CN115281041A - Morchella culture device and using method thereof - Google Patents
Morchella culture device and using method thereof Download PDFInfo
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- CN115281041A CN115281041A CN202210790773.9A CN202210790773A CN115281041A CN 115281041 A CN115281041 A CN 115281041A CN 202210790773 A CN202210790773 A CN 202210790773A CN 115281041 A CN115281041 A CN 115281041A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
- A01G18/22—Apparatus for the preparation of culture media, e.g. bottling devices
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Abstract
The invention discloses a morchella culture device and a using method thereof, and the morchella culture device comprises a culture medium preparation box and a culture medium filling box, wherein the culture medium preparation box is arranged at the upper side of the culture medium filling box, a support column is arranged in the middle of the culture medium preparation box, an electric heating plate is arranged at the bottom of an inner cavity of the culture medium preparation box, one side of the culture medium preparation box is inwards sunken to form a filling equipment mounting groove, a lifting mechanism is arranged on the side wall of the filling equipment mounting groove, and filling equipment is arranged at one side of the lifting mechanism and positioned in the filling equipment mounting groove; can carry out the culture medium automatic loading of test tube and culture dish according to the user demand, it is convenient to switch, and high-efficient safety, and need not to carry out manual loading, has avoided culture medium contact external environment to cause the problem of pollution, need not to carry out high-pressure steam sterilization to the culture medium after filling.
Description
Technical Field
The invention belongs to the technical field of morel culture, and particularly relates to a morel culture device and a using method thereof.
Background
In the prior art, the process of culturing the morchella comprises the steps of firstly adding water into a culture medium raw material required by the morchella culture, boiling the raw material, subpackaging the boiled raw material onto a culture dish or a test tube, plugging a cotton plug, placing the sterilized raw material into an autoclave for sterilization, and carrying out inoculation on a morchella strain after condensation; the split charging is carried out manually after the preparation of the culture medium, and the split charging is carried out in a pressure sterilizer manually for sterilization, so that the efficiency is low, the culture medium is easy to be polluted in the manual operation process, the follow-up high-pressure sterilization is required, and the operation is very inconvenient.
Disclosure of Invention
The invention aims to provide a morchella culture device and a using method thereof, and aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a morchella culture device comprises a culture medium preparation box and a culture medium filling box, wherein the culture medium preparation box is arranged on the upper side of the culture medium filling box, a support column is arranged in the middle of the culture medium preparation box, an electric heating plate is arranged at the bottom of an inner cavity of the culture medium preparation box, one side of the culture medium preparation box is inwards sunken to form a filling equipment mounting groove, an elevating mechanism is arranged on the side wall of the filling equipment mounting groove, and filling equipment is arranged on one side of the elevating mechanism and located in the filling equipment mounting groove;
the filling equipment comprises a main pipe, branch pipes, hoses, hard pipes, a culture medium filling box, an injection head and a test tube filling box, wherein two ends of the main pipe are respectively connected with the bottom of the culture medium preparation box and the branch pipes, the branch pipes are also connected with a plurality of hoses, each hose is respectively connected with one hard pipe, the output end of the lifting mechanism penetrates through the upper end face of the culture medium filling box and is fixedly connected with the culture medium filling box, the hard pipes penetrate through the upper end face of the culture medium filling box and are communicated with the culture medium filling box, and the injection head is arranged on the lower end face of the culture medium filling box;
the utility model discloses a culture medium loading box, including culture medium loading box, rotor plate, drive motor, cell culture medium loading box, cell culture medium lid, cell culture medium loading box inside wall all rotates and sets up the rotor plate, the outer wall installation driving motor of culture medium loading box, driving motor connects the rotor plate through the pivot drive, rotate between the rotor plate and set up a plurality of groups and place the box, place the interior culture dish of placing of box, set up the culture dish lid on the culture dish, the culture dish is covered and is set up foraminiferous sealing plug, and the bottom of placing the box outermost end of every group is still leveled and is set up locking screw.
Preferably, the upper end of the culture medium preparation box is rotatably provided with a preparation box cover plate, and a stirrer is arranged inside the culture medium preparation box.
Preferably, the lifting mechanism is provided as a cylinder.
Preferably, the shape of the injection head is set to be conical, and an inlet at the upper end of the injection head is provided with an electromagnetic valve.
Preferably, the manifold is fitted with a pump.
Preferably, the placing boxes of each group are connected end to end, and the outermost ends of the placing boxes are rotatably connected with the rotating plate.
Preferably, the side surface of the culture medium filling box is rotatably provided with a filling box cover plate.
Preferably, the bottom of the culture medium filling box is provided with a water tank, an electric heater is arranged in the water tank, and the culture medium filling box is further provided with a pressure release valve and a pressure gauge.
Preferably, place the bottom of the box and set up a plurality of through-holes, place the inside test-tube rack that sets up of box, the last test tube of pegging graft of test-tube rack, the test tube upper end sets up foraminiferous sealing plug, the lower extreme of test tube passes the through-hole, elevating system's output passes culture medium and loads the up end and the fixed connection test tube of case and loads the box, the hard tube passes the up end of culture medium and loads the box and communicate the test tube, the lower terminal surface that the test tube loaded the box sets up the injection head.
A use method of a morchella culture device comprises the following steps:
1) Adding a culture medium raw material into a culture medium preparation box, heating for preparation, and preparing for later use;
2) Installing a culture medium filling box or a test tube filling box at the lower end of the lifting mechanism according to requirements, and communicating the hard tube with the corresponding culture medium filling box or test tube filling box;
3) Placing a culture dish in a placing box corresponding to the culture medium filling box, placing a test tube rack in a placing box corresponding to the test tube filling box, and placing a test tube on the test tube rack;
4) Starting a pump, inputting the prepared culture medium in the step 1) into a culture medium filling box and a test tube filling box through a main pipe, a branch pipe, a hose and a hard pipe in sequence, and descending a lifting mechanism to ensure that an injection head at the lower end of the culture medium filling box is inserted into a perforated sealing plug on a culture dish cover, an injection head at the lower end face of the test tube filling box is inserted into a perforated sealing plug at the upper end of a test tube to inject the culture medium into the culture dish and the test tube, wherein after injection is finished, the lifting mechanism drives the culture medium filling box and the test tube filling box to reset;
5) Starting a driving motor, driving the rotation plate to rotate by the driving motor, so that the culture dishes on the next group of placing boxes are shifted to the lower side of the culture medium filling box, the test tubes on the next group of placing boxes are shifted to the lower side of the test tube filling box, and repeating the step 4) to continue injection, thereby completing the filling of all the culture dishes and the test tubes.
The invention has the technical effects and advantages that: can carry out the culture medium automatic loading of test tube and culture dish according to the user demand, it is convenient to switch, and high-efficient safety, and need not to carry out manual loading, has avoided culture medium contact external environment to cause the problem of pollution, need not to carry out high-pressure steam sterilization to the culture medium after filling.
Drawings
FIG. 1 is a schematic view of the structure of the present invention;
FIG. 2 is a schematic view of the construction of the filling apparatus of the present invention;
FIG. 3 is a schematic view of the structure of the culture dish of the present invention;
FIG. 4 is a schematic view of the structure of the placing box of the present invention;
fig. 5 is a schematic view of the structure of the test tube rack and test tubes of the present invention.
In the figure: 1. a culture medium preparation box; 2. a culture medium filling box; 3. preparing a box cover plate; 4. a filling equipment mounting groove; 5. a lifting mechanism; 6. a filling device; 61. a main pipe; 62. pipe distribution; 63. a hose; 64. a hard tube; 65. a culture medium loading cassette; 66. an injection head; 67. a test tube loading cassette; 68. a pump; 7. a drive motor; 8. a rotating plate; 9. placing the box; 10. a culture dish; 11. a test tube rack; 12. filling a box cover plate; 13. a culture dish cover; 14. a sealing plug with a hole; 15. test tubes.
Detailed Description
The following further describes embodiments of the present invention with reference to the drawings. It should be noted that the description of the embodiments is provided to help understanding of the present invention, and is not intended to limit the present invention. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Example one
A morchella culture apparatus as shown in fig. 1-4, comprising a culture medium preparation box 1 and a culture medium filling box 2, wherein a preparation box cover plate 3 is rotatably arranged at the upper end of the culture medium preparation box 1, a stirrer is arranged inside the culture medium preparation box 1, the stirrer can be used for stirring added culture medium raw materials, a filling box cover plate 12 is rotatably arranged on the side surface of the culture medium filling box 2, the culture medium preparation box 1 is arranged at the upper side of the culture medium filling box 2, and a support column is arranged in the middle of the culture medium preparation box 1 and used for separating the culture medium preparation box 1 from the culture medium filling box 2, so as to avoid mutual influence of heat transfer, an electric heating plate is arranged at the bottom of an inner cavity of the culture medium preparation box 1, the culture medium raw materials of the culture medium preparation box 1 are heated and boiled by the electric heating plate, a filling equipment mounting groove 4 is formed by one side of the culture medium preparation box 1 being concave inwards, a lifting mechanism 5 is arranged on the side wall of the filling equipment mounting groove 4, the lifting mechanism 5 is arranged as an air cylinder, and a filling equipment 6 is arranged in the filling equipment mounting groove 4 at one side of the lifting mechanism 5;
as shown in fig. 2, the filling device 6 comprises a main pipe 61, branch pipes 62, hoses 63, hard pipes 64, a culture medium filling box 65, an injection head 66 and a test tube filling box 67, wherein two ends of the main pipe 61 are respectively connected with the bottom of the culture medium preparation box 1 and the branch pipes 62, a pump 68 is mounted on the main pipe 61 and provides power for the input of culture medium, a plurality of hoses 63 are further connected on the branch pipes 62, each hose 63 is respectively connected with one hard pipe 64, the output end of the lifting mechanism 5 penetrates through the upper end face of the culture medium filling box 2 and is fixedly connected with the culture medium filling box 65, the hard pipes 64 penetrate through the upper end face of the culture medium filling box 2 and are communicated with the culture medium filling box 65, the injection head 66 is arranged on the lower end face of the culture medium filling box 65, the injection head 66 is tapered in shape, and an inlet position on the upper end of the injection head 66 is provided with an electromagnetic valve for controlling the on-off of the injection of culture medium;
2 inside walls of culture medium filling box all rotate and set up rotor plate 8, the outer wall installation driving motor 7 of culture medium filling box 2, driving motor 7 connects rotor plate 8 through pivot drive, rotate between the rotor plate 8 and set up a plurality of groups and place box 9, the box 9 end to end of placing of every group, and the outermost end rotates with rotor plate 8 to be connected, place and place culture dish 10 in the box 9, set up culture dish lid 13 on the culture dish 10, set up foraminiferous sealing plug 14 on the culture dish lid 13.
The bottom of culture medium filling box 2 sets up the water tank, sets up electric heater in the water tank, still sets up relief valve and manometer on the culture medium filling box 2, through the water in the heating water tank, can carry out high-pressure steam sterilization to 2 insides of culture medium filling box, sterilizes culture dish 10, test-tube rack 11 and test tube 15 and the inside spare part that will add.
Adding culture medium raw materials into the culture medium preparation box 1, heating preparation, starting the pump 68 after preparation, through passing through the header pipe 61 in proper order, the branch pipe 62, the hose 63, the hard tube 64 inputs culture medium to the culture medium filling box 65, simultaneously, the elevating system 5 descends, make the injection head 66 of culture medium filling box 65 lower extreme insert in the foraminiferous sealing plug 14 on the culture dish lid 13, pour into culture medium into the culture dish 10, after the injection is accomplished, the elevating system 5 drives the culture medium filling box 65 and resets, driving motor 7 drives the rotor plate 8 and rotates, make the next group place culture dish 10 on the box 9 and change over to the downside of culture medium filling box 65, continue the injection, accomplish the loading of all culture dishes 10.
Example two
Based on the first embodiment, as shown in fig. 5, a plurality of through holes are formed in the bottom of the placing box 9, a test tube rack 11 is arranged in the placing box 9, a test tube 15 is inserted on the test tube rack 11, a sealing plug 14 with a hole is arranged at the upper end of the test tube 15, the lower end of the test tube 15 penetrates through the through holes, the output end of the lifting mechanism 5 penetrates through the upper end surface of the culture medium filling box 2 and is fixedly connected with a test tube filling box 67, a hard tube 64 penetrates through the upper end surface of the culture medium filling box 2 and is communicated with the test tube filling box 67, and an injection head 66 is arranged on the lower end surface of the test tube filling box 67; replace culture medium cassette 65 for test tube cassette 67 to with test tube cassette 67 and hard tube 64 intercommunication, the test tube rack 11 is put into and is placed in the box 9, and put into test tube 15 on the test tube rack 11, start elevating system 5 and pump 68, the foraminiferous sealing plug 14 of test tube 15 upper end is inserted to the injection head 66 of test tube cassette 67 lower extreme face, can carry out the injection simultaneously to test tube 15 on the whole test tube rack 11.
The bottom of 9 outermost ends of box of placing of every group still the level set up locking screw, need the slope to place the test tube after the culture medium is packed into in the test tube 15, can screw up locking screw, will place box 9 and rotate to inclination and fix.
The upper wall of culture medium filling box 2 sets up the accommodation space of culture medium filling box 65 and test tube filling box 67, and elevating system 5 drives the culture medium filling box 65 and test tube filling box 67 back that resets, can arrange in this accommodation space, does not influence the rotation switching of placing box 9.
This hickory chick culture apparatus can carry out the culture medium of test tube 15 and culture dish 10 according to the user demand and load at the in-process that uses, and it is convenient to switch, and need not to carry out manual loading, high-efficient safety.
The invention also provides a using method of the morel culture device, which comprises the following steps:
step one, adding a culture medium raw material into a culture medium preparation box 1, heating for preparation, and preparing for later use;
step two, installing a culture medium filling box 65 or a test tube filling box 67 at the lower end of the lifting mechanism 5 according to requirements, and communicating the hard tube 64 with the corresponding culture medium filling box 65 or test tube filling box 67;
placing a culture dish 10 in a placing box 9 corresponding to the culture medium filling box 65, placing a test tube rack 11 in a placing box 9 corresponding to the test tube filling box 67, and placing a test tube 15 on the test tube rack 11;
step four, starting a pump 68, inputting the culture medium prepared in the step one into the culture medium filling box 65 and the test tube filling box 67 through a main pipe 61, a branch pipe 62, a hose 63 and a hard pipe 64 in sequence, and simultaneously descending the lifting mechanism 5 to ensure that an injection head 66 at the lower end of the culture medium filling box 65 is inserted into a perforated sealing plug 14 on the culture dish cover 13, an injection head 66 at the lower end face of the test tube filling box 67 is inserted into a perforated sealing plug 14 at the upper end of a test tube 15 to inject the culture medium into the culture dish 10 and the test tube 15, and after the injection is finished, the lifting mechanism 5 drives the culture medium filling box 65 and the test tube filling box 67 to reset;
and step five, starting the driving motor 7, driving the rotating plate 8 to rotate by the driving motor 7, enabling the culture dishes 10 on the next group of placing boxes 9 to be transferred to the lower side of the culture medium filling box 65, enabling the test tubes 15 on the next group of placing boxes 9 to be transferred to the lower side of the test tube filling box 67, repeating the step four, continuing injection, and completing filling of all the culture dishes 10 and the test tubes 15.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (10)
1. The utility model provides a hickory chick culture apparatus, includes culture medium preparation case (1) and culture medium filling box (2), upside and the centre that culture medium filling box (2) was installed in culture medium preparation case (1) sets up support column, its characterized in that: an electric heating plate is arranged at the bottom of an inner cavity of the culture medium preparation box (1), one side of the culture medium preparation box (1) is inwards sunken to form a filling equipment installation groove (4), a lifting mechanism (5) is installed on the side wall of the filling equipment installation groove (4), and filling equipment (6) is installed on one side of the lifting mechanism (5) and located in the filling equipment installation groove (4);
the filling equipment (6) comprises a main pipe (61), branch pipes (62), hoses (63), hard pipes (64), culture medium filling boxes (65), injection heads (66) and test tube filling boxes (67), wherein the two ends of the main pipe (61) are respectively connected with the bottoms of the culture medium preparation boxes (1) and the branch pipes (62), the branch pipes (62) are further connected with a plurality of hoses (63), each hose (63) is respectively connected with one hard pipe (64), the output end of the lifting mechanism (5) penetrates through the upper end face of each culture medium filling box (2) and is fixedly connected with the culture medium filling boxes (65), each hard pipe (64) penetrates through the upper end face of each culture medium filling box (2) and is communicated with the corresponding culture medium filling box (65), and the injection head (66) is arranged on the lower end face of each culture medium filling box (65);
culture medium loads case (2) inside wall and all rotates and sets up rotor plate (8), culture medium loads outer wall installation driving motor (7) of case (2), driving motor (7) are connected rotor plate (8) through the pivot drive, rotate between rotor plate (8) and set up a plurality of groups and place box (9), place culture dish (10) in box (9), set up culture dish lid (13) on culture dish (10), set up foraminiferous sealing plug (14) on culture dish lid (13), the bottom of placing box (9) the outer end of every group is the level still and is set up locking screw.
2. The morchella culture device according to claim 1, wherein: the upper end of the culture medium preparation box (1) is rotatably provided with a preparation box cover plate (3), and a stirrer is arranged in the culture medium preparation box (1).
3. The morchella culture device according to claim 1, wherein: the lifting mechanism (5) is arranged as an air cylinder.
4. The morchella culture device according to claim 1, wherein: the shape of the injection head (66) is set to be conical, and an inlet position at the upper end of the injection head (66) is provided with an electromagnetic valve.
5. The morchella culture device according to claim 1, wherein: the manifold (61) is provided with a pump (68).
6. The morchella culture apparatus according to claim 1, wherein: the placing boxes (9) of each group are connected end to end, and the outermost ends of the placing boxes are rotationally connected with the rotating plate (8).
7. The morchella culture device according to claim 1, wherein: and a filling box cover plate (12) is rotatably arranged on the side surface of the culture medium filling box (2).
8. The morchella culture apparatus according to claim 1, wherein: the bottom of culture medium filling box (2) sets up the water tank, set up electric heater in the water tank, still set up relief valve and manometer on culture medium filling box (2).
9. The morchella culture device according to claim 1, wherein: place box (9) bottom and set up a plurality of through-holes, place inside test-tube rack (11) that sets up of box (9), test tube (15) of pegging graft on test-tube rack (11), test tube (15) upper end sets up foraminiferous sealing plug (14), the through-hole is passed to the lower extreme of test tube (15), the output of elevating system (5) passes culture medium and loads up the up end and the fixed connection test tube of case (2) and loads box (67), hard tube (64) pass the up end that culture medium loaded case (2) and communicate the test tube and load box (67), the lower terminal surface that the test tube loaded box (67) sets up injection head (66).
10. The use method of the morchella culture device is characterized by comprising the following steps:
1) Adding a culture medium raw material into the culture medium preparation box (1), heating for preparation, and preparing for later use;
2) A culture medium filling box (65) or a test tube filling box (67) is arranged at the lower end of the lifting mechanism (5) according to requirements, and the hard tube (64) is communicated with the corresponding culture medium filling box (65) or test tube filling box (67);
3) Placing a culture dish (10) in a placing box (9) corresponding to the culture medium filling box (65), placing a test tube rack (11) in a placing box (9) corresponding to the test tube filling box (67), and placing a test tube (15) on the test tube rack (11);
4) Starting a pump (68), inputting the culture medium prepared in the step 1 to a culture medium filling box (65) and a test tube filling box (67) through a main pipe (61), a branch pipe (62), a hose (63) and a hard pipe (64) in sequence, and simultaneously descending a lifting mechanism (5) to ensure that an injection head (66) at the lower end of the culture medium filling box (65) is inserted into a hole-shaped sealing plug (14) on a culture dish cover (13), an injection head (66) at the lower end surface of the test tube filling box (67) is inserted into a hole-shaped sealing plug (14) at the upper end of a test tube (15), injecting the culture medium into a culture dish (10) and the test tube (15), and after injection is finished, driving the culture medium filling box (65) and the test tube filling box (67) to reset by the lifting mechanism (5);
5) And (3) starting the driving motor (7), driving the rotating plate (8) to rotate by the driving motor (7), so that the culture dishes (10) on the next group of placing boxes (9) are turned to the lower side of the culture medium filling box (65), the test tubes (15) on the next group of placing boxes (9) are turned to the lower side of the test tube filling box (67), and repeating the step (4) to continue injection to finish the filling of all the culture dishes (10) and the test tubes (15).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210790773.9A CN115281041A (en) | 2022-07-05 | 2022-07-05 | Morchella culture device and using method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210790773.9A CN115281041A (en) | 2022-07-05 | 2022-07-05 | Morchella culture device and using method thereof |
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| CN115281041A true CN115281041A (en) | 2022-11-04 |
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| CN202210790773.9A Pending CN115281041A (en) | 2022-07-05 | 2022-07-05 | Morchella culture device and using method thereof |
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