CN106520539A - Multi-function instrument used for microbe acid and gas producing experiment and sulfur-containing amino acid detection experiment - Google Patents
Multi-function instrument used for microbe acid and gas producing experiment and sulfur-containing amino acid detection experiment Download PDFInfo
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- CN106520539A CN106520539A CN201611102571.1A CN201611102571A CN106520539A CN 106520539 A CN106520539 A CN 106520539A CN 201611102571 A CN201611102571 A CN 201611102571A CN 106520539 A CN106520539 A CN 106520539A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/04—Bioreactors or fermenters specially adapted for specific uses for producing gas, e.g. biogas
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/36—Means for collection or storage of gas; Gas holders
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
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Abstract
The invention relates to the field of microbe experimental facilities, and in particular discloses a multi-function instrument used for the microbe acid and gas producing experiment and the sulfur-containing amino acid detection experiment. The multi-function instrument comprises a constant temperature incubator, a fermentation test tube, an acid and gas producing experiment indicating device and a sulfur-containing amino acid detection device, wherein the acid and gas producing experiment indicating device comprises a liquid storage chamber and a seal end cover, the middle part of the seal end cover sinks, the sinking part is in sealed connection with a gas collecting tube, a water injecting hole and an air hole are formed in the seal end cover, and a puncture device and a seal rubber sleeve are in sealed connection to the bottom of the liquid storage chamber; the sulfur-containing amino acid detection device comprises a detection chamber, a rubber plug is plugged to the lower opening of the detection chamber, a puncture device is inserted into the rubber plug, and a rubber seal sleeve is arranged at the lower end of the detection chamber; a filter paper strip coiled to form the barrel shape is plugged in the detection chamber. The multi-function instrument has the advantages that the size is small, the taking for use is convenient, the operation is simple, the efficiency is high, the space is saved, the experiment is simple and rapid, the result is reliable, and multiple purposes are realized.
Description
（One）Technical field
The present invention relates to Experiment on Microbiology apparatus field, sour aerogenesis experiment is produced in more particularly to a kind of microorganism and sulfur-containing amino acid is examined
Survey the multiplex instrument of experiment.
（Two）Background technology
At present, when the sour aerogenesis of product for carrying out the fermentables such as glucose and Lactose is tested because the generation feelings of gas are observed
Condition, often will be inverted a minimum fermentation tubule of bore in the Boiling tube for fill fluid medium, and operation is very loaded down with trivial details,
Fluid medium is filled with into the tubule that ferments with pipet, is then reversed fast and is had been loaded with the Boiling tube of fluid medium,
Even if meticulous, careless manipulation can also produce bubble, at this moment just must take out fermentation tubule and reappose, waste time and energy,
Effect is bad；Simultaneously because containing different saccharides in fermentation broth（Such as glucose or Lactose）, once test and terminate
Afterwards, as the tubule bore that ferments is less, it is difficult to be rinsed with test tube brush, such as thoroughly rinsed and wasted very much with washing liquid etc. every time
Time and efforts, such as can not be rinsed well, once experiment will be had a strong impact on containing a small amount of glucose in lactose fermentation tubule
As a result, make originally should not the experiment of aerogenesis there are the false positive results of aerogenesis.
During sulfur-containing amino acid test experience, typically a small amount of FeSO is added when solid or semisolid culturemedium is prepared4Or vinegar
Lead plumbate is (because H2S and FeSO4Or lead acetate effect can generate the FeS or vulcanized lead precipitation of black), by percutaneous puncture-inoculation, see through
Black generation is whether there is around thorn line, if any just illustrating that the aminoacid is sulfur-containing amino acid；Such as puncture no black around line to produce,
Then illustrate that the aminoacid is free from sulfur aminoacid；But due to by FeSO4Or after lead acetate adds culture medium, even if the H for producing2S is measured
Much, it is also difficult to see that black occurs, a small amount of black also more difficult observation occur so that false negative easily occur, often contain to detection
Sulfur aminoacid brings difficulty.
（Three）The content of the invention
The present invention is in order to make up the defect of prior art, there is provided sour aerogenesis is produced in a kind of microorganism of simple to operate, effect is significant
Experiment and the multiplex instrument of sulfur-containing amino acid test experience.
The present invention is achieved through the following technical solutions：
Sour aerogenesis experiment and the multiplex instrument of sulfur-containing amino acid test experience, including constant incubator and fermentation examination are produced in a kind of microorganism
Pipe, is characterized in that, also including the sour aerogenesis experiment instruction device of product and sulfur-containing amino acid detection means；Wherein,
The acid aerogenesis experiment instruction device of producing includes columnar liquid storage room, and the liquid storage room is provided with sealed end
Lid, the end cover mid portion sink, and countersink is tightly connected downward opening discharge, sets on the end cover
There are water injection hole and passage, the water injection hole and passage are designed with closure；Liquid storage room sealed bottom is connected with
Sting device, the sting device include duroplastss breather and piercing needle, and the duroplastss breather stretches into liquid storage
Chamber interior, is cased with sealing rubber cover in liquid storage room lower end, and the sealing rubber cover can be by liquid storage room and fermentation tube
It is tightly connected, after being tightly connected, duroplastss breather top is located at discharge top；
The sulfur-containing amino acid monitoring device includes downward opening sensing chamber, and the sensing chamber Xia Kou is plugged with rubber stopper, rubber
Perforator is inserted with beyond the Great Wall, and the perforator includes hard plastic tube and puncture needle, and the upper end insertion detection of the hard plastic tube is indoor,
The sensing chamber lower end is provided with rubber sealing boot, and sensing chamber and fermentation tube can be tightly connected by the rubber sealing boot；It is described
Detection interior is plugged with the filter paper bar for being rolled into tubular, the filter paper bar through mass concentration for 1-2% ferrous sulfate or lead acetate water
After solution soaking, drying, tubular is rolled into, then fills in sensing chamber；
Also include fermentation tube screen work, the fermentation tube screen work includes base, screen work and screen work lid, if being provided with the base
The dry screen work groove that can accommodate screen work, the screen work are divided into some test tube lattice that can accommodate fermentation tube, and each screen work is supporting
One screen work lid.
The top of the discharge is not higher than the top of end cover.
The base two ends are provided with the cross-arm that section is polygon or ellipse, on the wall of in the constant incubator a side
Driving shaft is provided with, the side wall is hollow structure, and the driving shaft connects one by lever after stretching in hollow structure, described by lever
It is vertical with driving shaft, be provided with motor in hollow structure, the outfan connecting eccentric wheel of the motor, the eccentric with it is passive
Bar is contacted, and described to be connected back to a spring by lever, the other end of the return spring is fixed on the wall of hollow structure, the perseverance
Another side wall in warm incubator is provided with driven shaft, be respectively provided with the driving shaft and driven shaft one it is supporting with cross-arm
Groove.
The end cover is provided with observation port.
The invention has the beneficial effects as follows：
1st, when carrying out producing sour aerogenesis test, do not contact with culture medium due to producing sour aerogenesis experiment instruction device, and in liquid
Substantial amounts of strain is accessed, so the system is without sterilizing, miscellaneous bacteria infection is not resulted in yet, it is more convenient to use, wait to be equipped with
After the Boiling tube of culture medium sterilizes and is inoculated with, install instruction system, you can heat insulating culture, and the water in instruction system is without more
Change, the system can be repeatedly used, the detection for producing sour experimental result is also easy to by fermentation tube culture medium color change
Observation.
2. as discharge is not contacted with the culture medium in fermentation tube, so discharge does not reach Lactose or Fructus Vitis viniferae
Sugar, would not occur occurring producing sour aerogenesis and test false-positive knot due to being mixed into glucose in lactinated fermentation tubule certainly
Really.Particularly various substrate saccharides carry out fermentation and differentiate more show its advantage during strain.
3., when the detection of sulfur-containing amino acid is carried out, fermentation tube adds fluid medium and aminoacid to be detected, plus
Sterilize after lid standby（It is identical with conventional method, due to being fluid medium, so having saved agar, cost is reduced, shortened
Incubation time）, in the process sensing chamber be not required to contact culture medium with the puncture needle in which, filter paper, so need not all go out
Bacterium, makes detection simpler.
4. culture medium prepares simpler, has saved agar；Experimental result is more easily determined, is not in false negative result；
The strain being linked in culture medium will not also be subject to FeSO4Or the adverse effect of lead acetate, realize detectable and send out with culture
Ferment system is effectively isolated, it is easier to desired result occur；Sensing chamber etc. without the need for sterilizing, has saved the time, and the device has
Small volume, takes conveniently, and simple to operate, efficiency is higher, saves space, makes experiment more simple and fast, as a result more reliable.
5. the system can very easily connect gas analyses detecting instrument due to the pipeline collection for carrying out gas, can
More intuitively, faster to carry out Real_time quantitative detection and analysis.
6. the multiplex instrument, can also enter to act charitably so decapacitation is carried out outside the fermenting experiment of anaerobe due to having added oscillation device
The culture and analysis of oxygen bacterium, realizes multi-purpose function.
（Four）Description of the drawings
The present invention is further illustrated below in conjunction with the accompanying drawings：
Cross section structure diagrams of the Fig. 1 for constant incubator；
Fig. 2 is the enlarged drawing at A in Fig. 1；
Fig. 3 is fermentation tube and produces the cross section structure diagram after sour aerogenesis experiment instruction device is tightly connected；
Fig. 4 is the cross section structure diagram after fermentation tube and sulfur-containing amino acid detection means are tightly connected；
Main structure diagrams of the Fig. 5 for base；
Overlooking the structure diagrams of the Fig. 6 for base；
Overlooking the structure diagrams of the Fig. 7 for screen work；
Main structure diagrams of the Fig. 8 for screen work lid.
In figure, 1 constant incubator, 2 fermentation tubes, 3 driving shafts, 4 hollow structures, 5 by lever, 6 return springs, and 7 is driven
Axle, 8 grooves, 9 motors, 10 eccentrics, 11 liquid storage rooms, 12 end covers, 13 countersinks, 14 water injection holes, 15 passages,
16 discharges, 17 observation ports, 18 duroplastss breathers, 19 piercing needles, 20 sealing rubber covers, 21 sensing chamber, 22 rubber stoppers, 23
Hard plastic tube, 24 puncture needles, 25 filter paper bars, 26 bases, 27 screen work grooves, 28 screen works, 29 test tube lattice, 30 screen work lids, 31 cross-arms, 32
Rubber sealing boot, 33 closures.
（Five）Specific embodiment
Accompanying drawing is the specific embodiment of the present invention.As shown in Figures 1 to 8, sour aerogenesis experiment and sulfur-bearing amino are produced in this kind of microorganism
The multiplex instrument of sour test experience, including constant incubator 1, fermentation tube 2, produce sour aerogenesis experiment instruction device and sulfur-containing amino acid inspection
Survey device；Wherein,
One of side wall of constant incubator 1 is hollow structure 4, and hollow structure 4 is towards on the wall of the cavity of constant incubator 1
One driving shaft 3 is installed, driving shaft 3 is located at the part in hollow structure 4 and connects one by lever 5, by lever 5 and driving shaft 3
Vertically, being connected a return spring 6 in lever 5, the fixing end of return spring 6 be fixed on on 3 same wall of driving shaft,
One motor 9 is also installed in hollow structure 4, the outfan of motor 9 connects an eccentric 10, eccentric 10 all the time with passively
Bar 5 is contacted, and return spring 6 is all located at by the lower section of lever 5 with motor 9；One is installed on another side wall of constant incubator 1
Groove 8 is provided with individual driven shaft 7, driving shaft 3 and driven shaft 7 all, the cross section of groove 8 is polygon or ellipse, when groove 8
It is opening up when, now by lever 5 be in horizontality, return spring 6 be in semi-drawing state, when the nearly circle of eccentric 10
When heart point is with being contacted by lever 5, return spring 6 is in relaxed state, and now the opening of groove 8 is oblique upward.
Producing sour aerogenesis experiment instruction device includes columnar liquid storage room 11, and 11 upper end of liquid storage room is provided with sealing
End cap 12,12 mid portion of end cover sink, and countersink 13 is tightly connected downward opening discharge 16, that is, is commonly called as
Fermentation tubule, the top of end cover 12 is higher than the top of discharge 16, concordant with the top of discharge 16 at least,
So that 16 interior energy of discharge is full of water, end cover 12 is provided with observation port 17, water injection hole 14 and passage 15,14 He of water injection hole
Passage 15 is all configured with closure 33, and observation port 17 will can guarantee that observes gas production in discharge 16；Liquid is stored up
11 sealed bottom of standby room is connected with sting device, and sting device includes the duroplastss breather 18 being made into integration and piercing needle
19, duroplastss breather 18 is stretched into inside liquid storage room 11, is cased with sealing rubber cover 20 in 11 lower end of liquid storage room, sealing
Tubular of the rubber sleeve 20 for upper and lower ends opening, its top half part fixed cover is in 11 lower end of liquid storage room, the latter half
Fermentation tube 2 can be inserted in be tightly connected liquid storage room 11 and fermentation tube 2, after being tightly connected, full of distillation in discharge
Water, 18 top of duroplastss breather are located at 16 top of discharge, it is to avoid distilled water is flowed down from discharge, but is easy to gas from collection
Trachea flows out；
Sulfur-containing amino acid monitoring device includes downward opening sensing chamber 21, and sensing chamber 21 can be replaced with short test tube, sensing chamber 21
Lower mouth is plugged with rubber stopper 22, is inserted with perforator on rubber stopper 22, and as perforator with sting device is, perforator includes one
Made by hard plastic tube 23 and puncture needle 24, hard plastic tube 23 upper end insertion sensing chamber 21 in, 21 lower end of sensing chamber is provided with rubber
Glue sealing shroud 32, rubber sealing boot 32 and the structure of sealing rubber cover 20, shape are identical with effect, repeat no more；In sensing chamber
The filter paper bar 25 for being rolled into tubular is plugged with 21, filter paper bar 25 is water-soluble for the ferrous sulfate or lead acetate of 1-2% through mass concentration
After immersion bubble, drying, tubular is rolled into, then fills in sensing chamber 21；
Also include fermentation tube screen work, fermentation tube frame is used to place and sending out that liquid storage room 11 or sensing chamber 21 assemble
Ferment test tube 2, it includes base 26, screen work 28 and screen work lid 30, and some screen work grooves 27 that can accommodate screen work 28 are provided with base 26,
Each screen work groove 27 places a screen work 28, and screen work 28 is divided into some test tube lattice 29 that can accommodate fermentation tube 2, each lattice
28 supporting screen work lids 30 of frame, the fermentation tube 2 containing culture medium is put on screen work 28 can effectively be fastened with screen work lid 30, side
Just carry out high pressure steam sterilization and move into superclean bench carrying out the sterile working such as being inoculated with；26 two ends of base are integrally formed with section and are
The cross-arm 31 of polygon or ellipse, cross-arm 31 are snapped in the groove 8 of constant incubator 1 and make fermentation tube screen work in the band of motor 9
Move back and forth under dynamic.
It is as follows using process：
Produce sour aerogenesis experiment：First distilled water is injected into liquid storage room 11 and discharge 16, then will be end cover 12 quick
Good seal on liquid storage room 11 is covered, distilled water is now full of in discharge 16 and in handstand state, it is also possible to first will sealing
End cap 12 is covered on liquid storage room 11, then from water injection hole 14 to water filling in liquid storage room 11, until filling in discharge 16
Full distilled water does not have bubble and exists, and then seals water injection hole 14 and passage 15 respectively with closure 33, by piercing needle
On rubber stopper on 19 insertion fermentation tubes 2, and the latter half of sealing rubber cover 20 is made to be set on fermentation tube 2, Ran Houfang
To in test tube lattice 29, after test tube lattice 29 are all piled, screen work lid 30 is covered, be put in screen work groove 27, each screen work groove 27 is piled
Afterwards, the cross-arm 31 at 26 two ends of base is put in the groove 8 of constant incubator 1, starts motor 9 and constant incubator 1, started real
Test work.Can be by checking fermentation tube 2 after culture 24-48 hours in constant incubator 1（Containing bromocresol purple）Color,
Can just judge to produce sour situation；Become yellow explanation fermentation system and produce acid, still acid is not produced for purple explanation.Checked by observation port 17 and collected
Bubble-free is arranged at 16 top of trachea（If any bubble, then fermentation system aerogenesis, otherwise not aerogenesis）, due to without by fermentation tube 2
In fluid medium observation, as a result become readily apparent from.Do not contact with culture medium due to producing sour aerogenesis experiment instruction device, and liquid
Substantial amounts of strain has been accessed in body, so sour aerogenesis experiment instruction device is produced without sterilizing, is not also resulted in miscellaneous bacteria infection, is used
Get up convenient, after the fermentation tube equipped with culture medium sterilizes and is inoculated with, install the sour aerogenesis experiment instruction device of product, i.e.,
Can heat insulating culture, and produce the water in sour aerogenesis experiment instruction device without changing so that the device can be repeatedly used.Also may be used
By connecting flexible pipe in piercing needle opening, discharge is filled into water first with wash bottle, then is added water by water injection hole.
Sulfur-containing amino acid test experience：After filter paper bar 25 Jing after processing fills in sensing chamber 21, puncture needle 24 is inserted and is sent out
On the rubber stopper of ferment test tube 2, sensing chamber 21 and fermentation tube 1 are tightly connected with rubber sealing boot 33, are then placed in base
On 26, place into and can just test in constant incubator 1.As long as there is the point of black during observation on filter paper bar, then sulfur is illustrated
Change hydrogen and produce the test positive, otherwise hydrogen sulfide produces negative.Due to sensing chamber with its in puncture needle, filter paper bar is not required to
Contact culture medium, so need not all sterilize, makes detection simpler.
The present invention is collected due to the pipeline for carrying out gas, can very easily connect gas analyses detecting instrument, can be with
More intuitively, Real_time quantitative detection and analysis are faster carried out.
The present invention can also carry out aerobic bacteria due to having added oscillation device so decapacitation is carried out outside the fermenting experiment of anaerobe
Culture and analysis, realize multi-purpose function.
In addition to technical characteristic described in description, remaining technical characteristic is technology known to those skilled in the art.
Claims (4)
1. sour aerogenesis experiment and the multiplex instrument of sulfur-containing amino acid test experience, including constant incubator are produced in a kind of microorganism（1）With send out
Ferment test tube（2）, it is characterized in that, also including the sour aerogenesis experiment instruction device of product and sulfur-containing amino acid detection means；Wherein,
The acid aerogenesis experiment instruction device of producing includes columnar liquid storage room（11）, the liquid storage room（11）It is provided with
End cover（12）, the end cover（12）Mid portion sinks, countersink（13）It is tightly connected downward opening gas collection
Pipe（16）, the end cover（12）It is provided with water injection hole（14）And passage（15）, the water injection hole（14）And passage
（15）It is designed with closure（33）；The liquid storage room（11）Sealed bottom is connected with sting device, the sting device bag
Include duroplastss breather（18）And piercing needle（19）, the duroplastss breather（18）Stretch into liquid storage room（11）Inside,
In liquid storage room（11）Lower end is cased with sealing rubber cover（20）, the sealing rubber cover（20）Can be by liquid storage room（11）With
Fermentation tube（2）It is tightly connected, after being tightly connected, duroplastss breather（18）Top is located at discharge（16）Top；
The sulfur-containing amino acid monitoring device includes downward opening sensing chamber（21）, the sensing chamber（21）Lower mouth is plugged with rubber
Plug（22）, rubber stopper（22）On be inserted with perforator, the perforator includes hard plastic tube（23）And puncture needle（24）, the hard plastic
Expects pipe（23）Upper end insertion sensing chamber（21）It is interior, the sensing chamber（21）Lower end is provided with rubber sealing boot（32）, the rubber
Sealing shroud（32）Can be by sensing chamber（21）And fermentation tube（2）It is tightly connected；The sensing chamber（21）Inside it is plugged with and is rolled into tubular
Filter paper bar（25）, the filter paper bar（25）Through mass concentration for 1-2% ferrous sulfate or lead acetate water solution soaking, drying
Afterwards, tubular is rolled into, then fills in sensing chamber（21）；
Also include fermentation tube screen work, the fermentation tube screen work includes base（26）, screen work（28）With screen work lid（30）, it is described
Base（26）On be provided with and some can accommodate screen work（28）Screen work groove（27）, the screen work（28）It is divided into some can receiving to send out
Ferment test tube（2）Test tube lattice（29）, each screen work（28）A supporting screen work lid（31）.
2. sour aerogenesis experiment and the multiplex instrument of sulfur-containing amino acid test experience, its feature are produced in microorganism according to claim 1
It is, the discharge（16）Top be not higher than end cover（12）Top.
3. sour aerogenesis experiment and the multiplex instrument of sulfur-containing amino acid test experience, its feature are produced in microorganism according to claim 1
It is, the base（26）Two ends are provided with section for polygon or the cross-arm of ellipse（31）, the constant incubator（1）Interior one
Side wall is provided with driving shaft（3）, the side wall is hollow structure（4）, the driving shaft（3）Stretch into hollow structure（4）Connect after interior
One by lever（5）, it is described by lever（5）With driving shaft（3）Vertically, in hollow structure（4）Motor is provided with inside（9）, the motor
（9）Outfan connecting eccentric wheel（10）, the eccentric（10）With by lever（5）Contact, it is described by lever（5）Connection return
Spring（6）, the return spring（6）The other end be fixed on hollow structure（4）Wall on, the constant incubator（1）Interior
Another side wall is provided with driven shaft（7）, the driving shaft（3）And driven shaft（7）On be respectively provided with one and cross-arm（31）It is supporting
Groove（8）.
4. sour aerogenesis experiment and the multiplex instrument of sulfur-containing amino acid test experience, its feature are produced in microorganism according to claim 1
It is, the end cover（12）It is provided with observation port（17）.
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| CN109507178A (en) * | 2018-12-25 | 2019-03-22 | 菏泽学院 | A kind of environment-friendly microbial indole test instrument |
| CN110057969A (en) * | 2019-05-28 | 2019-07-26 | 菏泽学院 | A kind of BOD5Measure automatic medicine adding apparatus |
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|---|---|---|---|---|
| CN106916738A (en) * | 2017-04-10 | 2017-07-04 | 林春芳 | A kind of superclean bench auxiliary experiment device |
| CN109507178A (en) * | 2018-12-25 | 2019-03-22 | 菏泽学院 | A kind of environment-friendly microbial indole test instrument |
| CN109507178B (en) * | 2018-12-25 | 2021-03-19 | 菏泽学院 | Environment-friendly microbial indole tester |
| CN110057969A (en) * | 2019-05-28 | 2019-07-26 | 菏泽学院 | A kind of BOD5Measure automatic medicine adding apparatus |
| CN110057969B (en) * | 2019-05-28 | 2023-12-22 | 菏泽学院 | BOD (BOD) 5 Automatic dosing device for measurement |
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|---|---|
| CN106520539B (en) | 2017-11-28 |
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