CN215887050U - Power device for cell culture - Google Patents

Abstract

The utility model relates to a power device for cell culture, which comprises a pipeline system and a pipeline extrusion power assembly, and is characterized in that the pipeline system comprises a first pipeline, a second pipeline and a third pipeline, a first one-way valve connected with the first pipeline and the second pipeline and a second one-way valve connected with the second pipeline and the third pipeline, the liquid flow directions of the first one-way valve and the second one-way valve are consistent, and the pipeline extrusion power assembly provides liquid flow power for a cell circulating culture pipeline system by extruding the second pipeline.

Description

Power device for cell culture

Technical Field

The utility model relates to the technical field of cell culture, in particular to a power device for cell culture.

Background

Cell culture is essentially large-scale cloning of cells, is widely applied to the fields of biology, medicine, new drug research and development and the like, and becomes one of the most core and basic technologies in biotechnology. There are two main ways for cell culture, adherent culture and suspension culture. Cells cultured by adherence need to be attached to a certain solid phase surface, the occupied area is large, the production efficiency is low, and the cells are not easy to produce on a large scale. With the development of bioreactor technology, the suspension culture of cells by serum-free culture medium and fed-batch culture process has become the mainstream technology. However, the technology still has the problems of shear force damage caused by continuous stirring of cell suspension culture, great difficulty in maintaining the sterile state in the culture process and the like.

Therefore, need a novel cell culture's that is used for power device, the damage that the shearing force brought when it can effectively avoid cell suspension to cultivate, can also avoid polluting because of the pipeline sterilization is not enough, its simple structure, convenient operation can be through dismantling pipeline sterilization recycling, also can regard as the disposable consumptive material because of the pipeline is with low costs simultaneously.

Disclosure of Invention

Aiming at the problems of cell shearing damage and pollution existing in the existing cell culture, the cell perfusion-based culture device provides a power device for cell culture, which has the advantages of simple structure, convenient operation, low price and easy replacement or sterilization.

The purpose of the utility model is realized by the following technical scheme.

The utility model provides a power device for cell culture, includes pipe-line system and pipeline extrusion power component, pipe-line system includes first pipeline, second pipeline, third pipeline, and first check valve of first pipeline, second pipe connection with the second check valve of second pipeline, third pipe connection, first check valve, second check valve liquid flow direction are unanimous, pipeline extrusion power component provides liquid flow power for cell circulation culture pipe-line system through extrusion second pipeline control second pipeline deformation.

Preferably, the second conduit is a hose.

Preferably, the material of the second pipeline is selected from one of polytetrafluoroethylene, meltable polytetrafluoroethylene, aminoplast or silica gel.

Preferably, the second pipe has an inner diameter of 0.2 to 15 mm and a length of 1 to 10 cm.

Further preferably, the inner diameter of the second pipeline is 1-8 mm, and the length of the second pipeline is 3-5 cm.

Preferably, the first pipe and the third pipe are made of air-permeable materials.

Preferably, the first pipeline, the second pipeline and the third pipeline are detachably and hermetically connected with the first one-way valve and the second one-way valve through fixed joints arranged on the first one-way valve and the second one-way valve.

Preferably, the first check valve and the second check valve are any one of a diaphragm check valve, a spring check valve, a gravity check valve and a swing check valve. Preferably, the first check valve and the second check valve are any one of a hose diaphragm check valve, a duckbill check valve and a spring check valve.

Preferably, the first check valve and the second check valve are made of PP, PVDF, PAF, PCF and ABS materials.

Preferably, the pipeline extrusion power assembly comprises one or more extrusion supports and a power driving part.

Preferably, the number of the extrusion supports is two, the two extrusion supports are arranged on two sides of the second pipeline, and the second pipeline is extruded through opposite movement, so that the second pipeline is partially deformed.

Preferably, the power driving part comprises a control unit for controlling the motion state of the extrusion stent, and the control unit adjusts the amplitude and frequency of the extrusion stent, thereby controlling the flow rate of the cell suspension in the pipeline.

The conventional cell suspension culture includes batch culture, fed batch culture, perfusion culture, and the like. The existing culture technology basically adopts a bioreactor, the bioreactor is expensive and needs to be sterilized fully each time the bioreactor is used, otherwise, the pollution of a reaction system is easily caused, and the damage to cells caused by a stirrer often occurs, so that the yield of the cells and/or the metabolites is reduced.

In the utility model, the structure of the pipeline system and the pipeline extrusion power assembly is adopted, so that the cell pipeline culture can be realized, and the bioreactor is not relied on. The power driving part extrudes the bracket to realize pipeline culture of cell suspension in the pipeline, the flow rate of the cell suspension can be effectively controlled, steady operation under low flow rate is realized, and the yield of cells or generated metabolites such as protein and the like is ensured. The culture pipeline system is easy to disassemble and sterilize, the operation flexibility is realized, the pipeline system is made of polytetrafluoroethylene, meltable polytetrafluoroethylene, aminoplast or silica gel, the one-way valve is made of PP and PVDF materials, the price is low, the one-way valve can be used as a disposable consumable material, and the contamination risk caused by insufficient sterilization due to repeated use is effectively avoided.

Drawings

Various other advantages and benefits of the present invention will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the utility model. It is obvious that the drawings described below are only some embodiments of the utility model, and that for a person skilled in the art, other drawings can be derived from them without inventive effort.

In the drawings:

FIG. 1 is a schematic diagram of a power plant for cell culture according to the present invention;

FIG. 2 is a schematic structural diagram of a power device for cell culture according to an embodiment of the present invention.

Detailed Description

Technical solutions and embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. It is apparent that technical solutions and embodiments are described to facilitate understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

It should be noted that certain terms are used throughout the description and claims to refer to particular components. As one skilled in the art will appreciate, various names may be used to refer to a component. In the following description and in the claims, the terms "include" and "comprise" are used in an open-ended fashion, and thus should be interpreted to mean "include, but not limited to. The description which follows is a preferred embodiment of the utility model, but is made for the purpose of illustrating the general principles of the utility model and not for the purpose of limiting the scope of the utility model. The scope of the present invention is defined by the appended claims.

For the purpose of facilitating understanding of the embodiments of the present invention, the following description will be made by taking specific embodiments as examples with reference to the accompanying drawings, and the drawings are not to be construed as limiting the embodiments of the present invention.

FIG. 1 is a schematic diagram showing the structure of a power unit for cell culture according to an embodiment of the present invention. As can be seen from the figure, the power device for cell culture comprises a pipeline system and a pipeline extrusion power assembly, wherein the pipeline system comprises a first pipeline (1), a second pipeline (2) and a third pipeline (3), and a first check valve (4) and a second check valve (5) are connected between the first pipeline (1) and the second pipeline (2) and between the second pipeline (2) and the third pipeline (3). The first pipeline (1), the first one-way valve (4), the second pipeline (2), the second one-way valve (5) and the third pipeline (3) are communicated with each other to form a pipeline passage, wherein the first one-way valve (4) and the second one-way valve (5) control the liquid to flow, and the first one-way valve (4) and the second one-way valve (5) control the liquid to flow in the same direction. The pipeline extrusion power assembly controls the deformation of the second pipeline to provide liquid flow power for the cell circulating culture pipeline system by extruding the second pipeline.

In the embodiment of the present invention, the material of the first check valve (4) and the second check valve (5) is not limited, preferably a non-deformable material, and a material capable of being sterilized at high temperature, so as to reduce the use cost, such as PP, PVDF, PAF, PCF, ABS, etc. The above is merely an embodiment, and is not limited to other materials.

In the embodiment of the present invention, the sizes of the first check valve (4) and the second check valve (5) are not limited.

In one embodiment, the first check valve (4) and the second check valve (5) are any one of a diaphragm check valve, a spring check valve, a gravity check valve and a swing check valve. In a specific embodiment, the first check valve (4) and the second check valve (5) are any one of a hose diaphragm check valve, a duckbill check valve and a spring check valve.

In one embodiment, the second conduit has an internal diameter of 0.2 to 15 mm and a length of 1 to 10 cm. In a specific embodiment, the second pipe has an inner diameter of 1 to 8 mm and a length of 3 to 5 cm.

In one embodiment, the lengths of the first tube (1) and the third tube (3) are not limited, and any desired length can be selected according to the requirements of cell culture, and the inner diameter size is not particularly limited, but is preferably the same as the inner diameter size of the second tube (2) in order to facilitate the flow of the cell suspension in the tube passage.

According to the utility model, the pipeline extrusion power component extrudes the second pipeline (2) to control the second pipeline to deform so as to provide liquid flow power for the cell circulating culture pipeline system, the second pipeline (2) is made of an elastic material, and the second pipeline (2) is a hose. In one embodiment, the material of the second pipe (2) is selected from one of polytetrafluoroethylene, meltable polytetrafluoroethylene, aminoplast or silica gel.

In the utility model, the first pipeline (1) and the third pipeline (3) are both air-permeable pipelines, and the material of the air-permeable pipelines is not limited.

In one embodiment of the utility model, the first pipeline (1), the second pipeline (2) and the third pipeline (3) are detachably and hermetically connected with the first one-way valve (4) and the second one-way valve (5) through fixed joints arranged on the first one-way valve (4) and the second one-way valve (5). The connecting mode facilitates the disassembly and assembly of the pipeline and the one-way valve and is beneficial to pipeline cleaning and sterilization. Of course, a non-detachable integrated structure may be used. The power device for cell culture has the advantages of simple overall structure, easily obtained materials and low cost, and can adopt disposable consumables.

In one embodiment of the utility model, the pipeline extrusion power assembly extrudes the second pipeline (2) to provide liquid flow power for the cell circulating culture pipeline system. The pipeline extrusion power assembly comprises an extrusion support (6) and a power driving part (7). The number of the extrusion supports is one or more. In one embodiment, the two extrusion brackets (6) are respectively arranged at two sides of the second pipeline, and the second pipeline is extruded through opposite movement, so that the pipeline is partially deformed, and the liquid flow is promoted. The deformation of the pipeline part is preferably controlled within the deformation range of 50% -95%.

In the utility model, the extrusion support (6) can be in any shape, the contact area with the second pipeline (2) is designed according to the requirement without limitation, and the extrusion support can ensure that the extrusion pipeline provides enough deformation force. The shape of the extrusion support (6) can be reasonably set by the skilled person according to the requirement. In one embodiment, the pressing bracket (6) can be selected from a rebounding bracket such as a spring structure or a reciprocating bracket.

In the utility model, in order to realize accurate control of the motion state of the extrusion bracket, the power driving part (7) comprises a control unit for controlling the motion state of the extrusion bracket, and the control unit adjusts the motion state of the extrusion bracket, such as motion displacement, amplitude, frequency and the like, so as to control the flow rate and the flow rate of the cell suspension in the pipeline. In one embodiment, the control unit is a main control board, and in another embodiment, the control unit is a single chip microcomputer.

According to the power device for cell culture, the culture pipeline system is easy to disassemble and sterilize, the operation flexibility is realized, the pipeline system is low in price, the power device can be used as a disposable consumable material, and the risk of bacterial contamination caused by insufficient sterilization due to repeated use is effectively avoided. The utility model also forms a cell suspension circulating flow system by combining the structure of the pipeline system and the pipeline extrusion power assembly with the cell culture container, replaces the cell suspension culture stirring function of a bioreactor, and effectively avoids the damage of the stirrer to the shearing force brought by the cell suspension.

Fig. 2 is a schematic structural diagram of an embodiment of the present invention. The utility model is applied to a cell circulating culture device, and the other ends of a first pipeline (1) and a third pipeline (3) are respectively connected with a liquid container (8) for containing cell suspension to form a circulating liquid flow loop to form a cell suspension circulating flow system. In one embodiment, an optical detection assembly and/or a cell separation assembly (9) is disposed on the third conduit (3).

In one embodiment, an air filter membrane is arranged on the top of the liquid container (8), and a liquid guide pipe for adding cell culture liquid can be arranged on the side wall of the liquid container (8).

In one embodiment, the bottom of the liquid container (8) is conical, the other end of the first pipeline (1) is connected with the bottom of the liquid container, and the other end of the third pipeline (3) is connected with the side part or the top of the liquid container. The flow direction of the suspended cells forms a backflow from the first one-way valve to the second one-way valve. In one embodiment, the volume of the liquid container is 10 mL-1L, and more preferably 50 mL-500 mL.

In one embodiment, the optical detection assembly and/or the cell separation assembly (9) comprises an optical detection assembly and/or a cell separation assembly, wherein the optical detection assembly comprises a light absorption detection and/or a fluorescence detection module, the cell separation assembly is used for removing waste culture medium, and the cell separation assembly can be a hollow microporous filter column or a filter assembly with a specific structure, such as a micro-grid filter structure or an Archimedes spiral structure and the like.

In the embodiment of the utility model, the power device for cell circulating culture can realize cell circulating culture by a structure that a pipeline system and a pipeline extrude a power component, avoid the damage to cells caused by a stirrer used in cell suspension culture, effectively control the flow rate of cell suspension by a power driving part, realize steady-state operation at low flow rate and ensure the yield of cells or generated metabolites such as protein and the like. The cultivation pipeline system in this device easily dismantles the sterilization, and the low price can regard as disposable consumptive material, effectively avoids because of the multiple use leads to the not abundant microbiological contamination risk that brings of sterilization.

The present application is susceptible to various modifications and alternative forms, specific embodiments have been shown by way of example in the drawings and have been described in detail herein. However, the application is not intended to be limited to the particular forms disclosed. On the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the scope of the application, which is defined by the appended claims and their legal equivalents.

Claims (10)

1. The utility model provides a power device for cell culture, includes pipe-line system and pipeline extrusion power component, its characterized in that, pipe-line system includes first pipeline, second pipeline, third pipeline, and first pipeline, second pipe connection's first check valve with second pipeline, third pipe connection's second check valve, first check valve, second check valve liquid flow syntropy, pipeline extrusion power component provides liquid flow power for cell circulation culture pipe-line system through the extrusion second pipeline.

2. The power plant of claim 1, wherein the second conduit is a hose.

3. The power plant of claim 2, wherein the second conduit is made of a material selected from the group consisting of polytetrafluoroethylene, meltable polytetrafluoroethylene, aminoplast, and silicone.

4. A power plant according to claim 2, characterized in that the second conduit has an internal diameter of 0.2-15 mm and a length of 1-10 cm.

5. The power plant of claim 1, wherein the first and third conduits are of an air permeable material.

6. The power device according to claim 1, characterized in that the first pipeline, the second pipeline and the third pipeline are detachably and hermetically connected with the first one-way valve and the second one-way valve through fixed joints arranged on the first one-way valve and the second one-way valve.

7. The power plant of claim 1, wherein the first one-way valve and the second one-way valve are any one of a diaphragm one-way valve, a spring one-way valve, a gravity one-way valve and a swing one-way valve.

8. The powerplant of claim 1, wherein the pipeline extrusion power assembly comprises one or more extrusion brackets and a power drive component.

9. The power unit according to claim 8, wherein the pressing brackets are two and provided at both sides of the second pipe to press the second pipe by a facing motion.

10. The power plant of claim 9, wherein the opposed movement of the crush brackets crush the second conduit to partially deform the second conduit.

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