CN203033993U - Fermentation device for acidophilic bacterium culture - Google Patents
Fermentation device for acidophilic bacterium culture Download PDFInfo
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- CN203033993U CN203033993U CN 201220641871 CN201220641871U CN203033993U CN 203033993 U CN203033993 U CN 203033993U CN 201220641871 CN201220641871 CN 201220641871 CN 201220641871 U CN201220641871 U CN 201220641871U CN 203033993 U CN203033993 U CN 203033993U
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- fermentor tank
- fermentation
- return line
- whipping appts
- liquid
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Abstract
The utility model discloses a fermentation device for acidophilic bacterium culture, which mainly comprises a stirring device, a transmission device, a suction filtration device, a sealing cover, a sediment hopper, a bracket, a technological connection pipe and accessories. Particularly, a fermentation tank consists of a top cover and a tank body; the stirring device consists of a stirrer and a rod; the transmission device is adopted for driving the stirring device, and mainly comprises a motor, a speed reducer, a coupler and a transmission shaft; and a device with various functions, a support, the technological connection pipe and other accessories form the integral fermentation tank. The fermentation device has the benefits that the fermentation device is high in corrosion resistance, is completely suitable for large-scale culture of acidophilic bacteria, and can replace a commonly-used fermentation tank in the market, and the product cost is suitable.
Description
Technical field
The utility model relates to microbial fermentation and cultivates the field, specifically, specially refers to a kind of fermentation unit for the acidophilic bacteria cultivation.
Background technology
The acidophilic bacteria commonly used in the biological metallurgy industry mainly comprises thiobacillus ferrooxidant (Acidthiobacillus ferrooxidans), thiobacillus thiooxidans (Acidthiobacillus thiooxidans), the little spirillum of iron protoxide (Leptospirillum ferrooxidans) etc., these bacterial classifications at ore smelting, remove in the coal industry such as sulphur and carry out industrial production.At present, both at home and abroad attempt adopting these bacterial classifications to play a role at aspects such as electron wastes processing, waste harmless treatment, contaminated soil improvement in the big quantity research, need cultivate a large amount of bacterial classifications in advance using aspect these Pollution abatements.And the common working condition of these acidophilic bacteria kinds is relatively harsher, as medium pH value be low to moderate about 1.0, characteristics such as oxidisability is stronger, and employed part nutritive substance can not pass through heat sterilization in these spawn culture processes, and commercial fermentor tank all is based on metal material and heating high-pressure sterilization usually on the market, is not suitable for being applied to the cultivation of acidophilic bacteria.
In sum, at the deficiencies in the prior art, need design especially and develop a kind of fermentation unit that is applicable to the acidophilic bacteria large scale culturing.
The utility model content
The purpose of this utility model is to provide a overall plastic fermentor tank that is fit to tolerate highly corrosive, need not heat and can reach sterilising effect, has overcome the deficiency in the conventional art, thereby realizes the purpose of this utility model.
The technical problem that the utility model solves can realize by the following technical solutions:
A kind of fermentation unit for the acidophilic bacteria cultivation, it comprises
One fermentor tank, the top of described fermentor tank is provided with detachable sealing cover, and the bottom of fermentor tank is provided with the precipitation funnel and is used for collecting and discharging the solid matter that culturing process produces;
One whipping appts, described whipping appts comprises stir shaft and agitating vane, is used for the nutrient solution in the stirred fermentor, and described stir shaft is arranged at the inside of fermentor tank, and its surface is provided with agitating vane;
One transmission mechanism, described transmission mechanism comprises motor, step-down gear and shaft coupling, and an end of described step-down gear connects motor, and the other end of described step-down gear connects the stir shaft of whipping appts by shaft coupling;
One feeding device, described feeding device comprise feed hopper, filter membrane, suction filtration device and feed-pipe, and the outlet material end of described feed hopper connects filter membrane and suction filtration device successively, and the discharge port end of described suction filtration device connects feed-pipe;
One heating unit, described heating unit is installed on the inside of fermentor tank, is used for little heating of fermentation pot liquid, and the Heating temperature scope is 25-35 ℃;
One reflux, described reflux comprises return line, reflux pump, stopping valve and water shoot, the two ends of described return line are communicated with the top and bottom of fermentor tank respectively, the bottom of return line is provided with reflux pump, stopping valve also is installed on the described return line, and the return line of stopping valve below is communicated with water shoot.
In an embodiment of the present utility model, the gap location of the stir shaft of described step-down gear and whipping appts is provided with gland seal device, and the junction of described whipping appts and fermentor tank is provided with the flange of sealing usefulness.
In an embodiment of the present utility model, a side of described fermentor tank is provided with the liquid senior executive who links to each other with fermentor tank, is used for observing the liquid level that feeds solution in the fermentor tank and the variation of fermenting back bacterium liquid.
In an embodiment of the present utility model, described fermentor tank is provided with the pH meter that links to each other with fermentor tank, is used for the variation of monitoring culturing process bacterium liquid pH value.
In an embodiment of the present utility model, described precipitation funnel reflector for the impact of buffering liquid is installed, the precipitation funnel exit end is communicated with and clears washing lotion and manage admittedly.
The beneficial effects of the utility model are: the tolerance highly corrosive, and suitable fully concerning the large scale culturing of acidophilic bacteria, can replace fermentor tank commonly used on the market, and product price is also suitable.
Description of drawings
Fig. 1 is the structural representation of the fermentation unit of cultivating for acidophilic bacteria described in the utility model.
Embodiment
For technique means, creation characteristic that the utility model is realized, reach purpose and effect is easy to understand, below in conjunction with embodiment, further set forth the utility model.
As shown in Figure 1, a kind of fermentation unit for the acidophilic bacteria cultivation described in the utility model, adopt the mechanical agitation type structure, utilize the effect of mechanical stirrer, air and the nutrient solution crossed through 22 μ m aperture micro-filtrate membrane filtrations are fully mixed, to guarantee the required sterile air of microorganism growth breeding; All nutrient solutions all are to enter fermentor tank after the filtering with microporous membrane degerming; Fermentor tank charges into ozone when using first, sterilization.
It mainly comprises:
Fermentor tank 1, the tank body of fermentor tank 1 is that PP or PVC rigid plastics are cylindric, the top is detachable sealing cover 2, the bottom is that precipitation funnel 16 is used for collecting and discharging the issuable small amount of solid state material of acidophilic bacteria culturing process, described precipitation funnel 16 is equipped with the reflector 18 for the impact of buffering liquid, and the exit end of precipitation funnel 16 is communicated with and clears washing lotion and manage 17 admittedly.A plurality of apertures are arranged on the tank body, be respectively opening for feed, pH electrode mouth, heating rod device, the mouth of pipe that refluxes, look the mouth of pipe, clear washing lotion Gu Kou.
Whipping appts, described whipping appts comprise stir shaft 5 and agitating vane 23, are used for the nutrient solution in the stirred fermentor 1, and described stir shaft 4 is arranged at the inside of fermentor tank 1, and its surface is provided with agitating vane 23.
Transmission mechanism, be used to whipping appts that power resources are provided, described transmission mechanism comprises motor 9, step-down gear 8 and shaft coupling 7, wherein motor 9, step-down gear 8 are installed in the top of fermentor tank 1 by extra support 6, one end of described step-down gear 8 connects motor 9, and the other end of described step-down gear 8 connects the stir shaft 5 of whipping appts by shaft coupling 7.
Feeding device, described feeding device comprises feed hopper 10, filter membrane 11, suction filtration device 12 and feed-pipe 13, described feed hopper 10 is used for feeding nutrient solution and bacterium liquid, its outlet material end is connected with in turn for filtration method and removes the filter membrane 11 of nutrient solution and air microorganism and feed fermentor tank fast for nutrient solution behind membrane filtration, and the suction filtration device 12 of residual ozone in when ozone sterilization finishes, removing jar, the discharge port end of described suction filtration device 12 connects feed-pipe 13.
Heating unit 24, described heating unit 24 is installed on the inside of fermentor tank 1, is used for little heating of liquid in the fermentor tank 1, and the Heating temperature scope is 25-35 ℃.
Reflux, described reflux comprises return line 22, reflux pump 19, stopping valve 21 and water shoot 20, the two ends of described return line 22 are communicated with the top and bottom of fermentor tank 1 respectively, are used for the liquid output of the circulation of solution and fermentation back bacterium liquid between liquid level up and down in the fermentor tank 1.The bottom of return line 22 is provided with reflux pump 19, described reflux pump 19 be used to liquid in the fermentor tank 1 up and down between liquid level the circulation of solution and fermentation back bacterium liquid power output is provided.On the described return line 22 stopping valve 21 is installed also, the return line of stopping valve 21 belows is communicated with water shoot 20.
Some other annex comprises:
Gland seal device 4 and flange 3, described flange is arranged at the gap location of the stir shaft 5 of step-down gear 8 and whipping appts, and described flange 3 is arranged at the junction of whipping appts and fermentor tank 1, and flange 3 can adopt rubber ring, be used for the linking of whipping appts and gland seal device 4, guarantee sealing.
Liquid senior executive 14, described liquid senior executive 14 is arranged at a side of fermentor tank, links to each other with fermentor tank 1, is used for observing the liquid level that feeds solution in the fermentor tank 1 and the variation of fermenting back bacterium liquid.
More than show and described ultimate principle of the present utility model and principal character and advantage of the present utility model.The technician of the industry should understand; the utility model is not restricted to the described embodiments; that describes in above-described embodiment and the specification sheets just illustrates principle of the present utility model; under the prerequisite that does not break away from the utility model spirit and scope; the utility model also has various changes and modifications, and these changes and improvements all fall in claimed the utility model scope.The claimed scope of the utility model is defined by appending claims and equivalent thereof.
Claims (5)
1. one kind is used for the fermentation unit that acidophilic bacteria is cultivated, and it is characterized in that it comprises
One fermentor tank, the top of described fermentor tank is provided with detachable sealing cover, and the bottom of fermentor tank is provided with the precipitation funnel and is used for collecting and discharging the solid matter that culturing process produces;
One whipping appts, described whipping appts comprises stir shaft and agitating vane, is used for the nutrient solution in the stirred fermentor, and described stir shaft is arranged at the inside of fermentor tank, and its surface is provided with agitating vane;
One transmission mechanism, described transmission mechanism comprises motor, step-down gear and shaft coupling, and an end of described step-down gear connects motor, and the other end of described step-down gear connects the stir shaft of whipping appts by shaft coupling;
One feeding device, described feeding device comprise feed hopper, filter membrane, suction filtration device and feed-pipe, and the outlet material end of described feed hopper connects filter membrane and suction filtration device successively, and the discharge port end of described suction filtration device connects feed-pipe;
One heating unit, described heating unit is installed on the inside of fermentor tank, is used for little heating of fermentation pot liquid, and the Heating temperature scope is 25-35 ℃;
One reflux, described reflux comprises return line, reflux pump, stopping valve and water shoot, the two ends of described return line are communicated with the top and bottom of fermentor tank respectively, the bottom of return line is provided with reflux pump, stopping valve also is installed on the described return line, and the return line of stopping valve below is communicated with water shoot.
2. a kind of fermentation unit of cultivating for acidophilic bacteria according to claim 1, it is characterized in that, the gap location of the stir shaft of described step-down gear and whipping appts is provided with gland seal device, and the junction of described whipping appts and fermentor tank is provided with the flange of sealing usefulness.
3. a kind of fermentation unit for the acidophilic bacteria cultivation according to claim 1 is characterized in that a side of described fermentor tank is provided with the liquid senior executive who links to each other with fermentor tank, is used for observing the liquid level that feeds solution in the fermentor tank and the variation of fermenting back bacterium liquid.
4. a kind of fermentation unit for the acidophilic bacteria cultivation according to claim 1 is characterized in that described fermentor tank is provided with the pH meter that links to each other with fermentor tank, is used for the variation of monitoring culturing process bacterium liquid pH value.
5. a kind of fermentation unit of cultivating for acidophilic bacteria according to claim 1 is characterized in that, described precipitation funnel reflector for the impact of buffering liquid is installed, the precipitation funnel exit end is communicated with and clears washing lotion and manage admittedly.
Priority Applications (1)
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CN 201220641871 CN203033993U (en) | 2012-11-28 | 2012-11-28 | Fermentation device for acidophilic bacterium culture |
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CN 201220641871 CN203033993U (en) | 2012-11-28 | 2012-11-28 | Fermentation device for acidophilic bacterium culture |
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CN 201220641871 Expired - Fee Related CN203033993U (en) | 2012-11-28 | 2012-11-28 | Fermentation device for acidophilic bacterium culture |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104328280A (en) * | 2014-10-15 | 2015-02-04 | 上海第二工业大学 | Method and equipment for recycling all metal components in waste flexible circuit board |
CN106801004A (en) * | 2015-11-25 | 2017-06-06 | 湖南恒光化工有限公司 | A kind of fermentation tank |
CN111676136A (en) * | 2020-06-23 | 2020-09-18 | 肇庆大华农生物药品有限公司 | Bioreactor for improving antigen yield |
-
2012
- 2012-11-28 CN CN 201220641871 patent/CN203033993U/en not_active Expired - Fee Related
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104328280A (en) * | 2014-10-15 | 2015-02-04 | 上海第二工业大学 | Method and equipment for recycling all metal components in waste flexible circuit board |
CN106801004A (en) * | 2015-11-25 | 2017-06-06 | 湖南恒光化工有限公司 | A kind of fermentation tank |
CN111676136A (en) * | 2020-06-23 | 2020-09-18 | 肇庆大华农生物药品有限公司 | Bioreactor for improving antigen yield |
CN111676136B (en) * | 2020-06-23 | 2023-08-25 | 肇庆大华农生物药品有限公司 | Bioreactor for improving antigen yield |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C17 | Cessation of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130703 Termination date: 20131128 |