CN204848881U - Simple and easy airlift bioreactor is used in teaching - Google Patents
Simple and easy airlift bioreactor is used in teaching Download PDFInfo
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- CN204848881U CN204848881U CN201520562519.9U CN201520562519U CN204848881U CN 204848881 U CN204848881 U CN 204848881U CN 201520562519 U CN201520562519 U CN 201520562519U CN 204848881 U CN204848881 U CN 204848881U
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Abstract
The utility model discloses a simple and easy airlift bioreactor is used in teaching relates to microbiology technical field. Including the fermentation cylinder, the fermentation cylinder top is equipped with the cover, the air -breather includes the air induction conduit, and bottom in cover to the fermentation cylinder is passed to air induction conduit gas outlet one end, the air inlet one end of air induction conduit lie in the fermentation cylinder outside and the filter membrane of ventilating has set gradually, ventilate rate controller and air pump, the air pump lies in air inlet department, exhaust apparatus includes exhaust manifolds, and top in cover to the fermentation cylinder is passed to exhaust manifolds one end, and the other end of exhaust manifolds is located the fermentation cylinder outside and has set gradually exhaust filter membrane and pressure controller, be equipped with the inoculation sampling pipe on the cover, inoculation sampling pipe one end lies in the fermentation cylinder lower part, and the other end lies in the fermentation cylinder outside, and inoculation sampling pipe top mouth of pipe department is equipped with inoculation sampling stopcock. The utility model discloses small, with low costs, controllable speed and jar pressure of ventilating of reactor is easily installed and is dismantled, is fit for more student experiment course simultaneously.
Description
Technical field
The utility model relates to microbiology device technique field.
Background technology
At present, most of colleges and universities are when submerged fermentation experimental teaching, aerobic microbiological is fermented, sterile air or the controlled small-sized fermentation tank of Ventilation Rate can be passed into owing to lacking, substitute mainly with Erlenmeyer flask and carry out shake-flask culture, and this experimental teaching can not allow student's profound understanding metabolic control fermentation mechanism, the general Production Flow Chart being familiar with fermentation and skilled grasp reach the object of deep layer fermenting process, especially understand dissolved oxygen importance during the fermentation.Therefore, design a kind of small volume, cost is low, be applicable to more student carries out experimental courses simultaneously, the fermentor tank that is applicable to the liquid fermenting that need control Ventilation Rate, be the current technical issues that need to address.
Utility model content
The technical problems to be solved in the utility model is to provide a kind of teaching and uses simple and easy airlift bioreactor, and this bio-reactor volume is little, cost is low, controlled Ventilation Rate and tank pressure, is easy to installation and removal, is applicable to more student and carries out experimental courses simultaneously.
For solving the problems of the technologies described above, technical solution adopted in the utility model is: simple and easy airlift bioreactor is used in a kind of teaching, and comprise fermentor tank, the top of fermentor tank is provided with cover, also comprises breather, gas barrier and inoculation sampling apparatus;
Breather comprises air-intake duct, Ventilation Rate controller, vent filter and air pump; One end, air outlet of air-intake duct is through cover to fermentor tank inner bottom part, and inlet mouth one end of air-intake duct to be positioned at outside fermentor tank and to be disposed with vent filter, Ventilation Rate controller and air pump, and air pump is positioned at inlet mouth place;
Gas barrier comprises exhaust guide, exhaust filter membrane and pressure controller; One end of exhaust guide is through cover to fermentor tank inner top, and the other end of exhaust guide to be positioned at outside fermentor tank and to be disposed with exhaust filter membrane and pressure controller;
Inoculation sampling apparatus comprises inoculation sampling tube and inoculation sampling pipe close; Cover is provided with inoculation sampling tube, and one end of inoculation sampling tube is positioned at fermentor tank bottom, and the other end is positioned at outside fermentor tank, and inoculation mouth of pipe place, sampling tube top is provided with inoculation sampling pipe close.
Preferably, the air outlet place of air-intake duct is provided with Bubbled stone.
Preferably, inoculation sampling apparatus also comprises connection plug, and inoculation sampling tube is connected with cover by connection plug.
Preferred further, the material of inoculation sampling pipe close and connection plug is rubber.
Preferably, Ventilation Rate controller and pressure controller are transfusion device flow regulator.
Preferably, fermentor tank is right cylinder, and the material of fermentor tank is colourless transparent plastic or flint glass.
Preferably, the aperture of vent filter and exhaust filter membrane is 0.22 μm.
Preferably, the top inoculating sampling tube is doline drum.
The beneficial effect adopting technique scheme to produce is:
(1) the utility model volume is little, cost is low, controlled Ventilation Rate and tank pressure, is easy to installation and removal, is applicable to more student and carries out experimental courses simultaneously;
(2) design of Ventilation Rate controller and pressure controller makes the controlled Ventilation Rate of this bio-reactor and tank pressure; Fermentor tank is colourless transparent plastic or flint glass material, is applicable to experimental teaching, simple and practical, with low cost; The design of Bubbled stone makes air be discharged by Bubbled stone, produces a large amount of bubble; The material of inoculation sampling pipe close and connection plug is rubber, utilizes the inoculation sampling tube that connection plug flexible fastening is passed, and inoculation sampling pipe close can be accessed by pressure and measures pipe or asepsis injector; By regulating the position of connection plug on inoculation sampling tube, the degree of depth of adjustable inoculation sampling tube end in fermented liquid, thus adjustable sampling depth, can adapt to more test demand.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the utility model is described in further detail;
Fig. 1 is structural representation of the present utility model;
Fig. 2 is pressure measurement and adjustment using state schematic diagram in the utility model tank;
Fig. 3 is that the utility model air flow measures and regulates using state schematic diagram;
Fig. 4 is the utility model inoculation sampling using state schematic diagram;
In figure, 1, fermentor tank; 2, connection plug; 3, sampling tube is inoculated; 4, inoculation sampling pipe close; 5, cover; 6, filter membrane is vented; 7, vent filter; 8, exhaust guide; 9, pressure controller; 10, Ventilation Rate controller; 11, air-intake duct; 12, inlet mouth; 13, air pump; 14, air outlet; 15, Bubbled stone; 16, pressure measures pipe; 17, air flow measures bottle; 18, air flow measures groove; 19, asepsis injector.
Embodiment
Embodiment 1
Simple and easy airlift bioreactor is used in a kind of teaching, and as shown in Figure 1, comprise fermentor tank 1, the top of fermentor tank 1 is provided with cover 5, also comprises breather, gas barrier and inoculation sampling apparatus.Fermentor tank 1 is right cylinder, and the material of fermentor tank 1 is colourless transparent plastic or flint glass.
Breather comprises air-intake duct 11, Ventilation Rate controller 10, vent filter 7 and air pump 13; One end, air outlet 14 of air-intake duct 11 is through cover 5 to fermentor tank 1 inner bottom part, and inlet mouth 12 one end of air-intake duct 11 to be positioned at outside fermentor tank 1 and to be disposed with vent filter 7, Ventilation Rate controller 10 and air pump 13, and air pump 13 is positioned at inlet mouth 12 place.Air outlet 14 place of air-intake duct 11 is provided with Bubbled stone 15.Air pump 13 pumps into air, by air-intake duct 11, controls air flow rate by rate controller 10, after vent filter 7 is degerming, enters bottom fermentor tank 1, is discharged by Bubbled stone 15, produce a large amount of bubble along air-intake duct 11.
Gas barrier comprises exhaust guide 8, exhaust filter membrane 6 and pressure controller 9; One end of exhaust guide 8 is through cover 5 to fermentor tank 1 inner top, and the other end of exhaust guide 8 to be positioned at outside fermentor tank 1 and to be disposed with exhaust filter membrane 6 and pressure controller 9.Ventilation Rate controller 10 and pressure controller 9 are transfusion device flow regulator.The aperture of vent filter 7 and exhaust filter membrane 6 is 0.22 μm.
Inoculation sampling apparatus comprises inoculation sampling tube 3, inoculation sampling pipe close 4 and connection plug 2, and inoculation sampling tube 3 is connected with cover 5 by connection plug 2.One end of inoculation sampling tube 3 is positioned at fermentor tank 1 bottom, and the other end is positioned at outside fermentor tank 1, and inoculation mouth of pipe place, sampling tube 3 top is provided with inoculation sampling pipe close 4.The material of inoculation sampling pipe close 3 and connection plug 2 is rubber.The top of inoculation sampling tube 3 is doline drum.
This bio-reactor using method comprises pressure in tank, ventilation measurements and control method, the using method of inoculation sampling apparatus.
Pressure measurement and control method in tank, as shown in Figure 2,1L water is added in fermentor tank 1, after this bio-reactor is assembled, inoculation sampling tube 3 connects a pressure and measures Glass tubing or the hard plastic tube that pipe 16(pressure mensuration pipe 16 is afterbody wedge shape), connect air pump 13 power supply, after liquid level stabilizing in pressure mensuration pipe 16, measure and liquid level difference H in fermentor tank 1, try to achieve the internal pressure of this bio-reactor according to P=ρ gH.By the internal pressure regulating pressure controller 9 to regulate this bio-reactor.
Air flow measures and control method, as shown in Figure 3,1L water is added in fermentor tank 1, after bio-reactor is assembled, one air flow is measured bottle 17(air flow and measures bottle 17 for graduated cylinder or volumetric flask) fill water after, be inverted in the air flow filling suitable quantity of water and measure groove 18(air flow and measure groove 18 for tank or beaker) in.Connect air pump 13 power supply, after steady air current, exhaust guide 8 end is placed in air flow and measures in bottle 17, and start timing, to measure in the unit time ventilation volume or pass into the certain volume time used, calculating Ventilation Rate.By the Ventilation Rate regulating Ventilation Rate controller 10 to regulate this bio-reactor.
The method inoculated during the fermentation and sample, as shown in Figure 4, uses asepsis injector 19 to realize inoculation and sampling by inoculating sampling tube 3, microbiological contamination chance when significantly reducing traditional way inoculation or sample.By regulating the position of connection plug 2 on inoculation sampling tube 3, the degree of depth of adjustable inoculation sampling tube 3 end in fermented liquid, thus adjustable sampling depth, can adapt to more test demand.
Embodiment 2: the application example of this bio-reactor.
1: fermentation of Aspergillus niger
Actication of culture.To go bail for Tibetan aspergillus niger strain, with a little spore inoculating of transfering loop picking in slant tube, constant temperature culture 3d at 35 DEG C.
Prepared by shake-flask seed.When substratum temperature is down to below 40 DEG C, inoculate in substratum by the aspergillus niger spore of activation, access amount should reach 0.6 ~ 1.5 ten thousand/mL, at 35 DEG C, about 20h in the shaking table of 200/min, mycelium pellet is fine and close shape, and bacterium spherical diameter should more than 0.1mm, and mycelia is short, and it is sturdy, branch is few, warty, and demi-inflation is excellent.
Fermentation culture.Regulate the pH of substratum between 2 ~ 4, get 1000mL substratum and add fermentor tank 1, sterilizing after this bio-reactor is assembled, when substratum temperature is down to below 30 DEG C, air-intake duct 11 is connected with air pump 13, connect air pump 13 power supply, regulate Ventilation Rate controller 10, pressure controller 9,5.0cm is provided
3the ventilation condition of/min also ensures that in fermentor tank 1, pressure is 1 ~ 3KPa, uses asepsis injector 19 to access ferment-seeded 50mL by inoculation sampling tube 3, is placed in constant incubator 28-30 DEG C cultured continuously 72h, pending.
Fermenting process detects.After inoculation, use asepsis injector 19 is sampled by inoculation sampling tube 3 and does to measure for the first time, measures once per half a day, until end later.Mensuration project has: reducing sugar, total reducing sugar, acidity, citric acid measure, microscopy.
2:beer production
Get 1000mL substratum and add fermentor tank 1, sterilizing after this bio-reactor is assembled, when substratum temperature is down to below 30 DEG C, air-intake duct 11 is connected with air pump 13, connect air pump 13 power supply, regulate Ventilation Rate controller 10 to provide appropriate oxygen, use asepsis injector 19 to access the bacterial classification of yeast 100ml containing 1*108 yeast/ml by inoculation sampling tube 3, be placed in constant incubator 10 DEG C and cultivate 20h.
Enter Primary Fermentation afterwards, oxygen feeding stop, product temperature should control to be advisable at 8 ~ 12 DEG C, and fermentation time is about through about 5 ~ 7 days.
Naturally be warming up to 12 DEG C, and close Ventilation Rate controller 10, pressure controller 9, utilize thalline to produce carbonic acid gas and improve pressure in tank, the hold-time is 4 days.If without obvious di-acetyl taste, can lower the temperature, if there is obvious di-acetyl taste, cooling in 1 ~ 3 day can be postponed.
Secondary fermentation, after reduction terminates, was cooled to 2 ~ 3 DEG C by regulation and keeps tank internal pressure at about 0.1MPa, keeping 3 ~ 5 days in 24 hours.
Fermenting process detects.After inoculation, use asepsis injector 19 is sampled by inoculation sampling tube 3 and does to measure for the first time, measures once per half a day, until end later.Mensuration project has: total reducing sugars, cell concn, bud ratio, rate of dyeing, acidity and pH, α-amino nitrogen, colourity.
The utility model mainly affects two principal elements (i.e. pressure, Ventilation Rate in tank) design of dissolved oxygen according to oxygen supply aspect, and main according to oxygen equation of transfer OTR=K
la(C
*-C
l), by pressure in regulating tank, Ventilation Rate and change K
la, C
*, fermentation oxygen supply is improved.With reference to fermentor tank design ultimate principle, appropriate design is carried out to one-piece construction in addition.
In formula: the oxygen transfer rate of OTR--unit volume nutrient solution;
K
la--take concentration difference as the volume dissolved oxygen coefficient of impellent;
C
*--with the concentration of oxygen when oxygen partial pressure p balances each other in meteorology;
C
l--the concentration of gentle in liquid phase, liquid interface oxygen.
The utility model volume is little, cost is low, controlled Ventilation Rate and tank pressure, is easy to installation and removal, is applicable to more student and carries out experimental courses simultaneously.Its using method comprises pressure in tank, ventilation measurements and control method, the using method of inoculation sampling apparatus.
Claims (8)
1. a simple and easy airlift bioreactor is used in teaching, and comprise fermentor tank (1), the top of fermentor tank (1) is provided with cover (5), it is characterized in that: also comprise breather, gas barrier and inoculation sampling apparatus;
Described breather comprises air-intake duct (11), Ventilation Rate controller (10), vent filter (7) and air pump (13); Cover (5) is passed to fermentor tank (1) inner bottom part in air outlet (14) one end of air-intake duct (11), inlet mouth (12) one end of air-intake duct (11) is positioned at fermentor tank (1) outside and is disposed with vent filter (7), Ventilation Rate controller (10) and air pump (13), and air pump (13) is positioned at inlet mouth (12) place;
Described gas barrier comprises exhaust guide (8), exhaust filter membrane (6) and pressure controller (9); One end of exhaust guide (8) is through cover (5) to fermentor tank (1) inner top, and the other end of exhaust guide (8) is positioned at fermentor tank (1) outside and is disposed with exhaust filter membrane (6) and pressure controller (9);
Described inoculation sampling apparatus comprises inoculation sampling tube (3) and inoculation sampling pipe close (4); Cover (5) is provided with inoculation sampling tube (3), and one end of inoculation sampling tube (3) is positioned at fermentor tank (1) bottom, and the other end is positioned at fermentor tank (1) outside, and inoculation mouth of pipe place, sampling tube (3) top is provided with inoculation sampling pipe close (4).
2. simple and easy airlift bioreactor is used in one teaching according to claim 1, and it is characterized in that, air outlet (14) place of air-intake duct (11) is provided with Bubbled stone (15).
3. simple and easy airlift bioreactor is used in one teaching according to claim 1, and it is characterized in that, inoculation sampling apparatus also comprises connection plug (2), and inoculation sampling tube (3) is connected with cover (5) by connection plug (2).
4. simple and easy airlift bioreactor is used in one teaching according to claim 3, and it is characterized in that, the material of inoculation sampling pipe close (3) and connection plug (2) is rubber.
5. simple and easy airlift bioreactor is used in one teaching according to claim 1, and it is characterized in that, Ventilation Rate controller (10) and pressure controller (9) are transfusion device flow regulator.
6. simple and easy airlift bioreactor is used in one teaching according to claim 1, and it is characterized in that, fermentor tank (1) is right cylinder, and the material of fermentor tank (1) is colourless transparent plastic or flint glass.
7. simple and easy airlift bioreactor is used in one teaching according to claim 1, and it is characterized in that, the aperture of vent filter (7) and exhaust filter membrane (6) is 0.22 μm.
8. simple and easy airlift bioreactor is used in one teaching according to claim 1, and it is characterized in that, the top of inoculation sampling tube (3) is doline drum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520562519.9U CN204848881U (en) | 2015-07-30 | 2015-07-30 | Simple and easy airlift bioreactor is used in teaching |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520562519.9U CN204848881U (en) | 2015-07-30 | 2015-07-30 | Simple and easy airlift bioreactor is used in teaching |
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| CN201520562519.9U Expired - Fee Related CN204848881U (en) | 2015-07-30 | 2015-07-30 | Simple and easy airlift bioreactor is used in teaching |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108102879A (en) * | 2018-01-23 | 2018-06-01 | 河海大学 | A kind of separator of Microcystis aeruginosa morphologic species and the separation method of Microcystis aeruginosa morphologic species |
| EP3728550B1 (en) | 2018-08-15 | 2021-02-17 | Applikon Biotechnology B.V. | Lid configuration for a bioreactor |
| US11511240B2 (en) * | 2019-05-24 | 2022-11-29 | Jonathan Ross Anson | Beverage aeration apparatus |
-
2015
- 2015-07-30 CN CN201520562519.9U patent/CN204848881U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108102879A (en) * | 2018-01-23 | 2018-06-01 | 河海大学 | A kind of separator of Microcystis aeruginosa morphologic species and the separation method of Microcystis aeruginosa morphologic species |
| CN108102879B (en) * | 2018-01-23 | 2019-07-16 | 河海大学 | A kind of separator of Microcystis aeruginosa morphologic species and the separation method of Microcystis aeruginosa morphologic species |
| EP3728550B1 (en) | 2018-08-15 | 2021-02-17 | Applikon Biotechnology B.V. | Lid configuration for a bioreactor |
| JP2021526846A (en) * | 2018-08-15 | 2021-10-11 | アプリコン・バイオテクノロジー・ベスローテン・ヴェンノートシャップＡｐｐｌｉｋｏｎ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ Ｂ．Ｖ． | Closure configuration for bioreactor |
| US11511240B2 (en) * | 2019-05-24 | 2022-11-29 | Jonathan Ross Anson | Beverage aeration apparatus |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151209 Termination date: 20200730 |
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| CF01 | Termination of patent right due to non-payment of annual fee |