CN105296354A - Cell culture flask and production method thereof - Google Patents
Cell culture flask and production method thereof Download PDFInfo
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- CN105296354A CN105296354A CN201510238289.5A CN201510238289A CN105296354A CN 105296354 A CN105296354 A CN 105296354A CN 201510238289 A CN201510238289 A CN 201510238289A CN 105296354 A CN105296354 A CN 105296354A
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- culture flask
- culturing bottle
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- bottle body
- tissue culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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Abstract
The invention relates to the field of cell culture, in particular to a cell culture flask and a production method thereof. The cell culture flask comprises a culture flask body, a top wall, a side wall, a bottom wall, a neck, a cover, a heating unit, inner walls, outer walls and a guide channel. A material required by the production of the cell culture flask is glass. The cell culture flask is made in four steps: disposing the outer wall and the inner wall on each of the top wall and the side wall; forming vacuum between each inner wall and the corresponding outer wall, coating the inner side of the top wall with a transparent hydrophobic coating, and enabling the inner side of the top wall to be provided with a plurality of nipple projections; enabling each nipple projection to be provided with a plurality of projections; disposing the heater at the bottom of the culture flask. The cell culture flask with the structure is simple and practical in structure, the bottom of the culture flask can be continuously heated, and the temperature of vapor in the flask can be kept constant, thus generation of mist is avoided, and long-term observation is facilitated.
Description
Technical field
The present invention relates to a kind of Tissue Culture Flask and preparation method thereof, especially the Tissue Culture Flask that hazes in bottle of the effective culturing cell of temperature-adjustable preventing.
Background technology
In biotechnology, cell cultures is a requisite process.Cell cultures is generally that cell is inserted culturing bottle, then is put in incubator and carries out.Culturing bottle to be taken out from incubator during cell cultures and with the growing state of observation of cell under being put into microscope.Temperature under normal circumstances due to incubator is higher than room temperature, and the roof inside at culturing bottle after the hot steam in culturing bottle and the freezing air outside bottle meet forms water smoke, and during observation, water smoke causes light scatter to cause cell imaging unclear, affects observations.Experimenter often adopts minimizing to overcome this problem observing time, can not realize cell and examine under a microscope for a long time.
In sum, be necessary to design the effective culturing cell of a kind of temperature-adjustable and culturing bottle roof inside can be avoided to produce water smoke, to solve the problem.
Summary of the invention
For the technical problem of above-mentioned existence, the invention provides a kind of Tissue Culture Flask and preparation method thereof, the effective culturing cell of temperature can be regulated and culturing bottle roof inside can be avoided to produce water smoke.
In order to solve the problems of the technologies described above, the technical solution used in the present invention is:
A kind of Tissue Culture Flask, comprises culturing bottle body, it is characterized in that: roof and the sidewall of described culturing bottle body include outer wall and inwall; There are some mastoid processes inside described culturing bottle body roof, described mastoid process also has some projections; Water-proof coating is scribbled inside described culturing bottle body roof; Described bottle body bottom is provided with heating unit.
Roof and the sidewall of described culturing bottle body include outer wall and inwall, are vacuum between described outer wall and inwall, form double-layer vacuum structure.
Further, described culturing bottle body diapire is that the solid material of transparent heat conduction is made.Its role is to carry out heat exchange by bottom and the external world, in order to control temperature in bottle.If culturing bottle body wall is double-layer vacuum structure, certainly will be difficult to carry out temperature adjustment to inside.And gather due to bottom and have nutrient solution, its specific heat capacity is comparatively large, even if be placed in freezing air, also not easily there is quenching thus cause steam coagulation.
One as above-mentioned improvement is preferred, and described diapire is that glass is made.Glass transmission, heat conductivility and erosion resistance all meet the requirements.
Further, described culturing bottle body roof inwall is the arc that middle high rim is low.On the roof scribbling hydrophobic coating, aggregate into water droplet rapidly after steam coagulation becomes water smoke, the arc roof that middle high rim is low makes water droplet be rapidly to the slip of roof surrounding, is back to bottom culturing bottle finally by sidewall.
Further, described culturing bottle body roof inwall is arc, and outer wall is plane.The inwall of arc utilizes water droplet to spread, and the outer wall of plane utilizes multiple culturing bottle overlap to prevent, and saves space.
Further, described culturing bottle body roof and sidewall intersection are provided with some diversion trenchs.Accelerate water droplet from roof to sidewall diffusion.
Further, be provided with some mastoid processes inside described culturing bottle body roof, described mastoid process is also provided with some projections, form nanostructure.Described mastoid process base diameter is 8-10 micron, and the spacing between mastoid process is 10-14 micron; Described projection base diameter is 150-250 nanometer.
Described culturing bottle inwall scribbles water-proof coating, forms transparent hydrophobic coating in inner side, avoids water in bottle devaporation to produce water smoke.
Further, described heating unit is circumscribed with heating controller, and described heating controller comprises micro-chip, temperature sensor, display screen and button, and described temperature sensor, display screen are connected with micro-chip respectively with button.Heating unit effect one is with the temperature reaching cell suitable growth for the heating when culturing cell; Two is if there is the phenomenon that hazes when being observation, by heating, fog is evaporated again.
Further, described culturing bottle body is surrounded by roof, diapire, sidewall; Described sidewall extends bottleneck; Bottleneck is provided with opening; Also comprise the bottle cap for closing openings, conveniently utilize microscope to observe from roof.
Another object of the present invention is the preparation method that a kind of Tissue Culture Flask is provided, the effective culturing cell of temperature can be regulated and culturing bottle roof inside can be avoided to produce water smoke.
In order to solve the problems of the technologies described above, the technical solution used in the present invention is, specifically comprises the following steps:
1) by arranging outer wall and inwall on culturing bottle body roof and sidewall, be vacuum between being formed between inside and outside ratio.Roof and the sidewall of described culturing bottle body include outer wall and inwall, are vacuum between described outer wall and inwall, form double-layer vacuum structure.Its role is to carry out heat exchange by bottom and the external world, in order to control temperature in bottle.If culturing bottle body wall is double-layer vacuum structure, certainly will be difficult to carry out temperature adjustment to inside.And gather due to bottom and have nutrient solution, its specific heat capacity is comparatively large, even if be placed in freezing air, also not easily there is quenching thus cause steam coagulation.
2) some mastoid processes are set inside roof, mastoid process arranges some projections again, form nanostructure.Be provided with some mastoid processes inside described culturing bottle body roof, described mastoid process is also provided with some projections, form nanostructure, there is hydrophobicity.Described mastoid process base diameter is 8-10 micron, and the spacing between mastoid process is 10-14 micron; Described projection base diameter is 150-250 nanometer.
3) inside roof, coat water-proof coating, form transparent hydrophobic coating in inner side.Described culturing bottle inwall scribbles water-proof coating, forms transparent hydrophobic coating in inner side, avoids water in bottle devaporation to produce water smoke.
4) bottom culturing bottle, arrange heating unit, the Heating temperature of described thermal outer setting heating controller to heating unit is arranged, and during culturing cell, heating is with the temperature reaching cell suitable growth.Described heating unit is circumscribed with heating controller, and described heating controller comprises micro-chip, temperature sensor, display screen and button, and described temperature sensor, display screen are connected with micro-chip respectively with button.Heating unit effect one is with the temperature reaching cell suitable growth for the heating when culturing cell; Two is if there is the phenomenon that hazes when being observation, by heating, fog is evaporated again.
Further, described heating unit adopts ITO material.
As the further improvement of technique scheme, described culturing bottle also comprises and is arranged on the transparent thermal insulation layer of described bottle top wall outer surface.In order to make culturing bottle top not with contacting external air, be provided with the thermal insulation layer of layer of transparent in culturing bottle top wall outer surface, thermal insulation layer vacuum glass is made.When culturing bottle bottom-heated, culturing bottle top and air insulated, make culturing bottle roof inside be not easy to produce water smoke.
As the further improvement of technique scheme, described culturing bottle also comprises the observed layer being arranged on thermal insulation layer outside surface, and described observed layer makes convex lens.Convex lens are set at the outside surface of transparent thermal insulation layer, directly can carry out preliminary observation to cell tissue, easy to use.
Usefulness of the present invention is: the Tissue Culture Flask with said structure, the double-layer vacuum structure of roof and sidewall is utilized to keep temperature, the hydrophobic coating that roof is smeared, the mastoid process that roof is arranged and supramastoid projection in bottle to make roof have hydrophobicity, avoid fog to produce, be convenient to long-term observation.It is simple and practical, without the need to extra equipment and power.Be provided with heating unit at described bottle body bottom, be circumscribed with heating controller at described heating unit, described heating controller comprises micro-chip, temperature sensor, display screen and button, and described temperature sensor, display screen are connected with micro-chip with button.By arranging heating unit bottom culturing bottle, arrange in the Heating temperature of heating unit outer setting heating controller to heating unit, can continue to heat bottom culturing bottle, the steam temperature in bottle is remained unchanged, prevent water vapour from forming water smoke in culturing bottle roof inside.Culturing bottle of the present invention can not form water smoke in culturing bottle inside, and observation of cell tissue image is clear, and observations is reliable.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Mark in figure: 1 culturing bottle body, 2 roofs, 3 sidewalls, 4 diapires, 5 bottlenecks, 6 bottle caps, 7 heating units, 8 inwalls, 9 outer walls, 10 diversion trenchs.
Fig. 2 is roof micro-structure diagram.
Mark in figure: 2 roofs, 11 mastoid processes, 12 projections.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the preferred embodiments of the present invention are described in detail.The experimental technique of unreceipted actual conditions in preferred embodiment, usually conveniently condition.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
embodiment 1 one kinds of Tissue Culture Flasks
As shown in Figure 1, the present invention includes culturing bottle body 1, described culturing bottle body 1 is surrounded by roof 2, diapire 4, sidewall 3; Described sidewall 3 extends bottleneck 5; Bottleneck 5 is provided with opening; Also comprise the bottle cap 6 for closing openings.The roof 2 of culturing bottle body 1 and sidewall 3 include outer wall 9 and inwall 8; Be vacuum between described outer wall 9 and inwall 8.Culturing bottle body 1 diapire 4 is that the solid material of transparent heat conduction is made, preferably glass.Roof 4 inwall is the arc that middle high rim is low, and outer wall is plane.Preferably some diversion trenchs 10 are set at roof 4 and sidewall intersection.Some mastoid processes 11 are provided with inside described culturing bottle body roof 2; Described mastoid process 11 is also provided with some projections 12.Described mastoid process 11 base diameter is 8 microns, and the spacing between mastoid process 11 is 10 microns; Described projection 12 base diameter is 150 nanometers.Transparent hydrophobic coating is scribbled inside described culturing bottle body roof 2.A heating unit 7 can also be set bottom culturing bottle body 1.
The double-layer vacuum structure of roof 2 and sidewall 3 can play heat insulation effect, when culturing bottle take out in incubator be placed in room temperature time, in bottle can not quenching and make water vapour produce condensation.Solid diapire 4 amasss little with air contact surfaces, and owing to having gathered the high nutrient solution of specific heat capacity, also can not produce excessive too fast impact to the temperature in bottle.
When culturing bottle is in room temperature, the water vapour in bottle is dispersed into the condensation of roof 2 place, the depression part air abrim between mastoid process 11, and so just to form one deck on inside roof very thin being close to, and only has the gas cloud that nano level is thick.This just makes, dimensionally much larger than after the droplets form of this structure is on roof 2, across the air that one deck is very thin, to form several point cantact with the projection 12 in mastoid process 11.Water droplet is formed spherical under the surface tension effects of self, and the roof 2 crossing arc is rapidly to surrounding and slides, and is back to bottom culturing bottle finally by sidewall.In whole process, the condensation of water vapour can not have an impact to light, is conducive to long-time observation.
When culturing bottle is in room temperature, the water vapour in bottle is dispersed into roof 2 and goes out condensation, and due to the existence of hydrophobic coating, water vapour cannot condense into water smoke, but assembles water droplet.Water droplet is rapidly to surrounding by the roof of arc and slides, and is back to bottom culturing bottle finally by sidewall.In whole process, the condensation of water vapour can not have an impact to light, is conducive to long-time observation.
Solid hydrophobic surface sees the wetting property of liquid at solid surface.The infiltration degree of liquid versus solid represents with contact angle, contact angle on surface of coating be greater than 90 ° be called hydrophobic coating.Existing transparent hydrophobic coating common is on the market the TeflonAF that Du Pont produces.
Heating unit 7 adopts ITO material.ITO is conductive glass, and ITO conductive glass is on the basis of sodium calcium base or silicon boryl substrate glass, and utilizing the multiple methods such as sputtering, evaporation to plate, indium oxide layer tin film manufactures.Heating unit is laid in the bottom of culturing bottle, makes ito surface down.
the preparation method of embodiment 2 one kinds of Tissue Culture Flasks
The preparation method of the Tissue Culture Flask of the present embodiment, specifically comprises the following steps:
1) by arranging inwall 8 and outer wall 9 on culturing bottle body 1 roof 2 and sidewall 3, be vacuum between being formed between inside and outside ratio.Be vacuum between described outer wall 9 and inwall 8, form double-layer vacuum structure.Its role is to carry out heat exchange by bottom and the external world, in order to control temperature in bottle.If culturing bottle body 1 wall is double-layer vacuum structure, certainly will be difficult to carry out temperature adjustment to inside.And gather due to bottom and have nutrient solution, its specific heat capacity is comparatively large, even if be placed in freezing air, also not easily there is quenching thus cause steam coagulation.
2) some mastoid processes 11 are set inside roof 2, mastoid process 11 are arranged some projections 12 again, form nanostructure.Be provided with some mastoid processes 11 inside described culturing bottle body 1 roof 2, described mastoid process 11 is also provided with some projections 12, form nanostructure, there is hydrophobicity.Described mastoid process 11 base diameter is 8 microns, and the spacing between mastoid process 11 is 10 microns; Described projection 12 base diameter is 150 nanometers.
3) inside roof 2, coat water-proof coating, form transparent hydrophobic coating in inner side.Described culturing bottle inwall 8 scribbles water-proof coating, forms transparent hydrophobic coating in inner side, avoids water in bottle devaporation to produce water smoke.
4) bottom culturing bottle, arrange heating unit 7, connect heating controller outside described thermal, described heating controller comprises micro-chip, temperature sensor, display screen and button, and described temperature sensor, display screen are connected with micro-chip respectively with button.By arranging the Heating temperature of control to heating unit of heating controller, during culturing cell, heating is with the temperature reaching cell suitable growth.
What finally illustrate is, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (10)
1. a Tissue Culture Flask, comprises culturing bottle body, it is characterized in that: roof and the sidewall of described culturing bottle body include outer wall and inwall; There are some mastoid processes inside described culturing bottle body roof, described mastoid process also has some projections; Water-proof coating is scribbled inside described culturing bottle body roof; Described bottle body bottom is provided with heating unit.
2. a kind of Tissue Culture Flask according to claim 1, is characterized in that: described culturing bottle body diapire is that the solid material of transparent heat conduction is made.
3. a kind of Tissue Culture Flask according to claim 1, is characterized in that: described culturing bottle body roof inwall is the arc that middle high rim is low.
4. a kind of Tissue Culture Flask according to claim 3, is characterized in that: described culturing bottle body roof inwall is arc, and outer wall is plane.
5. a kind of Tissue Culture Flask according to claim 3, is characterized in that: described culturing bottle body roof and sidewall intersection are provided with some diversion trenchs.
6. a kind of Tissue Culture Flask according to claim 1, is characterized in that: described mastoid process base diameter is 8-10 micron, and the spacing between mastoid process is 10-14 micron; Described projection base diameter is 150-250 nanometer.
7. a kind of Tissue Culture Flask according to claim 1, it is characterized in that: described heating unit is circumscribed with heating controller, described heating controller comprises micro-chip, temperature sensor, display screen and button, and described temperature sensor, display screen are connected with micro-chip respectively with button.
8. a kind of Tissue Culture Flask according to claim 1, is characterized in that: described culturing bottle body is surrounded by roof, diapire, sidewall; Described sidewall extends bottleneck; Bottleneck is provided with opening; Also comprise the bottle cap for closing openings.
9., based on the preparation method of a kind of Tissue Culture Flask described in any one of claim 1-8, it is characterized in that, specifically comprise the following steps:
1) by arranging outer wall and inwall on culturing bottle body roof and sidewall, be vacuum between being formed between inside and outside ratio;
2) some mastoid processes are set inside roof, mastoid process arranges some projections again, form nanostructure;
3) inside roof, coat water-proof coating, form transparent hydrophobic coating in inner side;
4) bottom culturing bottle, arrange heating unit, arrange in the Heating temperature of heating unit outer setting heating controller to heating unit, during culturing cell, heating is with the temperature reaching cell suitable growth.
10. the preparation method of a kind of Tissue Culture Flask according to claim 9, is characterized in that, described heating unit adopts ITO material.
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| CN201510238289.5A CN105296354A (en) | 2015-05-12 | 2015-05-12 | Cell culture flask and production method thereof |
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| CN201510238289.5A CN105296354A (en) | 2015-05-12 | 2015-05-12 | Cell culture flask and production method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107904167A (en) * | 2017-11-15 | 2018-04-13 | 中国航天员科研训练中心 | The automatic amplification cultivation equipment of cell |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107904167A (en) * | 2017-11-15 | 2018-04-13 | 中国航天员科研训练中心 | The automatic amplification cultivation equipment of cell |
| CN107904167B (en) * | 2017-11-15 | 2023-06-06 | 中国航天员科研训练中心 | Automatic cell expansion culture equipment |
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Application publication date: 20160203 |