CN207525252U - Liquid system is changed suitable for what spatial cell was cultivated automatically - Google Patents
Liquid system is changed suitable for what spatial cell was cultivated automatically Download PDFInfo
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- CN207525252U CN207525252U CN201721316677.1U CN201721316677U CN207525252U CN 207525252 U CN207525252 U CN 207525252U CN 201721316677 U CN201721316677 U CN 201721316677U CN 207525252 U CN207525252 U CN 207525252U
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Abstract
Liquid system is changed suitable for what spatial cell was cultivated automatically this patent discloses a kind of, including：Nutrient solution bag, silicone rubber tube, culture unit, peristaltic pump, waste fluid bag.Nutrient solution bag is soft nutrient solution liquid storing bag, and fresh nutrient solution is stored for low temperature, and only there are one liquid in-out mouths on liquid storing bag, can install the straight-through pipe connecting piece of connection silicone rubber tube thereon.Silicone rubber tube connects Main functional units, both can be used for that cellular liquid transports and preheating flows into nutrient solution, can also discharge nutrient solution.Cell culture unit can fully be spread for cell culture and observation automatically, fresh nutrient solution in culture unit.Waste fluid bag is soft liquid storing bag, for storing the waste liquid of culture unit outflow.The control system of small peristaltic pump and pump is used to generate power to liquid transport.This patent is easy for installation, temperature equalization, air-tightness are good, convenient for observation, realizes nutrient solution of the spatial cell automatically needed for culture and changes gear demand, feasible through ground and space flight test verification.
Description
Technical field
This patent is related to Biotechnology In Space, particularly relates to a kind of be suitable under space microgravity environment, applied to space
What cells in vitro was cultivated automatically changes liquid system.
Background technology
Cell culture invitro technology has become the basis of the relevant biological study of cell, as space life science is ground
The development studied carefully, people by cell, tissue culture technique with microgravity environment be combined produce one of Tissue Engineering Study it is new
Field --- microgravity organizational project.Cell culture and tissue construction under simulation space microgravity condition research shows that, microgravity
Environmental benefits are formed and have functional tissue-like architecture in the three dimensional growth of cell, and no matter the three-dimensional tissue after culture is from form
All closer to normal body tissue in upper or gene expression.This microgravity will likely be to the action effect of cell
Future organization engineering and regenerative medicine research provide a new way.Cell culture invitro be it is a kind of provided for cell growth it is suitable
Suitable environment and the modern biology experimental technique of nutrition supply.Cell is very sensitive, growing environment is required very severe
It carves.Under normal circumstances, the required environment of cell must be kept constant, including：Suitable temperature, humidity, acid-base value (pH
Value), specific gaseous environment and will keep sterile, nontoxic environment etc. at certain nutriment.At present, cell culture
Laboratory routine operation includes, and culture dish periodically is replaced fresh nutrient solution for cell in aseptic operating platform by testing crew,
Then culture dish is placed in constant incubator and cultivated.Mass cell culture is carried out in cell factory then needs complexity
Heat preservation and liquid management system, it is impossible to meet the design condition of spatial cell culture.
Spatial cell is cultivated automatically to be needed, in microgravity, enough nutrition, microgravity feelings to be provided for cell growth
Under condition, liquid will not sink to culture unit bottom, it is therefore desirable to which entire hydraulic seal is being changed liquid internal system；Cell culture needs
It to carry out in an aseptic environment, therefore design needs to facilitate system assembles；In order to reduce the influence of ground gravity benefit, shorten thin
The shorter incubation time of the born of the same parents under gravitational conditions, the interval time between recovery cell and rocket launching the better, it is therefore desirable to grasp
Making process need to be simple and reliable.In addition, spaceflight apparatus requirement miniaturization, automation, can run under the conditions of nobody for a long time.Therefore it sets
Meter disclosure satisfy that miniaturization of the cell spaces automatically needed for culture, ease of assembly, reliability it is high change liquid system just into solution
The critical issue of spatial cell culture technique.
Invention content
The purpose of this patent be to provide it is a kind of suitable for spatial cell cultivate automatically change liquid system, including nutrient solution bag,
Silicone rubber tube, culture unit, peristaltic pump, waste fluid bag.
A kind of to change liquid system suitable for what spatial cell was cultivated automatically, including nutrient solution bag 1, silicone rubber tube 2 cultivates unit
3, peristaltic pump 4, waste fluid bag 5.
Nutrient solution bag 1 uses the soft liquid storing bag of flexibility of the Kynoar PVDF materials of good biocompatibility.
The processing of 3 both ends of unit is cultivated there are one inlet and a liquid outlet, as shown in Fig. 2, including：Reactor cap 6；It is close
Seal 7；Reactor body 8；Pipe fitting 9.
The peristaltic pump 4 is the easy-to-dismount peristaltic pump of pump head, facilitates pipeline dismounting during sterile working.
The waste fluid bag 5, using the soft liquid storing bag of flexibility of Kynoar PVDF materials..
Only there are one liquid in-out mouth in nutrient solution bag 1, which is connected by straight-through pipe connecting piece with silicone rubber tube 2
It connects；Nutrient solution bag 1 is positioned in fixed low temperature box, and box volume fills the volume after liquid more than or equal to nutrient solution bag.Training
The inlet for supporting unit 3 is connected by silicone rubber tube 2 with nutrient solution bag 1, liquid outlet by silicone rubber tube 2 through peristaltic pump 4 and
Waste fluid bag 5 is connected；Culture unit upper cover can be opened, and be sealed after being packed into cell.
Realize this patent technical solution be：
(1) face when penetrating installation, by after sterilizing nutrient solution bag, silicone rubber tube, culture unit, peristaltic pump, waste fluid bag is in nothing
Bacterium operation console opens packaging；
(2) fresh medium is poured into nutrient solution bag, pour into needed for after amount of liquid, gas in bag is emptied, then with stopping
Blood clamps the separate liquid bag end for clamping silicone rubber tube；
(3) cell is packed into culture unit；
(4) silicone rubber tube is connected, it is ensured that without bubble in nutrient solution bag --- silicone rubber tube --- culture unit；
(5) unit to the silicone rubber tube between waste fluid bag will be cultivated and is pressed into Peristaltic pump head；
(6) finally connection empties the empty waste fluid bag of gas, completes assembling.
When in-orbit progress cell is cultivated automatically, control system opens peristaltic pump according to experiment flow, and peristaltic pump will be cultivated single
Liquid in member is pumped into waste fluid bag, and due to pressure, the nutrient solution in nutrient solution bag flows into culture unit, realizes to cell
The automatic replacement of nutrient solution.Wriggling flow rate pump is meeting liquid always in the case of replacement amount, on the one hand flow velocity will be as possible slowly
Guarantee nutrient solution flow into cultivate unit before temperature be preheated to cultivation temperature, on the other hand in order to ensure fresh nutrition
Liquid has the sufficient time to be diffused in culture unit.
The advantages of this patent：
1st, the system is easy for installation, is connected in series nutrient solution bag, culture unit and waste fluid bag by silicone rubber tube, wriggles
Pump uses the peristaltic pump of pump head easy to install；
2nd, the solution temperature of the system is uniform, when changing liquid, slow feed liquor, it can be ensured that flow into the nutrient solution in culture unit
Temperature has reached cultivation temperature.
3rd, the entire cultivating system of the system is gas-tight design, has not only met the needs of space culture, but also reduces reaction
The contaminated probability of system.
4th, peristaltic pump is placed between culture unit and waste fluid bag by the liquid system of changing of the system, is produced during wriggling pump work
Raw bubble will not enter culture unit, ensure that cell culture and observation condition.
Description of the drawings
For spatial cell, liquid system structure diagram is changed in culture to Fig. 1 automatically.
In figure：1. nutrient solution bag；2. silicone rubber tube；3. cultivate unit；4. peristaltic pump；5. waste fluid bag.
Fig. 2 is culture cellular construction schematic diagram.
In figure：6. reactor cap；7. sealing ring；8. reactor；9. pipe fitting.
Specific embodiment
This patent described device is verified that concrete operation step is such as in space flight test and ground Comparability test
Under：
(1) each group parts after sterilizing are assembled in aseptic operating platform, including filling sterile into nutrient solution bag
Nutrient solution connects silicone rubber tube, opens culture unit upper cover, moves into cell, tighten upper cover, exhausts in culture unit and pipeline
Gas connects peristaltic pump, connects waste fluid bag, completes assembling.
(2) assembled pipe-line system is packed into cell incubator, including nutrient solution bag is put into low-temperature conservation box, will trained
It supports unit to be fixed on 37 DEG C of culture plates, fixes peristaltic pump and silicone rubber tube.
(3) start to test according to experiment process, including carrying out 37 DEG C of temperature controls to culture unit, nutrient solution bag is stored
Area freezes, and is allowed to, less than 10 DEG C, open a peristaltic pump daily, each opening time continues ten minutes, replaces nutrient solution
Measure about 2.5ml.
(4) during testing, the cellular morphology in culture unit can be observed automatically by microscope, it after the test, will
Pipe-line system is taken out from incubator, and is disassembled in aseptic operating platform.
Show that liquid system is changed in culture to this jacket cell automatically, can meet phase of cell growth through ground experiment and space test
Between the demand replaced automatically of nutrient solution.
Claims (1)
1. a kind of change liquid system suitable for what spatial cell was cultivated automatically, including nutrient solution bag (1), silicone rubber tube (2), culture is single
First (3), peristaltic pump (4), waste fluid bag (5), it is characterised in that：
The nutrient solution bag (1) using the Kynoar PVDF materials of good biocompatibility the soft liquid storing bag of flexibility；
Culture unit (3) both ends processing is there are one inlet and a liquid outlet, including reactor cap (6)；Sealing ring
(7)；Reactor body (8)；Pipe fitting (9)；
The peristaltic pump (4) is the easy-to-dismount peristaltic pump of pump head, facilitates pipeline dismounting during sterile working；
The waste fluid bag (5), using the soft liquid storing bag of flexibility of Kynoar PVDF materials；
Only there are one liquid in-out mouth in the nutrient solution bag (1), which passes through straight-through pipe connecting piece and silicone rubber tube (2)
It is connected；Nutrient solution bag (1) is positioned in fixed low temperature box, and box volume fills the body after liquid more than or equal to nutrient solution bag
Product, the inlet of culture unit (3) are connected by silicone rubber tube (2) with nutrient solution bag (1), and liquid outlet passes through silicone rubber tube
(2) it is connected through peristaltic pump (4) with waste fluid bag (5)；Culture unit upper cover can be opened, and be sealed after being packed into cell.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201721316677.1U CN207525252U (en) | 2017-10-13 | 2017-10-13 | Liquid system is changed suitable for what spatial cell was cultivated automatically |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201721316677.1U CN207525252U (en) | 2017-10-13 | 2017-10-13 | Liquid system is changed suitable for what spatial cell was cultivated automatically |
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| CN207525252U true CN207525252U (en) | 2018-06-22 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111197006A (en) * | 2020-01-16 | 2020-05-26 | 杭州键一生物科技有限公司 | CAR-T cell expander |
| CN112608848A (en) * | 2020-12-17 | 2021-04-06 | 清华大学 | Cell suspension culture micro-fluidic device in space environment |
| CN113388500A (en) * | 2021-06-01 | 2021-09-14 | 南京大学 | Cell culture monitoring system and method capable of being used under microgravity |
-
2017
- 2017-10-13 CN CN201721316677.1U patent/CN207525252U/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111197006A (en) * | 2020-01-16 | 2020-05-26 | 杭州键一生物科技有限公司 | CAR-T cell expander |
| CN111197006B (en) * | 2020-01-16 | 2023-09-12 | 杭州键一生物科技有限公司 | CAR-T cell expander |
| CN112608848A (en) * | 2020-12-17 | 2021-04-06 | 清华大学 | Cell suspension culture micro-fluidic device in space environment |
| CN112608848B (en) * | 2020-12-17 | 2022-09-09 | 清华大学 | Cell suspension culture unit suitable for space environment |
| CN113388500A (en) * | 2021-06-01 | 2021-09-14 | 南京大学 | Cell culture monitoring system and method capable of being used under microgravity |
| CN113388500B (en) * | 2021-06-01 | 2024-03-12 | 南京大学 | Cell culture monitoring system and method applicable to microgravity |
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