与蠕动泵连接;所述培养基桶内设有可升降的自动旋转搅拌器,自动旋转搅拌器固定在支架上。In order to achieve the above object, the technical solution of the present utility model is to provide a high-efficiency quantitative adjustable medium subpackaging equipment, which includes a medium bucket, a peristaltic pump, a peristaltic pump head, a medium delivery tube, and a subpackage tube holder , a quantitative controller and a controller; one end of the medium conveying pipe is connected to the medium barrel, passes through the peristaltic pump head, and the other end is fixed to the sub-packaging tube holder; one end of the quantitative controller is connected with the peristaltic pump, and the other end is connected with the controller One end is connected; the other end of the controller is connected with the peristaltic pump; the culture medium barrel is provided with a liftable automatic rotating agitator, and the automatic rotating agitator is fixed on the support.

本实用新型的有益效果是:本实用新型的培养基桶可实现对培养基的自动搅拌,使粘稠或易沉淀的培养基能充分混匀,无需对含有琼脂或卡拉胶的培养基加热融化;可同时对大量的培养基进行自动、定量分装,单次分装数量可根据需要配置泵头数量,操作简单、高效,即使文化水平低的人也可快速、熟练掌握。The beneficial effects of the utility model are: the culture medium bucket of the utility model can realize the automatic stirring of the culture medium, so that the viscous or easily precipitated culture medium can be fully mixed, and there is no need to heat and melt the culture medium containing agar or carrageenan ;It can automatically and quantitatively dispense a large amount of medium at the same time, and the number of pump heads can be configured according to the needs of a single dispense. The operation is simple and efficient, and even people with low education levels can quickly and skillfully master it.

附图说明 Description of drawings

图1为本实用新型高效定量可调培养基分装设备的结构示意图;Fig. 1 is the structural representation of the utility model efficient quantitative adjustable medium packing equipment;

图中:1、支架;2、自动旋转搅拌器;3、培养基桶;4、培养基输送管;5、蠕动泵头;6、蠕动泵;7、控制器;8、定量控制器;9、分装管固定器。In the figure: 1. Support; 2. Automatic rotating agitator; 3. Medium bucket; 4. Medium delivery pipe; 5. Peristaltic pump head; 6. Peristaltic pump; 7. Controller; 8. Quantitative controller; 9 , Dispensing tube holder.

具体实施方式 Detailed ways

为了详细说明本实用新型高效定量可调培养基分装设备的技术内容、构造特征、以下结合实施方式并配合附图作进一步说明。In order to describe in detail the technical content and structural features of the utility model's high-efficiency and quantitatively adjustable medium dispensing equipment, the following will be further described in conjunction with the embodiments and accompanying drawings.

如图1所示,本实用新型高效定量可调培养基分装设备,包括培养基桶3、蠕动泵6、蠕动泵头5、培养基输送管4、分装管固定器9、定量控制器8和控制器7;所述培养基输送管4一端连接培养基桶3,经过蠕动泵头5,另一端固定到分装管固定器9;定量控制器8一端与蠕动泵6连接,另一端与控制器7连接,控制器7的另一端再与蠕动泵6连接;所述培养基桶3内设有可升降的自动旋转搅拌器2,自动旋转搅拌器2固定在支架1上。As shown in Figure 1, the high-efficiency quantitative adjustable medium subpackaging equipment of the present utility model includes a medium barrel 3, a peristaltic pump 6, a peristaltic pump head 5, a medium delivery tube 4, a subpackaging tube fixer 9, and a quantitative controller 8 and the controller 7; one end of the culture medium delivery pipe 4 is connected to the culture medium barrel 3, and the other end is fixed to the sub-packaging tube holder 9 through the peristaltic pump head 5; one end of the quantitative controller 8 is connected with the peristaltic pump 6, and the other end It is connected with the controller 7, and the other end of the controller 7 is connected with the peristaltic pump 6; the culture medium bucket 3 is provided with a liftable automatic rotating agitator 2, and the automatic rotating agitator 2 is fixed on the support 1.

培养基桶3材质不限,可直接购买一定大小的塑料桶、铝桶或不锈钢桶均可,可直接置于地板或试验台上,自动旋转搅拌器2固定在支架1上,可升降;蠕动泵6通过电源开关控制,通过其上的旋转钮指示的数字调速,带有控制器插孔;蠕动泵头5通过串联方式连接到蠕动泵上,数量可增减,统一由蠕动泵6控制,保证每个蠕动泵头蠕动一致;蠕动泵头带有输送管卡口,每个泵头连接1条培养基输送管;培养基输送管4长短可增减,一端放入培养基桶,一端根据需要固定在分装管固定器;分装管固定器9可以利用有机玻璃制作,便于观察分装过程,可根据管的数量、容器的大小确定管间距离和管数,带手柄,便于抓握;定量控制器8可设定自动或手动功能,并通过调节运行与间隔时间实现自动定量,或者调节运行时间,连接控制器7,实现手动定量;控制器7连接蠕动泵6,可根据需要改装为脚踏或手控。The material of culture medium barrel 3 is not limited, you can directly buy plastic barrels, aluminum barrels or stainless steel barrels of a certain size, and can be placed directly on the floor or on the test bench. The automatic rotating stirrer 2 is fixed on the support 1 and can be lifted; The pump 6 is controlled by a power switch, and the digital speed is indicated by the rotary knob on it, with a controller jack; the peristaltic pump head 5 is connected to the peristaltic pump in series, and the number can be increased or decreased, and is controlled by the peristaltic pump 6 , to ensure consistent peristalsis of each peristaltic pump head; the peristaltic pump head has a delivery tube bayonet, and each pump head is connected to a medium delivery tube; the length of the medium delivery tube 4 can be increased or decreased, one end is put into the medium bucket, Fix it on the sub-package tube holder as required; the sub-package tube fixer 9 can be made of plexiglass, which is convenient for observing the sub-package process. The distance between the tubes and the number of tubes can be determined according to the number of tubes and the size of the container. It has a handle for easy grasping control; quantitative controller 8 can set automatic or manual function, and realize automatic quantitative by adjusting operation and interval time, or adjust running time, connect controller 7 to realize manual quantitative; controller 7 is connected with peristaltic pump 6, can be adjusted according to needs Converted to pedal or hand control.

本实用新型高效定量可调培养基分装设备工作过程如下:首先将配制好的培养基装入培养基桶3,开动搅拌器,使培养基混匀。将培养基输送管4(若干条)放入培养基底部,设定蠕动泵6运行速度(在蠕动泵上设定)和运行时间(定量控制器设定),接通电源,蠕动泵带动泵头蠕动,使插入液体中的软管内形成负压状态,将液体吸入软管内,多次蠕动,当软管内充满了液体后,再次运行,吸入的液体便通过培养基输送管、分装管固定器,注入排列好的容器中,从而一次定量分装多个容器。分装完毕后,在培养基桶3内倒入清水,搅拌,开动连续蠕动,将清水从培养基输送管放入到废液桶中,可自动清洗干净,无需拆卸。The working process of the high-efficiency, quantitative and adjustable medium subpackaging equipment of the utility model is as follows: firstly, the prepared medium is put into the medium barrel 3, and the stirrer is started to mix the medium evenly. Put the culture medium delivery pipe 4 (several pieces) into the bottom of the culture medium, set the running speed of the peristaltic pump 6 (set on the peristaltic pump) and running time (set by the quantitative controller), turn on the power, and the peristaltic pump drives the pump The head is peristaltic to form a negative pressure state in the hose inserted into the liquid. The liquid is sucked into the hose and peristaltic for many times. Fill the tube holder, pour into the arranged containers, so as to quantitatively dispense multiple containers at one time. After subpackaging, pour clean water into the medium bucket 3, stir, start continuous peristalsis, and put clean water from the medium delivery pipe into the waste liquid bucket, which can be cleaned automatically without disassembly.

培养基桶为普通市售塑料桶,容积不限,为10~100升;培养基输送管为耐压硅胶管,长度可根据需要来定;外接电源为常规交流电,电压220V;将培养基分装到玻璃瓶、试管中,分装速度为每分钟60~300个。The medium barrel is an ordinary commercially available plastic barrel with an unlimited volume of 10-100 liters; the medium delivery tube is a pressure-resistant silicone tube, and the length can be determined according to the needs; the external power supply is a conventional alternating current with a voltage of 220V; divide the medium into Put it into glass bottles and test tubes, and the dispensing speed is 60-300 pieces per minute.

以上所揭露的仅为本实用新型的较佳实施例而已,当然不能以此来限定本实用新型之权利范围,因此依本实用新型权利要求所作的等同变化,仍属于本实用新型所涵盖的范围。The above disclosures are only preferred embodiments of the present utility model, and of course the scope of rights of the present utility model cannot be limited by this, so equivalent changes made according to the claims of the present utility model still belong to the scope covered by the present utility model .

Claims (1)

1.一种高效定量可调培养基分装设备,其特征在于:包括培养基桶、蠕动泵、蠕动泵头、培养基输送管、分装管固定器、定量控制器和控制器;所述培养基输送管一端连接培养基桶,经过蠕动泵头,另一端固定到分装管固定器;定量控制器一端与蠕动泵连接,另一端与控制器的一端连接;控制器的另一端再与蠕动泵连接,所述培养基桶内设有可升降的自动旋转搅拌器,自动旋转搅拌器固定在支架上。1. A high-efficiency quantitative adjustable medium subpackaging equipment is characterized in that: it comprises a medium bucket, a peristaltic pump, a peristaltic pump head, a medium delivery tube, a subpackaging tube fixer, a quantitative controller and a controller; One end of the culture medium conveying pipe is connected to the culture medium bucket, passes through the peristaltic pump head, and the other end is fixed to the fixing tube of the dispensing tube; one end of the quantitative controller is connected to the peristaltic pump, and the other end is connected to one end of the controller; the other end of the controller is connected to the The peristaltic pump is connected, and a liftable automatic rotating agitator is arranged in the culture medium bucket, and the automatic rotating agitator is fixed on the support.
CN 201220109170 2012-03-07 2012-03-07 A high-efficiency quantitative and adjustable medium distribution equipment Expired - Fee Related CN202492352U (en)

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CN105642375A (en) * 2015-12-30 2016-06-08 丁永霞 Protein mixing, heating, digesting and volume metering apparatus
CN105667851A (en) * 2016-03-04 2016-06-15 广西壮族自治区农业科学院生物技术研究所 Automatic culture medium split charging device
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CN108587858A (en) * 2018-04-02 2018-09-28 上海曼森生物科技有限公司 Dispense the priming device of culture medium
CN113247846A (en) * 2021-01-27 2021-08-13 中原环保股份有限公司 Manufacturing equipment and manufacturing process of slant culture medium
CN115281041A (en) * 2022-07-05 2022-11-04 吉林省农业科学院 Morchella culture device and using method thereof
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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103766205A (en) * 2014-01-28 2014-05-07 福建兰水金莲生物科技有限公司 Preparation method for anoectochilus roxburghii bottled culture medium and subpackaging device thereof
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CN105667851A (en) * 2016-03-04 2016-06-15 广西壮族自治区农业科学院生物技术研究所 Automatic culture medium split charging device
CN106680437A (en) * 2017-02-28 2017-05-17 富兰克润滑科技(太仓)有限公司 Device for testing foam characteristics of metal working fluid
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CN106978329B (en) * 2017-05-23 2023-07-25 山东省农业科学院玉米研究所 Test tube medium automatic dispensing device
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CN115281041A (en) * 2022-07-05 2022-11-04 吉林省农业科学院 Morchella culture device and using method thereof
CN118556612A (en) * 2024-07-29 2024-08-30 寻甸高原农业科技有限责任公司 MS culture medium filling equipment

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