CN210143514U - Lily tissue culture medium filling device - Google Patents

Lily tissue culture medium filling device Download PDF

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Publication number
CN210143514U
CN210143514U CN201920884860.4U CN201920884860U CN210143514U CN 210143514 U CN210143514 U CN 210143514U CN 201920884860 U CN201920884860 U CN 201920884860U CN 210143514 U CN210143514 U CN 210143514U
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liquid outlet
culture medium
flow control
quantitative
mixing tank
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陈丽静
张丽
马慧
张轶兵
李薇
王宝华
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Shenyang Agricultural University
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Shenyang Agricultural University
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Abstract

The utility model relates to a plant tissue culture device field especially relates to a lily group banks up culture medium filling device with earth. The structure is as follows: including the culture medium blending tank, be located the support of culture medium blending tank bottom, culture medium blending tank top has the opening, the opening part is provided with the cover, be provided with agitating unit in the culture medium blending tank, be provided with the heating jacket on the outer wall of blending medium culturing tank, the liquid outlet has been seted up to the bottom surface of blending medium culturing tank, the intercommunication is provided with the ball-cock assembly on the liquid outlet, the bottom of culture medium blending tank still is provided with the support, the level is equipped with the u groove between two lateral walls of support, the U inslot is provided with quantitative split charging mechanism, the bottom correspondence of quantitative split charging mechanism is provided with the standing groove that is used for placing the. The utility model discloses a lily breeding culture medium filling device can a plurality of culture mediums of filling simultaneously, can realize quantitative filling, high-efficient and simple operation.

Description

一种百合组培培养基灌装装置A kind of lily tissue culture medium filling device

技术领域technical field

本实用新型涉及植物组织培养装置领域,特别是涉及一种百合组培培养基灌装装置。The utility model relates to the field of plant tissue culture devices, in particular to a filling device for lily tissue culture medium.

背景技术Background technique

传统的百合繁殖是靠播种、扦插、分株、压条、嫁接这些措施来实现的,然而在花卉业迅猛发展的今天,传统的育种技术已经无法满足生产需要,因而人们对百合的繁殖技术提出了更高的要求。利用组织培养进行百合繁殖的技术日趋成熟。目前百合育种可以利用组织培养为其提供种苗。The traditional breeding of lily is achieved by means of sowing, cuttings, ramets, layering and grafting. However, with the rapid development of the flower industry today, traditional breeding techniques have been unable to meet the needs of production. higher requirement. The technology of lily propagation by tissue culture is becoming more and more mature. At present, lily breeding can use tissue culture to provide seedlings for it.

在组织培养中会用到培养基,现有技术中是配制培养基后,通过手动倒入培养皿或者培养瓶中,效率低下,中国专利(专利号CN206750308U)公开了一种用于百合培养基的灌装装置,在出液口连接出液总管,出液总管通过转换头连接多个分装管的一端,多个分装管的另一端固定在固定架上,在所述固定架上固定有多个灌装头,每个灌装头分别连接一个分装管;Medium is used in tissue culture. In the prior art, after the medium is prepared, it is manually poured into a petri dish or a culture bottle, which is inefficient. Chinese patent (patent number CN206750308U) discloses a medium for lily. The filling device is connected to a liquid outlet pipe, which is connected to one end of a plurality of sub-packaging pipes through a conversion head, and the other ends of the plurality of sub-packaging pipes are fixed on a fixing frame, which is fixed on the fixing frame There are multiple filling heads, each filling head is connected with a dispensing tube;

多个灌装头可以同时完成多个培养瓶的灌装,提高了工作效率。Multiple filling heads can complete the filling of multiple culture bottles at the same time, which improves work efficiency.

但是在组培实验中,需要每个培养单元的条件均统一,上述装置无法进行定量灌装,且控制不够方便。However, in the tissue culture experiment, the conditions of each culture unit need to be uniform, the above-mentioned device cannot perform quantitative filling, and the control is not convenient enough.

实用新型内容Utility model content

本实用新型旨在提供一种百合组培培养基灌装装置,以解决上述背景技术中的问题。The utility model aims to provide a lily tissue culture medium filling device to solve the above-mentioned problems in the background technology.

为实现上述目的,本实用新型采用如下技术方案:To achieve the above object, the utility model adopts the following technical solutions:

一种百合组培培养基灌装装置,包括培养基混合罐、位于培养基混合罐底部的支架,所述培养基混合罐顶面设有开口,且所述开口处设置有罐盖,所述培养基混合罐的内设置有搅拌装置,所述培养基混合罐的外壁设置有加热套,所述培养基混合罐的底部开设有出液口,所述出液口上设置有浮球阀,所述出液口与所述浮球阀的进水口连通,所述支架为u形结构,所述支架的两个侧壁之间水平设有u形槽,所述u形槽内设置有定量分装机构,所述支架的中部水平且位于所述出液口下方处设置有定量分装机构,所述定量分装机构包括若干互体积和形状均相同的定量槽,相邻的所述定量槽共用侧壁上均在同一高度处设置有开孔,所述浮球阀的出水口通过第一出液管与其中一个所述定量槽连通,所述第一出液管上设置有第一流量控制阀,所述浮球阀的浮球设置于另一个所述定量槽内,每个所述定量槽的底部均设置有第二出液管,且每个所述第二出液管的底端均穿过所述u形槽的底面,每个所述第二出液管上、且位于u 形槽下方处均设置有第二流量控制阀,所述第二出液管的下方对应设置有用于放置培养瓶的放置槽,且所述放置槽安装在所述支架上。A lily tissue culture medium filling device, comprising a medium mixing tank and a support located at the bottom of the medium mixing tank, the top surface of the medium mixing tank is provided with an opening, and the opening is provided with a tank cover, and the The medium mixing tank is provided with a stirring device, the outer wall of the medium mixing tank is provided with a heating jacket, the bottom of the medium mixing tank is provided with a liquid outlet, and the liquid outlet is provided with a floating ball valve. The liquid outlet is communicated with the water inlet of the floating ball valve, the bracket is a u-shaped structure, a u-shaped groove is horizontally arranged between two side walls of the bracket, and a quantitative dispensing mechanism is arranged in the u-shaped groove , the middle part of the support is horizontal and is located below the liquid outlet with a quantitative dispensing mechanism, the quantitative dispensing mechanism includes a number of quantitative grooves with the same mutual volume and shape, and the adjacent quantitative grooves share a side The walls are all provided with openings at the same height, the water outlet of the floating ball valve is communicated with one of the quantitative grooves through a first liquid outlet pipe, and the first liquid outlet pipe is provided with a first flow control valve, The floating ball of the floating ball valve is arranged in another quantitative tank, the bottom of each quantitative tank is provided with a second liquid outlet pipe, and the bottom end of each second liquid outlet pipe passes through On the bottom surface of the u-shaped groove, a second flow control valve is provided on each of the second liquid outlet pipes and below the u-shaped groove, and a corresponding valve for placing culture is provided below the second liquid outlet pipe. Placement slot for the bottle, and the placement slot is mounted on the bracket.

进一步的,所述搅拌装置包括螺旋叶片和旋转电机,所述旋转电机位于所述培养基混合罐顶部,且所述旋转电机的输出轴穿过所述培养基混合罐的顶部并固接有加长杆、且所述加长杆的底端连接有所述螺旋叶片。Further, the stirring device includes a helical blade and a rotary motor, the rotary motor is located at the top of the culture medium mixing tank, and the output shaft of the rotating motor passes through the top of the culture medium mixing tank and is fixed with a lengthening A rod, and the bottom end of the extension rod is connected with the helical blade.

进一步的,所述第一出液管的下管口高于所述开孔的高度。Further, the lower nozzle of the first liquid outlet pipe is higher than the height of the opening.

进一步的,所述定量分装机构外设置有保温棉。Further, thermal insulation cotton is provided outside the quantitative dispensing mechanism.

进一步的,所述第一流量控制阀和第二控流量制阀均为电磁阀,所述支架上还设置有控制器,所述第一流量控制阀、第二流量控制阀均与所述控制器电性连接,且每个第二流量控制阀均为串联。Further, the first flow control valve and the second flow control valve are both solenoid valves, the support is also provided with a controller, and the first flow control valve and the second flow control valve are both connected with the control valve. The devices are electrically connected, and each second flow control valve is connected in series.

与现有技术相比,本实用新型具有如下技术效果:Compared with the prior art, the utility model has the following technical effects:

1.通过设置加热套使培养基保持液体状态,通过搅拌装置使培养基保持均匀。1. The medium is kept in a liquid state by setting the heating mantle, and the medium is kept uniform by the stirring device.

2.通过设置定量分装机构,能够同时灌装多个培养基,且能够保证每个培养基的定量分装均匀,通过设置浮球阀,使其自动定量,操作便捷。2. By setting the quantitative dispensing mechanism, multiple media can be filled at the same time, and the quantitative dispensing of each medium can be ensured evenly. By setting the floating ball valve, it can be automatically quantitative and easy to operate.

附图说明Description of drawings

图1为本实用新型结构示意图。Figure 1 is a schematic structural diagram of the utility model.

图2为本实用新型的定量分装机构的结构示意图。FIG. 2 is a schematic structural diagram of the quantitative dispensing mechanism of the present invention.

图中:1-培养基混合罐,2-出液口,3-罐盖,4-加热套,5-浮球阀,6-支架, 7-定量槽,8-第二出液管,9-第一出液管,10-第二流量控制阀,11-放置槽,12- 旋转电机,13-螺旋叶片,14-第一流量控制阀,15-控制器。In the picture: 1- Medium mixing tank, 2- Liquid outlet, 3- Tank cover, 4- Heating jacket, 5- Floating ball valve, 6- Bracket, 7- Quantitative tank, 8- Second liquid outlet pipe, 9- The first liquid outlet pipe, 10-second flow control valve, 11-placement tank, 12-rotating motor, 13-spiral blade, 14-first flow control valve, 15-controller.

具体实施方式Detailed ways

下面结合附图,对本实用新型的一个具体实施方案进行详细描述,但应当理解,本实用新型的保护范围并不受具体实施方式的限制。A specific embodiment of the present invention will be described in detail below with reference to the accompanying drawings, but it should be understood that the protection scope of the present invention is not limited by the specific embodiment.

实施例1Example 1

参见附图1-2,一种百合组培培养基灌装装置,包括培养基混合罐1、位于培养基混合罐1底部的支架6,培养基混合罐1顶部具有开口,且开口处铰接有罐盖3,培养基混合罐1的内设置有搅拌装置,搅拌装置包括旋转电机12 和螺旋叶片13,旋转电机12位于培养基混合罐1上顶面,且旋转电机12的输出轴穿过培养基混合罐1的顶面并固接有加长杆、且加长杆底端螺接有螺旋叶片13,方便将培养基搅拌均匀;Referring to the accompanying drawings 1-2, a lily tissue culture medium filling device, comprising a medium mixing tank 1, a bracket 6 located at the bottom of the medium mixing tank 1, the top of the medium mixing tank 1 has an opening, and the opening is hinged with The tank cover 3, the medium mixing tank 1 is provided with a stirring device, the stirring device includes a rotating motor 12 and a spiral blade 13, the rotating motor 12 is located on the top surface of the medium mixing tank 1, and the output shaft of the rotating motor 12 passes through the culture medium. The top surface of the base mixing tank 1 is fixed with an extension rod, and the bottom end of the extension rod is screwed with a spiral blade 13, which is convenient for stirring the culture medium evenly;

培养基混合罐1的外壁设置有加热套4,本实施例的加热套4为新瑞琪电热设备有限公司的柔性电加热套,具有升温快、保温好、发热均匀、热效率高等,温控系统具有智能PID控制,精确控温,数显工作温度,培养基混合罐1 的底部开设有出液口2,可以使培养基通过自身重力流出,出液口2上设置有浮球阀5,本实施例的浮球阀5为维成DN8F浮动球球阀,包括进水口、出水口、浮球、连杆、支架和球阀,出液口2与浮球阀5的进水口连通,培养基混合罐1的底部还设置有支架6,支架4为u形,支架的两个侧壁之间水平设有 u形槽,u形槽内设置有定量分装机构,设置支架6的作用是使培养基混合罐 1腾空,便于出液,也是为了能够在底部设置定量分装机构。The outer wall of the culture medium mixing tank 1 is provided with a heating jacket 4. The heating jacket 4 in this embodiment is a flexible electric heating jacket from Xinruiqi Electric Heating Equipment Co., Ltd., which has the advantages of fast heating, good heat preservation, uniform heating, high thermal efficiency, and a temperature control system. It has intelligent PID control, precise temperature control, and digital display of working temperature. The bottom of the medium mixing tank 1 is provided with a liquid outlet 2, which can make the medium flow out by its own gravity. The liquid outlet 2 is provided with a floating ball valve 5. This implementation The floating ball valve 5 of the example is a Weicheng DN8F floating ball valve, including a water inlet, a water outlet, a floating ball, a connecting rod, a bracket and a ball valve, the liquid outlet 2 is communicated with the water inlet of the floating ball valve 5, and the bottom of the medium mixing tank 1 is connected. A support 6 is also provided, the support 4 is u-shaped, a u-shaped groove is horizontally arranged between the two side walls of the support, and a quantitative sub-packaging mechanism is arranged in the u-shaped groove, and the function of setting the support 6 is to make the culture medium mixing tank 1 It is vacated to facilitate liquid discharge, and it is also to be able to set a quantitative dispensing mechanism at the bottom.

支架6的中部且位于出液口2下方处水平设置有定量分装机构,定量分装机构包括若干体积和形状均相同的定量槽7,本实施例中,定量槽7的数量为 10个,如图2所示,相邻的定量槽7共用的侧壁上均在同一高度处设置有开孔,浮球阀5的出液口通过第一出液管9与其中一个定量槽7连通,第一出液管9 上设置有第一流量控制阀,浮球阀5的浮球设置于另一个定量槽7,每个定量槽7的底部均连通设置有第二出液管8,且每个第二出液管8的底端均穿过u 形槽的底面,每个第二出液管8上、且位于所述u形槽下方处均设置有第二流量控制阀10,第二出液管8的下方对应设置有用于放置培养瓶的放置槽11,且放置槽11安装在支架6上。The middle part of the support 6 and the lower part of the liquid outlet 2 are horizontally provided with a quantitative dispensing mechanism. The quantitative dispensing mechanism includes a number of quantitative grooves 7 with the same volume and shape. In this embodiment, the number of quantitative grooves 7 is 10, As shown in FIG. 2 , the side walls shared by the adjacent quantitative grooves 7 are all provided with openings at the same height, and the liquid outlet of the float valve 5 is communicated with one of the quantitative grooves 7 through the first liquid outlet pipe 9. A liquid outlet pipe 9 is provided with a first flow control valve, the floating ball of the floating ball valve 5 is arranged in another quantitative tank 7, the bottom of each quantitative tank 7 is connected with a second liquid outlet pipe 8, and each The bottom ends of the two liquid outlet pipes 8 pass through the bottom surface of the u-shaped groove, and each second liquid outlet pipe 8 is provided with a second flow control valve 10 on the bottom of the u-shaped groove. A placing groove 11 for placing a culture flask is correspondingly provided below the tube 8 , and the placing groove 11 is installed on the bracket 6 .

本实施例中,第一出液管9的下管口高于开孔的高度,防止第一出液管9 浸入液面,使定量槽7中培养基的体积虚高;定量分装机构外设置有保温棉,防止培养基凝固。In this embodiment, the lower orifice of the first liquid outlet pipe 9 is higher than the height of the opening to prevent the first liquid outlet pipe 9 from being immersed in the liquid surface, so that the volume of the culture medium in the quantitative tank 7 is falsely high; A thermal insulation cotton is provided to prevent the medium from coagulating.

为了控制方便,本实施例中,第一流量控制阀14和第二流量控制阀10均为电磁阀,支架6上还设置有控制器15,控制器型号为STC859C52,第一流量控制阀14、第二流量控制阀10均与控制器15电性连接,且每个第二流量控制阀10均为串联,控制器上设置有用于控制第一流量控制阀14的第一开关和控制第二流量控制阀10的第二开关For the convenience of control, in this embodiment, the first flow control valve 14 and the second flow control valve 10 are both solenoid valves, and the support 6 is also provided with a controller 15, the controller model is STC859C52, the first flow control valve 14, The second flow control valves 10 are all electrically connected to the controller 15, and each second flow control valve 10 is connected in series. The controller is provided with a first switch for controlling the first flow control valve 14 and a second flow control valve. Second switch of control valve 10

本实施例的一种百合培养基灌装装置的工作原理:The working principle of a lily culture medium filling device of the present embodiment:

打开罐盖3,向培养基混合罐中加入待分装的培养基,打开加热套4和旋转电机12,使培养基保持一定的温度和保持均匀,通过第一开关打开第一流量控制阀14,使培养基通过第一出液管进入一个定量槽7,当该定量槽7中的培养基液面高度升高至开孔的高度时,培养基通过开孔流向其它的定量槽7,直至每个定量槽7中的液面高度均相等,且略高于开孔的高度(通过预先设置浮球阀5的位置,使浮球到达该高度时,浮球阀5关闭),此时浮球随着液面上升,自动将浮球阀5关闭,然后通过第一开关关闭第一流量控制阀14,通过第二开关开启第二流量控制阀10,向培养瓶中注入培养基,达到定量灌装的目的,灌装完毕,关闭第二流量控制阀10,打开第一流量控制阀14向定量槽7中注培养基,进行下一批次的操作。Open the tank cover 3, add the medium to be dispensed into the medium mixing tank, turn on the heating jacket 4 and the rotary motor 12, keep the medium at a certain temperature and keep it uniform, and open the first flow control valve 14 through the first switch , the medium enters a quantitative tank 7 through the first liquid outlet pipe, when the liquid level of the medium in the quantitative tank 7 rises to the height of the opening, the medium flows through the opening to other quantitative tanks 7 until The height of the liquid level in each quantitative tank 7 is equal and slightly higher than the height of the opening (by setting the position of the float valve 5 in advance, when the float ball reaches this height, the float valve 5 is closed). When the liquid level rises, the float valve 5 is automatically closed, then the first flow control valve 14 is closed through the first switch, the second flow control valve 10 is opened through the second switch, and culture medium is injected into the culture bottle to achieve quantitative filling. For the purpose of filling, close the second flow control valve 10 and open the first flow control valve 14 to inject culture medium into the quantitative tank 7 to perform the next batch of operations.

以上的仅为本实用新型的一个实施例,但是本实用新型实施例并非仅限于此,任何本领域能思之的变化都应落在本实用新型的保护范围之内。The above is only an embodiment of the present invention, but the embodiment of the present invention is not limited to this, and any changes that can be conceived in the field should fall within the protection scope of the present invention.

Claims (5)

1.一种百合组培培养基灌装装置,包括培养基混合罐(1)、位于培养基混合罐(1)底部的支架(6),其特征在于,所述培养基混合罐(1)顶面设有开口,所述开口处设置有罐盖(3),所述培养基混合罐(1)内设置有搅拌装置,所述培养基混合罐(1)的外壁设置有加热套(4),所述培养基混合罐(1)的底部开设有出液口(2),所述出液口(2)处设置有浮球阀(5),所述出液口(2)与所述浮球阀(5)的进水口连通;所述支架(6)为u形结构,所述支架(6)的两个侧壁之间通过连接杆水平连接有u形槽,所述u形槽内设置有定量分装机构,所述定量分装机构包括若干体积和形状均相同的定量槽(7),相邻的所述定量槽(7)共用的侧壁上均在同一高度处设置有开孔,所述浮球阀(5)的出水口通过第一出液管(9)与其中一个所述定量槽(7)连通,所述第一出液管(9)上设置有第一流量控制阀(14),所述浮球阀(5)的浮球设置于另一个所述定量槽(7)内,每个所述定量槽(7)的底部均连通设置有第二出液管(8),且每个所述第二出液管(8)的底端均穿过所述u形槽的底面,每个所述第二出液管(8)上、且位于所述u形槽下方处均设置有第二流量控制阀(10),每个所述第二出液管(8)的下方均对应设置有用于放置培养瓶的放置槽(11),且所述放置槽(11)设置在所述支架(6)底板上。1. a lily tissue culture medium filling device, comprising a medium mixing tank (1), a support (6) positioned at the bottom of the medium mixing tank (1), characterized in that the medium mixing tank (1) The top surface is provided with an opening, the opening is provided with a tank cover (3), a stirring device is provided in the medium mixing tank (1), and a heating jacket (4) is provided on the outer wall of the medium mixing tank (1). ), the bottom of the culture medium mixing tank (1) is provided with a liquid outlet (2), the liquid outlet (2) is provided with a floating ball valve (5), and the liquid outlet (2) and the The water inlet of the floating ball valve (5) is communicated; the bracket (6) is a u-shaped structure, and a u-shaped groove is horizontally connected between two side walls of the bracket (6) through a connecting rod. A quantitative dispensing mechanism is provided, and the quantitative dispensing mechanism includes several quantitative grooves (7) with the same volume and shape, and the shared side walls of the adjacent quantitative grooves (7) are provided with openings at the same height. The water outlet of the float valve (5) is communicated with one of the quantitative grooves (7) through the first liquid outlet pipe (9), and the first liquid outlet pipe (9) is provided with a first flow control A valve (14), the floating ball of the floating ball valve (5) is arranged in another said quantitative groove (7), and the bottom of each said quantitative groove (7) is connected with a second liquid outlet pipe (8). ), and the bottom end of each second liquid outlet pipe (8) passes through the bottom surface of the u-shaped groove, and each second liquid outlet pipe (8) is located on the u-shaped groove. A second flow control valve (10) is provided at the lower part, and a placing groove (11) for placing a culture bottle is correspondingly provided below each of the second liquid outlet pipes (8), and the placing groove (11) ) is arranged on the bottom plate of the bracket (6). 2.根据权利要求1所述的一种百合组培培养基灌装装置,其特征在于,所述搅拌装置包括螺旋叶片(13)和旋转电机(12),所述旋转电机(12)位于所述培养基混合罐(1)顶部,且所述旋转电机(12)的输出轴穿过所述培养基混合罐(1)的顶部并固接有加长杆、且所述加长杆的底端连接有所述螺旋叶片(13)。2. a kind of lily tissue culture medium filling device according to claim 1, is characterized in that, described stirring device comprises helical blade (13) and rotary motor (12), and described rotary motor (12) is located in the The top of the medium mixing tank (1), and the output shaft of the rotating motor (12) passes through the top of the medium mixing tank (1) and is fixed with an extension rod, and the bottom end of the extension rod is connected There are said helical blades (13). 3.根据权利要求1所述的一种百合组培培养基灌装装置,其特征在于,所述第一出液管(9)的下管口高于所述开孔的高度。3 . The lily tissue culture medium filling device according to claim 1 , wherein the lower orifice of the first liquid outlet pipe (9) is higher than the height of the opening. 4 . 4.根据权利要求1所述的一种百合组培培养基灌装装置,其特征在于,所述定量分装机构外部包裹有保温棉。4 . The lily tissue culture medium filling device according to claim 1 , wherein the quantitative dispensing mechanism is externally wrapped with thermal insulation cotton. 5 . 5.根据权利要求1所述的一种百合组培培养基灌装装置,其特征在于,所述第一流量控制阀(14)和第二流量控制阀(10)均为电磁阀,所述支架(6)上还设置有控制器(15),所述第一流量控制阀(14)、第二流量控制阀(10)均与所述控制器(15)电性连接,且每个第二流量控制阀(10)均为串联。5. a kind of lily tissue culture medium filling device according to claim 1, is characterized in that, described first flow control valve (14) and second flow control valve (10) are electromagnetic valve, described A controller (15) is also provided on the bracket (6), the first flow control valve (14) and the second flow control valve (10) are electrically connected to the controller (15), and each of the first flow control valve (14) and the second flow control valve (10) is electrically connected to the controller (15). Both flow control valves (10) are connected in series.
CN201920884860.4U 2019-06-12 2019-06-12 Lily tissue culture medium filling device Expired - Fee Related CN210143514U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112352676A (en) * 2020-10-28 2021-02-12 广西壮族自治区农业科学院 Multipoint automatic quantitative tissue culture solution stirring and filling machine
CN115281041A (en) * 2022-07-05 2022-11-04 吉林省农业科学院 Morchella culture device and using method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112352676A (en) * 2020-10-28 2021-02-12 广西壮族自治区农业科学院 Multipoint automatic quantitative tissue culture solution stirring and filling machine
CN115281041A (en) * 2022-07-05 2022-11-04 吉林省农业科学院 Morchella culture device and using method thereof

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