CN110684658A - Culture solution container storage device in three-dimensional perfusion type cell culture instrument - Google Patents
Culture solution container storage device in three-dimensional perfusion type cell culture instrument Download PDFInfo
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- CN110684658A CN110684658A CN201911035492.7A CN201911035492A CN110684658A CN 110684658 A CN110684658 A CN 110684658A CN 201911035492 A CN201911035492 A CN 201911035492A CN 110684658 A CN110684658 A CN 110684658A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M23/48—Holding appliances; Racks; Supports
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
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Abstract
The invention provides a culture solution container storage device in a three-dimensional perfusion type cell culture instrument, which comprises: the bottom of the inner side of the installation cavity on two sides of the incubator shell is provided with a bearing piece; the bracket is provided with a horizontally arranged mounting plate, the upper surface of the mounting plate is provided with a mounting part, the bottom surface of the mounting plate is provided with a sliding part, and the sliding part is sleeved on the bearing part; the bracket is driven by external force to do reciprocating linear translation motion on the bearing piece through the sliding part so as to drive the bracket to extend out of the mounting cavity or retreat into the mounting cavity. The storage device solves the problems of difficult operation and easy pollution caused by narrow space when the liquid conveying pipe is connected with the culture solution container and the peristaltic pump, and improves the efficiency of cell culture work. In addition, provide enough big space for cell culture liquid container locker room, the convenience is at different culture periods, through the different nutrient composition of bacterial filter filling or cytokine on the compound hole bottle lid of blake bottle to the realization is to the accurate control of cell culture condition.
Description
Technical Field
The invention relates to the technical field of bioengineering, in particular to a culture solution container storage device in a three-dimensional perfusion type cell culture instrument.
Background
Cell culture technology has become the basic technology and basic skill in various research fields of life science at present, and is an important research means of cell engineering, genetic engineering and biomedical engineering. Cell culture technology is the most central and basic technology in biotechnology. In a three-dimensional perfusion cell culture apparatus, when multi-cell simultaneous culture is performed, a plurality of culture solution supply bottles and a plurality of culture solution waste bottles are provided, which are respectively connected with a reactor through infusion tubes, so that unidirectional continuous perfusion of tissue solution is realized, and proliferation and differentiation of tissue cells are further realized. These cell culture liquid containers are generally placed in the installation cavity of cell culture appearance through artifical mode to carry out being connected of transfer line and peristaltic pump in the installation cavity, because the installation cavity space is narrow and small, directly wherein when manually placing cell culture liquid container and connecting the peristaltic pump through the transfer line, can lead to the operating difficulties, the easy emergence pollutes to and transfer line connection mistake, cause the cell culture inefficacy, reduce the efficiency of cell culture work. In addition, different nutrients or cytokines need to be added through the bacterial filter on the multi-hole bottle cap of the culture bottle in different culture periods, so that the precise control of the cell culture conditions is realized, and the storage chamber of the cell culture solution container needs to have enough space.
Disclosure of Invention
In view of the above, the invention provides a culture solution container storage device in a three-dimensional perfusion type cell culture instrument, which is used for solving the problems that in the prior art, due to the narrow space of a cell culture instrument installation cavity, the operation is difficult when a liquid conveying pipe is connected with a culture solution container and a peristaltic pump, and the pollution is easy to occur. Moreover, the cell culture solution container storage chamber needs to have a large enough space so that different nutrients or cytokines can be added through the bacterial filter on the compound-pore bottle cap of the culture bottle in different culture periods, and accurate control of cell culture conditions can be conveniently realized.
In order to achieve the purpose, the invention provides the technical scheme that:
a culture solution container storage device in a three-dimensional perfusion type cell culture instrument comprises:
the culture instrument comprises a culture instrument shell, wherein mounting cavities are formed in two sides of the culture instrument shell, and bearing parts are arranged at the bottom of the inner side of each mounting cavity;
the bracket is provided with a horizontally arranged mounting plate, the top surface of the mounting plate is provided with a mounting part for placing a cell culture solution container, the bottom surface of the mounting plate is fixedly provided with a sliding part, and the sliding part is sleeved on the bearing piece;
the bracket is driven by external force to do reciprocating linear translation motion on the bearing piece through the sliding part so as to drive the bracket to extend out of or retreat into the mounting cavity.
On the basis of the technical scheme, the installation structure is preferred to further comprise a liquid level sensor, and the liquid level sensor is arranged on the installation plate on one side of the installation part.
On the basis of the technical scheme, preferably, a power source is fixedly arranged at the bottom of the inner side of the installation cavity and used for driving the sliding part at the bottom of the installation plate to perform linear translation motion on the bearing piece.
On the basis of above technical scheme, it is preferred, the power supply is telescopic machanism, telescopic machanism's stiff end with installation cavity inboard bottom is fixed connection, telescopic machanism's expansion end with the mounting panel is connected, through telescopic machanism's expansion end's flexible volume is in with the drive the sliding part is in do rectilinear translation motion on bearing the piece.
On the basis of the above technical solution, preferably, the bearing member is a pair of sliding rods fixedly arranged at the bottom inside the installation cavity.
On the basis of the above technical solution, preferably, the telescopic mechanism is any one of a hydraulic cylinder, an air cylinder or an electric cylinder.
Further preferably, the bearing parts are a pair of screw rods arranged at the bottom of the inner side of the mounting cavity, the power source is a servo motor, an output shaft of the servo motor is fixedly connected with one end of the screw rod, and the sliding part is in threaded connection with the screw rod.
On the basis of the above technical scheme, preferably, the bracket further comprises a baffle plate vertically connected with the mounting plate, and the baffle plate is used for covering the opening of the mounting cavity.
On the basis of the technical scheme, preferably, a handle is arranged on the outer side wall of the baffle, and the bracket extends out of or retracts into the mounting cavity under the driving of the handle.
On the basis of above technical scheme, it is preferred, the fixed spacing portion that is provided with on the installation cavity inboard bottom, hold the one end of carrier and rotate and connect on installation cavity opening lateral wall, hold the other end of carrier and rotate and connect in spacing portion.
Compared with the prior art, the invention has the following beneficial effects:
(1) according to the culture solution container storage device in the three-dimensional perfusion type cell culture instrument, the installation cavity is formed in one side of the culture instrument body, the bearing piece is arranged at the bottom of the inner side of the installation cavity, the bracket is arranged, the installation part arranged on the top surface of the installation plate on the bracket is used for placing the cell culture solution container, and the sliding part arranged at the bottom of the installation plate can perform linear translation motion along the bearing piece. When the cell culture solution needs to be added or replaced in the container, the storage device can manually extend or retract the bracket from the installation cavity, and can also realize automatic operation through a power source in the installation cavity, so that the bracket drives the cell culture solution container to automatically extend or retract from the installation cavity, and the bracket has a wider space after extending out of the installation cavity, thereby facilitating the fixed placement of the cell culture solution container on the bracket; meanwhile, the disassembly and replacement are convenient, when the cell culture solution container is connected with the peristaltic pump through the infusion tube, the ordered operation of the infusion tube is convenient, and the pollution is avoided. Finally, the storage chamber of the cell culture solution container needs to have a large enough space so as to manually inject different nutrients or cytokines through the bacterial filter on the multi-hole bottle cap of the culture bottle in different culture periods, thereby realizing the precise control of the cell culture conditions.
(2) According to the invention, by arranging the liquid level sensor, the liquid level change of the culture solution or the cell waste liquid in the culture solution container can be detected, and the alarm prompt is carried out in advance, so that an experimenter can timely carry out operations such as adding, replacing or dumping on the culture solution in the culture solution container, and the practicability is stronger.
Compared with the prior art, the cell culture solution container storage device disclosed by the invention can be conveniently fixed and placed when the cell culture solution container is stored in the three-dimensional perfusion cell culture instrument, is convenient to disassemble and replace, and improves the efficiency of cell culture work. In addition, the invention provides a large enough space for the storage chamber of the cell culture solution container, and different nutrient components or cell factors can be conveniently and manually injected through the bacterial filter on the compound-hole bottle cap of the culture bottle in different culture time periods, thereby realizing the accurate control of the cell culture conditions.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a perspective view of a culture medium container storage device in a three-dimensional perfusion type cell culture apparatus disclosed in the present invention;
FIG. 2 is a schematic view of the mounting of a culture solution container on a bracket in the three-dimensional perfusion type cell culture apparatus disclosed in the present invention;
FIG. 3 is a schematic structural diagram of a mounting cavity according to a first embodiment of the disclosure;
fig. 4 is a schematic structural view of the installation cavity of the second embodiment disclosed in the present invention.
Reference numerals:
100-incubator shell, 110-installation cavity, 120-bearing part, 200-bracket, 210-installation plate, 220-installation part, 230-sliding part, 300-power source, 310-telescoping mechanism, 121-sliding rod, 122-screw rod, 320-servo motor, 240-baffle, 250-handle, 130-limiting part and 140-liquid level sensor.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
As shown in fig. 1 and in combination with fig. 2, an embodiment of the present invention discloses a culture solution container storage device in a three-dimensional perfusion type cell culture apparatus, including: the incubator casing 100 is provided with mounting cavities 110 on two sides, the mounting cavities 110 are horizontally arranged on one side of the incubator casing 100, and the bottom of the inner side of the mounting cavities 110 is provided with a bearing piece 120; the bracket 200 has a horizontally disposed mounting plate 210, and a mounting portion 220 for placing a cell culture solution container is provided on the top surface of the mounting plate 210. In this embodiment, the cell culture solution container may be configured as a bottle or a tank for storing a culture solution used for cell culture, a washing solution, or a waste solution generated during cell culture. As the prior art, the cell culture solution containers are provided with the double-hole covers, and the bacterial filters are arranged on the double-hole covers, so that the air pressure inside and outside the containers is consistent after the cell culture solution containers are continuously sucked and removed, and the suction is smoothly carried out. In addition, different nutrient components or cell factors can be conveniently filled into the container in different culture periods, so that the culture conditions can be accurately controlled. In this embodiment, the cell culture solution container in the mounting cavity 110 on one side can contain a culture solution required for cell reaction, the cell culture solution container in the mounting cavity 110 on the other side can be used for containing a cell waste solution generated in the cell reaction process, and the mounting portion 220 can be configured into a corresponding groove according to the shape of the cell culture solution container to fix the cell culture solution container. When placing cell culture liquid container, can fix in inserting the recess of installation department 220 with the bottom of cell culture liquid container, convenient dismantlement. In this embodiment, one or more mounting portions 220 may be provided, and the bottom surface of the mounting plate 210 is fixedly provided with a sliding portion 230, in this embodiment, the sliding portion 230 is a slider, and the sliding portion 230 is sleeved on the bearing member 120; the carriage 200 is driven by an external force to make a reciprocating linear translation motion on the carrier 120 by the sliding portion 230, so as to drive the carriage 200 to extend out of or retract into the mounting cavity 110. By adopting the technical scheme, the cell culture solution container can be fixed on the mounting part 220 of the bracket 200, the bracket 200 is accommodated in the mounting cavity 110, the bracket 200 is sleeved on the bearing piece 120 at the bottom of the inner side of the mounting cavity 110 through the sliding part 230 at the bottom of the mounting plate 210, the bracket 200 can make the sliding part 230 perform linear translation motion on the bearing piece 120 under the driving of external force, so that the cell culture solution container is driven by the bracket 200 to extend out or retract into the mounting cavity 110, and the bracket 200 has a wider space after extending out of the mounting cavity 110, thereby facilitating the fixed placement of the cell culture solution container on the bracket 200; meanwhile, the disassembly and replacement are convenient, and when the cell culture solution container is connected with the peristaltic pump through the infusion tube, the ordered operation of the infusion tube is also convenient. Meanwhile, the cell culture solution container storage chamber needs to have a large enough space so that different nutrient components or cell factors can be manually injected through a bacterial filter on the compound-hole bottle cap of the culture bottle in different culture time periods, and accurate control of cell culture conditions is realized. Compared with the prior art, the cell culture solution container storage device can be conveniently and fixedly placed in a cell culture instrument, is convenient to detach and replace, has a large operation space, improves the use convenience and the cell culture work efficiency, and can realize the accurate control of culture conditions.
The invention is also realized by the following technical scheme.
In the embodiment of the present application, a liquid level sensor 140 is further included, and the liquid level sensor 140 is disposed on the mounting plate 210 on the side of the mounting portion 230. In this embodiment, a liquid level sensor 140 is disposed beside each mounting portion 230, the liquid level sensor 140 is electrically connected to a control module in the incubator, and the control module may be a processor or a controller integrated in the incubator. Liquid level sensor 140 is arranged in obtaining the liquid level variation in the culture solution container to give control module with data signal transmission, control module carries out external data signal output through analysis processes, and these data signal can show or alarm module carries out the police dispatch newspaper through the display screen on the culture apparatus, with the change of suggestion culture solution container liquid level volume, thereby remind operating personnel to handle the culture solution in the container.
Specifically, when the splendid attire was the culture solution that cell culture used in the culture solution container, liquid level sensor 140 detected that the liquid level volume is in when the minimum critical value, promptly can not satisfy when normal cell culture used, detected signal is received and is handled the back by control module, can show through the display screen or carry out the police dispatch newspaper through alarm module, reminds the experimenter to supply the culture solution of interpolation after the culture solution consumes certain degree to avoid cultivating the interrupt. When the splendid attire was the produced cell waste liquid of cell culture in the culture solution container, liquid level sensor 140 detected the liquid level volume when being in the highest critical value, when the cell waste liquid had the excessive risk of taking place promptly, detected signal is received and is handled the back by control module, can show through the display screen or carry out the police dispatch newspaper through alarm module, and the suggestion laboratory staff carries out in time discharging the cell waste liquid in the culture solution container, avoids the cell waste liquid to be excessive from the culture solution container.
The bottom of the inner side of the mounting cavity 110 is fixedly provided with a power source 300, and the power source 300 is used for driving the sliding part 230 at the bottom of the mounting plate 210 to make linear translation motion on the bearing member 120. The power source 300 can provide a horizontal driving force for the mounting plate 210, and under the action of the horizontal driving force, the sliding part 230 at the bottom of the mounting plate 210 can make a linear translational motion along the bearing 120 under the synergistic effect of the force, so as to push the bracket 200 out of the mounting cavity 110 or pull the bracket 200 into the mounting cavity 110, thereby realizing the mounting or dismounting of the cell culture solution container through the bracket 200. By adopting the starting of the power source 300, the bracket 200 can be controlled to automatically extend out of or retract into the mounting cavity 110, so that the mounting and dismounting of the cell culture solution container are facilitated.
Specifically, referring to fig. 3, as a first embodiment of the present invention, the power source 300 is a telescopic mechanism 310, the telescopic mechanism 310 is any one of a hydraulic cylinder, an air cylinder, or an electric cylinder, a fixed end of the telescopic mechanism 310 is fixedly connected to the bottom of the inner side of the mounting cavity 110, a movable end of the telescopic mechanism 310 is connected to the mounting plate 210, and the sliding portion 230 is driven to perform a linear translational motion on the bearing member 120 by the elongation of the movable end of the telescopic mechanism 310. When a hydraulic cylinder or an air cylinder is selected as the power source 300, the hydraulic cylinder, the air cylinder or the electric cylinder can be controlled by a PLC controller, the PLC programming is adopted to control the hydraulic cylinder, the air cylinder or the electric cylinder in the prior art, the telescopic mechanism 310 is adopted as the power source 300, when the bracket 200 needs to act, the PLC controller controls the telescopic amount of the movable end of the telescopic mechanism 310 by starting a power switch, so that the driving force is provided for the mounting plate 210, the sliding part 230 is driven by the mounting plate 210 to do linear translation motion along the bearing part 120, so that the bracket 200 is pushed out of the mounting cavity 110, or the bracket 200 is retracted into the mounting cavity 110, and the storage, taking and replacement of the cell culture solution container in the mounting cavity 110 are realized. By the start of the power source 300, the bracket 200 can be controlled to automatically move in and out of the mounting cavity 110, thereby facilitating the mounting and dismounting of the cell culture solution container.
In the above embodiment, the carrier 120 is a pair of sliding rods 121 fixedly disposed at the bottom inside the mounting cavity 110, the sliding rods 121 may be cylindrical or square, and the sliding part 230 is provided with a sliding slot matching with the sliding rods 121, through which the sliding in the sliding part 230 can slide on the surface of the sliding rods 121.
Referring to fig. 4, as a second embodiment of the present invention, the carrier 120 is a pair of screws 122 disposed at the bottom inside the mounting chamber 110, the power source 300 is a servo motor 320, an output shaft of the servo motor 320 is fixedly connected to one end of the screw 122, and the sliding part 230 is screwed on the screw 122. In this embodiment, the servo motor 320 is fixedly disposed at the bottom of the inner side of the mounting cavity 110, the output shaft of the servo motor 320 is connected with one end of the screw rod 122 through the coupling, the rotation direction of the output shaft of the servo motor 320 is controlled, the rotation direction of the screw rod 122 is controlled, so that the screw rod 122 rotates to drive the sliding part 230 on the screw rod 122 to move, the output of displacement can be accurately performed by controlling the servo motor 320, the servo motor 320 is used as the power source 300, the position of the sliding part 230 moving on the bearing part 120 can be accurately controlled, the bracket 200 can conveniently extend out of the predetermined position from the mounting cavity 110, and the mounting and dismounting of the cell culture solution container are facilitated.
Referring to fig. 1, as a third embodiment of the present invention, the bracket 200 further includes a blocking plate 240 vertically connected to the mounting plate 210, the blocking plate 240 covering the opening of the mounting cavity 110. The outer side wall of the baffle 240 is provided with a handle 250, and the bracket 200 extends out of or retracts into the mounting cavity 110 under the driving of the handle 250. According to the invention, on the premise that the power source 300 is not installed, an external force can be applied to the bracket 200 in a manual mode, specifically, the handle 250 is pulled to drive the bracket 200 to linearly move in a translational manner in the installation cavity 110, so that the bracket 200 can be freely opened and closed, when the bracket 200 is completely retracted into the installation cavity 110, the opening of the installation cavity 110 is covered by the baffle 240, and meanwhile, the baffle 240 plays a limiting role, so that the installation and the disassembly of a cell culture solution container on the bracket 200 are facilitated. In the present embodiment, the structures of the sliding part 230 and the carrier 120 are the same as those of the first embodiment.
The embodiment of the invention also comprises the following technical scheme:
referring to fig. 3 and 4, a limiting portion 130 is fixedly disposed at the bottom of the inner side of the mounting cavity 110, one end of the bearing member 120 is inserted into the side wall of the opening of the mounting cavity 110, and the other end of the bearing member 120 is inserted into the limiting portion 130.
Specifically, in the first and third embodiments, since the bearing member 120 is the sliding rod 121, and the sliding portion 230 moves linearly and translationally along the sliding rod 121, the sliding rod 121 is fixed at the bottom inside the mounting cavity 110, that is, in practical implementation, one end of the sliding rod 121 is fixed on the opening side wall of the mounting cavity 110, and the other end of the sliding rod 121 is fixed on the limiting portion 130, so that the sliding rod 121 is fixed and can keep the sliding portion 230 moving in the radial direction of the sliding rod 121, and when the sliding portion 230 moves to the outside of the mounting cavity 110 along the sliding rod 121, the sliding portion 230 can be limited by the opening side wall of the mounting cavity 110 when moving to the opening close to the mounting cavity 110; when the sliding part 230 moves along the sliding rod 121 toward the inside of the mounting cavity 110, the sliding part 230 can be limited by the limiting part 130. Of course, in the first embodiment, the extension amount of the telescopic mechanism 310 may be programmed by PLC control, or may play a role of limiting.
In the second embodiment, because the bearing component 120 is the screw rod 122, the sliding part 230 performs linear translation motion on the screw rod 122 along with the rotation of the screw rod 122, in practical implementation, one end of the screw rod 122 is rotatably connected to the side wall of the opening of the mounting cavity 110, and the other end of the screw rod 122 is rotatably connected to the limiting part 130, so that the other end of the screw rod 122 passes through the limiting part 130 and is connected with the output shaft of the servo motor 320, and the control of the screw rod 122 by the servo motor 320 can be realized, so that the sliding part 230 is driven to move on the screw rod 122, and when the sliding part is moved to a position close to the opening of the mounting cavity 110, the sliding part 230 can be limited by the side wall of the opening of the; when the sliding part 230 moves along the screw 122 toward the inside of the mounting cavity 110, the sliding part 230 can be limited by the limiting part 130. Of course, in the second embodiment, the pulse signal set by the servo motor 320 can precisely control the rotation rate of the screw rod 122, thereby controlling the linear displacement of the sliding part 230 and also playing a role in limiting.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions, improvements, etc. within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A culture solution container storage device in a three-dimensional perfusion type cell culture instrument is characterized by comprising:
the incubator comprises a culture apparatus shell (100), wherein installation cavities (110) are arranged on two sides of the culture apparatus shell, and bearing parts (120) are arranged at the bottoms of the inner sides of the installation cavities (110);
the bracket (200) is provided with a horizontally arranged mounting plate (210), the upper top surface of the mounting plate (210) is provided with at least one mounting part (220) for placing a cell culture solution container, the bottom surface of the mounting plate (210) is fixedly provided with a sliding part (230), and the sliding part (230) is sleeved on the bearing piece (120);
the bracket (200) is driven by external force to do reciprocating linear translation motion on the bearing piece (120) through the sliding part (230) so as to drive the bracket (200) to extend out or retract into the mounting cavity (110).
2. The culture solution container storage device in a three-dimensional perfusion cell culture instrument according to claim 1, wherein: the liquid level sensor (140) is further included, and the liquid level sensor (140) is arranged on the mounting plate (210) on one side of the mounting portion (220).
3. The culture solution container storage device in a three-dimensional perfusion cell culture instrument according to claim 1, wherein: the bottom of the inner side of the mounting cavity (110) is fixedly provided with a power source (300), and the power source (300) is used for driving the sliding part (230) at the bottom of the mounting plate (210) to do linear translation motion on the bearing piece (120).
4. The culture solution container storage device in a three-dimensional perfusion cell culture instrument according to claim 3, wherein: the power supply (300) is telescopic machanism (310), the stiff end of telescopic machanism (310) with installation cavity (110) inboard bottom is fixed connection, the expansion end of telescopic machanism (310) with mounting panel (210) are connected, through the flexible volume of the expansion end of telescopic machanism (310) is in order to drive sliding part (230) is in do rectilinear translation motion on bearing piece (120).
5. A culture solution container storage device in a three-dimensional perfusion cell culture apparatus according to any one of claim 3, wherein: the bearing piece (120) is a pair of sliding rods (121) fixedly arranged at the bottom of the inner side of the mounting cavity (110).
6. The culture solution container storage device in a three-dimensional perfusion cell culture instrument according to claim 4, wherein: the telescopic mechanism (310) is any one of a hydraulic cylinder, an air cylinder or an electric cylinder.
7. The culture solution container storage device in a three-dimensional perfusion cell culture instrument according to claim 3, wherein: the bearing piece (120) is a pair of screw rods (122) fixedly arranged at the bottom of the inner side of the mounting cavity (110), the power source (300) is a servo motor (320), an output shaft of the servo motor (320) is fixedly connected with one end of each screw rod (122), and the sliding part (230) is in threaded connection with the screw rods (122).
8. The culture solution container storage device in a three-dimensional perfusion cell culture instrument according to claim 1, wherein: the bracket (200) further comprises a baffle (240) vertically connected with the mounting plate (210), and the baffle (240) is used for covering the opening of the mounting cavity (110).
9. The culture solution container storage device in a three-dimensional perfusion cell culture instrument according to claim 8, wherein: the outer side wall of the baffle plate (240) is provided with a handle (250), and the bracket (200) extends out of or retracts into the mounting cavity (110) under the driving of the handle (250).
10. The culture solution container storage device in a three-dimensional perfusion cell culture instrument according to claim 1, wherein: fixed spacing portion (130) that is provided with on installation cavity (110) inboard bottom, the one end that holds carrier (120) is rotated and is connected on installation cavity (110) opening lateral wall, the other end that holds carrier (120) is rotated and is connected on spacing portion (130).
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| CN201911035492.7A CN110684658A (en) | 2019-10-29 | 2019-10-29 | Culture solution container storage device in three-dimensional perfusion type cell culture instrument |
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Application publication date: 20200114 |