CN210143514U - Lily tissue culture medium filling device - Google Patents
Lily tissue culture medium filling device Download PDFInfo
- Publication number
- CN210143514U CN210143514U CN201920884860.4U CN201920884860U CN210143514U CN 210143514 U CN210143514 U CN 210143514U CN 201920884860 U CN201920884860 U CN 201920884860U CN 210143514 U CN210143514 U CN 210143514U
- Authority
- CN
- China
- Prior art keywords
- liquid outlet
- culture medium
- flow control
- quantitative
- mixing tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
技术领域technical field
本实用新型涉及植物组织培养装置领域,特别是涉及一种百合组培培养基灌装装置。The utility model relates to the field of plant tissue culture devices, in particular to a filling device for lily tissue culture medium.
背景技术Background technique
传统的百合繁殖是靠播种、扦插、分株、压条、嫁接这些措施来实现的,然而在花卉业迅猛发展的今天,传统的育种技术已经无法满足生产需要,因而人们对百合的繁殖技术提出了更高的要求。利用组织培养进行百合繁殖的技术日趋成熟。目前百合育种可以利用组织培养为其提供种苗。The traditional breeding of lily is achieved by means of sowing, cuttings, ramets, layering and grafting. However, with the rapid development of the flower industry today, traditional breeding techniques have been unable to meet the needs of production. higher requirement. The technology of lily propagation by tissue culture is becoming more and more mature. At present, lily breeding can use tissue culture to provide seedlings for it.
在组织培养中会用到培养基,现有技术中是配制培养基后,通过手动倒入培养皿或者培养瓶中,效率低下,中国专利(专利号CN206750308U)公开了一种用于百合培养基的灌装装置,在出液口连接出液总管,出液总管通过转换头连接多个分装管的一端,多个分装管的另一端固定在固定架上,在所述固定架上固定有多个灌装头,每个灌装头分别连接一个分装管;Medium is used in tissue culture. In the prior art, after the medium is prepared, it is manually poured into a petri dish or a culture bottle, which is inefficient. Chinese patent (patent number CN206750308U) discloses a medium for lily. The filling device is connected to a liquid outlet pipe, which is connected to one end of a plurality of sub-packaging pipes through a conversion head, and the other ends of the plurality of sub-packaging pipes are fixed on a fixing frame, which is fixed on the fixing frame There are multiple filling heads, each filling head is connected with a dispensing tube;
多个灌装头可以同时完成多个培养瓶的灌装,提高了工作效率。Multiple filling heads can complete the filling of multiple culture bottles at the same time, which improves work efficiency.
但是在组培实验中,需要每个培养单元的条件均统一,上述装置无法进行定量灌装,且控制不够方便。However, in the tissue culture experiment, the conditions of each culture unit need to be uniform, the above-mentioned device cannot perform quantitative filling, and the control is not convenient enough.
实用新型内容Utility model content
本实用新型旨在提供一种百合组培培养基灌装装置,以解决上述背景技术中的问题。The utility model aims to provide a lily tissue culture medium filling device to solve the above-mentioned problems in the background technology.
为实现上述目的,本实用新型采用如下技术方案:To achieve the above object, the utility model adopts the following technical solutions:
一种百合组培培养基灌装装置,包括培养基混合罐、位于培养基混合罐底部的支架,所述培养基混合罐顶面设有开口,且所述开口处设置有罐盖,所述培养基混合罐的内设置有搅拌装置,所述培养基混合罐的外壁设置有加热套,所述培养基混合罐的底部开设有出液口,所述出液口上设置有浮球阀,所述出液口与所述浮球阀的进水口连通,所述支架为u形结构,所述支架的两个侧壁之间水平设有u形槽,所述u形槽内设置有定量分装机构,所述支架的中部水平且位于所述出液口下方处设置有定量分装机构,所述定量分装机构包括若干互体积和形状均相同的定量槽,相邻的所述定量槽共用侧壁上均在同一高度处设置有开孔,所述浮球阀的出水口通过第一出液管与其中一个所述定量槽连通,所述第一出液管上设置有第一流量控制阀,所述浮球阀的浮球设置于另一个所述定量槽内,每个所述定量槽的底部均设置有第二出液管,且每个所述第二出液管的底端均穿过所述u形槽的底面,每个所述第二出液管上、且位于u 形槽下方处均设置有第二流量控制阀,所述第二出液管的下方对应设置有用于放置培养瓶的放置槽,且所述放置槽安装在所述支架上。A lily tissue culture medium filling device, comprising a medium mixing tank and a support located at the bottom of the medium mixing tank, the top surface of the medium mixing tank is provided with an opening, and the opening is provided with a tank cover, and the The medium mixing tank is provided with a stirring device, the outer wall of the medium mixing tank is provided with a heating jacket, the bottom of the medium mixing tank is provided with a liquid outlet, and the liquid outlet is provided with a floating ball valve. The liquid outlet is communicated with the water inlet of the floating ball valve, the bracket is a u-shaped structure, a u-shaped groove is horizontally arranged between two side walls of the bracket, and a quantitative dispensing mechanism is arranged in the u-shaped groove , the middle part of the support is horizontal and is located below the liquid outlet with a quantitative dispensing mechanism, the quantitative dispensing mechanism includes a number of quantitative grooves with the same mutual volume and shape, and the adjacent quantitative grooves share a side The walls are all provided with openings at the same height, the water outlet of the floating ball valve is communicated with one of the quantitative grooves through a first liquid outlet pipe, and the first liquid outlet pipe is provided with a first flow control valve, The floating ball of the floating ball valve is arranged in another quantitative tank, the bottom of each quantitative tank is provided with a second liquid outlet pipe, and the bottom end of each second liquid outlet pipe passes through On the bottom surface of the u-shaped groove, a second flow control valve is provided on each of the second liquid outlet pipes and below the u-shaped groove, and a corresponding valve for placing culture is provided below the second liquid outlet pipe. Placement slot for the bottle, and the placement slot is mounted on the bracket.
进一步的,所述搅拌装置包括螺旋叶片和旋转电机,所述旋转电机位于所述培养基混合罐顶部,且所述旋转电机的输出轴穿过所述培养基混合罐的顶部并固接有加长杆、且所述加长杆的底端连接有所述螺旋叶片。Further, the stirring device includes a helical blade and a rotary motor, the rotary motor is located at the top of the culture medium mixing tank, and the output shaft of the rotating motor passes through the top of the culture medium mixing tank and is fixed with a lengthening A rod, and the bottom end of the extension rod is connected with the helical blade.
进一步的,所述第一出液管的下管口高于所述开孔的高度。Further, the lower nozzle of the first liquid outlet pipe is higher than the height of the opening.
进一步的,所述定量分装机构外设置有保温棉。Further, thermal insulation cotton is provided outside the quantitative dispensing mechanism.
进一步的,所述第一流量控制阀和第二控流量制阀均为电磁阀,所述支架上还设置有控制器,所述第一流量控制阀、第二流量控制阀均与所述控制器电性连接,且每个第二流量控制阀均为串联。Further, the first flow control valve and the second flow control valve are both solenoid valves, the support is also provided with a controller, and the first flow control valve and the second flow control valve are both connected with the control valve. The devices are electrically connected, and each second flow control valve is connected in series.
与现有技术相比,本实用新型具有如下技术效果:Compared with the prior art, the utility model has the following technical effects:
1.通过设置加热套使培养基保持液体状态,通过搅拌装置使培养基保持均匀。1. The medium is kept in a liquid state by setting the heating mantle, and the medium is kept uniform by the stirring device.
2.通过设置定量分装机构,能够同时灌装多个培养基,且能够保证每个培养基的定量分装均匀,通过设置浮球阀,使其自动定量,操作便捷。2. By setting the quantitative dispensing mechanism, multiple media can be filled at the same time, and the quantitative dispensing of each medium can be ensured evenly. By setting the floating ball valve, it can be automatically quantitative and easy to operate.
附图说明Description of drawings
图1为本实用新型结构示意图。Figure 1 is a schematic structural diagram of the utility model.
图2为本实用新型的定量分装机构的结构示意图。FIG. 2 is a schematic structural diagram of the quantitative dispensing mechanism of the present invention.
图中:1-培养基混合罐,2-出液口,3-罐盖,4-加热套,5-浮球阀,6-支架, 7-定量槽,8-第二出液管,9-第一出液管,10-第二流量控制阀,11-放置槽,12- 旋转电机,13-螺旋叶片,14-第一流量控制阀,15-控制器。In the picture: 1- Medium mixing tank, 2- Liquid outlet, 3- Tank cover, 4- Heating jacket, 5- Floating ball valve, 6- Bracket, 7- Quantitative tank, 8- Second liquid outlet pipe, 9- The first liquid outlet pipe, 10-second flow control valve, 11-placement tank, 12-rotating motor, 13-spiral blade, 14-first flow control valve, 15-controller.
具体实施方式Detailed ways
下面结合附图,对本实用新型的一个具体实施方案进行详细描述,但应当理解,本实用新型的保护范围并不受具体实施方式的限制。A specific embodiment of the present invention will be described in detail below with reference to the accompanying drawings, but it should be understood that the protection scope of the present invention is not limited by the specific embodiment.
实施例1Example 1
参见附图1-2,一种百合组培培养基灌装装置,包括培养基混合罐1、位于培养基混合罐1底部的支架6,培养基混合罐1顶部具有开口,且开口处铰接有罐盖3,培养基混合罐1的内设置有搅拌装置,搅拌装置包括旋转电机12 和螺旋叶片13,旋转电机12位于培养基混合罐1上顶面,且旋转电机12的输出轴穿过培养基混合罐1的顶面并固接有加长杆、且加长杆底端螺接有螺旋叶片13,方便将培养基搅拌均匀;Referring to the accompanying drawings 1-2, a lily tissue culture medium filling device, comprising a medium mixing tank 1, a
培养基混合罐1的外壁设置有加热套4,本实施例的加热套4为新瑞琪电热设备有限公司的柔性电加热套,具有升温快、保温好、发热均匀、热效率高等,温控系统具有智能PID控制,精确控温,数显工作温度,培养基混合罐1 的底部开设有出液口2,可以使培养基通过自身重力流出,出液口2上设置有浮球阀5,本实施例的浮球阀5为维成DN8F浮动球球阀,包括进水口、出水口、浮球、连杆、支架和球阀,出液口2与浮球阀5的进水口连通,培养基混合罐1的底部还设置有支架6,支架4为u形,支架的两个侧壁之间水平设有 u形槽,u形槽内设置有定量分装机构,设置支架6的作用是使培养基混合罐 1腾空,便于出液,也是为了能够在底部设置定量分装机构。The outer wall of the culture medium mixing tank 1 is provided with a heating jacket 4. The heating jacket 4 in this embodiment is a flexible electric heating jacket from Xinruiqi Electric Heating Equipment Co., Ltd., which has the advantages of fast heating, good heat preservation, uniform heating, high thermal efficiency, and a temperature control system. It has intelligent PID control, precise temperature control, and digital display of working temperature. The bottom of the medium mixing tank 1 is provided with a
支架6的中部且位于出液口2下方处水平设置有定量分装机构,定量分装机构包括若干体积和形状均相同的定量槽7,本实施例中,定量槽7的数量为 10个,如图2所示,相邻的定量槽7共用的侧壁上均在同一高度处设置有开孔,浮球阀5的出液口通过第一出液管9与其中一个定量槽7连通,第一出液管9 上设置有第一流量控制阀,浮球阀5的浮球设置于另一个定量槽7,每个定量槽7的底部均连通设置有第二出液管8,且每个第二出液管8的底端均穿过u 形槽的底面,每个第二出液管8上、且位于所述u形槽下方处均设置有第二流量控制阀10,第二出液管8的下方对应设置有用于放置培养瓶的放置槽11,且放置槽11安装在支架6上。The middle part of the
本实施例中,第一出液管9的下管口高于开孔的高度,防止第一出液管9 浸入液面,使定量槽7中培养基的体积虚高;定量分装机构外设置有保温棉,防止培养基凝固。In this embodiment, the lower orifice of the first
为了控制方便,本实施例中,第一流量控制阀14和第二流量控制阀10均为电磁阀,支架6上还设置有控制器15,控制器型号为STC859C52,第一流量控制阀14、第二流量控制阀10均与控制器15电性连接,且每个第二流量控制阀10均为串联,控制器上设置有用于控制第一流量控制阀14的第一开关和控制第二流量控制阀10的第二开关For the convenience of control, in this embodiment, the first
本实施例的一种百合培养基灌装装置的工作原理:The working principle of a lily culture medium filling device of the present embodiment:
打开罐盖3,向培养基混合罐中加入待分装的培养基,打开加热套4和旋转电机12,使培养基保持一定的温度和保持均匀,通过第一开关打开第一流量控制阀14,使培养基通过第一出液管进入一个定量槽7,当该定量槽7中的培养基液面高度升高至开孔的高度时,培养基通过开孔流向其它的定量槽7,直至每个定量槽7中的液面高度均相等,且略高于开孔的高度(通过预先设置浮球阀5的位置,使浮球到达该高度时,浮球阀5关闭),此时浮球随着液面上升,自动将浮球阀5关闭,然后通过第一开关关闭第一流量控制阀14,通过第二开关开启第二流量控制阀10,向培养瓶中注入培养基,达到定量灌装的目的,灌装完毕,关闭第二流量控制阀10,打开第一流量控制阀14向定量槽7中注培养基,进行下一批次的操作。Open the
以上的仅为本实用新型的一个实施例,但是本实用新型实施例并非仅限于此,任何本领域能思之的变化都应落在本实用新型的保护范围之内。The above is only an embodiment of the present invention, but the embodiment of the present invention is not limited to this, and any changes that can be conceived in the field should fall within the protection scope of the present invention.
Claims (5)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920884860.4U CN210143514U (en) | 2019-06-12 | 2019-06-12 | Lily tissue culture medium filling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920884860.4U CN210143514U (en) | 2019-06-12 | 2019-06-12 | Lily tissue culture medium filling device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210143514U true CN210143514U (en) | 2020-03-17 |
Family
ID=69762683
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920884860.4U Expired - Fee Related CN210143514U (en) | 2019-06-12 | 2019-06-12 | Lily tissue culture medium filling device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210143514U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112352676A (en) * | 2020-10-28 | 2021-02-12 | 广西壮族自治区农业科学院 | Multipoint automatic quantitative tissue culture solution stirring and filling machine |
| CN115281041A (en) * | 2022-07-05 | 2022-11-04 | 吉林省农业科学院 | Morchella culture device and using method thereof |
-
2019
- 2019-06-12 CN CN201920884860.4U patent/CN210143514U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112352676A (en) * | 2020-10-28 | 2021-02-12 | 广西壮族自治区农业科学院 | Multipoint automatic quantitative tissue culture solution stirring and filling machine |
| CN115281041A (en) * | 2022-07-05 | 2022-11-04 | 吉林省农业科学院 | Morchella culture device and using method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN210143514U (en) | Lily tissue culture medium filling device | |
| CN102839117A (en) | Hydraulic pressure type two-phase temperature-control methane generating experimental device | |
| WO2022016989A1 (en) | Milk fermentation tank having excellent heat exchange performance | |
| CN206486499U (en) | Honey brewing wine fermentation tank | |
| CN207435459U (en) | A kind of incubator of the automatically replaceable culture solution of medical experiment | |
| CN101333268A (en) | Reactor for EPS | |
| CN203535027U (en) | Sulfide stress experiment device | |
| CN204837933U (en) | Cattle and sheep fodder fermenting installation | |
| CN103543241B (en) | Sulfide stress experiment apparatus and experiment method thereof | |
| CN209322909U (en) | Automatic agitating type bioreactor | |
| CN109224930A (en) | A kind of fire foam process units | |
| CN211734193U (en) | Inorganic fire prevention liquid production filling system | |
| CN204699436U (en) | A kind of industrial crystallization kettle | |
| CN206728896U (en) | The simple mandarin sturgeon fish-egg unsticking device of scientific research | |
| CN209039021U (en) | A kind of plant tissue culture media filling apparatus | |
| CN209311192U (en) | A kind of tissue block embedding device of detachable easy-clean | |
| CN208727422U (en) | A lubricating oil heating device | |
| CN207483760U (en) | A kind of biological medicine fermentation tank | |
| CN209271348U (en) | A kind of blend tank | |
| CN223697469U (en) | Wisdom water affair water supply is with adding medicine device | |
| CN221601716U (en) | Improved stirrer for preparing sodium acetate solution | |
| CN205868999U (en) | Online cleaning device of UNICOM's formula fermentation cylinder | |
| CN220835592U (en) | Non-solidified asphalt production system | |
| CN219646784U (en) | Alcohol precipitation tank | |
| CN217836934U (en) | Wine tank |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200317 Termination date: 20210612 |