CN204676097U - Antifog Tissue Culture Flask - Google Patents
Antifog Tissue Culture Flask Download PDFInfo
- Publication number
- CN204676097U CN204676097U CN201520302482.6U CN201520302482U CN204676097U CN 204676097 U CN204676097 U CN 204676097U CN 201520302482 U CN201520302482 U CN 201520302482U CN 204676097 U CN204676097 U CN 204676097U
- Authority
- CN
- China
- Prior art keywords
- bottle body
- culturing bottle
- tissue culture
- culture flask
- antifog
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to field of cell culture, in particular to the antifog Tissue Culture Flask of one, described Tissue Culture Flask comprises culturing bottle body, roof, sidewall, diapire, bottleneck, bottle cap, heating unit, inwall, outer wall, diversion trench, and the material requested of preparing of described Tissue Culture Flask is glass; Have the Tissue Culture Flask of said structure, structure is simple and practical, avoids the fog in bottle to produce, is convenient to long-term observation.
Description
Technical field
The utility model relates to a kind of antifog Tissue Culture Flask, especially the Tissue Culture Flask that hazes in bottle of the effective culturing cell of temperature-adjustable preventing.
Background technology
In biotechnology, cell cultures is a requisite process.Cell cultures is generally that cell is inserted culturing bottle, then is put in incubator and carries out.Culturing bottle to be taken out from incubator during cell cultures and with the growing state of observation of cell under being put into microscope.Temperature under normal circumstances due to incubator is higher than room temperature, and the roof inside at culturing bottle after the hot steam in culturing bottle and the freezing air outside bottle meet forms water smoke, and during observation, water smoke causes light scatter to cause cell imaging unclear, affects observations.Experimenter often adopts minimizing to overcome this problem observing time, can not realize cell and examine under a microscope for a long time.
In sum, be necessary to design the effective culturing cell of a kind of temperature-adjustable and culturing bottle roof inside can be avoided to produce water smoke, to solve the problem.
Utility model content
For the technical problem of above-mentioned existence, the utility model provides a kind of antifog Tissue Culture Flask, can regulate the effective culturing cell of temperature and culturing bottle roof inside can be avoided to produce water smoke.
In order to solve the problems of the technologies described above, the technical solution adopted in the utility model is:
A kind of antifog Tissue Culture Flask, comprises culturing bottle body, it is characterized in that: roof and the sidewall of described culturing bottle body include outer wall and inwall; There are some mastoid processes inside described culturing bottle body roof, described mastoid process also has some projections; Water-proof coating is scribbled inside described culturing bottle body roof; Described bottle body bottom is provided with heating unit.
Roof and the sidewall of described culturing bottle body include outer wall and inwall, are vacuum between described outer wall and inwall, form double-layer vacuum structure.
Further, described culturing bottle body diapire is that the solid material of transparent heat conduction is made.Its role is to carry out heat exchange by bottom and the external world, in order to control temperature in bottle.If culturing bottle body wall is double-layer vacuum structure, certainly will be difficult to carry out temperature adjustment to inside.And gather due to bottom and have nutrient solution, its specific heat capacity is comparatively large, even if be placed in freezing air, also not easily there is quenching thus cause steam coagulation.
One as above-mentioned improvement is preferred, and described diapire is that glass is made.Glass transmission, heat conductivility and erosion resistance all meet the requirements.
Further, described culturing bottle body roof inwall is the arc that middle high rim is low.On the roof scribbling hydrophobic coating, aggregate into water droplet rapidly after steam coagulation becomes water smoke, the arc roof that middle high rim is low makes water droplet be rapidly to the slip of roof surrounding, is back to bottom culturing bottle finally by sidewall.
Further, described culturing bottle body roof inwall is arc, and outer wall is plane.The inwall of arc utilizes water droplet to spread, and the outer wall of plane utilizes multiple culturing bottle overlap to prevent, and saves space.
Further, described culturing bottle body roof and sidewall intersection are provided with some diversion trenchs.Accelerate water droplet from roof to sidewall diffusion.
Further, be provided with some mastoid processes inside described culturing bottle body roof, described mastoid process is also provided with some projections, form nanostructure.Described mastoid process base diameter is 8-10 micron, and the spacing between mastoid process is 10-14 micron; Described projection base diameter is 150-250 nanometer.
Described culturing bottle inwall scribbles water-proof coating, forms transparent hydrophobic coating in inner side, avoids water in bottle devaporation to produce water smoke.
Further, described heating unit is circumscribed with heating controller, and described heating controller comprises micro-chip, temperature sensor, display screen and button, and described temperature sensor, display screen are connected with micro-chip respectively with button.Heating unit effect one is with the temperature reaching cell suitable growth for the heating when culturing cell; Two is if there is the phenomenon that hazes when being observation, by heating, fog is evaporated again.
Further, described culturing bottle body is surrounded by roof, diapire, sidewall; Described sidewall extends bottleneck; Bottleneck is provided with opening; Also comprise the bottle cap for closing openings, conveniently utilize microscope to observe from roof.
As the further improvement of technique scheme, described culturing bottle also comprises and is arranged on the transparent thermal insulation layer of described bottle top wall outer surface.In order to make culturing bottle top not with contacting external air, be provided with the thermal insulation layer of layer of transparent in culturing bottle top wall outer surface, thermal insulation layer vacuum glass is made.When culturing bottle bottom-heated, culturing bottle top and air insulated, make culturing bottle roof inside be not easy to produce water smoke.
As the further improvement of technique scheme, described culturing bottle also comprises the observed layer being arranged on thermal insulation layer outside surface, and described observed layer makes convex lens.Convex lens are set at the outside surface of transparent thermal insulation layer, directly can carry out preliminary observation to cell tissue, easy to use.
Usefulness of the present utility model is: the Tissue Culture Flask with said structure, the double-layer vacuum structure of roof and sidewall is utilized to keep temperature, the hydrophobic coating that roof is smeared, the mastoid process that roof is arranged and supramastoid projection in bottle to make roof have hydrophobicity, avoid fog to produce, be convenient to long-term observation.It is simple and practical, without the need to extra equipment and power.Be provided with heating unit at described bottle body bottom, be circumscribed with heating controller at described heating unit, described heating controller comprises micro-chip, temperature sensor, display screen and button, and described temperature sensor, display screen are connected with micro-chip with button.By arranging heating unit bottom culturing bottle, arrange in the Heating temperature of heating unit outer setting heating controller to heating unit, can continue to heat bottom culturing bottle, the steam temperature in bottle is remained unchanged, prevent water vapour from forming water smoke in culturing bottle roof inside.Culturing bottle of the present utility model can not form water smoke in culturing bottle inside, and observation of cell tissue image is clear, and observations is reliable.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Mark in figure: 1 culturing bottle body, 2 roofs, 3 sidewalls, 4 diapires, 5 bottlenecks, 6 bottle caps, 7 heating units, 8 inwalls, 9 outer walls, 10 diversion trenchs.
Fig. 2 is roof micro-structure diagram.
Mark in figure: 2 roofs, 11 mastoid processes, 12 projections.
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, preferred embodiment of the present utility model is described in detail.Should be appreciated that specific embodiment described herein only in order to explain the utility model, and be not used in restriction the utility model.
As shown in Figure 1, the utility model comprises culturing bottle body 1, and described culturing bottle body 1 is surrounded by roof 2, diapire 4, sidewall 3; Described sidewall 3 extends bottleneck 5; Bottleneck 5 is provided with opening; Also comprise the bottle cap 6 for closing openings.The roof 2 of culturing bottle body 1 and sidewall 3 include outer wall 9 and inwall 8; Be vacuum between described outer wall 9 and inwall 8.Culturing bottle body 1 diapire 4 is that the solid material of transparent heat conduction is made, preferably glass.Roof 4 inwall is the arc that middle high rim is low, and outer wall is plane.Preferably some diversion trenchs 10 are set at roof 4 and sidewall intersection.Some mastoid processes 11 are provided with inside described culturing bottle body roof 2; Described mastoid process 11 is also provided with some projections 12.Described mastoid process 11 base diameter is 8 microns, and the spacing between mastoid process 11 is 10 microns; Described projection 12 base diameter is 150 nanometers.Transparent hydrophobic coating is scribbled inside described culturing bottle body roof 2.A heating unit 7 can also be set bottom culturing bottle body 1.
The double-layer vacuum structure of roof 2 and sidewall 3 can play heat insulation effect, when culturing bottle take out in incubator be placed in room temperature time, in bottle can not quenching and make water vapour produce condensation.Solid diapire 4 amasss little with air contact surfaces, and owing to having gathered the high nutrient solution of specific heat capacity, also can not produce excessive too fast impact to the temperature in bottle.
When culturing bottle is in room temperature, the water vapour in bottle is dispersed into the condensation of roof 2 place, the depression part air abrim between mastoid process 11, and so just to form one deck on inside roof very thin being close to, and only has the gas cloud that nano level is thick.This just makes, dimensionally much larger than after the droplets form of this structure is on roof 2, across the air that one deck is very thin, to form several point cantact with the projection 12 in mastoid process 11.Water droplet is formed spherical under the surface tension effects of self, and the roof 2 crossing arc is rapidly to surrounding and slides, and is back to bottom culturing bottle finally by sidewall.In whole process, the condensation of water vapour can not have an impact to light, is conducive to long-time observation.
When culturing bottle is in room temperature, the water vapour in bottle is dispersed into roof 2 and goes out condensation, and due to the existence of hydrophobic coating, water vapour cannot condense into water smoke, but assembles water droplet.Water droplet is rapidly to surrounding by the roof of arc and slides, and is back to bottom culturing bottle finally by sidewall.In whole process, the condensation of water vapour can not have an impact to light, is conducive to long-time observation.
Solid hydrophobic surface sees the wetting property of liquid at solid surface.The infiltration degree of liquid versus solid represents with contact angle, contact angle on surface of coating be greater than 90 ° be called hydrophobic coating.Existing transparent hydrophobic coating common is on the market the Teflon AF that Du Pont produces.
Heating unit 7 adopts ITO material.ITO is conductive glass, and ITO conductive glass is on the basis of sodium calcium base or silicon boryl substrate glass, and utilizing the multiple methods such as sputtering, evaporation to plate, indium oxide layer tin film manufactures.Heating unit is laid in the bottom of culturing bottle, makes ito surface down.
What finally illustrate is, above embodiment is only in order to illustrate the technical solution of the utility model and unrestricted, although be described in detail the utility model with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can modify to the technical solution of the utility model or equivalent replacement, and not departing from aim and the scope of technical solutions of the utility model, it all should be encompassed in the middle of right of the present utility model.
Claims (8)
1. antifog Tissue Culture Flask, comprises culturing bottle body, it is characterized in that: roof and the sidewall of described culturing bottle body include outer wall and inwall; There are some mastoid processes inside described culturing bottle body roof, described mastoid process also has some projections; Water-proof coating is scribbled inside described culturing bottle body roof; Described bottle body bottom is provided with heating unit.
2. antifog Tissue Culture Flask according to claim 1, is characterized in that: described culturing bottle body diapire is that the solid material of transparent heat conduction is made.
3. antifog Tissue Culture Flask according to claim 1, is characterized in that: described culturing bottle body roof inwall is the arc that middle high rim is low.
4. antifog Tissue Culture Flask according to claim 3, is characterized in that: described culturing bottle body roof inwall is arc, and outer wall is plane.
5. antifog Tissue Culture Flask according to claim 3, is characterized in that: described culturing bottle body roof and sidewall intersection are provided with some diversion trenchs.
6. antifog Tissue Culture Flask according to claim 1, is characterized in that: described mastoid process base diameter is 8-10 micron, and the spacing between mastoid process is 10-14 micron; Described projection base diameter is 150-250 nanometer.
7. antifog Tissue Culture Flask according to claim 1, it is characterized in that: described heating unit is circumscribed with heating controller, described heating controller comprises micro-chip, temperature sensor, display screen and button, and described temperature sensor, display screen are connected with micro-chip respectively with button.
8. antifog Tissue Culture Flask according to claim 1, is characterized in that: described culturing bottle body is surrounded by roof, diapire, sidewall; Described sidewall extends bottleneck; Bottleneck is provided with opening; Also comprise the bottle cap for closing openings.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520302482.6U CN204676097U (en) | 2015-05-12 | 2015-05-12 | Antifog Tissue Culture Flask |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520302482.6U CN204676097U (en) | 2015-05-12 | 2015-05-12 | Antifog Tissue Culture Flask |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204676097U true CN204676097U (en) | 2015-09-30 |
Family
ID=54175802
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520302482.6U Expired - Fee Related CN204676097U (en) | 2015-05-12 | 2015-05-12 | Antifog Tissue Culture Flask |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204676097U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105296354A (en) * | 2015-05-12 | 2016-02-03 | 重庆市大足区珠溪中心卫生院 | Cell culture flask and production method thereof |
| CN107338186A (en) * | 2016-05-03 | 2017-11-10 | 华北理工大学 | Microscope cell cultivation equipment |
-
2015
- 2015-05-12 CN CN201520302482.6U patent/CN204676097U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105296354A (en) * | 2015-05-12 | 2016-02-03 | 重庆市大足区珠溪中心卫生院 | Cell culture flask and production method thereof |
| CN107338186A (en) * | 2016-05-03 | 2017-11-10 | 华北理工大学 | Microscope cell cultivation equipment |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107298533B (en) | Method for preparing three-dimensional graphene glass composite material | |
| Tang et al. | Achieving excellent thermal transfer in highly light absorbing conical aerogel for simultaneous passive cooling and solar steam generation | |
| CN204676097U (en) | Antifog Tissue Culture Flask | |
| CN104597648B (en) | A kind of display panels and device | |
| CN104556230B (en) | Molybdenum disulfide nano laurustinus structural semiconductor material and preparation method thereof | |
| CN103667055A (en) | Culture container, culture observation apparatus and culture observation method | |
| CN101693519A (en) | Process for preparing silicon dioxide nano-cone array | |
| CN104409657B (en) | A kind of encapsulation cover plate and its application in Organnic electroluminescent device | |
| CN105112855A (en) | Evaporation crucible and evaporation system | |
| CN104808377B (en) | The method for showing alignment mark position, array base palte and preparation method thereof | |
| CN104078626A (en) | Heating device for OLED (Organic Light Emitting Diode) material evaporation | |
| CN107376948B (en) | A kind of preparation method of two dimension selenizing molybdenum functional material water electrolysis hydrogen production catalyst | |
| Guo et al. | Development of flexible photothermal superhydrophobic microarray by photolithography technology for anti-icing and deicing | |
| CN109911888A (en) | A kind of preparation method and application of zero defect unrest layer stacked graphene nanometer film | |
| CN103484821A (en) | Preparation method for nano volcanic type array thin film with responsiveness pure color displaying function | |
| CN103624853A (en) | Method for preparing wood surface hydrophilicity-hydrophobicity reversible switch | |
| CN105296354A (en) | Cell culture flask and production method thereof | |
| Yong et al. | Mini-review on bioinspired superwetting microlens array and compound eye | |
| Banaha et al. | Spreading of liquid drops on agar gels | |
| Liu et al. | Bioinspired Superhydrophobic All‐in‐one Coating for Adaptive Thermoregulation | |
| CN204676096U (en) | A kind of Tissue Culture Flask of double-layer vacuum structure | |
| CN205710769U (en) | A kind of Tissue Culture Flask being easy to observe | |
| TWI399568B (en) | An electrical control light valve apparatus having liquid gallium | |
| CN204676098U (en) | A kind of heatable culturing bottle | |
| CN202814447U (en) | Device used for observing vacuum boiling phenomenon in electrodeposition microspace |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150930 Termination date: 20160512 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |